scholarly journals CHANGES OF MORPHOFUNCTIONAL STATE OF THYROID GLAND IN HYPOTHYROID RATS IN CONDITIONS OF INFLUENCE LONG-TERM STRESS

2019 ◽  
Vol 15 (1-2) ◽  
pp. 20-24
Author(s):  
N.G. Pobigun
Keyword(s):  
Electron Microscopy ◽  
Thyroid Gland ◽  
Blood Plasma ◽  
Light And Electron Microscopy ◽  
Structural Features ◽  
Thyroid Stimulating Hormone ◽  
Stress Factors ◽  
Male Rats ◽  
In Blood Plasma

Relevance. The chronic stress influences on increasing of frequency of thyroid pathology together with other factors (decreased iodine intake in food, harmful factors of external environment, high technogenic loading, depravation of general ecological state, violation of structure of nutrition). The changes of hypothalamus-hypophysis-thyroid gland axis at stress is not enough agreed because of influence stressful factors with different character and duration. Objective is study of influence of chronic emotional painful stress (CS) on the changes of morphofunctional state of thyroid gland (TG) on the background of experimental hypothyroidism (EH). Materials and methods. Experiments were performed on 48 white laboratory sex matured male rats. Animals were divided on four groups: the fist – intact animals; the second – animals with EH; the third – animals under influence of CS; the fourth – animals with EH under action of CS. The EH was modeled by everyday addition of mercazolilum (dose 10mg/kg) to drinking water during 15 days. The CS was modeled by 1 hour per day during 30 days. The functional activity of TG was estimated by the concentration of thyroid stimulating hormone (TSH), triiodothyronine (T3), thyroxin (T4) in blood plasma and calculation of thyroid index (TI). The morphological state of TG was studied by light and electron microscopy. The statistical analysis of results was processed using Student’s test with Bonferroni-Holm correction in program Statistica 6.0. The differences were statistical significant at р<0,05.             Results. The long-term influence of stress factors caused significant decreasing of level of thyroid hormones in blood plasma in comparison to intact animals. The data of light and electron microscopy of TG of rats under influence of CS confirm biochemical changes and detect signs of decreasing of secretory function. On the background of action of mercazolilum the long-term stress caused to more substantial changes of functional state of TG (significant increasing of level of TSH, decreasing of content of T4 and TI) in comparison to data of animals with EH. The structural features of TG of animals with EH under influence of CS indicate on suppression of function. Conclusions. Decreasing of synthesis of hormones by TG in rats at conditions of CS was detected. The long-term action of stress on background of hypothyroidism causes to more substantial depravation of thyroid profile.

scholarly journals FREE AMINO ACIDS OF BLOOD PLASMA AS AN INTEGRAL PARAMETER OF METABOLIC DISORDERS IN A LONG-TERM INTAKE OF SMALL DOSES OF LEAD ACETATE INTO THE ORGANISM

2017 ◽  
pp. 67-71
Author(s):  
V. M. Sheybak ◽  
A. Y. Pavliukovets ◽  
V. Yu. Smirnov
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Blood Plasma ◽  
Free Amino Acids ◽  
Free Amino ◽  
Lead Acetate ◽  
Male Rats ◽  
Integral Parameter ◽  
In Blood Plasma

Objective: to analyze the pool of free amino acids and their nitrogen-containing metabolites of blood plasma of animals being administered lead acetate for 21 or 28 days. Material and methods. The experiments were carried out on white male rats with the initial weight of 140-160 g. During the experiment the animals received a total amount of 0.67 g of lead acetate (21 days), the second group - 1.12 g (28 days). The determination of free amino acids in blood plasma was performed by the method of reversed-phase HPLC. Results. As a result of the research it has been found out that the most significant contribution to the formation of the amino acid imbalance in blood plasma as a result of the long-term intake of lead acetate according to the value of the Fisher test is caused by changes in the concentrations of α-aminobutyric acid, isoleucine, aspartate, arginine, glycine, serine, histidine, and methionine. Conclusion. The long-term intake of lead acetate with drinking water leads to a statistically significant increase of general content of free amino acids and their nitrogen-containing metabolites, as well as to a decrease of the concentration the essential amino acid threonine in blood plasma.

