Biological Control of soft rot bacteria of onion in Bangladesh

An investigation was conducted to search antagonistic bacteria as biological control agents of soft rotting bacterial pathogen of onion (Allium cepa L.) in vitro and in storage. Antibacterial activity of previously isolated 91 bacterial isolates was tested in vitro against onion soft rot bacteria Burkholderia cepacia O-15. Two isolates namely, R-15 and E-37 were found antagonistic against onion soft rot bacteria. Isolate R-15 was identified as the genus Bacillus and the isolate E-37 to Lactobacillus sp. Isolate R-15 proved to be a strong antagonist against onion soft rot bacteria was selected for bio-control of onion in storage. That was also effectively reduces the soft rot disease of onion in storage condition. Percentage of disease reduction (PDR) due to treatment with antagonistic bacteria was 72.4% compared to untreated control. It is therefore suggested that this isolate could be exploited as biocontrol agent for onion soft rot in Bangladesh. Bangladesh J. Sci. Ind. Res.56(4), 231-240, 2021

Download Full-text

Botanicals to Control Soft Rot Bacteria of Potato

The Scientific World JOURNAL ◽

10.1100/2012/796472 ◽

2012 ◽

Vol 2012 ◽

pp. 1-6 ◽

Cited By ~ 1

Author(s):

M. M. Rahman ◽

A. A. Khan ◽

M. E. Ali ◽

I. H. Mian ◽

A. M. Akanda ◽

...

Keyword(s):

Plant Extracts ◽

Curcuma Longa ◽

Soft Rot ◽

Storage Condition ◽

Storage Conditions ◽

Leaf Extracts ◽

Corchorus Capsularis ◽

Rot Disease ◽

And Storage

Extracts from eleven different plant species such as jute (Corchorus capsularisL.), cheerota (Swertia chiraitaHam.), chatim (Alstonia scholarisL.), mander (Erythrina variegata), bael (Aegle marmelosL.), marigold (Tagetes erecta), onion (Allium cepa), garlic (Allium sativumL.), neem (Azadiracta indica), lime (Citrus aurantifolia), and turmeric (Curcuma longaL.) were tested for antibacterial activity against potato soft rot bacteria,E. carotovorasubsp.carotovora (Ecc)P-138, underin vitroand storage conditions. Previously,EccP-138 was identified as the most aggressive soft rot bacterium in Bangladeshi potatoes. Of the 11 different plant extracts, only extracts from dried jute leaves and cheerota significantly inhibited growth ofEccP-138in vitro. Finally, both plant extracts were tested to control the soft rot disease of potato tuber under storage conditions. In a 22-week storage condition, the treated potatoes were significantly more protected against the soft rot infection than those of untreated samples in terms of infection rate and weight loss. The jute leaf extracts showed more pronounced inhibitory effects onEcc-138 growth both inin vitroand storage experiments.

Download Full-text

Isolation, Characterization, and Identification of Biological Control Agent for Potato Soft Rot in Bangladesh

The Scientific World JOURNAL ◽

10.1100/2012/723293 ◽

2012 ◽

Vol 2012 ◽

pp. 1-6 ◽

Cited By ~ 8

Author(s):

M. M. Rahman ◽

M. E. Ali ◽

A. A. Khan ◽

A. M. Akanda ◽

Md. Kamal Uddin ◽

...

Keyword(s):

Biological Control ◽

Biological Control Agent ◽

Erwinia Carotovora ◽

Soft Rot ◽

Control Agent ◽

Antagonistic Activity ◽

Antagonistic Effect ◽

Antagonistic Bacteria ◽

And Storage

A total of 91 isolates of probable antagonistic bacteria of potato soft rot bacteriumErwinia carotovorasubsp.carotovora(Ecc) were extracted from rhizospheres and endophytes of various crop plants, different soil varieties, and atmospheres in the potato farming areas of Bangladesh. Antibacterial activity of the isolated probable antagonistic bacteria was testedin vitroagainst the previously identified most common and most virulent soft rot causing bacterial strain Ecc P-138. Only two isolates E-45 and E-65 significantly inhibited thein vitrogrowth of Ecc P-138. Physiological, biochemical, and carbon source utilization tests identified isolate E-65 as a member of the genusBacillusand the isolate E-45 asLactobacillussp. The stronger antagonistic activity against Ecc P-138 was found in E-65in vitroscreening and storage potatoes. E-65 reduced the soft rot infection to 22-week storage potatoes of different varieties by 32.5–62.5% in model experiment, demonstrating its strong potential to be used as an effective biological control agent for the major pectolytic bacteria Ecc. The highest (62.5%) antagonistic effect of E-65 was observed in the Granola and the lowest (32.7%) of that was found in the Cardinal varieties of the Bangladeshi potatoes. The findings suggest that isolate E-65 could be exploited as a biocontrol agent for potato tubers.

