Green Synthesis of Silver Nanoparticles using Ajuga parviflora Benth and Digera muricata Leaf extract: Their Characterization and Antimicrobial Activity

The aim of the present study is to search out nontoxic silver nanoparticles synthesized from the leaf extract of two plants Ajuga parviflora Benth and Digera muricata for antimicrobial activity. The plants used in this investigation are rich in alkaloids, flavonoids, steroids, terpenoids, protein, amino acids, carbohydrate, quninones, phenols and tannins. The formation of nanoparticles were confirmed by UV/Visible spectroscopy, peaks at 423nm for Ajuga parviflora Benth and 408nm for Digera muricata. The morphology of the silver nanoparticles was established through state of the art spectroscopic tools. SEM analysis reveals average size of AgNPs 18 nm for Digera muricata and 22 nm for Ajuga parviflora Benth respectively while transmission electron microscopy confirms that AgNPs are spherical in shape. The synthesized nanoparticles were subjected to Escherichia coli, Staphylococcus aureus, Salmonella typhimurium and Pseudomonas aeruginosa. The results suggest that the silver nanoparticles have promising activity against all the bacterial strains and can be used an effective bactericides.

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SILVER NANOPARTICLE IMPREGNATED BIO-BASED ACTIVATED CARBON WITH ENHANCED ANTIMICROBIAL ACTIVITY

International Journal of Nanoscience ◽

10.1142/s0219581x13500245 ◽

2013 ◽

Vol 12 (04) ◽

pp. 1350024 ◽

Cited By ~ 1

Author(s):

R. SELVAKUMAR ◽

S. P. SURIYARAJ ◽

V. JAYAVIGNESH ◽

K. SWAMINATHAN

Keyword(s):

Antimicrobial Activity ◽

Silver Nanoparticles ◽

Yeast Cells ◽

X Ray Diffraction ◽

Gram Positive ◽

Biological Reduction ◽

Gram Negative ◽

Transmission Electron ◽

Gram Negative Organisms ◽

Average Size

The present study involves the production of silver nanoparticles using a novel yeast strain Saccharomyces cerevisiae BU-MBT CY-1 isolated from coconut cell sap. The biological reduction of silver nitrate by the isolate was deducted at various time intervals. The yeast cells after biological silver reduction were harvested and subjected to carbonization at 400°C for 1 h and its properties were analyzed using Fourier transform infra-red spectroscopy, X-ray diffraction, scanning electron microscope attached with energy dispersive spectroscopy and transmission electron microscopy. The average size of the silver nanoparticles present on the surface of the carbonized silver containing yeast cells (CSY) was 19 ± 9 nm. The carbonized control yeast cells (CCY) did not contain any particles on its surface. The carbonized silver nanoparticles containing yeast cells (CSY) were made into bioactive emulsion and tested for its efficacy against various pathogenic Gram positive and Gram negative bacteria. The antimicrobial activity studies indicated that CSY bioactive nanoemulsion was effective against Gram negative organisms than Gram positive organism.

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Biosynthesis of silver nanoparticles using lavender leaf and their applications for catalytic, sensing, and antioxidant activities

Nanotechnology Reviews ◽

10.1515/ntrev-2016-0041 ◽

2016 ◽

Vol 5 (6) ◽

Cited By ~ 11

Author(s):

Brajesh Kumar ◽

Kumari Smita ◽

Luis Cumbal

Keyword(s):

Silver Nanoparticles ◽

Leaf Extract ◽

Antioxidant Activities ◽

Present Report ◽

X Ray Diffraction ◽

Visible Spectroscopy ◽

X Ray ◽

Transmission Electron ◽

Red Color ◽

Uv Visible

AbstractThe present report summarizes an eco-friendly approach for the biosynthesis of silver nanoparticles (AgNPs) using the leaf extract of lavender. Initially, the synthesis of AgNPs was visually observed by the appearance of a wine red color. The optical property, morphology, and structure of as-synthesized AgNPs were characterized by UV-visible spectroscopy, dynamic light scattering, transmission electron microscopy, and X-ray diffraction analyses. All characterization data revealed the formation of crystalline and spherical AgNPs (Ag/Ag

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Biosynthesis and Characterisation of Silver Nanoparticles using Leaf Extract of Achras sapota l. for its Antimicrobial Activity

