scholarly journals Phytochemical and Biological Investigations of Eurya acuminata (Theaceae)

2017 ◽  
Vol 15 (2) ◽  
pp. 151-154 ◽  
Author(s):  
Tahmid Faisal ◽  
Monira Ahsan ◽  
Jakir Ahmed Choudhury ◽  
ATM Zafrul Azam
Keyword(s):  
Brine Shrimp ◽  
High Field ◽  
Methanol Extract ◽  
Methanolic Extract ◽  
Antimicrobial Properties ◽  
Spectroscopic Studies ◽  
Vincristine Sulfate ◽  
Thrombolytic Activity ◽  
Aqueous Fraction ◽  
Lc50 Value

The methanol extract of the powdered leaf of Eurya acuminata was investigated for isolation of secondary metabolites and two compounds were obtained by using VLC, column chromatography and TLC. The compounds were identified as phytol (1) and ?-sitosterol by extensive spectroscopic studies, including high field NMR analyses as well as co-TLC with authentic samples. The methanol extract of leaf of E. acuminata and its organic and aqueous soluble partitioning materials were evaluated for cytotoxic, thrombolytic and antimicrobial properties. In the cytotoxicity study the aqueous fraction of crude methanolic extract showed significant lethality towards brine shrimp having LC50 value 8.821 ?g/ml as compared to standard vincristine sulfate (0.404 ?g/ml). In the study for thrombolytic property, different extract of E. acuminata exhibited various thrombolytic activity ranging from 13.66 to 31.89 % as compared to standard streptokinase (46.51 %). No antimicrobial activity was observed from leaf extracts.Dhaka Univ. J. Pharm. Sci. 15(2): 151-154, 2016 (December)

scholarly journals In Vitro Anticancer MCF-7, Anti-Inflammatory, and Brine Shrimp Lethality Assay (BSLA) and GC-MS Analysis of Whole Plant Butanol Fraction of Rheum ribes (WBFRR)

2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Jahangir Khan Achakzai ◽  
Muhammad Anwar Panezai ◽  
Muhammad Ayub Kakar ◽  
Abdul Manan Kakar ◽  
Shahabuddin Kakar ◽  
...  
Keyword(s):  
Cell Line ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Aqueous Fraction ◽  
Brine Shrimp Lethality ◽  
Butanol Fraction ◽  
Whole Plant ◽  
Brine Shrimp Lethality Assay ◽  
Anti Inflammatory ◽  
Mcf 7

In this study, GC-MS analysis has shown that whole plant butanol fraction of rheum ribes (WBFRR) comprises of 21 compounds which exhibited anticancer (MCF-7) activity having IC50 value of 36.01± 0.26. MTT assay (MCF-7), Oxidative Burst assay using chemiluminescence technique, and B-Hatching techniques were the methods used for anticancer MCF-7, anti-inflammatory, and Brine Shrimp Lethality Assay (BSLA). GC-MS was used for structural elucidation. Whole plant methanol extract of rheum ribes (WMERR), whole plant n-hexane fraction of rheum ribes (WHFRR), and whole plant aqueous fraction of rheum ribes (WAFRR) were inactive against anticancer (MCF-7) cell line. Whole plant methanol extract of rheum ribes (WMERR), whole plant aqueous fraction of rheum ribes (WAFRR) and whole plant butanol fraction of rheum ribes (WBFRR) showed anti-inflammatory activity on ROS having IC50 value of 23.2±1.9, 24.2±2.7 and 12.0±0.6. Whole plant butanol fraction of rheum ribes (WBFRR) showed Brine Shrimp Lethality with LD50 693.302 while whole plant methanol extract of rheum ribes (WMERR) and whole plant aqueous fraction of rheum ribes (WAFRR) showed high lethality at highest concentration. This study revealed that whole plant butanol fraction of rheum ribes (WBFRR) exhibited significant anticancer (MCF-7) activity. In the near future, the constituent of whole plant butanol fraction of rheum ribes (WBFRR) can be the alternative drug against MCF-7 cell line with least toxicity and side effects.

scholarly journals Thrombolytic and Cytotoxic Activities of Terminalia bellerica Roxb.

