scholarly journals Observing the Anti-oxidant and Anti-inflammatory Effect of Nigella Sativa Combined With Silybum Marianum Extracts on the Acute Peritonitis Mouse Model

2021 ◽  
Vol 24 (3) ◽  
pp. 372-385
Author(s):  
Maryam Bahrami ◽  
◽  
Ali Ghazavi ◽  
Ali Ganji ◽  
Ghasem Mosayebi ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Therapeutic Potential ◽  
Inflammatory Diseases ◽  
Nigella Sativa ◽  
Control Group ◽  
Silybum Marianum ◽  
Acute Peritonitis ◽  
Anti Oxidant ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Background and Aim: In addition to free radicals such as Nitric Oxide (NO), inflammation is one of the most important pathophysiological causes of peritonitis. Over thousands of years, Nigella Sativa (NS) and Silybum Marianum (SM) are two plants known for their anti-oxidant and anti-inflammatory properties. However, the effect of its compound is unclear. Thus, in this study, we evaluated the anti-inflammatory effect of NS and SM extracts and their combination on inflammatory diseases like thioglycollate peritoneal. Methods & Materials: Alcoholic extracts of SM and NS were obtained by the soxhlet method. Male Balb/C mice were divided into 5 groups and gavage orally for 14 days with SM, NS, the mixture of extracts of these two, DMSO 30% as the control group, and dexamethasone as the positive control group. The safety profile and acute toxicity in mice were assessed. On day 10, acute peritonitis was induced by thioglycollate 3%. Finally, the total anti-oxidant power and NO concentration were measured by FRAP and Griess method, respectively, in the serum of treated mice. Ethical Considerations: All experimental process was performed following the guidelines according to the Animal Ethics Committee of Arak University of Medical Sciences (IR.ARAKMU.REC.1397.359). Results: Acute toxicity test showed no significant changes in weight and physical appearance of the mice. However, the extract and their mixture decreased NO level significantly (P=0.000) in serum. Also, the mixture significantly increased total anti-oxidant power (P=0.015). Conclusion: Results showed that the SM and NS extract mixture demonstrated anti-inflammatory activity, inhibiting inflammatory mediators such as NO and increasing anti-oxidant power, thus supporting its therapeutic potential in slowing down inflammatory processes in inflammation disorders.

Anti-inflammatory Effect of Alcoholic Extract of Nigella sativa L on Bovine Fibroblast-like synoviocyte and THP-1

2018 ◽  
Vol 9 (02) ◽  
pp. 20181-20191 ◽  
Author(s):  
Dr Maghsoudi, Hossein ◽  
Samaneh Haj-allahyari
Keyword(s):  
Nigella Sativa ◽  
Pcr Analysis ◽  
Control Group ◽  
Alcoholic Extract ◽  
Iranian Traditional Medicine ◽  
Tnf Α ◽  
Radiocarpal Joint ◽  
Cox 2 ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Due to the side effects of current therapies for osteoarthritis one of the alternative medicine is using herbal medicine such as Nigella sativa .L which in Iranian traditional medicine has been used as a treatment option. The purpose of this study is evaluating the effect of alcoholic extract of Nigella sativa (AENS) on pro-inflammatory cytokines in Bovine Fibroblast-like BFLs (BFLS) and THP-1. BFLS cells were isolated from Radiocarpal joint. After evaluating of LC50 (27 µg/mL), both cells (5x105 (cells\wel)) were incubated at 37 ° C and 5% CO2 and 90% humidity for 72 hours with AENS (6.13 μg\ml as a media LC50). One set of cells was activated for 1h with LPS for RT-PCR analysis of COX-2, INOS, IL-1β, TNF-α expression and another set of cells was activated for 24h, cells supernatant were analyzed for PGE-2 and nitrite content. The present study demonstrates that AENS reduced expression levels of COX-2, INOS, TNF-α in control group. Reduced expression of COX2 and INOS was significantly along with the reducing production of NO and PGE2.Also, AENS decreased the expression of TNF-α and iL-1β in control group. Our results showed that the anti-inflammatory effect of AENS not only has anti-inflammatory effect on the BFLS cells but also related to the THP-1 that are active in the synovial membrane.

scholarly journals Nigella Sativa’s Anti-Inflammatory and Antioxidative Effects in Experimental Inflammation

