scholarly journals Characterization and Antibiogram of Escherichia coli Associated with Mortality in Broilers and Ducklings in Bangladesh

1970 ◽  
Vol 2 (1) ◽  
pp. 9-14 ◽  
Author(s):  
MT Islam ◽  
MA Islam ◽  
MA Samad ◽  
SML Kabir
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Sensitivity ◽  
High Rate ◽  
E Coli ◽  
Sensitivity Pattern ◽  
Highly Sensitive ◽  
Biochemical Studies ◽  
Increasing Trend ◽  
Escherichia Coli Infection ◽  
Hemorrhagic Enteritis

Mortality in broilers (6.56%) and growing ducks (11.0%) caused by Escherichia coli was recorded in the experimental flocks study during the period from May to August 2003. E. coli organisms isolated from broiler birds affected with characteristic lesions of omphalitis and yolk sac infection, fibrinous pericarditis and peri-hepatitis , hemorrhagic enteritis, and accumulation of excessive pericardial and peritoneal fluid, whereas from ducks with lesions of hemorrhagic enteritis and extensive epicardial hemorrhages. Each of the 21 isolates collected from broilers and 11 isolates from ducks was characterized by cultural and biochemical studies, of which 8 isolates from broilers and 5 isolates from ducks were tested for antibiotic sensitivity with 9 different antibiotics. The antibiotic sensitivity pattern showed that the isolates were highly sensitive to ciprofloxacin but an increasing trend of resistance was recorded in broilers (7 / 9) than duck (4 / 9) isolates. It may be concluded from the results of this study that the high rate of E. coli infection in broilers and ducks along with the high resistance of isolates to antibiotics constitute a threat to the poultry industry in Bangladesh. Key words: Escherichia coli infection; mortality; broilers; ducks; characterization; antibiogram doi: 10.3329/bjvm.v2i1.1927 Bangl. J. Vet. Med. (2004). 2 (1) : 09-14

scholarly journals Haemato-biochemical Changes and Antibiotic Sensitivity to Eschericia coli Associated with Concurrent Enteric and Septicaemic Infection in Calves

1970 ◽  
Vol 1 (1) ◽  
pp. 39-43 ◽  
Author(s):  
MA Samad ◽  
MA Islam ◽  
KA Hossain ◽  
MT Islam ◽  
S Saha
Keyword(s):  
Escherichia Coli ◽  
Serum Magnesium ◽  
Antibiotic Sensitivity ◽  
Blood Samples ◽  
E Coli ◽  
Drug Of Choice ◽  
Sensitivity Pattern ◽  
Highly Sensitive ◽  
Escherichia Coli Infection ◽  
First Time

Concurrent enteric and septicaemic Escherichia coli infection was recorded in six local calves aged between 8 to 12 months old for the first time in Bangladesh during the period from May to June 2002. Bacteriologic examination of both the faecal and blood samples of six febrile calves showed positive for E. coli of all the six faecal (100%) and only two blood (33.33%) samples. Clinical examination revealed significantly (p < 0.01) high rectal temperature at peak febrile stage which varied from 103.5 to 106°F with a mean of 104.7 ± 0.85°F. The fever associated signs and clinical anaemia were recorded in all these calves. Haematological examination showed significantly (p < 0.01) decreased haemoglobin (5.58 ± 0.45 g%), packed cell volume (19.83 ± 1.95 %), total erythrocytic count (3.52 ± 0.58  106/ mm3) and total leukocytic count (6.68 ± 0.44  103 / mm3) which indicates anaemia and leukopenia at the peak febrile stage of the disease. Biochemical studies showed significantly (p < 0.05) increased that serum magnesium and iron values were at fever stage, whereas serum phosphorus significantly (p < 0.01) decreased at the 4th day (convalescent stage) but no significant (p > 0.05) change was observed with the serum calcium, sodium, potassium and chloride values between prefever and fever stage. Antibiotic sensitivity pattern of E. coli isolated from faecal and blood samples revealed that these isolates were found to be resistant to penicillin, ampicillin and amoxycillin, moderately sensitive to gentamicin and streptomycin but highly sensitive to tetracycline. This indicates that tetracycline could be the drug of choice for both the septicaemic and enteric E. coli infection in calves. Key words: Haematobiochemical; Escherichia coli; enteric; septicemic; infection; calves DOI = 10.3329/bjvm.v1i1.1916 Bangl. J. Vet. Med.  (2003). 1(1) : 39 - 43

