Implication of some biochemical parameters on normal cyclic and repeat breeding process in Bangladeshi dairy cows

Repeat breeding is one the most common reproductive disorders that hinders favorable productivity in domestic animals. Repeat breeding in dairy cows not only affects the fertility of the cattle to a considerable extent but also incurs great economic loss to the farmer. Biochemical profile can indicate the nutritional status and help in diagnosis and management of infertility in animals. Present study explores the implication of some biochemical parameters (total protein, bilirubin and some macro minerals as calcium, inorganic phosphorus and magnesium) on normal cyclic breeding process of Bangladeshi dairy cows. Current observation indicates that the lower calcium, inorganic phosphorus and magnesium concentrations might be associated with the ovulatory disturbances in repeat breeder cows. On the other hand, total protein and bilirubin do not play a significant role in causing repeat breeding problem in those cows. Thus, this study provides supportive evidence about the importance of calcium, inorganic phosphorus and magnesium in the resumption of repeat breeding condition. Asian J. Med. Biol. Res. June 2020, 6(2): 305-310

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Hormonal Profile Study of Bangladeshi Crossbred Dairy Cows in Relation to Repeat Breeding

Science Letters ◽

10.47262/sl/8.3.132020012 ◽

2020 ◽

Vol 8 (3) ◽

pp. 100-103

Keyword(s):

Luteinizing Hormone ◽

Dairy Cows ◽

Follicle Stimulating Hormone ◽

Economic Loss ◽

Normal Functioning ◽

Normal Cycle ◽

Hormonal Profile ◽

Breeding Condition ◽

Profile Study ◽

Repeat Breeder

The repeat breeding condition affects both the fertility of cows and the economy of farmers and the nation. Normal levels of various biochemical constituents are indispensable for the normal functioning of various body systems of animals, including the reproductive system. The hormonal profile is an indicator of the reproductive status and aids in the prognosis and restoration of animal fertility. The present study explores the implication of four hormones (luteinizing hormone, follicle-stimulating hormone, human chorionic gonadotropin and prolactin) on the normal cycle breeding process of Bangladeshi normal cyclic cows and repeat breeder dairy cows. Among four hormones, the levels of luteinizing hormone and prolactin were significantly different between the normal cyclic cows and repeat breeder cows. The assessment of hormonal profile aids in ameliorating the repeat breeding conditions of the dairy cows. The findings of the present study could be applied in overcoming repeat breeding problems in dairy cows and thus, economic loss could be lessened.

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Hormonal Profile Study of Bangladeshi Crossbred Dairy Cows in Relation to Repeat Breeding

Science Letters ◽

10.47262//sl/8.3.132020012 ◽

2020 ◽

pp. 100-103

Keyword(s):

Luteinizing Hormone ◽

Dairy Cows ◽

Follicle Stimulating Hormone ◽

Economic Loss ◽

Normal Functioning ◽

Normal Cycle ◽

Hormonal Profile ◽

Breeding Condition ◽

Profile Study ◽

Repeat Breeder

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The effects of different anticoagulants on biochemical parameters in blood of dairy cows

Veterinarski glasnik ◽

10.2298/vetgl1502013b ◽

2015 ◽

Vol 69 (1-2) ◽

pp. 13-20

Author(s):

Branislava Belic ◽

Marko Cincovic ◽

Maja Dosenovic ◽

Dragica Stojanovic ◽

Zorana Kovacevic

Keyword(s):

