scholarly journals EVALUATION OF ANTIOXIDANT ACTIVITY OF CHLOROPHYLL

2019 ◽  
Vol 8 (6) ◽  
Author(s):  
Dipti Acharya ◽  
Umesh Kumar Gilhotra ◽  
Ranjan Singh ◽  
Himanshu Rajpurohit ◽  
Ajay Gar
Keyword(s):  
Oxidative Stress ◽  
Nitric Oxide ◽  
Hydrogen Peroxide ◽  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Antioxidant Properties ◽  
Antioxidant Potential ◽  
Methanolic Solution ◽  
Ic50 Value

Antioxidant play an important role to protect damage caused oxidative stress (OS).Chlorophyll having phenolic contents are reported to possess antioxidant properties. The present study was designed to investigate the antioxidant properties of methanolic solution from Super Chlorophyll. Objective: The main objective of the study was to evaluate the antioxidant activity of the chlorophyll in different validated in vitro models. Methods: The antioxidant activities of methanolic solution were evaluated by in vitro standard method using UV-Spectrophotometer. The antioxidant activity were determined bt total antioxidant capacity, DPPH (1-1-diphennyl-2-picrylhydrzine) radical scavenging assay, Nitric Oxide scavenging assay and Hydrogen Peroxide scavenging assay methods. Result: The solution of Chlorophyll was studied for antioxidant potential. Ascorbic acid was used as standard. This method is simple and activity of the solution is reported in term of IC50 value. Antioxidant properties depend on the IC50 value. Lesser the IC50 value, more is the antioxidant activity. Chlorophyll showed good antioxidant activity. Conclusion:  In case of in vitro antioxidant activity, Chlorophyll scavenged DPPH, hydrogen peroxide, nitric oxide radicals significantly, showed IC50 values near to standard ascorbic acid, thus proving to have good antioxidant potential. Keywords: Oxidative stress, DPPH, Antioxidant, Super chlorophyll.

scholarly journals Evaluation of the antioxidant activity of various extracts of aerial parts of Cassia absus: An in-vitro techniques

2020 ◽  
Vol 11 (2) ◽  
pp. 1545-1550
Author(s):  
Mythri M ◽  
Sanal Dev K T ◽  
Kottai Muthu A
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Antioxidant Potential ◽  
In Vitro Techniques ◽  
Aerial Parts ◽  
Ic50 Value ◽  
Total Antioxidant ◽  
The Difference

Cassia absus (Linn)Cassia absus(Linn) (family Fabaceae ) is generally known as “chaksu ” inan ayurvedic traditional system.The current study,aerial parts of different concentrates(Pet.ether, ethyl acetate and methanol) of Cassia absus, was evaluated for its in-vitro antioxidant potential byDiphenylpicrylhydrazyl radical,nitric oxide activity andtotal antioxidant activitytaking ascorbate as the standardfor all the three methods. The IC50 value was originated that methanolic concentrates of Cassia absusmore efficient inDiphenylpicrylhydrazyl radical,nitric oxide activity, total antioxidant activitycompared EA&PEconcentrates.The methanolic concentrates of Cassia absus& ascorbic acid exhibited antioxidant potential possessing IC50230µg/ml &130µg/ml (Nitric oxide). 205µg/ml &57µg/ml (total antioxidant),195µg/ml & 66µg/ml (Diphenylpicrylhydrazyl radical)respectively. The difference in the scavenging potential of the extracts can be due to variation in the percentage of bioactive compounds present in different solvents. Invitroantioxidant studiesobviouslyshow the methanolic concentrates of Cassia absushave better antioxidant activity. This result indicates that aerial parts of methanolic concentratesCassia absuscould serve as a natural antioxidant, which may be useful in preventfree radical-induced diseases.

scholarly journals In vitro antioxidant properties and inhibitory effect of extracts and fractions of Plectranthus glandulosus leaves on copper sulfate (CuSO4)-induced oxidation in human low-density lipoprotein

2020 ◽  
Vol 10 (4) ◽  
pp. 133-145
Author(s):  
Djamila Zouheira ◽  
Gabriel A Agbor ◽  
Randhir Singh ◽  
Sylviane Laure Poualeu Kamani ◽  
Anu Kajal ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Hydrogen Peroxide ◽  
Ascorbic Acid ◽  
Optical Density ◽  
Research Work ◽  
Antioxidant Properties ◽  
Antioxidant Potential ◽  
Ldl Oxidation ◽  
Scavenger Activity

