scholarly journals Identification and Antibacterial Resistance of Bacteria Isolated from Poultry

2012 ◽  
Vol 61 (4) ◽  
pp. 323-326 ◽  
Author(s):  
FADI. E. EL-RAMI ◽  
FAWWAK. T. SLEIMAN ◽  
LEXANDER. M. ABDELNOOR
Keyword(s):  
Antibacterial Agents ◽  
Antibacterial Resistance ◽  
Bacterial Strains ◽  
Growth Promoters ◽  
E Coli ◽  
Food Borne Pathogens ◽  
Food Borne ◽  
Antibiotic Growth Promoters ◽  
Food Borne Infections ◽  
Potential Food

Food-borne infections are among the prominent health hazards. Antibacterial agents (ABA) are usually administered to poultry in Lebanon as antibiotic growth promoters (AGP), which might lead to the dissemination of resistant bacterial strains. The aims of this study were to isolate potential food borne pathogens from poultry and investigate an association between AGP usage and antibacterial resistance (ABR). Isolates were obtained from the culture of cloacae swabs and identified. Escherichia coli was the predominant isolate. There was a significant association between the use of tetracycline and gentamicin as AGP and the number of E. coli isolates resistant to these ABA.

Ability of Bdellovibrio bacteriovorus 109J to Lyse Gram-Negative Food-Borne Pathogenic and Spoilage Bacteria

1995 ◽  
Vol 58 (2) ◽  
pp. 160-164 ◽  
Author(s):  
PINA M. FRATAMICO ◽  
RICHARD C. WHITING
Keyword(s):  
Coli Strain ◽  
Gram Negative Bacteria ◽  
Bacterial Strains ◽  
Bdellovibrio Bacteriovorus ◽  
Gram Negative ◽  
E Coli ◽  
Food Borne Pathogens ◽  
Food Borne ◽  
Spoilage Bacteria ◽  
Predatory Bacteria

Bdellovibrios are a group of aerobic, predatory bacteria which attack, penetrate and grow in many species of gram-negative bacteria, causing the lysis of the invaded prey organism. Bdellovibrio bacteriovorus strain 109J varied in its ability to lyse 32 bacterial strains comprising six genera of food-borne pathogens and spoilage organisms. The reduction in the levels of the prey bacteria ranged from 0.1 to 7.7 log-values after 7 h of incubation at 30°C. Escherichia coli strain 2239-69 (pathogenic serotype 026:H11) was lysed most effectively at temperatures between 30 and 37°C, however, lysis also occurred at 12 and 19°C when the incubation period was extended to 24 h. Bdellovibrio bacteriovorus was effective in reducing the level of E. coli 2239-69 at pH 5.6 to 8.6. Increasing the Bdellovibrio: E. coli ratio resulted in a more rapid E. coli reduction. This study demonstrated the potential usefulness of bdellovibrios for the biological control of pathogenic and spoilage organisms in foods.

scholarly journals A Rapid and Sensitive Europium Nanoparticle-Based Lateral Flow Immunoassay Combined with Recombinase Polymerase Amplification for Simultaneous Detection of Three Food-Borne Pathogens

2021 ◽  
Vol 18 (9) ◽  
pp. 4574
Author(s):  
Kai Chen ◽  
Biao Ma ◽  
Jiali Li ◽  
Erjing Chen ◽  
Ying Xu ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Vibrio Parahaemolyticus ◽  
Pathogenic Bacteria ◽  
Simultaneous Detection ◽  
Lateral Flow ◽  
Recombinase Polymerase Amplification ◽  
Quantitative Detection ◽  
Bacterial Strains ◽  
Food Borne Pathogens ◽  
Food Borne