Alkaline Phosphatase ◽

Dairy Cows ◽

Alkaline Phosphatase Activity ◽

Total Protein ◽

Biochemical Parameters ◽

Biochemical Analysis ◽

Blood Parameters ◽

Albumin Level ◽

Blood Samples ◽

Holstein Friesian

Routine analysis of blood parameters requires high precision. Therefore a significant number of methods and recommendations has been developed to ensure the obtaining of precise results. The aim of this study was to investigate whether the type of anticoagulant affects the values of biochemical parameters in the blood of dairy cows in relation to the values obtained from the serum. The study was carried out on 20 healthy cows of Holstein- Friesian breed in the second month of lactation. The blood was taken by venipuncture from v.coccigea. There were used five types of vacutainers as follows: for the serum separation, with heparin, EDTA, citrate, and fluoride. Samples from each cow were taken in all five types of vacutainers respectively. There was determined the concentration of: albumin, total protein, glucose, Ca, P, BHB, NEFA, urea, cholesterol, triglycerides, ALT, AST, AP, GGT and bilirubin. The results of the analysis of samples that contained anticoagulants were compared statistically with analysis in serum that was considered to be the control one. It was also calculated the percentage of deviation in average values of concentration of metabolites obtained from samples with different anticoagulants in regard to the serum value. The results of the investigation show that anticoagulants influence the values of biochemical parameters in the blood of cows. In the samples where heparin was used as anticoagulant there was found higher albumin level (deviation 4.1%) as well as total protein (1.4%), but lower value of alkaline phosphatase activity relative to the serum (-33%). In the samples in which EDTA was used there were significantly lower levels of total protein (-5.8%), Ca (-49.6%), P (-17.7%), AP (-32%) and higher value AST (10.6%) compared to serum. In the samples where citrate or fluoride were used as anticoagulants there were found lower values of total protein, albumin, glucose (only citrate), Ca, P, BHB, NEFA, urea (only citrate), cholesterol, AP and GGT (only fluoride) and lower bilirubin when using citrate or higher when using fluoride, in comparison to the serum. Deviations of biochemical parameters measured from blood samples that were in citrate or fluoride were as follows: total protein (-5.3%, -5.2%), albumin (-7%, -5.7%), glucose (only citrate -6.2%), Ca (-55%, -82%), P (-29%, -24%), BHB (-53%, -80%), NEFA (-62.1%, -79 , 4%), urea (only citrate, -25.5%), cholesterol (-28.6%, -28.4%), AP (-38%, -32%), and GGT (only -17 fluoride, 8%) and higher bilirubin (-22.8%, 64.4%). For routine clinical biochemical analysis of blood of cows it is the most reliable to use the samples with heparin as an anticoagulant, because the use of other anticoagulants gives large variations in the values of biochemical parameters in relation to serum.

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Serum proteinogram and biochemistry of Holstein cows in the peripartum period

Pesquisa Veterinária Brasileira ◽

10.1590/1678-5150-pvb-5819 ◽

2019 ◽

Vol 39 (5) ◽

pp. 342-347

Author(s):

Thaís G. Rocha ◽

Camila Bortoletto ◽

Daniela G. Silva ◽

Kalina M.M.G. Simplício ◽

Luiz F. Zafalon ◽

...

Keyword(s):

Alkaline Phosphatase ◽

Dairy Cows ◽

Total Protein ◽

Repeated Measures ◽

Transition Period ◽

Serum Levels ◽

Inorganic Phosphorus ◽

Holstein Cows ◽

Total Calcium ◽

Peripartum Period

ABSTRACT: In face of the few reports found in national literature analyzing the potential influence of parturition number in serum proteinogram and biochemical profile in the peripartum period of high yielding dairy cows, the aim of the present study was to comparatively evaluate the dynamics of these serum constituents’ concentrations in blood samples obtained from primiparous and multiparous Holstein cows, 60 and 30 days prepartum and in the day of parturition. Data were analyzed by repeated measures variance analysis (ANOVA) and differences between groups and moments were analyzed by Tukey’s test. Results were considered significant when P<0.05. Parity influenced levels of total protein, albumin, globulins, magnesium, cholesterol, which were higher in multiparous cows, as well as concentrations of ceruloplasmin, total calcium, chloride and alkaline phosphatase activity, which were higher in primiparous cows. Parturition influenced serum concentrations of ceruloplasmin (+58%), transferrin (-25%), haptoglobin (+33%), total protein (-17%), globulins (-25%), immunoglobulin A (-43%), immunoglobulin G (-24%), total calcium (-12%), inorganic phosphorus (-10%), chloride (+5%), sodium (+4%), cholesterol (-23%), triglycerides (-38.6%), as well as activities of aspartate aminotransferase (+14%) and alkaline phosphatase (+28%). A decrease in serum levels of total calcium, inorganic phosphorus, cholesterol and triglycerides was more pronounced in multiparous than in primiparous cows. These results demonstrate that the interpretation of proteinogram and serum constituents should take into consideration lactation number and the moment of parturition as relevant factors in high yielding dairy cows in the transition period.

