scholarly journals Anti-Gastric Ulcer Activity of the Water Extract from Payawanorn (Pseuderanthemum Palatiferum)

2018 ◽  
Vol 36 (2) ◽  
pp. 89
Author(s):  
Kannika Inchab ◽  
Parirat Khonsung ◽  
Natthakarn Chiranthanut ◽  
Puongtip Kunanusorn ◽  
Saranyapin Potikanond ◽  
...  
Keyword(s):  
Gastric Ulcer ◽  
Alcian Blue ◽  
Water Immersion ◽  
Gastric Wall ◽  
Water Extract ◽  
Gastric Ulcers ◽  
Control Group ◽  
P Value ◽  
Ulcer Formation ◽  
Traditional Use

Objective: To examine the anti-gastric ulcer activity of Payawanorn (Psuderanthemum palatiferum)  water extract (PPE) in rats.Material and Methods: Evaluation of anti-gastric ulcer activity of PPE was performed using three induced gastric ulcers models: (1) ethanol/hydrochloric acid (EtOH/HCl), (2) restraint water immersion stress and (3) indomethacin. Ulcer indices were determined by microscopic examination (10X). Pylorus ligation and gastric-wall mucus determination were used to investigate the mechanism of anti-gastric ulcer activity of PPE.Results: Oral administration of PPE (150, 300 and 600 mg/kg) significantly inhibited gastric ulcer formation induced by EtOH/HCl, by water immersion restraint stress, and by indomethacin in rats (p-value<0.05). PPE at 600 mg/kg showed a high percent inhibition of gastric ulcer formation in all models. In the pylorus-ligated model, pretreatment with PPE had no effect on pH, acidity output or the gastric acid secretion rate, demonstrating that PPE lacks an anti-secretory effect. Gastric wall mucus was markedly preserved by pretreatment PPE at 600 mg/kg (18.91±2.57 μg alcian blue/g wet stomach) after ethanol-induced ulcer when compared to that the control group (12.38±1.02 μg alcian blue/ g wet stomach).Conclusion: PPE possesses an anti-gastric ulcer effect related to the preservation of gastric mucus, supporting the traditional use of P. palatiferum to treat gastric ulcers.

scholarly journals Anti-Gastric Ulcer Activity of the Water Extract from Payawanorn (Pseuderanthemum Palatiferum)

2018 ◽  
Vol 36 (2) ◽  
pp. 89
Author(s):  
Kannika Inchab ◽  
Parirat Khonsung ◽  
Natthakarn Chiranthanut ◽  
Puongtip Kunanusorn ◽  
Saranyapin Potikanond ◽  
...  
Keyword(s):  
Gastric Ulcer ◽  
Alcian Blue ◽  
Water Immersion ◽  
Gastric Wall ◽  
Water Extract ◽  
Gastric Ulcers ◽  
Control Group ◽  
P Value ◽  
Ulcer Formation ◽  
Traditional Use

Objective: To examine the anti-gastric ulcer activity of Payawanorn (Psuderanthemum palatiferum)  water extract (PPE) in rats.Material and Methods: Evaluation of anti-gastric ulcer activity of PPE was performed using three induced gastric ulcers models: (1) ethanol/hydrochloric acid (EtOH/HCl), (2) restraint water immersion stress and (3) indomethacin. Ulcer indices were determined by microscopic examination (10X). Pylorus ligation and gastric-wall mucus determination were used to investigate the mechanism of anti-gastric ulcer activity of PPE.Results: Oral administration of PPE (150, 300 and 600 mg/kg) significantly inhibited gastric ulcer formation induced by EtOH/HCl, by water immersion restraint stress, and by indomethacin in rats (p-value<0.05). PPE at 600 mg/kg showed a high percent inhibition of gastric ulcer formation in all models. In the pylorus-ligated model, pretreatment with PPE had no effect on pH, acidity output or the gastric acid secretion rate, demonstrating that PPE lacks an anti-secretory effect. Gastric wall mucus was markedly preserved by pretreatment PPE at 600 mg/kg (18.91±2.57 μg alcian blue/g wet stomach) after ethanol-induced ulcer when compared to that the control group (12.38±1.02 μg alcian blue/ g wet stomach).Conclusion: PPE possesses an anti-gastric ulcer effect related to the preservation of gastric mucus, supporting the traditional use of P. palatiferum to treat gastric ulcers.

