scholarly journals Influence of heat stress on the quality of sperm production of boars-producers of different breeds and its fertilizing ability

2021 ◽  
pp. 106-114
Author(s):  
M. P. Gutman ◽  
N. N. Gorb ◽  
V. M. Sorokoletova
Keyword(s):  
Heat Stress ◽  
Semen Quality ◽  
Embryo Implantation ◽  
Sperm Concentration ◽  
The Body ◽  
Sperm Production ◽  
Large White ◽  
Study Results ◽  
Fertilizing Ability

The primary purpose of this work is to study the heat stress on the quality of sperm production in boars of different breeds (Large White, Landrace, Duroc, Maxgro). The authors found that heat stress affects the activity of pigs. The desire to reduce heat production leads to sedentary, passive animals, accompanied by decreased appetite, shortness of breath and inhibition of sexual reflexes in individuals. Against this background, the disturbances arising in the body, reinforced by a local increase in scrotal temperature, harm semen quality. The authors observed a tendency for decreased ejaculate volume and a decrease (statistically significant in most breeds) in the sperm concentration in the ejaculate by 11.47-34.96%. But at the same time, the organoleptic values (colour, consistency, smell) did not change. Heat stress hurts the fertility of pigs, reducing the probability of successful embryo implantation. The fertility of sow inseminated with semen from boars during heat stress decreased by 10.3-23.7%. And also, the fertility of the sow did not reach pre-stress levels until the end of the observation. Based on the study results, the authors found that, depending on the breed, pigs can tolerate heat stress in a more severe or milder form. The species Large White and Maxgro are less sensitive to increased ambient temperature (they had fewer changes in general condition, semen and fertility) than Duroc and Landrace pigs.

scholarly journals Effect of Heat Stress on Buck’s Adaptability and Semen Characteristics

2016 ◽  
Vol 9 (1) ◽  
pp. 151-156 ◽  
Author(s):  
A Rahman ◽  
MM Hossain ◽  
M Khan ◽  
MT Kamal ◽  
MA Hashem
Keyword(s):  
Heat Stress ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Sun Exposure ◽  
Biological Data ◽  
Measured Temperature ◽  
Semen Characteristics ◽  
Humidity Index ◽  
Severe Heat Stress

The experiment was conducted to investigate the effect of heat stress on adaptability and semen quality of buck. Almost similar 10 Black Bengal bucks were selected for the study at Artificial Insemination Centre, Bangladesh Agricultural University, Bangladesh. Prerequisite biological data were collected from each animal before keeping them sun heat and after 1 hour sun exposure. Different semen characteristics of each animal were measured. Temperature humidity index (THI) was 19.95 in the morning which indicate all the animals were in absence of heat stress and THI was 25.54 in the noon which indicate all the animals were in severe heat stress at noon. Buck 1 and buck 5 were more heat resistant (p<0.01), because their thermolysis capacity (TC) values were less than the others. There were no differences in tunica dartos index and semen characteristics among different bucks (p>0.05). Positive correlation between sperm motility and testis length (p<0.05), and negative correlation between sperm concentration and scrotal skin temperature (p<0.05) were found. From the study it can be concluded that all the bucks were in severe heat stress at noon time and TC can be used as an indicator for selection of buck for heat tolerance.J. Environ. Sci. & Natural Resources, 9(1): 151-156 2016

scholarly journals REPRODUCING QUALITIES OF RAMS OF THE MOLDOVAN TYPE KARAKUL BREED

2020 ◽  
Vol 59 ◽  
pp. 92-96
Author(s):  
D. Rotari
Keyword(s):  
Sperm Count ◽  
Sperm Concentration ◽  
Animal Husbandry ◽  
Sperm Production ◽  
Livestock Farming ◽  
High Quality ◽  
Average Percentage ◽  
Reproductive Ability ◽  
First Time

