Investigation of biological activity of recultivated iron ore processing wastes of Abagur agglomeration and processing plant, Novokuznetsk

2021 ◽  
Vol 77 (6) ◽  
pp. 711-716
Author(s):  
M. A. Zakharova ◽  
A. S. Vodoleev ◽  
K. I. Domnin ◽  
S. Yu. Klekovkin
Keyword(s):  
Enzymatic Activity ◽  
Higher Plants ◽  
Maximum Activity ◽  
Soil Microflora ◽  
Plant Residues ◽  
Processing Plant ◽  
Vital Activity ◽  
Tailing Dump ◽  
Degrading Bacteria ◽  
Favorable Conditions

The problem of biotransformation of man-caused disturbed lands is of great importance for the restoration of soil fertility. The leading role in this process is played by soil microorganisms. Toxic industrial waste can make negative adjustments to the species composition of soil microflora (can cause mutation of many microorganisms, as well as the death of some of them). Under certain conditions, in the presence of harmful substances, new microorganisms can arise that decompose precisely those substances that cause soil pollution. Consequently, a new microflora appears, capable of carrying out its trophic functions on an inductive basis on industrially contaminated substrates. As a man-caused control, a mineral substrate of the tailing dump of Abagurskaya agglomeration and processing plant JSC, unfavorable for the vital activity of soil microflora, where, due to the absence of plant residues and other carbon-containing components, an extremely low level of intensity of enzymatic hydrolytic processes was used. The introduction of organic matter containing sewage sludge (WWS) into the substrate as a recultivator creates favorable conditions for the formation of a stable vegetation cover. The presence of an organic component in the mineral substrate of the iron-containing tailings waste creates favorable conditions for the vital activity of microflora. Carbon-containing compounds of technozems create a certain pool of microorganisms. The products of their vital activity create the basis for the formation of an organic-mineral substrate, favorable conditions for the growth of higher plants and the creation of a stable phytocenosis. For the induced manifestation of enzymatic activity under these extreme conditions, a considerable time is required for the bacterial forms introduced from the outside to be able to fix their presence. The maximum activity, comparable both after 1 month and after 3 months with the natural control, was noted on the experimental site with the placement of WWS as a soil conditioner. The presence of WWS and intensive accumulation of leaf litter and other plant residues contribute to the favorable development of cellulose-degrading bacteria, among which rod-shaped forms of the genus Clostridium predominate. The quantitative indicators of the enzymatic activity of technozems are close to the natural level and significantly exceed those of technogenic control.

scholarly journals DEGRADATION OF KERATIN CONTAINING WASTES BY BACTERIA WITH KERATINOLYTIC ACTIVITY

2015 ◽  
Vol 1 ◽  
pp. 284 ◽  
Author(s):  
Veslava Matikevičienė ◽  
Danutė Masiliūnienė ◽  
Saulius Grigiškis
Keyword(s):  
Maximum Level ◽  
Maximum Activity ◽  
Processing Plant ◽  
Bacterial Strains ◽  
Keratinolytic Activity ◽  
Cultivation Time ◽  
Good Ability ◽  
Degrading Bacteria ◽  
Poultry Processing ◽  
Wastewater Bacteria

The aim of this study was to select keratin-degrading bacteria from JSC “Biocentras” collection and poultry processing plant wastewater, and to study their ability to degrade chicken feathers. Isolated from poultry processing plant wastewater bacteria was grown in basal media with feathers meal and showed high keratinolytic activity and protein content throughout the cultivation time. Bacterial strains B. licheniformis 511, B. subtilis I1, B. subtilis 717, and B. subtilis 103 suggested strongly of bacteria that produces keratinolytic activity in the cell free culture supernatants. The obtained results showed that maximum activity of keratinase is a function of cultivation time by the bacteria tested. B. subtilis 103 reached to its maximum level of keratinase production (152 U/mL) after 24 hrs, when over bacteria (148-242 U/mL) after 48 hrs. The good ability of selected bacteria to degrade feathers was detected. The best biodegradation of feathers was obtained using B. subtilis I-1. Over bacillus good degraded feathers as well.

