scholarly journals Chemical Compositions and Food Preservation Ability of White Turmeric Rhizomes Essential Oil

2020 ◽  
Vol 3 (2) ◽  
pp. 612-623
Author(s):  
Ngo Thi Thuong ◽  
Truong Minh Luong ◽  
Luu Thi Hue ◽  
Vu Thi Thuy Linh ◽  
Nguyen Thi Bich Thuy ◽  
...  
Keyword(s):  
Essential Oil ◽  
Inhibition Zone ◽  
Food Preservation ◽  
Raw Material ◽  
Chemical Compositions ◽  
Herbal Drug ◽  
Asian Countries ◽  
Inhibition Zone Diameter ◽  
E Coli ◽  
Antibacterial Potential

White turmeric (Curcuma aromatica Salisb.) has been widely used as a traditional herbal drug both in Vietnam and other Asian countries. In this study, the essential oil of white turmeric rhizomes (collected from Dien Bien province) was extracted and evaluated for its chemical composition and antibacterial potential against E. coli and S. aureus. The raw material was 82.48% in moisture and was steam distilled within 3 days from collection at a 0.4 kg L-1 ratio of raw material/equipment volume for 180 minutes. Under these conditions, the essential oil accounted for about 0.3% of the raw material. The GC-MS analysis showed that the composition of the essential oil consisted of more than 46 substances including a-zingiberene, 17.85%; β-sesquiphellandrene, 13.28%; and ar-curcumene, 9.45%. The white turmeric essential oil exhibited antibacterial activity against gram-positive strains of S. aureus with an inhibition zone diameter of 8.0mm but did not inhibit gram-negative strains of E. coli. Importantly, white turmeric essential oil at a concentration of 0.25% could extend the shelf life of mangoes by at least 5 days more than the control samples.

scholarly journals Aktivitas Antibakteri Minyak Atsiri Daun Tembakau terhadap Pertumbuhan Bakteri Escherichia coli dan Staphylococcus aureus

2019 ◽  
pp. 48-56 ◽  
Author(s):  
Elda Nurnasari ◽  
Kristiana Sri Wijayanti
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Inhibition Zone ◽  
Positive Control ◽  
Raw Material ◽  
Diffusion Method ◽  
Tobacco Leaves ◽  
E Coli

Tobacco plants are widely used as raw material for cigarettes, but the results of the study show that in tobacco leaves contain compounds that can be used in the pharmaceutical and health fields. Tobacco leaves contain essential oils that have a distinctive aroma. The study aims to examine the antibacterial activity of tobacco essential oil from six sources tobacco such as Temanggung, Yogyakarta, Purwodadi, Boyolali, Blitar and Probolinggo. Tobacco essential oil is obtained by the steam-water distillation method. Antibacterial activity evaluation was carried out by diffusion method and the minimum inhibitory concentration on two bacteria, Escherichia coli and Staphylococcus aureus. The yield of tobacco essential oil ranged from 0.54-3.39%. Tobacco essential oil had antibacterial activity against bacteria E. coli and S. aureus in the presence of inhibition zones in bacterial media. The biggest inhibition zone was in Yogyakarta tobacco essential oil of 26 mm for S. aureus bacteria, while for E. coli bacteria the biggest inhibition zone was Blitar tobacco essential oil which was equal to 21 mm. The inhibition zone was not much different from the inhibition zone in tetracycline antibiotic testing (positive control) which was 25 mm in S. aureus and 21.5 mm bacteria in E. coli bacteria. Tobacco essential oil from Probolinggo was able to inhibit the growth of S. aureus bacteria up to a concentration of 6.25% and E. coli bacteria to a concentration of 12.5%.

scholarly journals Antibacterial activity of Escherichia coli from squid ink (Loligo sp.) n-Hexane extracts

2021 ◽  
Vol 869 (1) ◽  
pp. 012033
Author(s):  
D R Utami ◽  
I Irwan ◽  
S Agustina ◽  
S Karina ◽  
S Afriani
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Secondary Metabolite ◽  
Bioactive Compound ◽  
Inhibition Zone ◽  
Hexane Extract ◽  
Inhibition Zone Diameter ◽  
E Coli ◽  
Hexane Extracts ◽  
Intermediate Concentration

