scholarly journals IN VITRO COMPATIBILITY BETWEEN INSECTICIDES AND THE COMMERCIAL BIOINSECTICIDE AGREE® WG

2020 ◽  
Vol 7 (1) ◽  
pp. 49-52
Author(s):  
Elisângela De Souza Loureiro ◽  
Luis Gustavo Amorim Pessoa ◽  
Thayla Christiane Putrick ◽  
Ariane De Andréa Pantaleão ◽  
Pamella Mingotti Dias
Keyword(s):  
Pest Control ◽  
Oxygen Demand ◽  
Petri Dish ◽  
Colony Growth ◽  
Biological Pest Control ◽  
Colony Forming Units ◽  
In Vitro Inoculation ◽  
Petri Dishes ◽  
The One

Compatibility studies are essential for the integration and simultaneous use of chemical and biological pest control methods since they are necessary for an Integrated Pest Management (IPM) program. In this work, the aim was to evaluate the compatibility of insecticides used in soybean and cotton crops for pest control with Bacillus thuringiensis (Bt). The in vitro inoculation technique was used with B. thuringiensis var. kurstaki and B. thuringiensis var. aizawai, in culture medium containing the following insecticides: beta-cyfluthrin (Bulldock®), methomyl (Bazuka®), thiamethoxam + lambda-cialotrina (Engeo Pleno®), zeta-cypermethrin (Fury 200®), acetamiprid (Saurus®), bifenthrin + carbosulfano (Talisman®) and bifenthrin (Talstar®), in Petri dishes. The Petri dishes were taken to the B.O.D. (Biological Oxygen Demand), at a temperature of 30 ± 1 ºC, 70 ± 10% RH (relative humidity) and a photophase of 12 h, for 24 hours. Colony growth was measured, and Colony Forming Units (CFU) counted in the total area of the Petri dish. The product that allowed growth to be significantly equal to or higher than the control was established as compatible, and the one that did not allow growth or was significantly less than the control was incompatible. It was found that all insecticides were classified as incompatible with the bioinsecticide.

scholarly journals Characteristics of murine megakaryocytic colonies in vitro

Blood ◽  
1979 ◽  
Vol 54 (1) ◽  
pp. 169-179 ◽  
Author(s):  
SA Burstein ◽  
JW Adamson ◽  
D Thorning ◽  
LA Harker
Keyword(s):  
Conditioned Medium ◽  
Suicide Rate ◽  
Tritiated Thymidine ◽  
Colony Growth ◽  
Velocity Sedimentation ◽  
Agar Gel ◽  
Colony Forming Units ◽  
Granulocytic Colony

Abstract Characteristics of murine megakaryocytic colonies and their progenitor cells (CFU-m) were studied in vitro in agar gel. Colony growth required the presence of poke-weed-mitogen-stimulated spleen-conditioned medium. The number of colonies formed was linearly related to both the number of marrow cells plated and the amount of conditioned medium added. In addition, CFU-m were found in both the spleen and peripheral blood. Conditioned medium was also made without plasma, and this resulted in a cloning efficiency greater than that of conditioned medium prepared with plasma. The percentage of CFU-m in DNA synthesis was low (10%), as determined both in vivo and in vitro. Velocity sedimentation revealed that the majority of CFU-m sedimented at 4.3 mm/hr and had a tritiated thymidine (3H-TdR) suicide rate of 1.5 +/- 1.5%. A shoulder on the profile of CFU-m sedimented at approximately 6 mm/hr, with a suicide rate of 79 +/- 2%. Analysis of these data indicated that the majority of CFU-m were not in cycle or were in a long G1 period. The results suggest that CFU-m is a primitive progenitor, possibly closely related to murine splenic colony-forming units (CFU-s), analogous to erythroid bursts and granulocytic colony-forming units.

scholarly journals Chemical Composition and Antifungal Effects of Vitex agnus-castus L. and Myrtus communis L. Plants

2016 ◽  
Vol 44 (2) ◽  
pp. 466-471 ◽  
Author(s):  
Melih YILAR ◽  
Yusuf BAYAN ◽  
Abdurrahman ONARAN
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Plant Pathogens ◽  
Petri Dish ◽  
Myrtus Communis ◽  
Mycelium Growth ◽  
Agnus Castus ◽  
Petri Dishes

