scholarly journals IDENTIFICATION OF MALASSEZIA SPECIES AND ITS ASSOCIATION WITH CLINICAL MANIFESTATIONS OF ATOPIC DERMATITIS

2021 ◽  
Vol 506 (1-2) ◽  
Author(s):  
Tran Cam Van ◽  
Nguyen Van Hieu
Keyword(s):  
Atopic Dermatitis ◽  
Clinical Manifestations ◽  
Morphological Characteristics ◽  
Skin Lesions ◽  
Common Species ◽  
Atopic Dermatitis Patient ◽  
Inflammatory Skin Disease ◽  
Direct Examination ◽  
National Hospital ◽  
Malassezia Species

Introduction: Atopic dermatitis is a chronic, recurrent inflammatory skin disease that is characterized by an eczematous reaction. Few studies have investigated fungi in the pathogenesis of atopic dermatitis, however, there are different about distribution of Malassezia species. Objectives: To indentificate of Malassezia species and its asociation with clinical manifestations in Vietnamese atopic dermatitis patient. Methods: 178 patients who were diagnosed with atopic dermatitis and had a postitive direct examination of Malassezia at the National hospital of dermatology and venereology between July 2019 and June 2020.  Specimens were taken with cellotape, then stained in 20% of potassium hydroxit combined with ParkerTM blue black ink.  All patient who had postive test were cultured on SDA and mDixon. For fungal samples, we selected pure colonies with morphological characteristics of yeast as follows about 1cm in diameter, round, cream or milky in color, smooth and glossy to detect the species. Results: From the samples of atopic dermatitis patients, we cultured and idenfified 41 cases. 5 species were found, in which M. globosa was the most common species, accounting for 39%, followed by M. restricta (19.5%), M. dermatis (17.1%),  M. furfur (17.1%) and M. sympodialis (2.4%). Conclusion: On the skin lesions of Vietnamese patients with atopic dermatitis, M. globosa was the most common species with 39.0%.

scholarly journals Inhibitory Effect ofNelumbo nucifera(Gaertn.) on the Development of Atopic Dermatitis-Like Skin Lesions in NC/Nga Mice

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Rajendra Karki ◽  
Myung-A Jung ◽  
Keuk-Jun Kim ◽  
Dong-Wook Kim
Keyword(s):  
Atopic Dermatitis ◽  
Severity Score ◽  
Histopathological Examination ◽  
Skin Lesions ◽  
Transepidermal Water Loss ◽  
Inhibitory Effect ◽  
Clinical Severity ◽  
Inflammatory Skin Disease ◽  
Level Measurement ◽  
Different Doses

Atopic dermatitis (AD) is a chronic inflammatory skin disease which has a complex etiology that encompasses immunologic responses. The study was carried out to examine the effect ofNelumbo nucifera(Gaertn.) leaf (NL) on the AD-like skin lesion induced by repeated epicutaneous application of 2,4-dinitrochlorobenzene (DNCB) on the dorsal skin of NC/Nga mice. Three different doses of NL (5, 25, and 50 mg/mice/day) were administered orally from the day of sensitization with DNCB for 4 weeks. The efficacy of NL was judged by histopathological examination, blood IgE level, measurement of transepidermal water loss (TEWL), scratching behavior, and skin severity score. NL resulted in the suppression of clinical severity score, TEWL, scratching behavior, and blood IgE level. Histopathologic analyses revealed that thickening of the epidermis and mast cell degranulation was significantly reduced in NL group. These results suggest that NL may be a useful natural resource for the management of AD.

Malassezia species dysbiosis in natural and allergen-induced atopic dermatitis in dogs

2019 ◽  
Vol 58 (6) ◽  
pp. 756-765 ◽  
Author(s):  
Courtney Meason-Smith ◽  
Thierry Olivry ◽  
Sara D Lawhon ◽  
Aline Rodrigues Hoffmann
Keyword(s):  
Atopic Dermatitis ◽  
Its Region ◽  
Skin Lesions ◽  
Cost Of Care ◽  
Skin Lipid ◽  
Malassezia Species ◽  
Allergic Skin ◽  
Culture Independent ◽  
Atopic Skin ◽  
Species Specific