scholarly journals Gastroprotective Effects of Paeonia Extract Mixture HT074 against Experimental Gastric Ulcers in Rats

2019 ◽  
Vol 2019 ◽  
pp. 1-13 ◽  
Author(s):  
Young-Sik Kim ◽  
Hyo Jin Park ◽  
Hocheol Kim ◽  
Jungbin Song ◽  
Donghun Lee
Keyword(s):  
Cell Death ◽  
Gastric Ulcer ◽  
Alcian Blue ◽  
Water Immersion ◽  
Gastric Ulcers ◽  
Prostaglandin E ◽  
Gastric Lesions ◽  
Ags Cells ◽  
Gastric Secretions ◽  
Sulfhydryl Compounds

Background. Paeonia extract mixture HT074 is a standardized multiherbal mixture comprising extracts from Inula britannica flowers and Paeonia lactiflora roots, which are used to treat digestive disorders in traditional Korean medicine. This study was focused on elucidating the underlying mechanisms of the gastroprotective effects of HT074 in different gastric ulcer models. Methods. Gastric lesions were induced in rats by an HCl/EtOH solution, water immersion-restraint stress (WIRS), and indomethacin. Gastric secretions were studied in pylorus-ligated rats, while mucus secretions were assessed by measuring alcian blue-binding capacity of mucus in the rat model of HCl/EtOH-induced gastric ulcer. Additionally, the involvement of nitric oxide (NO) and sulfhydryl compounds in HT074-mediated mucosal protection was elucidated using their inhibitors, i.e., NG-nitro- L-arginine methyl ester hydrochloride (L-NAME) and N-ethylmaleimide (NEM), respectively. Furthermore, the effects on indomethacin-induced cell death and prostaglandin E2 (PGE2) levels were assessed in AGS cells. Results. Oral administration of HT074 significantly decreased gastric lesions induced by HCl/EtOH, WIRS, and indomethacin. Furthermore, it significantly decreased the volume, acidity, and total acidity of gastric juice in pylorus-ligated rats and increased the alcian blue-stained gastric mucus in HCl/EtOH-induced gastric ulcer in rats. Pretreatment with NEM abolished the gastroprotective effects of HT074, while L-NAME did not. In AGS cells, HT074 significantly reduced indomethacin-induced cell death and increased the PGE2 levels. Conclusions. These findings suggest that HT074 has gastroprotective effects against various ulcerogens, including HCl/EtOH, immersion stress, and NSAIDs. These effects are attributed to the inhibition of gastric secretions and preservation of the gastric mucosal barrier by increased mucus production, which is partially mediated through endogenous sulfhydryl compounds and PGE2. Based on these findings, we propose that HT074 may be a promising therapeutic agent for gastritis and gastric ulcer.

scholarly journals EFFECTS OF LOW DOSE EXPOSURE OF BORAX FOR 8 WEEKS ON GASTRIC ULCER FORMATION

2020 ◽  
Vol 30 (1) ◽  
pp. 8
Author(s):  
Audi Salman Faza ◽  
Linda Dewanti ◽  
Ema Qurnianingsih
Keyword(s):  
Body Weight ◽  
Gastric Ulcer ◽  
Low Dose ◽  
Food Additives ◽  
Gastric Ulcers ◽  
Control Group ◽  
High Dose ◽  
Ulcer Formation ◽  
Group 3 ◽  
Group 1

Background: Borax, a carcinogenic toxicant, is illegally used as food additives frequently. Previous researches shown that high dose of borax exposure (300 mg/kg of BW) caused gastric ulcer, but low dose of borax, as found as usual dose in food, has not been frequently researched. Objective: The aim of this study was to analyze the effects of low dose of borax for 8 weeks on the formation of gastric ulcer. Material and Method: Twenty four young adult rats (Rattus norvegicus) were divided into 4 groups (Control, Group 1, 2, and 3). Borax doses of 50 mg/kg, 150 mg/kg, and 300 mg/kg were administered on groups 1, 2, and 3 respectively through sonde for 8 weeks; Control group was not exposed with borax. Body weight was monitored every 2 weeks. On the last day of the experiment, rats were euthanized and gastric ulcers were histopathologically analyzed using a microscope. The data were analyzed by the comparative test of Mann-Whitney and the significancy was set on p <0.050. Result: The results showed that body weight was significantly different on the 6 th week between Control vs Group 1 (p= 0.010) and Control vs Group 3 (p= 0.043). The grade of gastric ulcer was found higher in Group 2 and Group 3 compared to Control (p= 0.019 and 0.011 respectively). Conclusion:  Both of low dose (150mg / kg BW) and high dose (300mg / kg BW) of borax exposure for 8 weeks cause microscopic gastric ulcer on rats.  

