scholarly journals Effect of salicylic acid on some physiological parameters in micropropagated Bac Kan local alpinia (Alpinia coriandriodora D. Fang) under drought condition

Author(s):  
Bang Phi Cao ◽  
Duong Xuan Vu ◽  
Luong Trong Dang

In this work, some physiological parameters of micropropagated Bac Kan local alpinia (Alpinia coriandriodora D. Fang) affected by drought and salicylic acid treatments under drought condition were analyzed. In comparison to well watered plants, the droughted plants have lower contents of relative water, photosynthetic pigments, anthocyanins in leaves and Fv/Fm ratio. In constrat, proline content and activity of both catalase and peroxidase are higher in droughted plants. SA treatments, 10 M in particular, attenuate damages in droughted alpinia plants by affecting on some physiological parameters. An increase in content of relative water, photosynthetic pigments (chlorophylls and carotenoids), and anthocyanins were observed in leaves of treated plants in comparison to non-treated plants under drought condition. Fv/Fm ratio and peroxidase activity are higher in 10 M SA-treated plants than non-treated ones. Both 10 M and 100 M SA treatments lead to a decrease in proline accumulation and catalase activity in leaves of droughted plants.

2018 ◽  
Vol 2 (1) ◽  
pp. 42
Author(s):  
Adisa Paric ◽  
Erna Karalija

Drought-induced stress is the main limiting factor that affects growth and development in plants. In this study, the effect of exogenous application of salicylic acid (SA) on drought tolerance in basil was investigated. The analysis showed that application of SA under drought stress had significant effects on physiological and biochemical parameters, such as photosynthetic pigments content, total phenolics, flavonoids, flavanols and protein content and peroxidise activity, but had no significant effects on the morphological parameters, such as stem length, length and areaof leaves . In drought conditions, total phenolics and peroxidase activity reduced significantly, but all photosynthetic pigments, total flavonoids, flavanols and proteins increased significantly. Application of SA displayed some alleviating effects against drought induced stress through increase of plant growth, total flavonoids content and peroxidase activity


2017 ◽  
Vol 1 ◽  
pp. 249
Author(s):  
Anaz Nasiri ◽  
Morteza Samdaliri ◽  
Amirhossein Shirani Rad ◽  
Nasser Shahsavari ◽  
Amirabbas Mosavi Kale ◽  
...  

An experiment was performed in the Seed and Plant Improvement Institute, Karaj, Iran to study the influence of plant densities on physiological traits of six canola cultivars. Treatment conditions included three different plant densities (40, 60, and 80 plants m-2) for triplicates of six canola cultivars, namely, Ahamadi, Opera, Okapi, L72, Karaj1, and Sw102. Results indicated that L72 cultivar exhibited the highest yield at the lowest plant density (40 plants m-2). For all cultivars, both plant densities of 60 and 80 plants m-2 resulted in lower relative water content than 40 plants m-2. Proline and carbohydrate content significantly increased with increasing plant densities. The highest proline content was obtained from L72 under the highest plant density, whereas the lowest was also detected in this cultivar at the lowest plant density. All cultivars grown at the lowest density showed higher amounts of photosynthetic pigments chlorophylls a and b than those at the highest density. Glucosinolate increased with increasing plant densities, with L72 yielding the highest quantity when grown at the highest density. In conclusion, a density of 40 plants m-2 is recommended for growing L72 in this region. 


2017 ◽  
Vol 27 (2) ◽  
pp. 161-169 ◽  
Author(s):  
Lidiia Samarina ◽  
Valentina Malyarovskaya ◽  
Yulija Abilfazova ◽  
Natalia Platonova ◽  
Kristina Klemeshova ◽  
...  

Structural and physiological responses of chrysanthemum to repeated osmotic stress were studied. Plants were cultured for 2 weeks (for each stress1 and stress 2) on half MS supplemented with mannitol 100 mM (Treatment I) and 200 mM (Treatment II). First stress inhibited growth parameters stronger than second stress in treatment I. In treatment II both stress events strongly inhibited growth parameters of micro‐shoots. Proline content exceeded control 6 ‐ 8 times after 1st stress, and 2 ‐ 5 times after the 2nd stress in treatments I and II, respectively. Soluble protein was accumulated in leaves during both stress exposures, and 2 ‐ 2.5 times exceeded control after the 2nd stress. Relative water content in both treatments increased after the 2nd stress exposure. In treatment II chlorophyll а and carotenoids contents were 8.78 and 4.62 mg/g comparing to control (4.21 and 2.25 mg/g, respectively) after the 1st stress. But after the 2nd stress there was no difference with control.Plant Tissue Cult. & Biotech. 27(2): 161-169, 2017 (December)


Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1349
Author(s):  
Ahlam Khalofah ◽  
Mona Kilany ◽  
Hussein Migdadi

