scholarly journals Optimization of reproduction of ricefish endemic to Southeast Sulawesi Oryzias woworae Parenti & Hadiaty, 2010 through different sex ratios in spawning

2021 ◽  
Vol 21 (3) ◽  
pp. 235-251
Author(s):  
Mohamad Ayip Firmansyah ◽  
Mustahal Mustahal ◽  
Mas Bayu Syamsunarno ◽  
Muh Herjayanto

Oryzias woworae has a beautiful color and has been traded as ornamental fish. This species is an endemic ricefish from Muna Island, Southeast Sulawesi, and is threatened with endangered status. Information on the optimal spawning sex ratio in O. woworae is unknown. This study aimed was to examine the optimization of reproduction based on the sex ratio of male: female broodstock O. woworae involved in spawning. The sex ratio of male: female spawning broodstock used were 1:1, 1:2, 1:3, and 1:4. The male and female brooders used had a total length of 3,1 ± 0,5 cm and 2,5 ± 0,5 cm. After adaptation, the broodstock of O. woworae was put into aquariums according to the treatment, and each container contained three spawning substrates. The harvesting of eggs on the substrate is carried out two times a day. The results showed that spawning O. woworae with a ratio of 1♂: 4♀ produced the lowest number of eggs and was significantly different with ratios of 1♂: 1♀ and 1♂: 2♀ (P<0,05). The ratio of 1♂ : 3♀ gave the highest egg hatching of 55% but was not significantly different from other treatments (P>0,05). The difference in male and female ratios did not affect the survival rate of O. woworae larvae (P>0,05), with values from 91,9-100%. The highest larvae produced was found in the spawning ratio of 1♂ : 3♀ with 37 larvae but not significantly different from other treatments (P>0,05). The water quality values during the study were temperature 26,5-310C, pH 5,5-8,8, and dissolved oxygen 5,3-6,0 mg L-1. O. woworae broodstock spawning can be optimized with a male to female ratio of 1:3.

2015 ◽  
Vol 11 (2) ◽  
pp. 99-110 ◽  
Author(s):  
László Nowinszky ◽  
János Puskás

AbstractWe analysed the sex ratio of 32 macrolepidopteran species caught by Hungarian forestry light traps. That the ratio of males and females collected by light trap varies by species has been known for decades; however, the sex ratio found in the natural population is not known. All 32 species were processed separately, but by the same method. Both males and females were counted throughout the whole swarming. We calculated these figures and inspected the difference in the level of significance with the χ2 test. For each swarming we calculated the percentage of males and females. We also calculated the values of the variation coefficients, which express the deviations in average percentages.Males make up the majority of the moths captured in the trap; this result was mirrored by 29 of the 32 species investigated. One of the exceptions was the Pelosia muscerda Hfn. where we observed a male to female ratio that was equal. In addition to that Watsoniana cultraria Fabr. is the only one species captured by light traps that showed a significant female majority.Our results confirm that the majority of moths captured in traps are males. However, the proportion of males and females of each species, and even within the same species, tended to differ greatly with each swarming. Yet, it must be noted that these results speak only for those specimens captured by light traps and cannot be related directly to the actual sex ratio of populations living in the natural environment


Author(s):  
CC Nwafor ◽  
K Obioha ◽  
TO Akhiwu

Ascites is a symptom that can originate due to diverse pathologies. A lot of investigations including ascitic fluid cytology (AFC) can be done on it to help determine its origin. The aim of this study, is to document the findings and highlight the importance of AFC in patient care in Uyo. All AFC reports and slides in the Department of Histopathology, University of Uyo were retrieved, reviewed and used for this study. The age ranged from 1.5 – 80 years with mean age, 41.79 (±17.23) years. About 71.8% of the ascitic fluid (AF) specimens were from patients between the 3rd and 6th decade. Females predominated in all age groups expect 10-19 year's group, with a male to female sex ratio of 1:2.4. Malignant cells were seen in 28.7% of all the samples, while 51.2% were negative for malignant cells. Malignant cells were seen in 4 (6.7%), 11 (18.3%) and 6 (10%) of the AFC performed due to various liver pathologies, ovarian malignancies and intra-abdominal malignancies respectively. Malignant cells were found more in females with a male to female ratio of 1: 3.6. Age group 40-49 years accounted for most of the malignant cases (26.6%). The pattern of AFC in Uyo is similar to the pattern in other parts of Nigeria


2019 ◽  
Vol 147 ◽  
Author(s):  
Yuxue Liao ◽  
Yaqing He ◽  
Yan Lu ◽  
Hong Yang ◽  
Yanhua Su ◽  
...  