International Journal of Scientific Research in Science and Technology ◽

10.32628/ijsrst207548 ◽

2020 ◽

pp. 246-256

Author(s):

Maphibanri Maring ◽

Akila Elias ◽

V. B. Narayanaswamy

Keyword(s):

Antimicrobial Activity ◽

Silver Nanoparticles ◽

Leaf Extract ◽

Colour Change ◽

Visible Spectroscopy ◽

Sem And Tem ◽

Achras Sapota ◽

Uv Visible ◽

Biosynthesized Agnps ◽

Ethanolic Leaf Extract

Nanotechnology is a field that is rapidly growing, making an impact in all spheres of human life. In the current study, silver nanoparticles were synthesized using the ethanolic leaf extract of Achras sapota. Characterization was carried out using UV-Visible spectroscopy, FTIR, XRD, SEM and TEM. The formation of AgNPs was confirmed through UV-Visible spectroscopy by the colour change. Based on the XRD pattern, the crystalline property of the AgNPs was established. The functional group present in the ethanolic leaf extract of Achras sapota is responsible for the reduction of the Ag+ ion which was studied through FTIR. From the SEM and TEM analysis, it was found that the formed nanoparticles are spherical in shape and nano in size. The biosynthesized AgNPs was evaluated for its antimicrobial activity against gram positive bacteria (S. aureus and L. bacillus) and gram negative bacteria (E. coli and P. aeruginosa) using disc diffusion for preliminary screening of antimicrobial activity and dilution method for evaluation of antibacterial effectiveness and effect of silver nanoparticles on bacterial growth and it was found to exhibit potential antimicrobial activity. The biosynthesized AgNPs was found to be efficient in terms of reaction time as well as stability, eco-friendly and cost effective.

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Synthesis, characterization and antibacterial potential of silver nanoparticles by Morus nigra leaf extract

Indian Journal of Pharmaceutical and Biological Research ◽

10.30750/ijpbr.1.4.4 ◽

2013 ◽

Vol 1 (04) ◽

pp. 16-24 ◽

Cited By ~ 12

Author(s):

Anu Kumar ◽

Kuldeep Kaur ◽

Sarika Sharma

Keyword(s):

Silver Nanoparticles ◽

Leaf Extract ◽

Bacterial Species ◽

Nitrate Solution ◽

Average Particle Size ◽

Silver Nitrate Solution ◽

Sem Analysis ◽

Average Particle ◽

Morus Nigra ◽

Uv Visible

The present study reports the synthesis of silver nanoparticle using Morus nigra leaf extract were used as reducing agent for reduction of silver nitrate solution. The synthesis of silver nanoparticles was analyzed by UV-Visible spectroscopy, Scanning Electron Microscopy. The SEM analysis has shown that size of silver nanoparticles synthesized from leaves extract of M.nigra was 200 nm and seems to be spherical in morphology. Morphology of chemically synthesized silver nanoparticles is nearly spherical and of size ranges from 300-500 nm. The average particle size analyzed from SEM analysis was observed to be 350 nm. This article has discussed the synthesis of silver nanoparticles generated from plant extract, characterization and antibacterial analysis. In this study the antibacterial activity was examined against six MTCC cultures collected from IMTECH Chandigarh, Including both gram positive and gram negative bacteria such as P.aeruginosa, S.aureus, B.subtilis, E.coli, P.flourescens and Streptococus mutans. Out of these strains the antimicrobial activity of the silver nanoparticles showed maximum zone of inbhition against P.flourescens (22 mm), P.aeruginosa (19 mm), S.aureus (18 mm) and least effective against E.coli (15mm). In contrast chemically synthesized silver nanoparticles were found most effective against S.aureus (13 mm) and B.subtilis (12mm) and almost ineffective against Streptococcus mutans (6 mm) and P.flourescens (4 mm). In the concluding remarks, the silver nanoparticles synthesized using M.nigra leaves extract would be a better antimicrobial effective against various bacterial species.

