scholarly journals Biological Properties of Silver Nanoparticles from β (1-3) Glucan Isolated from the Edible Mushroom Pleurotus florida

2020 ◽  
Vol 10 (2) ◽  
pp. 2096-2106
Keyword(s):  
Silver Nanoparticles ◽  
Drug Development ◽  
Hydroxyl Radical ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Edible Mushroom ◽  
Hot Water ◽  
Dependent Manner ◽  
Pleurotus Florida ◽  
Radical Scavenging Assay

Pleurotus florida of the genus Pleurotus is a delicious edible mushroom with high therapeutic potential. Silver nanoparticles (AgNPs) are gaining a lot of importance nowadays because of their wide industrial and biomedical applications. Capping of AgNPs with polysaccharides, the most potent mushroom derived metabolites, is promising towards natural drug development for free radical-induced ailments. In the present investigation, silver nanoparticles were synthesized using β-glucan isolated from P. florida, and their antioxidant properties were studied. Polysaccharide (PS) isolation was carried out by hot water extraction, alcohol precipitation, deproteinization, and dialysis. Preliminary characterization of PS by Anthrone, Lowry’s method, Paper Chromatography, and FT-IR showed that PS is protein-bound β-(1-3) - Glucan. Synthesis of Silver nanoparticle from PS (PS-AgNPs) was confirmed by UV spectroscopy. Antioxidant properties of PS and PS-AgNPs were evaluated by Total antioxidant capacity assay, DPPH assay, Total reducing power, and Hydroxyl radical scavenging assay. Both PS and PS-AgNPs showed profound antioxidant activity in a dose-dependent manner, and PS-AgNPs was more active than PS. The highest activity was shown in Hydroxyl radical scavenging assay in which PS and PS-AgNPs showed 81.8% and 89.4% activity, respectively, at the highest tested dose. The present study revealed the possible use of P. florida in antioxidant drug development.

scholarly journals Antioxidant Potential of Silver Nanoparticles Synthesized from Βeta (1-3) Glucan Isolated from the Edible Mushroom Pleurotus florida

2020 ◽  
Vol 2 (1) ◽  
pp. 79
Keyword(s):  
Silver Nanoparticles ◽  
Hydroxyl Radical ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Edible Mushroom ◽  
Hot Water ◽  
Dependent Manner ◽  
Preliminary Characterization ◽  
Pleurotus Florida ◽  
Radical Scavenging Assay

Pleurotus florida of the genus Pleurotus, is a delicious edible mushroom with high therapeutic potential and is being cultivated extensively in many parts of the world, including India. Polysaccharides, especially β-glucans, are the most potent mushroom derived metabolites. Silver nanoparticles (AgNPs) are gaining a lot of importance nowadays because of their wide industrial and biomedical applications. In the present investigation, silver nanoparticles were synthesized using β-glucan isolated from the fruiting bodies of P. florida and their antioxidant properties were studied. Polysaccharide (PS) isolation was carried out by hot water extraction, deproteinization, alcohol precipitation, centrifugation, and dialysis. Preliminary characterization of isolated polysaccharide was done by the Anthrone method, Lowry’s method, Thin Layer Chromatography (TLC), and FT-IR. The PS was treated with AgNO3 solution (0.001M) for the biosynthesis of silver nanoparticles, and nanoparticle formation was confirmed by UV spectroscopy. Antioxidant properties of the PS, as well as synthesized nanoparticles, were evaluated using Total antioxidant capacity assay, DPPH-Free radical scavenging assay, Total reducing power assay, and Hydroxyl radical scavenging assay. Preliminary characterization showed that the isolated PS is protein-bound β (1-3) Glucan. Both the PS and silver nanoparticles showed profound antioxidant activity in a dose-dependent manner. In all assays, silver nanoparticles showed more activity than the PS. The highest activity was shown in Hydroxyl radical scavenging assay in which PS and nanoparticles showed 81.8% and 89.4% activity, respectively, at the highest tested dose of 2000µg. The present study reveals the possibility of β-glucan -silver nanoparticles from P. florida as a suitable candidate for antioxidant drug development.

