scholarly journals Isolation, identification, toxin profile and antibiogram of Escherichia coli isolated from broilers and layers in Mymensingh district of Bangladesh

1970 ◽  
Vol 6 (1) ◽  
pp. 1-5 ◽  
Author(s):  
MT Hossain ◽  
MP Siddique ◽  
FMA Hossain ◽  
MA Zinnah ◽  
MM Hossain ◽  
...  
Keyword(s):  
Antibiotic Sensitivity ◽  
Inoculation Test ◽  
Infant Mouse ◽  
E Coli ◽  
Heat Stable ◽  
Sensitivity Pattern ◽  
Highly Sensitive ◽  
Poultry Farms ◽  
Increasing Trend ◽  
Apparently Healthy

The study was conducted to isolate and identify E. coli from apparently healthy broilers and layers from different poultry farms adjacent to the Bangladesh Agricultural University, Mymensingh, Bangladesh, during the period of January to May 2006 and characterize their ability to produce enterotoxin and also the antibiogram of the isolates. A total of 110 fecal samples were collected from broiler (n=55) and layer (n=55) chickens. E. coli were isolated and identified by cultural, biochemical, motility test and the heat-stable toxins were determined by Infant Mouse Assay (IMA). In case of broilers, 35 (63.6%) samples were found positive while 31 (56.4%) from layers. The overall prevalence of E. coli was 60%. Among the isolates of E. coli, 22.86% isolates from broiler and 38.71% isolates from layer were found positive for their ability to produce enterotoxin based on mice inoculation test. The antibiotic sensitivity pattern showed that the isolates were highly sensitive to chloramphenicol, ciprofloxacin, kenamycin and cephalexin and an increasing trend of resistance was recorded in both broiler and layer isolates. It may be concluded from the results of this study that the high resistance of E. coli to antibiotics constitutes a threat to poultry industry in Bangladesh.  Key words: E. coli, toxin, antibiogram DOI = 10.3329/bjvm.v6i1.1330 Bangl. J. Vet. Med. (2008). 6 (1): 01-05

scholarly journals Characterization and Antibiogram of Escherichia coli Associated with Mortality in Broilers and Ducklings in Bangladesh

1970 ◽  
Vol 2 (1) ◽  
pp. 9-14 ◽  
Author(s):  
MT Islam ◽  
MA Islam ◽  
MA Samad ◽  
SML Kabir
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Sensitivity ◽  
High Rate ◽  
E Coli ◽  
Sensitivity Pattern ◽  
Highly Sensitive ◽  
Biochemical Studies ◽  
Increasing Trend ◽  
Escherichia Coli Infection ◽  
Hemorrhagic Enteritis

Mortality in broilers (6.56%) and growing ducks (11.0%) caused by Escherichia coli was recorded in the experimental flocks study during the period from May to August 2003. E. coli organisms isolated from broiler birds affected with characteristic lesions of omphalitis and yolk sac infection, fibrinous pericarditis and peri-hepatitis , hemorrhagic enteritis, and accumulation of excessive pericardial and peritoneal fluid, whereas from ducks with lesions of hemorrhagic enteritis and extensive epicardial hemorrhages. Each of the 21 isolates collected from broilers and 11 isolates from ducks was characterized by cultural and biochemical studies, of which 8 isolates from broilers and 5 isolates from ducks were tested for antibiotic sensitivity with 9 different antibiotics. The antibiotic sensitivity pattern showed that the isolates were highly sensitive to ciprofloxacin but an increasing trend of resistance was recorded in broilers (7 / 9) than duck (4 / 9) isolates. It may be concluded from the results of this study that the high rate of E. coli infection in broilers and ducks along with the high resistance of isolates to antibiotics constitute a threat to the poultry industry in Bangladesh. Key words: Escherichia coli infection; mortality; broilers; ducks; characterization; antibiogram doi: 10.3329/bjvm.v2i1.1927 Bangl. J. Vet. Med. (2004). 2 (1) : 09-14

scholarly journals ISOLATION AND CHARACTERIZATION OF ESCHERICHIA COLI FROM BUFFALO CALVES IN SOME SELECTED AREAS OF BANGLADESH

