scholarly journals Potential of Mimosa pudica leaf in the treatment of ulcerative colitis in rat

2018 ◽  
Vol 13 (3) ◽  
pp. 241
Author(s):  
Bharati Zaware ◽  
Ritu Gilhotra ◽  
Sanjay Ravindra Chaudhari
Keyword(s):  
Ulcerative Colitis ◽  
Acetic Acid ◽  
Acetic Acid Solution ◽  
Video Clip ◽  
Ethanolic Extract ◽  
Mimosa Pudica ◽  
Ulcer Index ◽  
Colon Tissue ◽  
Standard Drug ◽  
Full Screen

<p class="Abstract">The aim of present investigation was to validate its folk use in the treatment of ulcerative colitis in the rat model. Sprague Dawley rats were divided into nine groups with six animals in each group. The rats received seven days of pretreatment with chloroform, ethyl acetate or ethanolic extract of <em>Mimosa pudica</em>. For induction of ulcerative colitis, rats were administered with 2 mL of 4% acetic acid solution intrarectally. Ulcer index, macroscopical study of the colon, myeloperoxidase and malondialdehyde levels in colon tissue and blood, and histopathology of the colon tissue were studied. Intrarectal instillation of acetic acid caused increased ulcer index, colonic myeloperoxidase and malondialdehyde. Pretreatment with <em>M. pudica</em> ethanolic extract (400 mg/kg) significantly lowered the ulcer index, colonic myeloperoxidase and malondialdehyde as compared with the standard drug prednisolone. The present investigation demonstrates that the ethanol extract of <em>M. pudica</em> leaf is effective in the treatment of ulcerative colitis.</p><p class="Abstract"><strong>Video Clip of Methodology:</strong></p><p class="Abstract">Embedding or block making: 24 sec   <a href="https://www.youtube.com/v/Y1I4tKYMLsE">Full Screen</a>   <a href="https://www.youtube.com/watch?v=Y1I4tKYMLsE">Alternate</a></p><p class="Abstract">Tissue processing: 19 sec   <a href="https://www.youtube.com/v/7nYpxL2qPhg">Full Screen</a>   <a href="https://www.youtube.com/watch?v=7nYpxL2qPhg">Alternate</a></p><p class="Abstract">Section cutting: 33 sec   <a href="https://www.youtube.com/v/_amFL9kNLBw">Full Screen</a>   <a href="https://www.youtube.com/watch?v=_amFL9kNLBw">Alternate</a></p>

scholarly journals The POTENTIAL OF CAESALPINIA CRISTA LEAVES IN THE TREATMENT OF ULCERATIVE COLITIS IN LABORATORY ANIMALS

2018 ◽  
Vol 8 (5) ◽  
pp. 374-381
Author(s):  
Bharati Zaware ◽  
Ritu Gilhotra ◽  
Sanjay Chaudhari
Keyword(s):  
Ulcerative Colitis ◽  
Acetic Acid ◽  
Acetic Acid Solution ◽  
Ethanol Extract ◽  
Experimental Colitis ◽  
Laboratory Animals ◽  
Sprague Dawley ◽  
Ulcer Index ◽  
Standard Drug ◽  
Sprague Dawley Rats

The aim of present investigation was to study the Ulcerative colitis effect of extracts of Caesalpinia crista in acetic acid induced experimental colitis in Sprague Dawley rats. Sprague Dawley rats were divided into nine groups (n=6). The rats were received 7 days pretreatment with chloroform, ethyl acetate, ethanolic extracts of C. crista 200 mg/kg and 400 mg/kg. Ulcerative colitis was induced by intrarectal administration of 1ml of 4% acetic acid solution on 8th day. Prednisolone (2 mg/kg) was used as standard drug administered orally for 3 days. After 48 hrs of colitis induction animals were sacrificed by cervical dislocation to remove colon and distal 5 cm of the colon was dissected. Macroscopical study, Ulcer index of the colon, colonic myeloperoxidase (MPO) and malondialdehyde (MDA) level in colon tissue and blood were studied. Intrarectal instillation of acetic acid caused enhanced ulcer index, myeloperoxidase and malondialdehyde. Ethanol extract of C.crista showed significant effect in lowering ulcer index as well as neutrophil infiltration at a dose of 400 mg/kg in acetic acid induced colitis. The present investigation demonstrates that the ethanol extract of C.crista is of potent therapeutic value in the amelioration of experimental colitis in rat by inhibiting the inflammatory mediator.

scholarly journals A Original Article ANALGESIC ACTIVITY OF BARK AND LEAVES OF FICUS RELIGIOSA L. FROM NEPAL