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Prediction of birth and postpartum pathology in deep-calving heifers by biochemical parameters of blood

Scientific Messenger of LNU of Veterinary Medicine and Biotechnology ◽

10.32718/nvlvet10104 ◽

2021 ◽

Vol 23 (101) ◽

pp. 21-25

Author(s):

L. V. Koreyba

Keyword(s):

Blood Serum ◽

Total Protein ◽

Biochemical Parameters ◽

Mineral Metabolism ◽

Obstetric Care ◽

Calcium Content ◽

Inorganic Phosphorus ◽

The State ◽

Total Protein Content ◽

Carotene Content

The success of the control of obstetric diseases in cattle depends on the timely implementation of preventive measures, and, in cases of pathology, on diagnosis, effective obstetric care and treatment. A prominent place is given to blood tests among the methods that allow to objectively assess the state of health and the course of the pathological process in animals' bodies. The study of biochemical parameters of blood allows for the assessment of the state of metabolic processes in animals, with high accuracy in establishing the body's general condition and predicting complications and adjusting their prevention and therapy. The goal of the work: our work aimed to study the biochemical parameters of the blood of deep-calving heifers for the prediction, correction, and prevention of obstetric pathology during childbirth and puerperium. The research was carried out on deep-calving heifers of Holstein breed selected on the principle of analogues. Biochemical examination of blood serum was performed in the district laboratory of veterinary medicine. Indicators of protein and mineral metabolism were determined in the blood serum of deep- calving heifers: total protein, carotene, reserve alkalinity, inorganic phosphorus, albumin content, class M immunoglobulins. The obtained research results indicate that the total protein content corresponds to the norm in deep-calving heifers of the Holstein black-spotted breed. The protein coefficient exceeds the standard. There are pronounced deviations in the blood's biochemical parameters: low levels of carotene almost two-fold, reserve alkalinity – 1.91-fold, the total calcium content – by 16.08 % and the ratio of calcium to phosphorus by 17.78 %. Since the decrease in carotene content correlates with the predisposition to endometritis, the Holstein black-spotted breed's deep-calving heifers are prone to developing birth and postpartum pathology. For pregnant cows which are predisposed to postpartum obstetric pathology, that occur in weak parturition activity, failure to release the placenta, uterus subinvolution, endometritis and postpartum hypocalcaemia, unfavorable prognostication is the low concentration in blood plasma of general calcium, inorganic phosphorus and carotene.

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Застосування неорганічних та органічних сполук Со, Cu та Zn за їх недостатності у дійних корів

Scientific Messenger of LNU of Veterinary Medicine and Biotechnology ◽

10.15421/nvlvet7836 ◽

2017 ◽

Vol 19 (78) ◽

pp. 177-181

Author(s):

L.G. Slivinska ◽

V.I. Rusyn ◽

I.A. Maksymovych ◽

M.I. Leno ◽

B.O. Chernushkin ◽

...

Keyword(s):

Trace Elements ◽

Body Weight ◽

Dairy Cows ◽

Organic Compounds ◽

Total Protein ◽

Biochemical Parameters ◽

Total Calcium ◽

Ratio Of Components ◽

Experimental Group ◽

Cu And Zn

The results of research of certain biochemical parameters of blood in dairy cows for the prevention of insufficiency of Co, Cu and Zn by inorganic and organic compounds of trace elements are presented. The purpose of the work – to investigate the state of individual biochemical parameters of blood in dairy cows when using inorganic and organic compounds Co, Cu and Zn to prevent their insufficiency. The research material was the blood serum of experimental animals, which determined the content of total protein and the percentage of albumin, total calcium content, inorganic phosphorus, aspartateaminotransferase (AST) activity, alanineaminotransferase (ALT), alkaline phosphatase (AP).For research on dairy cows of the first experimental group, together with the feeds of the main diet, a mixture of trace elements sulphates was fed in the following ratio of components (mg/100 kg body weight): CuSO4 – 50, CoSO4 – 5, ZnSO4 – 120. Animals of the second experimental group fed a mixture of methionates and lysinates of trace elements in the following ratio of components (mg/ 100 kg of body weight): CuMet – 15, CuLis – 15, CoMet – 1, CoLis – 1, ZnMet – 35, ZnLis – 35. Feeding the mixture of compounds of fragile microelements was carried out by mixing them with mixed fodder, once a day, for 60 days. The evaluation of the research results was carried out at the beginning and at the end of the experiment.On the background of the deficit of Сobalt, Сuprum and Zinc, clinically diseased animals have hypoproteinemia, hypoalbuminemia, hypocalcemia, increased activity of AST, ALT and AP, indicating a metabolic abnormality in cows and development of microelementosis. It was established that the use of chelated cheeses in the form of methionates and lysinates of scarce microelements had a better therapeutic effect compared to inorganic compounds. The result of the application of chelate compounds of trace elements was a possible increase in the content of total protein in the blood serum, fractions of albumins, total calcium, decrease in the activity of AST, ALT and AP.