Strong antihemolytic and antioxidant properties of aqueous extract from Algerian Hammada elegans (Bge.) Botsch (Chenopodiaceae)

2021 ◽  
Vol 17 ◽  
Author(s):  
Brahim Asseli ◽  
Reguia Mahfoudi ◽  
Amar Djeridane ◽  
Mohamed Yousfi
Keyword(s):  
Free Radical ◽  
Aqueous Extract ◽  
Radical Scavenging Activity ◽  
Condensed Tannin ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Dependent Manner ◽  
Vitro Assay

Background: Research on medicinal plant antioxidants has emerged as a potential therapeutic to prevent free radical generated damage in the human body. Hammada elegans Botsch (popularly known as “Ajram”) is a xerophytic plant widely found in Laghouat region, but there are only a few reports about the biological or chemical properties of these species. Hence, the aim of this study is to investigate the antioxidant and the antihemolytic activities of hexanic, acetonic, methanolic and aqueous extracts of aerial parts of Algerian Hammada elegans Botsch by employing different in vitro assay systems. Methods: The total phenolic content, the flavonoid content and the condensed tannin amount were analyzed using Folin-Ciocalteu, aluminum chloride and vanillin assays, respectively. The in vitro antioxidant capacity of extracts was assessed by CUPRAC, iron chelating, ABTS•+and antihemolytic assays, and was expressed as EC50 values. Results: Among the analyzed extracts, the aqueous extract had the highest phenolic, flavonoid and tannin contents. Also, this extract displayed the highest antioxidant capacities compared to the other extracts and standards. Its EC50 value for ABTS radical-scavenging activity was 0.265 ± 0.003 mg/L. Moreover, this extract showed high iron (II) chelating ability (EC50 = 0.958 ± 0.001 mg/L), and good antioxidant activity in the cupric ion reducing activity (CUPRAC) in a concentration dependent manner (EC50 were 0.709 ± 0.002 mg/L). Additionally, this extract had the best antihemolytic activity against AAPH-induced hemolysis (EC50=0.090 ± 0.004 mg/L). Conclusion: Our study revealed that the aqueous extract of Hammada elegans Botsch, is a potential source of antioxidants which possess a high protective effect of membrane against free radical.

scholarly journals In vitro inhibitory potentials of ethanolic extract of Moringa oleifera flower against enzymes activities linked to diabetes

2021 ◽  
Vol 10 (4) ◽  
pp. 408-414
Author(s):  
Oluwaseun Ruth Olasehinde ◽  
Olakunle Bamikole Afolabi ◽  
Benjamin Olusola Omiyale ◽  
Oyindamola Adeniyi Olaoye
Keyword(s):  
Free Radical ◽  
Moringa Oleifera ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Antidiabetic Activity ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Antioxidant Power

Introduction: Diabetes mellitus (DM) has been recognized as the seventh leading cause of global mortality; however, researchers seek alternative means to manage the menace. The current study sought to investigate antioxidant potentials, α-amylase, and α-glucosidase inhibitory activities of ethanolic extract of Moringa oleifera flower in vitro. Methods: Antioxidant properties of the extract were appraised by assessing its inhibition against 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl (OH•), and hydrogen peroxide (H2O2) free radicals, as well as ferric reducing antioxidant power (FRAP), the antidiabetic activity was evaluated by α-amylase and α-glucosidase inhibition.Results: In this study, ethanolic extract of M. oleifera flower demonstrated a significant (P < 0.05) inhibition against DPPH free radical (43.57–83.56%) in a concentration-dependent manner, while FRAP (101.76 ± 1.63 mg/100 g), OH• scavenging ability (71.62 ± 0.95 mg/100 g), and H2O2 free radical scavenging capacity (15.33 ± 1.20 mg/100 g) were also observed. In the same manner, ethanolic extract of M. oleifera flower revealed a significant (P < 0.05) inhibition against α-amylase (IC50= 37.63 mg/mL) and α-glucosidase activities (IC50= 38.30 mg/mL) in the presence of their respective substrates in a concentration-dependent manner in comparison with acarbose. Conclusion: Ethanoic extract of M. oleifera flower could be useful as an alternative phytotherapy in the management of DM, having shown a strong antioxidative capacity and substantial inhibition against the activities of key enzymes involved in carbohydrate hydrolysis in vitro.