Abstract Squid is one of the export commodities in Indonesia. In general, the use of squid meat, while the ink is only as waste. In fact, Squid ink contain bioactive compound that potential as anti-inflammatory, antihypertensive, anti-diabetic,anti-microbial and anti-malaria agents. The purpose of the study is to determine the types of secondary metabolite compounds contained in n-hexane extract of Loligo sp. ink using maceration method to determine its antibacterial activity against Escherichia coli. The results of secondary metabolite compounds obtained from the n-hexane extract of Loligo sp. ink are alkaloid, saponins, glycosides and phenol. The results of antibacterial test against E. coli using the disc method obtained the average of inhibition zone diameter at the concentration of 4% is 6.3 mm (intermediate), concentration of 8% is 7.83 mm (intermediate), concentration of 16% is 14.5 mm (susceptible) and concentration of 32% is 10.83 mm (intermediate). The antibacterial activity in n-hexane extract of Loligo sp. ink is optimal at the concentration of 16% against E. coli bacteria.

scholarly journals ANTIBACTERIAL ACTIVITY OF CHITOSAN AND HYDROLYZED COCONUT OIL AND THEIR COMBINATION AGAINST BACILLUS CEREUS AND ESCHERICHIA COLI

2016 ◽  
Vol 9 (5) ◽  
pp. 69 ◽  
Author(s):  
Jansen Silalahi ◽  
Petrika Situmorang ◽  
Popi Patilaya ◽  
Yosy Ce Silalahi
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Bacillus Cereus ◽  
Coconut Oil ◽  
Inhibition Zone ◽  
Research Centre ◽  
Virgin Coconut Oil ◽  
Inhibition Zone Diameter ◽  
E Coli ◽  
Zone Diameter

ABSTRACTObjective: The purpose of this study was to evaluate the antibacterial activity of chitosan, hydrolyzed coconut oil and their combination againstBacillus cereus and Escherichia coli.Methods: The materials used in this study were powder of chitosan (obtained from prawn shell produced by Laboratory of Research Centre FMIPAUniversity of Sumatera Utara) and virgin coconut oil (VCO) product of Siti Nurbaya-Indonesia. VCO was partially hydrolyzed by Lipozyme TL IM(active at sn-1,3 position) and the result called hydrolyzed virgin coconut oil (HVCO). The bacteria used in this study were B. cereus and E. coli. Theantibacterial activity of chitosan in 1% acetic acid and HVCO in dimethylsulfoxide was tested by Kirby–Bauer agar diffusion method using paper discwith diameter of 6 mm.Results: The results showed that the minimum inhibitory concentration of chitosan against B. cereus and E. coli is at concentration of 0.05% withinhibition zone diameter of 6.86 mm and 7.56 mm, respectively. MIC of HVCO against B. cereus is at concentration of 0.25% with inhibition zonediameter of 6.40 mm, and against E. coli is at a concentration of 0.50% with inhibition zone diameter of 6.20 mm. The inhibition zone diameter ofchitosan 0.05% and HVCO 0.25% in combination against B. cereus is 8.33 mm which is higher than half the sum of chitosan 0.05% and HVCO 0.25%(6.63 mm). The inhibition zone diameter of chitosan 0.05% and 0.5% HVCO in combination against E. coli is 8.53 mm which is higher than half thesum of chitosan 0.05% and HVCO 0.5% (6.53 mm).Conclusion: The findings of this study indicate that chitosan is more antibacterial than HVCO, and the interaction between chitosan and HVCO incombination demonstrated to be synergistic against B. cereus and E. coli.Keywords: Antibacterial, Chitosan, Coconut oil, Combination, Bacillus cereus, Escherichia coli.®

scholarly journals UJI AKTIVITAS ANTIBAKTERI DAUN JERUK KALAMANSI (Citrofortunella microcarpa) TERHADAP BAKTERI Staphylococcus aureus DAN Escherichia coli

Alotrop ◽  
2019 ◽  
Vol 3 (2) ◽  
Author(s):  
Deza Oktasila ◽  
Nurhamidah Nurhamidah ◽  
Dewi Handayani
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Raw Material ◽  
Diffusion Method ◽  
Citrus Leaves ◽  
Escherichia Coli Bacteria

