scholarly journals PREPARATIVE ISOLATION OF SPECIFIC ANTIGENS OF FULL CELL LYSATE M. BOVIS

2021 ◽  
Vol 248 (4) ◽  
pp. 163-167
Author(s):  
A.V. Moskvicheva ◽  
◽  
K.S. Khaertynov ◽  
N.I. Khammadov ◽  
E.A. Shuralev ◽  
...  
Keyword(s):  
Food Industry ◽  
Preventive Measures ◽  
Animal Husbandry ◽  
High Sensitivity ◽  
Antigenic Structure ◽  
Economic Losses ◽  
Serological Tests ◽  
Immune Deficiencies ◽  
Specific Antigens ◽  
High Degree

Bovine tuberculosis is a serious problem for agriculture all over the world and causes huge economic losses. At the same time, infections caused by Mycobacterium bovis not only affect animal husbandry and the food industry, but also pose a threat to public health, as M. bovis is often isolated as a pathogen of tuberculosis, especially in patients with various immune deficiencies. The situation is aggravated by the lack of simple and accessible methods for diagnosing tuberculosis in cattle, which significantly impedes the monitoring of infection and the application of appropriate preventive measures. Serological tests, due to their relatively cheap cost, rapidity, rather high sensitivity and specificity, are able to compete with the allergic test during mass examinations of cows. In the present study, we present the results on the study of the antigenic structure of mycobacteria and the isolation of the most specific antigenic components from the lysate of the M. Bovis cell wall destroyed on the Fast Prep 24 device. The developed method allows obtaining a serologically active antigenic fraction with a high degree of purity with a molecular weight of 28 kDa.

scholarly journals SCHISTOSOMIASIS OF ANIMALS IN ECOLOGICAL CONDITIONS OF THE ARAL SEA REGION

2020 ◽  
pp. 313-318
Author(s):  
Norkobilov ◽  
Akramova ◽  
Shakarbayev ◽  
Azimov
Keyword(s):  
Preventive Measures ◽  
Infection Intensity ◽  
Sharp Decrease ◽  
Animal Husbandry ◽  
Aral Sea ◽  
Farm Animals ◽  
Ecological Conditions ◽  
Current State ◽  
High Degree ◽  
Spontaneous Infection

The problem of animal schistosomiasis is multifaceted. It intertwines veterinary, health, social and economic issues. The current state of the epizootology of schistosomiasis in the ecological conditions of the Aral Sea region is presented. Spontaneous infection of farm animals with mature Schistosoma turkestanica was 55.2% in cattle, sheep – 33.0%, horses – 21.5%, goats – 15.5% and camels – 10.2%. At individual farms of the Bozatau, Kungradsky, Muinaksky, Amudarya and other districts, cattle infection reached up to 100% with a high degree of infection intensity. We noted the cyclical nature of the epizootic of schistosomiasis in cattle in the Aral Sea region. The alternation of cyclicity is observed at specified intervals – from 15 to 20 years. The ups and downs in the infection intensity alternate with a certain accuracy, according to which epizootics of schistosomiasis can be predicted for several years in advance. Currently, science has developed the necessary prerequisites for a sharp decrease in the incidence of animals with schistosomiasis and the elimination of invasion in individual foci. This is achieved by a complex of therapeutic and preventive measures, as well as the use of progressive methods and technology of animal husbandry

scholarly journals Analytical Validation and Clinical Application of Rapid Serological Tests for SARS-CoV-2 Suitable for Large-Scale Screening

Diagnostics ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 869
Author(s):  
Amedeo De Nicolò ◽  
Valeria Avataneo ◽  
Jessica Cusato ◽  
Alice Palermiti ◽  
Jacopo Mula ◽  
...  
Keyword(s):  
Early Diagnosis ◽  
Real Time Pcr ◽  
Large Scale ◽  
High Sensitivity ◽  
Analytical Validation ◽  
Pcr Assay ◽  
Serological Tests ◽  
Flow Test ◽  
Lateral Flow Test ◽  
Large Scale Screening