Food-borne pathogens have become an important public threat to human health. There are many kinds of pathogenic bacteria in food consumed daily. A rapid and sensitive testing method for multiple food-borne pathogens is essential. Europium nanoparticles (EuNPs) are used as fluorescent probes in lateral flow immunoassays (LFIAs) to improve sensitivity. Here, recombinase polymerase amplification (RPA) combined with fluorescent LFIA was established for the simultaneous and quantitative detection of Listeria monocytogenes, Vibrio parahaemolyticus, and Escherichia coliO157:H7. In this work, the entire experimental process could be completed in 20 min at 37 °C. The limits of detection (LODs) of EuNP-based LFIA–RPA were 9.0 colony-forming units (CFU)/mL for Listeria monocytogenes, 7.0 CFU/mL for Vibrio parahaemolyticus, and 4.0 CFU/mL for Escherichia coliO157:H7. No cross-reaction could be observed in 22 bacterial strains. The fluorescent LFIA–RPA assay exhibits high sensitivity and good specificity. Moreover, the average recovery of the three food-borne pathogens spiked in food samples was 90.9–114.2%. The experiments indicate the accuracy and reliability of the multiple fluorescent test strips. Our developed EuNP-based LFIA–RPA assay is a promising analytical tool for the rapid and simultaneous detection of multiple low concentrations of food-borne pathogens.

Introduction to the Food Safety Concerns of Verotoxin-Producing Escherichia coli

2003 ◽  
Vol 228 (4) ◽  
pp. 331-332 ◽  
Author(s):  
Hussein S. Hussein ◽  
Stanley T. Omaye
Keyword(s):  
Escherichia Coli ◽  
Food Safety ◽  
Safety Concern ◽  
Critical Evaluation ◽  
The United States ◽  
Global Perspective ◽  
E Coli ◽  
The Past ◽  
Food Borne Pathogens ◽  
Food Borne

Verotoxin-producing Escherichia coli (VTEC) have emerged in the past two decades as food-borne pathogens that can cause major outbreaks of human illnesses worldwide. The number of outbreaks has increased in recent years due to changes in food production and processing systems, eating habits, microbial adaptation, and methods of VTEC transmission. The human illnesses range from mild diarrhea to hemolytic uremic syndrome (HUS) that can lead to death. The VTEC outbreaks have been attributed to O157:H7 and non-O157:H7 serotypes of E. coli. These E. coli serotypes include motile (e.g., O26:H11 and O104:H21) and nonmotile (e.g., O111:H–,0145:H–, and O157:H–) strains. In the United States, E. coli O157:H7 has been the major cause of VTEC outbreaks. Worldwide, however, non-O157:H7 VTEC (e.g., members of the 026, O103, O111, O118, O145, and O166 serogroups) have caused approximately 30% of the HUS cases in the past decade. Because large numbers of the VTEC outbreaks have been attributed to consumption of ruminant products (e.g., ground beef), cattle and sheep are considered reservoirs of these food-borne pathogens. Because of the food safety concern of VTEC, a global perspective on this problem is addressed (Exp Biol Med Vol. 228, No. 4). The first objective was to evaluate the known non-O157:H7 VTEC strains and the limitations associated with their detection and characterization. The second objective was to identify the VTEC serotypes associated with outbreaks of human illnesses and to provide critical evaluation of their virulence. The third objective was to determine the rumen effect on survival of E. coli O157:H7 as a VTEC model. The fourth objective was to explore the role of intimins in promoting attaching and effacing lesions in humans. Finally, the ability of VTEC to cause persistent infections in cattle was evaluated.