1970 ◽  
Vol 8 (1) ◽  
pp. 23-26 ◽  
Author(s):  
SK Paul ◽  
MSR Khan ◽  
MA Rashid ◽  
J Hassan ◽  
SMS Mahmud
Keyword(s):  
Escherichia Coli ◽  
Nalidixic Acid ◽  
Rectal Swab ◽  
Antibiotic Sensitivity ◽  
Buffalo Calves ◽  
E Coli ◽  
Isolation And Characterization ◽  
Sensitivity Pattern ◽  
Highly Sensitive

The research works was conducted with a view to isolate and identify the Escherichia coli (E. coli) organism from diarrhoeic cases of buffalo reared in selected areas of Bangladesh as well the prevalence and antibiotic sensitivity pattern of the isolated E. coli in the Department of Microbiology and Hygiene, Bangladesh Agricultural University (BAU), Mymensingh-2202 during the period from April 2008 to May 2009. A total of 50 rectal swab samples were collected from 4 different places namely Haluaghat and Boira of Mymensingh, Madupur of Tangail and Kazipur of Sirajgonj districts. The samples were aseptically carried to the laboratory of the Department of Microbiology and Hygiene and subjected to different cultural, morphological and biochemical examinations. Upon cultural, morphological and biochemical examinations 23 (45%) samples were found to be positive for E. coli. The highest prevalence was found in Haluaghat, Mymensingh (53.33%) and the lowest (40.00%) in Boira, Mymensingh and Kazipur, Sirajganj. Antibiogram study revealed that the isolated E. coli was highly sensitive to Enrofloxacin and Ciprofloxacin, moderately sensitive to Cefalexin and Amoxicillin, and resistant to Nalidixic acid and Erythromycin. DOI = 10.3329/bjvm.v8i1.7398 Bangl. J. Vet. Med. (2010). 8(1): 23-26

scholarly journals Prevalence, Characteristics and Antibiogram Profiles of Escherichia coli Isolated from Apparently Healthy Chickens in Mymensingh, Bangladesh

2013 ◽  
Vol 1 (1) ◽  
pp. 27-29 ◽  
Author(s):  
ATM Jakaria ◽  
Md Ariful Islam ◽  
Mst Minara Khatun
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Sensitivity ◽  
Blood Agar ◽  
Sensitivity Analyses ◽  
E Coli ◽  
Indigenous Chickens ◽  
Bright Pink ◽  
Sensitivity Pattern ◽  
Metallic Sheen ◽  
Apparently Healthy

Escherichia coli known to cause food-borne illnesses worldwide that are closely associated with the consumption of conta-minated poultry and egg products. This study was undertaken for cultural, biochemical and antibiotic sensitivity analyses of E. coli recovered from apparently healthy chickens. Cloacal samples (n=350) were aseptically collected from layers (n=150), broilers (n=150) and indigenous chickens (n=50). The samples were enriched in nutrient broth and streaked onto eosin methylene blue (EMB) agar, MacConkey (MC) agar, blood agar, salmonella-shigella (SS) agar and brilliant green agar (BGA) for cultural characterization of the E. coli isolates. Culture-positive samples yielded characteristic colonies of E. coli with metallic sheen on EMB agar, bright pink or red colonies on MC agar, hemolysis on blood agar, slight pink smooth colonies on SS agar and green color colonies on BGA media. The E. coli isolates produced acid and gas by ferm-enting sugars (dextrose, sucrose, lactose, maltose and mannitol) and gave positive reaction to indole, methyl red (MR) and catalase tests, but were negative to Voges-Proskauer (VP) test. The prevalence of E. coli in layers, broilers and indigenous chickens were 78.67, 82 and 70%, respectively. The antibiotic sensitivity pattern demonstrated that E. coli isolates were mostly sensitive to ciprofloxacin, gentamicin and cephalexin, and resistant to streptomycin, tetracycline, amoxicillin and nalidixic acid. Data of this study suggested that intestine of chicken could be a major reservoir of antibiotic resistant E. coli.DOI: http://dx.doi.org/10.3329/mh.v1i1.13710 Microbes and Health Vol.1(1) June 2012 pp.27-29

scholarly journals Haemato-biochemical Changes and Antibiotic Sensitivity to Eschericia coli Associated with Concurrent Enteric and Septicaemic Infection in Calves

