Intraosseous delivery of paclitaxel-loaded hydroxyapatitealginate composite beads delaying paralysis caused by metastatic spine cancer in rats

2008 ◽  
Vol 9 (5) ◽  
pp. 502-510 ◽  
Author(s):  
Tetsuya Abe ◽  
Masataka Sakane ◽  
Toshiyuki Ikoma ◽  
Mihoko Kobayashi ◽  
Satoshi Nakamura ◽  
...  
Keyword(s):  
Treatment Group ◽  
Therapeutic Effect ◽  
Hind Limb ◽  
Systemic Treatment ◽  
Local Treatment ◽  
Free Time ◽  
Control Group ◽  
Composite Beads ◽  
Group 6 ◽  
Disease Free

Object Bone is frequently the first site and the only site of breast cancer at recurrence. Local control is important especially for metastatic spine cancer, because epidural spinal cord compression is significantly associated with the quality of life and survival of these patients. The authors have developed a local delivery system of paclitaxel in the form of hydroxyapatite-alginate composite beads. This study was conducted to clarify the therapeutic effect in a rat model of metastatic spine cancer. Methods Twenty-one rats with metastatic spine cancer were divided into 3 groups: a local treatment group (6 rats), a systemic treatment group (9 rats), and a control group (6 rats). The hind-limb motor function of the animals was monitored daily by using the Basso-Beattie-Bresnahan scale. The authors monitored the disease-free time and survival times. The log-rank test was used to define statistically significant differences between the 3 groups. Results The animals in the control group developed hind-limb paralysis at a mean of 10.8 days and died at a mean of 16.0 days. The animals treated with 2.4 wt% of paclitaxel-loaded hydroxyapatite-alginate composite beads (the local treatment group) showed a 140–150% increase in the disease-free time and survival time compared with that of the control group. Although an ~ 30-fold higher dosage of paclitaxel was administered, the therapeutic effect was not evident in the systemic treatment group. Conclusions Intraosseous delivery of paclitaxel-loaded hydroxyapatite-alginate composite beads delayed paralysis caused by metastatic spine cancer in rats. The results indicate that intraosseous chemotherapy may provide an effective local treatment of metastatic spine cancer.

Novel Local Treatment with Paclitaxel-Loaded Hydroxyapatite-Alginate Gels for Spinal Bone Metastases

2007 ◽  
Vol 330-332 ◽  
pp. 1343-1346 ◽  
Author(s):  
Tetsuya Abe ◽  
Masataka Sakane ◽  
Toshiyuki Ikoma ◽  
Toru Tonegawa ◽  
Tomonori Yoshioka ◽  
...  
Keyword(s):  
Treatment Group ◽  
Patient Survival ◽  
Local Treatment ◽  
Cord Compression ◽  
Control Group ◽  
Cancer Model ◽  
Spinal Bone Metastases ◽  
Osseous Involvement ◽  
Alginate Gels ◽  
Disease Free

The extent of osseous involvement, particularly spinal cord compression, is directly correlated with patient survival. To treat metastatic spine cancer, we have developed novel paclitaxel-loaded hydroxyapatite-alginate gels. In this study, an intraosseous spinal cancer model in rats was used to investigate the efficacy of local treatment. Ten rats were randomized into two groups, a local treatment group and a control group. Disease-free time and survival rate in the local treatment group were significantly longer in this model. (p<0.05)

Clinical and microbiological aspects of vaginal microbiota at pregnant with threat of late miscarriages during treatment with vaginal gestagens forms

2017 ◽  
pp. 68-73
Author(s):  
I.P. Polishchuk ◽  
Keyword(s):  
Pregnant Women ◽  
Therapeutic Effect ◽  
Local Treatment ◽  
Vaginal Microbiota ◽  
Control Group ◽  
Before And After ◽  
Low Efficiency ◽  
Late Miscarriage ◽  
Microbiological Aspects ◽  
Micronized Progesterone

