scholarly journals Antimicrobial sensitivity patterns of salmonella typhi in children

2016 ◽  
Vol 15 (3) ◽  
pp. 416-418
Author(s):  
Md Khoyber Ali ◽  
Shahin Sultana
Keyword(s):  
Blood Culture ◽  
Nalidixic Acid ◽  
Medical Science ◽  
Antibiotic Sensitivity ◽  
Public Health Problem ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
Sensitivity Testing ◽  
Antimicrobial Sensitivity ◽  
Sensitivity Pattern

Background: Enteric fever is an important public health problem in developing countries including Bangladesh. A changing antibiotic sensitivity pattern of Salmonella typhi and emergence of resistance has increased to a great concern. Objective: Aim of the study was to investigate the antibiotic sensitivity pattern of Salmonella typhi. Methods: A total of 181 Salmonella typhi samples from 5 to 15 years age group were collected from blood culture during the period of October to December 2014 from IBN SINA Hospital, Dhaka, Bangladesh. Specimens from the blood culture were identified by standard procedures as needed. Antimicrobial susceptibility testing was performed by disk diffusion method according to ‘The Clinical Laboratory Standard Institute’ guidelines. Results: Among the tested antibiotics, S. typhi was susceptible to ceftriaxone 100%, followed by cefixime and gentamicin 99.4%, ciprofloxacin 98.6%, cotrimoxazole 88.9%, azithromycin 88.4 % and least susceptible antibiotic was Ampicillin 62.5% and nalidixic acid 5%. Conclusion: The antimicrobial sensitivity testing showed that the Salmonella typhi were highly sensitive (>88%) to most of the drugs used in this study, whereas nalidixic acid showed only 5% sensitivity. So this study indicates that ceftriaxone, cefixime, gentamicin and ciprofloxacin can be used as a first line therapy and nalidixic acid should be avoided for treatment.Bangladesh Journal of Medical Science Vol.15(3) 2016 p.416-418

scholarly journals Is Antimicrobial Resistance Pattern of Enteric Fever Changing in Kathmandu Valley?

2013 ◽  
Vol 32 (3) ◽  
pp. 221-228
Author(s):  
Ganesh Kumar Rai ◽  
Subhana Karki ◽  
Bina Prajapati
Keyword(s):  
Antimicrobial Resistance ◽  
Nalidixic Acid ◽  
Enteric Fever ◽  
Public Health Problem ◽  
Salmonella Typhi ◽  
Resistance Pattern ◽  
Kathmandu Valley ◽  
Sensitivity Testing ◽  
Antimicrobial Sensitivity ◽  
Sensitivity Pattern

Introduction: Enteric fever is a public health problem in developing countries including Nepal. Antimicrobial sensitivity pattern of Salmonella sp causing enteric fever is changing over time and also differs according to geographical location. Periodic surveillance of antimicrobial resistance of Salmonella sp is mandatory for management of enteric fever rationally. The objective of this study was to analyze the resistance pattern of Salmonella sp to commonly used antimicrobials. Materials and Methods: This was a retrospective study undertaken reviewing the records of blood isolates of Salmonella sp over one year period at Kanti Children’s Hospital, Nepal. Antimicrobial sensitivity testing was done following Kirby Bauer’s disk diffusion technique using Muller Hinton agar. Results: Salmonella typhi was found to be 100% resistance to ampicillin and resistance to nalidixic acid and ciprofloxacin was also high. The resistance to chloramphenicol and ofloxacin was low. No resistance was found to amoxicillin, ceftriaxone, cefotaxime and amikacin. However, among the sensitive isolates of Salmonella typhi intermediate sensitivity to ceftriaxone and ceftazidime was reported to be high. Among the isolates of S paratyphi A, the resistance to nalidixic acid was 100% followed by 75% to ciprofloxacin. No resistance was found to chloramphenicol, ceftriaxone, cefixime, amikacin and low resistance to amoxicillin and ofloxacin. Among the sensitive isolates of S paratyphi A, majority of them were only moderately sensitive to cefotaxime and ceftazidime and about 1/3 of the organisms had only intermediate sensitivity to ceftriaxone. Conclusiion: Commonly used parenteral third generation cephalosporins, first line drugs like chloramphenicol and amoxicillin and ofloxacin among fluoroquinolones were found to be effective in vitro in treating enteric fever. However, Salmonella typhi was found to be highly resistant to the most frequently used drugs like ciprofloxacin and cefixime and Salmonella paratyphi A to ciprofloxacin. A comparative chart of antimicrobial sensitivity of enteric fever in children over 10 year period from different hospitals of Kathmandu valley is also presented in the study. DOI: http://dx.doi.org/10.3126/jnps.v32i3.6625 J. Nepal Paediatr. SocVol.32(3) 2012 221-228

