Effect of plant growth-promoting bacteria and inoculation media in the yield on cooksfoot yield

Objective: To determine the effect of three PGPB bacteria (Ewingella americana, Bacillus simplex and Microbacterium ginsengiterrae) and three inoculation media (digestate, compost and cornstarch) on plant height, leaf temperature, stalkdensity, morphological composition, and dry matter yield of orchard grass.Design/Methodology/Approach: A completely randomized design was used, with a factorial arrangement 4X3, the experimental units being a pot with five initial stalks.Results: The dry matter did not show difference between treatments with bacteria (P>0.05), but the control the values increased up to 50%. Compost and digestate media outperformed cornstarch by 22%. The inoculation media had an effect(P?0.05) on the yield and leaf variables, while the bacteria promoted the formation of new stalks and the production of biomass, and reduced the leaf temperature.Findings/Conclusions: Compost and digestate as inoculation media can notably favor the beneficial effect of M. ginsengiterrae, which was the one that registered the best yields

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Comparative Effects of Bio-Wastes in Combination with Plant Growth-Promoting Bacteria on Growth and Productivity of Okra

Agronomy ◽

10.3390/agronomy11102065 ◽

2021 ◽

Vol 11 (10) ◽

pp. 2065

Author(s):

Hammad Anwar ◽

Xiukang Wang ◽

Azhar Hussain ◽

Muhammad Rafay ◽

Maqshoof Ahmad ◽

...

Keyword(s):

Plant Growth ◽

Soil Health ◽

Organic Amendments ◽

Plant Growth Promoting Rhizobacteria ◽

Fruit Weight ◽

Growth And Yield ◽

Plant Growth Promoting Bacteria ◽

Randomized Design ◽

Plant Growth Promoting ◽

Growth Promoting

Plant growth-promoting rhizobacteria with multiple growth-promoting traits play a significant role in soil to improve soil health, crop growth and yield. Recent research studies have focused on the integration of organic amendments with plant growth-promoting rhizobacteria (PGPR) to enhance soil fertility and reduce the hazardous effects of chemical fertilizers. This study aims to evaluate the integrated application of biochar, compost, fruit and vegetable waste, and Bacillus subtilis (SMBL 1) to soil in sole application and in combined form. The study comprises eight treatments—four treatments without inoculation and four treatments with SMBL 1 inoculation in a completely randomized design (CRD), under factorial settings with four replications. The results indicate that the integrated treatments significantly improved okra growth and yield compared with sole applications. The integration of SMBL 1 with biochar showed significant improvements in plant height, root length, leaf chlorophyll a and b, leaf relative water content, fruit weight, diameter and length by 29, 29, 50, 53.3, 4.3, 44.7 and 40.4%, respectively, compared with control. Similarly, fruit N, P and K contents were improved by 33, 52.7 and 25.6% and Fe and Zn in shoot were 37.1 and 35.6%, respectively, compared with control. The results of this study reveal that the integration of SMBL 1 with organic amendments is an effective approach to the sustainable production of okra.

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Influence of Ethanolic extract of Aspilia Africana leaf on the performance and egg qualities of Japanese quails

Global Journal of Pure and Applied Sciences ◽

10.4314/gjpas.v24i2.2 ◽

2018 ◽

Vol 24 (2) ◽

pp. 135-140

Author(s):

O.O.K Oko ◽

P.O Ozung ◽

F.B Abang

Keyword(s):

