scholarly journals The Occurrence and Comparative Phenotypic Characteristics of Staphylococcus spp. from Healthy and Diseased, Household and Shelter Dogs, Based on Routine Biochemical Diagnostic Methods

2011 ◽  
Vol 60 (1) ◽  
pp. 19-26 ◽  
Author(s):  
ANDRZEJ KASPROWICZ ◽  
ANNA BIAŁECKA ◽  
JOANNA BIAŁECKA ◽  
IZABELA GODZISZ ◽  
WIESŁAW BARABASZ ◽  
...  
Keyword(s):  
Skin Lesions ◽  
Common Species ◽  
Carrier State ◽  
Diagnostic Methods ◽  
Animal Shelter ◽  
Staphylococcal Species ◽  
Staphylococcus Intermedius ◽  
Shelter Dogs ◽  
Staphylococcus Spp ◽  
Infected Skin

To determine the staphylococcal colonization pattern in healthy and diseased dogs, living in two particular environments, a number of microbiological samples were taken. Overall, twenty dogs, either healthy or with infected skin lesions, were examined. In each case bacterial swabs were collected from the nasal mucosa, ear, perineum, lumbo-sacralis triangle, and from the infection sites if such were present. A total number of 104 isolates representing different staphylococcal species were isolated and identified using routine biochemical methods applied in diagnostic laboratories. Among 17 isolated staphylococcal species, Staphylococcus intermedius was the most common species isolated from both healthy or diseased dogs living either in animal shelter or household environments. The pattern of Staphylococcus sp. colonization differs considerably for animals living in the two tested habitats. In particular, S. aureus MRSA and MSSA isolates were detected only in infected skin lesion samples from animals that dwelled in the animal shelter. As could be expected, S. intermedius was found to be a predominant causative agent in canine skin infections. In our study, we demonstrated that S. intermedius in its carrier-state, inhabits mainly the mucosal membrane of the nasal vestibule. It was also found in the samples taken from the skin, the lumbo-sacralis triangle and perineum, but was rarely isolated from the ears.

scholarly journals In vitro antibiotic resistance of Staphylococci isolated from different animal species

2020 ◽  
Vol 44 (5) ◽  
pp. 1055-1062
Author(s):  
Nurdan KARACAN SEVER ◽  
Mehmet AKAN
Keyword(s):  
Antibiotic Resistance ◽  
Multidrug Resistance ◽  
Animal Species ◽  
Common Species ◽  
Oxacillin Resistance ◽  
Staphylococcus Intermedius ◽  
Rational Use Of Antibiotics ◽  
Resistance Rates ◽  
Staphylococcus Spp

The purpose of this study is to investigate resistance to antibiotics of Staphylococcus species isolated from various samples belonging to different animal species. Among 48 Staphylococcus spp. strains, Staphylococcus intermedius was the most common species, followed by S. aureus, S. epidermidis, S. hyicus, S. saprophyticus. In a total of 48 Staphylococcus strains, the highest antibiotic resistance was observed to oxacillin (79.17%), tetracycline (39.58%), and ampicillin and cefoxitin (31.25%). Of 48 Staphylococcus strains, 42 showed resistance to at least one antimicrobial agent, while 23 of the strains had multidrug resistance. Antimicrobial resistance to tetracycline and ampicillin, erythromycin, streptomycin was detected frequently in S. aureus,S. intermedius, and S. epidermidis, respectively. Resistance rates for ampicillin, cefoxitin, and enrofloxacin were determined as 66.67% in S. hyicus strains. S. saprophyticus was determined to show resistance to 13 antibiotics other than meropenem. The highest antibiotic resistance was determined in S. aureus, S. intermedius, S. epidermidis, and in 48 Staphylococcus strains to oxacillin. Consequently, this study revealed resistance to various antibiotics in Staphylococcus species. Additionally, the presence of high oxacillin resistance and multidrug resistance in the Staphylococcus strains revealed the importance of determination of antimicrobial susceptibility before treatment and for rational use of antibiotics.