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Relationship between certain serum biochemical values and serostatus against Anaplasma marginale in dairy cows

Veterinary World ◽

10.14202/vetworld.2019.1858-1861 ◽

2019 ◽

Vol 12 (11) ◽

pp. 1858-1861

Author(s):

Myassar O. Alekish ◽

Zuhair Bani Ismail

Keyword(s):

Dairy Cows ◽

Total Protein ◽

Biochemical Parameters ◽

Anaplasma Marginale ◽

Fatty Degeneration ◽

Enzyme Linked Immunosorbent Assay ◽

Gamma Glutamyl Transferase ◽

Serum Samples ◽

Serum Biochemical ◽

Glutamyl Transferase

Aim: This study was conducted to evaluate the possible association between values of certain serum biochemical parameters and seropositivity against Anaplasma marginale in dairy cows. Materials and Methods: Serum samples from 60 seropositive and 40 seronegative cows were used to determine the values of beta-hydroxybutyrate (BHB), glucose, creatinine, blood urea nitrogen, total protein, albumin, alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine transaminase (ALT), lactate dehydrogenase (LDH), and gamma-glutamyl transferase (GGT) using commercially available kits and reagents. The serostatus of cows against A. marginale was determined using a commercially available cellular enzyme-linked immunosorbent assay kit according to the manufacturer's recommendations. Significant differences in serum biochemical values between seropositive and seronegative groups were evaluated using independent Student's t-test. Possible associations between the serostatus of the cows and different biochemical parameters were evaluated using univariate followed by multivariate logistic regression analyses. Results: There was a statistically significant increase (p≤0.05) in values of total protein, BHB, LDH, and AST in seropositive cows compared to seronegative cows while a non-significant increase in values of ALP, ALT, and GGT was detected in seropositive cows. A strong correlation (R=0.69) between serum levels of BHB, LDH, and AST and seropositivity against A. marginale was detected. Conclusion: There is evidence of a possible association between A. marginale infection and liver damage/hepatic fatty degeneration in dairy cows. Further studies, however, are required to elucidate the exact pathophysiological mechanisms of this relationship.

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19 EFFECTS OF ESTRADIOL OR GnRH ON FOLLICULAR WAVE EMERGENCE AND PREGNANCY RATES FOR A CIDR-BASED TIMED AI PROTOCOL IN REPEAT BREEDER DAIRY COWS

Reproduction Fertility and Development ◽

10.1071/rdv19n1ab19 ◽

2007 ◽

Vol 19 (1) ◽

pp. 128

Author(s):

G. H. Suh ◽

U. H. Kim ◽

T. Y. Hur ◽

S. J. Kang ◽

K. S. Baek ◽

...

Keyword(s):

Dairy Cows ◽

Follicular Development ◽

Economic Loss ◽

Estradiol Benzoate ◽

Pregnancy Rates ◽

Corpora Lutea ◽

Chi Square ◽

Follicular Wave ◽

Array Transducer ◽

Repeat Breeder

Repeat breeding of a high yielding cow causes enormous economic loss to the dairy producers. In this study, we examined the effect of estradiol benzoate (EB) or GnRH on follicular wave emergence and follicular development, and on pregnancy rates for a CIDR-based timed AI (TAI) protocol in repeat breeder dairy cows. Repeat breeder cows received a CIDR device (InterAg, Hamilton, New Zealand), with an injection of 1 mg EB (SY Esrone�; Samyang, Seoul, Korea) plus 50 mg progesterone (P4; SY Ovaron�; Samyang) (EB + P4 group, n = 36) or with an injection of 250 �g Gonadorelin (GnRH, Fertagyl�; Intervet, Boxmeer, The Netherlands) (GnRH group, n = 35) at the beginning of the experiment (Day 0). On Day 7, CIDR devices were removed and all cows received PGF2� (Lutalyse�; Pharmacia & Upjohn, Puurs, Belgium). Thereafter, cows in the EB + P4 group received an injection of 1 mg EB on Day 8, and TAI 30 h later; those in the GnRH group received an injection of 250 �g GnRH on Day 9, and TAI 19 h later. In a subset of cows for both groups (15 cows from each group), the ovaries of the cows were examined by transrectal ultrasonography (Sonoace 600 with 7.5 MHz linear-array transducer; Medison Co., Seoul, Korea) every 24 h from Days 0 to 8, on Day 10, and on Day 13 for the EB + P4 group, and every 24 h from Days 0 to 9, on Day 11, and on Day 14 for the GnRH group, to observe changes in ovarian structures (follicles and corpora lutea). Pregnancy was diagnosed 35 to 60 days after TAI, using both ultrasonography and rectal palpation. Data were analyzed using Fisher's exact, Student's t-, and chi-square tests. Follicular wave emergence occurred within 7 days in 12/15 EB + P4-treated and 13/15 GnRH-treated cows (P > 0.05). The mean diameters of preovulatory follicles were smaller (P < 0.05) in the EB + P4 group (12.1 � 0.1 mm) than in the GnRH group (13.8 � 0.1 mm). However, the proportion of cows with synchronized ovulation and pregnancy rates to TAI did not differ (P > 0.05) between the EB + P4 (11/15 and 19.4%, respectively) and the GnRH (13/15 and 34.3%, respectively) groups. In conclusion, treatment with either EB or GnRH for a CIDR-based TAI protocol in repeat breeder dairy cows results in synchronous follicular wave emergence, follicular development, and ovulation, and thus similar pregnancy rates to TAI.