The purpose of this study was to assess the effectiveness of essential plant oils from Vitex agnus-castus L. (VAC) and Myrtus communis L. against the plant pathogens, Fusarium oxysporum f. sp. radicis-lycopersici (Sacc.) W.C. Synder & H.N. Hans, Rhizoctonia solani J.G. Kühn., Sclerotinia sclerotiorum (Lib.) de Bary and Verticillium dahliae Kleb., and to determine the chemical composition of the compounds in these essential oils. GC/MS analysis was identified 25 different compounds in VAC essential oil, while the main compounds were determined as Eucalyptol (17.75%), β-Caryophyllene (13.21%) and Spathulenol (10.41%). On the other hand, the essential oil of M. communis, consisted of 16 different compounds which were Eucalyptol (49.15%), Myrtenol (19.49%) and α-Pinene (8.38%) being its main compounds. An assessment of antifungal activity was performed under in vitro conditions. Plant pathogens were inoculated onto Petri dishes (60 mm) containing PDA medium (10 mL/Petri-1), and plant essential oils were applied at concentrations of 0.5, 1, 1.5, 2, 5 and 10 (μL/Petri-1) into the 5 mm diameter wells opened on the Petri dish surface. After that, the Petri dishes incubated at 22±2 °C. The results of this study, the essential oil of M. communis, at a dose of 10 μL/ Petri, inhibited the 100% mycelium growth of V. dahliae, S. sclerotiorum and R. solani. The highest dose of VAC essential oil was also 100% inhibited V. dahliae and S. sclerotiorum. The LC50 and LC90 values of M. communis and VAC essential oil calculated for V. dahliae, FORL, S. sclerotiorum and R. solani. This plant extracts were shown by in vitro conditions to be potential antifungal agents.

Sperm Performance Better on Diamond than on Polystyrene

2013 ◽  
Vol 1511 ◽  
Author(s):  
Andrei P. Sommer ◽  
Dan Zhu ◽  
Friedrich Gagsteiger ◽  
Hans-Jörg Fecht
Keyword(s):  
Embryonal Carcinoma ◽  
Petri Dish ◽  
Carcinoma Cells ◽  
Oxygen Species ◽  
Embryonal Carcinoma Cells ◽  
Vitro Fertilization ◽  
Polystyrene Petri Dish ◽  
Petri Dishes ◽  
New Generation

ABSTRACTRecently we postulated that polystyrene Petri dishes become soft when in contact with an aqueous milieu. Specifically, we assumed that the effect is restricted to a superficial nanolayer, a condition presumably favoring the establishment of a stable nanolayer of reactive oxygen species (ROS) at the liquid/solid-interface. Cells are known to be hypersensitive to ROS. Previously we used P19 mouse embryonal carcinoma cells and systematically analyzed their capability to climb different substrates placed vertically into a Petri dish. The worst and best performance was found on polystyrene (Petri dish material) and nanocrystalline diamond, respectively. Polystyrene Petri dishes are today standard in laboratories conducting in vitro fertilization (IVF). Here we proceed and extend the investigation to human spermatozoa and show that their performance (vitality) on polystyrene Petri dishes is low compared to that on diamond Petri dishes. This work may propel further research and inspire the development of a new generation of cell-friendly Petri dishes.

scholarly journals An Ultraviolet C-Band LED device for disinfection of air, articles, and indoor environment

2021 ◽  
Author(s):  
Anwesh Sahu
Keyword(s):  
Indoor Environment ◽  
Petri Dish ◽  
Constant Current ◽  
Practical Solution ◽  
Colony Forming Unit ◽  
Treat Ment ◽  
Colony Forming Units ◽  
Before And After ◽  
Ultraviolet C ◽  
Petri Dishes