Issues related to the reproduction of animals have been and remain one of the most complex and relevant problems of biology and are constantly finding a direct and effective way out into livestock farming practice. The rational use of breeding sheep as producers is limited by the lack of standard, objective methods and methods for the timely assessment of their reproductive ability. The article presents the results of evaluating the sperm production of rams-producers. For the first time, the freshly obtained sperm of rams of Moldavian type producers of the Karakul breed was evaluated using the macroscopic method - ejaculate volume, color and smell, as well as the microscopic method - motility, sperm concentration in the ejaculate, total sperm count in the ejaculate sperm movements (VAP; VSL and VCL) as well as the percentage of abnormal forms of sperm. The experiments were carried out on sheep producers grown on a pedigree farm of the experimental farm of the Moldavian Scientific and Practical Institute of Biotechnology in Animal Husbandry and Veterinary Medicine. As a result of the studies, it was found that the ejaculate volume on average was 0.99 ± 0.04 ml, motility was 0.95 ± 0.02 and sperm concentration 1.51 ± 0.14 billion/ml. The percentage of pathological forms in sperm averaged 13.72 ± 0.61, an indicator that characterizes the high quality of sperm. The average quality indicator of ejaculates obtained from ram-producers of the Moldavian type of the Karakul breed corresponds to physiological standards for the Karakul breed. The average percentage of pathological forms of sperm found in ejaculates indicates that the rams were in good conditions of feeding and keeping. According to research, we can say that the Moldovan type of Karakul rams can be successfully used to obtain high quality ejaculates suitable for freezing sperm at 196°C.

scholarly journals Assessment of quality in specific fractions of Large White Yorkshire boar semen

2021 ◽  
Vol 52 (2) ◽  
Author(s):  
K. G. Ambily ◽  
Malati Naik ◽  
Hiron M. Harshan ◽  
C. Jayakumar ◽  
M. P. Unnikrishnan ◽  
...  
Keyword(s):  
Cold Shock ◽  
Sperm Concentration ◽  
Membrane Integrity ◽  
Membrane Cholesterol ◽  
Large White ◽  
Progressive Motility ◽  
Sperm Membrane ◽  
Boar Semen ◽  
Quality Assessments

Boar semen is voluminous and ejaculated as jets or fractions of pre-sperm, sperm rich (SRF) and post-sperm rich fractions. Recent studies have reported more resilient characteristics of sperm in initial portions of SRF towards cold shock and cryopreservation. The present study was conducted to assess the quality of specific fractions of SRF, namely, first 10mL of SRF (F1) and rest of SRF (F2) in Large white Yorkshire (LWY) boar semen. Ejaculates were collected using gloved-hand technique and were subjected to quality assessments of volume, pH, sperm progressive motility, concentration, plasma membrane integrity, abnormality, acrosome integrity and sperm membrane cholesterol. Upon statistical analysis, significant differences were noticed in volume, pH, sperm concentration and sperm membrane cholesterol between fractions of the ejaculate.

scholarly journals Semen quality and diversity of morphological sperm abnormalities in bulls: breed and strain effects

2019 ◽  
Vol 22 (8) ◽  
pp. 931-938
Author(s):  
M. A. Kleshchev ◽  
V. L. Petukhov ◽  
L. V. Osadchuk
Keyword(s):  
Sperm Motility ◽  
Environmental Conditions ◽  
Western Siberia ◽  
Semen Quality ◽  
Sperm Count ◽  
Sperm Concentration ◽  
Sperm Head ◽  
Sperm Dna Fragmentation ◽  
Sperm Production ◽  
Sperm Abnormalities

At present great attention is paid to studying genetic regulation of farm animal adaptations to environmental conditions. This problem is very important due to a wide expansion of highly productive cattle breeds created in Europe and North America. However, until the present no investigation of changing semen quality in bulls of imported breeds during their adaptations to environmental conditions of Western Siberia has been conducted. The aim of this study was to investigate semen quality peculiarities and the diversity of morphological sperm abnormalities in bulls of imported and local breeds kept in the environmental conditions of the southern part of Western Siberia. We determined sperm concentration, sperm count, and rate of sperm with progressive motility and percentage of morphologically normal spermatozoa. The rate of sperm abnormalities according to Blome’s classifcation was determined too. It was found that the mean values of sperm concentration, sperm motility and percentage of morphologically normal spermatozoa in the bulls investigated were similar to those in bulls kept in European countries. Inter­breed differences in these parameters were not found. However, bulls of the Red Danish, Angler, and Simmental breeds had a higher percentage of misshapen sperm head and pyriform sperm head than bulls of the Black­White breed. An inter­strain difference in sperm motility in bulls of the Black­White breed was observed. It was found that bulls of Reflection Sovereign 198998 strain had lover sperm motility than bulls of Wis Burke Ideal 1013415 strain. No inter­strain differences in sperm production, percentage of morphologically normal spermatozoa and rate of main sperm abnormalities were found. Thus, it has been found that the environmental conditions of the southern part of Western Siberia do not seriously affect the sperm production, sperm motility or percentage of morphologically normal spermatozoa in bulls. However, the increased rate of misshapen and pyriform sperm heads in the bulls of the foreign breeds points to a need to study sperm DNA fragmentation.