scholarly journals ISOLATION AND CHARACTERIZATION OF ESCHERICHIA COLI FROM BUFFALO CALVES IN SOME SELECTED AREAS OF BANGLADESH

1970 ◽  
Vol 8 (1) ◽  
pp. 23-26 ◽  
Author(s):  
SK Paul ◽  
MSR Khan ◽  
MA Rashid ◽  
J Hassan ◽  
SMS Mahmud
Keyword(s):  
Escherichia Coli ◽  
Nalidixic Acid ◽  
Rectal Swab ◽  
Antibiotic Sensitivity ◽  
Buffalo Calves ◽  
E Coli ◽  
Isolation And Characterization ◽  
Sensitivity Pattern ◽  
Highly Sensitive

The research works was conducted with a view to isolate and identify the Escherichia coli (E. coli) organism from diarrhoeic cases of buffalo reared in selected areas of Bangladesh as well the prevalence and antibiotic sensitivity pattern of the isolated E. coli in the Department of Microbiology and Hygiene, Bangladesh Agricultural University (BAU), Mymensingh-2202 during the period from April 2008 to May 2009. A total of 50 rectal swab samples were collected from 4 different places namely Haluaghat and Boira of Mymensingh, Madupur of Tangail and Kazipur of Sirajgonj districts. The samples were aseptically carried to the laboratory of the Department of Microbiology and Hygiene and subjected to different cultural, morphological and biochemical examinations. Upon cultural, morphological and biochemical examinations 23 (45%) samples were found to be positive for E. coli. The highest prevalence was found in Haluaghat, Mymensingh (53.33%) and the lowest (40.00%) in Boira, Mymensingh and Kazipur, Sirajganj. Antibiogram study revealed that the isolated E. coli was highly sensitive to Enrofloxacin and Ciprofloxacin, moderately sensitive to Cefalexin and Amoxicillin, and resistant to Nalidixic acid and Erythromycin. DOI = 10.3329/bjvm.v8i1.7398 Bangl. J. Vet. Med. (2010). 8(1): 23-26

scholarly journals Isolation, identification, toxin profile and antibiogram of Escherichia coli isolated from broilers and layers in Mymensingh district of Bangladesh

1970 ◽  
Vol 6 (1) ◽  
pp. 1-5 ◽  
Author(s):  
MT Hossain ◽  
MP Siddique ◽  
FMA Hossain ◽  
MA Zinnah ◽  
MM Hossain ◽  
...  
Keyword(s):  
Antibiotic Sensitivity ◽  
Inoculation Test ◽  
Infant Mouse ◽  
E Coli ◽  
Heat Stable ◽  
Sensitivity Pattern ◽  
Highly Sensitive ◽  
Poultry Farms ◽  
Increasing Trend ◽  
Apparently Healthy

