Green Biosynthesis of Iron Oxide Nanoparticles and Testing Their Inhibitory Efficacy Against Some Pathogens

2021 ◽  
Vol 18 (4) ◽  
pp. 119-123
Author(s):  
Doaa Kaduim ◽  
Zaid Mahmoud ◽  
Falah Mousa
Keyword(s):  
Iron Oxide ◽  
Beta Vulgaris ◽  
Inhibition Zone ◽  
Nano Particles ◽  
X Ray Diffraction ◽  
X Ray ◽  
E Coli ◽  
Size Of Particles ◽  
Xrd Patterns ◽  
Antibacterial And Antifungal

The biosynthesis of iron oxide (Fe2O3, also known as haematite) nano particles (NPs) using Hydra helix and Beta vulgaris aqueous extracts were adduced, respectively, where the extracts act as a stabiliser and reductant reagent. The crystal structure and size of particles were investigated using X-ray diffraction (XRD), while the morphology was examined using field emission scanning electron microscopy (FESEM), XRD patterns showed the synthesised nanoparticles with well-crystallised structure from Beta vulgaris extract with size 12 nm, while the results by using Hydra helix showed many peaks back to Goethite phase with 16 nm. The antibacterial and antifungal activity were examined using Staphylococcus (showed inhibition zone diameter 23 mm, 16 mm using Hydra helix and Beta vulgaris, respectively), E. coli (showed no inhibition) and Candida fungi (showed inhibition zone 16 mm, 11 mm using Hydra helix and Beta vulgaris, respectively).

scholarly journals The Effects of Copper Addition on The Structure and Antibacterial Properties of Biomedical Glasses

2020 ◽  
Author(s):  
Leyla Mojtabavi ◽  
Amir Razavi
Keyword(s):  
Antibacterial Properties ◽  
Inhibition Zone ◽  
X Ray Diffraction ◽  
Network Modifier ◽  
X Ray ◽  
E Coli ◽  
Copper Addition ◽  
Xrd Patterns ◽  
Copper Incorporation ◽  
Glass Compositions

AbstractIn this work, we studied the effects of copper incorporation in the composition of bioactive glass. Three different glass compositions were synthesized with 0, 3, and 6 mol% of copper addition. X-Ray Diffraction (XRD) patterns confirmed that an amorphous microstructure was obtained for all three glass compositions. Results from Differential Thermal Analysis (DTA) showed that the copper addition in the glass lowers the glass transition temperature, from 646°C to 590°C when added at 6 mol%. X-ray Photoelectron (XPS) survey and high-resolution scans were performed to study the structural effects of copper addition in the glass. Results indicated that the incorporation of copper changes the ratio of bridging to non-birding oxygens in the structure. Glasses were further analyzed for their structure with Nuclear Magnetic Resonance (NMR) spectroscopy, which indicated that copper acts as a network modifier in the glass composition and copper-containing glasses show a less connected microstructure. Antibacterial efficacy of the glasses was analyzed against E. coli and S. epidermis. Copper-containing glasses showed a significantly higher inhibition zone compared to control glass. The glass with 6 mol% copper, exhibited inhibition zones of 9 and 16mm against E. coli and S. epidermis bacteria, respectively.

Characterization of iron oxide nanoparticles from the leaf extract of piper betle by green synthesis method

2020 ◽  
Vol 3 (1) ◽  
Author(s):  
Gowri Manohari N ◽  
Mohanapriya N
Keyword(s):  
Iron Oxide ◽  
Leaf Extract ◽  
Synthesis Method ◽  
Nano Particles ◽  
Piper Betle ◽  
X Ray Diffraction ◽  
Green Method ◽  
X Ray ◽  
Uv Visible

In this present study, Iron Oxide nano particles were synthesized by using Green method. For this synthesis on Iron oxide, the leaf extract of piper betle was used as a reducing agent and FeCl3 as a precursor. Thus, they were characterized by XRD, SEM,EDX and FTIR. The parity of Fe2O3 nano particles was confirmed by EDX. The crystalline size of Iron Oxide nano particles was analyzed using X-ray Diffraction (XRD) spectrum. The functional groups are identified in Fourier Transform Infrared Spectroscopy (FTIR). The surface morphology of the Iron Oxide Nano particles is found from Scanning Electron Microscopy (SEM). The optical properties are determined by using UV-Visible Spectroscopy. Thus, the so-formed nano particles were Fe2O3.

