Cytotoxic, thrombolytic, membrane stabilizing and anti-oxidant activities of Hygrophila schulli
<p class="Abstract">The crude methanol extract of Hygrophila schulli and its petroleum ether, chloroform, ethyl acetate and aqueous soluble Kupchan partitionates were investigated for in vitro cytotoxic, thrombolytic, membrane stabilizing and anti-oxidant activities. In the brine shrimp lethality bioassay, the petroleum ether soluble extract of H. schulli showed significant cytotoxicity (LC<sub>50</sub> = 0.1 µg/mL and LC<sub>90</sub> = 15 µg/mL) as compared to vincristine sulfate (LC<sub>50</sub> = 0.4 µg/mL and LC<sub>90</sub> = 9 µg/mL). Among all the partitionates, chloroform soluble fraction demonstrated the highest thrombolytic activity with 10.5% clot lyses. Moreover, in hypotonic- and heat-induced conditions, the chloroform soluble extractive inhibited hemolysis of human erythrocyte by 113.7% and 14.3%, respectively as compared to 71.9% and 42.2% demonstrated by standard acetylsalicylic acid. On the other hand, in anti-oxidant activity test, chloroform soluble fraction revealed mild antioxidant activity (IC<sub>50</sub> = 195.1 µg/mL) as compared to standard tert-butyl-1-hydroxytoluene (IC<sub>50</sub> = 27.5 µg/mL).</p><p> </p>