2020 ◽  
pp. 32-35
Author(s):  
DHARMA RAJ MARASINI ◽  
JITENDRA PANDEY ◽  
LAXMAN PAUDEL SHARMA ◽  
LAXMI PAUDEL ◽  
ROSHNI GYAWALI ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Analgesic Activity ◽  
Test Group ◽  
Ethanolic Extract ◽  
Stem Bark ◽  
Latency Time ◽  
Hot Plate ◽  
Ficus Religiosa ◽  
Standard Drug ◽  
Ethanolic Extracts

Objective: Because of adverse side effects, caused by NSAIDs, tolerance, and dependence induced by opiates, the use of these analgesic agents has not been successful in all cases. Therefore, alternative analgesic drugs from plant sources are the new target now days. The objective of this study was to evaluate the analgesic activity of ethanolic extracts of stem barks and leaves of Ficus religiosa. Methods: The analgesic activity of ethanolic extract of stem barks and leaves was evaluated in the Swiss albino mice model using acetic acid-induced writing response and Eddy’s hot plate method. Analgesic activity was demonstrated with the percentage inhibition of acetic acid induced writings and the percentage increased in latency time of paw licking. The potency of test extracts was compared with standard drug, Diclofenac. Results: Ethanolic extract of leaves and bark of F. religiosa showed potential analgesic activity from both methods. From Eddy’s hot plate model, it was observed that the percentage of increased latency time at 90 min by ethanolic extract of leaves and stem bark was found to be 70.81 % (8.54 min) and 70.78 % (8.53 min) respectively at a dose of 400 mg/kg. Both of these results are statistically significant (p<0.05) as compared to the test group. Furthermore, both of these extracts showed the dose-dependent and time-dependent increased in latency time and these results are compared to that of standard drug Diclofenac. Similarly, ethanolic extract of leaves and stem at 400 mg/kg significantly inhibited the number of writhings induced by acetic acid. The percentage inhibition of writhings by ethanolic extract of leaves at a dose of 400 mg/kg was 68.47 % which was similar to that of standard drug Diclofenac (68.47 %). However, ethanolic extract of bark showed relatively lower percentage inhibition (60.79 %) as compared to leaf extract and standard, but the result was significant as compared to that of the test group (p<0.05). Conclusion: Ethanolic extracts of F. religiosa stem bark and leaf possess both central and peripheral analgesic properties and these effects may be beneficial for the management of pain.

scholarly journals Analgesic and Anti-inflammatory Activities of Ethanolic Extract of Clerodendrum inerme (L.) Gaertn.

2015 ◽  
Vol 17 (1) ◽  
pp. 62-66 ◽  
Author(s):  
Dipa Khanam ◽  
Debashish Deb ◽  
Shrabanti Dev ◽  
Masum Shahriar ◽  
Asish K Das ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Skin Diseases ◽  
Diclofenac Sodium ◽  
Latency Period ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Pharmaceutical Journal ◽  
Edema Formation ◽  
Standard Drug ◽  
Anti Inflammatory

Clerodendrum inerme (L.) Gaertn. (Verbenaceae) is very popular among the traditional practitioners in Bangladesh for the treatment of local pain and inflammation, skin diseases, topical burns etc. However, so far no scientific study has been carried out which may support its uses in traditional medicine. In the present study, we evaluated the possible analgesic and anti-inflammatory activities of the ethanol extract of C. inerme for the first time. Analgesic activity was assessed by using acetic acid-induced writhing and heat-induced pain in mice and anti-inflammatory activity using xylene-induced ear edema in mice at the doses of 250 and 500 mg/kg body weight. The extract significantly (P< 0.05) attenuated the acetic acid-induced writhing with the highest activity being observed at 500 mg/kg b.w. (45.83%) comparable to that of diclofenac sodium (57.64%), the standard drug. A significant dosedependent increase (P< 0.05) of the latency period was also observed in hot plate method. In the xylene-induced inflammation assay, the extract showed significant (P<0.05) and dose dependant inhibitory effect on the edema formation. These findings indicate that the extract has significant analgesic and anti-inflammatory activities which support the folkloric claim of this plant and thus it has a great potential as source of natural products-derived drug. DOI: http://dx.doi.org/10.3329/bpj.v17i1.22317 Bangladesh Pharmaceutical Journal 17(1): 62-66, 2014

scholarly journals Anti-ulcer activity of Cestrum nocturnum leaf in ethanol- and indomethacin-induced ulcer models

2017 ◽  
Vol 12 (3) ◽  
pp. 335
Author(s):  
Uzma Saleem ◽  
Ejaz Ul Haq ◽  
Bashir Ahmad ◽  
Mohammad Saleem
Keyword(s):  
Gastric Juice ◽  
Video Clip ◽  
Gastric Ph ◽  
Control Group ◽  
Antiulcer Activity ◽  
Standard Drug ◽  
Treatment Groups ◽  
Full Screen ◽  
Hexane Extracts ◽  
Dose Dependent Increase

