scholarly journals An automatic Time Table Generation

2021 ◽  
pp. 831-835
Author(s):  
KV Prasad Reddy ◽  
B. Thandava Krishna ◽  
T. Nithin Sai ◽  
YT. Surekha ◽  
G. Kowsalya ◽  
...  
Keyword(s):  
Automated System ◽  
Computer Assisted ◽  
Time Table ◽  
Simple Interface

The manual system of preparing time table in colleges is very time consuming and Monotonous task which usually ends up with various classes clashing either at identical room or with same teachers having more than one class at a time. To slove all these problems we developing an automated system with computer assisted timetable generator. This system provides a simple interface for generating the timetable automatically. It can be used by educational institutes or colleges to view their timetable in most efficient and easy manner.

144 Dynamic of Sperm Subpopulations in Red Deer Capacitated Samples

2018 ◽  
Vol 30 (1) ◽  
pp. 212
Author(s):  
M. Ramón ◽  
M. Iniesta-Cuerda ◽  
A. Martín-Maestro ◽  
P. Peris-Frau ◽  
I. Sánchez-Ajofrín ◽  
...  
Keyword(s):  
Red Deer ◽  
Lateral Displacement ◽  
Semen Analysis ◽  
Sperm Head ◽  
Change Points ◽  
Computer Assisted ◽  
Change Over Time ◽  
Flagellar Beat ◽  
Time Table ◽  
Over Time

An ejaculate is a mixture of sperm subpopulations (SP) with varying motility characteristics. Moreover, males with high percentages of fast and linear-moving sperm have high rates of fertility (Ramón et al. 2003 Biol. Reprod. 89, 110). The objective was to assess dynamics, over time, of SP of capacitated red deer sperm. Thawed sperm were selected with 45/90% Percoll, diluted at 10 × 106 sperm mL−1 in SOF plus 10% oestrous sheep serum and incubated for 2 h at 38.5°C under 5% CO2. Sperm motility was assessed by computer-assisted semen analysis at 1, 5, 15, 30, 45, 60, and 120 min and 24 h. Sperm were classified as described previously (Martínez-Pastor et al. 2005 Biol. Reprod. 72, 316-327) and the evolution of SP during capacitation was characterised with piece-wise regression that identified change points. Five sperm SP were identified based on velocity according to an actual path (VCL), velocity according to a straight path (VSL), velocity according to the average, smoothed path (VAP), linearity (LIN), straightness (STR), wobble (WOB), amplitude of lateral displacement of sperm head (ALH), and frequency of the flagellar beat (BCF). Sperm in SP1 were fast, linear sperm with high ALH; they corresponded to capacitated sperm. In contrast, SP5 were slow, non-linear sperm, with low ALH (Table 1). The dynamics of each SP differed over time was different along the time. Percentages of SP1, SP4, and SP3 were significantly decreased at 60, 90, and 100 min, whereas percentages of SP2 and SP5 did not change over time. This study was consistent with previous reports that kinematic sperm characteristics change over time. Table 1.Sperm subpopulations (SP) based on kinematic end points.

scholarly journals Computer-assisted automatic synthesis II. Development of a fully automated apparatus for preparing substituted N–(carboxyalkyl)amino acids

1989 ◽  
Vol 11 (5) ◽  
pp. 212-220 ◽  
Author(s):  
Nobuyoshi Hayashi ◽  
Tohru Sugawara ◽  
Motoaki Shintani ◽  
Shinji Kato
Keyword(s):  
Artificial Intelligence ◽  
Amino Acids ◽  
Average Rate ◽  
Kinetic Equations ◽  
Pharmaceutical Research ◽  
Substituent Effects ◽  
Automated System ◽  
Computer Assisted ◽  
Optimum Reaction ◽  
The Many

A versatile automated apparatus, equipped with an artificial intelligence has been developed which may be used to prepare and isolate a wide variety of compounds. The prediction of the optimum reaction conditions and the reaction control in real time, are accomplished using novel kinetic equations and substituent effects in an artificial intelligence software which has already reported [1]. This paper deals with the design and construction of the fully automated system, and its application to the synthesis of a substituted N-(carboxyalkyl)amino acid. The apparatus is composed of units for perfoming various tasks, e.g. reagent supply, reaction, purification and separation, each linked to a control system. All synthetic processes including washing and drying of the apparatus after each synthetic run were automatically performed from the mixing of the reactants to the isolation of the products as powders with purities of greater than 98%. The automated apparatus has been able to run for 24 hours per day, and the average rate of synthesis of substituted N-(carboxyalkyl)amino acids has been three compounds daily. The apparatus is extremely valuable for synthesizing many derivatives of one particular compound structure. Even if the chemical yields are low under the optimum conditions, it is still possible to obtain a sufficient amount of the desired product by repetition of the reaction. Moreover it was possible to greatly reduce the manual involvement of the many syntheses which are a necessary part of pharmaceutical research.