2015 ◽  
Vol 16 (2) ◽  
pp. 131-135
Author(s):  
M Sekendar Ali ◽  
Kazi Omar Faruq ◽  
Atiqul Islam ◽  
AMM Nurullah ◽  
Kazi Ashfak Ahmed Chowdhury ◽  
...  
Keyword(s):  
Brine Shrimp ◽  
Thrombolytic Agent ◽  
Methanolic Extract ◽  
Pharmaceutical Journal ◽  
Cytotoxic Activities ◽  
Cytotoxic Potential ◽  
Thrombolytic Activity ◽  
Brine Shrimp Lethality ◽  
Terminalia Bellerica ◽  
Thrombolytic Effect

The present study was designed to investigate the thrombolytic activity and cytotoxic potential of the methanolic extract of bark of Terminalia bellerica Roxb. The cytotoxicity was assessed with the brine shrimp lethality bioassay and thrombolytic effect with human blood. The brine shrimp lethality bioassay was used to evaluate cytotoxicity (LC50 = 3.21 ?g/ml) compared to vincristine of sulphate (LC50 = 0.512 ?g /ml). It was also evaluated as thrombolytic agent as compared to streptokinase. It has significant thrombolytic activity (32.95%) compared to standard streptokinase (70%). DOI: http://dx.doi.org/10.3329/bpj.v16i2.22293 Bangladesh Pharmaceutical Journal 16(2): 131-135, 2013

scholarly journals Preliminary Cytotoxicity and Antimicrobial Investigation of Anthocaphalus chinensis

2011 ◽  
Vol 3 (3) ◽  
pp. 689-692 ◽  
Author(s):  
M. A. Hossain ◽  
M. Z. Sultan ◽  
A. M. S. Chowdhury ◽  
C. M. Hasan ◽  
M. A. Rashid
Keyword(s):  
Carbon Tetrachloride ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Methanolic Extract ◽  
Stem Bark ◽  
Microbiological Investigation ◽  
Brine Shrimp Lethality ◽  
Inhibition Of Growth ◽  
Test Organisms ◽  
Soluble Fractions

The methanol extract of the stem bark of Anthocephalus chinensis as well as its n-hexane, carbon tetrachloride, chloroform and aqueous soluble fractions were subjected to brine shrimp lethality bioassay and microbiological investigation. In the brine shrimp lethality bioassay, the aqueous soluble partitionate of the methanolic extract revealed the highest cytotoxicity having LC50 of 1.19 mg/ml while in case of antimicrobial screening, the chloroform soluble materials demonstrated moderate inhibition of growth of test organisms. Keywords: Anthocephalus chinensis; Rubiaceae; Cytotoxicity; Antimicrobial. © 2011 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved. doi: 10.3329/jsr.v3i3.7529               J. Sci. Res. 3 (3), 689-692 (2011)

scholarly journals In-vitro cytotoxicity and antioxidant property evaluation from methanolic extract of Cuscuta Reflexa flowers

2015 ◽  
Vol 1 (2) ◽  
pp. 285-291
Author(s):  
Md Hossan Sakib ◽  
Mohammad Shahadat Hossain ◽  
Muhammad Sazzad Hossain ◽  
Asif Al Mahmood ◽  
Md Yasin Sarkar ◽  
...  
Keyword(s):  
Brine Shrimp ◽  
Methanolic Extract ◽  
In Vitro Cytotoxicity ◽  
Standard Drug ◽  
Brine Shrimp Lethality ◽  
Cuscuta Reflexa ◽  
Lc50 Value ◽  
Vincristine Sulphate ◽  
Anti Oxidant

This investigation is made upon the plant Cuscuta reflexa, the flowers of it, to find out its Cytotoxicity property. The anti-oxidant property of this plant part was investigated using methanol extraction. Methanolic extract of Cuscuta reflexa.Was used to evaluate its cytotoxicity in Brine shrimp lethality bioassay where vincristine sulphate was used as standard drug. In Brine shrimp lethality bioassay, LC50 value of the extract was 36.72?g/ml and vincristine sulphate served as the positive control showed LC50 value 10.51?g/ml. So, compared to vincristine sulphate, it is evident that the methanol extract of flowers of Cuscuta reflexa was cytotoxic. In case of anti-oxidant the scavenging power (IC50) of DPPH radical was 29.26, 17.07, 18.29, 19.55 and 54.87?g/ml respectively.Asian J. Med. Biol. Res. June 2015, 1(2): 285-291

scholarly journals Screening of antimicrobial potential of methanolic, acetone and quencher extracts from Cladonia rangiformis Hoffm. and Cladonia pocillum Ach.

2021 ◽  
Author(s):  
Essghaier Badiaa ◽  
Mendili Mohamed ◽  
Khadri Ayda
Keyword(s):  
Methanol Extract ◽  
Methanolic Extract ◽  
Antimicrobial Properties ◽  
Inhibition Zone ◽  
Lysozyme Activity ◽  
Gram Negative Bacteria ◽  
Destructive Effect ◽  
E Coli ◽  
Fungal Hyphae ◽  
Antibacterial And Antifungal