THE EFFECT OF DECOMPOSITION OF VARIOUS CROP PLANTS ON THE METABOLIC ACTIVITY OF THE SOIL MICROFLORA

1962 ◽  
Vol 8 (4) ◽  
pp. 501-509 ◽  
Author(s):  
I. L. Stevenson
Keyword(s):  
Oxygen Uptake ◽  
Metabolic Activity ◽  
Respiratory Activity ◽  
Experimental Period ◽  
Maximum Activity ◽  
Soil Microflora ◽  
Plant Residues ◽  
Rapid Decline ◽  
Decomposition Of Plant Residues

Studies were undertaken to determine the changes in the soil microflora during the decomposition of plant residues. The methods used include the determination of bacterial numbers, dehydrogenase activity, and oxygen uptake with selected substrates (ethanol, amino acids, organic acids). Tests were conducted at varying intervals during a 66-day experimental period. Maximum activity was observed in all supplemented soils during the early stages of decomposition (5–10 days) followed by a rapid decline. During the first 10 days a correlation was found between numbers of microorganisms and the respiratory activity (oxygen uptake) of the soils. Analysis of the data from this period indicated that the increased metabolic activity of the soil was due partially to a preferential stimulation of the organisms and not to the effects of increased numbers alone. Nutritional grouping of bacteria isolated from the flax supplemented soil at 0 and after 6 days of decomposition indicated a shift in the microbial equilibrium, towards a higher proportion of bacteria with relatively simple nutritional requirements.

scholarly journals Effect of pH on the Electrochemical Behavior of Hydrogen Peroxide in the Presence of Pseudomonas aeruginosa

2021 ◽  
Vol 9 ◽  
Author(s):  
Javier Espinoza-Vergara ◽  
Paulo Molina ◽  
Mariana Walter ◽  
Miguel Gulppi ◽  
Nelson Vejar ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Pseudomonas Aeruginosa ◽  
Enzymatic Activity ◽  
Electrochemical Behavior ◽  
Electrochemical Techniques ◽  
Stable State ◽  
Reduction Reaction ◽  
Maximum Activity ◽  
Open Circuit ◽  
Mueller Hinton

The influence of pH on the electrochemical behavior of hydrogen peroxide in the presence of Pseudomonas aeruginosa was investigated using electrochemical techniques. Cyclic and square wave voltammetry were used to monitor the enzymatic activity. A modified cobalt phthalocyanine (CoPc) carbon electrode (OPG), a known catalyst for reducing O2 to H2O2, was used to detect species resulting from the enzyme activity. The electrolyte was a sterilized aqueous medium containing Mueller-Hinton (MH) broth. The open-circuit potential (OCP) of the Pseudomonas aeruginosa culture in MH decreased rapidly with time, reaching a stable state after 4 h. Peculiarities in the E / I response were observed in voltammograms conducted in less than 4 h of exposure to the culture medium. Such particular E/I responses are due to the catalase’s enzymatic action related to the conversion of hydrogen peroxide to oxygen, confirming the authors’ previous findings related to the behavior of other catalase-positive microorganisms. The enzymatic activity exhibits maximum activity at pH 7.5, assessed by the potential at which oxygen is reduced to hydrogen peroxide. At higher or lower pHs, the oxygen reduction reaction (ORR) occurs at higher overpotentials, i.e., at more negative potentials. In addition, and to assess the influence of bacterial adhesion on the electrochemical behavior, measurements of the bacterial-substrate metal interaction were performed at different pH using atomic force microscopy.

Diffusion-Linked Microbial Metabolism in the Vadose Zone

1999 ◽  
Author(s):  
J. E. Watson ◽  
R. F. Harris
Keyword(s):  
Vadose Zone ◽  
Plant Biomass ◽  
Higher Plants ◽  
High Energy ◽  
Essential Elements ◽  
Biofilm Reactor ◽  
Plant Residues ◽  
Soil Organisms ◽  
Transport And Transformation ◽  
Low Energy Electrons

