scholarly journals VISCERAL LEISHMANIASIS IS ENDEMIC IN GOLDEN JACKALS OF BANGLADESH AGRICULTURAL UNIVERSITY CAMPUS, A THREAT FOR EXPANDING FUTURE ZOONOTIC VISCERAL LEISHMANIASIS

2013 ◽  
Vol 10 (1-2) ◽  
pp. 101-109 ◽  
Author(s):  
M. A. H. N. A. Khan ◽  
S. S. Khanm ◽  
J. Bashu ◽  
U. K. Rima ◽  
M. Pervin ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Visceral Leishmaniasis ◽  
Leishmania Donovani ◽  
Venous Blood ◽  
Fibrous Tissue ◽  
Western China ◽  
Histopathological Study ◽  
Chronic Glomerulonephritis ◽  
University Campus ◽  
Species Specific

Visceral leishmaniasis (VL), now a day, is an endemic protozoal disease of human in Bangladesh. The disease is transmissible to canids and endemic in canids in many parts of the world including western China and India. In Bangladesh, the occurrence of VL in canids is unknown. Golden jackals, a common wild canid of Bangladesh Agricultural University (BAU) Campus, living with street dogs, cats and often get closure to human shelter. It is not surprising to find VL in golden Jackals of BAU campus, Mymensingh and this study was, therefore, aimed to identify the occurrence of leishmanial infection in their visceral organs. Venous blood, liver, kidney, spleen and bone marrow from the jackals (N=5) were collected, impression smears from these organs were made onto clean slides and stained with Giemsa’s stain. Leishmanial pro - and amastigote stages of the parasite were detected in the impression smears made from liver, spleen, kidney and bone marrow in all jackals. Leishmanial amastigote stage of the parasite was seen in the macrophages of blood, spleen and liver. Part of liver, kidney, spleen and bone marrow from the jackal were fixed in 10% buffered neutral formalin and stained with hematoxylin and eosin (H&E). Histopathological study revealed scatteredly distributed microgranuloma in the hepatic parenchyma, consisting of closely packed collection of macrophages and lymphocytes with fibrous tissue encapsulation. In kidney, chronic glomerulonephritis was seen; the inflamed areas were infiltrated with lymphocytes and macrophages. Specific pathology in spleen and bone marrow was not observed. Genomic DNA was extracted from the liver, kidney, spleen and bone marrow of jackals. Polymerase chain reaction (PCR) with species specific primers were used to identify the species of leishmanial protozoa involved. Results of PCR showed 145bp amplicon, specific for Leishmania donovani infection in all five jackals. This is the first report in Bangladesh describing the occurrence of VL in golden jackals. This result reinforce the assumption that golden jackals of BAU campus harbour leishmanial protozoa, could serve as a symptomless carrier and transmit the infection to other canids and human, require further investigation.DOI: http://dx.doi.org/10.3329/bjvm.v10i1-2.15655

scholarly journals Comparison of KAtex, Bone Marrow Aspiration and DAT for the Diagnosis of Visceral Leishmaniasis

2019 ◽  
Vol 6 (1) ◽  
pp. 12-15
Author(s):  
Ishrat Sharmin ◽  
AKM Quamruzzaman ◽  
Rezina Parveen ◽  
M Abdulah Yusuf ◽  
Rashida Akter Khanam
Keyword(s):  
Bone Marrow ◽  
Visceral Leishmaniasis ◽  
Leishmania Donovani ◽  
Bone Marrow Aspiration ◽  
Cross Sectional Study ◽  
Cross Sectional ◽  
Medical College ◽  
Kala Azar ◽  
History Of ◽  
One Year