Unusual cryptosporidiosis cases in Swedish patients: extended molecular characterization ofCryptosporidium viatorumandCryptosporidiumchipmunk genotype I

Parasitology ◽  
2013 ◽  
Vol 140 (14) ◽  
pp. 1735-1740 ◽  
Author(s):  
MARIANNE LEBBAD ◽  
JESSICA BESER ◽  
MONA INSULANDER ◽  
LILLEMOR KARLSSON ◽  
JENS G. MATTSSON ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Cryptosporidium Parvum ◽  
Geographical Variation ◽  
Indian Subcontinent ◽  
Common Species ◽  
Diagnostic Methods ◽  
Molecular Diagnostic ◽  
Cryptosporidium Hominis ◽  
Genotype I ◽  
Protein Locus

SUMMARYMost human cases of cryptosporidiosis are caused byCryptosporidium parvumorCryptosporidium hominis, but the use of molecular diagnostic methods has revealed that several other less common species or genotypes can also be involved. Here, we describe two unusual causes of cryptosporidiosis, one being the recently described speciesCryptosporidium viatorumand the otherCryptosporidiumchipmunk genotype I. Two Swedish patients who were infected withC. viatorumhad travelled to Kenya and Guatemala, respectively, and two others had been infected withCryptosporidiumchipmunk genotype I in Sweden. None of these four patients were immunocompromised, and all four showed classical symptoms of cryptosporidiosis. We performed extensive molecular characterization, including analysis of four loci. The twoC. viatorumisolates were found to differ slightly at the 70-kDa heat shock protein locus, which may indicate a local geographical variation in this species that has previously been described exclusively on the Indian subcontinent.

Effects of low intensity laser irradiation during healing of infected skin lesions in the rat

2006 ◽  
Author(s):  
Ethne L. Nussbaum ◽  
Lothar Lilge ◽  
Tony Mazzulli ◽  
Kenneth P. Pritzker
Keyword(s):  
Laser Irradiation ◽  
Skin Lesions ◽  
Low Intensity ◽  
Intensity Laser ◽  
Infected Skin

Nannizzia incurvata. [Descriptions of Fungi and Bacteria].

1965 ◽  
Author(s):  
P. M. Stockdale
Keyword(s):  
Great Britain ◽  
Geographical Distribution ◽  
Guinea Pigs ◽  
Tinea Capitis ◽  
World Wide ◽  
Skin Lesions ◽  
Wide Distribution ◽  
Tinea Corporis ◽  
Soil P ◽  
Infected Skin

Abstract A description is provided for Nannizzia incurvata. Information is included on the disease caused by the organism, its transmission, geographical distribution, and hosts. HOSTS: Recorded only from man and dog (but see NOTES). Guinea-pigs have been experimentally infected. DISEASE: Ringworm (dermatophytosis, tinea). Nannizzia incurvata is present in soil and apparently only rarely a cause of disease. In man the scalp (tinea capitis) and glabrous skin (tinea corporis) may be infected. Skin lesions are inflammatory but details of known scalp infections are not available. In experimental inoculations of guineapigs (Rdzanek, pers. comm.) N. incurvata was intermediate between N. gypsea and N. fulva in virulence, the reaction varying from negative to strongly inflammatory. Ectothrix hyphae breaking up into large arthrospores were seen on some hairs, and infected hairs did not fluoresce under Wood's light. GEOGRAPHICAL DISTRIBUTION: Asia (India), Europe (Czechoslovakia, Great Britain and Ireland, Germany, Netherlands, Poland); U.S.A. (Tenn.); N. incurvata is probably of world-wide distribution in the soil.