Download Full-text

Kemampuan Bakteri Endofit Akar dan Ubi Kentang untuk Menekan Penyakit Busuk Lunak (Erwinia carotovora pv. carotovora) pada Ubi

Agrikultura ◽

10.24198/agrikultura.v27i3.10880 ◽

2016 ◽

Vol 27 (3) ◽

Author(s):

Noor Istifadah ◽

Muhamad Salman Umar ◽

Sudarjat Sudarjat ◽

Luciana Djaya

Keyword(s):

Biological Control ◽

Potato Tuber ◽

Endophytic Bacteria ◽

Erwinia Carotovora ◽

Soft Rot ◽

Soft Rot Disease ◽

Post Harvest ◽

Rot Disease

ABSTRACTThe abilities of endophytic bacteria from potato roots and tubers to suppress soft rot disease (Erwinia carotovora pv. carotovora) in potato tuberSoft rot disease caused by Erwinia carotovora pv. carotovora is one of limiting factors in cultivation and post harvest of potato. The eco-friendly control measure that can be developed for controlling the diseases is biological control. Microbes that are potential as biological control agents include endophytic bacteria. This paper discussed the results of study examining the potential of endophytic bacteria isolated from roots and tubers of potato to inhibit the growth of E. carotovora pv. carotovora in vitro and suppress soft rot disease in potato tuber. The results showed that among 24 isolates examined, four isolates of endophytic bacteria (one isolate from potato tuber and three isolates from potato roots) inhibited the growth of E. carotovora pv. carotovora in vitro with inhibition zone 3.5-6.8 mm. In the in vivo test, the isolates inhibited the soft rot disease in potato tuber by 71.5-86.4%. The isolate that tended to show relatively better inhibition in vitro and in vivo was isolate from potato tuber which is CK U3 (Lysinibacillus sp.)Keywords: Biological control, Endophytic bacteria, Post-harvest, Potato, Soft rot diseaseABSTRAKPenyakit busuk lunak yang disebabkan bakteri Erwinia carotovora pv. carotovora, merupakan salah satu kendala dalam budidaya dan pascapanen kentang. Cara pengendalian ramah lingkungan yang dapat dikembangkan untuk menekan penyakit tersebut adalah pengendalian biologi. Kelompok mikroba yang berpotensi sebagi agens pengendali biologi adalah bakteri endofit. Artikel ini mendiskusikan potensi isolat bakteri endofit yang berasal dari ubi dan akar kentang untuk menghambat pertumbuhan bakteri E. carotovora pv. carotovora secara in vitro dan menekan perkembangan penyakit busuk lunak pada ubi kentang. Hasil percobaan menunjukkan bahwa diantara 24 isolat bakteri yang diuji, terdapat empat isolat bakteri endofit (satu isolat dari ubi kentang dan tiga isolat dari akar kentang) yang dapat menghambat pertumbuhan bakteri E. carotovora pv. carotovora secara in vitro dengan zona penghambatan sebesar 3,5-6,8 mm. Pada pengujian secara in vivo, isolat-isolat tersebut dapat menekan perkembangan penyakit busuk lunak pada ubi kentang sebesar 71,5-86,4%. Isolat yang cenderung menunjukkan penghambatan relatif lebih baik secara in vitro dan in vivo adalah isolat bakteri endofit asal ubi kentang yaitu isolat CK U3 (Lysinibacillus sp.).Kata Kunci: Pengendalian biologi, Bakteri endofit, Pascapanen, Kentang, Penyakit busuk basah

Download Full-text

Improving the Biocontrol Potential of Bacterial Antagonists with Salicylic Acid against Brown Rot Disease and Impact on Nectarine Fruits Quality

Agronomy ◽

10.3390/agronomy11020209 ◽

2021 ◽

Vol 11 (2) ◽

pp. 209

Author(s):

Nadia Lyousfi ◽

Rachid Lahlali ◽

Chaimaa Letrib ◽

Zineb Belabess ◽

Rachida Ouaabou ◽

...