Antioxidants ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 921 ◽  
Author(s):  
Raluca Maria Pop ◽  
Octavia Sabin ◽  
Șoimița Suciu ◽  
Stefan Cristian Vesa ◽  
Sonia Ancuța Socaci ◽  
...  
Keyword(s):  
Acute Phase ◽  
Acute Inflammation ◽  
Antioxidant Activities ◽  
Nigella Sativa ◽  
Control Group ◽  
Acute Administration ◽  
Inflammatory Conditions ◽  
Experimental Inflammation ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Nigella sativa (NS) has been used for centuries in various inflammatory conditions because of its anti-inflammatory and antioxidant activities. The study aimed to evaluate the anti-inflammatory, antinociceptive and antioxidant activity of Nigella sativa oil (NSO) in two models of acute (carrageenan-induced) and sub-acute inflammation (complete Freund’s adjuvant induced) in rats. Materials and Methods: NSO was administered orally 1, 2 and 4 mL/kg in the acute phase. For subacute phase, NSO was administered 4 mL/kg, 7 days before or after inflammation induction, or in association with diclofenac 5 mg/kg. Results: The gas chromatography coupled with mass spectroscopy (GC-MS) analysis showed that NSO is an important source of bioactive compounds, especially p-cymene and thymoquinone. In the acute phase, 1.5 h after administration, NSO (2 and 4 mL/kg) determined an anti-inflammatory effect comparable with that of diclofenac. In the sub-acute administration, NSO had no anti-inflammatory effect. The analgesic effect of NSO was observed only in the sub-acute inflammation in the analgesy-meter test. NSO as treatment proved its antioxidant effect through the reduction of malondialdehyde (MDA) and oxidized glutathione (GSSG), and increases in hydrogen donor capacity (DH) compared to the control group, but the effect was not as intense as that of diclofenac. Conclusion: The present study has proven inconstant anti-inflammatory, analgesic and antioxidative properties of NSO.

In vivo anti-inflammatory effect and toxicological screening of Maytenus heterophylla and Maytenus senegalensis extracts

2010 ◽  
Vol 30 (7) ◽  
pp. 693-700 ◽  
Author(s):  
G. da Silva ◽  
M. Taniça ◽  
J. Rocha ◽  
R. Serrano ◽  
ET Gomes ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Inflammatory Diseases ◽  
Inflammatory Activity ◽  
Albino Rats ◽  
Toxicity Screening ◽  
Leaf Extracts ◽  
Maytenus Senegalensis ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Maytenus heterophylla (Eckl & Zeyh.) Robson and Maytenus senegalensis (Lam). Exell are two African medicinal plants used to treat painful and inflammatory diseases. We evaluated the in vivo (per os) anti-inflammatory activity of M. heterophylla leaf, stem and root extracts and of M. senegalensis leaf and stem extracts. Additionally, we assessed their in vivo acute and sub-acute toxicities. Anti-inflammatory activities of ethanol extracts were determined in Wistar albino rats, by the carrageenan-induced paw oedema method. Acute and sub-acute toxicity screening of the extracts was evaluated in adult male CD-6 mice. Leaf extracts of M. heterophylla and M. senegalensis exhibited significant anti-inflammatory activity (120 mg/kg, per os), reducing oedema by 51% and 35%, respectively. While M. heterophylla extracts at 1200 mg/kg have shown to be non-toxic, M. senegalensis extracts indicated some toxicity. Our results show a significant anti-inflammatory effect of both M. heterophylla and M. senegalensis leaf extracts in a local model of acute inflammation and suggest the absence of acute and sub-acute toxicity signs of the M. heterophylla leaf extract (but not of M. senegalensis). Ongoing studies will surely shed some light into the mechanism of action of this active extract and establish its chemical fingerprint.

Anti-oxidant and anti-inflammatory effect of polar extracts obtained from waste product of wine making

2020 ◽  
pp. 1-5
Author(s):  
M. J. Germanó ◽  
M. D. Muñoz ◽  
M. C. Della-Vedova ◽  
G. E. Feresin ◽  
M. Rinaldi-Tosi ◽  
...  
Keyword(s):  
Waste Product ◽  
Wine Making ◽  
Anti Oxidant ◽  
Anti Inflammatory ◽  
Polar Extracts ◽  
Inflammatory Effect