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Manufacturing Technique and Antimicrobial Activity of Silver Nanoparticles

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.365-366.1169 ◽

2013 ◽

Vol 365-366 ◽

pp. 1169-1172

Author(s):

An Pang Chen ◽

Ching Wen Lou ◽

Ya Yuan Chung ◽

Mei Chen Lin ◽

Jia Horng Lin

Keyword(s):

Antimicrobial Activity ◽

Silver Nanoparticles ◽

Antibacterial Agent ◽

Rapid Development ◽

Reaction System ◽

Medical Application ◽

Development Stage ◽

Particle Sizes ◽

Transmission Electron ◽

Uv Visible

Recently, the biomaterial is in rapid development stage, which could be widely applying in medical application, due to its biodegradability, nontoxic and biocompatibility. Chitosan is naturally abundant polymers have the biodegradability, nontoxic and biocompatibility. In this research, the chitosan and silver nitrate were used to develop the antibacterial agent via nanotechnique. In the reaction system, the reaction time and stirring speed were discussed, which will affect the surface plasmon resonance. The particle sizes were measured using transmission electron microscopy (TEM) and UV visible spectrophotometry. The silver nanoparticles size was below 100 nm via TEM. In addition, the results of antimicrobial activity indicated that the antibacterial agent has well antimicrobial activity on staphylococcus aureus. Due to the silver nanoparticle provides relatively higher surface area to contact with bacteria affect DNA molecules by losing their replication abilities.

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Biosynthesis and characterization of Gynocardia odorata R. Br. mediated silver nanoparticles and evaluation of its antimicrobial activity

Plant Science Today ◽

10.14719/pst.2021.8.4.1217 ◽

2021 ◽

Vol 8 (4) ◽

Author(s):

Dipjyoti Kalita ◽

Himanshu Rajbongshi ◽

Nilakshee Devi ◽

Mohan Chandra Kalita ◽

Sunandan Baruah

Keyword(s):

Silver Nanoparticles ◽

Ostwald Ripening ◽

Bacterial Strains ◽

X Ray Diffraction ◽

Ag Nps ◽

Optical Absorption Band ◽

Good Antibacterial Activity ◽

Transmission Electron ◽

Uv Visible

The present study was designed to synthesize Silver Nanoparticles (Ag-NPs) in aqueous medium using leaf extract of Gynocardia odorata R. Br. (Achariaceae). The synthesized Ag-NPs were characterized using different technique such as UV-Visible Spectroscopy, X-Ray Diffraction (XRD) Spectroscopy, Fourier Transform Infrared Spectroscopy (FTIR) and Transmission Electron Microscopy (TEM). The reduction of Ag ions to initiate nucleation and subsequent Ostwald Ripening to form nanoparticles was made possible by the presence of various antioxidants in the leaves of Gynocardia odorata. These antioxidants served both as reducing and capping agents. The synthesized Ag-NPs were found to be polydispersed in nature and spherical in shape. With the Surface Plasmon Resonance (SPR) optical absorption band peak at ~440 nm was observed using UV-Vis spectrophotometer. FTIR confirmed the presence of methoxy and allyl groups in the synthesized Ag-NPs and nearly 15-45 nm diameter spherical shaped NPs was validated using TEM. The synthesized Ag-NPs were stable for a long period (more than six months) and showed good antibacterial activity against both gram positive and gram negative bacterial strains and the effect was higher as compared to the normal aqueous extract.

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Green Synthesis of Silver Nanoparticles by UsingZiziphus nummulariaLeaves Aqueous Extract and Their Biological Activities

Journal of Nanomaterials ◽

10.1155/2016/8026843 ◽

2016 ◽

Vol 2016 ◽

pp. 1-8 ◽

Cited By ~ 10

Author(s):

Farhat Ali Khan ◽

Muhammad Zahoor ◽

Abdul Jalal ◽

Aziz Ur Rahman

Keyword(s):

