Technical note: Effects of filter bags on neutral detergent fiber recovery and fiber digestion in vitro

Two types of anaerobic fungi were isolated from the rumen of a sheep fed wheat straw. The fungus producing a mycelium in culture digested significantly more organic matter, neutral detergent fiber, acid detergent fiber and cellulose from straw than the type producing a sporangium from "spherical bodies." Key words: Rumen, fungi, straw, fiber digestion

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Technical note: Precision and accuracy of in vitro digestion of neutral detergent fiber and predicted net energy of lactation content of fibrous feeds

Journal of Dairy Science ◽

10.3168/jds.2010-3098 ◽

2010 ◽

Vol 93 (10) ◽

pp. 4855-4859 ◽

Cited By ~ 24

Author(s):

M. Spanghero ◽

P. Berzaghi ◽

R. Fortina ◽

F. Masoero ◽

L. Rapetti ◽

...

Keyword(s):

In Vitro Digestion ◽

Technical Note ◽

Neutral Detergent Fiber ◽

Net Energy ◽

Precision And Accuracy

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Technical note: In vitro digestibility of amylase-treated, ash-corrected neutral detergent fiber, with addition of sodium sulfite, at 240 hours with or without rumen fluid reinoculation

Journal of Dairy Science ◽

10.3168/jds.2016-11368 ◽

2017 ◽

Vol 100 (2) ◽

pp. 1200-1202 ◽

Cited By ~ 4

Author(s):

A. Palmonari ◽

G. Canestrari ◽

E. Bonfante ◽

M. Fustini ◽

L. Mammi ◽

...

Keyword(s):

Sodium Sulfite ◽

Rumen Fluid ◽

Technical Note ◽

Neutral Detergent Fiber ◽

In Vitro Digestibility

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Comparison of in situ versus in vitro methods of fiber digestion at 120 and 288 hours to quantify the indigestible neutral detergent fiber fraction of corn silage samples

Journal of Dairy Science ◽

10.3168/jds.2015-10258 ◽

2016 ◽

Vol 99 (7) ◽

pp. 5394-5400 ◽

Cited By ~ 16

Author(s):

R.W. Bender ◽

D.E. Cook ◽

D.K. Combs

Keyword(s):

Corn Silage ◽

Neutral Detergent Fiber ◽

Fiber Digestion ◽

In Vitro Methods ◽

Fiber Fraction

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Comparison of some aspects of the in situ and in vitro methods in evaluation of neutral detergent fiber digestion

Journal of Animal Science ◽

10.2527/jas.2012-5343 ◽

2013 ◽

Vol 91 (2) ◽

pp. 838-847 ◽

Cited By ~ 9

Author(s):

S. J. Krizsan ◽

F. Jančík ◽

M. Ramin ◽

P. Huhtanen

Keyword(s):

Neutral Detergent Fiber ◽

Fiber Digestion ◽

In Vitro Methods

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USING A CELLULOLYTIC COMPLEX TO ESTIMATE IN VITRO DIGESTIBILITY OF BIRDSFOOT TREFOIL

Canadian Journal of Plant Science ◽

10.4141/cjps90-057 ◽

1990 ◽

Vol 70 (2) ◽

pp. 487-493 ◽

Cited By ~ 2

Author(s):

D. K. DAVIS ◽

R. L. McGRAW ◽

D. A. SLEPER ◽

P. R. BEUSELINCK

Keyword(s):

Dry Matter ◽

Lotus Corniculatus ◽

Neutral Detergent Fiber ◽

Birdsfoot Trefoil ◽

In Vitro Digestibility ◽

Detergent Solution ◽

Two Stage ◽

Fiber Digestion ◽

Dry Matter Digestibility

Digesting forages with a prepared cellulase solution (PCS) has been shown to be a rapid method of obtaining dry matter digestibility (DMD) estimates in many species. This procedure has not been previously used to obtain DMD estimates of broadleaf birdsfoot trefoil (Lotus corniculatus L.). A broad based population consisting of 19 accessions, representing 11 countries of origin, three germplasm pools, and two domesticated cultivars of birdsfoot trefoil was evaluated. The PCS was produced from culturing Trichoderma reesei Simmons culture number QM9414. Fiber and PCS determinations were compared to an in vitro rumen fermentation (IVDMD) procedure. The fiber components measured were; neutral detergent fiber (NDF), acid detergent fiber (ADF), and hemicellulose (HEMI). The PCS procedures involved digestion using a PCS without any pre- or post-treatment (CS), acid-pepsin digestion followed by a single-stage digestion in PCS (APCS), acid detergent solution (ADS) followed by a two-stage digestion in PCS (ADSCS), and neutral detergent solution (NDS) followed by a two-stage digestion in PCS (NDSCS). All of the fiber and PCS procedures were significantly (P ≤ 0.001) correlated with IVDMD except HEMI. The CS procedure underestimated IVDMD by 199 g kg−1; however, it had the best regression coefficient (b = 0.99), had the second best correlation coefficient (r = 0.86), and required less time than the other PCS procedures. The APCS procedure provided the best estimate of IVDMD. It overestimated the IVDMD mean of birdsfoot trefoil by only 30 g kg−1 and was the most accurate (residual standard deviation (RSD) = 22 g kg−1) approach. The CS procedure is recommended when a ranking of samples is desired and actual values of DMD are not of primary interest.Key words: Fiber digestion, germplasm, in vitro dry matter digestibility, Lotus corniculatus L., Trichodenna reesei Simmons