This study compared the efficacy of two species of lichen located in Tunisia belonging to <i>Cladonia rangiformis</i> and <i>Cladonia pocillum</i> species. The antibacterial and antifungal potentials of methanol, acetone and quencher extracts of <i>C. rangiformis</i> and <i>C. pocillum</i> and the lysozyme activity of both methanol extracts were investigated. The results showed that the examined extracts had antimicrobial properties against gram-positive and gram-negative bacteria and anti-Candida properties and that they also limited the spore germination of Penicillium and Aspergillus. Further results showed that the largest diameter of the inhibition zone was obtained by the methanolic extract of <i>C. pocillum</i> with 31 mm and 27.5 mm against <i>E. cloacae</i> and <i>E. coli</i>, respectively. MIC values of bactericidal and fungicidal activities of both Cladonia extracts ranged from 0.25 mg/mL to 2 mg/mL. <i>C. pocillum</i> possess superior lysozyme activity against Staphylococcus aureus and Enterococcus faecalis. Furthermore, the methanol extract of both Cladonia showed a remarkable destructive effect on the morphology of fungal hyphae.

scholarly journals Sedative and anxiolytic effects of methanolic extract from the leaves of Passiflora actinia

2006 ◽  
Vol 49 (4) ◽  
pp. 565-573 ◽  
Author(s):  
Kely Cristina dos Santos ◽  
Stella Maris Tessaro Figura Kurtz ◽  
Simony Davet Müller ◽  
Maique Weber Biavatti ◽  
Rúbia Maria Monteiro Weffort de Oliveira ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Chromatographic Analysis ◽  
Methanol Extract ◽  
Elevated Plus Maze ◽  
Methanolic Extract ◽  
Folk Medicine ◽  
Field Tests ◽  
Aqueous Fraction ◽  
Chromatography Analysis ◽  
Plus Maze

Leaves from several Passiflora species are largely employed in the Brazilian folk medicine as anxiolytic and sedative. In this work the anxiolytic, sedative-like properties and liquid chromatography analysis of methanolic extract of Passiflora actinia were evaluated. The methanol extract and all of its fractions presented significant sedative-like effect in elevated plus-maze and open field tests. Only the aqueous fraction of the methanol extract showed selective anxiolytic activity (30 mg/kg). Chromatographic analysis of the active fractions showed the presence of isovitexin and absence of the classical Passiflora beta-carboline alkaloids or flavonoids such as vitexin, rutin, swertisin, hesperidin and orientin. The tincture obtained from P. actinia leaves presented 0.27 mg/ml of isovitexin and absence of vitexin.

scholarly journals Antioxidant, Thrombolytic and Cytotoxic Activities of Picrasma javanica

2012 ◽  
Vol 11 (1) ◽  
pp. 71-74 ◽  
Author(s):  
Tasnuva Sharmin ◽  
Farhana Islam ◽  
Mohammad Abul Kaisar ◽  
Md Gias Uddin ◽  
Mohammad A Rashid
Keyword(s):  
Antioxidant Activity ◽  
Carbon Tetrachloride ◽  
Total Phenolic Content ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Thrombolytic Activity ◽  
Total Antioxidant ◽  
Soluble Fractions

The methanol extract of leaf of Picrasma javanica as well as its n-hexane, carbon tetrachloride,chloroform and aqueous soluble partitionates were subjected to screening for antioxidant and thrombolytic activities and brine shrimp lethality. The antioxidant potential was evaluated by DPPH, Folin-Ciocalteau reagent and phosphomolybdenum total antioxidant assays using butylatedhydroxytolune (BHT) and ascorbic acid as standards. All fractions showed moderate to strong antioxidant activity, of which the chloroform and aqueous soluble fractions demonstrated the highest activity with the IC50 value of 14.59 ± 0.73 ?g/ml and 18.6 ± 0.15 ?g/ml, respectively. The  total phenolic content of the chloroform and aqueous soluble fractions was 10.4 ± 0.64 and 5.15 ± 0.22 mg of GAE/g of extractive, respectively. Thus, a positive correlation was found between the total phenolic content and total antioxidant activity of P. javanica. The general toxicity was determined by brine shrimp lethality bioassay where the crude extract (LC50 1.04 ± 0.31 ?g/ml) and its n-hexane (LC50 1.28 ± 0.45?g/ml) soluble partitionate demonstrated the  presence of considerable bioactive principles. Mild to moderate thrombolytic activity was discerned by the methanol extract of leaf of P. javanica and its different fractions. During assay for thrombolytic activity, the carbon tetrachloride soluble materials revealed 34.165±1.57 % of clot lysis while standard streptokinase and water, used as positive and negative controls, demonstrated 66.77% and 3.791% lysis of clot, respectively. DOI: http://dx.doi.org/10.3329/dujps.v11i1.12491 Dhaka Univ. J. Pharm. Sci. 11(1): 71-74, 2012 (June)

scholarly journals ANALISIS FITOKIMIA DAN PENENTUAN NILAI LC50 EKSTRAK METANOL DAUN LIWAS