1970 ◽  
Vol 1 (1) ◽  
pp. 39-43 ◽  
Author(s):  
MA Samad ◽  
MA Islam ◽  
KA Hossain ◽  
MT Islam ◽  
S Saha
Keyword(s):  
Escherichia Coli ◽  
Serum Magnesium ◽  
Antibiotic Sensitivity ◽  
Blood Samples ◽  
E Coli ◽  
Drug Of Choice ◽  
Sensitivity Pattern ◽  
Highly Sensitive ◽  
Escherichia Coli Infection ◽  
First Time

Concurrent enteric and septicaemic Escherichia coli infection was recorded in six local calves aged between 8 to 12 months old for the first time in Bangladesh during the period from May to June 2002. Bacteriologic examination of both the faecal and blood samples of six febrile calves showed positive for E. coli of all the six faecal (100%) and only two blood (33.33%) samples. Clinical examination revealed significantly (p < 0.01) high rectal temperature at peak febrile stage which varied from 103.5 to 106°F with a mean of 104.7 ± 0.85°F. The fever associated signs and clinical anaemia were recorded in all these calves. Haematological examination showed significantly (p < 0.01) decreased haemoglobin (5.58 ± 0.45 g%), packed cell volume (19.83 ± 1.95 %), total erythrocytic count (3.52 ± 0.58  106/ mm3) and total leukocytic count (6.68 ± 0.44  103 / mm3) which indicates anaemia and leukopenia at the peak febrile stage of the disease. Biochemical studies showed significantly (p < 0.05) increased that serum magnesium and iron values were at fever stage, whereas serum phosphorus significantly (p < 0.01) decreased at the 4th day (convalescent stage) but no significant (p > 0.05) change was observed with the serum calcium, sodium, potassium and chloride values between prefever and fever stage. Antibiotic sensitivity pattern of E. coli isolated from faecal and blood samples revealed that these isolates were found to be resistant to penicillin, ampicillin and amoxycillin, moderately sensitive to gentamicin and streptomycin but highly sensitive to tetracycline. This indicates that tetracycline could be the drug of choice for both the septicaemic and enteric E. coli infection in calves. Key words: Haematobiochemical; Escherichia coli; enteric; septicemic; infection; calves DOI = 10.3329/bjvm.v1i1.1916 Bangl. J. Vet. Med.  (2003). 1(1) : 39 - 43

scholarly journals Isolation, identification and characterization of Salmonella from duck

1970 ◽  
Vol 6 (1) ◽  
pp. 7-12 ◽  
Author(s):  
T Mondal ◽  
MSR Khan ◽  
M Alam ◽  
M Purakayastha ◽  
M Das ◽  
...  
Keyword(s):  
Sensitivity Analysis ◽  
Key Words ◽  
Nalidixic Acid ◽  
Antibiotic Sensitivity ◽  
Poultry Farm ◽  
Sensitivity Pattern ◽  
Highly Sensitive ◽  
Identification And Characterization ◽  
Apparently Healthy

The present study was performed with the aim to isolate and identify Salmonella organism from diarrhoeic and apparently healthy ducks and to characterize duck Salmonella by biochemical test. Antibiotic sensitivity analysis of duck Salmonella was performed. A total of 65 cloacal samples were collected from ducks of three different regions such as Char Nilokkhiya, BAU Poultry Farm and Boyra, Mymensingh. Out of 65 samples 9 (13.07%) were found positive. The antibiotic sensitivity pattern showed that the duck isolates were highly sensitive to ciprofloxacin, kanamycin, nalidixic acid, co-trimoxazole and cephalexin but these isolates were highly resistant to chloramphenicol. Key words: Salmonella, duck, isolation, identification, characterization DOI = 10.3329/bjvm.v6i1.1331 Bangl. J. Vet. Med. (2008). 6 (1): 07-12

scholarly journals Isolation and Detection of Antibiotic Sensitivity Pattern of Escherichia coli from Ducks in Bangladesh and Nepal