Keyword(s):

Salicylic Acid ◽

Disease Severity ◽

Mycelial Growth ◽

Brown Rot ◽

Antagonistic Bacteria ◽

Biological Treatments ◽

Rot Disease ◽

The Impact

The main objective of this study was to evaluate the ability of both antagonistic bacteria Bacillus amyloliquefaciens (SF14) and Alcaligenes faecalis (ACBC1) used in combination with salicylic acid (SA) to effectively control brown rot disease caused by Monilinia fructigena. Four concentrations of salicylic acid (0.5%, 2%, 3.5%, and 5%) were tested under in vitro and in vivo conditions. Furthermore, the impact of biological treatments on nectarine fruit parameters’ quality, in particular, weight loss, titratable acidity, and soluble solids content, was evaluated. Regardless of the bacterium, the results indicated that all combined treatments displayed a strong inhibitory effect on the mycelial growth of M. fructigena and disease severity. Interestingly, all SA concentrations significantly improved the biocontrol activity of each antagonist. The mycelial growth inhibition rate ranged from 9.79% to 88.02% with the highest reduction rate recorded for bacterial antagonists in combination with SA at both concentrations of 0.5% and 3.5%. The in vivo results confirmed the in vitro results with a disease severity varying from 0.00% to 51.91%. A significant biocontrol improvement was obtained with both antagonistic bacteria when used in combination with SA at concentrations of 0.5% and 2%. The lowest disease severity observed with ACBC1 compared with SF14 is likely due to a rapid adaptation and increase of antagonistic bacteria population in wounded sites. The impact of all biological treatments revealed moderate significant changes in the fruit quality parameters with weight loss for several treatments. These results suggest that the improved disease control of both antagonistic bacteria was more likely directly linked to both the inhibitory effects of SA on pathogen growth and induced fruit resistance.

Download Full-text

The Ribosomal Protein RplY Is Required for Pectobacterium carotovorum Virulence and Is Induced by Zantedeschia elliotiana Extract

Phytopathology ◽

10.1094/phyto-04-17-0161-r ◽

2017 ◽

Vol 107 (11) ◽

pp. 1322-1330 ◽

Cited By ~ 6

Author(s):

Huan Jiang ◽

Mengyi Jiang ◽

Liuke Yang ◽

Peiyan Yao ◽

Lin Ma ◽

...

Keyword(s):

Plant Extract ◽

Plant Cell Wall ◽

Insertion Site ◽

Bacterial Pathogen ◽

Soft Rot ◽

Kanamycin Resistance ◽

Pectobacterium Carotovorum ◽

Promoter Trap ◽

Carotovorum Subsp ◽

Rot Disease

Pectobacterium carotovorum subsp. carotovorum strain PccS1, a bacterial pathogen causing soft rot disease of Zantedeschia elliotiana (colored calla), was investigated for virulence genes induced by the host plant. Using a promoter-trap transposon (mariner), we obtained 500 transposon mutants showing kanamycin resistance dependent on extract of Z. elliotiana. One of these mutants, PM86, exhibited attenuated virulence on both Z. elliotiana and Brassica rapa subsp. pekinensis. The growth of PM86 was also reduced in minimal medium (MM), and the reduction was restored by adding plant extract to the MM. The gene containing the insertion site was identified as rplY. The deletion mutant ΔrplY, exhibited reduced virulence, motility and plant cell wall-degrading enzyme production but not biofilm formation. Analysis of gene expression and reporter fusions revealed that the rplY gene in PccS1 is up-regulated at both the transcriptional and the translational levels in the presence of plant extract. Our results suggest that rplY is induced by Z. elliotiana extract and is crucial for virulence in P. carotovorum subsp. carotovorum.

Download Full-text

Evaluating the Antibacterial Activity and Mode of Action of Thymol-Loaded Chitosan Nanoparticles Against Plant Bacterial Pathogen Xanthomonas campestris pv. campestris

Frontiers in Microbiology ◽

10.3389/fmicb.2021.792737 ◽

2022 ◽

Vol 12 ◽

Author(s):

Sarangapani Sreelatha ◽

Nadimuthu Kumar ◽

Tan Si Yin ◽

Sarojam Rajani

Keyword(s):