Oxidation of LDL has been suggested to be an initial step in the development of atherosclerosis. This research work deals with the evaluation of antioxidant potential of Plectranthus glandulosus leaves extracts and fractions as well as its protective effects against human LDL oxidation. A preliminary quantitative phytochemical screening was carried out. Antioxidant potential was evaluated employing in vitro hydrogen peroxide, nitric oxide scavenging assays and TAOC test. The human LDL oxidation induced by CuSO4 inhibition test was also performed. Plectranthus glandulosus leaves exhibited the presence of 18.3% of saponines, 25.6% of terpenoids and 36.2% of flavonoids. EAF exhibited highest hydrogen peroxide and nitric oxide scavenger activity (IC50 13.63µg/ml and 24.59 µg/ml respectively). Ascorbic acid exhibited an IC50 value of 15.39µg/ml in hydrogen peroxide assay and 22.96 µg/ml in nitric oxide scavenging activity. EAF exhibited a higher TAOC (optical density 0.186±0.00) than that of ascorbic acid (0.162±0.001) at the concentration of 25µg/ml while at 200µg/ml N-BUTF exhibited a higher optical density (1.261±0.001) than that of ascorbic acid (1.065±0.001). EAF lengthened the lag time of the CD formation up to 150mins at the concentration 1mg/ml. TBARS formation inhibition at the concentration 0.5mg/ml, were no significant different between n-butanol (68.55%) ethyl acetate fraction (68.21%) and quercetin 67.69%). Plectranthus glandulosus is a promising prospect as an anti-atherosclerotic agent and needs a detailed study to establish the same. Key words: Plecthrantus glandulosus; oxidation; LDL; atherosclerosis, antioxidant.

scholarly journals Synthesis of some novel 3- (4-ethoxyphenyl) -5- (4-substituted) -4,5-dihydro-1H-pyrazole derivatives as potent antioxidant agents

2020 ◽  
Vol 11 (2) ◽  
pp. 1571-1577
Author(s):  
Lakshminarayanan B ◽  
Kannappan N ◽  
Subburaju T ◽  
Kalaichelvan V K
Keyword(s):  
Hydrogen Peroxide ◽  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Free Radical ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Assay Method ◽  
Antioxidant Agents ◽  
Ic50 Value

Pyrazolines are the most useful heterocyclic moiety in Pharmaceutical and Chemical fields and as the most potential molecules for the design of new chemical entities. Nitrogen-containing heterocyclic compounds, pyrazolines and their derivatives showed a variety of pharmacological activities, including antioxidant properties. In the present study, eleven novel ethoxylated pyrazoline derivatives were synthesized by condensing chalcones with electron releasingethoxy group at one end and different electron-donating, electron-withdrawing groups in another end with hydrazine hydrate andalcohol. The compounds synthesized were structural elucidated by their spectroscopic studies. All the compounds synthesized were evaluated for their in vitro antioxidant potential by 2,2’-diphenyl-1-picrylhydrazyl (DPPH)and hydrogen peroxide free radical scavenging assay methods.Some of these molecules possess moderate to good antioxidant activitywhen compared to standard ascorbic acid. The compound with methoxy group (EH2) exhibits potent antioxidant activity with IC50 value of 9.02 and 9.44µg/ml in DPPH and hydrogen peroxide assay method respectively and the compound with hydroxy group (EH9) also showed potent antioxidant activity with IC50 value of 12.41 and 14.56µg/ml in DPPH and hydrogen peroxide free radical scavengingassay method respectively when compared to standard. The compounds containing electron-donating substituents were found to be good antioxidantswhen compared to standard ascorbic acid.

scholarly journals Comparative Antioxidant Potential of Different Extracts of Celastrus paniculatus Willd. Seed

1970 ◽  
Vol 3 (1) ◽  
pp. 68-74 ◽  
Author(s):  
Fatema Tuz Zohera ◽  
Md Razibul Habib ◽  
Mohammad Zafar Imam ◽  
Md Ehsanul Hoque Mazumder ◽  
Md Sohel Rana
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Reducing Power ◽  
Antioxidant Potential ◽  
Dependent Manner ◽  
Ic50 Value ◽  
Celastrus Paniculatus