Ethanolic Extract ◽

Feed Conversion ◽

Final Body Weight ◽

Randomized Design ◽

Old Japanese ◽

Egg Composition ◽

Growth Promoting ◽

Completely Randomized Design ◽

The One ◽

One Way Anova

A 16-week trial evaluated the effects of ethanolic extract of Aspiliaafricana leaf (EeAaL) on the growth performance, carcass and egg characteristics of growing and laying quails. One hundred and eighty (180), one-week old Japanese quail chicks were assigned to six experimental diets (0, 2.5, 5, 7.5, 10% EeAaL or 0.02% oxytetracycline). Each treatment had 30 birdswhich were further subdivided into three replicates of 10 birds. Each replicate was housed in a pen under the deep litter system; feed and water were provided ad libitum.Growth performance and egg characteristics(egg quantity and quality) indices were subjected to the one-way ANOVA in a completely randomized design (CRD). No significant (P>0.05) effect of EeAaL was observed between treatments on the final body weight and daily weight gains of quails. Feed conversion ratio (4.95 – 5.28) was significantly (P<0.05) improved in quails fed 2.5 – 7.5% EeAaL diets. Zero mortality was observed in quails fed 5 – 10% EeAaL diets compared to other treatments. EeAaL supplementation reduced (P<0.05) the amount of fat deposition in quail carcass, thus improving the carcass quality. The efficacy ofEeAaL was more pronounced in laying than growing quails where significant (P<0.05) differences were observed on some external and internal egg characteristics, whereas egg composition did not differ (P>0.05) between treatments. Egg number, hen day production, albumen weight,yolk weight, shell thickness and yolk colour were significantly improved following dietary EeAaL upplementation, indicative of strong growth promoting and stimulatory effects.Therefore, for improved carcass and egg performances up to 7.5% EeAaL shoud be supplemented intoquail diets.Keywords: Leaf extract, growth rate, egg characteristics, bush marigold, quails

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Identification of Canola Roots Endophytic Bacteria and Analysis of Their Potential as Biofertilizers for Canola Crops with Special Emphasis on Sporulating Bacteria

Agronomy ◽

10.3390/agronomy11091796 ◽

2021 ◽

Vol 11 (9) ◽

pp. 1796

Author(s):

Pilar Martínez-Hidalgo ◽

José David Flores-Félix ◽

Fernando Sánchez-Juanes ◽

Raúl Rivas ◽

Pedro F. Mateos ◽

...

Keyword(s):

Endophytic Bacteria ◽

Paenibacillus Polymyxa ◽

Plant Growth Promoting Bacteria ◽

Microcosm Experiment ◽

Brassica Napus L ◽

Bacillus Simplex ◽

Paenibacillus Sp ◽

Plant Growth Promoting ◽

Growth Promoting ◽

Hydroponic Conditions

Canola (Brassica napus L. var. oleracea) is the third most common oil-producing crop worldwide after palm and soybean. Canola cultivation requires the use of chemical fertilizers, but the amount required can be reduced by applying plant growth-promoting bacteria (PGPB). Among PGPB, endophytic bacteria have certain advantages as biofertilizers, but canola endophytic bacteria have rarely been studied. In this work, we identified a collection of bacterial endophytes isolated from canola roots using MALDI-TOF MS, a technique that is still rarely used for the identification of such bacteria, and rrs gene sequencing, a methodology that is commonly used to identify canola endophytes. The results demonstrated that some bacterial isolates from canola roots belonged to the genera Bacillus, Neobacillus, Peribacillus (Pe.), and Terribacillus, but most isolates belonged to the genera Paenibacillus (P.) and Pseudomonas (Ps.). Inoculation of these isolates indicated that several of them could efficiently promote canola seedling growth in hydroponic conditions. These results were then confirmed in a microcosm experiment using agricultural soil, which demonstrated that several isolates of Pseudomonas thivervalensis, Paenibacillus amylolyticus, Paenibacillus polymyxa, Paenibacillus sp. (Paenibacillus glucanolyticus/Paenibacillus lautus group), and Peribacillus simplex (previously Bacillus simplex) could efficiently promote canola shoot growth under greenhouse conditions. Among them, the isolates of Paenibacillus and Peribacillus were the most promising biofertilizers for canola crops as they are sporulated rods, which is an advantageous trait when formulating biofertilizers.