Electron Microscopy ◽

Antibacterial Activity ◽

Xanthomonas Campestris ◽

Membrane Damage ◽

Chitosan Nanoparticles ◽

Bacterial Pathogen ◽

Electron Microscopy Analysis ◽

Rot Disease ◽

The Impact

The bacterium Xanthomonas campestris pv. campestris (Xcc) causes black rot disease in cruciferous crops, resulting in severe yield loss worldwide. The excessive use of chemical pesticides in agriculture to control diseases has raised significant concern about the impact on the environment and human health. Nanoparticles have recently gained significant attention in agriculture owing to their promising application in plant disease control, increasing soil fertility and nutrient availability. In the current study, we synthesized thymol-loaded chitosan nanoparticles (TCNPs) and assessed their antibacterial activity against Xcc. The synthesis of TCNPs was confirmed by using ultraviolet–visible spectroscopy. Fourier-transform infrared spectroscopy, transmission electron microscopy, and scanning electron microscopy analysis revealed the functional groups, size, and shape of TCNPs, with sizes ranging from 54 to 250 nm, respectively. The antibacterial activity of TCNPs against Xcc was investigated in vitro by liquid broth, cell viability, and live dead staining assay, and all of them demonstrated the antibacterial activity of TCNPs. Furthermore, TCNPs were found to directly inhibit the growth of Xcc by suppressing the growth of biofilm formation and the production of exopolysaccharides and xanthomonadin. The ultrastructure studies revealed membrane damage in TCNP-treated Xcc cells, causing a release of intracellular contents. Headspace/gas chromatography (GC)–mass spectrometry (MS) analysis showed changes in the volatile profile of Xcc cells treated with TCNPs. Increased amounts of carbonyl components (mainly ketones) and production of new volatile metabolites were observed in Xcc cells incubated with TCNPs. Overall, this study reveals TCNPs as a promising antibacterial candidate against Xcc.

Download Full-text

Biological Control Efficacy of Bacillus Licheniformis Hg03 Against Soft Rot Disease of Postharvest Peach

SSRN Electronic Journal ◽

10.2139/ssrn.4000312 ◽

2022 ◽

Author(s):

Yunpeng Wang ◽

Xiaoli Wang ◽

Jingfeng Zhu ◽

Huan Wei ◽

Zhipeng Ding ◽

...

Keyword(s):

Biological Control ◽

Bacillus Licheniformis ◽

Soft Rot ◽

Control Efficacy ◽

Soft Rot Disease ◽

Rot Disease

Download Full-text

Screening, Identification and Efficacy Evaluation of Antagonistic Bacteria for Biocontrol of Soft Rot Disease Caused by Dickeya zeae

Microorganisms ◽

10.3390/microorganisms8050697 ◽

2020 ◽

Vol 8 (5) ◽

pp. 697 ◽

Cited By ~ 1

Author(s):

Jieling Li ◽

Ming Hu ◽

Yang Xue ◽

Xia Chen ◽

Guangtao Lu ◽

...

Keyword(s):

Antimicrobial Activity ◽

Prevention And Control ◽

Pathogenic Bacteria ◽

Soft Rot ◽

Antagonistic Bacteria ◽

Bacterial Soft Rot ◽

Soft Rot Disease ◽

Wide Range ◽

Rot Disease ◽

And Control

Dickeya zeae is the causal agent of bacterial soft rot disease, with a wide range of hosts all over the world. At present, chemical agents, especially agricultural antibiotics, are commonly used in the prevention and control of bacterial soft rot, causing the emergence of resistant pathogens and therefore increasing the difficulty of disease prevention and control. This study aims to provide a safer and more effective biocontrol method for soft rot disease caused by D. zeae. The spot-on-lawn assay was used to screen antagonistic bacteria, and three strains including SC3, SC11 and 3-10 revealed strong antagonistic effects and were identified as Pseudomonas fluorescens, P. parafulva and Bacillus velezensis, respectively, using multi-locus sequence analysis (MLSA) based on the sequences of 16S rRNA and other housekeeping genes. In vitro antimicrobial activity showed that two Pseudomonas strains SC3 and SC11 were only antagonistic to some pathogenic bacteria, while strain 3-10 had broad-spectrum antimicrobial activity on both pathogenic bacteria and fungi. Evaluation of control efficacy in greenhouse trials showed that they all restrained the occurrence and development of soft rot disease caused by D. zeae MS2 or EC1. Among them, strain SC3 had the most impressive biocontrol efficacy on alleviating the soft rot symptoms on both monocotyledonous and dicotyledonous hosts, and strain 3-10 additionally reduced the occurrence of banana wilt disease caused by Fusarium oxysporum f. sp. cubensis. This is the first report of P. fluorescens, P. parafulva and B. velezensis as potential bio-reagents on controlling soft rot disease caused by D. zeae.