The study was conducted to isolate and identify E. coli from apparently healthy broilers and layers from different poultry farms adjacent to the Bangladesh Agricultural University, Mymensingh, Bangladesh, during the period of January to May 2006 and characterize their ability to produce enterotoxin and also the antibiogram of the isolates. A total of 110 fecal samples were collected from broiler (n=55) and layer (n=55) chickens. E. coli were isolated and identified by cultural, biochemical, motility test and the heat-stable toxins were determined by Infant Mouse Assay (IMA). In case of broilers, 35 (63.6%) samples were found positive while 31 (56.4%) from layers. The overall prevalence of E. coli was 60%. Among the isolates of E. coli, 22.86% isolates from broiler and 38.71% isolates from layer were found positive for their ability to produce enterotoxin based on mice inoculation test. The antibiotic sensitivity pattern showed that the isolates were highly sensitive to chloramphenicol, ciprofloxacin, kenamycin and cephalexin and an increasing trend of resistance was recorded in both broiler and layer isolates. It may be concluded from the results of this study that the high resistance of E. coli to antibiotics constitutes a threat to poultry industry in Bangladesh.  Key words: E. coli, toxin, antibiogram DOI = 10.3329/bjvm.v6i1.1330 Bangl. J. Vet. Med. (2008). 6 (1): 01-05

scholarly journals Isolation and Detection of Antibiotic Sensitivity Pattern of Escherichia coli from Ducks in Bangladesh and Nepal

2013 ◽  
Vol 1 (1) ◽  
pp. 6-8
Author(s):  
Avishek Singh ◽  
Md Shahidur Rahman Khan ◽  
Sukumar Saha ◽  
Jayedul Hassan ◽  
Urmi Roy
Keyword(s):  
Escherichia Coli ◽  
Nalidixic Acid ◽  
Antibiotic Sensitivity ◽  
E Coli ◽  
Sensitivity Pattern ◽  
Highly Sensitive ◽  
Resistance Patterns ◽  
Typical Lesion ◽  
Different Origins ◽  
Biochemical Examination

A total of 120 rectal swab samples from ducks (sixty samples from Nepalgunj area of Nepal and 60 from Boyera area under Mymensingh district of Bangladesh) were collected for the isolation of Escherichia coli and their antibiogram study. After cultural and biochemical examination, a total of 40 samples from Nepalgunj and 45 samples from Boyera were found positive for E. coli. Pathogenicity study of 10 positive isolates from Nepalgunj and 12 positive isolates from Boyera were done to detect the presence of enterotoxin. All inoculated mice died showing typical lesion of extensive hemorrhage and massive edema. The isolates from two different origins showed major difference in their antibiogram study. The isolates of Nepal were highly sensitive to ciprofloxacin, co-trimoxazole, chloramphenicol and amoxicillin; moderately sensitive to nalidixic acid and; less sensitive to kanamycin and resistant to cephalexin. However, the isolates of Bangladesh were highly sensitive to ciprofloxacin, chloramphenicol and amoxicillin; moderately sensitive to nalidixic acid, cephalexin, and co-trimoxazole; less sensitive to kanamycin. This variation of antibiotic sensitivity and resistance patterns among the E. coli isolates of Nepal and Bangladesh might be due to strain variations and indiscriminate use of antibiotics in these two different countries.DOI: http://dx.doi.org/10.3329/mh.v1i1.13704 Microbes and Health Vol.1(1) June 2012 pp.6-8

scholarly journals Isolation Of Extended-Spectrum B-Lactamase (ESBL) Producing Escherichia coli and Klebsiella pneumiae From Dr. Zainoel Abidin General Hospital, Aceh

2019 ◽  
Vol 4 (1) ◽  
pp. 16-22 ◽  
Author(s):  
Zinatul Hayati ◽  
Syamsul Rizal ◽  
Ridhia Putri
Keyword(s):  
Escherichia Coli ◽  
General Hospital ◽  
Clinical Specimen ◽  
Antibiotic Sensitivity ◽  
High Sensitivity ◽  
Clinical Microbiology Laboratory ◽  
Isolation And Identification ◽  
E Coli ◽  
Extended Spectrum ◽  
Sensitivity Pattern