The objective: was to examine the effectiveness of treatment of late miscarriage threat by micronized form of progesterone for 100 mg – 3 times a day in the form of gelatin pills and vaginal tablets with lactose. Patients and methods. Under our supervision there were 70 pregnant women with normocenosis of vagina (NCV) without extragenital pathology, which were not performed systemic or local treatment with antibacterial drugs in the last 4 weeks. Among them 25 pregnant women with TLSM treated by gelatin tablets of micronized progesterone (GTP) (group 1); 25 pregnant women with TLSM, treated by vaginal micronized progesterone tablets (VPT) (2nd group) and 20 healthy women with physiological pregnancy – PV (control group). The distribution of women in the group adhered to the principles of randomization. The age of examined women ranged from 19 to 32 years, most pregnant women were aged under 30 years (89.02%). General clinical examination was carried out according to the standard scheme according to the Order MH of Ukraine № 620. Results. During the research we have determined the colpocytologcal dynamics and state of vaginal microbiota in pregnant women with threatened late miscarriage with initial vaginal normocenosis before and after treatment whit vaginal forms of progesterone. Conclusion. The received results showed low efficiency of micronized progesterone gelatin dragee at threat of the late miscarriage that at small therapeutic effect has led to the development of vaginal dysbiosis in all surveyed. In contrast, the use of micronized progesterone vaginal tablets – the maximally rapid therapeutic effect without disturbance of vaginal normocenosis. Key words: the threat of a late miscarriage, vaginal micronized forms of progesterone.

scholarly journals Therapeutic effect of Xuezhitong capsule on microvascular angina

2021 ◽  
Vol 20 (9) ◽  
pp. 1991-1997
Author(s):  
Xiaomei Ma ◽  
Dehui Yang ◽  
Weichun Shen ◽  
Wei Liang ◽  
Qian Lin
Keyword(s):  
Treatment Group ◽  
Therapeutic Effect ◽  
Conventional Treatment ◽  
Density Lipoprotein ◽  
Control Group ◽  
Vascular Endothelial ◽  
Beijing City ◽  
Microvascular Angina ◽  
Tnf Α ◽  
Hs Crp

Purpose: To determine the therapeutic effect of Xuezhitong capsule in patients with microvascular angina (MVA), and its impact on vascular endothelial function.Methods: In total, 172 MVA patients treated in Beijing City Fengtai District Nanyuan Hospital from September 2017 to September 2019 were selected and randomized into control group which received conventional treatment, and treatment group which received Xuezhitong capsules plus. There were 86 patients in each group. Therapeutic effect, levels of inflammatory factors, i.e., high-sensitivity C- reactive protein (hs-CRP), interleukin-(IL-6), tumor necrosis factor-α (TNF-α), and vascular endothelial factors such as nitric oxide (NO), thromboxane B2 (TXB2) and endothelin (ET), were determined.Results: Markedly higher total treatment effectiveness was observed in the treatment group than in the control group (89.53 % vs. 72.94 %; p < 0.05). In both groups, treatment reduced the levels of hs-CRP, IL-6, TNF-α, TXB2 and ET, but elevated NO, with better results for treatment group than the control group (p < 0.05). Better optimizations of total cholesterol (TC), triglycerides (TGs), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein (HDL) were observed in the treatment group, relative to the control group (p < 0.05). Patients in the treatment group experienced fewer (8.14%) adverse reactions than those in control group (21.18 %, p < 0.05).Conclusion: Xuezhitong capsule, when combined with conventional treatment, exerts high therapeutic effectiveness and safety in MVA patients by inhibiting inflammatory reactions, optimizing endothelialfunction, reducing blood lipid levels, and decreasing the incidence of cardiovascular adverse events. Thus, the combination therapy is a potentially superior therapeutic strategy to the conventional approach for the management of MVA patients.

Therapeutic Effects of Paclitaxel-Loaded Hydroxyapatite-Alginate Beads: In Vitro and In Vivo Studies

2008 ◽  
Vol 396-398 ◽  
pp. 551-554 ◽  
Author(s):  
Tetsuya Abe ◽  
Masataka Sakane ◽  
Toshiyuki Ikoma ◽  
Mihoko Kobayashi ◽  
Naoyuki Ochiai
Keyword(s):  
Cytotoxic Activity ◽  
Survival Time ◽  
Alginate Beads ◽  
Free Time ◽  
Mammary Adenocarcinoma ◽  
Therapeutic Effects ◽  
Composite Beads ◽  
Disease Free