scholarly journals Approach to Improving the Automated System Computer Analysis of the Electrocardiogram

2019 ◽  
pp. 42-48
Author(s):  
Viktor Vladimirovich Muromtsev ◽  
Valeriy Mikhailovich Nikitin ◽  
Olga Alekseevna Efremova ◽  
Lyudmila Aleksandrovna Kamyshnikova
Keyword(s):  
Computer Analysis ◽  
Main Idea ◽  
Automated System ◽  
Computer Assisted ◽  
Time Warping ◽  
Interactive Tools ◽  
Analysis System ◽  
Dynamic Time ◽  
Electrocardiogram Ecg ◽  
Degree Of Similarity

Purpose is to develop and test an approach to the improvement of automated electrocardiogram (ECG) computer analysis systems, which increases physician productivity. Materials and methods. The existing prototype of the ECG computer analysis system developed by the authors is used as materials. Dynamic time warping (DTW) method, as well as structural and object-oriented software development techniques were used. Results and discussion. The main idea of improving automated computer-assisted ECG analysis systems is to provide the doctor with convenient software tools that allow you to: set various templates for QRS complexes; select templates and automatically find QRS complexes in ECG that are most similar to the selected template. The main result of the work is an algorithm that allows to quantify the degree of similarity of a QRS complex isolated from an ECG and a given pattern. The algorithm is based on the DTW method. The proposed improvement was tested when modifying the existing ECG computer analysis system. As a result of the modification, convenient interactive tools were added to the system, which allow the doctor to navigate the QRS complexes while viewing the ECG, taking into account their degree of similarity to the selected template. This gave the doctor the opportunity to quickly assess the patient’s condition and make a conclusion. Conclusion. The proposed approach allows you to modify the automated computer-aided analysis of the ECG in order to increase the productivity of the doctor.

12 FERTILITY OF FROZEN EQUINE SPERM IN SYSTEMS FOR CRYOPRESERVATION

2012 ◽  
Vol 24 (1) ◽  
pp. 117
Author(s):  
R. R. D. Maziero ◽  
P. N. Guasti ◽  
I. D. P. Blanco ◽  
I. Martin ◽  
G. A. Monteiro ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Liquid Nitrogen ◽  
Membrane Integrity ◽  
Skim Milk ◽  
Egg Yolk ◽  
Automated System ◽  
Epifluorescence Microscopy ◽  
Computer Assisted ◽  
Plasma Membrane Integrity ◽  
Sperm Analysis

Optimizing cryopreservation of equine sperm will facilitate genetic banking and propagation of important horse strains through assisted reproduction. This study aimed to evaluate the motility pattern using computer-assisted sperm analysis (CASA) and plasma membrane integrity by epifluorescence microscopy of equine semen frozen in 0.5 mL straws at different freezing rates; also, a fertility trial was performed according to the freezing protocol. Three ejaculates from four stallions of various breeds (Mangalarga Marchador, Westfallen, Hanovarian and Arabian) and ages (5 to 20 years) were collected and processed for cryopreservation. The stallions were housed at the CERBEQ, Reproduction Centre of the Department of Animal Reproduction and Veterinary Radiology, UNESP. The ejaculates were filtered and submitted to analysis by CASA (HTM IVOS 12, Hamilton Thorne Research, USA). In addition, the plasma membrane integrity was determined by fluorescent probes. After evaluation, the ejaculates were diluted at 1:1 (extender:semen) with skim milk extender Botu-Semen™ and centrifuged at 600 × g for 10 min. The supernatant was removed and the pellet resuspended to a final concentration of 100 × 106 sperm mL–1 with milk-egg yolk freezing extender (Botu-Crio™). Semen was packaged in 0.5-mL straws (IMV, LAigle, France) and was placed in nitrogen for 20 min and then from room temperature to 5°C and then frozen in two different cooling systems: an isothermic box (42 cm × 28 cm × 12.5 cm) was placed upon racks suspended 6 cm above liquid nitrogen or other 20 min then immersed into nitrogen and automated system Mini Digitcool™ (IMV Technologies, France), cooling at a –40°C min–1 rate. All straws were stored in liquid nitrogen until thawing and analysis. The straws were thawed in a water bath at 46°C for 20 s and the samples were evaluated for progressive motility, angular progressive velocity, progressive velocity, track speed, percentage of rapid sperm and percentage of sperm with plasma membrane integrity. For the fertility trial, 65 clinically healthy mares had their oestrous cycle monitored by ultrasound and inseminated postovulation with sperm into the uterus. Ovulation was induced with 1 mL of deslorelin acetate (GnRH) injected IM when a 35-mm follicle was detected. Thirty-six hours later, mares were monitored every 6 h until ovulation was detected. When it was detected, mares were inseminated with 800 × 106 total sperm. Pregnancy was confirmed via ultrasound examination 15 days after ovulation. Pregnancy rate was 52.2% using the isothermic box and 60% using the automated machine. Statistical analysis from the frozen–thawed semen evaluated parameters was performed using the statistics software Proc. MIXED of SAS 9.1 and for the fertility trial, logistic regression using the Proc GENMOD from SAS 9.1. The conventional method using the isothermic box was similar to the automated machine with a fast freezing rate. Additionally, AI with 800 × 106 sperm frozen in the isothermic box or automated system resulted in similarly acceptable conception rates.