This study aims to examine the antibacterial activity of ethanol extract and essential oil of Kalamansi citrus leaves (Citrofortunella microcarpa) against Staphylococcus aureus and Escherichia coli bacteria. The raw material of Kalamansi citrus leaves is obtained from the Village of Pondok Kubang, Bengkulu Tengah (3.70060S, 102.35780E). Ethanol extract from Kalamansi citrus leaves was obtained by maceration using ethanol 96%, then ethanol extract was made dilution concentration 40 ; 20; , 10; and 5%. The essential oil of Kalamansi citrus leaves is obtained by water-vapor distillation, then made variations of concentration 20;, 15;, 10;  and 5%. The method used to test the antibacterial activity is the paper disc diffusion method , the antibacterial activity is shown by the diameter of the inhibiting  zone formed. The data of antibacterial test result were analyzed by using One Way Anova test which showed the effect of treatment on the growth of test bacteria seen from the value (P <0,01) and continued by Duncan test to know the effect of the treatment. The results showed that ethanol extract of Kalamansi citrus leaves had antibacterial activity against S. aureus and E. coli bacteria with moderate inhibitory diameter is  7.20 and 5.73 mm at concentration 40%, while antibacterial activity on essential oil of Kalamansi citrus leaves is categorized as strong with inhibition zone diameter is 14.83 and 13.00 mm at concentration 20%.

scholarly journals Antibacterial Activity and Interaction Effects of Litsea Cubeba Fruit Essential Oil and Chitosan Against Food-Borne Bacteria  

2021 ◽  
Vol 31 (3) ◽  
pp. 1-6
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Inhibitory Concentration ◽  
Synergistic Effects ◽  
Inhibition Zone ◽  
Food Preservation ◽  
Individual Treatment ◽  
Fractional Inhibitory Concentration ◽  
Litsea Cubeba ◽  
Food Borne

The aims of this study were to investigate the antibacterial activity and interaction effect of Litsea cubeba fruit essential oil (EO) and chitosan (CTS) against food-borne bacteria. The inhibition zone of EO, CTS and EO-CTS mixture against Escherichia coli ATCC 25922, Salmonella typhimurium ATCC 14028, Staphylococcus aureus ATCC 25923 and Bacillus cereus ATCC 13061 ranged from 39.0 - 57.3 mm; 11.7 - 15.5 mm and 29.7 - 39.3 mm, respectively. The minimum inhibitory concentration (MIC) of EO and CTS were 5.53 mg/mL and from 0.60 - 0.80 mg/mL, respectively. The synergistic effects were found when EO and CTS were used in combination with fractional inhibitory concentration values ranging from 0.62 - 0.67. The MIC of the EO and CTS combination was decreased from 6 to 8-fold and 2-fold compared to individual treatment by EO and CTS, respectively. The obtained results demonstrated the potential application of EO-CTS mixture in food preservation.

The chemical composition, antimicrobial, and antioxidant activities of Pycnocycla spinosa and Pycnocyla flabellifolia essential oils

2016 ◽  
Vol 71 (11-12) ◽  
pp. 403-408 ◽  
Author(s):  
Mohaddese Mahboubi ◽  
Elaheh Mahdizadeh ◽  
Rezvan Heidary Tabar
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Antioxidant Activities ◽  
Inhibition Zone ◽  
Chemical Compositions ◽  
Caryophyllene Oxide ◽  
Aerial Parts ◽  
Dilution Assay ◽  
Main Components ◽  
Broth Dilution

Abstract The purpose of our study was to compare the chemical compositions and antimicrobial and antioxidant activities of Pycnocycla spinosa and Pycnocycla flabellifolia essential oils. cis-Asarone (62.5%) and widdra-2,4(14)-diene (9%) were the main components of P. spinosa aerial part essential oil, while elemicin (60.1%) and caryophyllene oxide (9.8%) were the main components of P. spinosa seed essential oil. α-Phellandrene (25.5%), p-cymene (15.3%), and limonene (13.3%) were found in P. flabellifolia essential oil. The inhibition zone diameters for P. flabellifolia essential oil were significantly higher than for the two other essential oils from P. spinosa (p<0.05). In broth dilution assay (µL/mL), the sensitive microorganism to Pycnocycla sp. (P. spinosa, P. flabellifolia) was Aspergillus niger, followed by Candida albicans. In 2,2-diphenyl-1-picrylhydrazyl (DPPH) system, P. spinosa aerial parts essential oil (IC50=548 µg/mL) had higher antioxidant activity than that of two other essential oils.

scholarly journals Antimicrobial Study of Chloroform Fraction from the Leaves of Entada spiralis Ridl.