Bio-Synthesis and Characterization of CS–Ag Nano Hydrogel for Antibacterial Application

2020 ◽  
Vol 12 (4) ◽  
pp. 542-547
Author(s):  
Shikha Sharma ◽  
Fehmeeda Khatoon
Keyword(s):  
Ag Nanoparticles ◽  
Spectroscopic Method ◽  
Inhibition Zone ◽  
Plant Leaves ◽  
X Ray Diffraction ◽  
X Ray ◽  
E Coli ◽  
Optimum Wavelength ◽  
Xrd Method

An environmental technique of herbal mediated blend of Ag nanoparticles is a substantial stage in the ground of nanotechnology. Chitosan (CS) is a polymer which is biocompatible and antibacterial. In this work, we have synthesized CS–Ag Nano hydrogel which is form with chitosan-based hydrogel merged in the herbal synthesized Ag nanoparticles. These green synthesized Ag-nanoparticles made from Polygonum bistorta plant leaves and described with the assistance of UV-vis spectrophotometer, and Dynamic Light Scattering (DLS). In this work our main focus to synthesized CS–Ag Nano hydrogel. These hydrogel was described by Fourier transform infrared (FTIR) spectroscopic method, X-ray diffraction (XRD) method, and contact angle. Nanoparticle size distribution was within 1 to 100 nm by DLS and the optimum wavelength was noted in 400 to 450 nm by UV-vis spectroscopic readings. A good antibacterial behavior has been displayed by these synthesized CS–Ag Nano hydrogel films against both E. coli (gram –ve) and S. aureus (gram +ve) with the maximum 7 mm inhibition zone.

scholarly journals Microwave-Assisted Rapid Synthesis of Eu(OH)3/RGO Nanocomposites and Enhancement of Their Antibacterial Activity against Escherichia coli

Materials ◽  
2021 ◽  
Vol 15 (1) ◽  
pp. 43
Author(s):  
Kun-Yauh Shih ◽  
Shiou-Ching Yu
Keyword(s):  
Antibacterial Activity ◽  
Inhibition Zone ◽  
X Ray Diffraction ◽  
Inhibition Zone Diameter ◽  
X Ray ◽  
E Coli ◽  
Inhibition Effect ◽  
Microwave Assisted ◽  
High Antibacterial Activity ◽  
The Eu

Nanomaterials with high antibacterial activity and low cytotoxicity have attracted extensive attention from scientists. In this study, europium (III) hydroxide (Eu(OH)3)/reduced graphene oxide (RGO) nanocomposites were synthesized using a rapid, one-step method, and their antibacterial activity against Escherichia coli (E. coli) was investigated using the synergistic effect of the antibacterial activity between Eu and graphene oxide (GO). The Eu(OH)3/RGO nanocomposites were prepared using a microwave-assisted synthesis method and characterized using X-ray diffraction, scanning electron microscopy, photoluminescence spectroscopy, Raman spectroscopy, and Fourier-transform infrared spectroscopy. Raman sprectroscopy and X-ray diffraction confirmed the pure hexagonal phase structure of the nanocomposites. Further, the antibacterial properties of Eu(OH)3/RGO were investigated using the minimum inhibitory concentration assay, colony counting method, inhibition zone diameter, and optical density measurements. The results revealed that the Eu(OH)3/RGO exhibited a superior inhibition effect against E. coli and a larger inhibition zone diameter compared to RGO and Eu(OH)3. Further, the reusability test revealed that Eu(OH)3/RGO nanocomposite retained above 98% of its bacterial inhibition effect after seven consecutive applications. The high antibacterial activity of the Eu(OH)3/RGO nanocomposite could be attributed to the release of Eu3+ ions from the nanocomposite and the sharp edge of RGO. These results indicated the potential bactericidal applications of the Eu(OH)3/RGO nanocomposite.