Recently, large-scale screening for COVID-19 has presented a major challenge, limiting timely countermeasures. Therefore, the application of suitable rapid serological tests could provide useful information, however, little evidence regarding their robustness is currently available. In this work, we evaluated and compared the analytical performance of a rapid lateral-flow test (LFA) and a fast semiquantitative fluorescent immunoassay (FIA) for anti-nucleocapsid (anti-NC) antibodies, with the reverse transcriptase real-time PCR assay as the reference. In 222 patients, LFA showed poor sensitivity (55.9%) within two weeks from PCR, while later testing was more reliable (sensitivity of 85.7% and specificity of 93.1%). Moreover, in a subset of 100 patients, FIA showed high sensitivity (89.1%) and specificity (94.1%) after two weeks from PCR. The coupled application for the screening of 183 patients showed satisfactory concordance (K = 0.858). In conclusion, rapid serological tests were largely not useful for early diagnosis, but they showed good performance in later stages of infection. These could be useful for back-tracing and/or to identify potentially immune subjects.

scholarly journals Poult Enteritis and Mortality Syndrome in Turkey Poults: Causes, Diagnosis and Preventive Measures

Animals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 2063
Author(s):  
Awad A. Shehata ◽  
Shereen Basiouni ◽  
Reinhard Sting ◽  
Valerij Akimkin ◽  
Marc Hoferer ◽  
...  
Keyword(s):  
Preventive Measures ◽  
Clinical Signs ◽  
Accurate Diagnosis ◽  
Economic Losses ◽  
Turkey Poults ◽  
Causative Agents ◽  
Enteric Disorders ◽  
And Control ◽  
Enteropathogenic Viruses ◽  
Poult Enteritis

Poult enteritis and mortality syndrome (PEMS) is one of the most significant problem affecting turkeys and continues to cause severe economic losses worldwide. Although the specific causes of PEMS remains unknown, this syndrome might involve an interaction between several causative agents such as enteropathogenic viruses (coronaviruses, rotavirus, astroviruses and adenoviruses) and bacteria and protozoa. Non-infectious causes such as feed and management are also interconnected factors. However, it is difficult to determine the specific cause of enteric disorders under field conditions. Additionally, similarities of clinical signs and lesions hamper the accurate diagnosis. The purpose of the present review is to discuss in detail the main viral possible causative agents of PEMS and challenges in diagnosis and control.

scholarly journals Interference studies of diamond faces. A crossed fringe technique

1947 ◽  
Vol 191 (1025) ◽  
pp. 182-194 ◽  
Keyword(s):  
Crystal Surface ◽  
High Sensitivity ◽  
Multiple Beam ◽  
Solution Mechanism ◽  
High Degree ◽  
Interference Studies

Precision multiple-beam interference methods are applied to the study of the topography of octahedral faces of three diamonds. A new crossed fringe technique of high sensitivity is described. Evidence is adduced to support the view that the curvature of diamond faces and the occurrence of triangular pits are to be attributed to growth and not to solution. The observations thus settle a long-standing controversy. Irregular depressions which are observed are attributed to a solution mechanism. New features associated with the triangular growth pits are described and interpreted. A crystal surface with regions showing an abnormally high degree of optical plane uniformity is described. The surface of a polished diamond is described.

PRACTICAL SOLUTIONS FOR IMPLEMENTATION ON ENTERPRISES OF THE FOOD INDUSTRY OF THE SYSTEM FOOD SAFETY MANAGEMENT

2020 ◽  
Author(s):  
Н.В. АГЕЕВА ◽  
В.К. КОЧЕТОВ ◽  
Е.Ю. ЛИТВИНЕНКО
Keyword(s):  
Food Safety ◽  
Management System ◽  
Food Industry ◽  
Preventive Measures ◽  
Safety Management ◽  
Control Points ◽  
Quality And Safety ◽  
Critical Control Points ◽  
Physical Chemical ◽  
Confectionery Products

Рассмотрен опыт внедрения системы менеджмента безопасности пищевой продукции на ОАО Кондитерский комбинат «Кубань». Установлены физические, химические и микробиологические факторы, снижающие безопасность продукции, производимой на ОАО Кондитерский комбинат «Кубань», – мучных кондитерских изделий и продукции цеха шоколадного производства. Перечислены разработанные и внедренные на комбинате пререквизитные программы для предупреждения опасности загрязнения продукции. Установлено, что внедрение превентивных мер позволило: поэтапно сократить количество критических контрольных точек на комбинате с 88 до 4, обеспечить отсутствие рекламации по качеству и безопасности выпускаемой продукции от контролирующих органов, снизить в 2019 г количество претензий от потребителей на 10%. по сравнению с 2018 г. The experience of implementing the food safety management system at OJSC Kuban Confectionery plant is shown. Physical, chemical and microbiological factors that reduce the safety of products produced at the Kuban Confectionery plant-flour confectionery products and products of the chocolate production workshop, have been established. Preliminary programs developed and implemented at the plant to prevent the risk of contamination of products are listed. It was found that the introduction of preventive measures allowed: to gradually reduce the number of critical control points at the plant from 88 to 4, to ensure that there are no complaints about the quality and safety of products from regulatory authorities, to reduce by 10% in 2019 the number of claims from consumers compared to 2018.

