scholarly journals The research of antioxidant activity and its changes during storage of fruit and berry raw materials of the Sverdlovsk region

2019 ◽  
Vol 190 (11) ◽  
pp. 59-65
Author(s):  
Ольга Чугунова ◽  
Ol'ga Chugunova ◽  
Наталия Заворохина ◽  
Nataliya Zavorokhina ◽  
Антон Вяткин ◽  
...  

Abstract. Fruit and berry raw materials – a valuable source of the useful substances and antioxidants in a food allowance of the population of Sverdlovsk region. The purpose of researches was determination of the general antioxidant activity and its change at storage in the frozen type of fruit and berry raw materials. Material for a research were the fruit and berry raw materials growing in gardens of Sverdlovsk region including: sea buckthorn, raspberry, blackberry, blackcurrant, cherry, black-fruited chokeberry and shadberry. Method of research is inversion potentiometry. By results of the conducted researches it is established that the general antioxidant activity of a sea buckthorn was 2.204 mmol/l; ordinary raspberries – 1.976 mmol/l; gray blackberries – 0.513 mmol/l; blackcurrant – 8. 227 mmol/l; cherry ordinary – 4.971 mmol/l; black-fruited chokeberry – 8.026 mmol/l; shadberry roundleaf – 1.261 mmol/l. At storage of fruit and berry raw materials in a condition of freezing within 9 months of observations, made: at a sea-buckthorn to 0.416 mmol/l; at raspberry ordinary – to 0.225 mmol/l; at blackberry gray – to 0.113 mmol/l; at blackcurrant – to 4.173 mmol/l; at cherry ordinary – to 3.197 mmol/l; at an chokeberry black-fruited – to 2.754 mmol/l; at a shadberry roundleaf – to 0.229 mmol/l. It is proved that the growing in the gardens which are in the territory of Sverdlovsk region, fruit and berry raw materials can be an important source of antioxidants for consumers, and freezing allows to keep the useful antioxidant properties of berries for a period of up to 6 months.

2017 ◽  
Vol 63 (2) ◽  
pp. 34-41 ◽  
Author(s):  
Elżbieta Wojtowicz ◽  
Aldona Krupska ◽  
Renata Zawirska-Wojtasiak

Summary Introduction: Novel raw materials are being constantly searched for chicory coffee, which thanks to their specific composition can influence human health, thus promoting properties and of course the attractive aroma. Prior to their addition herbs – sea buckthorn (Hippophaë rhamnoides L.), rowanberry (Sorbus aucuparia L.), lovage roots (Levisticum officinale Koch) and dandelion (Taraxacum officinale coll.) – are roasted, which may change their antioxidant properties and generate free radicals with pro-oxidative properties. Objective: The characteristic of antioxidant activity of roasted herbal raw materials (sea buckthorn fruits, rowanberry, lovage roots and dandelion roots) for the production of chicory coffee and to determine the level of free radicals formed under the influence of roasting. Methods: Total phenolic contents were determined before and after the roasting process using the Folin-Ciocalteu phenol reagent and antioxidant activity by ABTS and DPPH assays. Free radicals were examined using electron paramagnetic resonance (EPR) spectroscopy. Results: The roasted materials were good sources of bioactive compounds, estimated at the total phenolic levels from 23.9 to 38.8 mg GAE/g. The highest antioxidant activity by ABTS and DPPH at 109.6 and 71.0 mg Tx/g was determined in roasted sea buckthorn. Free radicals were present in both the raw and roasted herbal materials. Roasted materials were characterised by markedly higher free radical concentrations when compared with the corresponding samples. The lowest free radical concentrations were recorded for roasted sea buckthorn fruits (0.031 × 1015 spin/g). Conclusion: Despite a high content of compounds with potent antioxidant activity, free radicals were present in novel roasted herbal materials. The formation of both antioxidant molecules and free radicals during the roasting process demonstrates that the two processes were not inversely related.


2021 ◽  
Vol 11 (18) ◽  
pp. 8685
Author(s):  
Elena Moroșan ◽  
Magdalena Mititelu ◽  
Doina Drăgănescu ◽  
Dumitru Lupuliasa ◽  
Emma Adriana Ozon ◽  
...  

