Antioxidant and Sunscreen Activities from Blackberry Extract in vitro using Spectrophotometry UV-Visible

In recent years, green nanotechnology-based approaches using plant materials have been accepted as an environmentally friendly and cost-effective approach with various biomedical applications. In the current study, AgNPs were synthesized using the seed extract of the Eugenia uniflora L. (E.uniflora). Characterization was done using UV-Visible spectroscopy, X-ray diffraction (XRD), scanning electronic microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX) analyses. The formation of AgNPs has confirmed through UV-Visible spectroscopy (at 466 nm) by the change of color owing to surface Plasmon resonance. Based on the XRD pattern, the crystalline property of AgNPs was established. The functional group existing in seed of E.uniflora extract accountable for the reduction of Ag+ ion and the stabilization of AgNPs was investigated. The morphological structures and elemental composition was determined by SEM and EDX analysis. With the growing application of AgNPs in biomedical perspectives, the biosynthesized AgNPs were evaluated for their antibacterial and along with their antidiabetic potential. The results showed that AgNPs are extremely effective with potent antidiabetic potential at a very low concentration. It also exhibited potential antibacterial activity against the three tested human pathogenic bacteria. Overall, the results highlight the effectiveness and potential applications of AgNPs in biomedical fields such as in the treatment of acute illnesses as well as in drug formulation for treating various diseases such as cancer and diabetes. It could be concluded that E. uniflora seed extract AgNPs can be used efficiently for in vitro evaluation of their antibacterial and antidiabetic effects with potent biomedical applications.

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Synthesis, Characterization, Antimicrobial and Antioxidant Potential of Diazenyl Chalcones

Current Topics in Medicinal Chemistry ◽

10.2174/1568026618666180626095714 ◽

2018 ◽

Vol 18 (10) ◽

pp. 844-856 ◽

Cited By ~ 5

Author(s):

Harmeet Kaur ◽

Balasubramanian Narasimhan

Keyword(s):

Radical Scavenging Activity ◽

Radical Scavenging ◽

Antioxidant Potential ◽

Dilution Method ◽

Dpph Assay ◽

Antimicrobial Potential ◽

Structural Conformation ◽

Uv Visible ◽

Tube Dilution Method

A series of diazenyl chalcones was prepared by base catalyzed Claisen-Schmidt condensation of synthesized hydroxy substituted acetophenone azo dye with various substituted aromatic/ heteroaromatic aldehydes. The structural conformation of synthesized chalcones was done by a number of physicochemical and spectral means like FTIR, UV-visible, mass, NMR spectroscopy and CHNS/O analysis. These diazenyl chalcones were assessed for their in vitro antimicrobial potential against several Gram-negative, Gram-positive bacterial and fungal strains by serial tube dilution method. The fluconazole and cefadroxil were used as standard drugs. The target compounds were also evaluated for their antioxidant potential by DPPH assay. (2E)-3-(2,4-Dichlorophenyl)-1-(4-((2,6- dihydroxyphenyl)diazenyl)phenyl)prop-2-en-1-one (C-7) had shown very good antimicrobial potential with MIC ranges from 3.79 to 15.76 μg/ml against most of the tested microorganisms. Most of the synthesized diazenyl chalcones were found to be active against B. subtilis. The (2E)-1-(5-((2-Chloro- 4-nitrophenyl)diazenyl)-2-hydroxyphenyl)-3-(2-hydroxynaphthalen-1-yl)prop-2-en-1-one (C-10) had shown high free radical-scavenging activity when compared with the ascorbic acid as the reference antioxidant.

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Shaping and Cellular Uptake of Folic Acid Coated Gold and Magnetite Nanoparticles

Current Nanomedicine ◽

10.2174/2468187308666180913113827 ◽

2019 ◽

Vol 9 (2) ◽

pp. 166-172

Author(s):

Ahmed A.G. El-Shahawy ◽

Gamal Elghnam ◽

Alsayed A.M. Alsherbini

Keyword(s):

Folic Acid ◽

Iron Oxide ◽

Tumor Cells ◽

Iron Oxide Nanoparticles ◽

Individual Cell ◽

Oxide Nanoparticles ◽

Cellular Compartment ◽

Specific Tumor ◽

Uv Visible

Background:Gold and Iron Oxide nanoparticles NPs play as nanocarriers for a specific drug delivery and contrast agents. Intercellular uptake of these nanoparticles and targeting to individual cell and sub-cellular compartment is essential.Objective:The aim of the current study is to evaluate the intracellular uptake of these NPs to specific tumor cells in vitro conjugated with folic acid with a goal of enhancing the efficiency of specific targeting to tumor cells.Methods:We synthesized the nanoparticles by a chemical method and characterized by UV-Visible, FTIR, XRD, and TEM.Results & Conclusion:The results revealed the conjugation of Gold and Iron Oxide nanoparticles with folic acid increased the intercellular uptake with high percent compared to non- conjugated nanoparticles.