2021 ◽  
Vol 1 (1) ◽  
pp. 45-53
Author(s):  
Fitri Rachmadita ◽  
Erryana Martati ◽  
Sharifah Nurul Akilah Syed Mohamad ◽  
Siti Zaiton Mat So'ad
Keyword(s):  
Antimicrobial Activity ◽  
Bioactive Compounds ◽  
Crude Extract ◽  
Inhibition Zone ◽  
Food Preservation ◽  
Total Phenolic ◽  
Chloroform Fraction ◽  
Disk Diffusion Test ◽  
E Coli

Introduction: Entada Spiralis Ridl., or locally identified as Sintok, contains flavonoid, saponin, tannin, and glycoside, compounds that have antifungal and antibacterial activities. This research aims to identify bioactive compounds and determine the antimicrobial activity from crude and fraction of E. spiralis extract. Methods: The crude extract was prepared by macerating the leaves with chloroform, and then proceeded to fraction it by vacuum liquid chromatography with Dichloromethane (DCM)/Hexane (Hex) (1/9) and Dichloromethane (DCM)/Methanol (MeOH) (9/1) solvent system. Disk Diffusion Test and Microdilution Assay evaluated the extracts' antimicrobial activity against S. aureus, E. coli and C. albicans. The determination of bioactive compounds was done by Thin Layer Chromatography (TLC). Determination of Total Phenolic (TPC) and Flavonoid Content (TFC) were performed by Folin-Ciocalteu and AlCl3 Colourimetric Assay Results: The greatest inhibition zone against C. albicans was obtained from fraction Chloroform (CHCl3) extract with an inhibition zone of 10.33 mm. DCM/MeOH (9/1) effectively killed S. aureus and E.coli with an inhibition zone of 11.67 and 12 mm, respectively. The minimum inhibitory concentration (MIC) of CHCl3 crude extract were 1.563 mg/mL for both E. coli and S. aureus, and 0.781 mg/mL for C. albicans. The TLC revealed the presence of tannins, saponin, glycosides, phenol, flavonoid, triterpenoid, and aromatic compound in CHCl3 crude extract. TPC of DCM/MeOH (9/1), CHCl3, and DCM/Hex (1/9) were 50.56 ± 0.188, 51.913± 0.089, 24.16 ± 0.175 mg GAE/g extract. Conclusion: In conclusion, E. spiralis leaves could be a source of active antifungal and antimicrobial agents used for food preservation by using a semipolar solvent for extraction.

scholarly journals Chemical composition and biological activity of Peucedanum dhana A. Ham essential oil

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Sarunpron Khruengsai ◽  
Teerapong Sripahco ◽  
Narawadee Rujanapun ◽  
Rawiwan Charoensup ◽  
Patcharee Pripdeevech
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Membrane Integrity ◽  
Enterobacter Aerogenes ◽  
Inhibition Zone ◽  
Gas Chromatography Mass Spectrometry ◽  
E Coli ◽  
Murine Fibroblast ◽  
Pure Compounds ◽  
Lung Adenocarcinoma A549

AbstractThe essential oil was extracted from Peucedanum dhana A. Ham, which grows in Thailand, using a Clevenger apparatus, resulting in an oil yield of 0.76% w/w. Forty-two compounds were identified using gas chromatography-mass spectrometry. The major compounds were trans-piperitol (51.23%), β-pinene (11.72%), o-cymene (11.12%), γ-terpinene (9.21%), and limonene (4.91%). The antimicrobial activity of the P. dhana essential oil was investigated by measuring the inhibition zone diameter, minimum inhibitory concentration (MIC), and minimum microbicidal concentration (MMC). The inhibition zone diameters of P. dhana essential oil (1000 µg/mL) against tested pathogens ranged from 10.70 to 40.80 mm. Significant antimicrobial activity against tested pathogens was obtained, with MIC and MMC values of 62.50–250 µg/mL and 250–1000 µg/mL, respectively. Escherichia coli, Pseudomonas aeruginosa, and Enterobacter aerogenes exposed to P. dhana essential oil at the MIC were analysed by flow cytometry using propidium iodide (PI) and SYTO9 to assess membrane integrity compared to trans-piperitol and β-pinene. After 24 h, treatments with trans-piperitol resulted in the most significant cell membrane alteration and depolarization followed by P. dhana essential oil and β-pinene, respectively. It was demonstrated that the P. dhana essential oil presented antibacterial action against E. coli, P. aeruginosa, and E. aerogenes. The antioxidant activity of P. dhana essential oil was measured using 2,2-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium (ABTS) scavenging activity assays. The IC50 values obtained from the DPPH and ABTS methods were 9.13 and 9.36 mg/mL, respectively. The cytotoxic effect of P. dhana oil was tested against human colonic adenocarcinoma (SW480), human lung adenocarcinoma (A549), cervical cancer (Hela), and murine fibroblast (3T3L1) cells using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The essential oil had cytotoxicity against all cancer cells, with significant cytotoxicity towards SW480 cells. As a control experiment, two pure compounds—trans-piperitol and β-pinene, were also tested for their antimicrobial, antioxidant, and cytotoxic activity. Both compounds showed varied activity in all assays. The results indicate that P. dhana essential oil could be used as a source of functional ingredients in food and pharmaceutical applications.