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Effectiveness of Mycorrhiza, Plant Growth Promoting Rhizobacteria and Inorganic Fertilizer on Chlorophyll Content in Glycine max (L.) cv. Detam-4 Prida

E3S Web of Conferences ◽

10.1051/e3sconf/202122600031 ◽

2021 ◽

Vol 226 ◽

pp. 00031

Author(s):

Muhammad Muhammad ◽

Umi Isnatin ◽

Peeyush Soni ◽

Praptiningsih Gamawati Adinurani

Keyword(s):

Plant Growth ◽

Chlorophyll Content ◽

Plant Growth Promoting Rhizobacteria ◽

Nitrogen And Phosphorus ◽

Randomized Design ◽

Effective Combination ◽

Plant Growth Promoting ◽

Glycine Max L ◽

Growth Promoting ◽

Completely Randomized Design

This study aimed to find an effective combination of mycorrhiza, PGPR (Plant Growth Promoting Rhizobacteria), and inorganic fertilizers. Whereas the specific purpose was observed in effective mycorrhiza to increased chlorophyll content. This study used a completely randomized design (CRD) with three factors: the 1st factor is mycorrhiza application, the 2nd factor is PGPR and the 3rd factor is the application of nitrogen and phosphorus fertilizers. The data were analyzed with Analysis of Variance to determine the effect of the treatment being tried. Continued with the Least Significance Different test at a 95 % confidence level. The results indicated that the most effective application in increasing total chlorophyll content was (i) the ‟Commercial Mycorrhiza” without being combined with PGPR and fertilizer (TP.TR.MP:80 g mL–1). (ii) ‟Brawijaya Mycorrhiza” is combined with PGPR without fertilizer (TP.R.MB: 83 g mL–1). (iii) ‟Unida Mycorrhiza” without combined with PGPR and without fertilizer (TP.TR.MU: 80 g mL–1).

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PLANT GROWTH PROMOTING BACTERIA EFFECTS ON DRY MATTER YIELD IN HIGH ELEVATION RANGELANDS OF TURKEY

Turkish Journal Of Field Crops ◽

10.17557/tjfc.357423 ◽

2017 ◽

Author(s):

Mahmut DAŞCI ◽

N. Zeynep YILDIRIM ◽

M. Kerim GÜLLAP ◽

Binali ÇOMAKLI ◽

Ramazan ÇAKMAKÇI ◽

...

Keyword(s):

Plant Growth ◽

Dry Matter ◽

High Elevation ◽

Plant Growth Promoting Bacteria ◽

Dry Matter Yield ◽

Plant Growth Promoting ◽

Growth Promoting

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APLIKASI RHIZOBAKTERI PEMACU TUMBUH (RPT) DARI AKAR TITONIA (Tithonia diversifolia) TERHADAP PERTUMBUHAN STEK MELATI (Jasminum officinale) PADA ULTISOL

Jurnal Solum ◽

10.25077/jsolum.15.2.75-82.2018 ◽

2018 ◽

Vol 15 (2) ◽

pp. 75

Author(s):

Agustian Agustian ◽

Anisah Permata Sari ◽

Lusi Maira

Keyword(s):

Plant Height ◽

Plant Growth Promoting Rhizobacteria ◽

Tithonia Diversifolia ◽

Branch Number ◽

Randomized Design ◽

Plant Growth Promoting ◽

Number Of Leaves ◽

Growth Promoting ◽

Completely Randomized Design

Jasmine plant is a horticultural crop that has promising prospect to be developed in trading. The development of jasmine cultivation is determined by the quality of seedling that can be produced rapidly for planting in the field. The purpose of this research is to study the effect of application of Plant growth-promoting rhizobacteria (PGPR) from titonia root (Tithonia diversifolia) in increasing the growth of jasmine cuttings (Jasminum officinale). This experiment used Completely Randomized Design (RAL) which consists of 7 treatments with 5 replications. The treatments were: A (control), B (Growtone), C (Growtone + 1 ml PGPR), D (Growtone + 2 ml PGPR), E (Growtone + 4 ml PGPR) , F (Growtone + 6 ml PGPR) and G (Growtone + 8 ml PGPR). Data obtained were analyzed statistically with F test at 5% level. The results showed that the application of PGPR can increase the growth of jasmine cuttings. However, the increase in PGPR dose did not have a significant effect on the increase of leaf number, branch number and height of jasmine cuttings. The highest number of leaves, branch and plant height at two months seedling age were found in treatment (C) 1 ml PGPR with 48.60 leaves, 5.80 branch and 22.90 cm plant height that significantly different from other treatments,. Treatment (D) 2 ml PGPR application increased the population of P solubilizing bacteria in rhizosphere from 2.59x104 cfu/g of soil at control to 5.05x105 cfu/g soil and fixing bacteria N (Azospirillium) 3x104 cfu/g soil to 1.41x105 cfu/g soil and Azotobacter to 1.2x 103 cfu/g soil.Key words : Jasmine, PGPR, cuttings, Ultisol

