scholarly journals Effect of Fermentation of Acacia Leaves (Acacia Mangium) Using Aspergillus Niger on Nutrient Content

2021 ◽  
Vol 3 (2) ◽  
pp. 64
Author(s):  
Sri Sukaryani ◽  
Engkus Ainul Yakin ◽  
Diyah Kartika Wulan

Acacia (Acacia mangium) is a multipurpose plant, useful for fuel, hedge plants, and available throughout the year. Fermentation on acacia leaves needs to be done to reduce crude fiber and increase the dissolved protein content of the feed ingredients. This study aims to determine the effect of the nutritional content of acacia leaves (Acacia magium) fermented using Aspergillus niger as much as 0-2%. The method used in this research is the experimental method of fermenting acacia leaves (Acacia magium). The experimental design used was a completely randomized design (CRD) consisting of three treatments and three repetitions. The treatments were as follows: P0= Fermentation with Aspergillus niger as much as 0% of 300 grams of acacia leaves, P1= Fermentation with Aspergillus niger as much as 1% of 300 grams of acacia leaves, P2= Fermentation with Aspergillus niger as much as 2% of 300 grams of acacia leaves for 7 days . Parameters observed were soluble protein, soluble fat, crude fiber. The average value of crude fat P0: 7.18%, P1: 8.33%, P2:15,47%. The average value of dissolved protein content P0: 2.89%, P1: 2.93%, P2: 3 ,51%. The average value of crude fiber P0: 68.23%, P1: 68.40%, P2: 68.90%. From the results of this study, it can be concluded that Acacia leaves (Acacia Mangium) fermented using Aspergillus niger had a significant effect on crude fat content, but did not significantly affect soluble protein content and crude fiber content of acacia (Acacia Mangium) leaves.

bionature ◽  
2019 ◽  
Vol 19 (2) ◽  
Author(s):  
Darmawan Jamaluddin ◽  
Nurhaeda Nurhaeda ◽  
Rasbawati Rasbawati

Abstract. The purpose of this study was to determine the increase in protein content and decrease in crude fiber in complete feed silage based on rice straw and lamtoro leaves as ruminant feed. This study used a completely randomized design (CRD) with 4 treatment levels and 3 replications namely J0 = 100% rice straw (control), J1 = 60% rice straw and lamtoro leaves 40% J2 = 70% rice straw and lamtoro leaves 30% J3 = 80% rice straw and lamtoro leaves 20%. The results showed that the combination of rice straw and lamtoro leaves had a very significant effect on the value of crude protein and crude fiber in complete feed silage. The highest average to the lowest protein content was J1 (11.45), J2 is equal to (9.50), J3 is equal to (8.44), and J0 is equal to (6.60). The average value of the highest crude fiber content to the lowest is (35.19), J3 is equal to (33.82), J2 is equal to (32.35), and J1is equal to (30.55). From the results of the study it is known that the best treatment that can be recommended is J1 treatment with a combination of 60% rice straw and lamtoro leaves 40%. Keywords: coarse protein, coarse fiber, complete feed silage, lamtoro leaves and rice straw.


2019 ◽  
Vol 4 (2) ◽  
pp. 67
Author(s):  
Ibrahim Hadist

The purpose of this study was to study the interaction between dosages of fungi (T. harzianum & A. niger) and fermentation duration on protein content, crude fiber of bananas corm, and  to determine the best treatment in fermentation. The method used is a Completely Randomized Design (CRD) with  factorial pattern. The first factor (D)  is the dose of fungi (T. harzianum and A. niger): 0.1%; 0.2% and 0.3% and the second factor (L) the fermentation duration: 24 hours, 48 hours, 72 hours and 96 hours. All treatments were replicated two times.  The variables that measured are: protein content and crude fiber content of the material. The data obtained were tested by F test, then Duncan analysis was carried out. The results of the experiment showed  the interaction effect between the both factors on the protein content and the crude fiber of banana weevils. The treatment T. harzianum at a dose of 0.3% and 72 hours of fermentation gave the highest protein content and the lowest crude fiber in bananas corm.Key Word : banana weevil, fermentation,  crude fiber, protein 


2020 ◽  
Vol 2 (2) ◽  
Author(s):  
Dicky Kurniawan ◽  
Sri Sukaryani ◽  
Engkus Ainul Yakin