Abstract Hand, foot and mouth disease (HFMD) has spread widely and leads to high disease burden in many countries. However, relative transmissibility from male to female individuals remains unclear. HFMD surveillance database was built in Shenzhen City from 2013 to 2017. An intersex transmission susceptible–infectious–recovered model was developed to calculate the transmission relative rate among male individuals, among female individuals, from male to female and from female to male. Two indicators, ratio of transmission relative rate (R β ) and relative transmissibility index (RTI), were developed to assess the relative transmissibility of male vs. female. During the study period, 270 347 HFMD cases were reported in the city, among which 16 were death cases with a fatality of 0.0059%. Reported incidence of total cases, male cases and female cases was 0.0057 (range: 0.0036–0.0058), 0.0052 (range: 0.0032–0.0053) and 0.0044 (range: 0.0026–0.0047), respectively. The difference was statistically significant between male and female (t = 3.046, P = 0.002). R β of male vs. female, female vs. female, from female to male vs. female and from male to female vs. female was 7.69, 1.00, 1.74 and 7.13, respectively. RTI of male vs. female, female vs. female, from female to male vs. female and from male to female vs. female was 3.08, 1.00, 1.88 and 1.43, respectively. Transmissibility of HFMD is different between male and female individuals. Male cases seem to be more transmissible than female.


2017 ◽  
Vol 2 (4) ◽  
Author(s):  
Moulizar Moulizar ◽  
Aman Yaman ◽  
Muhammad Daud

Abstrak. Turunan hasil persilangan antara ayam lokal, ayam ras petelur dan ayam arab dihasilkan Ayam KAMARAS (Kampung - Arab – Ras).   Penelitian ini bertujuan untuk mengetahui pengaruh konsentrasi sperma terhadap fertilitas, daya tetas, bobot tetas DOC ayam KAMARAS dan Sex rasio. Konsentrasi sperma  ini berguna untuk mengetahui perbandingan jantan dan betina yang paling efisien dalam suatu pemeliharaan. Sperma ditampung dan dicampur dari 3 ekor ayam KAMARAS jantan yang berumur sekitar 7 bulan. Sperma yang telah diketahui konsentrasinya kemudian dibagi menjadi 4 bagian dan masing-masing diencerkan dengan NaCl fisiologis sehingga diperoleh konsentrasi akhir yaitu 25x106/0,5 ml (dosis 1); 50x106/0,5 ml (dosis 2); dan 75x106/0,5 ml (dosis 3) dan 100x106/0,5 ml (dosis 4). 24 ekor ayam KAMARAS betina diinseminasi untuk mengetahui pengaruh konsentrasi sperma terhadap daya tetas dan bobot tetas ayam KAMARAS. Ayam betina yang digunakan adalah ayam KAMARAS yang berumur sekitar 7 bulan.Hasil penelitian menunjukkan bahwa perlakuan konsentrasi sperma tidak berpengaruh nyata terhadap fertilitas telur namun disetiap perlakuan terdapat hasil yang berbeda-beda pada perlakuan P0: 25x106/0,5 ml tingkat fertilitas (100 %), P1: 50x106/0,5 ml (100%), P2: 75x106/0,5 ml dan pada perlakuan P3 terendah 83,33% diantara 4 perlakuan konsentrasi sperma yang terbaik untuk menghasilkan fertilitas  yang tinggi yaitu konsentrasi 50x106/0,5 ml. Pengaruh konsentrasi sperma terhadap daya tetas tidak berpengaruh nyata namun disetiap perlakuan terdapat hasil yang berbeda-beda pada perlakuan P0: 25x106/0,5 ml tingkat fertilitas (100 %), P1: 50x106/0,5 ml (100%), P2 75x106/0,5 ml (100%) dan pada perlakuan P3 terendah (83,33%) dari setiap perlakuan konsentrasi sperma yang dapat meningkatkan daya tetas telur adalah pada konsentrasi 50x106/0,5 ml dengan tingkat daya tetas (100%). Pengaruh konsentrasi sperma terhadap bobot tetas DOC KAMARAS berpengaruh nyata terhadap bobot tetas DOC pada setiap perlakuan P0 25x106/0,5 (31,1 g/butir), P1: 50x106/0,5 ml (33 g/butir), P2: 75x106/0,5 ml (32,1 g/butir) dan pada perlakuan P3 100x106/0,5 ml bobot tetas DOC sebesar (33,3 g/butir),  pengaruh konsentrasi sperma terhadap sex rasio didapat hasil pada perlakuan 50x106/0,5 ml-75x106/0,5 ml menghasilkan sex rasio DOC betina lebih dominan (66,6-75%).Effect of Sperm Concentration on Hatching Power and Hatching Weight of DAM Chicken KAMARASAbstract. Derivative results of crosses between local chickens, laying chicken and arab chicken produced Chicken KAMARAS (Kampung - Arab - Ras). This study aims to determine the effect of sperm concentration on fertility, hatchability, weight of DOC chicken KAMARAS and Sex ratio. This sperm concentration is useful for knowing the most efficient male and female ratio in a maintenance. Sperm was collected and mixed from 3 chickens KAMARAS male about 7 months old. The known sperm concentration is then divided into 4 parts and each diluted with physiological NaCl to obtain the final concentration of 25x106 / 0.5 ml (dose 1); 50x106 / 0.5 ml (dose 2); and 75x106 / 0.5 ml (dose 3) and 100x106 / 0,5 ml (dose 4). 24 chickens KAMARAS females inseminated to determine the effect of sperm concentration on hatchability and weight of hens chicken KAMARAS. The female chicken used is KAMARAS chicken which is about 7 months old.The results showed that sperm concentration treatment did not have significant effect on egg fertility but in each treatment there were different results on treatment of P0: 25x106 / 0,5 ml fertility rate (100%), P1: 50x106 / 0,5 ml (100% ), P2: 75x106 / 0.5 ml and at the lowest P3 treatment 83.33% among the 4 best sperm concentration treatment to produce high fertility ie concentration 50x106 / 0,5 ml. The effect of sperm concentration on hatchability was not significant but in each treatment there were different results on treatment of P0: 25x106 / 0,5 ml fertility rate (100%), P1: 50x106 / 0,5 ml (100%), P2 75x106 / 0.5 ml (100%) and at the lowest P3 treatment (83.33%) of each treatment of sperm concentration which can increase the hatchability of eggs at concentration 50x106 / 0,5 ml with hatchability level (100%). The influence of sperm concentration on DOC KAMARAS hatching weight significantly influenced the weight of DOC in each treatment P0 25x106 / 0,5 (31,1 g / grain), P1: 50x106 / 0,5 ml (33 g / grain), P2: 75x106 / 0,5 ml (32,1 g / grain) and at treatment of P3 100x106/ 0,5 ml of DOC hight weight (33,3 g / grain), influence of sperm concentration to sex ratio obtained result at treatment 50x106 / 0, 5 ml-75x106 / 0.5 ml result in sex ratio of female DOC is more dominant (66,6-75%).