Mycochemical composition and antioxidant potential of Macrolepiota africana (Heim) Heinem. (Agaricaceae), an edible mushroom from Gabon

2021 ◽  
Vol 2 (1) ◽  
pp. 028-033
Author(s):  
Hugues Calixte Eyi Ndong ◽  
André Ledoux Njouonkou
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Activity Index ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Edible Mushroom ◽  
Mushroom Species ◽  
Chemical Screening ◽  
Ethanolic Extracts ◽  
Pharmacological Potential

Macrolepiota africana is a fungus used as food in Gabon for its culinary properties. However, its nutritional value, mycochemical contents, antioxidant properties and health potential still unknown. The present study investigated the phytochemicals and antioxidant properties of this mushroom species. This chemical screening was followed by a study of the antioxidant activity and a prediction of additional pharmacological activities of M. africana. Using standard methodology, the mycochemical analyses were carried out on aqueous, hydro-ethanolic and ethanolic fungi extracts. The antioxidant activity of the mushroom extracts was determined using DPPH radical scavenging assay. Apart from Digitoxigenine, flavonol and gitoxigenine that were not found in any of the extracts, all other tested mycochemical were found in atleast one of the extracts. Alkaloids, flavonoids, polyphenols, oses and holosides, proanthocyanidins and coumarins were found in all extracts at different intensity. Saponosids, sterols and triterpenes, tannins gallics, reducing sugar, anthracenosides and digitoxine were found in two extracts while tannins catechics, flavone and gitoxine were detected only in one extract. The dosage of phenolic compounds confirmed the richness of this fungus in total polyphenols, its moderate richness in proanthocyanidins, the lack of flavonoids in the aqueous extract and the moderate richness of the hydro-ethanolic and ethanolic extracts in flavonoids. Regarding the antioxidant activities, the results obtained for the DPPH trapping test showed that the different extracts had low to moderate antioxidant activity with antioxidant activity index (IAA) ranged 0.29 to 0.97 respectively in aqueous and ethanolic extracts. The presence of these mycochemical compounds along with the identified antioxidant activities shows that this M. africana have some pharmacological potential.

scholarly journals IN VITRO ANTICOAGULANT AND ANTIOXIDANT ACTIVITIES OF PRASAPLAI RECIPE AND ZINGIBER CASSUMUNAR ROXB. EXTRACTS

2019 ◽  
pp. 26-30
Author(s):  
SURIYAN SUKATI ◽  
KHEMJIRA JARMKOM ◽  
SURACHAI TECHAOEI ◽  
NAKUNTWALAI WISIDSRI ◽  
WARACHATE KHOBJAI
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Anticoagulant Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Coagulation Factor ◽  
Hot Water ◽  
Aqueous Extracts ◽  
Antioxidant Power ◽  
Zingiber Cassumunar

Objective: This present study aimed to evaluate the anticoagulant activity and antioxidant properties of Prasaplai recipe (PPR), a Thai traditionalmedicine, and its major ingredient, Zingiber cassumunar (ZC) Roxb. extracts, seeking new therapeutic purposes for the recipe.Methods: Aqueous extracts of PPR and ZC Roxb. were prepared by hot water decoction technique. The anticoagulant activity of the extracts wasevaluated by prothrombin time (PT) and activated partial thromboplastin time (APTT) tests. In addition to anticoagulant activity, total phenolcontent and antioxidant activity were investigated. Total phenol content was determined using the Folin–Ciocalteu assay. The antioxidant activity wasestimated by DPPH radical scavenging activity and ferric reducing antioxidant power assay.Results: The APTT of plasma samples mixed with the PPR and ZC Roxb. extracts was significantly prolonged (p<0.05) at the concentration of1.0 mg/ml and above comparing to the control (normal saline solution) but was no significantly different for the PT. These results suggested thatPPR and ZC Roxb. extracts showed anticoagulant activity affecting the function of coagulation factor in the intrinsic pathway. All aqueous extractspossessed considerable antioxidant activity and were rich in total polyphenol.Conclusion: This finding indicates that the aqueous extracts possess significant anticoagulant and antioxidant activities, thus showing the potentialPPR and ZC Roxb. as a new source of bioactive compounds for therapeutic purposes, with particular emphasis on the prevention and treatment ofthrombosis.