Infection that occurs in Indonesia has increased more significantly than before, compared to the increasing bacterial multidrug resistance (MDR) as the cause of infection. A study conducted in 5 hospitals in Indonesia in 2013 showed that the prevalence rate of extended-spectrum β-lactamase (ESBL)-producing bacteria reached 32-68%. The objective of this study is to detect the prevalence and resistence pattern of ESBL-producing Escherichia coli and Klebsiella pneumoniae in Dr. Zainoel Abidin General Hospital, Banda Aceh. This study was conducted from 1 September 2016 to 31 December 2016. Specimen types included in this study were blood, sputum, urine, pus, mucosal swab, and another body fluids sample. The sampling method in this study was total sampling that is all clinical specimen examined in Clinical Microbiology Laboratory. Isolation and identification ESBL-producing bacteria was performed by VITEK-2 machine (Biomerieux). The result of this study is that a total 122 E. coli and K. pneumoniae were isolated. That consisted of 48 (39%) E. coli isolates and 74 (61%) K. pneumoniae isolates. From 48 E. coli isolates it was found out that 41 (85%) had ESBL phenotypes and from 74 K. pneumoniae isolates it was found out that 59 (80%) had ESBL phenotypes. In total, 100 (82%) isolates from 122 isolates had ESBL phenotypes. Distribution of ESBL-producing E. coli and K. pneumoniae based on sample was 24 (89%) isolates from the total of 27 urine isolates, 18 (95%) isolates from the total of 19 blood isolates, 28 (78%) isolates from the total of 36 sputum isolates, and 30 (75%) isolates from the total of 40 pus isolates. Antibiotic sensitivity pattern of the E. coli and K. pneumoniae isolates had high sensitivity to amycasin dan meropenem which was above 89%. Meanwhile, it also had sensitivity to Fosfomycin and Piperacyclin-Tazobactam by 80% and 77% respectively. Another antibiotic was less effective

scholarly journals Isolation and identification of diarrheagenic Escherichia coli causing colibacillosis in calf in selective areas of Bangladesh

2014 ◽  
Vol 11 (2) ◽  
pp. 145-149 ◽  
Author(s):  
MK Hossain ◽  
M Rahman ◽  
A Nahar ◽  
A Khair ◽  
MM Alam
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Sensitivity ◽  
Veterinary Science ◽  
Isolation And Identification ◽  
Antibiotic Resistant ◽  
Fecal Samples ◽  
Biochemical Characteristics ◽  
E Coli ◽  
Sensitivity Pattern ◽  
Diarrheic Calves

This study was carried out to isolate, identify and characterize Escherichia coli from diarrheic calves, their prevalence and antibiotic sensitivity pattern during the period from November 2011 to December 2012. For this purpose 100 diarrheic fecal samples were collected from four different areas of Bangladesh. The samples were aseptically collected and brought to the laboratory of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh. The organisms were identified as E. coli on the basis of cultural, morphological and biochemical characteristics. Further the isolated bacteria were confirmed by amplification of E. coli specific 16sRNA gene. The antibiotic resistant pattern of the isolates was also performed. Out of 100 samples 49 (49%) were found to be positive for E. coli. The highest prevalence was found in Shahjadpur, Sirajganj (53.33%) while lowest was in BAU, Mymensingh (40%). The antibiogram study revealed that all isolates were resistant against ampicillin, erythromycin, azithromycin and streptomycin where erythromycin was 100% resistant, whereas 100% sensitivity was found to tetracycline and gentamicin which might be the best choice of drug for the treatment of diarrheagenic E. coli causing colibacillosis in calves.DOI: http://dx.doi.org/10.3329/bjvm.v11i2.19139Bangl. J. Vet. Med. (2013).11(2): 145-149

scholarly journals Recent Antibiotic Sensitivity Pattern of Escherichia Coli in Urinary Tract Infection

2018 ◽  
Vol 30 (1) ◽  
pp. 61-65
Author(s):  
Md Abdul Bari ◽  
Mashiur Arefin ◽  
Moriom Nessa ◽  
Golam Mostofa ◽  
Tafiqul Islam
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Urine Culture ◽  
Antibiotic Sensitivity ◽  
E Coli ◽  
Sensitivity Pattern ◽  
Marked Resistance ◽  
Culture Positive