Given the important role of antioxidants in the cellular degeneration process, as well as the increased interest in recent years related to the use of natural antioxidants in therapy, the present study aims to investigate the antioxidant activity of a new pharmaceutical product containing natural antioxidants extracted from plant raw materials. In a first step, the product conventionally named “CILTAG”, containing a mixture in equal proportions of 10% hydroalcoholic extractive solutions of dried plant raw materials from Medicaginis herba, Trifolii pratense flores, Ginkgo bilobae folium, Myrtilli fructus, and Cynosbati fructus, was obtained. In the second stage, the antioxidant activity of the hydroalcoholic extractive solutions included in the pharmaceutical product was tested by chemiluminescence and electrochemical methods and by the superoxide dismutase (SOD) method. The electrochemical determination of the antioxidant capacity of hydroalcoholic extractive solutions was based on a method that provides an indirect evaluation of the presumed antioxidant properties of some compounds using Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid), as a standard measurement. The experimental results indicate a significant antioxidant potential for both the analyzed plant extractive solutions that are part of CILTAG and the final product.


2019 ◽  
Vol 8 (4) ◽  
pp. 48-52
Author(s):  
O. V. Trineeva

Introduction. Recently, much attention has been paid to the primary assessment of the pharmacological effect of various drugs using in vivo and in vitro tests. It is known that such a medicinal plant as sea buckthorn, in its phytochemical composition is rich in natural antioxidants: carotenoids, tocopherols, flavonoids, ascorbic acid, etc. In some publications there is information about the antioxidant activity of sea buckthorn and fatty oil based on them. However, information on the comparative characteristics of the use of various methods for determining the antioxidant activity of this type of medicinal plant material and the results obtained are not found in the scientific literature.Aim. The aim of this work was a comparative determination of the antioxidant activity of medicinal plant material of buckthorn fruits of various species of buckthorn.Materials and methods. The total antioxidant activity of water and water-alcohol extracts from the fruits of sea buckthorn fruits was determined using various techniques recommended in the literature. The antioxidant activity of the extracts was determined by permanganometric titration, in vitro inhibition of adrenaline autooxidation, and also in a biological model, Parametium caudatum cell culture.Results and discussion. The effect of the extractant polarity on the value of antioxidant activity was studied. It was found that the highest content of antioxidants in the extraction is observed when using 96 % ethanol as an extractant.Conclusion. Using three methods, the prospects of using sea buckthorn fruits and preparations based on them as a source of antioxidants are shown. 


2014 ◽  
Vol 18 (07) ◽  
pp. 585-593 ◽  
Author(s):  
Sergey M. Kuzmin ◽  
Svetlana A. Chulovskaya ◽  
Vladimir I. Parfenyuk

Free radicals and reactive oxygen species (ROS) are important intermediate products in physiological processes. In the healthy cell, they are generated and regulated by enzymes and low molecular weight antioxidants. Overproduction of ROS leads to a large list of diseases, so the determination of antioxidant activity of perspective natural and synthetic compounds is necessary for drugs development. In this paper cyclic voltammetry (CV) method was applied to the electrochemical and superoxide scavenging properties of 5,10,15,20-tetrakis(4′-aminophenyl)porphyrin ( H 2 T (p- NH 2 Ph ) P ) and 5,10,15,20-tetrakis(3′-aminophenyl)porphyrin ( H 2 T (m- NH 2 Ph ) P ) investigation. It is shown that the studied porphyrins have very similar electrochemical behavior and slight effect on the quantity of superoxide produced during first CV cycle therefore the estimation of superoxide scavenging properties may be performed by the [Formula: see text] oxidation peak current monitoring. The antioxidant properties of porphyrins were estimated in terms of binding constants. The strong effect of NH 2 group position on the superoxide scavenging activity are shown: the H 2 T (p- NH 2 Ph ) P (kb = 12.7 × 102 mol-1) demonstrates the significant superoxide scavenging activities whereas H 2 T (m- NH 2 Ph ) P (kb = 0.83 × 102 mol-1) — negligible. The most probable mechanism of superoxide scavenges explaining the observed differences are the H -atoms transfer due to N – H bond breaking in the aminophenyl substituent.