scholarly journals EFFECTS OF LOW DOSE EXPOSURE OF BORAX FOR 8 WEEKS ON GASTRIC ULCER FORMATION

2020 ◽  
Vol 30 (1) ◽  
pp. 7
Author(s):  
Audi Salman Faza ◽  
Linda Dewanti ◽  
Ema Qurnianingsih
Keyword(s):  
Body Weight ◽  
Gastric Ulcer ◽  
Low Dose ◽  
Food Additives ◽  
Gastric Ulcers ◽  
Control Group ◽  
High Dose ◽  
Ulcer Formation ◽  
Group 3 ◽  
Group 1

Background: Borax, a carcinogenic toxicant, is illegally used as food additives frequently. Previous researches shown that high dose of borax exposure (300 mg/kg of BW) caused gastric ulcer, but low dose of borax, as found as usual dose in food, has not been frequently researched. Objective: The aim of this study was to analyze the effects of low dose of borax for 8 weeks on the formation of gastric ulcer. Materials and Methods: Twenty four young adult rats (Rattus norvegicus) were divided into 4 groups (Control, Group 1, 2, and 3). Borax doses of 50 mg/kg, 150 mg/kg, and 300 mg/kg were administered on groups 1, 2, and 3 respectively through sonde for 8 weeks; Control group was not exposed with borax. Body weight was monitored every 2 weeks. On the last day of the experiment, rats were euthanized and gastric ulcers were histopathologically analyzed using a microscope. The data were analyzed by the comparative test of Mann-Whitney and the significancy was set on p<0.050. Results: The results showed that body weight was significantly different on the 6th week between Control vs Group 1 (p=0.010) and Control vs Group 3 (p=0.043). The grade of gastric ulcer was found higher in Group 2 and Group 3 compared to Control (p=0.019 and 0.011 respectively). Conclusion: Both of low dose (150 mg/kg BW) and high dose (300mg/kg BW) of borax exposure for 8 weeks cause microscopic gastric ulcer on rats.

Endothelin-1, an ulcer inducer, promotes gastric ulcer healing via mobilizing gastric myofibroblasts and stimulates production of stroma-derived factors

2006 ◽  
Vol 290 (5) ◽  
pp. G1041-G1050 ◽  
Author(s):  
Tsutomu Nishida ◽  
Shingo Tsuji ◽  
Arata Kimura ◽  
Masahiko Tsujii ◽  
Syuji Ishii ◽  
...  
Keyword(s):  
Gastric Ulcer ◽  
Ulcer Healing ◽  
Angiogenic Factors ◽  
Gastric Epithelium ◽  
Gastric Ulcers ◽  
Ulcer Formation ◽  
Peptic Ulcers ◽  
Converting Enzyme ◽  
Potent Inducer ◽  
Gastric Ulcer Healing