Controlled Self-Assembly of Collagen Fibrils by an Automated Dialysis System

2006 ◽  
Vol 128 (5) ◽  
pp. 792-796 ◽  
Author(s):  
Stefan Strasser ◽  
Albert Zink ◽  
Wolfgang M. Heckl ◽  
Stefan Thalhammer
Keyword(s):  
Self Assembly ◽  
Collagen Type ◽  
Diffusion Rate ◽  
Collagen Fibrils ◽  
The Self ◽  
Automated System ◽  
Computer Assisted ◽  
Type I ◽  
Assembly Process ◽  
Self Assembled

In vitro self-assembled collagen fibrils form a variety of different structures during dialysis. The self-assembly is dependent on several parameters, such as concentrations of collagen and α1-acid glycoprotein, temperature, dialysis time, and the acid concentration. For a detailed understanding of the assembly pathway and structural features like banding pattern or mechanical properties it is necessary to study single collagen fibrils. In this work we present a fully automated system to control the permeation of molecules through a membrane like a dialysis tubing. This allows us to ramp arbitrary diffusion rate profiles during the self-assembly process of macromolecules, such as collagen. The system combines a molecular sieving method with a computer assisted control system for measuring process variables. With the regulation of the diffusion rate it is possible to control and manipulate the collagen self-assembly process during the whole process time. Its performance is demonstrated by the preparation of various collagen type I fibrils and native collagen type II fibrils. The combination with the atomic force microscope (AFM) allows a high resolution characterization of the self-assembled fibrils. In principle, the represented system can be also applied for the production of other biomolecules, where a dialysis enhanced self-assembly process is used.

Quantification of Capillary Density and Inter-Capillary Distance in Nailfold Capillary Images Using Scale Space Capillary Detection and Ordinate Clust

2017 ◽  
Vol 6 (1) ◽  
pp. 32-49
Author(s):  
K. V. Suma ◽  
Bheemsain Rao
Keyword(s):  
Clustering Algorithm ◽  
Visual Analysis ◽  
Capillary Density ◽  
Scale Space ◽  
Automated System ◽  
Computer Assisted ◽  
Space Construction ◽  
Sensitivity Specificity ◽  
Very High

The visual analysis of Nailfold Capillary images manually requires trained medical staff and also, the intra-observer variations can be very high. A computer assisted capillary analysis reduces this burden to a great extent. The authors propose an automated system using advanced techniques such as Scale Space construction using Anisotropic Diffusion and Ordinate clustering algorithm. The classification of capillaries is evaluated on the basis of Sensitivity, Specificity and Classification Accuracy. The effectiveness of anisotrpic filtering and Ordinate clustering in eliminating erroneous detection is demonstrated. The capillary density and inter-capillary distance are important capillary parameters which can contribute to the diagnosis of different diseases.

3-D organization of fibrinogen receptors using computer reconstruction and whole-mount microscopy

1988 ◽  
Vol 46 ◽  
pp. 30-31
Author(s):  
E. T. O'Toole ◽  
R. R. Hantgan ◽  
J. C. Lewis
Keyword(s):  
Whole Blood ◽  
Colloidal Gold ◽  
Blood Platelets ◽  
Cell Contact ◽  
Computer Assisted ◽  
Adherent Cells ◽  
Differential Centrifugation ◽  
Computer Reconstruction ◽  
Sds Page ◽  
Whole Mount

Thrombocytes (TC), the avian equivalent of blood platelets, support hemostasis by aggregating at sites of injury. Studies in our lab suggested that fibrinogen (fib) is a requisite cofactor for TC aggregation but operates by an undefined mechanism. To study the interaction of fib with TC and to identify fib receptors on cells, fib was purified from pigeon plasma, conjugated to colloidal gold and used both to facilitate aggregation and as a receptor probe. Described is the application of computer assisted reconstruction and stereo whole mount microscopy to visualize the 3-D organization of fib receptors at sites of cell contact in TC aggregates and on adherent cells.Pigeon TC were obtained from citrated whole blood by differential centrifugation, washed with Ca++ free Hank's balanced salts containing 0.3% EDTA (pH 6.5) and resuspended in Ca++ free Hank's. Pigeon fib was isolated by precipitation with PEG-1000 and the purity assessed by SDS-PAGE. Fib was conjugated to 25nm colloidal gold by vortexing and the conjugates used as the ligand to identify fib receptors.