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Synthesis, characterization, and pharmacological evaluation of thiourea derivatives

Open Chemistry ◽

10.1515/chem-2020-0139 ◽

2020 ◽

Vol 18 (1) ◽

pp. 764-777

Author(s):

Sumaira Naz ◽

Muhammad Zahoor ◽

Muhammad Naveed Umar ◽

Saad Alghamdi ◽

Muhammad Umar Khayam Sahibzada ◽

...

Keyword(s):

Spectroscopic Techniques ◽

Organosulfur Compounds ◽

Bacterial Strains ◽

Thiourea Derivatives ◽

Toxicological Effects ◽

Pharmaceutical Industries ◽

Uv Visible ◽

Hematological And Biochemical Parameters

AbstractThioureas and their derivatives are organosulfur compounds having applications in numerous fields such as organic synthesis and pharmaceutical industries. Symmetric thiourea derivatives were synthesized by the reaction of various anilines with CS2. The synthesized compounds were characterized using the UV-visible and nuclear magnetic resonance (NMR) spectroscopic techniques. The compounds were screened for in vitro inhibition of α-amylase, α-glucosidase, acetylcholinesterase (AChE), and butyrylcholinesterase (BuChE) enzymes and for their antibacterial and antioxidant potentials. These compounds were fed to Swiss male albino mice to evaluate their toxicological effects and potential to inhibit glucose-6-phosphatase (G6Pase) inhibition. The antibacterial studies revealed that compound 4 was more active against the selected bacterial strains. Compound 1 was more active against 2,2-diphenyl-1-picrylhydrazyl and 2,2’-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) free radicals, AChE, BuChE, and α-glucosidase. Compound 2 was more potent against α-amylase and G6Pase. Toxicity studies showed that compound 4 is safe as it exerted no toxic effect on any of the hematological and biochemical parameters or on liver histology of the experimental animals at any studied dose rate. The synthesized compounds showed promising antibacterial and antioxidant potential and were very active (both in vitro and in vivo) against G6Pase and moderately active against the other selected enzymes used in this study.

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The Study of Albumin Release from Silica/Albumin as a Potential Drug Delivery Carrier

Jurnal Teknologi ◽

10.11113/jt.v69.3216 ◽

2014 ◽

Vol 69 (5) ◽

Author(s):

Shafiyah Pondi ◽

Jon Efendi ◽

Ho Chin Siong ◽

Lai Sin Yuan ◽

Sheela Chandren ◽

...

Keyword(s):

Drug Delivery ◽

Fumed Silica ◽

In Vitro Release ◽

Phosphate Buffer Solution ◽

Buffer Solution ◽

Solution Ph ◽

Drug Delivery Carrier ◽

High Interaction ◽

Uv Visible

The drug-delivery field has been an attractive as well as challenging area for research. With the emerging of new formulated drugs and pharmaceutical compounds, development of good drug-delivery system (DDS) is crucially required. This study aims to utilize albumin as the drug template in silica/albumin/drug (S/A/D) system. Prior to designing this system, the interaction between silica and albumin was investigated. It is hypothesized that high interaction between silica and albumin may result in slower drug release over time, which is preferred for a good DDS. Silica and albumin (S/A) materials were prepared by using fumed silica and tetraethyl orthosilicate (TEOS) as the silica precursors. Three different S/A samples were prepared; fumed silica with albumin (FS/A), fumed silica with pre-treated albumin by sodium borohydrate (FS/A-N), and silica sol (TEOS) with albumin (SS/A). In-vitro release of albumin in phosphate buffer solution (pH 7) was carried out to examine the interaction between albumin and silica. The concentration of albumin was detected at 280 nm by UV-visible spectrophotometer. All samples were characterized by diffuse reflectance-UV-visible spectrophotometer (DR-UV), Fourier transform infrared spectrophotometer (FTIR) dan thermogravimetric-differential thermal analysis (TG-DTA). DR-UV results show that SS/A exhibited the lowest absorption intensity at 280 nm, which indicates better interaction between silica and albumin. This result was supported by the presence of Si-O stretching band of silanol at 952 cm-1 from the FTIR spectrum. Release study of albumin demonstrated that the release of albumin from SS/A was slowest compared to those of FS/A and FS/A-N.