<p class="Abstract">This study was planned to explore the antiulcer activity of the methanolic and <em>n</em>-hexane extracts of <em>Cestrum nocturnum</em> leaf against ethanol- and indomethacin-induced ulcer models in rats. The rats were administered orally vehicle in normal control group, methanolic and <em>n</em>-hexane extracts at doses 300, 500, 700 mg/kg, in treated groups and omeprazole 20 mg/kg, as standard drug. The gastric tissues/contents were examined to determine the ulcer index, antiulcer activity, gastric pH, gastric juice volume and acidity. Both extracts showed dose-dependent increase in antiulcer activity (%) in both ulcer models. Histopathology also supported these results. Gastric pH significantly increased while the gastric juice and acidity significantly decreased in the treatment groups of both ulcer models indicating the anti-secretory effect of extracts. It may be concluded that <em>C. nocturnum</em> protect gastric mucosa by decreasing gastric juice, acidity and increasing the gastric pH.</p><p><strong>Video Clip of Methodology</strong>:</p><p>6 min 58 sec:   <a href="https://www.youtube.com/v/q1ZJszfNJTc">Full Screen</a>   <a href="https://www.youtube.com/watch?v=q1ZJszfNJTc">Alternate</a></p>

scholarly journals Anti-Ulcerative Colitis Activity of the Aqueous Leaves Extract of Khaya Senegalensis in Acetic Acid-Induced Colitis Rat Model

2021 ◽  
Vol 1 (1) ◽  
Author(s):  
Abdul Aziz S.L ◽  
Jimoh A.O ◽  
Yunusa A ◽  
Abdul mumini Y
Keyword(s):  
Ulcerative Colitis ◽  
Acetic Acid ◽  
Rat Model ◽  
Controlled Trial ◽  
Volatile Oil ◽  
Positive Control ◽  
Control Group ◽  
Standard Drug ◽  
Khaya Senegalensis ◽  
Randomised Controlled

intestinal tract mainly colon. The disease is characterized by diarrhoea, blood in the stool, abdominal pain, and weight loss. Khaya senegalensis a plant commonly called dry zone Mahogany has been used as a remedy for several human gastrointestinal ailments. Objectives: To investigate the effect of aqueous leaves extract of Khaya senegalensis in the acetic acid-induced ulcerative colitis rat model. Study design: Preclinical randomised controlled trial. Methods: extraction was done using water, initial phytochemical screening and oral LD50 were determined. Colitis was induced in rats using 4% acetic acid administered intra rectally. The rats were divided into five groups, group 1 received water, groups 2, 3, and 4 received graded doses of leaves extract while group 5 received prednisolone as the positive control. Symptomatic parameters like the daily stool consistency and appearance and histological parameters such as ulcer area, lesson severity score, ulcer index, weight/length ratio and percentage cure were measured. Results; The leaves extract showed presence of alkaloids, tannins, flavonoids, saponins, steroids, volatile oil etc. The oral LD50 was greater than 5000mg/kg and the efficacy of the 400mg/kg extract administered group was not statistically significantly different from the positive control group that received the standard drug prednisolone. Conclusion: It can be concluded that the aqueous leaves extract of Khaya senegalensis possess significant anti-ulcerative colitis activity on acetic acid-induced ulcerative colitis rat models and appears to be safe.

scholarly journals COMPARATIVE EVALUATION OF DIFFERENT DOSES OF VINPOCETINE ALONE AND IN COMBINATION WITH SULFASALAZINE IN EXPERIMENTALLY INDUCED INFLAMMATORY BOWEL DISEASE IN RATS

2017 ◽  
Vol 10 (16) ◽  
pp. 88
Author(s):  
Nishtha Datt ◽  
Rakesh Raman Patyar ◽  
Sazal Patyar
Keyword(s):  
Inflammatory Bowel Disease ◽  
Acetic Acid ◽  
Bowel Disease ◽  
Acetic Acid Solution ◽  
Control Group ◽  
High Dose ◽  
Standard Drug ◽  
Reference Drug ◽  
Group I ◽  
Inflammatory Bowel