The objective of the study is to assess the recent sensitivity pattern of Escherichia coli in Urinary tract infection (UTI). Widespread use of antibiotics has led to the emergence of resistant microorganisms. As the antibiotic sensitivity patterns of the microorganisms are frequently changing, this retrospective analysis was designed to assess the recent antibiotic sensitivity pattern of Escherichia coli (E. coli) in urinary tract infection among the human population. Details of 358 urine culture positive reports for E. coli and their antibiotic sensitivity pattern pertaining to the study period of 6 months from Jan 2017 to June 2017 were collected from Microbiology Laboratory of Diagnostic Centers at Rajshahi and the results were statistically analyzed. The antibiotics tested for sensitivity were amikacin, gentamycin, ciprofloxacin, levofloxacin, cotrimoxazole, nitrofurantoin, ceftazidime, ceftriaxone and cefixime. The sensitivity pattern of E.coli to antibiotics in UTI was amikacin (82.68 gentamycin (75.97%), nitrofurantoin (70.67), levofloxacin (44.13%), ceftazidime (42.17%), co-trimoxazole (40.78%), ceftriaxone (38.54%), ciprofloxacin (35.75%), and cefixime (24.02%). The study highlighted the marked resistance of E. coli to quinolones and third generation cephalosporins.TAJ 2017; 30(1): 61-65

scholarly journals Citric acid: A potential permeabilizer against multiple drug resistance enteropathogenic Escherichia coli

2012 ◽  
Vol 4 (1) ◽  
pp. 87-91 ◽  
Author(s):  
Preeti G. Dharmik ◽  
Ashok V. Gomashe ◽  
Bharat J. Wadher
Keyword(s):  
Escherichia Coli ◽  
Citric Acid ◽  
Antimicrobial Agents ◽  
Multiple Drug Resistance ◽  
Antibiotic Sensitivity ◽  
Multiple Drug ◽  
Enteric Infection ◽  
E Coli ◽  
Enteric Diseases ◽  
Sensitivity Pattern

Enteric diseases enter through the mouth and are usually spread by contaminated food, water or contact with contaminated vomit or feces. Enteric infection encompasses all the infections of the intestinal tract. These intestinal infections include organisms like Escherichia coli, Salmonella, Shigella, Klebsiella, Proteus etc. Out of these, E. coli are one of the common causes of enteric infection. In spite the introduction of a wide variety of antimicrobial agents against enteric diseases, life threatening infections caused by E. coli contributes to morbidity and mortality in patients. The present study was conducted to determine the antibiotic sensitivity pattern of E. coli obtained from stool samples and potentiation of antibiotic activity by citric acid against multiple drug resistant E. coli. Out of the 200 isolates of E. coli, 150 were found to be resistant to one or more antibiotics tested. 0.05% and 0.1% citric acid was found to be effective in increasing the potency of the all the antibiotics used in the study.

Molecular Characterization of Antibiotic Resistance Gene Pattern of Staphylococcus aureus and Escherichia coli in Mastitis Affected Dairy Cows

2020 ◽  
Author(s):  
T. Ramasamy ◽  
S. Keerthana ◽  
M.R. Srinivasan ◽  
D. Chandrasekar ◽  
K. Porteen ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Antimicrobial Resistance ◽  
Molecular Characterization ◽  
Antibiotic Sensitivity ◽  
Growth Media ◽  
E Coli ◽  
Sensitivity Pattern