Background: Newly developed KAtex test can be used as a non invasive tool for diagnosis of Kala-azar. Objectives: The aim of the present study was to compare KAtex, Bone marrow aspiration and DAT to diagnose VL. Methodology: This cross-sectional study was carried out in the Department of Microbiology at Dhaka Medical College, Dhaka, Bangladesh in collaboration with the Department of Parasitology, Institute of Epidemiology, Disease Control and Research (IEDCR), Dhaka, Bangladesh for a period of one year. Clinically suspected Kala-azar (VL) cases of different age and sex attending IEDCR, Dhaka from different Kala-azar endemic areas of Bangladesh were selected for this study. Patients having fever for more than 2 weeks, with or without splenomegaly, having history of loss of body weight following onset of fever were clinically suspected as Kala-azar cases. Microscopy and culture was performed in bone marrow (BM). KAtex was performed with urine sample. Agglutination of sensitized latex indicated presence of Leishmania donovani antigen in urine and thereby visceral leishmaniasis. No agglutination indicates absence of antigen in urine. DAT was done with serums of all cases. Result: Among 130 clinically suspected VL cases, 70 (53.85%) cases were BM positive and 60(46.15%) cases were BM negative. All the 70 BM positive cases were positive by KAtex and DAT. Among 60 BM negative cases, 15 were positive by KAtex and 23 were positive by DAT. The sensitivity of KAtex was 100.0% and specificity was 75.0%. The sensitivity of DAT was 100.0% and specificity is 61.6%. Conclusion: In conclusion, KAtex test is a good diagnostic tool for the detection of VL in comparison with DAT. Bangladesh Journal of Infectious Diseases, June 2019;6(1):12-15

scholarly journals An acute presentation of haemophagocytic lymphohistiocytosis due to visceral leishmaniasis in a British adult returning traveller

2019 ◽  
Vol 18 (3) ◽  
pp. 184-188
Author(s):  
Anastasia A Theodosiou ◽  
◽  
Hwai Jing Hiew ◽  
Christina Petridou ◽  
◽  
...  
Keyword(s):  
Bone Marrow ◽  
Visceral Leishmaniasis ◽  
Leishmania Donovani ◽  
Bone Marrow Aspirate ◽  
Caucasian Woman ◽  
Haemophagocytic Lymphohistiocytosis ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
The Uk ◽  
Very High

A 62-year old British Caucasian woman normally resident in Spain presented with fever and pancytopaenia after returning to the UK. Her symptoms persisted despite broad-spectrum antibiotics, and she gradually became confused, hypotensive and progressively more pancytopaenic. Imaging demonstrated hepatosplenomegaly, and a bone marrow aspirate confirmed a diagnosis of haemophagocytic lymphohistiocytosis (HLH). Bone marrow polymerase chain reaction (PCR) and blood serology were both positive for Leishmania donovani, consistent with visceral leishmaniasis (VL). Following treatment with dexamethasone and amphotericin, she improved clinically and biochemically, and was able to return to Spain. Fever in the returning traveller is a common acute medical presentation. Although HLH and VL are rare diagnoses, both carry a very high mortality rate if undiagnosed and untreated.

scholarly journals Comparative Study of Microscopy and Polymerase Chain Reaction for the Diagnosis of Suspected Visceral Leishmaniasis Patients in Nepal

2014 ◽  
Vol 11 (1) ◽  
pp. 14-17 ◽  
Author(s):  
K Pandey ◽  
AK Mallik ◽  
S Pyakurel ◽  
SB Pun ◽  
BD Pandey
Keyword(s):  
Bone Marrow ◽  
Polymerase Chain Reaction ◽  
Visceral Leishmaniasis ◽  
Leishmania Donovani ◽  
Polymerase Chain Reaction Amplification ◽  
Public Health Problem ◽  
Major Public Health Problem ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Endemic Areas

Background Visceral leishmaniasis is potentially fatal protozoan diseases caused by Leishmania donovani. Nepal is an endemic region in which visceral leishmaniasis causes a major public health problem in the lowland areas that border the endemic areas of Bihar state in India. Accurate diagnosis to inform treatment is a first step in achieving the goal of visceral leishmaniasis elimination from South East Asian regions by 2020. Objective The objective of the present study was to compare between the Microcopy and polymerase chain reaction for diagnosis of visceral leishmaniasis. Methods In the present study, 236 bone marrow aspirations were collected from suspected visceral leishmaniasis patients in Janakpur Zonal Hospital, Dhanusa district, Terai region of Nepal in between 2003-2007. We evaluated bone marrow samples by microscopic examination with subsequent testing of the same sample by polymerase chain reaction and sequence analysis. Results Giemsa’s solution stained bone marrow slides stored for over five years were used for polymerase chain reaction amplification. The result showed that 71% were polymerase chain reaction positive and 56% were microscopic positive. Out of 104 microscopic negative bone marrow samples, 15% of samples were positive by polymerase chain reaction. Conclusion Polymerase chain reaction could make a very good option for diagnosis by using less or non-invasive material from visceral leishmaniasis patients in endemic areas of Nepal. DOI: http://dx.doi.org/10.3126/kumj.v11i1.11016 Kathmandu University Medical Journal Vol.11(1) 2013: 14-17

scholarly journals Epistaxis in Visceral Leishmaniasis: A hospital based study in Eastern Nepal