Silver Nanoparticles ◽

Ag Nanoparticles ◽

Leaf Extract ◽

Biological Activities ◽

Antioxidant Properties ◽

Antioxidant Property ◽

Sem Analysis ◽

Scanning Calorimetry ◽

Minimum Fungicidal Concentration ◽

Uv Visible

Silver nanoparticles ofZiziphus nummularialeaves extract were synthesized and were characterized by UV-Visible spectrophotometry, particle size analyzer, X-ray diffraction (XRD), differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FT-IR), SEM, TGA, and EDX. The XRD pattern reveals the FCC structure of Ag nanoparticles. FTIR spectra confirmed the presence of Ag-O bonding. UV-Visible spectroscopy results confirmed the existence of Ag because of the particular peak in the region of 400–430. The SEM analysis confirmed spherical and uniform Ag nanoparticles with diameter ranging from 30 nm to 85 nm. The EDX analysis revealed strong signals in the silver region and confirmed the formation of silver nanoparticles. The antioxidant potential and antifungal and antimicrobial potential of the leaf extract and silver nanoparticles were also determined. The antioxidant property was determined using DPPH assay. The antibacterial, antifungal, and antioxidant properties were better for the silver nanoparticles than the aqueous leaf extract. The minimum inhibitory concentration (MIC), minimum bactericidal (MBC), and minimum fungicidal concentration (MFC) of plant extract and prepared silver nanoparticles were also tested. The hair growth properties of plant extracts and their respective nanoparticles were observed and good results were noted for nanoparticles as compared to the leaf extract.

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Green Synthesis of Silver Nanoparticles by Allamanda cathartica L. Leaf Extract and Evaluation for Antimicrobial Activity

International Journal of Pharmaceutical Sciences and Nanotechnology ◽

10.37285/ijpsn.2013.6.4.9 ◽

2013 ◽

Vol 6 (4) ◽

pp. 2260-2268

Author(s):

M. Linga Rao ◽

Bhumi G ◽

Savithramma N

Keyword(s):

Antimicrobial Activity ◽

Silver Nanoparticles ◽

Leaf Extract ◽

Fusarium Species ◽

Inhibition Zone ◽

Fungal Species ◽

Green Technology ◽

Metallic Silver ◽

Synthesis Of Nanoparticles ◽

Aqueous Leaf Extract

Silver nanoparticles (SNPs) exhibit tremendous applications in medicine as antimicrobial agent. The use of different parts of plants for the synthesis of nanoparticles is considered as a green technology as it does not involve any harmful chemicals. In the present study, we report a rapid biosynthesis of silver nanoparticles from aqueous leaf extract of medicinal plant Allamanda cathartica. The active phytochemicals present in the plant were responsible for the quick reduction of silver ion to metallic silver nanoparticles. The reduced silver nanoparticles were characterized by using UV-Vis spectrophotometry, Scanning Electron Microscope (SEM), Energy Dispersive Analysis of X-ray (EDAX) and Atomic Force Microscopy (AFM). The spherical shaped silver nanoparticles were observed and it was found to 19-40 nm range of size. These phytosynthesized SNPs were tested for their antimicrobial activity and it analyzed by measuring the inhibitory zone. A. cathartica aqueous leaf extract of SNPs showed highest toxicity to Pseudomonas followed by Klebsiella, Bacillus and E. coli and lowest toxicity towards Proteus. In fungal species, highest inhibition zone was noted against Rhizopus followed by Curvularia, Aspergillus flavus and Aspergillus niger and minimum inhibition zone was observed against Fusarium species. These results suggest a promising potential of Indian plant-based green chemistry for production of SNPs for biomedical and nanotechnology applications.

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Antimicrobial Activity of Silver Nanoparticles Synthesized by Streptomyces Species JF714876

International Journal of Pharmaceutical Sciences and Nanotechnology ◽

10.37285/ijpsn.2012.5.1.7 ◽

2012 ◽

Vol 5 (1) ◽

pp. 1638-1642 ◽

Cited By ~ 2

Author(s):

Vidyasagar G M ◽

Shankaravva B ◽

R Begum ◽

Imrose ◽

Sagar R ◽

...

Keyword(s):

Antimicrobial Activity ◽

Silver Nanoparticles ◽

Silver Ions ◽

Human Beings ◽

Streptomyces Species ◽

Force Microscopy ◽

E Coli ◽

Extracellular Synthesis ◽

Atomic Force ◽

Uv Visible

Microorganisms like fungi, actinomycetes and bacteria are considered nanofactories and are helpful in the production of nanoparticles useful in the welfare of human beings. In the present study, we investigated the production of silver nanoparticles from Streptomyces species JF714876. Extracellular synthesis of silver nanoparticles by Streptomyces species was carried out using two different media. Silver nanoparticles were examined using UV-visible, IR and atomic force microscopy. The size of silver nanoparticles was in the range of 80-100 nm. Antimicrobial activity of silver nanoparticle against bacteria such as E. coli, S. aureus, and dermatophytes like T. rubrum and T. tonsurans was determined. Thus, this study suggests that the Streptomyces sp. JF741876 can produce silver ions that can be used as an antimicrobial substance.

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