Pharmacia ◽  
2020 ◽  
Vol 67 (2) ◽  
pp. 49-54
Author(s):  
Krassimira Yoncheva ◽  
Nadia Hristova-Avakumova ◽  
Vera Hadjimitova ◽  
Trayko Traykov ◽  
Petar Petrov

The study was focused on the evaluation of two copolymers as micellar carriers for kaempferol delivery. The copolymers comprised identical hydrophilic blocks of poly(2-(dimethylamino)ethyl methacrylate and different hydrophobic blocks of either poly(ε-caprolactone) (PDMAEMA9-b-PCL70-b-PDMAEMA9) or poly(propylene oxide) (PDMAEMA13-b-PPO69-b-PDMAEMA13). The calculation of Flory-Huggins parameters and determination of encapsulation efficiency showed that PDMAEMA-b-PCL-b-PDMAEMA copolymer possessed higher capacity for kaempferol loading. The diameter of the micelles before and after lyophilization was not changed, suggesting that the micelles could be lyophilized and redispersed before administration. The in vitro release of kaempferol from PDMAEMA-b-PPO-b-PDMAEMA micelles was faster than the release from PDMAEMA-b-PCL-b-PDMAEMA micelles, probably due to the higher affinity of kaempferol to this copolymer. Further, the higher affinity resulted in a retention of antioxidant activity of kaempferol in the presence of DPPH and KO2 radicals. Thus, PDMAEMA-PCL-PDMAEMA was considered more appropriate carrier because of the higher encapsulation efficiency and preservation of antioxidant activity of the drug.


Author(s):  
N. B. Eremeeva ◽  
N. V. Makarova

Plant extracts rich in polyphenols can be used in the food industry as natural preservatives, extending the shelf life of prepared and semi-finished foods without chemical preservatives. In this paper, we investigate the polyphenolic composition, antioxidant activity and antibacterial properties of herbal extracts as part of food systems. The research objects were knot grass (Polýgonum aviculáre), marjoram (Oríganum), bur beggar-ticks (Bídenstripartíta), thyme (Thymus), whortleberry leaves (Vaccínium ida vítis), calendula (Calendula), sage (Salvia), chamomile flowers (Matricāriachamomīlla), eucalyptus (Eucalýptus) and bearberry (Arctostáphylosúva-úrsi). We determined the total content of phenolic compounds and flavonoids; the antioxidant activity by DPPH and FRAP methods; variations in the bacterial сontamination of animal raw materials over total bacterial count (TBC), coliform bacteria, yeast/fungi, salmonella and staphylococcus. Extracts of sage (1138±57 mg GA/100 g and 537±25 mg C/100 g), eucalyptus (1073±49 mg GA/100 g and 412±20 mg C/100 g), chamomile flowers (1002±36 mg GA/100 g and 493±22 mg C/100 g) and marjoram (1015±42 mg GA/100 g and 458±21 mg C/100 g) contain the largest amount of biologically active substances (phenols and flavonoids, respectively). Sage, eucalyptus and chamomile extracts demonstrate the highest antioxidant activity among the studied samples. Most of the studied extracts exhibit little or no effect on the organoleptic properties of finished products. In addition, chamomile flower, sage and eucalyptus extracts suppress the growth of pathogenic microorganisms in foods under experimental conditions. The microflora growth is significantly reduced when treating animal raw materials with calendula flowers, marjoram and thyme extracts. Extracts of sage, chamomile flowers, calendula flowers, marjoram and thyme can be recommended as components of food raw materials.


2021 ◽  
pp. 165-173
Author(s):  
Ol'ga Leonidovna Saybel ◽  
Andrey Ivanovich Radimich ◽  
Grigoriy Vasil'yevich Adamov ◽  
Tamara Darizhapovna Dargaeva ◽  
Nikolay Borisovich Fadeev ◽  
...  

Chicory (Cichorium intybus L.) is a biennial or perennial herb of the Asteraceae family, growing in the Russian Federation under natural conditions in meadows, forest glades, grassy slopes, as well as on wastelands, fields, overgrown dumps and roadsides. Along with the wild-growing chicory, breeding varieties of cultivated plants are also known, intended for obtaining roots, while the aboveground part, which is a root rosette of leaves, is a production waste. The purpose of this study was to study the qualitative composition and antioxidant properties of biologically active substances (BAS) of the aerial part of cultivated chicory and to assess the potential for using secondary raw materials of this plant in the pharmaceutical industry for obtaining medicines. As a result of the study by HPLC-UV-MS / MS, it was found that the phenolic complex of the aerial part of the cultivated chicory is represented by phenol carboxylic acids - esters of caffeic, ferulic, coumaric acids with organic acids (quinic and tartaric) in various combinations; trace amounts of oxycoumarins (chicoriin); flavonoids (glycosides of quercetin, luteolin and isorhamnetin). The study of the antioxidant properties of BAS fractions of different polarity showed that the ethyl acetate fraction has the highest activity. It was found that the main dominant compound of this fraction is cichoric acid, which suggests that it is this metabolite that makes a decisive contribution to the antioxidant activity of the fraction and the extract as a whole.