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Characterisation and Pattern of Culling in Holstein Dairy Cows in Torbat-E-Jam Area, Northeast of Iran

Journal of Agricultural Studies ◽

10.5296/jas.v1i2.3957 ◽

2013 ◽

Vol 1 (2) ◽

pp. 151 ◽

Cited By ~ 1

Author(s):

Massod Didarkhah ◽

Mohsen Danesh Mesgaran ◽

Elias Ibrahimi Khorram Abadi ◽

Fereshteh Jamili ◽

Seyed Mohammad Hosseini

Keyword(s):

Dairy Cows ◽

Dairy Herd ◽

Economic Loss ◽

Dairy Herds ◽

Reproductive Disorders ◽

Lactating Cows ◽

Milk Fever ◽

Common Infectious Disease ◽

Infection Disease ◽

Digestive Disorders

To describe proportion and pattern of culling in commercial dairy herds of Torbat-e-Jam region in northeastern of Iran, this survey was carried out over a period of 15 years from 1997 to 2011 in industrialized dairy herd. In total, the data of 4149 lactating cows have been used in this study. Overall, 415 (09.0%) cows were culled. The involuntary culling were mainly from infertility or reproductive disorders (4%), followed by metabolic and digestive disorders (3%), infection disease (1%) and lameness (1%). Infertility accounted for 55% of disposals in culled cows. Milk fever was the most important reason (26%) for culling due to metabolic and digestive track disorders. Pneumonia was the most common infectious disease that contributed to culling, and on average, it was responsible for 43% of disposals. Subacute mastitis accunted 88% of infection disease disposal. Bone fracture, vagal syndrome and foreign body accunted 19%, 31% and 38% of other disorders disposal, respectively. Aproximately, 13% of the recorded cullings were for unknown reasons. High proportion of infertility or reproductive culling specially poor fertility should be considered as an important economic loss and precaution measures are necessary to reduce this loss.

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Physiological Comparison of Some Serum Biochemistry between Lactating and Non-Lactating Dairy Cows in Selected Dairy Farms of Dhaka District of Bangladesh

International Journal of Current Science Research and Review ◽

10.47191/ijcsrr/v4-i1-09 ◽

2021 ◽

Vol 04 (01) ◽

Author(s):

Sajibul Hasan ◽

Keyword(s):

Dairy Cows ◽

Total Cholesterol ◽

Total Protein ◽

Biochemical Parameters ◽

Laboratory Data ◽

Dairy Farms ◽

Serum Biochemistry ◽

Lactating Cows ◽

Lactating Dairy Cows ◽

Calcium And Phosphorus

The present study was carried out to investigate the physiological comparison of some serum biochemistry between lactating and non-lactating dairy cows in selected dairy farms of Dhaka district of Bangladesh. The aim of the current study was to evaluate the serum parameters like glucose, total protein, total cholesterol, calcium and phosphorus concentration and these parameters were measured by the serum analyzer for lactating (n=50) and non-lactating (n=50) dairy cattle which were selected from various selected dairy farms of Dhaka district. The results (mean ± standard deviation) of the study showed that among the serum biochemical parameters total protein (84.2±1.18 gm/l) and total cholesterol (156.36± 3.41 mg/dl) level in non-lactating cows were higher than the value of lactating cows as (80.14±2.31 gm/l) and (138.21± 1.21 mg/dl). On the other hand, serum glucose, calcium and phosphorus level in lactating cows (68.12± 2.34 mg/dl), (12.06±3.21 mg/dl) and (8.30±0.20 mg/dl) respectively were found higher than the non-lactating cows (60.32 ±1.42 mg/dl), (10.48±5.60 mg/dl) and (6.31 ±0.12 mg/dl). In case of parity-wise biochemical parameters were insignificant variation was found. It may be concluded that biochemical parameters variation present between lactating cow and non-lactating cow is important for interpretation of laboratory data and also for physiological diagnosis.

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