MAPK/AP-1-Targeted Anti-Inflammatory Activities of Xanthium strumarium

2016 ◽  
Vol 44 (06) ◽  
pp. 1111-1125 ◽  
Author(s):  
Muhammad Jahangir Hossen ◽  
Mi-Yeon Kim ◽  
Jae Youl Cho
Keyword(s):  
Mouse Model ◽  
Therapeutic Potential ◽  
Inflammatory Diseases ◽  
Elevated Serum ◽  
Human Monocyte ◽  
Serum Levels ◽  
Mitogen Activated Protein Kinase ◽  
Xanthium Strumarium ◽  
U937 Cells ◽  
Anti Inflammatory

Xanthium strumarium L. (Asteraceae), a traditional Chinese medicine, is prescribed to treat arthritis, bronchitis, and rhinitis. Although the plant has been used for many years, the mechanism by which it ameliorates various inflammatory diseases is not yet fully understood. To explore the anti-inflammatory mechanism of methanol extracts of X. strumarium (Xs-ME) and its therapeutic potential, we used lipopolysaccharide (LPS)-stimulated murine macrophage-like RAW264.7 cells and human monocyte-like U937 cells as well as a LPS/D-galactosamine (GalN)-induced acute hepatitis mouse model. To find the target inflammatory pathway, we used holistic immunoblotting analysis, reporter gene assays, and mRNA analysis. Xs-ME significantly suppressed the up-regulation of both the activator protein (AP)-1-mediated luciferase activity and the production of LPS-induced proinflammatory cytokines, including interleukin (IL)-1[Formula: see text], IL-6, and tumor necrosis factor (TNF)-[Formula: see text]. Moreover, Xs-ME strongly inhibited the phosphorylation of mitogen-activated protein kinase (MAPK) in LPS-stimulated RAW264.7 and U937 cells. Additionally, these results highlighted the hepatoprotective and curative effects of Xs-ME in a mouse model of LPS/D-GalN-induced acute liver injury, as assessed by elevated serum levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT), and histological damage. Therefore, our results strongly suggest that the ethnopharmacological roles of Xs-ME in hepatitis and other inflammatory diseases might result from its inhibitory activities on the inflammatory signaling of MAPK and AP-1.

Medicinal Herbs with Anti-Inflammatory Activities for Natural and Organic Healing

2021 ◽  
Vol 25 ◽  
Author(s):  
Mohamad Hesam Shahrajabian
Keyword(s):  
Herbal Medicine ◽  
Medicinal Plants ◽  
Cannabis Sativa ◽  
Inflammatory Diseases ◽  
Nigella Sativa ◽  
Centella Asiatica ◽  
Medicinal Herbs ◽  
Scientific Publications ◽  
Anti Inflammatory ◽  
Clinical Experiments

: One of the principal causes of different disorders is an uncontrolled inflammatory response. Alkaloids, flavonoids, polyphenolic, proanthocyanidin, terpenoid, and steroid compounds are the main reasons for the anti-inflammatory activities of medicinal herbs and plants. The current manuscript introduces a series of potential anti-inflammatory plants, particularly those which are routines in Iranian and Chinese traditional herbal medicine, and simplifies the function and mechanisms of natural constituents for the prevention and treatment of inflammatory disorders. PubMed, Science Direct, Google Scholar, Wiley Online Library, Springer, Taylor, Francis, etc., have been used to search for collecting of scientific publications for a full evaluation of current documentation in the literature showing the importance of medicinal plants with anti-inflammatory characteristics and natural medicines. The most notable medicinal plants with anti-inflammatory activities are Baccharis dracunculifolia, Aconitum bulleyanum, Crateya adansonii, Alliums spp., Centella asiatica, Flos lonicerae, Corydalis dubia, Syringae folium, Coptis chinensis, Casearia decandra, Nigella sativa, Cannabis sativa, Tamarindus indica L., Glycyrrhiza glabra, Panax ginseng, Panax notoginseng, Pistacia vera, Smilax china, Scutellaria baicalensis, Rosemarinus officinalis, Moringa olifera, Pulsatilla radix, Pistacia atlantica, Rullia tuberose, Canarium album, Dodonaea polyandra, Forsythia suspense, Polygala tenuifolia, Radiz Isatidis, Hypericum sampsonii, Geranium koreanum, Typha capensis, Isatidis folium, Ginkgo biloba, Houttuynia cordata, snow lotus, etc. Herbal medicine mainly uses numerous parts of plants or combinations of them to prevent and remedy diseases and promote health. More investigations and clinical experiments are needed to provide more information on the importance of medicinal plants as well as their roles in the treatment and prevention of inflammatory diseases.