Endothelin (ET)-1 is a potent inducer of peptic ulcers. The roles of ET-1 in ulcer healing, however, have remained unclear, and these were investigated in mice. Gastric ulcers were induced in mice by serosal application of acetic acid. Three days later, mice were given a neutralizing ET-1 antibody or nonimmunized serum. The ulcer size, amount of fibrosis and myofibroblasts, and localization of ET-1 and ETA/B receptors were analyzed. To elucidate the mechanisms underlying the effects of ET-1, we examined the proliferation, migration, and release of growth and angiogenic factors in gastric myofibroblasts with or without ET-1. The expression of prepro-ET-1 (an ET-1 precursor) and ET-converting enzyme-1 was examined in gastric myofibroblasts using RT-PCR. Immunoneutralization of ET-1 delayed gastric ulcer healing. The areas of fibrosis and myofibroblasts were smaller in the anti-ET-1 antibody group than in the control. ET-1 was expressed in the gastric epithelium, myofibroblasts, and other cell types. ETA receptors, but not ETB receptors, were present in myofibroblasts. ET-1 increased proliferation and migration of gastric myofibroblasts. ET-1 stimulated the release of hepatocyte growth factor, VEGF, PGE2, and IL-6 from gastric myofibroblasts. mRNA for prepro-ET-1 and ET-converting enzyme-1 was also expressed. ET-1 promotes the accumulation of gastric myofibroblasts and collagen fibrils at gastric ulcers. ET-1 also stimulates migration and proliferation of gastric myofibroblasts and enhances the release of growth factors, angiogenic factors, and PGE2. Thus ET-1 has important roles not only in ulcer formation but also in ulcer healing via mobilizing myofibroblasts and inducing production of stroma-derived factors.

A STUDY ON THE RELATIONSHIP BETWEEN HELICOBACTER PYLORI AND TRACE ELEMENTS IN GASTRIC MUCOSA

2006 ◽  
Vol 16 (01n02) ◽  
pp. 55-68
Author(s):  
T. FUKUDA ◽  
S. SAKURAI ◽  
M. KUDO
Keyword(s):  
Helicobacter Pylori ◽  
Trace Elements ◽  
Gastric Ulcer ◽  
Gastric Mucosa ◽  
Gastric Ulcers ◽  
Control Group ◽  
Human Gastric Mucosa ◽  
H2 Receptor Antagonist ◽  
Zinc Levels ◽  
The Relationship

H2 receptor antagonist (H2RA) and proton pump inhibitor(PPI) are often used to treat gastric ulcers, in addition to the zinc-L-carcino-complex which is commonly adopted for such therapies. However, there have been no previous reports detailing the levels and distribution of zinc in human gastric mucosa. The aim of our current study is to clarify the relationship between Helicobacter pylori infection and trace elements such as zinc in gastric mucosa using particle induced X-ray emission (PIXE). Forty gastric ulcer patients were chosen as subjects of this study and divided into two groups. One is a new ulcer patients group(twenty nine cases). And the other, eleven of these patients had been prescribed zinc-L-carcino-complex (polapreZinc®), at a dose of 150mg/day for periods ranging from 16 to 20 weeks. The zinc levels in the gastric ulcer group were found to be significantly less than those of the control group and the concentrations of zinc in gastric mucosa were observed to increase following the administration of treatments containing this element.

scholarly journals Effects of Grape Seed Extract, Vitamin C, and Vitamin E on Ethanol- and Aspirin-Induced Ulcers

2011 ◽  
Vol 2011 ◽  
pp. 1-6 ◽  
Author(s):  
Vivian Molina Cuevas ◽  
Yazmín Ravelo Calzado ◽  
Yohani Pérez Guerra ◽  
Ambar Oyarzábal Yera ◽  
Sonia Jiménez Despaigne ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Gastric Ulcer ◽  
Vitamin E ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Positive Control ◽  
Negative Control ◽  
Seed Extract ◽  
Gastric Ulcers ◽  
Control Group

Effects of GSE and vitamins C and E on aspirin- and ethanol-induced gastric ulcer and associated increases of lipid peroxidation in rats were compared. Two experiments were conducted. Rats were randomized into eight groups: a negative control and seven groups that received aspirin or ethanol for ulcer induction: one positive control (vehicle) and six with VC, VE, or GSE (25 and 250 mg/kg). Ulcer indexes and gastric levels of malondialdehyde (MDA) were quantified. VC, VE, and GSE (25 and 250 mg/kg) decreased aspirin, and ethanol-induced ulcers and MDA values compared with positive control group. The magnitude of aspirin ulcer reduction was comparable for all treatments, and MDA decrease with GSE was higher than with VC and tended to be greater, albeit none significantly, than with VE. GSE was more effective than VC and VE for lowering the ethanol ulcers, while the decrease of MDA levels with GSE was greater than with VC, but comparable to that achieved with VE. GSE protected against ethanol-induced gastric ulcers more effectively than VC or VE, while its protection against aspirin ulcers was comparable for all treatments. GSE produced the greatest reductions of gastric MDA in both models.