scholarly journals Evaluation of Pathogens Causing Urinary Tract Infection and Their Sensitivity Pattern

2022 ◽  
Vol 8 (1) ◽  
pp. 31-37
Author(s):  
Vijay Kumar ◽  
Swayambhu Shubham ◽  
Satyendra Narayan Singh
Keyword(s):  
Tract Infection ◽  
Antibiotic Sensitivity ◽  
Public Health Problem ◽  
Diffusion Method ◽  
Major Public Health Problem ◽  
Sensitivity Testing ◽  
Antimicrobial Susceptibility Pattern ◽  
Antimicrobial Sensitivity ◽  
Sensitivity Pattern ◽  
Improper Use

Background: UTI constitute a major public health problem in India accounting 2nd most common infection next to respiratory tract infection. They are responsible for increasing treatment cost and significant morbidity.Aim:-To determine the incidence of UTI, evaluation of pathogens responsible and their antimicrobial susceptibility pattern in the population.Methods:Urine samples were collected from 300 patients attending the OPD Patna medical college, Patna during the period of 18 months (January 2017 to June 2018) Antimicrobial sensitivity testing was done for the bacterial isolates present in the sample by Kirby- Bauer disc diffusion method. Only those samples were taken into consideration which develops count equal to or greater than 1*105CFU/ml as indicated by Kass.Results:Out of 300 samples collected 146 (48.66%)) yielded bacterial growth. Out of 146 culture isolates E.Coli was the most common pathogen followed by klebsiella, CoNS and staphylococcus. Antibiotic sensitivity was performed on all the isolates. It was observed that highest sensitivity was 49.31% to amikacin, gentamycin (45.89%), nitrofurantoin (38.35%) meropenem (27.39%).Conclusions:It was observed that high grade of resistance to ampicillin, cotrimoxazole, ciprofloxacin, cefuroxime, chloramphenicol, cefotaxime, cefazolin, amoxicillin + clavulanic acid and gentamycin is present as a result of misuse or improper use of antibiotic in the community. Hence urine culture is necessary for the diagnostic screening of UTI before the treatment.

scholarly journals Antimicrobial Sensitivity Pattern of Microorganisms Isolated from Vaginal Infections at a Tertiary Hospital in Bangalore, India

2015 ◽  
Vol 3 (1) ◽  
pp. 34-39 ◽  
Author(s):  
Nagalakshmi Narayana-Swamy ◽  
Padmasri Ramalingappa ◽  
Urvashi Bhatara
Keyword(s):  
Low Frequency ◽  
Antibiotic Sensitivity ◽  
Tertiary Hospital ◽  
Vaginal Swab ◽  
Diffusion Method ◽  
Sensitivity Testing ◽  
Vaginal Infections ◽  
Treatment Protocols ◽  
Antimicrobial Sensitivity ◽  
Sensitivity Pattern