Acrobeloides buetschlii as a potential vector for enteric pathogens

Nematology ◽  
2015 ◽  
Vol 17 (4) ◽  
pp. 447-457 ◽  
Author(s):  
Yulia Kroupitski ◽  
Riky Pinto ◽  
Patricia Bucki ◽  
Edward Belausov ◽  
Liliane Ruess ◽  
...  
Keyword(s):  
Enteric Pathogens ◽  
Suitable Model ◽  
Human Pathogens ◽  
Relevant Model ◽  
Free Living ◽  
Potential Vector ◽  
E Coli ◽  
Food Borne Pathogens ◽  
Food Borne ◽  
Agricultural Setting

The objective of the present study was to assess ifAcrobeloides buetschlii, an opportunistic species common across many soils, can be employed as a suitable model for interactions between free-living soil nematodes and enteric human pathogens.Acrobeloides buetschliiwas exposed to mCherry-taggedSalmonella entericaandEscherichia coliO157:H7 and its vector potential was assessed.Salmonellacells were more readily ingested by the nematodes compared toE. coliO157:H7. Adult nematodes ingested more bacteria compared to juveniles.Salmonellasurvived internally for at least 7 days without affecting the viability of nematodes. Bacterial ingestion byA. buetschliidid not vary for three testedSalmonellaserovars but was significantly lower forE. coliO157:H7. Considering the ubiquitous nature of pathogen and vector, these findings suggest thatA. buetschliican serve as a relevant model for studying nematode-Salmonellainteractions in an agricultural setting and as potential transport for food-borne pathogens from soil to crops.

scholarly journals Sensitivity of Escherichia albertii, a Potential Food-Borne Pathogen, to Food Preservation Treatments

2007 ◽  
Vol 73 (13) ◽  
pp. 4351-4353 ◽  
Author(s):  
Manan Sharma ◽  
Kalmia E. Kniel ◽  
Alexandra Derevianko ◽  
Jason Ling ◽  
Arvind A. Bhagwat
Keyword(s):  
Diarrheal Disease ◽  
Food Preservation ◽  
Wild Type ◽  
E Coli ◽  
Acid Ph ◽  
Food Borne ◽  
Potential Food

ABSTRACT Escherichia albertii is a potential food-borne pathogen because of its documented ability to cause diarrheal disease by producing attachment and effacement lesions. Its tolerances to heat (56°C), acid (pH 3.0), and pressure (500 MPa [5 min]) were evaluated and found to be significantly less than those of wild-type E. coli O157:H7.

scholarly journals Escherichia coli and Salmonella spp. in meat in Jeddah, Saudi Arabia

2013 ◽  
Vol 7 (11) ◽  
pp. 812-818 ◽  
Author(s):  
Archana Iyer ◽  
Taha Kumosani ◽  
Soonham Yaghmoor ◽  
Elie Barbour ◽  
Esam Azhar ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gastrointestinal Diseases ◽  
Developed Countries ◽  
Salmonella Spp ◽  
E Coli ◽  
Food Borne Pathogens ◽  
Food Borne ◽  
Microbiological Methods ◽  
Meat Samples ◽  
Butcher Shops

Introduction: Food-borne pathogens are the leading cause of illness and death in developing countries, killing approximately 1.8 million people annually. In developed countries, food-borne pathogens are responsible for millions of cases of infectious gastrointestinal diseases each year, costing billions of dollars. The objective of this study was to screen for two major food-borne pathogens, Escherichia coli and Salmonella spp., from meat samples obtained from different strata of the consumer market in Jeddah. Methodology: A total of 60 meat samples, 20 each from large hypermarkets, groceries and small butcher shops were used in the study. Samples were transported to the laboratory in a cooler. They were macerated in peptone water and then seeded on selective media appropriate for each organism. Colonies were identified using conventional microbiological methods and suspected colonies were confirmed as E. coli and Salmonella spp. by polymerase chain reaction (PCR) using specific primers. Results: The results indicated a high degree of contamination in samples from butcher shops as compared to those from groceries or hypermarkets (high scale supermarkets). Both pathogens E. coli and Salmonella spp. were found in higher rates in the samples from butcher shops. In small butcher shops, E. coli was found at an incidence of 65%, and Salmonella at 45%. Conclusion: The results indicate an urgent need for applying proper food hygienic practices in food outlets, especially in small ones, to reduce the incidence of food-borne diseases. Vigilance by the right agencies must be implemented in order to prevent future food-borne outbreaks.

scholarly journals Essential oils as antibacterial agents against food-borne pathogens: Are they really as useful as they are claimed to be?