scholarly journals Sucrose Permeability: A Simple Non-Invasive Marker of Gastro-Duodenal Damage in Dyspepsia

2015 ◽  
Vol 13 (2) ◽  
pp. 1-5
Author(s):  
Tapan Kumar Saha Kumar Saha ◽  
Arabinda Mohan Bhattarai ◽  
Manushree Sharma
Keyword(s):  
Duodenal Ulcer ◽  
Sensitivity And Specificity ◽  
Mucosal Damage ◽  
Gastric Ulcers ◽  
Control Group ◽  
P Value ◽  
Permeability Test ◽  
Non Invasive ◽  
Endoscopic Findings ◽  
The Mean

Introduction: Diagnosis of gastro-duodenal mucosal damage usually requires endoscopy, an invasive procedure. An attempt has been made to assess the clinical validity of sucrose permeability test as a non-invasive marker of gastro-duodenal damage.Methods: The test was carried out in 65 patients of dyspepsia evaluated by endoscopy and 21 healthy controls. On endoscopy, gastro-duodenal epithelial damage was assessed in all patients of dyspepsia and grouped as: group 1 (normal gastro-duodenal mucosa, n=18), group 2 (Gastric ulcer, n=15), group 3 (Duodenal ulcer, n=10), group 4 (Gastritis, n=12) and group 5 (Duodenitis, n=10).Results: The mean urinary sucrose excretions (mg) in 5 hours after 100g oral load was found to be significantly higher in patients of gastric ulcers (183.6 ±169.7, p value < 0.001), duodenal ulcers (151.7± 79.2, p value <0.001), gastritis (115.6±59.2, p value <0.001) and duodenitis (105.2± 122.2, p value <0.05) as compared to controls (35.2±17.2). However, the mean sucrose excretion in patients of dyspepsia with normal endoscopic findings (42.5±14.8 mg) was almost similar to the control group (p value >0.05). A ROC curve analysis taking endoscopic findings as gold standard was undertaken to find the optimum cut-off to detect gastro-duodenal epithelial damage.Conclusions: The cut-off value of 64.5 mg/5h for urinary sucrose excretion provided the best sensitivity and specificity in the diagnosis of both surgical (gastric and duodenal ulcer) and non-surgical (gastritis and duodenitis) gastro-duodenal mucosal damage. At this cutoff sucrose permeability test provided a sensitivity of 72 % and specificity of 92%. Similarly, a cut-off value of 66.5 mg/5h of urinary sucrose excretion provided the best sensitivity and specificity in the diagnosis of surgical gastro-duodenal mucosal damage with sensitivity of 72% and specificity of 97%. 

scholarly journals Effect of Trianthema triquetra Rottl. ex Willed (Aizoaceae) on ethanol-induced gastric ulcers in experimental rats

2021 ◽  
Author(s):  
Hira Ijaz ◽  
◽  
Saiqa Ishtiaq ◽  
Faryal Rubab ◽  
Ans Munir ◽  
...  
Keyword(s):  
Gastric Ulcer ◽  
Disease Control ◽  
Gastric Wall ◽  
Gastric Volume ◽  
Gastric Ph ◽  
Control Group ◽  
Treated Animal ◽  
Total Acidity ◽  
Ulcer Index ◽  
Standard Drug

Trianthema triquetra Rottl. Ex.Willed (T. triquetra) is a medicinal plant that belongs to the family Aizoaceae. The plant has been used traditionally as fodder, as a remedy for chronic ulcer, fever, and healing wounds. Therefore, the present study was intended to investigate the anti-ulcer ability of different fractions of T. triquetra to verify its folklore use in ulcer cure. Acute oral toxicity of all the fractions of T. triquetra was evaluated at a dose of 2g/kg b.wt. Anti-ulcer potential of nbutanol (TTB), chloroform (TTC), ethyl acetate (TTEA) and aqueous (TTA) fraction of crude methanolic extract of T. triquetra was assessed by using ethanol- induced gastric ulcer model in rats. Omeprazole at a dose of 20 mg/kg b.wt. was used as standard drug. After 1 hour of administration of all the fractions of T. triquetra, at a dosage of 300mg/kg b.wt., the gastric ulcer was induced in all animals by administering absolute ethanol (1mL/animal) orally except normal control group. After an hour, all the rats were sacrificed. Ulcer index, % age of ulcer inhibition, gastric pH, gastric volume, total acidity, gastric wall protein, gastric wall mucus and histopathology of the stomach wall of rats were assessed. All fractions of T. triquetra showed a substantial decrease in ulcer index and improvement in percentage inhibition compared to the disease control group. There was a rise in the amount of gastric wall mucus content, total protein content, gastric pH and a decrease in gastric volume and total acidity. Histopathological studies showed severe mucosal injury, leucocyte infiltration and edema in the disease control group compared to omeprazole and plant fractions treated animal groups. The present work encourages the conventional use of T. triquetra in the cure of ulcers.