Background: The vagina contains dozens of microbiological species in variable quantities and is, therefore, considered a complex environment. Among the microorganisms, bacteria have important repercussions on women’s health. The present study was conducted to elucidate this type of vaginal isolates and their sensitivity towards currently used antibiotics. Methods: This was a retrospective study conducted at the Department of Obstetrics and Gynaecology, Sapthagiri Hospital, Bangalore, India from January 2012 to December 2013. All symptomatic women who had a high vaginal swab taken for culture and sensitivity testing were included in this study. Antibiotic susceptibility was tested using disc diffusion method (modified Kirby-Bauer’s method). The antibiotic sensitivity patterns of isolated microorganisms were studied. Results: Out of 200 patients, 95% had positive vaginal cultures. Fifteen types of microorganisms were isolated. The highest frequency of infection was seen at the age of 20-30 years, followed by 41-50 years and 31-40 years, and a low frequency of infection was observed above 50 years of age. The most prevalent pathogen was Escherichia coli, followed by Streptococcus agalactiae and diphtheroids with equal incidence. Among the antibiotics tested, isolated pathogens were completely resistant to nalidixic acid and highly sensitive to meropenem and imepenem. Conclusion: The high prevalence of gynaecological infections demands that patients with symptoms undergo thorough investigation with cultures and sensitivity essays. Changes in treatment protocols are required to treat vaginal infections effectively.

scholarly journals Antimicrobial Sensitivity Pattern of Salmonella spp. Clinical Isolates in Blood Culture: A Tertiary Center Study

2020 ◽  
Vol 11 (2) ◽  
pp. 134-137
Author(s):  
ABM Shakil Gani ◽  
Ayman Wadud ◽  
Mizanur Rahman ◽  
Sushmita Roy ◽  
Selin Aktari
Keyword(s):  
Blood Culture ◽  
Enteric Fever ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
Resistance Pattern ◽  
Local Resistance ◽  
Salmonella Spp ◽  
Antimicrobial Sensitivity ◽  
Sensitivity Pattern ◽  
Tertiary Center

Objective: Bangladesh is an endemic area for enteric fever. Adequate and timely antimicrobial treatment invariably cures this disease. But resistant strains of Salmonella spp. have made it difficult to treat nowadays. This study will help clinicians in understanding the local resistance pattern of enteric fever and identifying recent changes in the trends of the sensitivity pattern of commonly used antibiotics in Bangladesh. Materials and Methods: This cross-sectional study was conducted during the period from January 2016 to June 2019 in Sheikh Hasina Medical College, Tangail, to determine the antimicrobial sensitivity patterns of Salmonella typhi and paratyphi isolated by blood culture from clinically suspected typhoid fever patients. The blood sample was processed on BACTEC 9050 and isolates obtained from subculture were serotyped. Then antibiotic susceptibility testing was carried out using the disk diffusion method. Results and Discussion: A total of 137 cases were recorded in four years. All cephalosporins were found sensitive in more than 90% of cases. We did not find any resistance to Ceftriaxone in our patients’ group. Moxifloxacin and Levofloxacin showed higher sensitivity than Ciprofloxacin (100% and 96% Vs 90%). Nalidixic acid demonstrated lower sensitivity than previously reported (less than 5%), while Gentamicin showed the highest sensitivity around 100%. Conclusion: Multidrug-resistant (MDR) Salmonella typhi and paratyphi are emerging rapidly. To overcome this global issue, rational use of antibiotics has to be ensured. Physicians should also be aware of the local resistance pattern so that they can treat their patients effectively. J Shaheed Suhrawardy Med Coll, December 2019, Vol.11(2); 134-137

Antibiotic Sensitivity and Resistance Patterns of Salmonella Typhi in Rajshahi Medical College Hospital