Figure 7.1 is a schematic of nutrient and contaminant transformations and cycling in the vadose zone. As detailed in Harris and Arnold (1995), higher plants take up C, N, P, and S in their most oxidized forms and use, via photosynthesis, the Sun’s energy and low-energy electrons from the oxygen in water to convert the oxidized forms of these essential elements into the relatively high energy reduced forms comprising plant biomass. Following plant death, the biomass residues enter the soil and are attacked by soil organisms as a source of food. The plant residues are depolymerized and the reduced, high-energy monomers are assimilated in part into soil organism biomass, and in part are used as electron donors to combine with the most thermodynamically efficient electron acceptors for dissimilatory energy generation to drive growth and maintenance reactions. In aerobic zones, oxygen is the preferred electron acceptor as long as it is nonlimiting. Death of soil organisms produces dead biomass which re-enters the biological reactor. Ultimately, via respiration in aerobic soils, all the reduced C, N, P, and S materials are released as their oxidized forms, and oxygen is reduced to water to complete the cycle. Ideally, the cycle is conservative, particularly from the standpoint of nonleakage of nutrients, such as nitrate, into the groundwater. Similarly, contaminants entering the vadose zone, either as a function of agronomic use or by accident, should ideally be integrated into the natural nutrient cycles and converted to harmless by-products for assimilation and dissimilation by soil organisms and higher plants (Liu, 1994). Management of nutrient and contaminant transformations by the soil organisms requires a thorough understanding of the ecophysiological and solute transport ground rules that control the nature and rates of transformation options available to the soil organisms. In models of chemical transport and transformation through the vadose zone, colonies of microorganisms are frequently treated as a homogeneous biofilm reactor (Grant and Rochette, 1994). Often, modeling efforts are focused on environmental conditions external to the microbial colony. This consideration of the colony as a biofilm with relatively constant nutrient uptake rates ignores the growth differentiation that occurs as the colony develops

scholarly journals Sugarcane bagasse as a source of carbon for enzyme production by filamentous fungi1

Hoehnea ◽  
2018 ◽  
Vol 45 (1) ◽  
pp. 134-142 ◽  
Author(s):  
Flaviane Lopes Ferreira ◽  
Cesar Barretta Dall'Antonia ◽  
Emerson Andrade Shiga ◽  
Larissa Juliani Alvim ◽  
Rosemeire Aparecida Bom Pessoni
Keyword(s):  
Carbon Source ◽  
Enzymatic Activity ◽  
Sugarcane Bagasse ◽  
Enzyme Production ◽  
Sole Carbon Source ◽  
Sole Source ◽  
Maximum Activity ◽  
Liquid Media ◽  
Biotechnological Potential ◽  
Efficient Producer

ABSTRACT The aim of the present work was to assess the enzymatic activity of six strains of filamentous fungi grown in liquid media containing 1% sugarcane bagasse as the sole carbon source. All fungal strains were able to use this agro-industrial residue, producing various types of enzymes, such as cellulases, xylanases, amylases, pectinases, and laccases. However, Aspergillus japonicus Saito was the most efficient producer, showing the highest enzymatic activity for laccase (395.73 U L-1), endo-β-1,4-xylanase (3.55 U mL-1) and β-xylosidase (9.74 U mL-1) at seven, fourteen and twenty-one days in culture, respectively. Furthermore, the endo-β-1,4-xylanases and β-xylosidases of A. japonicus showed maximum activity at 50°C, and pH 5.5 and pH 3.5-4.5, respectively. Thus, these results indicate that A. japonicus has a great biotechnological potential for the production of these enzymes using sugarcane bagasse as the sole source of carbon.

scholarly journals UDP-sugar Pyrophosphorylase with Broad Substrate Specificity Toward Various Monosaccharide 1-Phosphates from Pea Sprouts

2004 ◽  
Vol 279 (44) ◽  
pp. 45728-45736 ◽  
Author(s):  
Toshihisa Kotake ◽  
Daisuke Yamaguchi ◽  
Hiroshi Ohzono ◽  
Sachiko Hojo ◽  
Satoshi Kaneko ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Amino Acids ◽  
Substrate Specificity ◽  
Molecular Mass ◽  
Glucuronic Acid ◽  
De Novo ◽  
Higher Plants ◽  
Maximum Activity ◽  
Broad Substrate Specificity ◽  
Salvage Pathways