Download Full-text

Effect of some chemicals on incidence of poato soft rot disease in Bangladesh

Bangladesh Journal of Scientific and Industrial Research ◽

10.3329/bjsir.v52i2.32936 ◽

2017 ◽

Vol 52 (2) ◽

pp. 135-140 ◽

Cited By ~ 1

Author(s):

MM Rahman ◽

AA Khan ◽

IH Mian ◽

AM Akanda ◽

MZ Alam

Keyword(s):

Acetic Acid ◽

Boric Acid ◽

Soft Rot ◽

Pectobacterium Carotovorum ◽

Potato Tubers ◽

Soft Rot Disease ◽

Bleaching Powder ◽

Carotovorum Subsp ◽

Rot Disease

Bactericidal effect was investigated by chemicals against potato soft rot bacteria in vitro and in storage. The chemicals were acetic acid, boric acid, bleaching powder, lactic acid, calcium hydroxide, calcium chloride, potassium chloride and sodium hypo-chloride. Among eight chemicals only three chemicals viz. acetic acid, boric acid and bleaching powder showed bactericidal activity against potato soft rot bacteria Pectobacterium carotovorum subsp. carotovorum (E. carotovora subsp. carotovora) P-138 in vitro. Based on the results of in vitro experiment three chemicals, acetic acid, boric acid and bleaching powder were used to control soft rot disease of potato in storage. Fresh potato tubers were dipped in 0.2% solution/suspensions of acetic acid, boric acid and bleaching powder for 30 min. Then soft rot bacteria Pectobacterium carotovorum subsp. carotovorum P-138 was inoculated on potato. Finally potatoes were stored for 22 weeks in net bags in sterilized condition. All the three chemicals significantly decreased the infection rate, loss in weight and increased percentage of disease reduction (PDR) of potato. Boric acid was the most effective in controlling the soft rot disease of potato in storage followed by acetic acid and bleaching powder. So these chemicals may be used for seed purpose storage of potato tubers for year round storage at farmers level.Bangladesh J. Sci. Ind. Res. 52(2), 135-140, 2017

Download Full-text

The Iturin-like Lipopeptides Are Essential Components in the Biological Control Arsenal of Bacillus subtilis Against Bacterial Diseases of Cucurbits

Molecular Plant-Microbe Interactions ◽

10.1094/mpmi-06-11-0162 ◽

2011 ◽

Vol 24 (12) ◽

pp. 1540-1552 ◽

Cited By ~ 74

Author(s):

Houda Zeriouh ◽

Diego Romero ◽

Laura García-Gutiérrez ◽

Francisco M. Cazorla ◽

Antonio de Vicente ◽

...

Keyword(s):

Biological Control ◽

Antibacterial Activity ◽

Bacillus Subtilis ◽

Xanthomonas Campestris ◽

Soft Rot ◽

Bacterial Diseases ◽

Chromatography Analysis ◽

Essential Components ◽

Bacillus Spp

The antibacterial potential of four strains of Bacillus subtilis, UMAF6614, UMAF6619, UMAF6639, and UMAF8561, previously selected on the basis of their antifungal activity and efficacy against cucurbit powdery mildew, was examined. Among these strains, UMAF6614 and UMAF6639 showed the highest antibacterial activity in vitro, especially against Xanthomonas campestris pv. cucurbitae and Pectobacterium carotovorum subsp. carotovorum. These strains produced the three families of lipopeptide antibiotics known in Bacillus spp.: surfactins, iturins, and fengycins. Using thin-layer chromatography analysis and direct bioautography, the antibacterial activity could be associated with iturin lipopeptides. This result was confirmed by mutagenesis analysis using lipopeptide-defective mutants. The antibacterial activity was practically abolished in iturin-deficient mutants, whereas the fengycin mutants retained certain inhibitory capabilities. Analyses by fluorescence and transmission electron microscopy revealed the cytotoxic effect of these compounds at the bacterial plasma membrane level. Finally, biological control assays on detached melon leaves demonstrated the ability of UMAF6614 and UMAF6639 to suppress bacterial leaf spot and soft rot; accordingly, the biocontrol activity was practically abolished in mutants deficient in iturin biosynthesis. Taken together, our results highlight the potential of these B. subtilis strains as biocontrol agents against fungal and bacterial diseases of cucurbits and the versatility of iturins as antifungal and antibacterial compounds.

Download Full-text