2012 ◽  
Vol 1 (1) ◽  
pp. 38-40
Author(s):  
B Sigdel ◽  
S Bhandary ◽  
P Karki ◽  
A Ghimire
Keyword(s):  
Bone Marrow ◽  
Visceral Leishmaniasis ◽  
Clinical Manifestation ◽  
Endemic Area ◽  
Leishmania Donovani ◽  
Age Group ◽  
Peripheral Smear ◽  
Cross Sectional ◽  
Severe Epistaxis ◽  
Endemic Areas

INTRODUCTION: Epistaxis is a common clinical manifestation in endemic areas of Visceral Leishmaniasis (VL). This study aims to see the prevalence of epistaxis in VL in Eastern Nepal. MATERIALS AND METHODS: This study was a prospective cross sectional hospital based study. Diagnosis was made by Rk-39 from peripheral smear & Leishmania donovani (LD) bodies from bone marrow. Before starting anti–kalaazar treatment, nasal examination findings were noted. RESULTS: Ninenteen out of 80 diagnosed case of VL patients had epistaxis (23.8%) with age group between 7-66 years. One patient had developed severe epistaxis and hemotoma and died. CONCLUSION: Epistaxis is a common ent finding in endemic area of VL, however this needs to be confirm with larger cohorts. DOI: http://dx.doi.org/10.3126/ijim.v1i1.6942Int J Infect Microbiol 2012;1(1):38-40

scholarly journals Hybrid Cell Vaccination Resolves Leishmania donovani Infection by Eliciting a Strong CD8+ Cytotoxic T-Lymphocyte Response with Concomitant Suppression of Interleukin-10 (IL-10) but Not IL-4 or IL-13

2007 ◽  
Vol 75 (12) ◽  
pp. 5956-5966 ◽  
Author(s):  
Rajatava Basu ◽  
Suniti Bhaumik ◽  
Arun Kumar Haldar ◽  
Kshudiram Naskar ◽  
Tripti De ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Immune Response ◽  
T Cell ◽  
Visceral Leishmaniasis ◽  
T Lymphocyte ◽  
Leishmania Donovani ◽  
Cytotoxic T Lymphocyte ◽  
Hybrid Cell ◽  
Cell Depletion ◽  
T Cell Depletion

ABSTRACT There is an acute dearth of therapeutic interventions against visceral leishmaniasis that is required to restore an established defective cell-mediated immune response. Hence, formulation of effective immunotherapy requires the use of dominant antigen(s) targeted to elicit a specific antiparasitic cellular immune response. We implemented hybrid cell vaccination therapy in Leishmania donovani-infected BALB/c mice by electrofusing dominant Leishmania antigen kinetoplastid membrane protein 11 (KMP-11)-transfected bone marrow-derived macrophages from BALB/c mice with allogeneic bone marrow-derived dendritic cells from C57BL/6 mice. Hybrid cell vaccine (HCV) cleared the splenic and hepatic parasite burden, eliciting KMP-11-specific major histocompatibility complex class I-restricted CD8+ cytotoxic T-lymphocyte (CTL) responses. Moreover, splenic lymphocytes of HCV-treated mice not only showed the enhancement of gamma interferon but also marked an elevated expression of the Th2 cytokines interleukin-4 (IL-4) and IL-13 at both transcriptional and translational levels. On the other hand, IL-10 production from splenic T cells was markedly suppressed as a result of HCV therapy. CD8+ T-cell depletion completely abrogated HCV-mediated immunity and the anti-KMP-11 CTL response. Interestingly, CD8+ T-cell depletion completely abrogated HCV-induced immunity, resulting in a marked increase of IL-10 but not of IL-4 and IL-13. The present study reports the first implementation of HCV immunotherapy in an infectious disease model, establishing strong antigen-specific CTL generation as a correlate of HCV-mediated antileishmanial immunity that is reversed by in vivo CD8+ T-cell depletion of HCV-treated mice. Our findings might be extended to drug-nonresponsive visceral leishmaniasis patients, as well as against multiple infectious diseases with pathogen-specific immunodominant antigens.