1993 ◽  
Vol 71 (7) ◽  
pp. 1367-1371 ◽  
Author(s):  
Petr E. Komers ◽  
François Messier ◽  
Cormack C. Gates

Sexual segregation in ungulates has been documented for many species including bison. However, male and female bison do not differ in their pattern of habitat selection. In the present study we observed that a fraction of bison groups did not have young (<2 years) despite the presence of males and females. The male to female ratio in these groups was 2.4. We call them adult groups, in contrast to mixed groups, where young were present and the male to female ratio was 0.4. The proportion of cows with young (mothers) in a group was negatively correlated with the male to female ratio, suggesting that mothers associated more often with other cows than with bulls. Before the rut, cows without young (non-mothers) spent less time feeding than either mothers or bulls. However, mothers and non-mothers did not differ in the number of steps per minute they took while grazing, and both types of cows stepped faster than bulls. We suggest that cows feed more selectively than bulls and that the differing foraging strategies result in temporal but not spatial segregation of the sexes. Possibly as a result of similar nutritional demands, mothers tended to aggregate, forming nursery groups. We suggest that the formation of nursery groups can also serve to protect calves through a dilution effect of predation. Whether mothers actually prefer to associate with other mothers remains to be investigated.


2021 ◽  
Vol 15 (8) ◽  
pp. 2145-2147
Author(s):  
Lubna Riaz ◽  
Syed Muhammad Ahmed ◽  
Muhammad Ahmad Zakki ◽  
Neelam Faryad ◽  
Shazia Iram ◽  
...  

Background: Poor breathing effort results in decrease oxygen supply to brain and other organs that lead to birth asphyxia. Phenobarbital and magnesium sulphate are both neuroprotective to asphyxia! injury to brain. Objective: To compare the frequency of neonatal mortality with phenobarbital versus magnesium sulphate in the management of birth asphyxia Study Design: Randomized control trial Place and Duration of Study: Pediatrics Department, Sheikh Zayed Hospital, from 8th March 2020 to 8th September 2020. Methodology: One hundred and two neonates were enrolled. After taking informed consent from parents their demographic data was obtained. Then patients were divided in to two groups; group A treated with Phenobarbital and other group B treated with magnesium sulphate. Results: The mean age of group A neonates was 54.37+14.303 days and in group B 48.40+15.20 days with male to female ratio was 0.7:1. Adverse outcome occurred in 12 (11.54%) patients. Statistically insignificant difference (P=0.122) was found between groups. Conclusion: There is more adverse effects outcome with magnesium sulphate than phenobarbital however the difference was statistically insignificant for management of neonates with birth asphyxia. Keywords: Birth asphyxia, Neonates, Magnesium sulphate (MgS04), Phenobarbital