2017 ◽  
Vol 54 (13) ◽  
pp. 4344-4352 ◽  
Author(s):  
M. I. S. Santos ◽  
S. R. Martins ◽  
C. S. C. Veríssimo ◽  
M. J. C. Nunes ◽  
A. I. G. Lima ◽  
...  
Keyword(s):  
Essential Oils ◽  
Antibacterial Agents ◽  
Food Borne Pathogens ◽  
Food Borne

scholarly journals 1,4-Naphthoquinone Analogues: Potent Antibacterial Agents and Mode of Action Evaluation

Molecules ◽  
2019 ◽  
Vol 24 (7) ◽  
pp. 1437 ◽  
Author(s):  
Palanisamy Ravichandiran ◽  
Sunirmal Sheet ◽  
Dhanraj Premnath ◽  
Ae Rhan Kim ◽  
Dong Jin Yoo
Keyword(s):  
Antibacterial Activity ◽  
Mode Of Action ◽  
Bacterial Infections ◽  
Antibacterial Agents ◽  
Annexin V ◽  
Ros Generation ◽  
Redox Potentials ◽  
Bacterial Strains ◽  
E Coli ◽  
New Class

1,4-Naphthoquinones have antibacterial activity and are a promising new class of compound that can be used to treat bacterial infections. The goal was to improve effective antibacterial agents; therefore, we synthesized a new class of naphthoquinone hybrids, which contain phenylamino-phenylthio moieties as significant counterparts. Compound 4 was modified as a substituted aryl amide moiety, which enhanced the antibacterial activity of earlier compounds 3 and 4. In this study, five bacterial strains Staphylococcus aureus (S. aureus), Listeria monocytogenes (L. monocytogenes), Escherichia coli (E. coli), Pseudomonas aeruginosa (P. aeruginosa) and Klebsiella pneumoniae (K. pneumoniae) were used to evaluate the antibacterial potency of synthesized naphthoquinones using the minimal inhibitory concentration (MIC) method. Most of the studied naphthoquinones demonstrated major antibacterial activity with a MIC of 15.6 µg/mL–500 µg/mL. Selected compounds (5a, 5f and 5x) were studied for the mode of action, using intracellular ROS generation, determination of apoptosis by the Annexin V-FITC/PI assay, a bactericidal kinetic study and in silico molecular modelling. Additionally, the redox potentials of the specified compounds were confirmed by cyclic voltammetry (CV).

scholarly journals Effect of a bovine colostrum supplementation in piglet diet at weaning on growth performances, food ingestion and faecal E. coli concentrations

2005 ◽  
Vol 2005 ◽  
pp. 85-85
Author(s):  
C. Boudry ◽  
I. Didderen ◽  
J. Wavreille ◽  
D. Portetelle ◽  
J-P Dehoux ◽  
...  
Keyword(s):  
High Availability ◽  
Bovine Colostrum ◽  
Food Ingestion ◽  
Weaned Piglets ◽  
Growth Promoters ◽  
High Concentration ◽  
E Coli ◽  
Growth Performances ◽  
Antibiotic Growth Promoters ◽  
Growth Promoting

In the perspective of the complete ban of antibiotic growth promoters use in animal food by 2006, many alternatives have been studied. However, most of them are not yet technically and/or economically competitive with antibiotics. In this way, the incorporation of bovine colostrum in piglet diets has been studied. Bovine colostrum was chosen for its high concentration in growth promoting and anti-microbial peptides (Playford et al., 2000) and for its high availability. The aims of this study were to measure the efficiency of a bovine colostrum supplementation on growth performances of newly weaned piglets and to evaluate its effects on the digestive and immunological troubles involved by weaning (Pluske et al., 1997 and Vega-Lopez et al., 1995).

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