Markers of the early stage of rheumatoid arthritis

2016 ◽  
Vol 51 (4) ◽  
pp. 305-314
Author(s):  
Beata Polińska ◽  
Joanna Matowicka-Karna ◽  
Halina Kemona
Keyword(s):  
Rheumatoid Arthritis ◽  
Human Population ◽  
Early Stage ◽  
Clinical Signs ◽  
High Sensitivity ◽  
Unknown Etiology ◽  
Serological Tests ◽  
Citrullinated Peptide ◽  
Peptide Antibodies

Rheumatoid arthritis (RA) is a chronic, autoimmune connective tissue disease of unknown etiology. RA affects about 1% of the human population, women suffer three times more often than men, with the peak incidence between the age of 40 to 50. The up-to-date criteria from 2010 for the diagnosis of RA include: occurrence and duration of clinical signs, indicators of inflammation and serological tests. Neopterin, a protein released by macrophages, is a sensitive indicator of inflammation and the severity of RA. Regarding the serological tests, anti-cyclic citrullinated peptide antibodies represent a well-known marker with the specificity for RA of about 98%. The antibodies may be present in the serum of patients even a few years before the first clinical signs of the disease, heralding erosive changes in the joints and more severe course of RA. The literature also contains reports about autoantibodies anti-CarP and anti-Sa/ anti-MCV, which may occur in people with pain and swelling of joints and precede full-blown development of RA as well as reflect disease activity. Serological diagnosis of RA may be supported by some genetic tests based on PCR for detecting mutations e.g. C1858T in the PNPN22 gene. In turn, the quantitative analysis of different classes of miRNAs seems justified in order to better classify patients showing symptoms of RA. Further studies are needed that take into account the role of different markers in the development of RA, and confirm the high sensitivity and specificity of these markers in the diagnosis of the disease.

Textile Museum Collections. SIM Method Validation for the Assessment of Pesticides

2021 ◽  
Vol 58 (3) ◽  
pp. 1-10
Author(s):  
Elena-Cornelia Mitran ◽  
Irina-Mariana Sandulache ◽  
Cristina-Mihaela Lite ◽  
Lucian Gabriel Radu
Keyword(s):  
Analytical Techniques ◽  
High Sensitivity ◽  
Pest Infestation ◽  
Accuracy And Precision ◽  
Cis And Trans Isomers ◽  
Quantitative Results ◽  
Cis And Trans ◽  
High Degree ◽  
Non Destructive ◽  
Detection And Quantification

In time the environmental conditions could damage textiles (materials/ artifacts) causing the need to develop better non-destructive or at least micro-destructive analysis techniques of the samples. There are ethnographic textile artifacts that were treated in the past with various pesticides, that have not been mentioned in any document. These are often re-treated with chemicals by museum staff as a method of preventing pest infestation. Due to the progressive use of many pesticides, this paper was focused on the detection and quantification of three pesticides: malathion, methoxychlor, and permethrin (cis- and trans- isomers). Gas chromatography is one of the most widely used analytical techniques for characterizing volatile organic compounds and therefore was the analytical method of choice for the present study. Because these analytes are found at trace levels, the detection and quantification limits of analytes are very small and it is necessary to optimize and validate a SIM method - that allows the mass spectrometer to detect specific compounds with high sensitivity. In SIM mode, the instrument is set to collect data at selected masses of interest, thus increasing the accuracy and precision of the quantitative results. The present paper is aimed to develop this type of method with specificity and selectivity, high precision (expressed in terms of repeatability and intermediate accuracy), accuracy, suitable working range and linearity, and high degree of series� homogenity.

scholarly journals Seroprevalance of Toxoplasmosis in sheep and goat: Iraq/ Sulaimania

2011 ◽  
Vol 35 (1) ◽  
pp. 16-24
Author(s):  
Lazem H. Al-Taie
Keyword(s):  
Age Groups ◽  
Neural System ◽  
Farm Animals ◽  
Economic Losses ◽  
Age Group ◽  
Serum Samples ◽  
Serological Tests ◽  
Back Ground ◽  
Qualitative Determination