UDP-sugars, activated forms of monosaccharides, are synthesized throughde novoand salvage pathways and serve as substrates for the synthesis of polysaccharides, glycolipids, and glycoproteins in higher plants. A UDP-sugar pyrophosphorylase, designated PsUSP, was purified about 1,200-fold from pea (Pisum sativumL.) sprouts by conventional chromatography. The apparent molecular mass of the purified PsUSP was 67,000 Da. The enzyme catalyzed the formation of UDP-Glc, UDP-Gal, UDP-glucuronic acid, UDP-l-arabinose, and UDP-xylose from respective monosaccharide 1-phosphates in the presence of UTP as a co-substrate, indicating that the enzyme has broad substrate specificity toward monosaccharide 1-phosphates. Maximum activity of the enzyme occurred at pH 6.5–7.5, and at 45 °C in the presence of 2 mmMg2+. The apparentKmvalues for Glc 1-phosphate andl-arabinose 1-phosphate were 0.34 and 0.96 mm, respectively.PsUSPcDNA was cloned by reverse transcriptase-PCR.PsUSPappears to encode a protein with a molecular mass of 66,040 Da (600 amino acids) and possesses a uridine-binding site, which has also been found in a human UDP-N-acetylhexosamine pyrophosphorylase. Phylogenetic analysis revealed that PsUSP can be categorized in a group together with homologues fromArabidopsisand rice, which is distinct from the UDP-Glc and UDP-N-acetylhexosamine pyrophosphorylase groups. Recombinant PsUSP expressed inEscherichia colicatalyzed the formation of UDP-sugars from monosaccharide 1-phosphates and UTP with efficiency similar to that of the native enzyme. These results indicate that the enzyme is a novel type of UDP-sugar pyrophosphorylase, which catalyzes the formation of various UDP-sugars at the end of salvage pathways in higher plants.

EFFECT OF CERTAIN CATIONS ON ACTIVITY OF LEUCINE NAPHTHYLAMIDASES OF PARAMECIUM CAUDATUM

1967 ◽  
Vol 13 (9) ◽  
pp. 1133-1138 ◽  
Author(s):  
Norvell W. Hunter
Keyword(s):  
Enzymatic Activity ◽  
Maximum Activity ◽  
Paramecium Caudatum ◽  
Optimum Activity ◽  
Spectrophotometric Methods

Spectrophotometric methods were used to study the activity of the leucine naphthylamidases of Paramecium caudatum. Optimum activity of the enzymes occurred from pH 6.6 to 6.8 and was enhanced by either Co2+, Mn2+, or by Mg2+. Maximum activity occurred when Co2+ was used with either Mg2+ or Mn2+. Activity was depressed by either Sr2+, Fe2+, Zn2+, Ni2+, Hg2+, Cd2+, Pb2+, Sn2+, or Li2+, and not affected by Ba2+, Cu2+, and Ca2+. Approximately 40% of the enzymatic activity was inhibited by EDTA. Most of this inhibition was reversed by either Mg2+, Co2+, or Mn2+.

scholarly journals EXPERIMENTAL ASSESSMENT OF TOXIC EFFECTS OF TROTYL (TNT) ON SOIL MICROFLORA

2018 ◽  
pp. 34-38
Author(s):  
A. A. Maslennikov ◽  
S. A. Demidova
Keyword(s):  
Health Hazard ◽  
Subsequent Development ◽  
Soil Microflora ◽  
Vital Activity ◽  
Experimental Assessment ◽  
Soil Mixture ◽  
E Coli ◽  
Significant Growth ◽  
Hygienic Standard ◽  
Test Organisms

An experimental assessment of the hazard of trotyl content in the soil has been carried out according to one of the most significant sanitary indicators of harm - the microbiocenosis. E. coli, micromycetes, actinomycetes and saprofitny bacteria grown up in the specific to each microflora medium were used as test organisms. It was found that the content of the toxicant in the soil at the levels of 20.0 and 10.0 mg/kg during the experiment revealed a significant growth of E. coli (up to 458.87 % relative to the control) indicating the inhibition of the process of self-purification of the soil. In addition, TNT in these concentrations contributed to a significant growth of micromycete colonies (up to 200.00%) indicating its accumulation in the soil, where fungi act as indicators (toxin accumulators). At the same time the substance at the level of 20.0 mg/kg caused inhibition of vital activity of actinomycetes reaching 50.27 %. The number of colonies of saprophytic microflora also significantly decreased in the soil mixture with the content of the chemical at the levels of 20.0 and 10.0 mg/kg. According to this type of general health hazard indicator the concentration of TNT – 10.0 mg/kg has been accepted as a threshold, and the value of 2.0 mg/kg – as inactive. The data obtained are taken into account in the subsequent development of the hygienic standard for this explosive compound.