2020 ◽  
Vol 33 (2) ◽  
pp. 10-14
Author(s):  
Md Azizul Haque ◽  
Laila Shamima Sharmin ◽  
KM Faisal Alam ◽  
Md Mohimanul Hoque ◽  
M Morsed Zaman Miah ◽  
...  
Keyword(s):  
Blood Culture ◽  
Enteric Fever ◽  
Antibiotic Sensitivity ◽  
Salmonella Typhi ◽  
Medical College Hospital ◽  
Personal Hygiene ◽  
Care Hospital ◽  
College Hospital ◽  
Medical College ◽  
Sensitivity Pattern

Typhoid and paratyphoid fevers, collectively known as enteric fever, is caused by Salmonella enterica subspecies serovars Typhi and Paratyphi A, B and C. Despite this declining global trend, enteric fever is still considered to be a major public health hazard in Bangladesh and other developing countries due to poor sanitation, inadequate food safety measures and poor personal hygiene. In Bangladesh, the incidence of typhoid fever was reported to be 200 episodes per 100,000 person-years during 2003–2004. Multidrug-resistant (resistance to the first-line antimicrobials ampicillin, cotrimoxazole, and chloramphenicol) strains of S. Typhi and S. Paratyphi are on the rise globally and even cases of extensively drug-resistant (XDR) typhoid cases resistant to chloramphenicol, ampicillin, trimethoprim-sulfamethoxazole, third generation cephalosporins and fluoroquinolones are being reported from many corners of the world. This descriptive, observational study was carried out in Rajshahi Medical College Hospital Hospital, Rajshahi, Bangladesh from July 2017 to June 2019. Antibiotic sensitivity pattern of total 76 cases of enteric fever due to Salmonella Typhi were studied. Blood culture was carried out by BACT ALERT-3D, Automated blood culture analyzer from BioMeriuex SA, France Patented FAN Plus method. Based on the minimum inhibitory concentration (MIC), the organism was categorized as sensitive, intermediate, and resistant against the respective antibiotics as per Clinical and Laboratory Standards Institute (CLSI) criteria. We are reporting antibiotic sensitivity and resistant patterns of S. Typhi documented in Rajshahi Medical College Hospital, a large tertiary care hospital in Northern Bangladesh. TAJ 2020; 33(2): 10-14

scholarly journals A study on Nalidixic Acid Resistant Salmonella among the patients attending Shree Birendra Hospital

2012 ◽  
Vol 10 (2) ◽  
pp. 1-3 ◽  
Author(s):  
Raina Chaudhary ◽  
Khagendra Sijapati ◽  
Sunil Kumar Singh
Keyword(s):  
Nalidixic Acid ◽  
Salmonella Enterica ◽  
Enteric Fever ◽  
Brain Heart Infusion ◽  
Public Health Problem ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Diffusion Test ◽  
Sensitivity Pattern

Introduction: Enteric fever is the commonest public health problem in developing countries like Nepal. Multi Drug Resistant Salmonella isolates are in vitro susceptible to Quinolone but exhibited a higher Minimum Inhibitory Concentration in vivo. Such phenomenon can be demonstrated by simple disc diffusion test of Nalidixic Acid which shows resistance. This study is conducted to determine prevalence of Nalidixic Acid Resistant Salmonella with their anti biotic sensitivity pattern at Shree Birendra Hospital, Chauni, kathmandu. Method: Of total 3945 blood samples from the patients suspected to have enteric fever were collected during the period of January 2011 to August 2011. Then it was mixed with Brain Heart Infusion , further processed according to standard methodology and their anti microbial susceptibility was performed by Kirby-Bauer disc diffusion method. Results: From 3945 samples, 280 (7.09%) showed positi ve growth, 114 (40.7%) Salmonella enterica serotype Typhi and 166 (59.2%) Salmonella enterica serotype Paratyphi A. a total of 221 (78.92%) were Nalidixic Acid Resistant Salmonella, all these strains were sensitive to Ciprofloxcin and Ofloxacin in disc diffusion test. Conclusion: Study showed a higher frequency of Nalidixic Acid Resistant Salmonella among the patients. screening of Nalidixic Acid disc diffusion test must be done as routine work for determination of low level resistance of Quinolone so as to decide the drug for the treatment of enteric fever. DOI: http://dx.doi.org/10.3126/mjsbh.v10i2.6453 Medical Journal of Shree Birendra Hospital July-Dec 2011 10(2) 1-3

scholarly journals Prevalent Bacteria and Their Sensitivity and Resistance Pattern to Antibiotics: A Study in Dhaka Medical College Hospital