Background: Antimicrobial resistance is one of the latest challenges facing the scientific community. Raising the drug resistance is caused mainly by indiscriminate usage of antibiotics in human and animal subjects and the spread of antibiotic resistance between the two has an emerging global threat. Hence, current study aimed to study the antimicrobial resistance pattern and molecular detection of antibiotic resistance genes in Staphylococcus aureus and Escherichia coli isolated from mastitis affected cows. Methods: Milk samples from mastitis affected cows were subjected to antibiotic sensitivity test and screened for presence of Staphylococcus aureus and Escherichia coli using differential growth media. Molecular characterization of Staphylococcus aureus and Escherichia coli was done with the help of PCR by amplification of ‘nuc’ and ‘uspA’ gene respectively. MICs of Penicillin and Tetracycline were determined using microdilution method. Result: Antibiotic sensitivity pattern for Penicillin G, Ampicillin, Amoxycillin, Cefotaxime, Ceftriaxone, Azithromycin, Ciprofloxacin, Gentamicin, Oxytetracycline, Tetracycline and Vancomycin were 74.19%, 100%, 93.50%, 61.29%, 29%, 35.48%, 9.70%, 9.70%, 70.96% and 70.96% respectively. More than 87.90% of the S. aureus and 50% of the E. coli isolated were resistant to â-lactam antibiotics while 75% of the E.coli and 65.70% of the S. aureus isolated were resistant to Tetracycline antibiotics. The MICs of Penicillin for S.aureus and E.coli are 26.88 µg/ml and 13.54 µg/ml respectively and the MICs of Tetracycline for S. aureus and E. coli are 243.75 µg/ml and 960.93 µg/ml respectively which is 8-9 folds higher than the standard MICs. From the present study, it can be inferred that bovine mastitis cases are highly resistant to antimicrobial drugs. Results further indicate that Staphylococcus aureus and Escherichia coli are both resistant to Penicillin and Tetracycline with very high MIC.

scholarly journals Prevalence, Characteristics and Antibiogram Profiles of Escherichia coli Isolated from Apparently Healthy Chickens in Mymensingh, Bangladesh

2013 ◽  
Vol 1 (1) ◽  
pp. 27-29 ◽  
Author(s):  
ATM Jakaria ◽  
Md Ariful Islam ◽  
Mst Minara Khatun
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Sensitivity ◽  
Blood Agar ◽  
Sensitivity Analyses ◽  
E Coli ◽  
Indigenous Chickens ◽  
Bright Pink ◽  
Sensitivity Pattern ◽  
Metallic Sheen ◽  
Apparently Healthy

Escherichia coli known to cause food-borne illnesses worldwide that are closely associated with the consumption of conta-minated poultry and egg products. This study was undertaken for cultural, biochemical and antibiotic sensitivity analyses of E. coli recovered from apparently healthy chickens. Cloacal samples (n=350) were aseptically collected from layers (n=150), broilers (n=150) and indigenous chickens (n=50). The samples were enriched in nutrient broth and streaked onto eosin methylene blue (EMB) agar, MacConkey (MC) agar, blood agar, salmonella-shigella (SS) agar and brilliant green agar (BGA) for cultural characterization of the E. coli isolates. Culture-positive samples yielded characteristic colonies of E. coli with metallic sheen on EMB agar, bright pink or red colonies on MC agar, hemolysis on blood agar, slight pink smooth colonies on SS agar and green color colonies on BGA media. The E. coli isolates produced acid and gas by ferm-enting sugars (dextrose, sucrose, lactose, maltose and mannitol) and gave positive reaction to indole, methyl red (MR) and catalase tests, but were negative to Voges-Proskauer (VP) test. The prevalence of E. coli in layers, broilers and indigenous chickens were 78.67, 82 and 70%, respectively. The antibiotic sensitivity pattern demonstrated that E. coli isolates were mostly sensitive to ciprofloxacin, gentamicin and cephalexin, and resistant to streptomycin, tetracycline, amoxicillin and nalidixic acid. Data of this study suggested that intestine of chicken could be a major reservoir of antibiotic resistant E. coli.DOI: http://dx.doi.org/10.3329/mh.v1i1.13710 Microbes and Health Vol.1(1) June 2012 pp.27-29

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