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Synthesis and Bioassay of Novel Quinozolins

journal of the college of basic education ◽

10.35950/cbej.v17i72.4505 ◽

2019 ◽

Vol 17 (72) ◽

pp. 129-138

Author(s):

Yasmine Kadom. Al-Majedy

Keyword(s):

Twist Angle ◽

Antimicrobial Properties ◽

Heat Of Formation ◽

Gram Negative Bacteria ◽

The Novel ◽

Gram Positive Bacteria ◽

Visible Spectra ◽

Ft Ir ◽

Uv Visible

Novel Quinozolins were synthesized in a good yield through convert lacton to lactam and study the biological activity of the synthesized compounds. Quinozolins were characterized by elemental analysis, FT-IR and UV/visible spectra. The novel Quinozolins have been tested in vitro against (gram positive bacteria Staphylococcus aureus and against other gram negative bacteria, such as Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Proteus vulgaris; in order to assess their antimicrobial properties. Moreover, charge, bond length, bond angle, twist angle, heat of formation and steric energy were calculated by using of the ChemOffice program. The study indicates that these Quinozolins have high activity against tested bacteria. Based on the reported results, it may be concluded that the coumarin act as synthons for synthesis of new Quinozolins derivatives through the replacement of oxygen atom by nitrogen atom.

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Structural Basis of Binding and Rationale for the Potent Urease Inhibitory Activity of Biscoumarins

BioMed Research International ◽

10.1155/2014/935039 ◽

2014 ◽

Vol 2014 ◽

pp. 1-12 ◽

Cited By ~ 6

Author(s):

Muhammad Arif Lodhi ◽

Sulaiman Shams ◽

Muhammad Iqbal Choudhary ◽

Atif Lodhi ◽

Zaheer Ul-Haq ◽

...

Keyword(s):

Molecular Docking ◽

Active Sites ◽

Docking Simulation ◽

Cysteine Residue ◽

Structural Basis ◽

Bacillus Pasteurii ◽

Nickel Ions ◽

Jack Bean ◽

Uv Visible

Urease belongs to a family of highly conserved urea-hydrolyzing enzymes. A common feature of these enzymes is the presence of two Lewis acid nickel ions and reactive cysteine residue in the active sites. In the current study we examined a series of biscoumarins1–10for their mechanisms of inhibition with the nickel containing active sites of Jack bean andBacillus pasteuriiureases. All these compounds competitively inhibited Jack bean urease through interaction with the nickel metallocentre, as deduced from Michaelis-Menten kinetics, UV-visible absorbance spectroscopic, and molecular docking simulation studies. Some of the compounds behaved differently in case ofBacillus pasteuriiurease. We conducted the enzyme kinetics, UV-visible spectroscopy, and molecular docking results in terms of the known protein structure of the enzyme. We also evaluated possible molecular interpretations for the site of biscoumarins binding and found that phenyl ring is the major active pharmacophore. The excellent in vitro potency and selectivity profile of the several compounds described combined with their nontoxicity against the human cells and plants suggest that these compounds may represent a viable lead series for the treatment of urease associated problems.

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FTIR and UV‒vis study of chemically engineered biomaterial surfaces for protein immobilization

Spectroscopy An International Journal ◽

10.1155/2002/183053 ◽

2002 ◽

Vol 16 (3-4) ◽

pp. 351-360 ◽

Cited By ~ 51

Author(s):

Herman Mansur ◽

Rodrigo Oréfice ◽

Marivalda Pereira ◽

Zélia Lobato ◽

Wander Vasconcelos ◽

...

Keyword(s):