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Application of Selected Inoculant Producing Antifungal and Fibrinolytic Substances on Rye Silage with Different Wilting Time

Processes ◽

10.3390/pr9050879 ◽

2021 ◽

Vol 9 (5) ◽

pp. 879

Author(s):

Seong-Shin Lee ◽

Jeong-Seok Choi ◽

Dimas Hand Vidya Paradhipta ◽

Young-Ho Joo ◽

Hyuk-Jun Lee ◽

...

Keyword(s):

Lactobacillus Plantarum ◽

Dry Matter ◽

Lactobacillus Brevis ◽

Neutral Detergent Fiber ◽

Dry Matter Digestibility ◽

Fiber Digestibility ◽

Aerobic Stability ◽

Commercial Inoculant

This research was conducted to determine the effects of selected inoculant on the silage with different wilting times. The ryes were unwilted or wilted for 12 h. Each rye forage was ensiled for 100 d in quadruplicate with commercial inoculant (Lactobacillus plantarum sp.; LPT) or selected inoculant (Lactobacillus brevis 100D8 and Leuconostoc holzapfelii 5H4 at 1:1 ratio; MIX). In vitro dry matter digestibility and in vitro neutral detergent fiber digestibility were highest in the unwilted MIX silages (p < 0.05), and the concentration of ruminal acetate was increased in MIX silages (p < 0.001; 61.4% vs. 60.3%) by the increase of neutral detergent fiber digestibility. The concentration of ruminal ammonia-N was increased in wilted silages (p < 0.001; 34.8% vs. 21.1%). The yeast count was lower in the MIX silages than in the LPT silages (p < 0.05) due to a higher concentration of acetate in MIX silages (p < 0.05). Aerobic stability was highest in the wilted MIX silages (p < 0.05). In conclusion, the MIX inoculation increased aerobic stability and improved fiber digestibility. As a result of the wilting process, ammonia-N in silage decreased but ruminal ammonia-N increased. Notably, the wilted silage with applied mixed inoculant had the highest aerobic stability.

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Evaluation of the Nutrient Composition, In Vitro Fermentation Characteristics, and In Situ Degradability of Amaranthus caudatus, Amaranthus cruentus, and Amaranthus hypochondriacus in Cattle

Animals ◽

10.3390/ani11010018 ◽

2020 ◽

Vol 11 (1) ◽

pp. 18

Author(s):

Kim Margarette C. Nogoy ◽

Jia Yu ◽

Young Gyu Song ◽

Shida Li ◽

Jong-Wook Chung ◽

...

Keyword(s):

Crude Protein ◽

Dry Matter ◽

Gas Production ◽

Nutrient Composition ◽

Neutral Detergent Fiber ◽

Amaranthus Cruentus ◽

Amaranthus Caudatus ◽

Amaranthus Hypochondriacus

The amaranth plants showed high potential feed value as forage for ruminants. An in-depth study of this plant, particularly in cattle, will help extend its utilization as an alternative protein and fiber feed source in cattle feeding. In this study, the nutrient compositions of three different species of amaranth, Amaranthus caudatus L., Amaranthus cruentus L., and Amaranthus hypochondriacus L.—two varieties for each species, A.ca 74, A.ca 91, A.cu 62, A.cu 66, A. hy 30, and A. hy 48—were evaluated. The in vitro technique was used to evaluate the fermentation characteristics such as total gas production, total volatile fatty acids (VFA) concentration, pH, and ammonia concentration of the rumen fluid. Moreover, the effective degradabilities of dry matter (EDDM) and crude protein (EDCP) of the amaranth forages were determined through in situ bag technique. The amaranth forages: A. caudatus, A. cruentus, and A. hypochondriacus showed better nutritive value than the locally produced forages in Chungcheong province of Korea. The CP of the amaranth ranged from 11.95% to 14.19%, and the neutral detergent fiber (NDF) and acid detergent fiber (ADF) contents ranged from 45.53% to 70.88% and 34.17% to 49.83%, respectively. Among the amaranth varieties, A. hypochondriacus 48 showed the most excellent ruminant feed nutrient quality (CP, 14.19%; NDF, 45.53%; and ADF, 34.17%). The effective degradabilities of dry matter (EDDM; 33–56%) and crude protein EDCP (27–59%) of the amaranth were lower compared to other studies, which could be due to the maturity stage at which the forages were harvested. Nonetheless, A. hypochondriacus 48 showed the highest EDDM (56.73%) and EDCP (59.09%). The different amaranth species did not differ greatly in terms of total VFA concentration or molar proportions, total gas production, or ammonia-N concentration. The high nutrient composition, and highly effective degradability of dry matter and crude protein, coupled with the favorable fermentation characteristics, suggest that the amaranth forages showed good to excellent feed quality for cattle.

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