Antidiarrheal activity of methanolic leaf extract of Rumex vesicarius
<p>This study evaluates the antidiarrheal activity of <em>Rumex vesicarius</em> (leaf) by using in vitro and in vivo assays. Antidiarrheal effect of <em>R. vesicarius</em> was evaluated using castor oil-induced diarrhea model in rat. Weight and volume of the intestinal content were assessed using the enteropooling method. Atropine (3 mg/kg, i.p) was used as positive control. <em>R. Vesicarius</em> at the doses of 200 and 400 mg/kg p.o. significantly retarded castor oil-induced enteropooling and intestinal transit. The gastrointestinal transit rate was studied and <em>R. vesicarius</em> at the doses of 200 and 400 mg/kg significantly inhibited (p<0.001) weight and volume of intestinal content. <em>R. vesicarius</em> caused concentration-dependent (0.01–1 mg/mL) relaxation of spontaneous contractions in isolated rabbit jejunum tissue preparation and inhibited K<sup>+</sup>-80 induced contractions (0.01-5 mg/mL), similar to verapamil, suggestive of calcium channel blockade. Results obtained herein indicate that <em>R. vesicarius</em> may contain effective compounds which can be used as an antidiarrheal agent.</p>