Colorimetric Assay ◽

Protein Immobilization ◽

Research Field ◽

Male Rats ◽

Visible Spectroscopy ◽

Functional Abnormality ◽

Specific Chemical ◽

Uv Visible

The biomaterials research field has broadened in the last 3 decades, including replacement of diseased or damaged parts, assist in healing, correct and improve functional abnormality, drug delivery systems, immunological kits and biosensors. Proteins play crucial role in almost every biological system. They are involved in enzymatic catalysis, transport and storage, coordinated motion, mechanical support, immune protection, control of growth and cell differentiation among many others. The immobilization of proteins onto surface functionalized substrates has been one of the most promising areas in bioengineering field. It is important to note that the term immobilization can refer either to a temporary or to a permanent localization of the biomolecule on or within a support. Proteins have very particular chain configurations and conformations that promote high levels of specificity during chemical interactions. In the present work, we aimed to study the phenomenon of protein immobilization onto biomaterial with chemically engineered surface. We have tailored the surface of the porous gels of SiO2with 5 different silane surface modifying agents: tetraethoxysilane (TEOS), 3‒mercaptopropyltrimethoxysilane (MPTMS) and 3‒aminopropyltriethoxysilane (APTES), 3‒glycidoxypropyltrimethoxysilane (GPTMS) and 3‒isocyanatopropyltriethoxysilane (ICPES). Fourier Transform Infrared Spectroscopy (FTIR) was used to characterize the presence of all specific chemical groups in the materials. The surface functionalized gels were then immersed in porcine insulin (PI) solutions for protein immobilization. The incorporation of protein within the gels was also monitored by FTIR spectroscopy. The kinetics of protein adsorption and desorption from the gel matrixin vitrotests were monitored by UV‒visible spectroscopy. We could not observe any evidence of denaturation of insulin after its desorption from gel matrices using UV‒visible spectroscopy technique.In vivotests with adult male rats were used to verify the immobilized insulin bioactivity after implantation of different biomaterial with functionalized surfaces. Plasma glucose levels were obtained by using the Glucose GOD‒ANA Colorimetric Assay. All surface modified materials have presented acute hypoglycemic peak response associated with the insulin bioactivity.

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Delivery of Ursolic Acid by Polyhydroxybutyrate Nanoparticles for Cancer Therapy: in silico and in vitro Studies

Drug Research ◽

10.1055/a-1640-0009 ◽

2021 ◽

Author(s):

Sureshbabu Ram Kumar Pandian ◽

Selvaraj Kunjiappan ◽

Parasuraman Pavadai ◽

Velmurugan Sundarapandian ◽

Vivek Chandramohan ◽

...

Keyword(s):

Ursolic Acid ◽

Growth Factor Receptor ◽

Maximum Efficiency ◽

Release Behavior ◽

Drug Release Behavior ◽

Pentacyclic Triterpenoid ◽

Epidermal Growth ◽

Uv Visible ◽

Average Size

AbstractUrsolic acid (UA), a pentacyclic triterpenoid and a phytochemical, is a potent inhibitory agent against proliferation of various tumors. Polyhydroxybutyrate nanoparticles (PHB NPs) are preferred in therapeutics due to their drug-stabilizing property and enhanced biological activity. In this study, PHB NPs were utilized to deliver and enhance the bioavailability of UA against cancer cells (HeLa). Further, molecular docking and dynamic studies were conducted to calculate the binding affinity and stability of UA at the active site of target protein (epidermal growth factor receptor-EGFR). The PHB NPs revealed the average size as 150–200 nm in TEM, which were used in subsequent experiments. The cytoplasmic uptake of nanoparticles was confirmed by florescent microscopy. The encapsulation potential of PHB NPs with UA was assessed by UV–visible spectrophotometer as 54%. Besides, the drug release behavior, cytotoxicity and the regulation of apoptosis were investigated in vitro. The cytotoxicity results revealed that the maximum efficiency of drug delivery was at 96th hour.

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Synthesis and Antimicrobial Resistant Modulatory Activity of 2,4-Dinitrophenylhydrazone Derivatives as Agents against Some ESKAPE Human Pathogens

Journal of Chemistry ◽

10.1155/2020/2720697 ◽

2020 ◽

Vol 2020 ◽

pp. 1-9

Author(s):

Alberta Ade ◽

Cedric D. K. Amengor ◽

Abena Brobbey ◽

Isaac Ayensu ◽

Benjamin K. Harley ◽

...

Keyword(s):

Streptococcus Pneumoniae ◽

Klebsiella Pneumoniae ◽

Inhibitory Concentration ◽

Fungal Species ◽

Human Pathogens ◽

Visible Spectroscopy ◽

Active Compounds ◽

Gram Negative ◽

Uv Visible

A library of six novel phenylhydrazones were synthesized and evaluated for their in vitro antimicrobial and resistance modulating activity against a panel of Gram-positive, Gram-negative, and fungal species. The compounds were produced in good yields of 60–92% w/w and characterized using melting point, UV-visible spectroscopy, infrared, and nuclear magnetic resonance (1H, 13C, and DEPT-Q) techniques. Mass spectroscopy was used to confirm the identity of one of the most active compounds, 5 [SA5]. The phenylhydrazones showed activity against all the six selected microorganisms with minimum inhibitory concentration (MIC) values of the most active compounds, 1 [BP1] and 5 [SA5], at 138 µM (Klebsiella pneumoniae) and 165 µM (Streptococcus pneumoniae), respectively. Compound 1 [BP1] further demonstrated a high resistance modulatory activity at 1.078 µM against Streptococcus pneumoniae and Klebsiella pneumoniae.

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