Stomata dan Trikoma Kultivar Kedelai Anjasmoro selama pemupukan Nanosilika dan Plant Growth Promoting Rhizobacteria

Soybean is a very important crop commodity in Indonesia. Increasing soybean production on dryland as cultivation land need to be carried out. Dryland have issues with either of nutrient and water availability which is not supportable for plant growth. Therefore, the application of nano-silica and plant growth promoting rhizobacteria (PGPR) are expected to increase nutrient absorption, synthesis of plant growth hormone and increase in plant resistance to drought stress. The growth of drought-resistant plants is able to be showed through the status of stomata and trichomes on leaves. So that, this study aimed to determine the stomata and trichome performance of soybean plants of Anjasmoro cultivar using nano-silica and PGPR as fertilizers. The study design used a completely randomized design with 2 factors with three replication. The first factor was nano silica concentration i.e. 0, 100 and 200 ppm. The second factor was PGPR concentration i.e. 0, 5, 10, and 15%. Results showed that the nanosilica and PGPR applications had no significant effect on stomata and trichome status of Anjasmoro cultivar except for stomatal aperture and the ratio of the size of the stomata openings. A positive correlation has been found in the study between stomata openings and soybean yields.

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Effectiveness of Mycorrhiza, Plant Growth Promoting Rhizobacteria and Inorganic Fertilizer on Chlorophyll Content in Glycine max (L.) cv. Detam-4 Prida

E3S Web of Conferences ◽

10.1051/e3sconf/202122600031 ◽

2021 ◽

Vol 226 ◽

pp. 00031

Author(s):

Muhammad Muhammad ◽

Umi Isnatin ◽

Peeyush Soni ◽

Praptiningsih Gamawati Adinurani

Keyword(s):

Plant Growth ◽

Chlorophyll Content ◽

Plant Growth Promoting Rhizobacteria ◽

Nitrogen And Phosphorus ◽

Randomized Design ◽

Effective Combination ◽

Plant Growth Promoting ◽

Glycine Max L ◽

Growth Promoting ◽

Completely Randomized Design

This study aimed to find an effective combination of mycorrhiza, PGPR (Plant Growth Promoting Rhizobacteria), and inorganic fertilizers. Whereas the specific purpose was observed in effective mycorrhiza to increased chlorophyll content. This study used a completely randomized design (CRD) with three factors: the 1st factor is mycorrhiza application, the 2nd factor is PGPR and the 3rd factor is the application of nitrogen and phosphorus fertilizers. The data were analyzed with Analysis of Variance to determine the effect of the treatment being tried. Continued with the Least Significance Different test at a 95 % confidence level. The results indicated that the most effective application in increasing total chlorophyll content was (i) the ‟Commercial Mycorrhiza” without being combined with PGPR and fertilizer (TP.TR.MP:80 g mL–1). (ii) ‟Brawijaya Mycorrhiza” is combined with PGPR without fertilizer (TP.R.MB: 83 g mL–1). (iii) ‟Unida Mycorrhiza” without combined with PGPR and without fertilizer (TP.TR.MU: 80 g mL–1).

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Comparative Effects of Bio-Wastes in Combination with Plant Growth-Promoting Bacteria on Growth and Productivity of Okra

Agronomy ◽

10.3390/agronomy11102065 ◽

2021 ◽

Vol 11 (10) ◽

pp. 2065

Author(s):

Hammad Anwar ◽

Xiukang Wang ◽

Azhar Hussain ◽

Muhammad Rafay ◽

Maqshoof Ahmad ◽

...

Keyword(s):

Plant Growth ◽

Soil Health ◽

Organic Amendments ◽

Plant Growth Promoting Rhizobacteria ◽

Fruit Weight ◽

Growth And Yield ◽

Plant Growth Promoting Bacteria ◽

Randomized Design ◽

Plant Growth Promoting ◽

Growth Promoting

Plant growth-promoting rhizobacteria with multiple growth-promoting traits play a significant role in soil to improve soil health, crop growth and yield. Recent research studies have focused on the integration of organic amendments with plant growth-promoting rhizobacteria (PGPR) to enhance soil fertility and reduce the hazardous effects of chemical fertilizers. This study aims to evaluate the integrated application of biochar, compost, fruit and vegetable waste, and Bacillus subtilis (SMBL 1) to soil in sole application and in combined form. The study comprises eight treatments—four treatments without inoculation and four treatments with SMBL 1 inoculation in a completely randomized design (CRD), under factorial settings with four replications. The results indicate that the integrated treatments significantly improved okra growth and yield compared with sole applications. The integration of SMBL 1 with biochar showed significant improvements in plant height, root length, leaf chlorophyll a and b, leaf relative water content, fruit weight, diameter and length by 29, 29, 50, 53.3, 4.3, 44.7 and 40.4%, respectively, compared with control. Similarly, fruit N, P and K contents were improved by 33, 52.7 and 25.6% and Fe and Zn in shoot were 37.1 and 35.6%, respectively, compared with control. The results of this study reveal that the integration of SMBL 1 with organic amendments is an effective approach to the sustainable production of okra.