  Objective: Inflammatory bowel disease (IBD) is a disorder characterized by chronic inflammation of the gastrointestinal tract and its exact etiopathology is still unclear. Most of the currently available drugs provide the symptomatic improvement, and their long-term use can lead to various unwanted effects also. This study was done to observe the effects of vinpocetine alone and in combination with sulfasalazine on IBD in rats.Methods: Adult Wistar rats of either sex were used (n=36). Experimental colitis was produced by intracolonic administration of acetic acid (10% v/v, 0.20 ml/rat) given per rectally. Rats were divided into six groups (n=6): Group I - normal control (0.9% w/v saline, intracolonic administration + 0.5% w/v carboxymethyl cellulose, i.e., CMC, p.o); Group II - acetic acid (10% v/v, intracolonic administration+0.5% w/v CMC, p.o); Group IIIA - acetic acid + vinpocetine (5 mg/kg, p.o); Group IIIB - acetic acid + vinpocetine (10 mg/kg, p.o); Group IV - acetic acid + sulfasalazine (360 mg/kg, p.o.); Group V - acetic acid + sulfasalazine + vinpocetine (360 mg/kg, p.o. + 5 mg/kg, p.o). The study period was of 15 days in which animals were treated with acetic acid solution on day 1 and treatment was started 4 hrs after the administration of acetic acid till the 14th day. On 15th day, the animals were sacrificed for the investigation of various macroscopic, microscopic, and biochemical parameters.Results: The higher dose of orally administered vinpocetine (10 mg/kg) and combination of sulfasalazine + vinpocetine (360 mg/kg + 5 mg/kg) were found to be the most effective in reducing the severity of mucosal damage which was similar to the reference drug sulfasalazine (360 mg/kg). Both the doses of vinpocetine curtailed the histopathological scores. The combination therapy of sulfasalazine + vinpocetine (360 mg/kg + 5 mg/kg) was equally effective to standard drug but not found to be the most effective treatment. Myeloperoxidase levels were significantly reduced in vinpocetine treated groups as compared to acetic acid control group, while the glutathione levels were increased significantly. Similarly, vinpocetine significantly decreased the malondialdehyde level in the intestinal tissue of the rats with acetic acid induced colitis, and thus the severity of the tissue damage.Conclusion: The results of this study indicated that vinpocetine possesses anti-inflammatory activity and are therapeutically effective in acetic acid induced ulcerative colitis at a dose of 5 mg/kg. More pronounced effects were observed at higher dose, i.e., 10 mg/kg. The combination of sulfasalzine + vinpocetine was also found to be effective as compared to high dose of vinpocetine (10 mg/kg).

scholarly journals Favourable effects of whey protein upon acetic acid-induced ulcerative colitis in rat model

2021 ◽  
Author(s):  
Nurettin TUNC ◽  
abdurahman SAHİN ◽  
Ulvi DEMİREL ◽  
Gokhan ARTAS ◽  
Kazım SAHİN ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Acetic Acid ◽  
Whey Protein ◽  
Histopathological Examination ◽  
Heme Oxygenase 1 ◽  
Related Factor ◽  
Colon Tissue ◽  
Markers Of Inflammation ◽  
Tnf Α ◽  
Cox 2

IntroductionIn the context of the present study, we have aimed to examine the effects of the administration of whey protein through rectal enema to a acetic acid-induced ulcerative colitis in rats on the pathways of nuclear-related factor-2 (Nrf-2), heme oxygenase-1 (HO-1), nuclear factor kappa B (NF-κB), active protein kinase-1 (AP-1), tumour necrotising factor-alpha (TNF-α), and cyclooxygenase-2 (COX-2), IL-6, IL-10.Material and methods28 rats were employed for the trial. Ulcerative colitis was induced through the use of acetic acid. The therapeutic doses of whey protein were administered rectally. Ulcerative colitis was made subject to histopathological examination and protein levels in colon tissue measured with the Western Blot Method.ResultsThe significant increases observed in the levels of AP-1, COX-2, IL-6, IL-10, NF-κβ, and TNF-α as markers of inflammation following the development of ulcerative colitis enjoyed remarkable decreases along with the administration of whey protein (p<0.05). On the other hand, we identified a decrease in the Nrf2-ARE signal pathway and HO-1 protein assuming protective roles on the colon inflammatory response along with the development of ulcerative colitis and an activation of the Nrf2-HO-1 pathway by the whey protein.ConclusionsWhey protein modulates Nrf2/HO-1 and NF-kB pathways, thereby creating a therapeutic effect against colonic inflammation induced by acetic acid (AA) by reason of its anti-inflammatory implications.

scholarly journals Therapeutic Effects of Momordica charantia L. Ethanolic Extract on Acetic Acid-Induced Ulcerative Colitis in Rats

2021 ◽  
Vol 0 (0) ◽  
pp. 119-128
Author(s):  
Dilek Özbeyli ◽  
Ali Şen ◽  
Aslı Aykaç ◽  
Kerem Teralı ◽  
Özlem Çilingir Kaya ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Acetic Acid ◽  
Ethanolic Extract ◽  
Momordica Charantia ◽  
Therapeutic Effects
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