Fabrication of PVA Fibers Loaded with Fe3O4 Nano Particles Using Electrospinner

2015 ◽  
Vol 1123 ◽  
pp. 237-240
Author(s):  
Harsojo ◽  
Anita Fira Waluyo ◽  
Harini Sosiati ◽  
Kuwat Triyana
Keyword(s):  
Electron Microscope ◽  
Electrospinning Process ◽  
Nano Particles ◽  
X Ray Diffraction ◽  
Electrospinning Technique ◽  
X Ray ◽  
Nano Powder ◽  
Transmission Electron ◽  
Syringe Needle ◽  
Size Of Particles

It has been studied the fabrication of a sheet made of polyvinyl alcohol (PVA) fibers loaded with Fe3O4 nanomagnetic materials using an electrospinning technique. The solution for the electrospinner was prepared by stirring nano powder of Fe3O4 with a solution of 10% PVA in water at room temperature. The electrospinning process was done under fixed of 15 kV DC voltage, using 12 cm distance between the electrodes, and using a syringe needle having a diameter of 0.4 mm. The morphology of fibers was tested using scanning electron microscope (SEM), while the size of particles was tested using transmission electron microscope (TEM). Moreover, the crystallinity of fibers was tested using Laue and x-ray diffraction, while the magnetic moment was tested using a vibrating sample magnetometer (VSM). The result shows that the PVA sheet composing of fibers having a diameter in the range of 130 mm to 250 mm, accommodating Fe3O4 nano particles in the range of 15 mm to 25 mm, indicating a partial superparamagnetic property.

Biosynthesis of Iron and Cobalt Nanoparticles Using Garlic (Allium cativum) Extract and their Some Applications

2021 ◽  
Vol 882 ◽  
pp. 165-170
Author(s):  
Wisam J. Aziz ◽  
Marwa Abdul Muhsien Hassan ◽  
Mohammed Jwad Khadam
Keyword(s):  
Pathogenic Bacteria ◽  
Chemical Method ◽  
Cobalt Nanoparticles ◽  
Nano Particles ◽  
Cubic Phase ◽  
Oxidizing Agent ◽  
X Ray Diffraction ◽  
X Ray ◽  
E Coli ◽  
Jcpds Card

It has been employed nano particles using garlic (Allium cativum) extract at conditions (pH=12, concentration = 0.1 M, temperature = 90 °C, time =3 hr. and Oxidizing agent (hydrozoan). X-Ray diffraction of Co NPs using Allium cativum with polycrystalline as compared to specification (JCPDS Card no. 030-0443) and the structure is cubic phase and showed nothing at any article than to extract used as search (chemical) method with the outfit that successful chemical picas distinct and no trace of moisture dust concentrations are enlargement were appropriate for the sample. XRD of Fe NPs using Allium cativum is polycrystalline depend on (JCPDS Card no. 052-0513) with peaks (111, 200, 220) and 2θ(42.7, 49.7, 73.1). FESEM pictures of cobalt and Iron nano particles. The nano particles morphology demonstrates irregular, cubic and hexagonal pattern of different sizes which are agglomerated. Other findings with larger magnifications show that all these pictures have soft surfaces. Co NPs / Fe NPs / c-Si n-type (100) structure has a better performance against Co NPs/Si n-type (100) and Fe NPs /Si n-type (100) structures, such that the output of solar cells efficiencies are 6.31, 7.03 and 7.8 % respectively. Cobalt and iron nanostructures produced naturally used to have anti bacterial behavior against pathogenic bacteria such as B-cereus and E-coli with (37, 39) mm, respectively.