Lipid-Based Nanocarriers for the Treatment of Infected Skin Lesions

2017 ◽  
pp. 385-430
Author(s):  
Sandra Simöes ◽  
Manuela Carvalheiro ◽  
Maria Manuela Gaspar
Keyword(s):  
Skin Lesions ◽  
Infected Skin

Application of a welfare assessment tool (Shelter Quality Protocol) in 64 Italian long-term dogs' shelters: welfare hazard analysis

2019 ◽  
Vol 28 (3) ◽  
pp. 353-363
Author(s):  
L Arena ◽  
GV Berteselli ◽  
F Lombardo ◽  
L Candeloro ◽  
P Dalla Villa ◽  
...  
Keyword(s):  
Assessment Tool ◽  
Body Condition Score ◽  
Descriptive Analysis ◽  
Weather Conditions ◽  
Skin Lesions ◽  
Feeding Regime ◽  
Essential Components ◽  
Adverse Weather ◽  
Shelter Dogs

The confined environment of the dog shelter, particularly over extensive time-periods can impact severely on welfare. Surveillance and assessment are therefore essential components of the welfare protocol. The aim of this study was to generate a descriptive analysis of a sample of Italian long-term shelters and identify potential hazards regarding the welfare of shelter dogs. This was achieved through application of the Shelter Quality Protocol (SQP) to link income/outcome variables and the inclusion of sixty-four long-term shelters in Italy. Descriptive and logistic regression analyses were conducted. Key findings showed feeding regime, type of diet and access to outdoor area to be significantly associated with inadequate body condition score (BCS). The probability of observing skin lesions was shown to be influenced by bedding inadequacy and bedding type. Limiting beds to one per dog and utilising clean bedding materials was significantly associated with a reduced probability of observing dirty/wet dogs. Protection from adverse weather conditions and inadequate bedding were significantly associated with the manifestation of polypnea. Non-existent dog training facilities, outdoor access or leash walking were all found to significantly increase the likelihood of fearful or aggressive attitudes to people. Outdoor access also, in conjunction with feeding regime, was associated with the presence of diarrhoea. The SQP proved useful in identifying welfare hazards, both as regards shelter environment and shelter management. Identification of these hazards creates the opportunity for interventions to be applied, minimising the risks and improving the welfare of long-term shelter dogs.

scholarly journals CARACTERIZAÇÃO E PERFIL DE SENSIBILIDADE DE STAPHYLOCOCCUS SPP. ISOLADOS DE AMOSTRAS DE CARNE CAPRINA COMERCIALIZADAS EM MERCADOS E SUPERMERCADOS EM RECIFE, PE

2006 ◽  
Vol 73 (1) ◽  
pp. 7-15
Author(s):  
A.P.B.L. Moura ◽  
R. Acioli ◽  
D.A.M. Duarte ◽  
J.W. Pinheiro Junior ◽  
J.S. Alcântara ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Staphylococcus Intermedius ◽  
Staphylococcus Spp

RESUMO Objetivou-se com este trabalho isolar, identificar, quantificar e avaliar o perfil de sensibilidade antimicrobiana in vitrode amostras de Staphylococcus spp. procedentes de 24 amostras de carne caprina in natura e resfriada, comercializadas em mercados públicos/privados e supermercados da Cidade do Recife. Foram utilizadas metodologias oficiais preconizadas pelo Ministério da Agricultura Pecuária e Abastecimento (MAPA). Os resultados demonstraram que 11 (45,83%) amostras apresentaram contagens de estafilococos coagulase positiva (SCP) variando de 6,7 x 103 a 1,4 x 106 UFC/g correspondendo a 27 isolados de um total de 89, destes 19,10% foram classificadas como Staphylococcus aureus, 8,99% como Staphylococcus hyicus e 2,25 como Staphylococcus intermedius. Na avaliação do perfil de sensibilidade, os antibióticos mais eficazes foram norfloxacina e vancomicina (100%), tetraciclina e sulfa + trimetoprim (96,30%) e oxacilina (87,50%). Amoxicilina (59,26%), penicilina (70,37%) e novobiocina (55,55%) mostraram diferentes percentuais de resistência frente às amostras. Conclui-se que a carne caprina apresenta elevada carga microbiana, com relação a contagem de Staphylococcus spp., demonstrando a necessidade de rever a resolução que regulamenta os padrões micróbio lógicos para carnes de caprinos in natura e resfriadas.