Computer Assisted Image Reconstruction of Oligomer Structure

1976 ◽  
Vol 34 ◽  
pp. 472-473
Author(s):  
A.M. Jones ◽  
A. Max Fiskin
Keyword(s):  
Three Dimensional ◽  
Depth Of Field ◽  
Computer Assisted ◽  
Projection Data ◽  
Two Dimensional ◽  
Single Particles ◽  
Dimensional Reconstruction ◽  
Computer Assisted Image ◽  
The Fourier Transform ◽  
Space Approach

If the tilt of a specimen can be varied either by the strategy of observing identical particles orientated randomly or by use of a eucentric goniometer stage, three dimensional reconstruction procedures are available (l). If the specimens, such as small protein aggregates, lack periodicity, direct space methods compete favorably in ease of implementation with reconstruction by the Fourier (transform) space approach (2). Regardless of method, reconstruction is possible because useful specimen thicknesses are always much less than the depth of field in an electron microscope. Thus electron images record the amount of stain in columns of the object normal to the recording plates. For single particles, practical considerations dictate that the specimen be tilted precisely about a single axis. In so doing a reconstructed image is achieved serially from two-dimensional sections which in turn are generated by a series of back-to-front lines of projection data.

Biodegradable polyester neuroprostheses promote the reconnection of separated peripheral nerve stubs

1992 ◽  
Vol 50 (2) ◽  
pp. 1094-1095
Author(s):  
Beverly L. Giammara ◽  
Jennifer S. Stevenson ◽  
Peggy E. Yates ◽  
Robert H. Gunderson ◽  
Jacob S. Hanker
Keyword(s):  
Image Analysis ◽  
Basement Membrane ◽  
Periodic Acid ◽  
Light And Electron Microscopy ◽  
Computer Assisted ◽  
Type Iii Collagen ◽  
Image Analysis System ◽  
Biodegradable Polyester ◽  
Adult Rats ◽  
Type Iv

An 11mm length of sciatic nerve was removed from 10 anesthetized adult rats and replaced by a biodegradable polyester Vicryl™ mesh sleeve which was then injected with the basement membrane gel, Matrigel™. It was noted that leg sensation and movement were much improved after 30 to 45 days and upon sacrifice nerve reconnection was noted in all animals. Epoxy sections of the repaired nerves were compared with those of the excised segments by the use of a variation of the PAS reaction, the PATS reaction, developed in our laboratories for light and electron microscopy. This microwave-accelerated technique employs periodic acid, thiocarbohydrazide and silver methenamine. It stains basement membrane or Type IV collagen brown and type III collagen (reticulin), axons, Schwann cells, endoneurium and perineurium black. Epoxy sections of repaired and excised nerves were also compared by toluidine blue (tb) staining. Comparison of the sections of control and repaired nerves was done by computer-assisted microscopic image analysis using an Olympus CUE-2 Image Analysis System.

High-resolution measurement of birefringent fine structure in living cells using a new polarized light microscope

1995 ◽  
Vol 53 ◽  
pp. 54-55
Author(s):  
Rudolf Oldenbourg
Keyword(s):  
Light Microscope ◽  
Fluorescent Dyes ◽  
Polarized Light ◽  
Processing System ◽  
Video Camera ◽  
Molecular Organization ◽  
Computer Assisted ◽  
Image Recording ◽  
Birefringent Crystal ◽  
Technological Advances

The recent renaissance of the light microsope is fueled in part by technological advances in components on the periphery of the microscope, such as the laser as illumination source, electronic image recording (video), computer assisted image analysis and the biochemistry of fluorescent dyes for labeling specimens. After great progress in these peripheral parts, it seems timely to examine the optics itself and ask how progress in the periphery facilitates the use of new optical components and of new optical designs inside the microscope. Some results of this fruitful reflection are presented in this symposium.We have considered the polarized light microscope, and developed a design that replaces the traditional compensator, typically a birefringent crystal plate, with a precision universal compensator made of two liquid crystal variable retarders. A video camera and digital image processing system provide fast measurements of specimen anisotropy (retardance magnitude and azimuth) at ALL POINTS of the image forming the field of view. The images document fine structural and molecular organization within a thin optical section of the specimen.

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