scholarly journals Isolation and identification of diarrheagenic Escherichia coli causing colibacillosis in calf in selective areas of Bangladesh

2014 ◽  
Vol 11 (2) ◽  
pp. 145-149 ◽  
Author(s):  
MK Hossain ◽  
M Rahman ◽  
A Nahar ◽  
A Khair ◽  
MM Alam
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Sensitivity ◽  
Veterinary Science ◽  
Isolation And Identification ◽  
Antibiotic Resistant ◽  
Fecal Samples ◽  
Biochemical Characteristics ◽  
E Coli ◽  
Sensitivity Pattern ◽  
Diarrheic Calves

This study was carried out to isolate, identify and characterize Escherichia coli from diarrheic calves, their prevalence and antibiotic sensitivity pattern during the period from November 2011 to December 2012. For this purpose 100 diarrheic fecal samples were collected from four different areas of Bangladesh. The samples were aseptically collected and brought to the laboratory of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh. The organisms were identified as E. coli on the basis of cultural, morphological and biochemical characteristics. Further the isolated bacteria were confirmed by amplification of E. coli specific 16sRNA gene. The antibiotic resistant pattern of the isolates was also performed. Out of 100 samples 49 (49%) were found to be positive for E. coli. The highest prevalence was found in Shahjadpur, Sirajganj (53.33%) while lowest was in BAU, Mymensingh (40%). The antibiogram study revealed that all isolates were resistant against ampicillin, erythromycin, azithromycin and streptomycin where erythromycin was 100% resistant, whereas 100% sensitivity was found to tetracycline and gentamicin which might be the best choice of drug for the treatment of diarrheagenic E. coli causing colibacillosis in calves.DOI: http://dx.doi.org/10.3329/bjvm.v11i2.19139Bangl. J. Vet. Med. (2013).11(2): 145-149

scholarly journals Isolation Of Extended-Spectrum B-Lactamase (ESBL) Producing Escherichia coli and Klebsiella pneumiae From Dr. Zainoel Abidin General Hospital, Aceh

2019 ◽  
Vol 4 (1) ◽  
pp. 16-22 ◽  
Author(s):  
Zinatul Hayati ◽  
Syamsul Rizal ◽  
Ridhia Putri
Keyword(s):  
Escherichia Coli ◽  
General Hospital ◽  
Clinical Specimen ◽  
Antibiotic Sensitivity ◽  
High Sensitivity ◽  
Clinical Microbiology Laboratory ◽  
Isolation And Identification ◽  
E Coli ◽  
Extended Spectrum ◽  
Sensitivity Pattern

Infection that occurs in Indonesia has increased more significantly than before, compared to the increasing bacterial multidrug resistance (MDR) as the cause of infection. A study conducted in 5 hospitals in Indonesia in 2013 showed that the prevalence rate of extended-spectrum β-lactamase (ESBL)-producing bacteria reached 32-68%. The objective of this study is to detect the prevalence and resistence pattern of ESBL-producing Escherichia coli and Klebsiella pneumoniae in Dr. Zainoel Abidin General Hospital, Banda Aceh. This study was conducted from 1 September 2016 to 31 December 2016. Specimen types included in this study were blood, sputum, urine, pus, mucosal swab, and another body fluids sample. The sampling method in this study was total sampling that is all clinical specimen examined in Clinical Microbiology Laboratory. Isolation and identification ESBL-producing bacteria was performed by VITEK-2 machine (Biomerieux). The result of this study is that a total 122 E. coli and K. pneumoniae were isolated. That consisted of 48 (39%) E. coli isolates and 74 (61%) K. pneumoniae isolates. From 48 E. coli isolates it was found out that 41 (85%) had ESBL phenotypes and from 74 K. pneumoniae isolates it was found out that 59 (80%) had ESBL phenotypes. In total, 100 (82%) isolates from 122 isolates had ESBL phenotypes. Distribution of ESBL-producing E. coli and K. pneumoniae based on sample was 24 (89%) isolates from the total of 27 urine isolates, 18 (95%) isolates from the total of 19 blood isolates, 28 (78%) isolates from the total of 36 sputum isolates, and 30 (75%) isolates from the total of 40 pus isolates. Antibiotic sensitivity pattern of the E. coli and K. pneumoniae isolates had high sensitivity to amycasin dan meropenem which was above 89%. Meanwhile, it also had sensitivity to Fosfomycin and Piperacyclin-Tazobactam by 80% and 77% respectively. Another antibiotic was less effective

scholarly journals Prevalence of Multidrug Resistant Escherichia Coli in Pigeon in Mymensingh, Bangladesh