<div> <div> <div> <p>Background: Commonly used items like wallets, keys, and phones are both tricky and impractical to disinfect from the dangers of harmful microbes. This study demonstrates the efficacy of UVC-LED technology in creating an efficient, useful, and practical solution.</p><p>Methods: As a demonstration of the efficacy of the UVC-LED light (275 nm), a panel of UVC LEDs was fabricated and was driven with a constant current electronic driver. Staphylococcus aureus and Escherichia coli were placed on Petri dishes, and placed 38 cm away from the UVC-LED panel. UVC flux measured at the petri dishes was 0.093 mW/cm2 . The method involved exposing both the bacteria to UVC treatment for 4 and 8 minutes. For each petri dish, the number of colony forming units were compared before and after the treatment and compared to the control.</p><p>Results: A significant reduction in colony forming unit (cfu) counts was found in all samples for both sets of bacteria: 97.9% in the 4 minutes treat- ment(22.3 mJ/cm2), and 99.9% in the 8 minutes treatment(44.6 mJ/cm2).</p><p>Conclusion: UVC-LED technology offers an effective, simple and inexpensive approach for disinfection. </p> </div> </div> </div>

scholarly journals Steroids and hematopoiesis. III. The response of granulocytic and erythroid colony-forming cells to steroids of different classes

Blood ◽  
1976 ◽  
Vol 48 (6) ◽  
pp. 855-864 ◽  
Author(s):  
JW Singer ◽  
JW Adamson
Keyword(s):  
Cell Cycle ◽  
Colony Growth ◽  
Target Cells ◽  
Naturally Occurring ◽  
Colony Forming Units ◽  
Granulocytic Colony ◽  
In Vitro Response ◽  
Stimulating Factor ◽  
Do So

Selected androgenic and nonandrogenic steroids enhance in vitro granulocytic and erythroid colony formation by mouse marrow cells, but do so by influencing either different target cells or cells in different states of cell cycle. Etiocholanolone, a naturally occurring nonandrogenic testosterone metabolite, permits cells not in active cycle to respond to colony-stimulating factor or erythropoietin. Fluoxymesterone, a synthetic androgen, appears to enhance colony growth by increasing the responsiveness of target cells to tropic stimuli. The majority of cells responding to this androgen are in active DNA synthesis. Direct comparison, however, of etiocholanolone-dependent erythroid or granulocytic colony-forming cells demonstrates nonidentity of the target cells. Thus colony-forming units responding to different classes of steroids are in different states of cell cycle and are physically separable. The enhancement of the in vitro response of colony-forming cells to regulating hormones by steroids such as etiocholanolane suggests a mechanism by which such agents may be therapeutically effective in certain cases of marrow failure in man.

The effects of ultra-high diluted gibberellic acid on in vitro lettuce seed germination.

2021 ◽  
Vol 15 (4) ◽  
pp. 50-54
Author(s):  
Carolina Santos Barreto ◽  
Fortune Homsani ◽  
Nina C Barboza Da Silva ◽  
Carla Holandino
Keyword(s):  
Gibberellic Acid ◽  
Lactuca Sativa ◽  
Germination Rate ◽  
Petri Dish ◽  
New Drugs ◽  
Lettuce Seed ◽  
Lettuce Seeds ◽  
Lactuca Sativa L ◽  
Petri Dishes

Lettuce seeds bioassays have been used in many different tests such as: alellopathyc models; developing of new drugs; ecotoxicity tests. In most cases, lettuce (Lactuca sativa L., Asteraceae) has been used because of its sensitivity, simultaneous and rapid germination, reliability of germination percentage and homogeneity of seeds. The main goal was to evaluate the effects of ultra-high diluted gibberellic acid (GA3) on lettuce seeds germination and seedling growth. Experiment was performed using Petri dishes containing one disk of Whatman nº01 paper watered with 1ml of water. In each Petri dish 10 lettuce seeds(Lactuca sativa L.) cv Regina 500 were placed and 2ml of the different treatment solutions were add: GA33µmol, GA3 3CH (10-6), GA3 12CH (10-24), water 12CH and water (no dilution and succussion). One milliliter solutions were added every 2 days of experiment. The experiment was repeated twice and each one consisted in 5 Petri dishes per treatment (n=100). All seeds were maintained in germination incubator under controlled temperature (25°C) and photoperiod (16L/8D). The tested substances were prepared according to Brazilian Homeopathic Pharmacopoeia (Brazil, 2011). The experiment was blinded all the time. All seeds germinated at same time (2 days) and after 7 days the germination rate was the same in all treatments. Root was affected just by Water 12 CH, in which shown the longest length (4.59 cm) when compared with others treatments. Shoot length was higher where gibberellin was added in concentration upper then Avogrado’s number.