scholarly journals Canine visceral leishmaniasis in Rondônia, Brazil: a report of an autochthonous case

2021 ◽  
Vol 15 (1) ◽  
pp. 15-18
Author(s):  
Henrique Momo Ziemniczak ◽  
Guilherme Henrique Lemes da Silva ◽  
Maerle Oliveira Maia ◽  
Elvino Ferreira ◽  
Klaus Casaro Saturnino ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Lymph Node ◽  
Visceral Leishmaniasis ◽  
Leishmania Donovani ◽  
Needle Aspiration ◽  
Popliteal Lymph Node ◽  
Canine Visceral Leishmaniasis ◽  
Tissue Samples ◽  
Control Center ◽  
Autochthonous Case

Visceral Leishmaniasis (VL), also known as kala-azar, is a disseminated protozoan infection caused by the Leishmania donovani complex. Traditionally, the definitive diagnosis is made by detecting amastigotes in biological tissue samples. In August 2015, an apparently healthy, young adult, female, mongrel dog from the Zoonosis Control Center of the Rolim de Moura,Rondônia, was subjected to the canine visceral leishmaniasis diagnosis. The diagnosis was conducted using bone marrow aspirate smears and popliteal lymph node smears by Fine-needle aspiration biopsy (FNAB); stained slides with Diff-Quick screened for Leishmania amastigotes by means of direct optical microscopic examination (100×). Lymph node and bone marrow aspirates were used to investigate the presence of Leishmania infantum chagasi DNA by real-time PCR. The popliteal lymph node positive result was observed in typical amastigotes, presenting nucleus and kinetoplasts associated with lymphoblastic proliferation, reactive macrophages, plasmocytes (commonly Mott cells), eosinophils, and lymphoglandular hyperplasia. A positive bone marrow sample resulted from the observation of amastigotes in the monocyte cytoplasm or free on the smear background. The presence of the L. infantum kDNA was detected in lymph node and bone marrow FNAB samples. This research note describes the autochthonous case of CVL recorded in the state of Rondônia, Brazil, a non-endemic area for VL. According to the data, future studies must include a larger number of animals to elucidate the parasite’s epidemiological resource in Rondônia.

Comparison of the efficacies of various formulations of amphotericin B against murine visceral leishmaniasis.

1997 ◽  
Vol 41 (10) ◽  
pp. 2089-2092 ◽  
Author(s):  
A B Mullen ◽  
K C Carter ◽  
A J Baillie
Keyword(s):  
Bone Marrow ◽  
Body Weight ◽  
Visceral Leishmaniasis ◽  
Amphotericin B ◽  
Murine Model ◽  
Leishmania Donovani ◽  
Significant Suppression ◽  
Dosing Regimen ◽  
Multiple Dosing ◽  
Lipid Complex

The antileishmanial efficacies of four proprietary amphotericin B (AmB) formulations (Fungizone, AmBisome, Abelcet, and Amphocil) and an experimental nonionic surfactant vesicle (NIV) formulation were compared in a murine model of acute visceral leishmaniasis. By a multiple-dosing regimen, groups of Leishmania donovani-infected BALB/c mice were treated (2.5 mg of AmB per kg of body weight) on days 7 to 11 postinfection with one of the AmB formulations, and parasite burdens were determined on day 18 postinfection. All of the formulations caused significant suppression parasite burdens in spleens (P < 0.01 to 0.0005) and livers (P < 0.0005) compared with those in the spleens and livers of the controls. In addition, a significant suppression of parasite burdens in bone marrow (P < 0.0005) compared to the burdens in the bone marrow of the controls was obtained for all the formulations except Abelcet, which was inactive at this site. On the basis of their overall efficacies (activity against liver, spleen, and bone marrow parasites), the formulations could be ranked as follows: Amphocil = AmBisome > AmB-NIV > Abelcet > Fungizone. On the basis of spectrophotometric measurements, AmB was shown to exist in a predominantly aggregated state in all of the formulations. Although incubation in 50% serum altered the degree of aggregation, the AmB remained predominantly aggregated, indicating that the AMB-lipid complex in all of the formulations was physically stable. The results of the study showed that antiparasitic efficacy is associated positively with the degree of AmB aggregation in the presence of serum.