Back ground: Toxoplasmosis is an important zoonosis that causes economic losses in animal herds due to abortion and stillbirth as well as changes in the reproductive and neural system of susceptible animals . Objective: The aims of the present study is to determination the prevalence of T. gondii in farm animals ( sheep& goat)of both genders and different ages in Sulaimani province by using two serological tests (ELISA and LAT). Methods: Blood samples were collected from farm animals ,142 sheep and 46 goats , of different sexes and ages. Tow different serological tests ,ELISA and LAT for qualitative determination of T. gondii antibody titer in sheep and goats serum samples. Results: The prevalence rate in sheep was 73 (51.7 %) and 82 (57 %) , and 21 (54.6 %) and 25 (54.35 %) in goats ,by ELISA and LAT respectively. The prevalence of toxoplasmosis was highest in age group 7-9 (66.6%) in sheep in compares’ with other age groups. There was no significant differences between both spp.and tow test. Conclusion: Statistical results show no significant differences between both tests (ELISA &LAT) at (P ≥ 0.05).The prevalence of toxoplasmosis was increased proportionally with the age of animals, while gender has no effect on the prevalent rate .

scholarly journals The Mechanism Underlying the Extreme Sensitivity of Duck to Aflatoxin B1

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Kuntan Wu ◽  
Minjie Liu ◽  
Huanbin Wang ◽  
Shahid Ali Rajput ◽  
Yajing Shan ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Aflatoxin B1 ◽  
High Sensitivity ◽  
Metabolic Activation ◽  
Cytochrome P450s ◽  
Economic Losses ◽  
Metabolic Detoxification ◽  
Glutathione S Transferases ◽  
Extreme Sensitivity ◽  
Mutual Conversion

Most metabolites of aflatoxin B1 (AFB1), especially exo-AFB1-8,9-epoxide (AFBO), can induce the production of reactive oxygen species (ROS) to vary degrees, causing oxidative stress and liver damage, and ultimately induce liver cancer in humans and animals. Duck is one of the most sensitive animals to AFB1, and severe economic losses are caused by duck AFB1 poisoning every year, but the exact mechanism of this high sensitivity is still unclear. This review highlights significant advances in our understanding of the AFB1 metabolic activation, like cytochrome P450s (CYPs), and AFB1 metabolic detoxification, like glutathione S-transferases (GSTs) in poultry. In addition, AFB1 may have other metabolic pathways in poultry, such as the mutual conversion of AFB1 and aflatoxicol (AFL) and the process of AFBO to produce AFB1-8,9-dihydrodiol (AFB1-dhd) and further metabolize it into detoxification substances. This review also summarized some exogenous regulatory substances that can alleviate AFB1-induced oxidative stress.

Impedance Screening for Preschool Children: State of the Art

1979 ◽  
Vol 88 (1) ◽  
pp. 56-65 ◽  
Author(s):  
Jack L. Paradise ◽  
Clyde G. Smith
Keyword(s):  
Preschool Children ◽  
Middle Ear ◽  
Large Scale ◽  
High Sensitivity ◽  
Cutoff Point ◽  
Screening Programs ◽  
Large Numbers ◽  
Middle Ear Disease ◽  
High Degree ◽  
Large Scale Screening

As a test for detecting middle ear disease among preschool children, tympanometry — as opposed to audiometry — has three advantageous attributes: a high degree of sensitivity, minimal need for subject cooperation, and total objectivity. For these reasons interest has arisen in tympanometry as a method for screening, i.e., identifying children with previously undetected middle ear disease. However, uncertainty persists concerning the importance of detecting apparently asymptomatic middle ear effusions, and concerning optimal methods, or even the advisability, of treating them. Further, the sensitivity and specificity of tympanometry depend on how the pass-fail cutoff point is defined. Defining this cutoff point so as to achieve high sensitivity may result in excessively low specificity, with the production of large numbers of false-positives who then become overreferrals. Data are presented to show how the validity of the test may be increased to some extent by attention to the gradient of “negative-pressure” tympanograms. At the present time, given the various aforementioned uncertainties, and with adequate validation as to the presence or absence of disease often lacking in reported studies of impedance screening in preschool populations, the cumulative results of these studies do not warrant embarking on large-scale screening programs. What is needed instead is additional research to explore the issue further.

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