scholarly journals INFLUENCE OF ALIMENTARY INSUFFICIENCY OF CALCIUM, MAGNESIUM AND IODINE ON RAT VITAMIN STATUS

2021 ◽  
Vol 22 (1) ◽  
pp. 52-59
Author(s):  
O.A. Vrzhesinskaya ◽  
◽  
V.M. Kodentsova ◽  
N.A. Beketova ◽  
O.V. Kosheleva ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Vitamin B1 ◽  
Male Rats ◽  
Molar Ratio ◽  
Vitamin Status ◽  
Iron And Zinc ◽  
Calcium Magnesium ◽  
Increased Activity ◽  
Experimental Group ◽  
In Blood Plasma

The diet of the majority of the population of Russia is characterized by an insufficient content of a number of micronutrients, including macro- and micronutrients. The aim of the study is to characterize in a rat model experiment the effect of combined nutritional deficiency of calcium, magnesium and iodine on the availability of other mineral substances, vitamin metabolism and biochemical parameters blood plasma. The lack of minerals in growing Wistar male rats with an initial body weight (51.4 ± 0.5) g was created for 23 days by halving of a calcium, magnesium and iodine in the salt mixture. Alimentary deficiency of calcium, magnesium and iodine in growing rats significantly and multidirectionally affected the metabolism of other minerals and vitamins, as well as a number of diagnostically significant indicators of blood and urine. In rats of the experimental group, the liver concentration of α-tocopherol, vitamin B1 and retinol palmitate decreased, the concentration of iron and zinc increased, the copper content in the whole brain decreased significantly. The molar ratio of γ- and α-tocopherols in blood plasma was increased, the concentration of the circulating form of vitamin D decreased. The ratio of AlAT / AsAT (de Ritis coefficient) markedly decreased due to increased activity of AlAT. Phosphate reabsorption decreased slightly. Increased urinary excretion of riboflavin was accompanied by a decrease in its concentration in blood plasma. The conclusion that insufficient intake of calcium, magnesium and iodine may disturb vitamin-mineral status of organism has been made.

scholarly journals Role of fibronectin under conditions of doxorubicin action

2015 ◽  
Vol 6 (1) ◽  
pp. 17-22
Author(s):  
A. I. Shevtsova ◽  
G. A. Ushakova
Keyword(s):  
Blood Plasma ◽  
Total Protein ◽  
Protein Concentration ◽  
Male Rats ◽  
Antitumor Action ◽  
Total Protein Concentration ◽  
Monospecific Antibodies ◽  
Anthracycline Chemotherapy ◽  
In Blood Plasma

There is no standard as to treatment of anthracycline chemotherapy complications. The reduction of cytotoxic drugs toxicity without weakening of their antitumor action remains relevant. The extracellular matrix which key component is fibronectin is present in all tissues and it continuously undergoes controlled remodeling. So, the purpose of our work was to study the level of fibronectin in the experimental model of doxorubicin-induced cardiomyopathy and effects of this cytostatic and its co-administration with antioxidants of different nature.The level of fibronectin was measured by ELISA using monospecific antibodies against fibronectin (Sigma, USA), secondary anti-IgG labeled with horseradish peroxidase (Sigma, USA) and fibronectin standard (Sigma, USA). The study was conducted on Wistar male rats with weight of 210 ± 50 g which were divided into 4 groups by 8 animals in each group: 1 – control, rats receiving saline i/p; 2 – doxorubicin 1 mg/kg i/p once a week during 4 weeks; 3 – doxorubicin by the same scheme plus 1% 2-oxoglutarate in drinking water during 4 weeks;4 – doxorubicin by the same scheme and korvitin injection 30 min before doxorubicin application once a week during 4 weeks. Obtained data indicate the effect of doxorubicin to decrease in index mass heart in 38% of animals compared to control animals; decrease in total protein concentration by 8% (Р < 0,05) and increase of the level of fibronectin by 67% (P < 0,001) in blood plasma of rats and decrease in the level of fibronectin in the heart extract by 19% (Р < 0,05) under development of doxorubicin-induced cardiotoxicity. Increased fibronectin concentration in blood plasma had strong correlation with decreased total protein concentration in blood (r=0,80) and heart extract (r=0,59) in rats with doxorubicin-induced cardiomiophaty indicating the sensitive reaction of fibronectin to development of metabolic disorders under doxorubicin influence. 