This study aims to determine the optimal time of fermentation using Aspergillus niger on the pH value, dissolved protein and levels of ash content corn straw. The design used was a Completely Randomized Design (CRD) of unidirectional pattern consisting of 4 treatments repeated 3 times namely P0 : corn straw fermented with Aspergillus niger for 0 days, P1 : corn straw fermented with Aspergillus niger for 4 days, P2 : fermented corn straw with Aspergillus niger for 8 days, P3 : corn straw fermented with Aspergillus niger for 12 days. The parameters observed were pH value, dissolved protein and ash content. The results showed that corn straw fermented with Aspergillus niger had a very significant effect (P <0.01) on the pH value, dissolved protein content and ash content. Corn straw fermentation using Aspergillus niger for 0 - 12 days has a very significant effect on the pH value, dissolved protein content and ash content (P <0.01). The average pH value of the treatment  P0: 5,00, P1: 6,13, P2: 6,51, P3: 6,84 The average value of dissolved protein content in the treatment P0: 27,09%, P1: 33,31%, P2: 24,14%, P3: 25,51%. The average value of ash content in the P0: 8,91%, P1: 11,34%, P2: 10,06%, P3: 9,22%,  As well as the optimal time achieved in fermentation for 4 days for dissolved protein content and ash content.  Keywords: Corn straw, Aspergillus niger, Fermentation, pH value, Dissolved protein content, Ash content


2019 ◽  
Vol 8 (1) ◽  
pp. 48
Author(s):  
Putu Ayu Gaudiya Waisnawi ◽  
Ni Luh Ari Yusasrini ◽  
Putu Timur Ina

The purpose of this study was to determine the ratio effect of suweg flour and mung bean flour to produce the best characteristics of cookies. The Completely Randomized Design was used in this study with a comparison of suweg flour with mung bean flour consisting of 5 levels: 90%: 10%; 80%: 20%; 70%: 30%; 60%: 40%; 50%: 50%  The treatment was repeated 3 times for obtain 15 experimental units. Comparison of suweg flour and mung bean flour has a highly significant effect on fat content and crude fiber content and has a significant effect on protein content and carbohydrate levels of cookies. The comparison ratio of 60% suweg flour and 40% mung bean flour produces cookies with the best characteristics, namely: 1.37% moisture content, 2.29% ash content, 24.44% fat content, 8.89% protein content, carbohydrate content 63.01%, crude fiber content 21.78%, color, aroma, texture crispy, preference and overall acceptance were liked.


2021 ◽  
Vol 1 (2) ◽  
pp. 6-10
Author(s):  
Sri Sukaryani ◽  
Desy Lestari ◽  
Engkus Ainul Yakin

ABSTRAK Kulit kentang merupakan salah satu limbah dapur yang masih bisa dimanfaatkan sebagai pakan ternak, namun disisi lain  kulit kentang jika dimanfaatkan sebagai pakan ternak memiliki nilai gizi yang kurang karena kandungan serat kasar (SK) yang tinggi sehingga perlu dilakukan fermentasi menggunakan Aspergillus niger. Penelitian ini bertujuan untuk mengetahui kadar nutrisi kulit kentang  yang difermentasi menggunakan Aspergillus niger. Desain percobaan menggunakan Rancangan Acak Lengkap (RAL) pola searah dengan 3 perlakuan dan 6 kali ulangan. Data hasil penelitian ini dianalisis menggunakan Analysis of Variance (ANOVA) dan Multiple Range Test (DMRT) pada level signifikansi 5 % & 1 %. Hasil penelitian menunjukan bahwa fermentasi kulit kentang menggunakan Aspergillus niger sebesar 2% berpengaruh nyata terhadap lemak kasar (P<0,05) tetapi tidak berpengaruh nyata terhadap kadar protein terlarut dan kadar serat kasar (P>0,05). Nilai rata-rata protein terlarut pada perlakuan P1: 15,09%, P2: 15,66% dan P3: 17,21%, nilai rata-rata kadar serat kasar pada perlakuan P1: 54,82%, P2: 53,36% dan P3: 52,58%, serta rata-rata kadar lemak kasar pada perlakuan P1: 14,20%, P2: 15,26% dan P3 15.77%. Kesimpulan dari penelitian ini adalah bahwa fermentasi kulit kentang selama 1-3 minggu menggunakan Aspergillus niger sebanyak 2% berpengaruh nyata terhadap lemak kasar, tetapi tidak berpengaruh nyata terhadap kadar protein terlarut dan kadar serat kasar. Namun secara visual kadar protein terlarut mengalami peningkatan serta kadar serat kasar mengalami penurunan. . Kata kunci: Aspergillus niger, fermentasi, kadar nutrisi, kulit kentang ABSTRACT             Potato peel is one of the kitchen wastes that can still be used as animal feed, but on the other hand, potato skins if used as animal feed have less nutritional value because of the high crude fiber content, so it is necessary to ferment it using Aspergillus niger. The purpose of this research was to determine the nutritional content of potato peels fermented using Aspergillus niger. The experimental design used a completely randomized design (CRD) with a unidirectional pattern with 3 treatments and 6 replications. The data from this study were analyzed using Analysis of Variance (ANOVA) and Multiple Range Test (DMRT) at a significance level of 5% & 1%. The results showed that potato peel fermentation using Aspergillus niger at 2% had a significant effect on crude fat (P<0.05) but had no significant effect on soluble protein content and crude fiber content (P>0.05). The average value of dissolved protein in treatment P1: 15.09%, P2: 15.66% and P3: 17.21%, the average value of crude fiber content in treatment P1: 54.82%, P2: 53.36 % and P3: 52.58%, and the average crude fat content in the treatment P1: 14.20%, P2: 15.26% and P3 15.77%. The conclusion of this study was that the fermentation of potato skins for 1-3 weeks using Aspergillus niger as much as 2% had a significant effect on crude fat, but had no significant effect on soluble protein content and crude fiber content. However, visually, the dissolved protein content increased and the crude fiber content decreased. Keywords: Aspergillus niger, fermentation, nutritional content, potato skins  