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COMPATIBILITY OF AZOXYSTROBIN AND CYPROCONAZOLE ON PLANT GROWTH-PROMOTING MICROORGANISMS

JOURNAL OF NEOTROPICAL AGRICULTURE ◽

10.32404/rean.v7i4.5281 ◽

2020 ◽

Vol 7 (4) ◽

pp. 95-100

Author(s):

Weslany Silva Rocha ◽

Mara Caroline Alves da Silva ◽

Gilberto Coutinho Machado Filho ◽

Mauro Gomes Dos Santos ◽

Aloísio Freitas Chagas Júnior ◽

...

Keyword(s):

Plant Growth ◽

Bradyrhizobium Japonicum ◽

Integrated Management ◽

Growth Promoter ◽

Randomized Design ◽

Plant Growth Promoting ◽

Agroecosystem Management ◽

Growth Promoting ◽

Completely Randomized Design ◽

Set Up

Avoiding the toxicity effect of chemical fungicides on rhizobacteria is a sustainable alternative for agroecosystem management. Rhizobacteria, whose bioprotective and plant growth-promoter potential have been reported in the literature, lack studies on their performance in integrated management with pesticides. Thus, this study aimed to evaluate the effects of azoxystrobin + cyproconazole on the growth of Bacillus subtilis, Streptomyces seoulensis, and Bradyrhizobium japonicum. Three independent experiments were set up, i.e., one for each microorganism, and carried out in a completely randomized design in a factorial scheme (3 × 6), with three doses (recommended by the manufacturer, half, and twice) and six periods of evaluations (48, 96, 144, 192, 240, and 288 hours), with three replications. Growth inhibition rings were evaluated. Azoxystrobin + cyproconazole at all tested doses is compatible with B. subtilis. The use of azoxystrobin + cyproconazole affected the growth of B. japonicum and S. seoulensis, which were sensitive to all its doses until 288 hours after inoculation.

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Isolation and characterization of two plant growth-promoting bacteria from the rhizoplane of a legume (Lupinus albescens) in sandy soil

Revista Brasileira de Ciência do Solo ◽

10.1590/s0100-06832010000200009 ◽

2010 ◽

Vol 34 (2) ◽

pp. 361-369 ◽

Cited By ~ 15

Author(s):

Adriana Giongo ◽

Anelise Beneduzi ◽

Adriana Ambrosini ◽

Luciano Kayser Vargas ◽

Marcos Roberto Stroschein ◽

...

Keyword(s):

Plant Growth ◽

Dry Matter ◽

Nifh Gene ◽

Plant Growth Promoting Bacteria ◽

Bacterial Strains ◽

Indolic Compounds ◽

Isolation And Characterization ◽

Plant Growth Promoting ◽

Growth Promoting

Two bacterial strains that amplified part of the nifH gene, RP1p and RP2p, belonging to the genus Enterobacter and Serratia, were isolated from the rhizoplane of Lupinus albescens. These bacteria are Gram-negative, rod-shaped, motile, facultative anaerobic, and fast-growing; the colonies reach diameters of 3-4 mm within 24 h of incubation at 28 ºC. The bacteria were also able to grow at temperatures as high as 40 ºC, in the presence of high (2-3 % w/v) NaCl concentrations and pH 4 -10. Strain RP1p was able to utilize 10 of 14 C sources, while RP2p utilized nine. The isolates produced siderophores and indolic compounds, but none of them was able to solubilize phosphate. Inoculation of L. albescens with RP1p and RP2p strains resulted in a significant increase in plant dry matter, indicating the plant-growth-promoting abilities of these bacteria.