Trace Element Status (Zinc, Copper, Selenium, Iron, Manganese) in Patients with Long-Term Home Parenteral Nutrition

2016 ◽  
Vol 69 (2) ◽  
pp. 120-124 ◽  
Author(s):  
Milan Dastych Jr. ◽  
Michal Šenkyřík ◽  
Milan Dastych ◽  
František Novák ◽  
Petr Wohl ◽  
...  
Keyword(s):  
Trace Elements ◽  
Parenteral Nutrition ◽  
Blood Plasma ◽  
Whole Blood ◽  
Statistical Significance ◽  
Home Parenteral Nutrition ◽  
Control Group ◽  
The Status ◽  
In Blood Plasma

Background: The objective of the present study was to determine concentrations of zinc (Zn), copper (Cu), iron (Fe), selenium (Se) in blood plasma and manganese (Mn) in the whole blood in patients with long-term home parenteral nutrition (HPN) in comparison to the control group. Patients and Methods: We examined 68 patients (16 men and 52 women) aged from 28 to 68 years on a long-term HPN lasting from 4 to 96 months. The short bowel syndrome was an indication for HPN. The daily doses of Zn, Cu, Fe, Se and Mn in the last 3 months were determined. Results: No significant differences in blood plasma were found for Zn, Cu and Fe in patients with HPN and in the control group (p > 0.05). The concentration of Mn in whole blood was significantly increased in HPN patients (p < 0.0001), while Se concentration in these patients was significantly decreased (p < 0.005). The concentration of Mn in the whole blood of 16 patients with cholestasis was significantly increased compared to the patients without cholestasis (p < 0.001). The Cu concentration was increased with no statistical significance. Conclusion: In long-term HPN, the status of trace elements in the patients has to be continually monitored and the daily substitution doses of these elements have to be flexibly adjusted. Dosing schedule needs to be adjusted especially in cases of cholestatic hepatopathy. A discussion about the optimal daily dose of Mn in patients on HPN is appropriate. For clinical practice, the availability of a substitution mixture of trace elements lacking Mn would be advantageous.

Correlated 3D Light and Electron Microscopy of Large, Complex Structures: Analysis of Transverse Tubules in Heart Failure

1998 ◽  
Vol 4 (S2) ◽  
pp. 440-441
Author(s):  
Maryann E. Martone ◽  
Andrea Thor ◽  
Stephen J. Young ◽  
Mark H. Ellisman.
Keyword(s):  
Electron Microscopy ◽  
Electron Tomography ◽  
Light And Electron Microscopy ◽  
Structural Features ◽  
Electron Microscopic Level ◽  
Specimen Preparation ◽  
Large Sample Size ◽  
Electron Microscopic ◽  
High Voltage Electron Microscopy ◽  
Voltage Electron

Light microscopic imaging has experienced a renaissance in the past decade or so, as new techniques for high resolution 3D light microscopy have become readily available. Light microscopic (LM) analysis of cellular details is desirable in many cases because of the flexibility of staining protocols, the ease of specimen preparation and the relatively large sample size that can be obtained compared to electron microscopic (EM) analysis. Despite these advantages, many light microscopic investigations require additional analysis at the electron microscopic level to resolve fine structural features.High voltage electron microscopy allows the use of relatively thick sections compared to conventional EM and provides the basis for excellent new methods to bridge the gap between microanatomical details revealed by LM and EM methods. When combined with electron tomography, investigators can derive accurate 3D data from these thicker specimens. Through the use of correlated light and electron microscopy, 3D reconstructions of large cellular or subcellular structures can be obtained with the confocal microscope,