2021 ◽  
pp. 388-392
Author(s):  
И.А. Громова ◽  
М.С. Воронина ◽  
Н.В. Макарова

Большое влияние на здоровье человека оказывают вещества, проявляющие антиоксидантный эффект, в значительной степени содержащиеся в ягодах малины и отходах сокового производства от данной ягоды, а именно свежезамороженной ягоде, свежевыжатом соке, водной вытяжке, сырых и высушенных выжимках и концентрированном соке. Целью работы стало изучение химического состава и антиоксидантной активности ягод малины и отходов сокового производства, наблюдение за изменением показателей исследуемых характеристик при механической и тепловой обработке ягод малины и выбор оптимального варианта. При проведении исследований были использованы следующие методы: определение общего содержания антоцианов; определение общего содержания фенольных соединений; определение общего содержания флавоноидов; определение антирадикальной активности; определение восстанавливающей силы; исследование антиоксидантных свойств с использованием модельной системы линолевой кислоты. Все методы по определению содержания антиоксидантов проводились на этанольных экстрактах. Установлено, что использование экстракции для ягод и отходов сокового производства позволяет более точно определить содержание исследуемых показателей. Выявлено, что наибольшая антиоксидантная активность 94,5-98,4 % отмечена в малине и водной вытяжке из нее. A great influence on human health is exerted by substances exhibiting an antioxidant effect, largely contained in raspberries and their juice waste products, specifically in fresh-frozen berries, fresh squeezed juice, water extract, raw and dried pomace and concentrated juice. The aim of the work was to study chemical composition and antioxidant activity of raspberries and their juice waste products, monitor changes in the parameters of the studied characteristics during mechanical and thermal processing of raspberries and select the optimal option. The following methods were used during the research: determination of the total content of anthocyanins; determination of the total content of phenolic compounds; determination of the total content of flavonoids; determination of antiradical activity; determination of regenerating power; study of antioxidant properties using a model system of linoleic acid. All methods for determining the content of antioxidants were carried out on ethanol extracts. It was established that using of extraction for berries and juice waste products made it possible to determine more precisely the content of the studied indicators. It was also revealed that the highest antioxidant activity of 94.5% - 98.4% was observed in raspberries and their water extract.


2018 ◽  
Vol 67 ◽  
pp. 03039
Author(s):  
Muhamad Sahlan ◽  
Charion Gibreth Hannes ◽  
Yanni Sudiyani ◽  
Heri Hermansyah ◽  
Anondho Wijanarko

One of the potential co-product of the manufacture of bioethanol made from oil palm empty fruit bunches are yeasts, which have antioxidant activity. Source of antioxidants can be obtained from vitamins and enzymes. In the characterization study of the antioxidant properties of yeast extract refers to a method performed by Xiong, by varying solvent and extraction time using hot water and ethanol 25%. From the variation of time and solvent extraction will be tested with the GSH content of alloxan method, to obtain the maximum concentration. The results show the isolation of amino acids with a hot solvent, at 15 minutes is better. These results are then carried out the characterization of amino acids with LCMS methods. An amino acid which has antioxidant activity is methionine, using the solvent water and tested methods of abortion LCMS and 3402.91 ppm ethanol with LCMS method implies 4137.002 ppm. Characterization of amino acids can then be used as a source of raw materials economic cosmetics worth selling.


Author(s):  
Neri M.

AbstractThe reduction of cytochrome c was taken as an index of the antioxidant activity of the whole smoke and the vapour phase. The method developed consists in the determination of the antioxidant activity in smoke by spectrophotometric and potentiometric determinations. Cigarette smoke was placed in direct contact with the buffer solution of the cytochrome c, thus avoiding the collection and extraction necessitated by other systems. The antioxidant activity of smoke was determined by spectrophotometric analysis as a function of thee variation in the absorption value of 550 nm caused by the reduced cytochrome, while the potentiometric analysis determines the rapid decrease in the reduction potential. The data obtained by these two methods corresponded perfectly with each other and they also showed that the above methods were reliable as an index of the antioxidant activity of smoke. Reduction of cytochrome in the smoke of ventilated cigarettes shows a difference in the vapour phase and not in the whole smoke.


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