Factors affecting the response of working boars to changing environmental temperature

1991 ◽  
Vol 1991 ◽  
pp. 140-140
Author(s):  
Angela H Cliff ◽  
J H Dunne ◽  
P R English ◽  
J S M Hutchinson ◽  
O Macpherson
Keyword(s):  
Heat Stress ◽  
Adverse Effects ◽  
Environmental Temperature ◽  
Semen Quality ◽  
Sperm Quality ◽  
Sperm Concentration ◽  
Factors Affecting ◽  
High Environmental Temperature ◽  
Sudden Rise ◽  
Sperm Output

It is well established that high environmental temperature can have adverse effects on sperm concentration and the normality of sperm in the boar. Induced abnormalities include reduced motility, abnormal heads, proximal droplets, coiled and bent tails and abnormal acrosomes (Malmgren and Larsson, 1989). While all stages of spermatogenesis can be adversely affected, the primary spermatocytes are most vulnerable to these influences. Since spermatogenesis occurs over 45 days, any adverse effects of elevated ambient temperature can affect sperm quality for around 6 weeks. The maximum period of infertility appears around weeks 3 and 4 after heat stress. Reduced levels of testosterone and sometimes of LH following heat stress are implicated in these adverse effects. There is evidence that boars subjected to high constant temperature (30°C), which have become acclimatised to such, are affected less in terms of sperm output and quality (Cameron and Blackshaw, 1980) than when boars are subject to sudden major increases in temperature from fairly low levels (15 to 30 C) e.g. during the summer months (Antonyuk et al, 1983). There also appears to be large differences between boars in their ability to adapt to exposure to high environmental temperature by minimising temperature rise and avoiding adverse effects on semen quality (Cameron and Blackshaw, 1980). These workers found that boars prone to heat stress show an acute rise in body temperature in response to elevated environmental temperature and this sudden rise has a more adverse effect on semen quality than the length of exposure to the elevated temperature. There appears to be little information available on the reasons for such important between boar differences. This study was carried out to determine rectal temperature responses of boars to varying summer temperature in an intensive pig enterprise in Scotland and to attempt to determine some of the factors associated with ‘high’ and ‘low’ responding boars.

23 Sperm quality of Pure Spanish stallions is affected by inbreeding coefficient and age

2020 ◽  
Vol 32 (2) ◽  
pp. 137
Author(s):  
Y. Pirosanto ◽  
M. Valera ◽  
A. Molina ◽  
J. Dorado ◽  
S. Demyda-Peyrás
Keyword(s):  
Sperm Motility ◽  
Inbreeding Depression ◽  
Negative Correlation ◽  
Semen Quality ◽  
Sperm Quality ◽  
Sperm Concentration ◽  
Inbreeding Coefficient ◽  
Semen Parameters ◽  
Computer Assisted

Inbreeding depression, a genetic condition produced by the mating of close-related individuals, has been associated with a reduction of fertility in several species. However, a loss in sperm quality was also associated with age. In horses, the few existing reports have described a tendency of both parameters to produce a negative effect on sperm quality. However, those reports were performed using a subjective evaluation of sperm motility. In the present study, a total of 692 ejaculates from 86 Pure Spanish stallions (PRE), aged between 3 and 22 years, were evaluated using a computer-assisted methodology to determine the effect of inbreeding in four semen parameters: free-gel volume (V), sperm concentration (C, by haemocytometer), and total (TM) and progressive (PM) sperm motility (by Spermvision sperm class analyser; Minitube). The inbreeding coefficient (F) was estimated using 300 000 PRE pedigree records approximately (minimum pedigree depth, eight equivalent complete generations; range, between 1 and 30.1%). Stallion, age, ejaculate, and season of semen collection were the variables included in the statistical model (general linear model), with ejaculate and season being the variables with a major effect (by variance components analysis). Our results showed that sperm concentration (r=−0.18; P&lt;0.0001) and volume (to a lesser extent) were reduced with advancing age, both showing a major decline after 15 years of age. To the contrary, sperm motility was not affected by age of the stallion. We also found a negative correlation between the inbreeding coefficient and ejaculate volume (r=−0.14; P&lt;0.001), with a marked decrease seen when F was between 7 and 20%. Also, a negative correlation was observed in PM (r=−0.08; P&lt;0.05), although to a lower extent. Conversely, C and TM were not affected by inbreeding depression (P&gt;0.05). In conclusion, our results demonstrated that high levels of inbreeding can compromise severely the sperm quality of the PRE stallion, which, subsequently, may have a negative influence on fertility. Ongoing studies using genomic data will help to detect genetic variants associated with stallion semen quality and how it is influenced by inbreeding in specific genomic regions.