Abstract Malassezia dermatitis and otitis are recurrent features of canine atopic dermatitis, increasing the cost of care, and contributing to a reduced quality of life for the pet. The exact pathogenesis of secondary yeast infections in allergic dogs remains unclear, but some have proposed an overgrowth of M. pachydermatis to be one of the flare factors. The distribution of Malassezia populations on healthy and allergic canine skin has not been previously investigated using culture-independent methods. Skin swabs were collected from healthy, naturally affected allergic, and experimentally sensitized atopic dogs. From the extracted DNA, fungal next-generations sequencing (NGS) targeting the ITS region with phylogenetic analysis of sequences for species level classification, and Malassezia species-specific quantitative real-time polymerase chain reaction (qPCR) were performed. M. globosa was significantly more abundant on healthy canine skin by both methods (NGS P < .0001, qPCR P < .0001). M. restricta was significantly more abundant on healthy skin by NGS (P = .0023), and M. pachydermatis was significantly more abundant on naturally-affected allergic skin by NGS (P < .0001) and on allergen-induced atopic skin lesions by qPCR (P = .0015). Shifts in Malassezia populations were not observed in correlation with the development of allergen-induced skin lesions. Differences in the lipid dependency of predominant Malassezia commensals between groups suggests a role of the skin lipid content in driving community composition and raises questions of whether targeting skin lipids with therapeutics could promote healthy Malassezia populations on canine skin.

scholarly journals Occurrence of Malassezia Yeasts in Dermatologically Diseased Dogs

2017 ◽  
Vol 61 (2) ◽  
pp. 17-21
Author(s):  
Z. Sihelská ◽  
E. Čonková ◽  
P. Váczi ◽  
M. Harčárová ◽  
E. Böhmová
Keyword(s):  
Otitis Externa ◽  
Clinical Manifestations ◽  
Morphological Characteristics ◽  
Skin Lesions ◽  
Pcr Rflp ◽  
Malassezia Spp ◽  
Interdigital Dermatitis ◽  
Lipophilic Yeasts ◽  
Anal Sacs ◽  
Isolated Species

AbstractThe Malassezia genus is represented by several lipophilic yeasts, normally present on the skin of many warm-blooded vertebrates, including humans. The aim of this study was to investigate the occurrence of Malassezia yeasts in dogs with skin lesions (dermatitis, interdigital dermatitis and inflammation of anal sacs) and otitis externa. The presence of Malassezia spp. was investigated in a group of 300 dogs exhibiting clinical manifestations. The isolates of Malassezia were identified by using phenotypic (biochemical-physiological and morphological characteristics) and genotypic methods (PCR, RFLP-AluI, BanI and MspA1I) which allowed their precise identification. Malassezia yeasts were isolated from 84 specimens obtained from 76 positive dogs. M. pachydermatis was the most frequently isolated species (79 isolates) in this study. M. furfur was identified in four dogs and M. nana in one dog. The prevalence of isolated Malassezia spp. was 25.3 % in dogs with skin lesions; from which 36.0 % were dogs suffering from otitis externa, 24.5 % from dogs having dermatitis, 16.4 % from dogs with interdigital dermatitis and 14.3 % from dogs having inflammation of the anal sacs. A higher prevalence of Malassezia spp. was observed in animals with pendulous ears in comparison with dogs having erect ears.

scholarly journals Effects of Feeding a Hypoallergenic Diet with a Nutraceutical on Fecal Dysbiosis Index and Clinical Manifestations of Canine Atopic Dermatitis

Animals ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 2985
Author(s):  
Eleonora Elisa Alice Guidi ◽  
Alessandro Gramenzi ◽  
Paola Persico ◽  
Roberta Di Prinzio ◽  
Daniele Di Simone ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Gastrointestinal Tract ◽  
Multimodal Treatment ◽  
Clinical Manifestations ◽  
Skin Lesions ◽  
Cutaneous Lesions ◽  
Dietary Regimen ◽  
Canine Atopic Dermatitis ◽  
Intestinal Dysbiosis ◽  
Allergic Skin

Background: an imbalance of the intestinal microbiota can cause health problems in the gastrointestinal tract and in other organs. Canine Atopic Dermatitis (CAD) is a genetically predisposed, inflammatory and pruritic allergic skin disease with multifactorial etiology and multimodal treatment. The aim of this study was to assess the effect of a nutraceutical product on Dysbiotic Index (DI) and the skin lesions of atopic dogs. Methods: a nutraceutical product was administered to 32 dogs with CAD. The product was associated with a standardized hypoallergenic diet for 60 days; the dietary regimen continued for 120 days, while ongoing therapies remained unchanged. Values of Visual Analogic Scale (VAS), Canine Atopic Dermatitis Lesional Index (CADLI) and DI were evaluated on day 0, 60, 120. Results: all the 32 dogs showed a statistically significant decrease (p < 0.001) to V60 of VAS and CADLI, which persisted and increased to V120 when diet alone was continued. The decrease in the DI value was also statistically significant (p < 0.001). Conclusion: the intake of nutraceutical associated with diet resulted in a decrease in the index of intestinal dysbiosis, with an improvement in the subjective severity of cutaneous lesions.