scholarly journals Synergistic Antifungal Activity of Green Synthesized Silver Nanoparticles and Epoxiconazole against Setosphaeria turcica

2020 ◽  
Vol 2020 ◽  
pp. 1-7 ◽  
Author(s):  
Weidong Huang ◽  
Minhui Yan ◽  
Haiming Duan ◽  
Yaling Bi ◽  
Xinxin Cheng ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antimicrobial Agents ◽  
Integrated Control ◽  
Antimicrobial Effect ◽  
Colony Growth ◽  
Setosphaeria Turcica ◽  
Ligustrum Lucidum ◽  
In Vitro Inoculation ◽  
Average Size

It is urgent to develop highly efficient and eco-friendly antimicrobial agents for integrated control of phytopathogens. Silver nanoparticles (AgNPs) were synthesized by Ligustrum lucidum leaf extract. UV-vis spectrum showed that there was a strong absorbance at 438 nm. Transmission electron microscopy (TEM) images displayed that synthesized nanoparticles were near spherical with an average size of 13 nm. The antimicrobial effect of AgNPs was evaluated through methods of paper disk diffusion, colony growth, conidia germination, and in vitro inoculation. The 50% inhibition concentration (IC50) of AgNPs against Setosphaeria turcica was 170.20 μg/mL calculated by SPSS 13.0. In addition, it displayed a significant synergistic antifungal effect when AgNPs were combined with epoxiconazole at the ratios of 8 : 2 and 9 : 1. The results of this study provide a novel fungistat not only for comprehensive control of plant fungi but also for reducing chemical pesticides use and avoiding drug-resistant phytopathogen generation.

scholarly journals Characteristics of murine megakaryocytic colonies in vitro

Blood ◽  
1979 ◽  
Vol 54 (1) ◽  
pp. 169-179 ◽  
Author(s):  
SA Burstein ◽  
JW Adamson ◽  
D Thorning ◽  
LA Harker
Keyword(s):  
Conditioned Medium ◽  
Suicide Rate ◽  
Tritiated Thymidine ◽  
Colony Growth ◽  
Velocity Sedimentation ◽  
Agar Gel ◽  
Colony Forming Units ◽  
Granulocytic Colony

Characteristics of murine megakaryocytic colonies and their progenitor cells (CFU-m) were studied in vitro in agar gel. Colony growth required the presence of poke-weed-mitogen-stimulated spleen-conditioned medium. The number of colonies formed was linearly related to both the number of marrow cells plated and the amount of conditioned medium added. In addition, CFU-m were found in both the spleen and peripheral blood. Conditioned medium was also made without plasma, and this resulted in a cloning efficiency greater than that of conditioned medium prepared with plasma. The percentage of CFU-m in DNA synthesis was low (10%), as determined both in vivo and in vitro. Velocity sedimentation revealed that the majority of CFU-m sedimented at 4.3 mm/hr and had a tritiated thymidine (3H-TdR) suicide rate of 1.5 +/- 1.5%. A shoulder on the profile of CFU-m sedimented at approximately 6 mm/hr, with a suicide rate of 79 +/- 2%. Analysis of these data indicated that the majority of CFU-m were not in cycle or were in a long G1 period. The results suggest that CFU-m is a primitive progenitor, possibly closely related to murine splenic colony-forming units (CFU-s), analogous to erythroid bursts and granulocytic colony-forming units.

scholarly journals 804 PB 112 INFLUENCE OF CULTURE CONTAINER ON IN VITRO SHOOT PRODUCTION AND CALLUS FORMATION OF MUSCADINE GRAPE

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 548d-548
Author(s):  
Carol D. Robacker
Keyword(s):  
Culture Medium ◽  
Callus Formation ◽  
Petri Dish ◽  
Vitis Rotundifolia ◽  
Adenine Sulphate ◽  
Shoot Production ◽  
Clear Plastic ◽  
Petri Dishes ◽  
Muscadine Grape