2014 ◽  
Vol 14 (2) ◽  
pp. 125
Author(s):  
Gerry Sumihe ◽  
Max R. J. Runtuwene ◽  
Johnly A. Rorong
Keyword(s):  
Secondary Metabolites ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Artemia Salina ◽  
Phytochemical Analysis ◽  
Test Results ◽  
Brine Shrimp Lethality ◽  
Lc50 Value

ABSTRAK Penentuan nilai LC50 dari ekstrak metanol daun liwas menggunakan metode Brine Shrimp Lethality Test (BSLT) terhadap hewan indikator udang Artemia salina menunjukkan nilai LC50 dari  A. salina bersifat sangat toksik.Nilai LC50 yang didapatkan sebesar 15,696 mg/L. Nilai LC50 ditentukan dengan menggunakan metode SPSS 20.0.Ekstrak metanol daun liwas mengandung kandungan senyawa metabolit sekunder seperti flavonoid, tanin dan saponin sebagai hasil uji fitokimia. Kata kunci: BSLT, Daun Liwas, Metabolit Sekunder,LC50.   PHYTOCHEMICAL ANALYSIS AND DETERMINING LC50 VALUE OF LIWAS LEAVE METHANOL EXTRACT ABSTRACT Determination of LC50 of methanol extract of leaves liwas using Brine Shrimp Lethality Test (BSLT) against animal indicator shrimp Artemia salina showed LC50 values of  A. salina is highly toxic. LC50 values were obtained at 15.696 mg / L. LC50 values determined using SPSS 20.0. Liwas leaf methanol extract contains compounds secondary metabolites such as flavonoids, tannins and saponins as phytochemical test results. Keywords: BSLT, Leaves Liwas, Secondary Metabolites, LC50.

scholarly journals Secondary Metabolites from Sterespermum chelonoides

1970 ◽  
Vol 4 (1) ◽  
Author(s):  
Mohammad R Haque ◽  
Khondaker M Rahman ◽  
Bilkis Begum ◽  
Choudhury M Hasan ◽  
Mohammad A Rashid
Keyword(s):  
Secondary Metabolites ◽  
Key Words ◽  
Full Text ◽  
High Field ◽  
Methanolic Extract ◽  
Stem Bark ◽  
Spectroscopic Studies ◽  
First Report

Five compounds namely, sterekunthal B (1), sterequinone C (2), p-hydroxybenzaldehyde (3), p-hydroxyphenylmethyl ketone (4) and stigmasterol (5) were isolated from methanolic extract of the stem bark of Stereospermum chelonoides. The structures of compounds 1-5 were established by extensive spectroscopic studies, notably high field NMR and MS. This is the first report of occurrence of 1-5 from Stereospermum chelonoides. Key words: Stereospermum chelonoides, Bignoniaceae, Sterekunthal B, Sterequinone C Dhaka Univ. J. Pharm. Sci. Vol.4(1) 2005 The full text is of this article is available at the Dhaka Univ. J. Pharm. Sci. website

scholarly journals Phytochemical assessment of Iphiona aucheri (Bioss.) Anderb. and its cytotoxic, antioxidant and antidiabetic activities

2020 ◽  
Vol 49 (4) ◽  
pp. 913-918
Author(s):  
Muhammad Abdur Rehman Shah ◽  
Rahmat Ali Khan ◽  
Mushtaq Ahmed
Keyword(s):  
Brine Shrimp ◽  
Methanolic Extract ◽  
Antioxidant Activities ◽  
Hexane Fraction ◽  
Total Phenolic ◽  
Aqueous Fraction ◽  
Phenolic Contents ◽  
Total Phenolic Contents ◽  
Total Antioxidant ◽  
Phytochemical Constituents

The presence of phytochemical constituents and estimation of total phenolic contents in Iphiona aucheri (Bioss.) Anderb. stem and assessment of their cytotoxic, total antioxidant and anti-diabetic activity were investigated. All fractions were assessed for phytochemicals, cytotoxic activity, total phenolic contents, antioxidant and anti-diabetic characteristics. Saponins, glycosides, protein and amino acids, carbohydrates and flavonoids were found in aqueous fraction and methanolic extract while they were absent in n-hexane fraction except glycosides and protein. The crude methanolic extract (70.3 ± 1.9%) revealed highest brine shrimp mortalities. Except n-hexane fraction others indicated considerable antioxidant activities via DPPH, ABTS•+ and H2O2 assays. Crude methanolic extract expressed higher inhibition of α-amylase (60.71 ± 0.89%) than glucophage (54.92 ± 0.56%). Non significant correlation of total phenolic contents with percentage antioxidant and anti-diabetic activities of crude methanolic extract and its various fractions was observed in all cases.

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