2017 ◽  
Vol 26 (1) ◽  
pp. 52-64
Author(s):  
Mohammad Zaid Hossain ◽  
Akhtarun Naher ◽  
Pratyay Hasan ◽  
Kazi Tuba E Mozazfia ◽  
Homyra Tasnim ◽  
...  
Keyword(s):  
Nalidixic Acid ◽  
Antibiotic Sensitivity ◽  
Sampling Technique ◽  
Salmonella Typhi ◽  
Medical College Hospital ◽  
Resistance Pattern ◽  
College Hospital ◽  
Medical College ◽  
Sensitivity Pattern ◽  
Changes Over Time

Background and rationale: Antibiotic resistance is a global problem. Many factors are complexly related to the issue in multiple dimensions. Bangladesh is right in the middle of this great calamity, and is seeing the rise in resistant strains of several bacteria. Very sadly, the prevalent malpractice of abusing antibiotics in Bangladesh contributes to add complexity to the danger which may prove to be possibly the greatest threat humans have ever faced. There is much scarcity of medical literature in Bangladesh, on the antibiotic sensitivity pattern and prevalent microorganisms. Moreover, antibiotic sensitivity pattern changes over time and place. Again, most of the studies done in Bangladesh, concentrate on a single disease, pathogen, or specimen. This study attempts to see the prevalent microorganisms and the antibiotic sensitivity pattern in multiple types of specimens collected from Dhaka Medical College Hospital. This study also attempts to establish a way of presentation of the relevant findings which can be used in future to ensure easy comparability and contrasting of findings.Methods: The specimens were collected from the adult patients (age >12 years) admitted in the Internal Medicine ward of Dhaka Medical College Hospital, Dhaka, over a period of 6 months. The sampling technique was consecutive sampling method. Specimens which were culture positive, were only included in the study for analysis. Multiple specimens were taken.Results: S. aureus was 100% sensitive to amikacin, moxifloxacin, imipenem, meropenem, piperacillin+tazobactum combination, vancomycin, doxycycline, tetracycline, tigecycline, nitrofurantoin, azactum, linezolid and 100% resistant to cefixime. Enterobacter was 100% sensitive to penicillin, amikacin, gentamicin, netilmicin, doxycycline, tetracycline, tigecycline and 100% resistant to cefixime, ceftazidime, ceftriaxone, cefepime, cotrimoxazole, levofloxacin, vancomycin. E. coli was 100% sensitive to imipenem, meropenem, vancomycin, tigecycline and 100% resistant to mecillinam, aztreonam. Klebsiella was 100% sensitive to flucloxacillin, colistin, vancomycin, tigecycline, linezolid and 100% resistant to nalidixic acid. Proteus was 100% sensitive to cephradine, cefoxitin, cefixime, ceftazidime, ceftriaxone, cefepime, cotrimoxazole, amikacin, ciprofloxacin, imipenem, meropenem, netilmicin, piperacillin+tazobactum combination, tetracycline, tigecycline, azithromycin, azactum and 100% resistant to doxycycline, tetracycline, chloramphenicol and cefuroxime. Pseudomonas was 100% sensitive only to amikacin, netilmicin, and 100% resistant to cefixime, ceftazidime, ceftriaxone, cefepime, cotrimoxazole, gentamicin, ciprofloxacin, levofloxacin, imipenem, meropenem, doxycline, tetracycline, chloramphenicol. Salmonella typhi was 100% sensitive to amoxicillin, cefoxitin, cefixime, ceftriaxone, cefepime, cotrimoxazole, amikacin, netilmicin, azithromycin, chloramphenicol, azactum and 100% resistant to cephradine, doxycycline, tetracycline, nalidixic acid. MRSA was 100% sensitive to imipenem, vancomycin, teicoplanin, nitrofurantoin, linezolid and 100% resistant to cefpirome, cefoxitin, ceftazidime, cotrimoxazole, clindamycin, gentamicin, ciprofloxacin, netilmicin, tetracycline, clarithromycin. Acinetobacter was 100% sensitive to penicillin, cefuroxime, colistin, piperacillin+tazobactum combination, tigecycline, chloramphenicol and 100% resistant to cefixime, nalidixic acid. Citrobacter freundii was 100% sensitive to ceftazidime, ceftriaxone, cotrimoxazole, amikacin, gentamicin, ciprofloxacin, levofloxacin, imipenem, meropenem, netilmicin, nalidixic acid and 100% resistant to ampicillin, cefixime, nitrofurantoin.Conclusion: More and more antibiotics are becoming ineffective due to emergence of resistance. Serious actions should be taken. Awareness should be raised from the policy maker level to the physicians and patients.J Dhaka Medical College, Vol. 26, No.1, April, 2017, Page 52-64