scholarly journals Antioxidant Capacity and the Correlation with Major Phenolic Compounds, Anthocyanin, and Tocopherol Content in Various Extracts from the Wild EdibleBoletus edulisMushroom

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Emanuel Vamanu ◽  
Sultana Nita
Keyword(s):  
Antioxidant Capacity ◽  
Antioxidant Properties ◽  
Reducing Power ◽  
Ethanolic Extract ◽  
Edible Mushroom ◽  
Tocopherol Content ◽  
Hot Water ◽  
Antioxidant Compounds ◽  
Methanolic Extracts ◽  
Freeze Dried

Boletus edulisis a wild edible mushroom habitually consumed by rural populations. Ethanolic and methanolic extracts was obtained in cold and hot water from dried fruit bodies. The antioxidant activity of freeze-dried extracts fromB. eduliswere investigated using free radicals scavenging activity, reducing power, metal chelating effect, inhibition of lipid peroxidation, and the identification of antioxidant compounds. The levels of different compounds with antioxidant properties were higher in alcoholic extracts compared with aqueous extracts. Rosmarinic acid was the major phenolic compound, it being identified in a concentration between7±0.23and56±0.15 mg/100 g extract. A positive correlation between the content of total phenols, flavonoids, anthocyanins, and tocopherols, and the antioxidant capacity of the extracts was determined. The results showed that the ethanolic extract of Romanian wild mushroomB. edulisrepresents a natural source of functional compounds.

scholarly journals Evaluation of antioxidant capacity of polysaccharide in Jerusalem artichoke (Helianthus tuberosus L.) during overwintering

2019 ◽  
Vol 78 ◽  
pp. 02008
Author(s):  
Hong-li Zhou ◽  
Bing Li ◽  
Mei-fu Wu ◽  
Ye Liu
Keyword(s):  
Hydroxyl Radical ◽  
Antioxidant Capacity ◽  
Mass Concentration ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Jerusalem Artichoke ◽  
Dpph Radical ◽  
Before And After ◽  
Dpph Radical Scavenging ◽  
Radical Scavenging Assay

To determine the effect of different harvesting time on antioxidant capacity in Jerusalem artichoke polysaccharides (inulin), the Jerusalem artichoke before and after overwintering were collected from the same region, and then evaluate their antioxidant capacity in vitro by reducing power assay, DPPH radical-scavenging assay and hydroxyl radical-scavenging assay. Ascorbic acid at similar mass concentration was served as positive control. The results showed no significant difference were observed in reducing power when the absorbance values were about 0.3. When the mass concentrations before and after overwintering were 0.9 mg/mL and 1.7 mg/mL, the DPPH radical-scavenging rate both reached at 50%. And when the mass concentrations were both 10 mg/mL, the hydroxyl radical-scavengings were 35.8% and 27.9%, respectively. Thus, it could be concluded that the antioxidant capacity in Jerusalem artichoke polysaccharides before overwintering was higher than that after overwintering, and showed a good dose-dependent manner with its mass concentration.

scholarly journals In vitro Cancer Cell Growth Inhibition and Antioxidant Activity of Bombax ceiba (Bombacaceae) Flower Extracts

2014 ◽  
Vol 9 (5) ◽  
pp. 1934578X1400900 ◽  
Author(s):  
Rosa Tundis ◽  
Khaled Rashed ◽  
Ataa Said ◽  
Francesco Menichini ◽  
Monica R. Loizzo
Keyword(s):  
Cancer Cell ◽  
Antiproliferative Activity ◽  
Human Cancer ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Dependent Manner ◽  
Bleaching Test ◽  
Β Carotene

The flowers of Bombax ceiba were investigated for their chemical composition, antioxidant effects and antiproliferative activity against seven human cancer cell lines. The antiproliferative responses of diethyl ether (DE) and light petroleum (PE) extracts were evaluated by sulforhodamine B (SRB) assay against MCF-7, HeLa, COR-L23, C32, A375, ACHN, and LNCaP cells in comparison with a human normal cell line, 142BR. Moreover, extracts were characterized by GC-MS analysis and tested for their antioxidant properties by different in vitro systems, namely DPPH, Fe-chelating activity and β-carotene bleaching test. Both PE and DE extracts showed the highest antiproliferative activity against human renal adenocarcinoma (ACHN) in a concentration-dependent manner. PE extract showed the highest radical scavenging activity against the DPPH radical, while DE extract was more active in the β-carotene bleaching test. The presence of β-sitosterol and some fatty acids may contribute to the bioactivity of B. ceiba flower extracts.