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APLIKASI RHIZOBAKTERI PEMACU TUMBUH (RPT) DARI AKAR TITONIA (Tithonia diversifolia) TERHADAP PERTUMBUHAN STEK MELATI (Jasminum officinale) PADA ULTISOL

Jurnal Solum ◽

10.25077/jsolum.15.2.75-82.2018 ◽

2018 ◽

Vol 15 (2) ◽

pp. 75

Author(s):

Agustian Agustian ◽

Anisah Permata Sari ◽

Lusi Maira

Keyword(s):

Plant Height ◽

Plant Growth Promoting Rhizobacteria ◽

Tithonia Diversifolia ◽

Branch Number ◽

Randomized Design ◽

Plant Growth Promoting ◽

Number Of Leaves ◽

Growth Promoting ◽

Completely Randomized Design

Jasmine plant is a horticultural crop that has promising prospect to be developed in trading. The development of jasmine cultivation is determined by the quality of seedling that can be produced rapidly for planting in the field. The purpose of this research is to study the effect of application of Plant growth-promoting rhizobacteria (PGPR) from titonia root (Tithonia diversifolia) in increasing the growth of jasmine cuttings (Jasminum officinale). This experiment used Completely Randomized Design (RAL) which consists of 7 treatments with 5 replications. The treatments were: A (control), B (Growtone), C (Growtone + 1 ml PGPR), D (Growtone + 2 ml PGPR), E (Growtone + 4 ml PGPR) , F (Growtone + 6 ml PGPR) and G (Growtone + 8 ml PGPR). Data obtained were analyzed statistically with F test at 5% level. The results showed that the application of PGPR can increase the growth of jasmine cuttings. However, the increase in PGPR dose did not have a significant effect on the increase of leaf number, branch number and height of jasmine cuttings. The highest number of leaves, branch and plant height at two months seedling age were found in treatment (C) 1 ml PGPR with 48.60 leaves, 5.80 branch and 22.90 cm plant height that significantly different from other treatments,. Treatment (D) 2 ml PGPR application increased the population of P solubilizing bacteria in rhizosphere from 2.59x104 cfu/g of soil at control to 5.05x105 cfu/g soil and fixing bacteria N (Azospirillium) 3x104 cfu/g soil to 1.41x105 cfu/g soil and Azotobacter to 1.2x 103 cfu/g soil.Key words : Jasmine, PGPR, cuttings, Ultisol

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COMPATIBILITY OF AZOXYSTROBIN AND CYPROCONAZOLE ON PLANT GROWTH-PROMOTING MICROORGANISMS

JOURNAL OF NEOTROPICAL AGRICULTURE ◽

10.32404/rean.v7i4.5281 ◽

2020 ◽

Vol 7 (4) ◽

pp. 95-100

Author(s):

Weslany Silva Rocha ◽

Mara Caroline Alves da Silva ◽

Gilberto Coutinho Machado Filho ◽

Mauro Gomes Dos Santos ◽

Aloísio Freitas Chagas Júnior ◽

...

Keyword(s):

Plant Growth ◽

Bradyrhizobium Japonicum ◽

Integrated Management ◽

Growth Promoter ◽

Randomized Design ◽

Plant Growth Promoting ◽

Agroecosystem Management ◽

Growth Promoting ◽

Completely Randomized Design ◽

Set Up

Avoiding the toxicity effect of chemical fungicides on rhizobacteria is a sustainable alternative for agroecosystem management. Rhizobacteria, whose bioprotective and plant growth-promoter potential have been reported in the literature, lack studies on their performance in integrated management with pesticides. Thus, this study aimed to evaluate the effects of azoxystrobin + cyproconazole on the growth of Bacillus subtilis, Streptomyces seoulensis, and Bradyrhizobium japonicum. Three independent experiments were set up, i.e., one for each microorganism, and carried out in a completely randomized design in a factorial scheme (3 × 6), with three doses (recommended by the manufacturer, half, and twice) and six periods of evaluations (48, 96, 144, 192, 240, and 288 hours), with three replications. Growth inhibition rings were evaluated. Azoxystrobin + cyproconazole at all tested doses is compatible with B. subtilis. The use of azoxystrobin + cyproconazole affected the growth of B. japonicum and S. seoulensis, which were sensitive to all its doses until 288 hours after inoculation.