2013 ◽  
Vol 1 (1) ◽  
pp. 6-8
Author(s):  
Avishek Singh ◽  
Md Shahidur Rahman Khan ◽  
Sukumar Saha ◽  
Jayedul Hassan ◽  
Urmi Roy
Keyword(s):  
Escherichia Coli ◽  
Nalidixic Acid ◽  
Antibiotic Sensitivity ◽  
E Coli ◽  
Sensitivity Pattern ◽  
Highly Sensitive ◽  
Resistance Patterns ◽  
Typical Lesion ◽  
Different Origins ◽  
Biochemical Examination

A total of 120 rectal swab samples from ducks (sixty samples from Nepalgunj area of Nepal and 60 from Boyera area under Mymensingh district of Bangladesh) were collected for the isolation of Escherichia coli and their antibiogram study. After cultural and biochemical examination, a total of 40 samples from Nepalgunj and 45 samples from Boyera were found positive for E. coli. Pathogenicity study of 10 positive isolates from Nepalgunj and 12 positive isolates from Boyera were done to detect the presence of enterotoxin. All inoculated mice died showing typical lesion of extensive hemorrhage and massive edema. The isolates from two different origins showed major difference in their antibiogram study. The isolates of Nepal were highly sensitive to ciprofloxacin, co-trimoxazole, chloramphenicol and amoxicillin; moderately sensitive to nalidixic acid and; less sensitive to kanamycin and resistant to cephalexin. However, the isolates of Bangladesh were highly sensitive to ciprofloxacin, chloramphenicol and amoxicillin; moderately sensitive to nalidixic acid, cephalexin, and co-trimoxazole; less sensitive to kanamycin. This variation of antibiotic sensitivity and resistance patterns among the E. coli isolates of Nepal and Bangladesh might be due to strain variations and indiscriminate use of antibiotics in these two different countries.DOI: http://dx.doi.org/10.3329/mh.v1i1.13704 Microbes and Health Vol.1(1) June 2012 pp.6-8

scholarly journals Blood Culture Isolates and Antibiogram of Salmonella: The Gathering Storm

KYAMC Journal ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 188-190
Author(s):  
Ishrat Binte Reza ◽  
HAM Nazmul Ahasan ◽  
Moshtaque Ahmed ◽  
Homayra Tahseen ◽  
Tasmina Chowdhury
Keyword(s):  
Drug Resistance ◽  
Antibiotic Sensitivity ◽  
Multidrug Resistant ◽  
Bacterial Strains ◽  
College Hospital ◽  
Major Health ◽  
Medical College ◽  
Sensitivity Pattern ◽  
Highly Sensitive ◽  
The World

Introduction: Typhoid fever is a common problem all over the world including Bangladesh. It is caused by salmonella typhae and paratyphae and it is an old infectious water born disease. It is encountered in Bangladesh throughout the year. For many years it is treated by Chloramphenicol and subsequently Cotrimoxazole and Amoxicillin. Despite the use of newly developed antibacterial drugs, enteric fevers caused by multidrug-resistant bacterial strains are one of major health problems in Bangladesh. Multidrug resistant Salmonella sp. has been emerged is a cause of concern. Materials and Methods: This is a retrospective study done at the Popular Medical College Hospital over a period of 6 months between January 2018 to june 2018. Results: During the study period, total 115 cases were enrolled. Regarding antibiotic sensitivity pattern, 69% strains were sensitive to Ampicillin, 100% to Cefixime and Ceftriaxone, 75% to Cotrimoxazole, 52% to Nalidixic Acid and 68% to Ciprofloxacin, 69% to Levofloxacin, 80% to Cholarphenicol. Conclusion: Ceftriaxone and Cefixime were found to be highly sensitive. So, we should be very careful for the judicious use of these valuable drug to prevent drug resistance. KYAMC Journal Vol. 10, No.-4, January 2020, Page 188-190

scholarly journals Bacterial Etiology and Antibiotic Sensitivity Pattern of Community Acquired Pneumonia in Diabetic Patients: Experience in a Tertiary Care Hospital in Bangladesh