scholarly journals SKRINING FITOKIMIA DAN UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN RACUN AYAM (Brucea javanica [L.] Merr.) ASAL KALIMANTAN SELATAN

2021 ◽  
Vol 6 (1) ◽  
pp. 59-68
Author(s):  
Sutomo Sutomo ◽  
◽  
Arnida Arnida ◽  
Fahriah Fahriah
Keyword(s):  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Negative Control ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Inhibition Zone Diameter ◽  
E Coli ◽  
Zone Diameter ◽  
Brucea Javanica

Racun ayam is one of the plants which has several activities in its fruits, one of which is an antibacterial. This study aims to determine the antibacterial activity of ethanol extract of racun ayam leaves. The extraction was done by maceration method using ethanol as a solvent. Antibacterial activity assay was carried out on S. aureus, E. coli, and P. aeruginosa bacteria by disk diffusion method at the concentrations of 80, 100, and 120 ppm. The results showed that the ethanol extract of B. javanica leaves had the greatest inhibition zone on E. coli with inhibition zone diameter values ​​at 80, 100, and 120 ppm were 11.7 mm, 14.5 mm and 19.67 mm respectively. Inhibition zones in S. aureus could only be seen at 100 and 120 ppm which had inhibition zone diameters at 1.17 mm and 2.17 mm respectively, whereas in P. aeruginosa they did not have any inhibitory zone values. The results of statistical analysis showed a significance of the inhibition zone diameter values ​​of E. coli bacteria starting at 80 ppm with a sig value of 0.034. Due to a sig value was <0.05, it was categorized as significantly different from the negative control. The bacteria of S. aureus which had ​​<0.05 for sig values only found on 100 ppm and 120 ppm. Bacteria P. aeruginosa which had sig value >0.05 was not significantly different at all concentrations

scholarly journals Antibacterial Activities of Perfume: Combination Flower Magnolia alba, Cananga odorata and Mimusops elengi L, Fixed with Pogostemon cablin Oil

2019 ◽  
Vol 2 (1) ◽  
pp. 19-23
Author(s):  
Rosnani Nasution ◽  
Azalia Izdhihar Azwar ◽  
Hira Helwati ◽  
Marianne
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Inhibitory Zone ◽  
E Coli ◽  
Pogostemon Cablin ◽  
Inhibitory Power ◽  
Mimusops Elengi ◽  
Cananga Odorata

Abstract: The making of perfume from the combination Magnolia  alba (M. alba) flower oil, Cananga odorata (C. odorata) and Mimusops elengi L (M. elengi) fixed with Pogostemon cablin oil (P. cablin) have been done. Essential oil of M. alba and C. odorata was distilled by water distillation and extraction essential oil of M. elengi flower using n-hexane evaporated solvent method. Composition perfume through organoleptic test obtained with a ratio of 4% v/v, 4% v/v, and 2% v/v for each essential oil M. alba, C. odorata and M. elengi. Antibacterial activity of the perfume and each of the volatile oil was performed by the disc diffusion method. Antibacterial activity test showed that perfumes were able to inhibit the growth of Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) bacteria, but less active. Antibacterial activity of the perfume was more active against E. coli with an inhibitory zone of 8.3 mm and 39.81% inhibitory power  than S. aureus bacterial 7.3 mm inhibition zone with 37.55% inhibitory power. Perfumes after storage for  one, and two months had better antibacterial activity than before storage with an average inhibitory zone of 11-15 mm and inhibitory power of 50- 68,18%.   Keywords: Antibacterial, E. Coli, C. Odorata, M. alba, M. elengi, P. cablin, , S. aureus  

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