The objective of the present study was to evaluate the comparative antioxidant potential of methanol, ethyl acetate, pet ether and water extracts of Celastrus paniculatus seed. Antioxidant activity was evaluated by using total phenol and flavonoid content determination assays, total antioxidant capacity, 1,1-diphenyl-2- picryl-hydrazil (DPPH) free radical assay, Reducing power assessment, Nitric oxide (NO) scavenging assay and Cupric ion reducing capacity assay (CUPRAC method). The extracts showed moderate antioxidant activity in a dose dependent manner. The extracts were found to contain phenolics and flavonoid compounds. In DPPH radical scavenging assay, ethyl acetate extract had the lowest IC50 value (585.58μg/ml) compared to ascorbic acid. In nitric oxide scavenging assay IC50 value was found to be 122.99μg/ml, 320.54μg/ml, 601.81μg/ml and 206.37μg/ml respectively for the Water, Methanol, Ethyl Acetate and Pet Ether extracts compared to 6.83μg/ml which was the IC50 value for the reference ascorbic acid. The extracts also showed good reducing power. The results of the present study indicate that the extracts possesses significant antioxidant potential of which ethyl acetate extract is the most promising one and possess highest antioxidant potential. Key Words: Celastrus paniculatus; antioxidant; DPPH; NO scavenging; CUPRAC; ROS. DOI: 10.3329/sjps.v3i1.6802S. J. Pharm. Sci. 3(1): 68-74

scholarly journals In Vitro Antioxidant Properties of Gum Extract From The Carob (Ceratonia silique L.) Plant

2021 ◽  
Vol 34 (1) ◽  
pp. 84-93
Author(s):  
Marwah T. G. Al-Ameri ◽  
Amera K. Nasser
Keyword(s):  
Hydrogen Peroxide ◽  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Antioxidant Properties ◽  
Ferrous Ion ◽  
College Of Agriculture ◽  
Active Compounds ◽  
Significant Difference ◽  
Active Substances

The experiment was conducted in the laboratories of the College of Agriculture, University of Basra, for the period from 22/9/2019 to 25/ 11/2019. The current study aims to extract gum from the carob fruits, to determine the active substances and the possibility of using them as antioxidants. The results showed that the gum of the fruits of the carob plant contains all the active compounds under study, which included Alkaloids, Phenols, Flavonoids, Glycosides, Steroids, Tannins, Resins, Saponins and Coumarins. The results also showed that the gum of the fruits of the carob plant showed antioxidant activity of about 84.55 % when used at a concentration of 0.2 %, while the average antioxidant efficacy of the industrial compound Butylated Hydroxy Toluene (BHT) and Ascorbic acid was about 93.43 %, 91.12 % at the same concentration. The Reductive power were about 73.20 % and 83.30 % with a concentration of 0.1 % and 0.2 % respectively, which was comparable to the effect of industrial antioxidants (BHT) and ascorbic acid with a concentration of 0.2 %, which was 91.40 % and 90.70 %, respectively. In addition, the gum of the fruits of the carob plant has the potential to sweep hydrogen peroxide with a capacity similar to ascorbic acid and without significant difference (p≤0.05), reaching 41 % and 50 % for concentrations 0.1 % and 0.2 %, respectively, while ascorbic acid reached 45 % with the presence of moral differences with the industrial antioxidant (BHT) in its susceptibility to the sweep of hydrogen peroxide at 73 %. The results also showed that the gum of the fruits of the carob plant is able to bind to the Ferrous ion and this portability increased with the increased concentration of gum to reach its highest average of 43.19 % at the concentration of 0.2 % while the average Ferrous ion for antioxidant (EDTA 2Na) was 55.48 %.

scholarly journals Synthesis and Antioxidant Properties of Novel 2-(2,4-Dioxothiazolidin-5-ylidene)-Acetamides Containing 1,3,4-Thia/Oxadiazole Moieties

2021 ◽  
Vol 12 (5) ◽  
pp. 6710-6722
Keyword(s):  
Ascorbic Acid ◽  
Acetyl Chloride ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Scavenging Effect ◽  
Highly Active ◽  
Ethanol Medium ◽  
Ic50 Value ◽  
Radical Scavenging Ability