Several drug delivery carriers have reported on local delivery of paclitaxel (PTX), but their effects on intraosseous cancer model are not well known. This study was conducted to clarify the therapeutic effects of our newly developed PTX-loaded HAp-alginate composite beads. Cytotoxic activity was assessed on rat’s mammary adenocarcinoma by cell proliferation assay using WST-1 reagent. Antitumor activity was assessed by 8-week-old rat female Fischer 344 rats of metastatic spine cancer. Twenty-three rats were divided into 3 groups: Group 1 (n = 7) and Group 2 (n = 8) was treated with the PTX-loaded HAp-alginate beads using strontium ions and barium ions, respectively. Group 3 (n = 8) was administered with drug-free HAp-alginate beads. We checked disease-free time and survival time among 3 groups. The HAp-alginate beads containing 2.4wt% of PTX showed significant cytotoxic activity on CRL-1666 cells. The effects were decreased with time during 72 h. The animals treated with 2.4wt% of PTX-loaded HAp-alginate beads showed 40% increase in the disease-free time and 25% increase in survival time. Our studies suggest that newly developed HAp-alginate beads can be a candidate carrier of PTX to bone.

scholarly journals Efficacy of integrating short-course chemotherapy with Chinese herbs to treat multi-drug resistant pulmonary tuberculosis in China: a study protocol

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Shun-Xian Zhang ◽  
Lei Qiu ◽  
Cui Li ◽  
Wei Zhou ◽  
Li-Ming Tian ◽  
...  
Keyword(s):  
Treatment Group ◽  
Pulmonary Tuberculosis ◽  
Therapeutic Effect ◽  
Chinese Herbs ◽  
Control Group ◽  
Drug Resistant ◽  
Chemotherapeutic Drugs ◽  
Cure Rate ◽  
Chemotherapeutic Regimen ◽  
Yin Deficiency

Abstract Background Tuberculosis (TB) caused Mycobacterium tuberculosis (M.tb) is one of infectious disease that lead a large number of morbidity and mortality all over the world. Although no reliable evidence has been found, it is considered that combining chemotherapeutic drugs with Chinese herbs can significantly improves the cure rate and the clinical therapeutic effect. Methods Multi-drug resistant pulmonary tuberculosis (MDR-PTB, n = 258) patients with Qi-yin deficiency syndrome will be randomly assigned into a treatment group (n = 172) or control/placebo group (n = 86). The treatment group will receive the chemotherapeutic drugs combined with Chinese herbs granules (1 + 3 granules), while the control group will receive the chemotherapeutic drugs combined with Chinese herbs placebo (1 + 3 placebo granules). In addition, MDR-PTB (n = 312) patients with Yin deficiency lung heat syndrome will be randomly assigned to a treatment (n = 208) or control/placebo (n = 104) group. The treatment group will receive the chemotherapeutic regimen combined with Chinese herbs granules (2 + 4 granules), while the control group will receive the chemotherapeutic drugs and Chinese herbs placebo (2 + 4 placebo granules). The primary outcome is cure rate, the secondary outcomes included time to sputum culture conversion, lesion absorption rate and cavity closure rate. BACTEC™ MGIT™ automated mycobacterial detection system will be used to evaluate the M.tb infection and drug resistance. Chi-square test and Cox regression will be conducted with SAS 9.4 Statistical software to analyze the data. Discussion The treatment cycle for MDR-PTB using standardized modern medicine could cause lengthy substantial side effects. Chinese herbs have been used for many years to treat MDR-PTB, but are without high-quality evidence. Hence, it is unknown whether Chinese herbs enhances the clinical therapeutic effect of synthetic drugs for treating MDR-PTB. Therefore, this study will be conducted to evaluate the clinical therapeutic effect of combining Chinese herbs and chemotherapeutic drugs to treat MDR-PTB cases. It will assist in screening new therapeutic drugs and establishing treatment plan that aims to improve the clinical therapeutic effect for MDR-PTB patients. Trial registration This trial was registered at ClinicalTrials.gov (ChiCTR1900027720) on 24 November 2019 (prospective registered). Graphical Abstract

First isolated locoregional recurrence following mastectomy for breast cancer: results of a phase III multicenter study comparing systemic treatment with observation after excision and radiation. Swiss Group for Clinical Cancer Research.

1994 ◽  
Vol 12 (10) ◽  
pp. 2071-2077 ◽  
Author(s):  
M Borner ◽  
M Bacchi ◽  
A Goldhirsch ◽  
R Greiner ◽  
F Harder ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Multicenter Study ◽  
Locoregional Recurrence ◽  
Systemic Treatment ◽  
Local Treatment ◽  
Phase Iii ◽  
Survival Duration ◽  
Entire Study ◽  
Study Population ◽  
Disease Free

