The problem of biotransformation of man-caused disturbed lands is of great importance for the restoration of soil fertility. The leading role in this process is played by soil microorganisms. Toxic industrial waste can make negative adjustments to the species composition of soil microflora (can cause mutation of many microorganisms, as well as the death of some of them). Under certain conditions, in the presence of harmful substances, new microorganisms can arise that decompose precisely those substances that cause soil pollution. Consequently, a new microflora appears, capable of carrying out its trophic functions on an inductive basis on industrially contaminated substrates. As a man-caused control, a mineral substrate of the tailing dump of Abagurskaya agglomeration and processing plant JSC, unfavorable for the vital activity of soil microflora, where, due to the absence of plant residues and other carbon-containing components, an extremely low level of intensity of enzymatic hydrolytic processes was used. The introduction of organic matter containing sewage sludge (WWS) into the substrate as a recultivator creates favorable conditions for the formation of a stable vegetation cover. The presence of an organic component in the mineral substrate of the iron-containing tailings waste creates favorable conditions for the vital activity of microflora. Carbon-containing compounds of technozems create a certain pool of microorganisms. The products of their vital activity create the basis for the formation of an organic-mineral substrate, favorable conditions for the growth of higher plants and the creation of a stable phytocenosis. For the induced manifestation of enzymatic activity under these extreme conditions, a considerable time is required for the bacterial forms introduced from the outside to be able to fix their presence. The maximum activity, comparable both after 1 month and after 3 months with the natural control, was noted on the experimental site with the placement of WWS as a soil conditioner. The presence of WWS and intensive accumulation of leaf litter and other plant residues contribute to the favorable development of cellulose-degrading bacteria, among which rod-shaped forms of the genus Clostridium predominate. The quantitative indicators of the enzymatic activity of technozems are close to the natural level and significantly exceed those of technogenic control.
DEGRADATION OF KERATIN CONTAINING WASTES BY BACTERIA WITH KERATINOLYTIC ACTIVITY