A series of novel 1,3,4-thia(oxa)diazole substituted 2-(2,4-dioxothiazolidine-5-ylidene)-acetamides 3a-c, 4 and 5a-k have been synthesized following the acylation reaction of 2-amino-5-aryl-1,3,4-oxadiazoles, 5-amino-1,3,4-thiadiazole-2-thiol and it’s S-alkylated derivatives with 2-(2,4-dioxothiazolidine-5-ylidene)acetyl chloride in dioxane medium. The functionalization of compounds 3b, 3c, 5d and 5e was carried out on their N3 position under N-alkylation conditions with N-aryl-2-chloroacetamides in DMF/ethanol medium yielded the corresponding 2,4-dioxothiazolidine-3,5-diacetic acid diamides 6a-e and 7a-b. The structures of target compounds were confirmed by using 1H NMR spectroscopy and elemental analysis. The antioxidant activity evaluation in vitro of the synthesized compounds was performed by the method of scavenging effect on 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals. As a result, the highly active compound 4, namely 2-(2,4-dioxothiazolidin-5-ylidene)-N-(5-mercapto-[1,3,4]thiadiazol-2-yl)acetamide was found to be the most efficient candidate among all compounds with a radical scavenging ability of 88.9%, which was comparable that for ascorbic acid (92.7%). The experimentally calculated IC50 value of 43.1 µM for compound 4 was lower than for ascorbic acid (50.5 µM).

In Vitro Study of Antioxidant Activity of Serum and Plasma Samples As Well As Glutathione Exposed to Various Exogenous Stress Factors

2021 ◽  
Author(s):  
Małgorzata Olszowy-Tomczyk ◽  
Łukasz Paprotny ◽  
Agnieszka Celejewska ◽  
Dorota Szewczak ◽  
Dorota Wianowska
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Antioxidant Properties ◽  
In Vitro Study ◽  
Stress Factors ◽  
Plasma Samples ◽  
Serum Samples ◽  
Exogenous Stress

Abstract The imbalance between the production of Reactive Oxygen Species (ROS) and their sequestration promotes the formation of so-called oxidative stress conditions which are considered crucial in the aging process and development of many human diseases. Glutathione plays an essential role in the antioxidative barricade against ROS. Its role in the detoxification process of xenobiotics and carcinogen is also known. However, there are no comparative studies on the antioxidant properties of both biological samples and glutathione as well as the change in these properties as a result of exposure to various stress factors. This paper fills this gap comparing the antioxidant activity of serum and plasma samples of the known glutathione content with the activity of glutathione itself assessed by the different methods. In addition, it reveals a significant role of environmental xenobiotics in oxidative stress and differentiates the stress induced by different groups of drugs, among which the greatest one has been demonstrated for antiarrhythmic drugs and cytostatics. More importantly, it proves that human plasma is more resistant to stress factors and N-acetylcysteine clearly promotes the extension of antioxidant properties of both the plasma and serum samples. The latter conclusion is consistent with the implied preventive and/or supportive action of this drug against SARS-CoV-2.

scholarly journals Amaranth Leaf Extract Protects Against Hydrogen Peroxide Induced Oxidative Stress in Drosophila Melanogaster

2021 ◽  
Author(s):  
Johnmark Ndinawe ◽  
Hellen W. Kinyi
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Drosophila Melanogaster ◽  
Leaf Extract ◽  
Dependent Manner ◽  
Polyphenol Compounds ◽  
Dose Dependent

Abstract ObjectiveAmaranths leaves are rich in ascorbic acid and polyphenol compounds which have antioxidant activity. The aim of this study was to evaluate their in vivo antioxidant activity. The effect of consumption of Amaranth leaf extract on in vivo antioxidant activity, catalase enzyme activity and H2O2 induced oxidative stress in Drosophila melanogaster flies was assessed.ResultsConsumption of Amaranth leaf extract was associated with increased survival on exposure to H202 in a dose dependent manner in Drosophila melanogaster flies.

scholarly journals Antioxidant Potential and Peroxidative State of `Golden Smoothee' Apples Treated with 1-Methylcyclopropene