PURPOSE We performed a randomized phase III multicenter study to compare systemic treatment versus no treatment after complete excision and radiotherapy for isolated first locoregional recurrence in patients with breast cancer. PATIENTS AND METHODS One hundred sixty-seven good-risk patients with an estrogen receptor (ER+) positive recurrence or, in case of unknown receptor status, a disease-free interval (DFI) of greater than 12 months and < or = three recurrent tumor nodules each < or = 3 cm in diameter were entered onto the study. They were randomized to observation subsequent to local treatment or to receive tamoxifen (TAM) until disease progression. Seventy-nine percent of the patients were postmenopausal. RESULTS The median observation period for the entire study population was 6.3 years. The median disease-free survival (DFS) duration was 26 months for observation and 82 months for TAM patients (P = .007). This was mainly due to the reduction of further local recurrences, whereas the occurrence of early distant metastases was delayed. A multivariate analysis identified DFI and treatment with TAM as significant prognostic factors for DFS. The 5-year overall survival (OS) rates were 76% and 74%, respectively (P = .77). DFI was also a prognostic factor for OS. CONCLUSION Systemic therapy with TAM after isolated locoregional recurrence of breast cancer significantly increased 5-year DFS rates from 36% to 59% compared with observation alone and prolonged median DFS by more than 4.5 years in patients with ER+ tumors or in the case of unknown ER status with a DFI of greater than 12 months and minimal tumor burden. Treatment with TAM currently has no significant impact on OS, but the median survival duration of the study population has not yet been reached.

A Controlled Study of LSD Treatment in Alcoholism and Neurosis

1970 ◽  
Vol 116 (533) ◽  
pp. 443-445 ◽  
Author(s):  
R. Denson ◽  
D. Sydiaha
Keyword(s):  
Treatment Group ◽  
Therapeutic Effect ◽  
Standard Treatment ◽  
Psychological Tests ◽  
Controlled Study ◽  
Control Group ◽  
Control Groups ◽  
Waiting Period ◽  
Psychiatric Conditions ◽  
And Control

Favourable results have been reported from the use of lysergide in a wide variety of psychiatric conditions, but controlled studies (2, 3, 4) have not confirmed the existence of a therapeutic effect. In this experiment, alcoholics and neurotics who had been referred by Saskatoon psychiatrists for LSD treatment were allocated at random to Treatment and Control groups after undergoing a series of psychological tests. The members of the Treatment group were offered up to five LSD experiences at intervals of two weeks, whereas those in the Control group were informed that this type of therapy would be made available to them after a twelve-month waiting period. Accounts of the LSD experiences were sent to the referring psychiatrists, who were expected to continue to provide standard treatment to the members of both groups.

Effects of Crude Rhubarb on Intestinal Permeability in Septic Patients

2007 ◽  
Vol 35 (06) ◽  
pp. 929-936 ◽  
Author(s):  
Xue-Ling Fang ◽  
Qiang Fang ◽  
Jian-Jun Luo ◽  
Xia Zheng
Keyword(s):  
Treatment Group ◽  
Therapeutic Effect ◽  
Intestinal Permeability ◽  
Control Group

The therapeutic effect of crude rhubarb on intestinal permeability was investigated in septic patients. Forty septic patients were enrolled in this study and randomly divided into two groups: the crude rhubarb treatment group ( n = 18) and the control group ( n = 22). The same treatments were given to both groups except that the crude rhubarb treatment group was administrated with crude rhubarb powders (3 g, tid, p.o). The levels of procalcitonin, D-lactate in plasma and lactulose/mannitol (L/M) ratio in the urine were determined on the first day and the sixth day after treatment with or without crude rhubarb. There were no significant differences in procalcitonin, L/M ratio and D-lactate on the first day between the crude rhubarb treatment group and the control group ( p > 0.05). However, the ratio of L/M on the sixth day for the control group was 0.167 ± 0.036, while that of the crude rhubarb treatment group was 0.062 ± 0.013 ( p < 0.05). Moreover, the levels of procalcitonin and D-lactate in the crude rhubarb treatment group were obviously lower than those in the control group on the sixth day (procalcitonin: 4.11 ± 1.40 μg/L vs. 2.21 ± 0.61 μg/L ; D-lactate: 0.24 ± 0.06 ng/L vs. 0.09 ± 0.03 ng/L , p < 0.05, both). These data confirmed that crude rhubarb's effects on septic patients of ameliorating intestinal permeability.