Nodes from in vitro grown shoots of `Regale' and `Triumph muscadine grape (Vitis rotundifolia) were cultured in 25 × 95-mm glass vials, 25 × 150-mm glass culture tubes, 55 × 70-mm glass baby food jars and 100 × 25-mm plastic petri dishes. Culture medium consisted of Murashige and Skoog salts and vitamins, 80 mg/l adenine sulphate, 170 mg/l sodium phosphate, 2 mg/l BA and 8 g/l agar. Amount of medium dispensed was 8 ml per vial, 18 ml per culture tube and jar, and 70 ml per petri dish. Containers were covered with clear plastic lids, sealed with parafilm, and placed under fluorescent lights for six weeks. The number of shoots per explant that formed in petri dishes was three to four times greater than those formed on explants in vials, tubes and jars. However, the number of nodes per shoot were fewer in dishes than in the other containers. Callus formation was excessive in jars to the detriment of shoot production. Vials and tubes had small amounts of callus, while little or no callus was observed in dishes.

106 THE WARMING PROCEDURE: A FIRST STEP FOR IMPROVING THE NONSURGICAL DEEP INTRAUTERINE TRANSFER OF SOPS-VITRIFIED PORCINE EMBRYOS

2012 ◽  
Vol 24 (1) ◽  
pp. 165
Author(s):  
J. Gomis ◽  
C. Cuello ◽  
J. Sanchez-Osorio ◽  
M. A. Gil ◽  
I. Parrilla ◽  
...  
Keyword(s):  
Reproductive Performance ◽  
Petri Dish ◽  
Control Group ◽  
Hatching Rate ◽  
Embryo Viability ◽  
Step Method ◽  
One Step ◽  
The One

We previously reported successful nonsurgical deep intrauterine embryo transfer (ET) of fresh in vivo–derived porcine embryos. However, several trials from our laboratory demonstrated that when this procedure was used in combination with vitrified/warmed (VW) embryos, its efficiency was very low. Recently, we have shown that the one-step warming method in syringe, which was used in the earlier trials, compromises the in vitro embryo viability. The aim of this study was to confirm the negative effect of the direct warming in syringe and to evaluate the effect of alternative warming procedures on the in vivo development of VW embryos after nonsurgical ET. In Experiment 1, morulae and early blastocysts were collected on Days 5 to 6 (Day 0: onset of oestrus) and assigned to one of the following groups: 1) syringe group: vitrified embryos (n = 88) were warmed by the one-step method directly in a 1-mL syringe containing 300 μL of warming medium, which was connected to the ET catheter and then transferred to recipients (n = 6); 2) dish group: vitrified embryos (n = 194) were warmed with one-step warming method in a Petri dish containing 1 mL of warming medium, loaded into a Tom Cat catheter and transferred to recipients (n = 13); and 3) control group: fresh embryos (n = 129) were loaded in a 1-mL syringe in 100 μL of transfer medium and transferred to 9 recipients. An average of 15 embryos were transferred to each recipient on Day 4 or 5. Embryos were surgically recovered 24 h after ET. Data were analysed by ANOVA. The embryo recovery rate was similar among groups (range: 70.7 ± 4.8% to 77.2 ± 6.5%). The embryo survival (ES) and the hatching rate (HR) from the control group (94.0 ± 2.1% and 33.4 ± 7.6%, respectively) were higher (P ≤ 0.05) than those from the dish group (80.4 ± 4.6% and 14.5 ± 4.1%, respectively). All embryos from the syringe group were degenerated. Some viable recovered embryos (n = 135) were cultured for 48 h to evaluate their subsequent in vitro development. No differences were observed in ES between the control and the dish group (100.0 ± 0.0% vs 98.9 ± 1.0%). The HR in the control group (71.5 ± 2.1%) was higher (P ≤ 0.01) than that of the dish group (42.7 ± 6.1%). In experiment 2 we evaluated the reproductive performance of naturally cycling recipients after nonsurgical ET of vitrified embryos warmed with the one-step method in a Petri dish. An average of 35 VW morulae and blastocysts were transferred to each recipient (n = 10) on Days 4 to 6. Four recipients returned to oestrus at Days 21 to 22. The remaining 6 recipients were diagnosed pregnant by ultrasonography on Day 26. At Days 50 to 55, 5 recipients remained pregnant. In conclusion, the one-step in syringe warming method for vitrified porcine embryos had a completely adverse effect on the vivo viability, whereas nonsurgical ET of embryos warmed in a Petri dish allowed us to obtain promising reproductive performance. Supported by MICINN (AGL2009-12091) and SENECA (GERM 04543/07).

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