scholarly journals Effect of Selenium and Vitamin E Supplementation on Semen Quality in Dogs with Lowered Fertility

2015 ◽  
Vol 59 (1) ◽  
pp. 85-90 ◽  
Author(s):  
Anna Domosławska ◽  
Sławomir Zduńczyk ◽  
Wojciech Niżański ◽  
Andrzej Jurczak ◽  
Tomasz Janowski
Keyword(s):  
Vitamin E ◽  
Semen Quality ◽  
Sperm Count ◽  
Sperm Concentration ◽  
Control Group ◽  
Organic Selenium ◽  
Healthy Dogs ◽  
Manual Manipulation ◽  
Motility Parameters

Abstract Thirty clinically healthy dogs with poor semen quality were used in the study. Fifteen dogs were supplemented daily with selenium (0.6 mg/kg organic selenium from yeast) and vitamin E (5 mg/kg) per os for 60 d. The control group (15 dogs) was not supplemented. Semen was collected from all dogs by manual manipulation on days 0, 30, 60, and 90. The sperm concentration and motility parameters were evaluated with a Hamilton Thorne sperm analyser, version IVOS 12.3. For the assessment of sperm morphology, Diff-Quik stain was used. The percentage of live and dead spermatozoa was estimated on dried smears stained with eosin-nigrosin. The concentration of spermatozoa, most motility parameters determined (PMOT, VSL, VCL, ALH, BCF, RAPID, MEDIUM, SLOW, and STATIC), and the percentage of spermatozoa morphologically normal and live increased significantly (P < 0.05) after 60 d of supplementation. In the control group, there were no changes in motility parameters while the concentration and total sperm count decreased over the duration of the study. In conclusion, supplementation with selenium and vitamin E for 60 d can improve the quality of semen in dogs with lowered fertility.

Seasonal patterns of LH, testosterone and semen quality in the Northern pintail duck (Anas acuta)

2000 ◽  
Vol 12 (4) ◽  
pp. 229 ◽  
Author(s):  
L. M. Penfold ◽  
D. E. Wildt ◽  
T. L. Herzog ◽  
W. Lynch ◽  
L. Ware ◽  
...  
Keyword(s):  
Breeding Season ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Day Length ◽  
Seasonal Patterns ◽  
Sperm Production ◽  
Normal Morphology ◽  
Northern Pintail ◽  
Sperm Trait ◽  
Percent Normal

This study characterized seasonal changes in circulating LH and testosterone and in semen production and quality in the Northern pintail duck. Plasma LH and testosterone were measured in blood samples collected weekly throughout the year from eight males exposed to natural fluctuations in day length and temperature. Semen quality was evaluated weekly in these same males from April–June, the months when spermatozoa were produced. Semen quality (based on sperm concentration and normal morphology) peaked 0–2 weeks after sperm production onset and decreased sharply before sperm production cessation in late June. Nadir LH concentrations were measured in July and August with peak LH observed in May and November. There were clear seasonal patterns in circulating testosterone with July–September values being less (P<0.05) than October–December which, in turn, were less (P<0.05) than January–March. Maximal circulating testosterone (P<0.05) occurred during April–June, coincident with semen production. Weekly circulating LH during the breeding season was directly related to testosterone concentrations (P<0.01), but was not correlated to any specific semen or sperm trait (P>0.05). Testosterone concentrations throughout the breeding season were correlated (P<0.05) to total numbers of spermatozoa produced (volume cell concentration) and percent normal sperm morphology. In summary, the Northern pintail experiences seasonal hormone fluctuations, with maximum circulating testosterone coinciding with peak ejaculate quality reflected by the production of high numbers of morphologically normal spermatozoa.

scholarly journals Semen quality of three breeds of exotic boar extended using methaloninated Moringa leaf extract as antibiotics in beltsville thawing solution

2021 ◽  
Vol 48 (1) ◽  
pp. 47-54
Author(s):  
I. A. Omodewu ◽  
T. Olorungbohunmi ◽  
B. A. Boladuro ◽  
J. O. Abiona ◽  
C. P. Njoku
Keyword(s):  
Leaf Extract ◽  
Storage Time ◽  
Moringa Oleifera ◽  
Semen Quality ◽  
Antibacterial Properties ◽  
Semen Parameters ◽  
Large White ◽  
Normal Limits ◽  
Qualité Du Sperme