Author(s):  
Engkus Ainul Yakin ◽  
Ahimsa Kandi Sariri ◽  
Sri Sukaryani

Abstract This research goal is to determine the nutrient content increased and decreased lignin content of cocoa pod fermented by Aspergillus niger. The assessment method was performed using the three treatments and four replications. T0 = cocoa pod fermentation without the addition of Aspergillus niger, T1 = cocoa pod fermentation by the addition of 5% Aspergillus niger, and T2 = cocoa pod fermentation by the addition of 10% of Aspergillus niger. Fresh cocoa pods were chopped to a size of 1-2 cm. Some of the cocoa pods are directly chopped, rolled, and dried while the rest are supplemented with Aspergillus niger. The mixture is stored within an aerobic medium for seven days. Observed variables include dry matter (DM), crude protein (CP), crude fiber (CFt), crude fat (CF), and lignin. Data were analyzed with analysis of variance unidirectional pattern (Oneway ANOVA) of the completely randomized design (completely randomized design), then if there are significant differences will be further tested by Duncan multiple range test. The results showed that cocoa pod fermented with A. niger decrease of dry matter, crude fat, crude fiber, and lignin. The conclusion is the addition of Aspergillus niger 5% will lower the lignin content (5.38%) compared to without the addition of Aspergillus niger (7.84%). Keywords: Aspergillus niger; Cocoa pod; Fermentation; Lignin   Abstrak Penelitian dilakukan untuk mengetahui peningkatan kandungan nutrien dan penurunan kandungan lignin dari kulit buah kakao (KBK) yang difermentasi dengan A. niger. Metode penelitian yang dilakukan yaitu menggunakan tiga perlakuan dan empat ulangan. P0 = KBK fermentasi tanpa penambahan A. niger, P1 = KBK fermentasi dengan penambahan 5% A. niger, dan P2=KBK fermentasi dengan penambahan 10% A. niger . KBK segar  dicacah dengan ukuran 1-2 cm. Sebagian KBK yang dicacah langsung dikeringkan kemudian digiling dan sebagian lainnya diberi penambahan A. niger. Campuran dimasukkan ke dalam wadah aerob selama 7 hari. Penelitian ini dirancang menggunakan desain penelitian rancangan acak lengkap. Variabel yang diamati meliputi kandungan bahan kering (BK), protein kasar (PK), serat kasar (SK), lemak kasar (LK) dan lignin. Data dianalisis menggunakan sidik ragam pola searah (oneway ANOVA) dan dilanjutkan uji jarak berganda Duncan (Duncan multiple range test/DMRT), apabila nilai P<0,05. Hasil penelitian menunjukkan bahwa fermentasi kulit buah kakao dengan A. niger menurunkan kandungan air, lemak kasar, serat kasar dan lignin. Disimpulkan bahwa penambahan A. niger 5% dapat menurunkan kandungan lignin (5,38%) dibandingkan dengan tanpa penambahan A. niger 10% (7,84%). Kata kunci : Aspergillus niger; Fermentasi; Kulit buah kakao; Lignin


2020 ◽  
Vol 9 (4) ◽  
pp. 357
Author(s):  
Lucia Amitasya Sinaga ◽  
Luh Putu Trisna Darmayanti ◽  
I Putu Suparthana