scholarly journals Phytochemical evaluation and in vitro antioxidant studies of selected species of Gomphostemma Wall. ex Benth. from Western Ghats

2018 ◽  
Vol 8 (6-s) ◽  
pp. 32-37
Author(s):  
M. K Sajitha ◽  
J. E. Thoppil
Keyword(s):  
Hydroxyl Radical ◽  
Western Ghats ◽  
Quantitative Estimation ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antioxidant Potential ◽  
Aluminium Chloride ◽  
Dpph Assay ◽  
Radical Scavenging Assay

Plants are a rich source of bioactive compounds and have played a vital role in traditional medicine. Plant derived compounds are proved to have less side effects as compared to synthetic compounds. However, a truncated history on the contribution of plants to medicine together with lack of validation of plant derived components have led to massive research in phytochemistry and allied fields. Present study was intended to identify as well as to assess and compare different phytochemicals and antioxidant potential of selected taxa of Gomphostemma endemic to Western Ghats. Methanolic extracts of aerial parts of Gomphostemma heyneanum var. heyneanum, Gomphostemma heyneanum var. rottleri and Gomphostemma eriocarpum were subjected to preliminary phytochemical screening. Quantitative estimation of phenols and flavonoids was done with Folin-Ciocalteu method and modified Aluminium chloride method respectively. Antioxidant potential was examined by DPPH assay, Hydroxyl radical scavenging assay and Reducing power activity. Preliminary screening indicated the presence of many secondary metabolites like phenols, flavonoids, alkaloids, terpenoids and steroids. Results of quantitative estimation revealed that G. heyneanum var. heyneanum has the highest concentration of phenols and flavonoids. Antioxidant potential determined in terms of inhibition percentage also displayed that Gomphostemma species have significant radical scavenging activity. The antioxidant potential exhibited by the plant species may be attributed to their phenol and flavonoid content. Keywords: Phytochemical constituents, Gomphostemma, Quantitative estimation, Total phenolics, Flavonoids, Antioxidant activity, DPPH assay, Hydroxyl radical scavenging assay, Reducing power activity.

scholarly journals Free Radical Scavenging and Anti-Inflammatory Activity of Chlorogenic Acid Mediated Silver Nanoparticle

2020 ◽  
pp. 106-112
Author(s):  
Anu Iswarya Jaisankar ◽  
Lakshminarayanan Arivarasu
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Chlorogenic Acid ◽  
Silver Nanoparticle ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Hazardous Substances ◽  
Oxidation Activity ◽  
Antioxidant Agents ◽  
Anti Inflammatory

Introduction: Nanotechnology is a field of research and innovation concerned with building 'things' - generally, materials and devices on the scale of atoms and molecules. It is a booming field of this 21st century. The role of nano technology is becoming very crucial in nearly every aspect of life ranging from cosmetics to advanced bio technological approaches. In recent years, silver nanoparticles have gained considerable attention in the field of medicine. The green synthesis of nanoparticles eliminates the generation and use of hazardous substances and thus sustains non toxicity. Chlorogenic acids are phenolic compounds formed by the esterification of cinnamic acids. They exhibit various pharmacological Properties. Our study deals with the green synthesis of Chlorogenic acid mediated silver nanoparticles and assessment of their anti inflammatory and antioxidant properties.   Aim: The present study aims at assessing the antioxidant and anti inflammatory property of chlorogenic acid mediated silver nanoparticle and investigating the efficacy of Chlorogenic acid mediated Silver nanoparticle. Materials and Methods: The methodology includes Green synthesis of Chlorogenic acid mediated Silver nano particle synthesis followed by tests for Anti inflammation and Anti oxidation. Results: Both the Anti inflammatory and Anti oxidation activity of the chlorogenic acid mediated silver nanoparticle had shown a proportionate increase in activity with increasing concentration of the compound. Conclusion: Chlorogenic acid mediated silver nanoparticles have shown significant anti inflammatory and anti oxidation activity and they are considered as potent anti inflammatory and antioxidant agents.

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