Heavy metals are primarily generated and deposited in the environment, causing phytotoxicity. This work evaluated fenugreek plants’ morpho-physiological and biochemical responses under mercury stress conditions toward Ag nanoparticles and Sphingobacterium ginsenosidiumtans applications. The fabrication of Ag nanoparticles by Thymus vulgaris was monitored and described by UV/Vis analysis, FTIR, and SEM. The effect of mercury on vegetative growth was determined by measuring the root and shoots length, the number and area of leaves, the relative water content, and the weight of the green and dried plants; appraisal of photosynthetic pigments, proline, hydrogen peroxide, and total phenols content were also performed. In addition, the manipulation of Ag nanoparticles, S. ginsenosidiumtans, and their combination were tested for mercury stress. Here, Ag nanoparticles were formed at 420 nm with a uniform cuboid form and size of 85 nm. Interestingly, the gradual suppression of vegetal growth and photosynthetic pigments by mercury, Ag nanoparticles, and S. ginsenosidiumtans were detected; however, carotenoids and anthocyanins were significantly increased. In addition, proline, hydrogen peroxide, and total phenols content were significantly increased because mercury and S. ginsenosidiumtans enhance this increase. Ag nanoparticles achieve higher levels by the combination. Thus, S. ginsenosidiumtans and Ag nanoparticles could have the plausible ability to relieve and combat mercury’s dangerous effects in fenugreek.


Water ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 1316
Author(s):  
Abida Parveen ◽  
Muhammad Arslan Ashraf ◽  
Iqbal Hussain ◽  
Shagufta Perveen ◽  
Rizwan Rasheed ◽  
...  

The present work reports the assessment of the effectiveness of a foliar-spray of salicylic acid (SA) on growth attributes, biochemical characteristics, antioxidant activities and osmolytes accumulation in wheat grown under control (100% field capacity) and water stressed (60% field capacity) conditions. The total available water (TAW), calculated for a rooting depth of 1.65 m was 8.45 inches and readily available water (RAW), considering a depletion factor of 0.55, was 4.65 inches. The water contents corresponding to 100 and 60% field capacity were 5.70 and 1.66 inches, respectively. For this purpose, seeds of two wheat cultivars (Fsd-2008 and S-24) were grown in pots subjected to water stress. Water stress at 60% field capacity markedly reduced the growth attributes, photosynthetic pigments, total soluble proteins (TSP) and total phenolic contents (TPC) compared with control. However, cv. Fsd-2008 was recorded as strongly drought-tolerant and performed better compared to cv. S-24, which was moderately drought tolerant. However, water stress enhanced the contents of malondialdehyde (MDA), hydrogen peroxide (H2O2) and membrane electrolyte leakage (EL) and modulated the activities of antioxidant enzymes (superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), as well as accumulation of ascorbic acid (AsA), proline (Pro) and glycine betaine (GB) contents. Foliar-spray with salicylic acid (SA; 0, 3 mM and 6 mM) effectively mitigated the adverse effects of water stress on both cultivars. SA application at 6 mM enhanced the shoot and root length, as well as their fresh and dry weights, and improved photosynthetic pigments. SA foliage application further enhanced the activities of antioxidant enzymes (SOD, POD, and CAT) and nonenzymatic antioxidants such as ascorbic acid and phenolics contents. However, foliar-spray of SA reduced MDA, H2O2 and membrane permeability in both cultivars under stress conditions. The results of the present study suggest that foliar-spray of salicylic acid was effective in increasing the tolerance of wheat plants under drought stress in terms of growth attributes, antioxidant defense mechanisms, accumulation of osmolytes, and by reducing membrane lipid peroxidation.


1996 ◽  
Vol 316 (1) ◽  
pp. 251-257 ◽  
Author(s):  
Michinori MUTSUDA ◽  
Takahiro ISHIKAWA ◽  
Toru TAKEDA ◽  
Shigeru SHIGEOKA

Synechococcus PCC 7942, a cyanobacterium, possesses catalase–peroxidase as the sole hydrogen peroxide-scavenging system. The enzyme has been purified to electrophoretic homogenenity from the cells. The native enzyme had a molecular mass of 150 kDa and was composed of two identical subunits of molecular mass 79 kDa. The apparent Km value of the catalase activity for H2O2 was 4.2±0.27 mM and the kcat value was 2.6×104 s-1. The enzyme contained high catalase activity and an appreciable peroxidase activity with o-dianisidine and pyrogallol. The catalase activity was not inhibited by 3-amino-1,2,4-triazole but by KCN and NaN3 (apparent Ki values 19.3±0.84 and 20.2±0.95 μM respectively). The enzyme showed an absorption spectrum of typical protohaem and contained one protohaem molecule per dimer. The gene encoding catalase–peroxidase was cloned from the chromosomal DNA of Synechococcus PCC 7942. A 2160 bp open reading frame (ORF), coding a catalase–peroxidase of 720 amino acid residues (approx. 79.9 kDa), was observed. The deduced amino acid sequence coincided with that of the N-terminus of the purified enzyme and showed a remarkable similarity to those of a family of catalase–peroxidases of prokaryotic cells. Escherichia coli BL21(DE3)plysS, harbouring a recombinant plasmid containing the catalase–peroxidase gene, produced a large amount of proteins that co-migrated on SDS/PAGE with the native enzyme. The recombinant enzyme showed the same ratio of catalase activity to peroxidase activity with o-dianisidine and the same Km for H2O2 as the native enzyme.


2021 ◽  
Vol 176 ◽  
pp. 111505
Author(s):  
Estevão Perin Gomes ◽  
Cristine Vanz Borges ◽  
Gean Charles Monteiro ◽  
Matheus Antonio Filiol Belin ◽  
Igor Otavio Minatel ◽  
...  

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