Ribosome Structural Organization

1974 ◽  
Vol 32 ◽  
pp. 332-333
Author(s):  
James A. Lake
Keyword(s):  
Ribosomal Proteins ◽  
Structural Organization ◽  
Three Dimensional ◽  
Small Subunit ◽  
Structural Studies ◽  
X Ray Diffraction ◽  
Specific Antibodies ◽  
X Ray ◽  
E Coli ◽  
Complete Sequences

The understanding of ribosome structure has advanced considerably in the last several years. Biochemists have characterized the constituent proteins and rRNA's of ribosomes. Complete sequences have been determined for some ribosomal proteins and specific antibodies have been prepared against all E. coli small subunit proteins. In addition, a number of naturally occuring systems of three dimensional ribosome crystals which are suitable for structural studies have been observed in eukaryotes. Although the crystals are, in general, too small for X-ray diffraction, their size is ideal for electron microscopy.

RNA polymerase: Preparation and structural analysis of helical polymers

1984 ◽  
Vol 42 ◽  
pp. 152-153
Author(s):  
E. Loren Buhle ◽  
Pamela Rew ◽  
Ueli Aebi
Keyword(s):  
Structural Analysis ◽  
Rna Polymerase ◽  
Molecular Level ◽  
X Ray Diffraction ◽  
Helical Polymers ◽  
X Ray ◽  
E Coli ◽  
The Past ◽  
Ordered Arrays ◽  
Low Ionic Strength

While DNA-dependent RNA polymerase represents one of the key enzymes involved in transcription and ultimately in gene expression in procaryotic and eucaryotic cells, little progress has been made towards elucidation of its 3-D structure at the molecular level over the past few years. This is mainly because to date no 3-D crystals suitable for X-ray diffraction analysis have been obtained with this rather large (MW ~500 kd) multi-subunit (α2ββ'ζ). As an alternative, we have been trying to form ordered arrays of RNA polymerase from E. coli suitable for structural analysis in the electron microscope combined with image processing. Here we report about helical polymers induced from holoenzyme (α2ββ'ζ) at low ionic strength with 5-7 mM MnCl2 (see Fig. 1a). The presence of the ζ-subunit (MW 86 kd) is required to form these polymers, since the core enzyme (α2ββ') does fail to assemble into such structures under these conditions.

A New Polyethylene Corrosion Protecting Coating with Ion Selectivity

2011 ◽  
Vol 314-316 ◽  
pp. 273-278
Author(s):  
Yu Hua Dong ◽  
Ke Ren ◽  
Qiong Zhou
Keyword(s):  
Ion Selectivity ◽  
Nano Particles ◽  
Ammonium Bromide ◽  
Linear Low Density Polyethylene ◽  
X Ray Diffraction ◽  
Sulfosalicylic Acid ◽  
X Ray ◽  
Chemically Modified ◽  
Before And After ◽  
Industrial Standards

Linear low density polyethylene (LLDPE) was chemically modified with grafting maleic anhydride (MAH) monomer on its backbone by melting blending. Nano-particles SiO2 was modified by cationic surfactant hexadecyl trimethyl ammonium bromide (CTAB) and anionic surfactant sulfosalicylic acid (SSA) and added to PE coating respectively. Measurement of membrane potential showed that the coating containing modified SiO2 nano-particles had characteristic of ion selectivity. The properties of the different coatings were investigated according to relative industrial standards. Experimental results indicated that PE coating with ion selectivity had better performances, such as adhesion strength, cathodic disbonding and anti-corrosion, than those of coating without ion selectivity. Crystal structure of the coatings before and after alkali corrosion was characterized by Fourier transform infrared spectra (FTIR) and X-ray diffraction (XRD). Structure of the coating without ion selectivity was damaged by NaOH alkali solution, causing mechanical properties being decreased. And the structure of the ion selective coatings was not affected.

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