scholarly journals Pattern of Antibiotic Sensitivity in Enteric Fever: A Tertiary Care Hospital Experience

2014 ◽  
Vol 15 (2) ◽  
pp. 122-124 ◽  
Author(s):  
Samira Rahat Afroze ◽  
Muhammad Abdur Rahim ◽  
Md. Mehedi Hasan ◽  
Farhana Afroz ◽  
Hasna Fahmima Haque ◽  
...  
Keyword(s):  
Blood Culture ◽  
Nalidixic Acid ◽  
Enteric Fever ◽  
Tertiary Care ◽  
Antibiotic Sensitivity ◽  
Cross Sectional Study ◽  
Care Hospital ◽  
Cross Sectional ◽  
Sensitivity Pattern ◽  
Drug Resistant Strains

Objectives: To describe the antibiotic sensitivity pattern ofSalmonella typhi and Salmonella paratyphi from blood culture specimens. Methods: This cross-sectional study was done in the Department of Medicine, BIRDEM from July 2009 to June 2012. Standard laboratory and microbiological procedures were followed for blood culture and antibiotic sensitivity tests. Results: Among the 97 blood culture positive samples, S. typhi was 71 (73.2%) and S. paratyphi was 26 (26.8%). Multi-drug resistant strains of S. typhi and S. paratyphi were 23 (32.4%) and 3 (11.5%) cases respectively. Azithromycin, nalidixic acid, ciprofloxacin, levofloxacin and amoxicillin resistance was also found in a good number of cases (S. typhi and S. paratyphi: 71.8% and 57.7%, 42.3% and 30.8%, 38% and 34.6%, 38% and 26.9% and 38% and 26.9% cases respectively). Nineteen (31.1%) of the 61ciprofloxacin sensitive organisms were resistant to nalidixic acid. Ceftriaxone was sensitive in 100% of S. typhi and S. paratyphi. Cefixim, ciprofloxacin, levofloxacin, imipenem were among the most common sensitive antibiotics (S. typhi and S. paratyphi: 83.1% and 73.1%, 62% and 65.4%, 53.5% and 65.4%, 76.1% and 65.4% cases respectively). Conclusion: Ceftriaxone was the most sensitive antibiotic for treating enteric fever followed by cefixim, imipenem and ciprofloxacin. However, in suspected cases of enteric fever, blood culture should be requested before prescribing antibiotic.DOI: http://dx.doi.org/10.3329/jom.v15i2.20684 J MEDICINE 2014; 15 : 122-124

scholarly journals Phage Types of multidrug resistant Salmonella species in a rural area of Maharashtra, India