Skin care in children with atopic dermatitis

2021 ◽  
Vol 16 (3) ◽  
pp. 134-140
Author(s):  
O.V. Goncharova ◽  
◽  
O.A. Komissarova ◽  
D.B. Kameldenova ◽  
L.A. Baryshnikova ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Normal Skin ◽  
Clinical Manifestations ◽  
Life Quality ◽  
Coconut Oil ◽  
Skin Lesions ◽  
Skin Care ◽  
Peloid Therapy ◽  
Unique Composition

In this article, we described specific features of baby skin and care for it (washing baby's face, bottom; cleaning ears, nose, and eyes; baby's bathing). We also assessed the efficacy of cream-balm ‘Tamba-PS’ (‘Adonis’ LLC, Russia) for the treatment of atopic dermatitis. ‘Tamba-PS’ contains only natural ingredients, including oil extract of native peloids from Lake Tambukan, plant extracts and essential oils, propolis, mumiyo, beeswax, honey, olive oil, sea buckthorn oil, coconut oil, and lecithin. We demonstrated its efficacy in infants and children aged 3 months to 3 years with both focal skin lesions and advanced lesions. Treatment improved children's quality of life, which was evaluated using the Dermatology Life Quality Index (DLQI) for children (from 22.8 ± 3.1 to 10.1 ± 2.0, р < 0.01); ‘socialization’ (according to the ‘KID-questionnaire,’ p ≤ 0.05) and the dynamics of clinical manifestations according to the SCORAD index (from 33.1 ± 2.9 to 14.2 ± 1.8 after therapy, p < 0.01). The unique composition of ‘Tamba-PS’ allows its daily use for problematic, irritated skin of children, as well as to protect normal skin from adverse effects, and as the external therapy for atopic dermatitis (the minimum course is 2 weeks (twice a day); the courses can be repeated if necessary). Key words: atopic dermatitis, Lake Tambukan mud, peloid therapy, skin care

scholarly journals Atopic Dermatitis-Like Skin Lesions Reduced by Topical Application and Intraperitoneal Injection of Hirsutenone in NC/Nga Mice

2010 ◽  
Vol 2010 ◽  
pp. 1-7 ◽  
Author(s):  
Mi Sook Jeong ◽  
Sun Eun Choi ◽  
Ji Young Kim ◽  
Jung Shim Kim ◽  
Eun Joo Kim ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Skin Lesions ◽  
Clinical Applications ◽  
Inflammatory Factors ◽  
Inflammatory Skin Disease ◽  
First Line ◽  
First Line Therapy ◽  
Line Therapy ◽  
Cox 2 ◽  
Effective Drugs

Atopic dermatitis (AD) is a common inflammatory skin disease. The increasing prevalence and severity of AD have prompted the developments of safer, more effective drugs. Although topical corticosteroids have been used as first line therapy for AD, their potential side effects limit their clinical applications. To investigate the effect of hirsutenone (HIR), a diarylheptanoid compound, on AD-like skin lesions and other factors related to immune response is the aim of this paper Th2-related cytokines (IL-4, IL-5, IL-13), eosinophil, IgE inflammatory factors (COX-2, iNOS) levels were reduced in blood, lymphocytes, and tissue after HIR treatment. These results suggest that HIR might be an effective treatment for AD.

Traditional Herbal Medicines, Newer Herbs and Other Novel Approaches Integrated in Herbal Medicine for Atopic Dermatitis-A Narrative Review

2020 ◽  
Vol 15 (3) ◽  
pp. 194-208
Author(s):  
Pravin Kumar ◽  
Dinesh Kumar Sharma ◽  
Mahendra Singh Ashawat
Keyword(s):  
Atopic Dermatitis ◽  
Herbal Medicines ◽  
Developed Countries ◽  
Skin Lesions ◽  
Scientific Data ◽  
Herbal Drugs ◽  
Biological Drugs ◽  
Alternative Treatment Option

Atopic Dermatitis (AD) is a prolonged reverting skin ailment with characteristically distributed skin lesions. In the previous decades, researchers had shown a marked interest in AD due to its increased prevalence in developed countries. Although different strategies including biological and immune modulators are available for the treatment of AD, each has certain limitations. The researchers had shown considerable interest in the management of AD with herbal medicines. The establishment of herbal drugs for AD might eliminate local as well as systemic adverse effects associated with long term use of corticosteroids and also higher cost of therapy with biological drugs. The present review discusses the traditional East Asian herbal medicines and scientific data related to newer herbal extracts or compositions for the treatment of AD. In vivo animal models and in vitro cell cultures, investigated with herbal medicines to establish a possible role in AD treatment, have also been discussed in the paper. The paper also highlights the role of certain new approaches, i.e. pharmacopuncture, a combination of allopathic and herbal medicines; and novel carriers (liposomes, cubosomes) for herbal drugs on atopic skin. In conclusion, herbal medicines can be a better and safe, complementary and alternative treatment option for AD.