Moringa oleifera leaf extract (MLE) contains antibacterial properties; this study evaluated the effect of including MLE, breed and storage time on semen quality of boars extended with Beltsville thawing solution (BTS). Semen samples were collected weekly from twelve boars (Four Duroc, four Large White and four Landrace). Samples were extended with 0, 0.25, 0.50, 0.75 and 1.00g MLE inclusions. Parameters; volume and concentration were taken immediately after collection while pH, mass activity (%MA), progressive motility (%PM), liveability (%LA) and abnormality (%ABN) were taken at 0, 24, 48 and 72 hours. Data obtained were subjected to analysis of variance. All parameters considered except %ABN were significant (p<0.05). MA ranged between 3.31 (1.00gMLE) and 4.00 (0gMLE). Highest PM was 60.93% in 0.25gMLE, pH differed with 6.98 (0gMLE) and 6.90 (1.00gMLE), 0gMLE (89.64%) had higher LA compared to 0.25gMLE (86.40%), ABN ranged between3.94 to 4.60%. As storage time increased viability of all semen parameters reduced, between 48 and 72 hours parameters reduced below normal limits. Breed effect differed (p<0.05) for MA, Volume and Conc., Duroc (3.78) had higher MA compared to Large White (3.64) and Landrace (3.60). Semen volume (p<0.05) for Landrace (255.20g) was highest while Duroc (170.80g) was lowest. Large white concentration (201.8million spermatozoa/ml) was higher (p<0.05) than Landrace (187.4 million spermatozoa/ml) and Duroc (123.8million spermatozoa/ml). %LA ranged between 88.06% and 88.72% for Duroc and landrace respectively while %ABN was between 3.97-4.41% for Large White and Landrace. This study concluded that 0.75-1.00gMLE could replace synthetic antibiotic in BTS for 24 hours.     L'extrait de feuille de Moringa oleifera (le 'MLE') contient des propriétés antibactériennes ; Cette étude a évalué l'effet de l'inclusion de 'MLE', de la race et du temps de stockage sur la qualité du sperme de verrats exotiques additionnés de solution de décongélation de Beltsville (le 'BTS'). Des échantillons de sperme ont été prises chaque semaine de douze verrats (quatre Duroc, quatre Large White et quatre Landrace). Les échantillons ont été étendus avec des inclusions de 0, 0,25, 0,50, 0,75 et 1,00 g de 'MLE'. Paramètres ; le volume et la concentration ont été prélevés immédiatement après le prélèvement tandis que le pH, l'activité massique (% 'MA'), la motilité progressive (% 'PM'), l'habitabilité (% LA) et l'anomalie (% ABN) ont été pris à 0, 24, 48 et 72 heures. Les données obtenues ont été soumises à une analyse de variance. Tous les paramètres considérés à l'exception du% ABN étaient significatifs (p <0,05). Le MA variait entre 3,31 (1,00 g MLE) et 4,00 (0 gMLE). La'PM la plus élevée était de 60,93% dans 0,25 gMLE, le pH différait de 6,98 (0 gMLE) et 6,90 (1,00 gMLE), 0 gMLE (89,64%) avait un LA plus élevé par rapport à 0,25 gMLE (86,40%), l''ABN' variait entre 3,94 et 4,60%. À mesure que la durée de stockage augmentait la viabilité de tous les paramètres du sperme, entre 48 et 72 heures, les paramètres étaient réduits en dessous des limites normales. L'effet de la race était différent (p <0,05) pour la MA, le volume et la concentration. Duroc (3,78) avait une MA plus élevée que le Large White (3,64) et le Landrace (3,60). Le volume de sperme (p <0,05) pour Landrace (255,20 g) était le plus élevé, tandis que Duroc (170,80 g) était le plus faible. La grande concentration de blanc (201,8 millions de spermatozoïdes / ml) était plus élevée (p <0,05) que Landrace (187,4 millions de spermatozoïdes / ml) et Duroc (123,8 millions de spermatozoïdes / ml). % LA variait entre 88,06% et 88,72% pour Duroc et landrace respectivement tandis que% ABN était entre 3,97-4,41% pour Large White et Landrace. Cette étude a conclu que 0,75-1,00 gMLE pouvait remplacer l'antibiotique synthétique dans le BTS pendant 24 heures.

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