2017 ◽  
Vol 7 (2) ◽  
pp. 101-105
Author(s):  
Jamal Uddin Ahmed ◽  
Mohammad Delwar Hossain ◽  
Muhammad Abdur Rahim ◽  
Farhana Afroz ◽  
AKM Musa
Keyword(s):  
Staphylococcus Aureus ◽  
Klebsiella Pneumoniae ◽  
Antibiotic Sensitivity ◽  
Community Acquired Pneumonia ◽  
Sputum Culture ◽  
Diabetic Patients ◽  
Care Hospital ◽  
E Coli ◽  
Sensitivity Pattern ◽  
Pseudomonas Species

Background: Diabetes mellitus (DM) is an immunosuppressive condition and uncontrolled diabetes is associated with increased susceptibility to various infections like pneumonia. Community acquired pneumonia (CAP) in diabetic patients is often caused by more virulent or atypical organisms and associated with increased resistance to conventional antibiotics. The aims of this study were to identify the bacterial etiology of CAP in patients with DM and to see their antibiotic sensitivity pattern.Methods: This was a cross-sectional, observational study conducted in the Department of Internal Medicine & Pulmonology of BIRDEM General Hospital, Dhaka, Bangladesh, from January 2013 to December 2015. A total of 120 hospitalized diabetic patients diagnosed with CAP and with a positive sputum culture growth of any bacteria were included in the study.Results: Majority (67%) of the patients were male. Mean age of the patients was - 55.69 ±10.5 years. Mean duration of diabetes was - 7.35 ±1.3 years. Mean HbA1c was - 8.6 ±1.89%. Sputum for culture showed that out of 120 (100%) patients, Klebsiella pneumoniae was detected in 53 (44.2%) patients, Staphylococcus aureus in 18 (15.0%), Pseudomonas species in 16 (13.3%) patients, Acinetobacter in 10 (8.3%), Escherichia coli in 9 (7.5%) patients and 14 (11.7%) patients had growth of other organisms. Sensitivity pattern of different bacterial growth in sputum to commonly used antibiotics like ceftriaxone, ciprofloxacin, amikacin and imipenem were as follows – Klebsiella (19%, 47%, 74%, 96% respectively), Staph aureus (11%, 33%, 78%, 67% respectively), Pseudomonas (19%, 75%, 81%, 88% respectively), Acinetobacter (0%, 0%, 20%, 50% respectively), E. coli (22%, 22%, 100%, 100% respectively). All (100%) of the Pseudomonas and Acinetobacter were sensitive to colistin. Most of the growth of all the bacteria (Klebsiella 94%, Staphylococcus 78%, Pseudomonas 81%, Acinetobacter 100%, E. coli 100%) occurred in patients with poor glycemic control (HbA1c ? 7.0%).Conclusion: This study results suggest that CAP in diabetic patients are more frequently due to Klebsiella pneumoniae, Staphylococcus aureus and Pseudomonas species and mostly they are less sensitive to commonly used antibiotics like ceftriaxone and ciprofloxacin. So, whenever possible, treatment of CAP should be guided by sputum culture and sensitivity test and for empirical treatment of CAP in diabetic patients, alternative antibiotics like imipenem and amikacin should be considered.Birdem Med J 2017; 7(2): 101-105

scholarly journals ISOLATION AND IDENTIFICATION OF MICROFLORA FROM APPARENTLY HEALTHY CAGED PARROTS OF DHAKA ZOO OF BANGLADESH

1970 ◽  
Vol 8 (1) ◽  
pp. 05-10 ◽  
Author(s):  
J Akhter ◽  
MT Hossain ◽  
MT Islam ◽  
MP Siddique ◽  
MA Islam
Keyword(s):  
Research Work ◽  
Antibiotic Sensitivity ◽  
Biochemical Properties ◽  
Gram Negative Bacteria ◽  
Bacterial Isolates ◽  
Salmonella Spp ◽  
Isolation And Identification ◽  
Gram Negative ◽  
E Coli ◽  
Apparently Healthy