Scabies: A Neglected Global Disease

2020 ◽  
Vol 16 (1) ◽  
pp. 33-42 ◽  
Author(s):  
Alexander K.C. Leung ◽  
Joseph M. Lam ◽  
Kin F. Leong
Keyword(s):  
English Language ◽  
Clinical Manifestations ◽  
Skin Lesions ◽  
Sarcoptes Scabiei ◽  
Clinical Findings ◽  
Effective Control ◽  
Search Term ◽  
Skin Contact ◽  
Intense Pruritus ◽  
Meta Analyses

Background: Scabies is a skin disease caused by an obligate human parasite mite Sarcoptes scabiei var. hominis. Children under the age of two and elderly individuals are at the greatest risk. Knowledge of this condition is important for an early diagnosis to be made and treatment to be initiated. Objective: The review aimed to familiarize physicians with the clinical manifestations, diagnosis, evaluation, and management of scabies. Methods: A search was conducted using Pubmed with the built-in "Clinical Queries" tool. The search term "Scabies" was used. The categories of "epidemiology", "diagnosis", "therapy", "prevention" and "prognosis" had a limited scope for primary clinical studies. Meta-analyses, randomized controlled trials, clinical trials, observational studies, and reviews were included. Only papers published in the English language were included. A descriptive, narrative synthesis was provided of the retrieved articles. Results: Worldwide, scabies affects 200 to 300 million individuals annually. The average prevalence is estimated to be 5 to 10% in children of developing countries. Transmission usually occurs after close prolonged skin-to-skin contact. Classic scabies is characterized by an erythematous papular eruption, serpiginous burrows, and intense pruritus. Sites of predilection include the webs of the fingers, volar wrists, lateral aspects of fingers, extensor surfaces of elbows and knees, waist, navel, abdomen, buttocks, groins, and, genitals. A clinical diagnosis of classic scabies can be made on the basis of the history and clinical findings. Other clinical variants include crusted scabies, nodular scabies, and bullous scabies. Finding the mite, ova, or fecal pellets on microscopic examination of scrapings taken from skin lesions confirms the diagnosis of scabies infestation. For eradication of scabies mites, the drugs of choice are topical permethrin and oral ivermectin. Conclusion: Scabies is a highly contagious parasitic cutaneous disease that is stigmatising and debilitating. Increased awareness, accurate diagnosis, and prompt treatment are essential for the effective control of scabies and for the prevention of the spread of the disease.

scholarly journals Impressic Acid Ameliorates Atopic Dermatitis-Like Skin Lesions by Inhibiting ERK1/2-Mediated Phosphorylation of NF-κB and STAT1

2021 ◽  
Vol 22 (5) ◽  
pp. 2334
Author(s):  
Jae Ho Choi ◽  
Gi Ho Lee ◽  
Sun Woo Jin ◽  
Ji Yeon Kim ◽  
Yong Pil Hwang ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Skin Lesions ◽  
Histopathological Analysis ◽  
Mrna Levels ◽  
Chemokine Production ◽  
Dermal Thickness ◽  
Skin Symptoms ◽  
Tnf Α ◽  
Ifn Γ ◽  
In Cells

Impressic acid (IPA), a lupane-type triterpenoid from Acanthopanax koreanum, has many pharmacological activities, including the attenuation of vascular endothelium dysfunction, cartilage destruction, and inflammatory diseases, but its influence on atopic dermatitis (AD)-like skin lesions is unknown. Therefore, we investigated the suppressive effect of IPA on 2,4-dinitrochlorobenzene (DNCB)-induced AD-like skin symptoms in mice and the underlying mechanisms in cells. IPA attenuated the DNCB-induced increase in the serum concentrations of IgE and thymic stromal lymphopoietin (TSLP), and in the mRNA levels of thymus and activation regulated chemokine(TARC), macrophage derived chemokine (MDC), interleukin-4 (IL-4), interleukin-5 (IL-5), interleukin-13 (IL-13), tumor necrosis factor-alpha (TNF-α) and interferon-gamma (IFN-γ) in mice. Histopathological analysis showed that IPA reduced the epidermal/dermal thickness and inflammatory and mast cell infiltration of ear tissue. In addition, IPA attenuated the phosphorylation of NF-κB and IκBα, and the degradation of IκBα in ear lesions. Furthermore, IPA treatment suppressed TNF-α/IFN-γ-induced TARC expression by inhibiting the NF-κB activation in cells. Phosphorylation of extracellular signalregulated protein kinase (ERK1/2) and the signal transducer and activator of transcription 1 (STAT1), the upstream signaling proteins, was reduced by IPA treatment in HaCaT cells. In conclusion, IPA ameliorated AD-like skin symptoms by regulating cytokine and chemokine production and so has therapeutic potential for AD-like skin lesions.

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