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Plant Growth-Promoting Rhizobacteria: Effects on Yield and Nutritive Value of ‘Russell’ Bermudagrass and ‘KY 31’ Tall Fescue

Journal of Animal Science ◽

10.1093/jas/skab096.036 ◽

2021 ◽

Vol 99 (Supplement_2) ◽

pp. 21-21

Author(s):

Madison Cole ◽

S Leanne L Dillard

Keyword(s):

Plant Growth ◽

Tall Fescue ◽

Nutritive Value ◽

Plant Growth Promoting Rhizobacteria ◽

Single Strain ◽

Randomized Design ◽

Plant Growth Promoting ◽

Nirs Data ◽

Growth Promoting ◽

N Fertility

Abstract Commercial N fertilizer are labor intensive and expensive for many forage producers. Alternative N fertility options are necessary for the long-term sustainability of forage systems. A 2-yr experiment evaluating plant growth-promoting rhizobacteria (PGPR) as an alternative N source for ‘Russell’ bermudagrass (C. dactylon) and ‘KY 31’ tall fescue (L. arundinaceum) was conducted in Alabama. Fourteen, 3-m2 plots were treated with full N (56 kg/ha) and ½ N (28 kg/ha), Accomplish LM (AMS), AMS + ½ N, DH44, Blend 20, and a control. DH44 is a single strain of Paenibacillus sonchi, while Blend 20 contains 2 strains of Bacillus pumilus and 1 strain of B. spaericus. Forage samples were taken every 4 weeks with a 0.1-m2 quadrat then analyzed for NDF, ADF, CP, and yield using NIRS. Data were analyzed using Proc GLIMMIX of SAS 9.4 (SAS Inst., Cary, NC) as a completely randomized design (n = 2). For both forages, full N had greater (P < 0.0139) yield when compared to the control (tall fescue: 8,295 vs. 7,353 kg/ha; bermudagrass: 9,329 vs. 8,109 kg/ha). There were no differences (P ≥ 0.05) in NDF concentration for either forage. Full and ½ N had greater CP than AMS in bermudagrass (11.5, 11.4%; P ≤ 0.0490). Blend 20 treated bermudagrass had greater ADF than full and ½ N (32.7, 30.7, and 30.9%, respectively; P < 0.0313). Blend 20 treated tall fescue had greater ADF compared to AMS + N and DH44 (35.8, 12.1, and 33.9%, respectively; P < 0.0227). Full N tall fescue had greater CP) compared to AMS (12.1, AMS CP %, respectively; P < 0.0082). DH44 had greater CP compared to AMS and Blend 20 (11.9, 11.0, and 11.0%, respectively; P < 0.0423). For both forages, PGPR treated plots produced yields and maintained forage nutritive value not different than the commercial fertilizer.

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Isoflavone content and antioxidant activity of soybean inoculated with plant-growth promoting rhizobacteria

Journal of the Serbian Chemical Society ◽

10.2298/jsc160422070k ◽

2016 ◽

Vol 81 (11) ◽

pp. 1239-1249 ◽

Cited By ~ 5

Author(s):

Biljana Kiprovski ◽

Djordje Malencic ◽

Simonida Djuric ◽

Mira Bursac ◽

Jelena Cvejic ◽

...

Keyword(s):

Antioxidant Activity ◽

Plant Growth ◽

Positive Impact ◽

Plant Growth Promoting Rhizobacteria ◽

Phenylpropanoid Pathway ◽

Soybean Seedlings ◽

Isoflavone Content ◽

Plant Growth Promoting ◽

Growth Promoting ◽

Soybean Plants

Plant-growth promoting rhizobacteria (PGPR) elicit activation of phenylpropanoid pathway in plants which leads to phenolics production and enhanced antioxidant capacity. The purpose of this work was to assess the antioxidant activity of soybean plants, Glycine max L., inoculated with PGPR (isolates of Azotobacter chroococcum, Streptomyces sp. and mixture of these) during plant development, as well as yield of inoculated soybean plants. PGPR applied in the experiment stimulated flavonoids and isoflavone synthesis, which enhanced non-enzymatic antioxidant ability of soybean plants. Also, PGPRs stimulated accumulation of daidzein and genistin in soybean seedlings (5-fold and 2-fold compared to control values, respectively). The mixture of PGPRs showed positive impact on antioxidant activity (10-20% higher activity) and yield components of soybean which proposed this inoculum as possibly potent bio-fertilizer in soybean production.