scholarly journals An Active of Extracellular Cellulose Degrading Enzyme from Termite Bacterial Endosimbiont

2016 ◽  
Vol 20 (1) ◽  
pp. 62
Author(s):  
M. Saifur Rohman ◽  
Endang Pamulatsih ◽  
Yudi Kusnadi ◽  
Triwibowo Yuwono ◽  
Erni Martani
Keyword(s):  
Enzymatic Activity ◽  
Maximum Activity ◽  
Enzymatic Activities ◽  
Enzymatic Assay ◽  
Glycosidic Bond ◽  
Acidic Ph ◽  
Total Proteins ◽  
Degrading Enzyme ◽  
Paenibacillus Sp ◽  
Simple Carbohydrate

Cellulase is an ezyme that specifically cleaves the 1,4-β-glycosidic bond of cellulose to produce thesmall fragments of simple carbohydrate. This work was aimed to characterize the extracellular cellulase fromPaenibacillus spp., which was previously isolated from macro termites, Odontotermes bhagwatii in our laboratory.Two Paenibacillus isolates were used in this experiment, namely Paenibacillus cellulositrophicus SBT1 andPaenibacillus, sp. SBT8. Analysis of the total proteins in the supernatants showed that P. cellulositrophicus SBT1and Paenibacillus sp. SBT8 roughly produced as much as 18.6 mg/l and 24.8 mg/l of extracellular cellulases,respectively. Enzymatic assay showed that SBT1 and SBT8 cellulase exhibited enzymatic acitivity of 0.17 U/mg and 0.12 U/mg, respectively. Temperature dependencies analysis indicated that both cellulases exhibitedmaximum activity at 35oC. At the temperature higher than 55oC, the enzymatic activities of both cellulases wereroughly 20% reduced compared to the maximum activity. SBT1 and SBT8 cellulases were both active at acidicpH. At basic pH (pH 8) the enzymatic activities of both cellulases were reduced roughly 30% compared to thatof acidic pH. Supplementing of Mg2+, Zn2+, and Ca2+ in range of 1-10 mM increased the enzymatic activity ofboth cellulases roughly 33 to 50%.

scholarly journals ANALYSIS OF THE POWER BALANCE OF THE TECHNOLOGICAL SYSTEM FOR GRINDING AGRICULTURAL PLANT WASTE

2020 ◽  
pp. 122-128
Author(s):  
Ihor Kupchuk ◽  
Oleksii Tokarchuk ◽  
Volodymyr Hontar ◽  
Andrii Didyk
Keyword(s):  
Energy Consumption ◽  
Energy Balance ◽  
High Energy ◽  
Raw Material ◽  
Technological System ◽  
Plant Residues ◽  
Processing Plant ◽  
Industrial Complex ◽  
Plant Origin ◽  
The Government

Despite some decisions approved by the Government of Ukraine to address the problems of efficient and environmentally sound solid waste management, in particular those that accumulate as a result of economic activity of the agro-industrial complex of the state, their practical implementation of measures declared by the Government is currently low. levels. Among a number of deterrents, the main ones include: lack of centralized recycling facilities and economically accessible for small facilities technical and technological base of waste processing, ineffective environmental control and the necessary management practices. However, vegetable waste is not always used, although it is a valuable raw material that can be used as a raw material for the production of fuel pellets, which will significantly reduce the energy dependence of the enterprise. An integral part of the technological cycle of processing plant residues into fuel briquettes is the preparation of biomass for briquetting in order to ensure the required particle size. Given the strategic importance of the formation of material and technical base to ensure highly efficient processing of agricultural waste of plant origin, as well as high energy consumption, which marks the traditional preparation of biomass for briquetting, there is a need for research to solve the problem of energy efficient grinding of structurally heterogeneous materials. high moisture content, which determines the relevance of the article. Thus, in order to achieve high efficiency of the process of grinding waste of plant origin, promising ways to reduce the energy consumption of this process based on the energy balance of the vibratory crusher rotor type and analysis of relationships between elements of the structural block diagram of the energy balance of the technological system "Vibration-rotor crusher - the processed environment ".

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