The research work was conducted to isolate and identify the microflora from apparently healthy caged parrots. A total of 45 samples (oral swabs, cloacal swabs and feces) were collected from five types of caged parrots (Gray cockatiels, Rose ringed parakeet, Alexandriane parakeet, Red breast parakeet and Blossom headed parakeet) of Dhaka Zoo during the period from April to August 2009. The samples were cultured on different bacteriological media and the bacteria were identified by their cultural and biochemical properties. All the isolates were allowed for antibiogram study. The bacteria isolated in this study from different types of caged parrots were E. coli (64.44%), Salmonella spp. (46.67%), Staphylococcus spp. (46.67%), Pasteurella spp. (33.33%), Proteus spp. (6.67%) and some unidentified Gram-positive and Gram-negative bacteria. Of these isolates, E. coli was the most frequent isolate. The frequency of Gram-negative bacteria was higher in this study. The percentage of bacterial isolates recovered from each type of parrots was almost similar. Irrespective of types of parrots, the higher percentage of different bacteria was isolated from cloacal swab (77.78%) followed by feces (75.56%). The 68.89% isolates were recovered from oral swab. All the suspected isolates of Salmonella spp. were confirmed by slide agglutination test using Salmonella polyvalent ‘O’ antiserum. Among the 21 Salmonella spp. isolated in this study, 4 (19.05%) isolates were identified as S. Pullorum when tested with specific antisera against S. Pullorum. The results of antibiotic sensitivity tests revealed that ampicillin and amoxicillin were completely resistant to E. coli and Pasteurella spp.; ampicillin to Proteus spp.; and furazolidone to Salmonella spp. and Pasteurella spp. However, the antibiotics of fluoroquinolone group such as ciprofloxacin, norfloxacin and enrofloxacin showed moderate to high sensitivity against almost all the bacterial isolates. Of these, ciprofloxacin was found to be consistently highly sensitive to all the bacterial isolates. DOI = 10.3329/bjvm.v8i1.8349 Bangl. J. Vet. Med. (2010). 8(1): 05-10

scholarly journals Isolation Of Extended-Spectrum B-Lactamase (ESBL) Producing Escherichia coli and Klebsiella pneumiae From Dr. Zainoel Abidin General Hospital, Aceh

2019 ◽  
Vol 4 (1) ◽  
pp. 16-22 ◽  
Author(s):  
Zinatul Hayati ◽  
Syamsul Rizal ◽  
Ridhia Putri
Keyword(s):  
Escherichia Coli ◽  
General Hospital ◽  
Clinical Specimen ◽  
Antibiotic Sensitivity ◽  
High Sensitivity ◽  
Clinical Microbiology Laboratory ◽  
Isolation And Identification ◽  
E Coli ◽  
Extended Spectrum ◽  
Sensitivity Pattern

Infection that occurs in Indonesia has increased more significantly than before, compared to the increasing bacterial multidrug resistance (MDR) as the cause of infection. A study conducted in 5 hospitals in Indonesia in 2013 showed that the prevalence rate of extended-spectrum β-lactamase (ESBL)-producing bacteria reached 32-68%. The objective of this study is to detect the prevalence and resistence pattern of ESBL-producing Escherichia coli and Klebsiella pneumoniae in Dr. Zainoel Abidin General Hospital, Banda Aceh. This study was conducted from 1 September 2016 to 31 December 2016. Specimen types included in this study were blood, sputum, urine, pus, mucosal swab, and another body fluids sample. The sampling method in this study was total sampling that is all clinical specimen examined in Clinical Microbiology Laboratory. Isolation and identification ESBL-producing bacteria was performed by VITEK-2 machine (Biomerieux). The result of this study is that a total 122 E. coli and K. pneumoniae were isolated. That consisted of 48 (39%) E. coli isolates and 74 (61%) K. pneumoniae isolates. From 48 E. coli isolates it was found out that 41 (85%) had ESBL phenotypes and from 74 K. pneumoniae isolates it was found out that 59 (80%) had ESBL phenotypes. In total, 100 (82%) isolates from 122 isolates had ESBL phenotypes. Distribution of ESBL-producing E. coli and K. pneumoniae based on sample was 24 (89%) isolates from the total of 27 urine isolates, 18 (95%) isolates from the total of 19 blood isolates, 28 (78%) isolates from the total of 36 sputum isolates, and 30 (75%) isolates from the total of 40 pus isolates. Antibiotic sensitivity pattern of the E. coli and K. pneumoniae isolates had high sensitivity to amycasin dan meropenem which was above 89%. Meanwhile, it also had sensitivity to Fosfomycin and Piperacyclin-Tazobactam by 80% and 77% respectively. Another antibiotic was less effective

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