This research aims to determine the comparison effect of mackerel with jackfruit seeds on the characteristics of nuggets, and to find out the exact ratio of it’s to produce the best characteristics of fish nuggets. The completely Randomized Design (CRD) was used in this treatment with mackerel and jackfruit seeds ratio as a treatment consist 6 levels : 100%: 0%; 90%: 10%; 80%: 20 ; 70%: 30%; 60%: 40%; 50%: 50%. Each treatment was repeated 3 times to obtain 18 experimental units. The data obtained were analysis ANOVA and if variance nuggets effect to the variables, followed by The Duncan Multiple Range Test (DMRT). The results showed that the comparison of mackerel and jackfruit seeds give a significant effect to the water content, fat content, protein content, carbohydrate content, crude fiber content, hardness, taste (hedonic), texture (hedonic and scoring), and overall acceptance (hedonic). The 50%: 50% ratio of mackerel with jackfruit seeds produces the best characteristics of the product,contain (32,92% water content, ash content 1,90%, fat content 17,63%, protein content 4,65%, carbohydrate content 42,87%, crude fiber content 10,14%) taste liked, aroma liked, texture and overall acceptance liked.


2019 ◽  
Vol 4 (2) ◽  
pp. 160
Author(s):  
Devy Rahmawati Putri, Agustono, Sri Subekti

Abstract Feed is the element that really support the activities of aquaculture, there for the feed that is available must be adequate and meet the needs of the fish. Efforts to reduce feed costs, some farmers use alternative feed ingredients as a substitute for feed ingredients. Lamtoro leaves in the form of a mixture of flour can be used as fish feed in pellet form. This research been used method with used Completely Randomized Design (CRD) with five treatments and four replications. The treatments used were control (P0), the provision of Probiotics 2% (P1), giving Probiotic 4% (P2), Probiotics administration 6% (P3) and the provision of Probiotics 8% (P4) with each repeated 4 times. Parameters observed were protein content of crude fiber and crude after fermentation for 7 days. These results indicate that administration of probiotics 8% gave significant effect toward crude fiber content in the leaves are fermented and lamtoro which been given Probiotics 6% gave a highly significant effect of the increase in crude protein content in the fermented leaves lamtoro.


2021 ◽  
Vol 10 (2) ◽  
pp. 185
Author(s):  
Yuliana . ◽  
I Desak Putu Kartika Pratiwi ◽  
Ni Made Indri Hapsari Arihantana

Donuts are flour based foods. The weakness of donuts is that the basic ingredients used are still imported, so there is an increase in the use of alternative flour, namely millet flour. The research aims to find out the ratio effect of wheat and millet flour to the characteristic of donut, and to determine the precise ratio between wheat and millet  flour which can produce donuts with the best characteristic. This research used a Completely Randomized Design with a treatmen of ratio between wheat flour and  millet  flour which consist by 6 levels: 100%:0%, 90%:10%, 80%:20%, 70%:30%, 60%:40%, and 50%:50%. The treatment was repeated 3 times to obtain 18 experimental units. The data obtained were analyzed by variance and if treatment had a significant effect then followed by duncan test. The result of research showed that the ratio of 80% wheat and 20%  millet flour resulted in the best characteristic of donut with expand power of 116,69%, colour quantity L 52,77, a* 9,51, b*34,37, water content of 25.88% (wb),  protein content of 8.17% (wb), crude fiber content of  5,06% (wb), tannin content of 0,15% (wb), colour golden yellow,  flavor rather liked, texture rather liked and soft, appearance of rather large pores, taste rather liked and overall acceptance rather liked.


2018 ◽  
Vol 7 (4) ◽  
pp. 175
Author(s):  
I Gusti Agung Ayu Sintia Padma Dewi ◽  
I Gusti Ayu Ekawati ◽  
I Desak Putu Kartika Pratiwi

The purpose of this research was to know the effect of germination time of millet (Panicum milliaceum) on the characteristics of flakes and to know the right germination time of millet on the characteristics of flakes. The experimental design used was completely randomized design with the germination time of millet treatment, namely 24 hours (T1), 48 hours (T2), 72 hours (T3), 96 hours (T4), and 120 hours (T5). The treatment was repeated 3 repetitions then obtained 15 units of the experiment, the data obtained from the result were analyzed by variance and if the treatment had an effect on the variable then continued with Duncan test. The results showed that the germination of millet had significant effect to water content, ash content, protein content, fat content, crude fiber content, tannin content, taste level (hedonic test), and overall acceptance of flakes. 120 hours germination time of millet had the best characteristics with water content 1,36%, ash content 2,65%, protein content 9,93%, fat content 7,86%, carbohydrate content 78,20%, crude fiber content 5,93, tannin content 0,31%, color liked, flavor liked, texture crispy and liked, taste liked  and overall acceptance liked.


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