Pharmacological activities and Therapeutic potential of kaempferitrin in medicine for the treatment of human disorders: A review of medicinal importance and health benefits

2021 ◽  
Vol 21 ◽  
Author(s):  
Dinesh Kumar Patel
Keyword(s):  
Broiler Chickens ◽  
Therapeutic Potential ◽  
Scientific Information ◽  
Aloe Vera ◽  
Crocus Sativus ◽  
Related Complication ◽  
Food Material ◽  
Biological Potential ◽  
Anti Oxidant ◽  
Anti Inflammatory

Background: Herbal drugs and their derived phytochemicals are valuable for human being as a source of vital component of food material and drugs. Flavonoids are naturally occurring phytochemical produced in plants through metabolisms and they are having anti-hyperlipidemia, anti-inflammatory, anti-oxidant and anti-apoptotic activity. Flavonoids have been identified in the fruits, nuts, vegetables, seeds, stem, flowers and tea. Kaempferol is a natural flavonoidal compound present in edible plants such as apples, broccoli, strawberries, beans, grapefruit, propolis and medicinal plants such as Aloe vera, Ginkgo biloba, Rosmarinus officinalis, Crocus sativus L., Hypericum perforatum L. Kaempferol have anti-oxidant, anti-inflammatory, anti-apoptotic, pro-apoptotic, cardio-protective and anti-cancer activities. Methods: Glycosides of kaempferol such as kaempferitrin also called kaempferol 3,7-dirhamnoside are known to be more abundant than their flavonoid monomers in plants. Various literature databases have been searched to collect all the scientific information of kaempferitrin in the present investigation and analyzed in order to know the therapeutic benefit and biological potential of kaempferitrin. Moreover all the information has been presented here in two broad sections i.e. pharmacological and analytical. Results: From the analysis of all the collected and presented information, it was found that kaempferitrin has potent insulin-mimetic potential and could be used for the treatment of diabetes and related complication. However, it has also shown anti-oxidant, anti-inflammatory, anti-convulsant, anti-osteoporotic, anti-depressant, anthelmintic, immunostimulatory and natriuretic properties and inhibits cell proliferation and apoptosis. Kaempferitrin also improves meat quality of broiler chickens. Conclusions: The presented information in this work will be valuable to justify the biological importance and therapeutic potential of kaempferitrin in the scientific field.

scholarly journals Investigation of Pharmacological Activity of Caralluma penicillata: Anti-Inflammatory Properties and Gastritis Protection against Indomethacin in Adult Guinea Pigs

2014 ◽  
Vol 2014 ◽  
pp. 1-9
Author(s):  
Nabil Albaser ◽  
Najeeb Ghanem ◽  
Mohanad Shehab ◽  
Adnan Al-Adhal ◽  
Mohammed Amood AL-Kamarany
Keyword(s):  
Chronic Inflammation ◽  
Guinea Pigs ◽  
Therapeutic Potential ◽  
Histological Study ◽  
Folk Medicine ◽  
Ethanol Extract ◽  
Optimum Dose ◽  
Repeated Doses ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Caralluma is a plant that possessing a great therapeutic potential in folk medicine in Yemen, namely, Caralluma penicillata (C. penicillata) as antiulcer. The study aims to evaluate the anti-inflammatory properties and gastritis protection activity of C. penicillata against indomethacin in adult guinea pigs. The study was divided into four parts: firstly, the optimum dose of extract as anti-inflammatory effect was determined. Secondly, the acute anti-inflammatory effect of extract were estimated. Thirdly, the repeated doses of extract against chronic inflammation was estimated. The anti-inflammatory activity of extract was compared with indomethacin as a prototype of drug against inflammation. Fourthly, the gastritis protection properties of extract with/without indomethacin were performed. The results showed that a 400 mg/kg of 10% ethanol extract produced the maximum of anti-inflammatory effect. Also, the single dose of extract was equipotent for indomethacin (10 mg/kg), but shorter in duration with regard to acute anti-inflammatory effect. In addition, the repeated doses of extract against chronic inflammation were less potent than indomethacin with regard to ulcerogenic effect. On the other hand, extract-indomethacin combination reduced the gastritis effect of indomethacin based on ulcer index and histological study.

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