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Bacillus spp. as plant growth-promoting bacteria in cotton under greenhouse conditions

Australian Journal of Crop Science ◽

10.21475/ajcs.19.13.12.p2003 ◽

2019 ◽

pp. 2003-2014

Author(s):

Paola Andrea Escobar Diaz ◽

Noemi Carla Baron ◽

Everlon Cid Rigobelo

Keyword(s):

Plant Growth ◽

Dry Matter ◽

Phosphorus Content ◽

Good Alternative ◽

Fertilizer Application ◽

Plant Growth Promoting Bacteria ◽

P Efficiency ◽

Promising Alternative ◽

Plant Growth Promoting ◽

Growth Promoting

The use of plant growth-promoting bacteria (PGPB) is a promising alternative method to improve plant efficiency in the utilization of chemical fertilizers, enabling a reduction of fertilizer application on crops. This study aimed to evaluate the potential of ten Bacillus strains (eight B. subtilis, one B. velezensis and one B. amyloliquefaciens) to promote growth in cotton plants under greenhouse conditions. The experiment was performed in a completely randomized design with 11 treatments and five replicates under greenhouse conditions. The parameters related to plant growth from treatments that received the bacterial isolates were compared to the control. The parameters analyzed were shoot dry matter, root dry matter, total dry matter, plant height, nitrogen content and phosphorus content in soil and in plants. The highest root dry matter was 1.24 g for the isolate 263. The total dry matter was 4.0 g for the isolate 248 and 3.54 g for the isolate 290. The highest chlorophyll content was 28 µg/cm2 for the isolate 290. The higher N content in shoot dry matter was 28 g of N for the isolate 290, 26 g for the isolate 248 and 25 g for the isolate 320. The improved P efficiency use was 32% for the isolate 248, 28% for the isolate 188 and 27% for the isolate 274. These results strongly confirm that B. subtilis isolates 248, 290 and 263 may represent a good alternative as plant growth-promoting endophytes to cotton crops, as they positively affected several parameters evaluated, such as root and shoot dry matter and phosphorus content in the soil. In addition, the parameters evaluated can strongly and positively affect plant yield. However, some isolates of B. subtilis did not promote plant growth and most likely failed as bioinoculants. This result shows the importance of properly identifying the isolate for bioinoculation to achieve success in promoting plant growth.

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Stomata dan Trikoma Kultivar Kedelai Anjasmoro selama pemupukan Nanosilika dan Plant Growth Promoting Rhizobacteria

Vegetalika ◽

10.22146/veg.48795 ◽

2020 ◽

Vol 9 (1) ◽

pp. 343

Author(s):

Sri Suryanti ◽

Arif Umami

Keyword(s):

Plant Growth ◽

Plant Growth Promoting Rhizobacteria ◽

Nano Silica ◽

Randomized Design ◽

Silica Concentration ◽

The Status ◽

Plant Growth Promoting ◽

Growth Promoting ◽

Completely Randomized Design ◽

Soybean Plants

Soybean is a very important crop commodity in Indonesia. Increasing soybean production on dryland as cultivation land need to be carried out. Dryland have issues with either of nutrient and water availability which is not supportable for plant growth. Therefore, the application of nano-silica and plant growth promoting rhizobacteria (PGPR) are expected to increase nutrient absorption, synthesis of plant growth hormone and increase in plant resistance to drought stress. The growth of drought-resistant plants is able to be showed through the status of stomata and trichomes on leaves. So that, this study aimed to determine the stomata and trichome performance of soybean plants of Anjasmoro cultivar using nano-silica and PGPR as fertilizers. The study design used a completely randomized design with 2 factors with three replication. The first factor was nano silica concentration i.e. 0, 100 and 200 ppm. The second factor was PGPR concentration i.e. 0, 5, 10, and 15%. Results showed that the nanosilica and PGPR applications had no significant effect on stomata and trichome status of Anjasmoro cultivar except for stomatal aperture and the ratio of the size of the stomata openings. A positive correlation has been found in the study between stomata openings and soybean yields.

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