1970 ◽  
Vol 11 (1) ◽  
pp. 37-39
Author(s):  
ST Sabiha ◽  
SL Nilekar
Keyword(s):  
Antimicrobial Susceptibility ◽  
Phage Type ◽  
Antibiotic Sensitivity ◽  
Salmonella Typhi ◽  
Multidrug Resistant ◽  
Phage Typing ◽  
Diffusion Method ◽  
Inappropriate Use ◽  
Phage Types ◽  
Sensitivity Pattern

Objective: The aim of the present study is to know Phage typing and antimicrobial susceptibility pattern of Salmonella species. Methods: A total of 32 Salmonella species isolated from stool and blood specimen were included in the study. They were sent for phage typing to Lady Hardinge Medical College, New Delhi. Antimicrobial susceptibility was done by using Kirby Bauer disc diffusion method. Results: Majority of Salmonella typhi belongs to phage type E1 and biotype I. Decreased susceptibility of the isolate was observed to Ampicillin, Cotrimoxazole, Cephalexin and Gentamicin. Majority of the isolates were sensitive to Ciprofloxacin. Conclusion: Predominant phage type was E1. The antibiotic sensitivity pattern is changing and resistant cases are emerging due to inappropriate use of antibiotics. DOI: http://dx.doi.org/10.3329/bjms.v11i1.9821 BJMS 2012; 11(1): 37-39

scholarly journals Antibiotic Sensitivity of Bacteria Causing Urinary Tract Infection

2017 ◽  
Vol 2 (1) ◽  
pp. 13-18
Author(s):  
Rezina Parveen ◽  
Md. Abdullah Yusuf ◽  
Ishrat Sharmin ◽  
Md. Saiful Islam ◽  
Ina Rahim
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Antimicrobial Agents ◽  
Antibiotic Sensitivity ◽  
Diffusion Method ◽  
College Hospital ◽  
Antimicrobial Sensitivity ◽  
Sensitivity Pattern

Background: Urinary tract infection is very common in both male and female.Objectives: The purpose of the present study was to see the antibiotic sensitivity pattern of isolated from urinary tract infected patients.Methodology: This cross sectional study was carried out in Dhaka Medical College Hospital, Dhaka for a period of 12 months. Clinically diagnosed cases of urinary tract infection irrespective of age and sex having pus cells ?5/HPF in the deposits of centrifuged urine were selected as study population. Data regarding organisms causing UTI and their antibiotic sensitivity patterns were collected. For urine culture the urine samples were inoculated on HiCrome UTI agar, CLED agar, 5% sheep blood agar and MacConkey’s agar media with a calibrated loop having diameter of 1.45 mm which contains 0.001 ml of urine. The inoculation at 37o C for 24 hours and CFU count of 105/ml of urine were considered positive for UTI. Identification of bacteria was done by standard biochemical techniques and their distinct colony characteristics. All the isolated organisms were tested for antimicrobial sensitivity against different antimicrobial agents by disc diffusion method on Mueller-Hinton agar plates.Result: Diagnosis of bacteria causing UTI with their sensitivity to different antibiotics was performed with a total of 300 samples from both male (38.66%) and female (61.33%) of different age groups. Among 300 samples 107 strains were isolated. Out of 107 identified strains, 95(31.67%) samples showed single growth and 6(2%) samples showed mixed growth. Escherichia coli (64.49%) was found to be the predominant organism. Regarding antimicrobial sensitivity pattern Esch. coli showed 98.55 to 63.77% sensitivity to imipenem, amikacin, ceftazidime and nitrofurantoin. Other isolated organisms showed 50 to 100% sensitivity to ceftazidime, amikacin, imipenem except Klebsiella, Pseudomonas and enterococci spp. which showed 40% and less sensitivity.Conclusion: In conclusion Escherichia coli is the most commonly isolated bacteria which is highly sensitive to imipenem.Bangladesh J Infect Dis 2015;2(1):13-18

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