scholarly journals Improving the local treatment effect in oral lichen planus

2014 ◽  
Vol 95 (3) ◽  
pp. 338-340
Author(s):  
G I Ron ◽  
G M Akmalova
Keyword(s):  
Treatment Group ◽  
Lichen Planus ◽  
Oral Lichen Planus ◽  
Local Treatment ◽  
Main Group ◽  
Aqua Complex ◽  
Control Group ◽  
Complex Treatment ◽  
Local Use ◽  
Oral Lichen

Aim. To assess the clinical efficacy of local use of titanium glycerosolvate aqua-complex (Tisolum) in combination with hyaluronidase as a component of the complex treatment of oral lichen planus. Methods. The study included 49 patients with typical form of oral lichen planus aged 29 to 65 years. The first group included 25 patients, who received the local treatment with glycerosolvate aqua-complex (Tisolum) in combination with hyaluronidase as a component of the complex treatment. The second group included 24 patients who were treated conventionally. Results. The patients of the treatment group reported the decrease of the oral mucosa tightness and roughness sensation 5.78±0.26 days after the treatment initiation, which was 1.5 times faster compared to the control group (8.6±0.34 days, р 0.05). On the seventh day of treatment, there was a significant papule deflorescence in 19 (76.0%) out of 25 patients of the main group, compared to 11 (45.8%) out of 24 patients of the control group (р 0.05). On the 10-th day, disappearance or downsizing of lesions without signs of active inflammation was observed in all 25 patients of the treatment group, compared to 16 (66.7%) of the control group patients (р 0.05). On the 14-th day, no mucosal lesions were observed in all 25 patients of the treatment group, which was significantly better compared to 9 (37.5%) out of 24 patients of the control group (р 0.05). Regression of clinical signs of the disease at the treatment end was registered in all patients of the main group and in 14 (58.3%) patients of the control group. Conclusion. Local use of composite gel containing titanium glycerosolvate aqua-complex (Tisolum) in combination with hyaluronidase as a component of the complex treatment of oral lichen planus is highly effective.

scholarly journals A Pilot Study Using Nanoparticles and Laser Induced Photothermal Ablation to Treat Low Grade Canine Mast Cell Tumors: Evaluation of Efficacy and Safety

2021 ◽  
Author(s):  
Lisa Parshley ◽  
Lisa A. Miller ◽  
Luis Taboada ◽  
Chelsea Tripp ◽  
Scott Gustafson ◽  
...  
Keyword(s):  
Mast Cell ◽  
Pilot Study ◽  
Treatment Group ◽  
Clinical Remission ◽  
Free Time ◽  
Control Group ◽  
Low Grade ◽  
Tumor Cell Death ◽  
Mast Cell Tumors ◽  
Photothermal Ablation

Abstract Background: Nanoparticles for over forty years have been the subject of a large number of physical and bioscience research. In the last decade use of these particles in medicine has gone from theoretical to clinical trials. Passive targeting of metal-based nanoparticles takes advantage of inherent abnormalities in tumor vasculature allowing accumulation in solid tumors through a process known as the ‘‘enhanced permeability and retention’’ (EPR) effect. In animal tumor implant models, combination of gold and silicone nanoparticle (GSN) and exposure of tumors to laser light (at 808nm) generated enough heat to cause tumor cell death. Mast cell tumors (MCT) are the most common skin tumor in dogs, with an estimate of MCT being roughly 20% of canine skin tumors. The goal of this pilot study is to evaluate nanoparticle and laser tumor thermal ablation on low grade canine MCT model.Results: 38 dogs with 44 mast cell tumors were enrolled in this prospective pilot study. All control tumors and those biopsied prior to laser therapy were found to be low grade MCT by histopathology (two grade method). After random number generation 8 dogs were assigned to control group and 30 dogs were assigned to treatment group. Treatment group had 36 total tumors and control group had 8 tumors present at time of enrollment. Treatment dogs had a 100% response rate, with 94% achieving clinical remission (34 tumors). Recurrence rate was 17% in those tumors achieving clinical remission. Mean progression free time (PFT) for the treatment group was 552 days and mean PFT for the control group was 1095 days.Conclusion: In conclusion results of this study suggest that photothermal ablation using gold-silicone nanoparticles and exposure to near infrared light (808 nm) provides an effective local therapy of low-grade mast cell tumors. Median progression free time and survival was not reached in our treatment group. Suggesting that long term tumor control is possible with PTA that potentially equals surgery when margins are narrow (<0.3cm) or incomplete. PTA appears to have better and more durable MCT responses than either radiation therapy and electrochemotherapy when used as sole therapies.

Sign in / Sign up

Export Citation Format

Share Document