scholarly journals IDENTIFICATION OF MALASSEZIA SPECIES AND ITS ASSOCIATION WITH CLINICAL MANIFESTATIONS OF ATOPIC DERMATITIS

2021 ◽  
Vol 506 (1-2) ◽  
Author(s):  
Tran Cam Van ◽  
Nguyen Van Hieu
Keyword(s):  
Atopic Dermatitis ◽  
Clinical Manifestations ◽  
Morphological Characteristics ◽  
Skin Lesions ◽  
Common Species ◽  
Atopic Dermatitis Patient ◽  
Inflammatory Skin Disease ◽  
Direct Examination ◽  
National Hospital ◽  
Malassezia Species

Introduction: Atopic dermatitis is a chronic, recurrent inflammatory skin disease that is characterized by an eczematous reaction. Few studies have investigated fungi in the pathogenesis of atopic dermatitis, however, there are different about distribution of Malassezia species. Objectives: To indentificate of Malassezia species and its asociation with clinical manifestations in Vietnamese atopic dermatitis patient. Methods: 178 patients who were diagnosed with atopic dermatitis and had a postitive direct examination of Malassezia at the National hospital of dermatology and venereology between July 2019 and June 2020.  Specimens were taken with cellotape, then stained in 20% of potassium hydroxit combined with ParkerTM blue black ink.  All patient who had postive test were cultured on SDA and mDixon. For fungal samples, we selected pure colonies with morphological characteristics of yeast as follows about 1cm in diameter, round, cream or milky in color, smooth and glossy to detect the species. Results: From the samples of atopic dermatitis patients, we cultured and idenfified 41 cases. 5 species were found, in which M. globosa was the most common species, accounting for 39%, followed by M. restricta (19.5%), M. dermatis (17.1%),  M. furfur (17.1%) and M. sympodialis (2.4%). Conclusion: On the skin lesions of Vietnamese patients with atopic dermatitis, M. globosa was the most common species with 39.0%.

Nannizzia fulva. [Descriptions of Fungi and Bacteria].

1965 ◽  
Author(s):  
P. M. Stockdale
Keyword(s):  
Great Britain ◽  
South America ◽  
Geographical Distribution ◽  
Inflammatory Reaction ◽  
Tinea Capitis ◽  
World Wide ◽  
Skin Lesions ◽  
Limiting Factors ◽  
Proved Case ◽  
Infected Skin

Abstract A description is provided for Nannizzia fulva. Information is included on the disease caused by the organism, its transmission, geographical distribution, and hosts. HOSTS: Recorded only from man (but see NOTES). Guinea-pigs have been experimentally infected. DISEASE: Ringworm (dermatophytosis, tinea). Nannizzia fulva is present in the soil and apparently only rarely pathogenic. The scalp (tinea capitis) and glabrous skin (tinea corporis) may be infected. Skin lesions are inflammatory. The only proved case of scalp infection is that recorded by Uriburu (1909); according to Sabouraud (Les Teignes, 1910, p. 241) in this case there was a light inflammatory reaction and infected hairs were indistinguishable from those in M. audouinii infections. In experimental inoculations of guineapigs (Rdzanek, pers. comm.) N. fulva was slightly less virulent than N. gypsea and N. incurvata, the reaction varying from negative to strongly inflammatory. A few ectothrix hyphae breaking up into arthrospores were seen on some hairs, and infected hairs did not fluoresce under Wood's light. GEOGRAPHICAL DISTRIBUTION: Africa (Tanzania), Europe (Great Britain, Hungary, Yugoslavia), South America (Argentina). Probably world-wide in distribution in the soil, but there may be local limiting factors (e.g., in Great Britain it has been recorded only for Bristol, Somerset, and Wiltshire).

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