scholarly journals (-)-Carveol Prevents Gastric Ulcers via Cytoprotective, Antioxidant, Antisecretory and Immunoregulatory Mechanisms in Animal Models

2021 ◽  
Vol 12 ◽  
Author(s):  
Catarina Alves de Lima Serafim ◽  
Maria Elaine Cristina Araruna ◽  
Edvaldo Balbino Alves Júnior ◽  
Leiliane Macena Oliveira Silva ◽  
Alessa Oliveira Silva ◽  
...  
Keyword(s):  
Gastric Ulcer ◽  
Animal Models ◽  
Gastric Secretion ◽  
Gastric Wall ◽  
Lethal Dose ◽  
Mechanisms Of Action ◽  
Gastric Ulcers ◽  
Ethanol Stress ◽  
Gastroprotective Activity ◽  
Pylorus Ligation

Background: (-)-Carveol (p-Mentha-6,8-dien-2-ol) is a monocyclic monoterpenic alcohol, present in essential oils of plant species such as Cymbopogon giganteus, Illicium pachyphyllum and in spices such as Carum carvi (cumin). Pharmacological studies report its antitumor, antimicrobial, neuroprotective, vasorelaxant, antioxidant and anti-inflammatory activity.Hypothesis/Purpose: The objective of this study was to evaluate the acute non-clinical oral toxicity, gastroprotective activity of monoterpene (-)-Carveol in animal models and the related mechanisms of action.Methods: Acute toxicity was assessed according to OECD guide 423 in mice. Ethanol, stress, NSAIDs and pylorus ligation-induced gastric ulcer models were used to investigate antiulcer properties. The related mechanisms of action were using the ethanol-gastric lesions protocol.Results: (-)-Carveol has low toxicity, with a lethal dose 50% (LD50) equal to or greater than 2,500 mg/kg according to OECD guide nº 423. In all gastric ulcer induction methods evaluated, (-)-Carveol (25, 50, 100 and 200 mg/kg, p.o.) significantly reduced the ulcerative lesion in comparison with the respective control groups. To investigate the mechanisms involved in the gastroprotective activity, the antisecretory or neutralizing of gastric secretion, cytoprotective, antioxidant and immunoregulatory effects were evaluated. In the experimental protocol of pylorus ligation-induced gastric ulcer, (-)-Carveol (100 mg/kg) reduced (p &lt; 0.001) the volume of gastric secretion in both routes (oral and intraduodenal). The previous administration of blockers NEM (sulfhydryl groups blocker), L-NAME (nitric oxide synthesis inhibitor), glibenclamide (KATP channel blocker) and indomethacin (cyclo-oxygenase inhibitor), significantly reduced the gastroprotection exercised by (-)-Carveol, suggesting the participation of these pathways in its gastroprotective activity. In addition, treatment with (-)-Carveol (100 mg/kg) increased (p &lt; 0.001) mucus adhered to the gastric wall. Treatment also increased (p &lt; 0.001) levels of reduced glutathione (GSH), superoxide dismutase (SOD) and interleukin-10 (IL-10). It also reduced (p &lt; 0.001) malondialdehyde (MDA), myeloperoxidase (MPO), interleukin-1 beta (IL-1β) and tumor necrosis factor-alpha (TNF-α) levels.Conclusion: Thus, it is possible to infer that (-)-Carveol presents gastroprotective activity related to antisecretory, cytoprotective, antioxidant and immunomodulatory mechanisms.

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