Maintenance of differentiated sheep thyroid cells in primary culture for three months

1984 ◽  
Vol 107 (1) ◽  
pp. 54-59 ◽  
Author(s):  
Carmel Mothersill ◽  
C. Seymour ◽  
J. F. Malone
Keyword(s):  
Electron Microscopy ◽  
Sudden Death ◽  
Primary Culture ◽  
Light And Electron Microscopy ◽  
Functional Differentiation ◽  
Entire Period ◽  
Iodine Uptake ◽  
Long Term Effects ◽  
Thyroid Cells

Abstract. A method is described which permits culture of primary thyroid cells without subculture for at least 100 days. Cultures are maintained without medium changes for the entire period, and concentrated glucose is added to replenish energy supplies at carefully defined intervals. The cells retain morphological and functional differentiation shown by light and electron microscopy, PAS positive histochemistry, iodine uptake and T4 production for at least 100 days. After this time fairly sudden death of the cultures occurs. Possible mechanisms for the effect are postulated. The technique should make it possible to study long-term effects of drugs/radiation on differentiated cultures without the need for continuous subculture.

Testicular oxytocin: effects of intratesticular oxytocin in the rat

1991 ◽  
Vol 130 (2) ◽  
pp. 231-NP ◽  
Author(s):  
H. D. Nicholson ◽  
S. E. F. Guldenaar ◽  
G. J. Boer ◽  
B. T. Pickering
Keyword(s):  
Leydig Cells ◽  
Light And Electron Microscopy ◽  
Male Rats ◽  
Plasma Testosterone ◽  
Epididymal Sperm ◽  
Long Term Effects ◽  
Sperm Counts ◽  
Term Administration

ABSTRACT The long-term effects of oxytocin administration on the testis were studied using intratesticular implants. Adult male rats had an Accurel device containing 20 μg oxytocin (releasing approximately 200 ng/day) implanted into the parenchyma of each testis; control animals received empty devices. The animals were killed at weekly intervals for 4 weeks. Some animals were perfused and the testes processed for light and electron microscopy. Blood was collected from the remaining animals for the measurement of testosterone, dihydrotestosterone, LH, FSH and oxytocin; epididymal sperm counts were measured and the testes were extracted and radioimmunoassayed for testosterone, dihydrotestosterone and oxytocin. Long-term administration of oxytocin resulted in a significant reduction in testicular and plasma testosterone levels throughout the 4-week period examined and, after 14 days of treatment, lipid droplets were seen in the Leydig cells of treated but not control animals. Concentrations of dihydrotestosterone in the plasma and testes of the oxytocin-treated animals, however, were significantly elevated after 7 and 14 days and at no time fell below control values. Plasma FSH levels were also lower in the oxytocin-treated animals. Intratesticular oxytocin treatment did not affect LH or oxytocin concentrations in the plasma, epididymal sperm counts or the number of Leydig cells in the testis. Empty Accurel devices had no effect on testicular morphology. This study provides the first evidence that oxytocin in vivo can modify steroidogenesis in the testis. Journal of Endocrinology (1991) 130, 231–238

Mechanisms Controlling Dormancy in the Arid Zone Grass Aristida contorta. II. Anatomy of the Hull

1974 ◽  
Vol 22 (4) ◽  
pp. 647 ◽  
Author(s):  
JJ Mott ◽  
PW Tynan
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Gas Permeability ◽  
Arid Zone ◽  
Light And Electron Microscopy ◽  
Anatomical Change ◽  
Definitive Statement ◽  
Scanning Electron

The anatomy of the hull directly over the embryo was examined by light and electron microscopy in long-term dormant and non-dormant grains of A. contorta. A lipid-containing layer was noted, covering the surface of the inner epidermis of the hull, and examination by scanning electron microscopy showed that although the layer was intact in all dormant grain examined, it was fractured in non-dormant grain. A definitive statement on the function of these cracks is not possible, but they appear to be an anatomical change leading to increased gas permeability of the hull of non-dormant grains.

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