scholarly journals Diagnosing human asymptomatic visceral leishmaniasis in an urban area of the State of Minas Gerais, using serological and molecular biology techniques

2006 ◽  
Vol 39 (5) ◽  
pp. 421-427 ◽  
Author(s):  
Elizabeth Castro Moreno ◽  
Maria Norma Melo ◽  
José Roberto Lambertucci ◽  
José Carlos Serufo ◽  
Antero S.R. Andrade ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Urban Area ◽  
Leishmania Donovani ◽  
Venous Blood ◽  
Clinical Signs ◽  
Epidemiological Studies ◽  
Southeastern Brazil ◽  
Enzyme Linked Immunosorbent Assay ◽  
Immunochromatographic Strip ◽  
Cross Sectional

A population-based cross-sectional study was set up in Sabará country, Southeastern Brazil, to identify asymptomatic human visceral leishmaniasis in an urban area of low disease prevalence. Blood was collected on filter paper (n=1,604 inhabitants) and examined by indirect immunofluorescent test, enzyme-linked immunosorbent assay and immunochromatographic strip test. The prevalence rates of infection ranged from 2.4 to 5.6% depending on the test used. One year later, venous blood was collected in a subset of 226 participants (102 seropositive and 124 seronegative). The tests performed were IFAT, ELISA, rk39-ELISA, polymerase chain reaction and hybridization with Leishmania donovani complex probe. No clinical signs or symptoms of leishmaniasis were observed. Using hybridization as a reference test, the sensitivity and specificity of serology were respectively: 24.8 and 71% (ELISA); 26.3 and 76.3% (rk-39); 30.1 and 63.4% (IFAT). Due to disagreements, different criteria were tested to define the infection and hybridization should be considered in epidemiological studies.

scholarly journals Bone marrow smear VS Blood smear in diagnosis of Kala-azar

2016 ◽  
Vol 3 (2) ◽  
pp. 38-42
Author(s):  
Nagendra Prasad Yadav ◽  
K D Das ◽  
R K Yadav
Keyword(s):  
Bone Marrow ◽  
Blood Sample ◽  
Blood Smear ◽  
Leishmania Donovani ◽  
Venous Blood ◽  
Laboratory Diagnosis ◽  
High Titre ◽  
Cross Sectional ◽  
Kala Azar ◽  
Bone Marrow Specimen

Background  and Objectives:  Bone marrow specimen is considered as superior to the blood in the laboratory diagnosis of Kala-azar. The  main objective of this study is to compare these two methods of diagnosis and determine the usefulness of the diagnostic techniques.Material and Methods:  This  prospective  cross sectional  study  was  conducted at Janakpur Zonal Hospital, Janakpur which was  aimed  to  determine  the usefulness of the bone marrow specimen and blood specimen in the  laboratory diagnosis of Kala-azar. Bone marrow aspirate and venous blood  was  collected  aseptically  from  the  cases  were  processed  simultaneously.  The  results  of these two cultures were compared. Results:  Total 60 cases  of Kala-azar were included in the study of which 32 were male  and 28 were  female.   Amastigote  form  of  Leishmania  donovani  were  detected  in  56  (93.33%)  samples with  high  titre  of  parasitemiae  and  119  (18%)  in  the  blood  sample  with  low  parasitemiae. Sensitivity and Specificity of the test was calculated of the bone marrow sample test have more sensitivity (98%) and specificity (100%) over the sensitivity (90%) and specificity (96%) of blood smear test.Conclusion:  Bone marrow specimens were  found to be more useful than the blood sample in the  laboratory diagnosis of Kala-azar.Janaki Medical College Journal of Medical Sciences (2015) Vol. 3 (2): 38-42

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