2006 ◽  
Vol 131 (1) ◽  
pp. 104-109 ◽  
Author(s):  
Rosa Vilaplana ◽  
M. Carme Valentines ◽  
Peter Toivonen ◽  
Christian Larrigaudière
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Antioxidant Activity ◽  
Storage Period ◽  
Antioxidant Potential ◽  
Total Antioxidant Activity ◽  
Pulp Tissue ◽  
Antioxidant Metabolism ◽  
Beneficial Effects ◽  
Total Antioxidant

In order to determine the effects that 1-methylcyclopropene (1-MCP) may have on antioxidant metabolism during cold storage, apples (Malus ×domestica Borkh. cv. Golden Smoothee) were treated with 625 nL·L−1 1-MCP immediately after harvest and stored in air for 3 months. Differences in total antioxidant activity and ascorbate levels were determined during storage and related to the activity of the antioxidant enzymes superoxide dismutase [SOD (EC 1.15.1.1)], catalase [CAT (EC 1.11.1.6)], and peroxidase [POX (EC 1.11.1.7)] in pulp. The level of oxidative stress in the pulp tissue was also established by determining changes in levels of hydrogen peroxide and in the content of peroxidative markers during storage. Controls and 1-MCP-treated fruit exhibited similar changes in total antioxidant activity and ascorbate levels. However, significant differences in oxidative stress levels were found between treated and untreated fruit. 1-MCP-treated fruit exhibited lower levels of hydrogen peroxide and significantly lower levels in peroxidative markers, especially at the end of the storage period. In line with this last result, 1-MCP-treated fruit also exhibited greater enzymatic antioxidant potential and, more specifically, a higher level of POX activity. Collectively, these results showed that 1-MCP did not detrimentally affect the antioxidant potential of the fruit and provided evidence to support the hypothesis that the beneficial effects of 1-MCP on ripening are not exclusively limited to its effect on ethylene, but also include direct effects on peroxidation and POX enzyme activity.

scholarly journals In-vitro and In-vivo Antioxidant Activity of Ethanol Leaf Extract of Justicia carnea

2020 ◽  
pp. 48-60
Author(s):  
Udedi Stanley Chidi ◽  
Ani Onuabuchi Nnenna ◽  
Asogwa Kingsley Kelechi ◽  
Maduji Fitzcharles Chijindu ◽  
Okafor Clinton Nebolisa
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Superoxide Dismutase ◽  
Leaf Extract ◽  
Dpph Radical ◽  
Β Carotene ◽  
In Vitro Antioxidant Activity

This study investigated the in-vitro antioxidant activity of ethanol leaf extract of Justicia carnea and its effect on antioxidant status of alloxan-induced diabetic albino rats. The in-vitro antioxidant activity was assayed by determining the total phenol, flavonoids, ascorbic acid, β-carotene and lycopene contents and by using 2,2 diphenyl-1-picrylhydrazyl (DPPH) radical, reducing antioxidant power and inhibition of lipid peroxidation antioxidant systems. Oxidative stress was produced in rats by single intraperitoneal injection of 150 mg/kg alloxan and serum concentration of malonaldehyde (MDA), superoxide dismutase (SOD) and catalase (CAT) were determined. Five experimental groups of rats (n=6) were used for the study. Two groups of diabetic rats received oral daily doses of 100 and 200 mg/kg Justicia carnea leaf extract respectively while gilbenclamide (5 mg/ml); a standard diabetic drug was also given to a specific group for 14 days. From the result, the leaf extract contained a higher concentration of flavonoids followed byphenols, ascorbic acid, lycopene and β-carotene. The extract displayed more potent reducing power ability with EC50 of 40 µg/ml compared to BHA (EC50 of 400µg/ml). The percentage DPPH radical scavenging activity of the extract was also higher with EC50 of 200µg/ml and increased with increase in concentration while BHA had EC50of 320µg/ml. The inhibition of lipid peroxidation also increased with increase in concentration with EC50 of 58µg/ml and comparable with BHA (EC50=60µg/ml). The effect of the plant extract on antioxidant enzyme activities was concentration-dependent. Administration of 100mg/kg of the plant extract resulted in a significant decrease (p<0.05) in serum MDA concentration, while 200 mg/kg of the extract caused a significant (p˂0.05) increase in superoxide dismutase (SOD) and catalase activities with a non-significant increase (p>0.05) in the serum level of MDA when compared with the diabetic untreated group. These findings suggest that ethanol leaf extract of Justicia carnea have antioxidant properties and could handle diabetes-induced oxidative stress.

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