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Effect of plant growth promoting rhizobacteria (PGPR) and water stress on phytohormones and polyamines of soybean

Indian Journal of Agricultural Research ◽

10.18805/ijare.v49i5.5805 ◽

2015 ◽

Vol 49 (5) ◽

Cited By ~ 3

Author(s):

Hossein Zahedi ◽

Samira Abbasi

Keyword(s):

Abscisic Acid ◽

Drought Stress ◽

Plant Growth ◽

Plant Growth Promoting Rhizobacteria ◽

Control Treatment ◽

Rhizobium Japonicum ◽

Severe Drought ◽

Plant Growth Promoting ◽

Growth Promoting ◽

Soybean Plants

The effect of inoculation of three plant growth promoting rhizobacteria (PGPR) that is <italic>Rhizobium japonicum</italic>, <italic>Azotobacter chroococcum</italic> and <italic>Azospirillum brasilense</italic> and mixture of them on phytohormones and polyamines of soybean under different irrigation regimes was investigated. Drought stress induced by irrigation withholding until 40, 80 and 120 mm evaporation from evaporation pan. However seed bacterization of soybean was accompanied with 20 kg ha−1 nitrogen. In addition, 20 and 100 kg ha−1 nitrogen were considered as control treatments. The results showed that drought stress significantly decreased cytokinin, gibberellin and auxin accumulation in plant tissues. By contrast, drought stress led to increase in abscisic acid accumulation in soybean plants. Polyamines that are putrescine and spermidine increased due to drought stress and then decreased under severe drought stress. PGPR application had positive effect on growth promoting phytohormones compared to control treatment. However the highest accumulation of cytokinin, gibberellin and auxin was related to 100 kg ha−1 nitrogen treatment. In case of abscisic acid PGPR application decreased its accumulation. Asignificant decrease as observed on polyamines accumulation when PGPRs were applied on stressed soybean plants.

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Various Shallot Seed Treatments with Trichoderma to Increase Growth and Yield on Sandy Coastal

Ilmu Pertanian (Agricultural Science) ◽

10.22146/ipas.12564 ◽

2017 ◽

Vol 1 (3) ◽

pp. 094 ◽

Cited By ~ 1

Author(s):

Stefany Darsan ◽

Endang Sulistyaningsih ◽

Arif Wibowo

Keyword(s):

Plant Growth ◽

Seed Treatment ◽

Growth And Yield ◽

Area Index ◽

Randomized Design ◽

Plant Growth Promoting ◽

Growth Promoting ◽

Completely Randomized Design ◽

Net Assimilation ◽

Coastal Land

Sandy coastal area is a poor land, with minimal nutrients and low ability to store water and nutrition. Therefore, plant is difficult to grow well, consequently the treatments for improving sandy coastal is required before planting to support their growth. Application of Trichoderma as a plant-growth promoting and controlling pathogens had been known. Shallot seed treatment by Trichoderma agents was intended to improve and support plant growth and yield in the sandy coastal land. The aim of experiment was to determine the most effective treatment to improve growth and yield of shallot on sandy coastal land. The experiment had been carried out in Yogyakarta during August - November 2015. The factorial treatments of cultivar and Trichoderma applications were arranged in Completely Randomized Design with three replications. The shallot cultivars consisted of Tiron, Crok, and Biru, while Trichoderma application consisted of control (no treatment), sprayed with Trichoderma, soaked in Trichoderma, matricontioning media i.e. rice husk charcoal and brick in combination with Trichoderma. The collected data related to plant growth and yield. The result showed that seed treatment methods with Trichoderma increased the plant height, the leaf area index, extensive root, net assimilation rate (NAR), plant growth rate (PGR), Nitrate Reductase Activities (NRA), total chlorophyll, and fresh bulb weight. The best technical treatment for cultivar Tiron was soaked with Trichoderma, and Crok was sprayed with Trichoderma, while Biru cultivar was using brick in combination with Trichoderma.

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