Blood ◽  
2012 ◽  
Vol 120 (21) ◽  
pp. 4979-4979
Author(s):  
Wu Yu ◽  
Huangling Zhu ◽  
Li Xiang Long ◽  
Ting Liu

Abstract Abstract 4979 Recently the essential role of macrophages in the drug resistance or pathogenesis of plasma cell myeloma has gradually been identified and investigated. Here we analyzed patients diagnosed as multiple myeloma in West China Hospital, Si Chuan University, China in the past 3 years. The study involved 156 patients, aged 34–81 year old, mean age 60. 4 year old, male to female ratio is 1. 47:1, including 2 and 3 stages of myeloma according to International Staging System. The therapeutic response was evaluated on the basis of criteria of International Myeloma Working Group Uniform Response Criteria in patients who had completed 3 therapeutic dexamethasone- containing cycles. We observed the involvement of macrophages in bone marrow by immunohistochemical staining of anti-CD68 monoclonal antibody (DAKO). During the observation, two pathologists without any knowledge of clinical data find the “hot spot ” of myeloma cells and then enumerated the number of macrophage in “ hot spots ” under 400 magnification. Interestingly, patients with more macrophage involvement (>40/hp) in bone marrow showed poorer response (including PR, VGPR and CR after 3 cycles of chemotherapy) (31. 2%) to Dexamethasone-containing chemotherapy and higher incidence of disease progression-related deaths. On the contrary, the patients with lower macrophage involvement demonstrated much better response (91. 6%) to chemotherapy and persistent remission (figure 1 the response rate analyzed by macrophage involvement). In summary, the difference of macrophage involvement in bone marrow of multiple myeloma patients with distinct prognosis suggested the macrophage inside bone marrow play a role in myeloma pathogenesis and therapeutic response. Disclosures: No relevant conflicts of interest to declare.


2020 ◽  
Vol 2020 (1) ◽  
pp. 225-233 ◽  
Author(s):  
Ralph Catalano ◽  
Joan A Casey ◽  
Tim A Bruckner

Abstract Background and objectives The sex ratio of human birth cohorts predicts the health and longevity of their members. Most literature invokes natural selection in support of the argument that heritable tendencies to produce male or female offspring induce oscillation in the sex ratio and its sequelae. Tests of the argument remain exceedingly rare because they require vital statistics describing many generations of a population both unaffected by migration and exposed to an exogenous stressor virulent enough to change the sex ratio at birth. We contribute to the literature by using time-series modeling to detect oscillation in the best data currently available for such a test. Methodology We apply rigorous time-series methods to data describing Sweden from 1751 through 1830, a period when the population not only aged in place without migration, but also exhibited the effects of an Icelandic volcanic eruption including a historically low secondary sex ratio. That very low sex ratio should have induced oscillation if heritable mechanisms appear in humans. Results We detected oscillation in the ratio but not that predicted by heritable tendencies to produce males or females. We found peak-to-trough oscillation at 14 rather than the approximately 32 years expected from the heritable tendencies argument. Conclusions and implications Our findings suggest that mechanisms other than perturbation of heritable tendencies to produce males or females induce oscillation in the human secondary sex ratio. These other mechanisms may include reproductive suppression and selection in utero. LAY SUMMARY The male to female ratio in human birth cohorts predicts longevity but its variation over time remains unexplained. We test the long-held theory that the ratio oscillates due to heritable tendencies to produce males or females. We find oscillation, but it appears due to social processes rather than heritable mechanisms.


2007 ◽  
Vol 10 (3) ◽  
pp. 508-513 ◽  
Author(s):  
Johan Fellman ◽  
Aldur W. Eriksson

AbstractIt is common opinion that the stillbirth rate is higher among monozygotic (MZ) than among dizygotic (DZ) twins. This is supported by the fact that stillbirth rates are higher among same-sexed than among opposite-sexed twins, and the relatively high stillbirth rates among twins of young mothers. In this study we present a method to estimate the stillbirth rates for MZ and DZ twins and identify the difference. We performed analyses based on the assumptions of (a) Weinberg's differential rule, including the assumption that the secondary sex ratio is 100, (b) the stillbirth rates among opposite-sexed twins hold for all DZ twins, and (c) the stillbirth rates estimated for MZ and DZ male and female twins yield for both sexes the observed total number of stillborn twins. Our methods are applied to data from Sweden, 1869–1967, the Åland Islands, 1750–1949, Saxony, 1881–1900, and England and Wales, 1996–2003. We observed that the ratio between the estimated stillbirth rates among MZ and DZ twins were on average 1.75, and the ratio among same-sexed and DZ (opposite-sexed) twins were on average 1.31. For Sweden and Saxony similar values were obtained, but for England and Wales the values were higher and for Åland lower. With exception of Åland, the estimated stillbirth rates were in all populations the lowest for DZ, medium for same-sexed and highest for MZ twins.


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