2014 ◽  
Vol 2 (1) ◽  
pp. 5-7 ◽  
Author(s):  
Rubel Kanti Dey ◽  
Mst Minara Khatun ◽  
Md Ariful Islam ◽  
Md Sahadat Hosain
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Sensitivity ◽  
Multidrug Resistant ◽  
Drug Resistant ◽  
Bacterial Isolates ◽  
Macconkey Agar ◽  
Biochemical Characteristics ◽  
E Coli ◽  
Sensitivity Pattern ◽  
Hygienic Measures

To determine prevalence of antimicrobial resistant Escherichia coli in pigeon a total of 112 samples such as cloacal swabs (n=36), foot pads (n=36) and feces (n=40) were collected from pigeon aseptically. Samples were enriched in nutrient broth and then streaked onto eosine methylene blue agar, Salmonella-Shigella agar, MacConkey agar and blood agar. The cultural and biochemical characteristics of bacterial isolates of pigeon were indicative of E. coli. The prevalence of E. coli in cloacal swabs, foot pads and feces samples were 86.11%, 44.44% and 77.50%, respectively. The overall prevalence of E. coli in pigeon was 69.64% (78 of 122 samples were found positive for E. coli). The antibiotic sensitivity pattern showed that E. coli isolates were sensitive to erythromycin, ciprofloxacin, kanamycin, nalidixic acid and resistant to amoxicillin, tetracycline and sulphamethaxazole. It may be concluded that pigeons from Mymensingh locality in Bangladesh carry multidrug resistant E. coli. Data of this study will be helpful in creating public health awareness among people and help controlling transmission of drug resistant E. coli by undertaking effective sanitary and hygienic measures. Microbes and Health, June 2013, 2(1): 5-7DOI: http://dx.doi.org/10.3329/mh.v2i1.17254

scholarly journals Characterization and Antibiogram of Escherichia coli Associated with Mortality in Broilers and Ducklings in Bangladesh

1970 ◽  
Vol 2 (1) ◽  
pp. 9-14 ◽  
Author(s):  
MT Islam ◽  
MA Islam ◽  
MA Samad ◽  
SML Kabir
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Sensitivity ◽  
High Rate ◽  
E Coli ◽  
Sensitivity Pattern ◽  
Highly Sensitive ◽  
Biochemical Studies ◽  
Increasing Trend ◽  
Escherichia Coli Infection ◽  
Hemorrhagic Enteritis

Mortality in broilers (6.56%) and growing ducks (11.0%) caused by Escherichia coli was recorded in the experimental flocks study during the period from May to August 2003. E. coli organisms isolated from broiler birds affected with characteristic lesions of omphalitis and yolk sac infection, fibrinous pericarditis and peri-hepatitis , hemorrhagic enteritis, and accumulation of excessive pericardial and peritoneal fluid, whereas from ducks with lesions of hemorrhagic enteritis and extensive epicardial hemorrhages. Each of the 21 isolates collected from broilers and 11 isolates from ducks was characterized by cultural and biochemical studies, of which 8 isolates from broilers and 5 isolates from ducks were tested for antibiotic sensitivity with 9 different antibiotics. The antibiotic sensitivity pattern showed that the isolates were highly sensitive to ciprofloxacin but an increasing trend of resistance was recorded in broilers (7 / 9) than duck (4 / 9) isolates. It may be concluded from the results of this study that the high rate of E. coli infection in broilers and ducks along with the high resistance of isolates to antibiotics constitute a threat to the poultry industry in Bangladesh. Key words: Escherichia coli infection; mortality; broilers; ducks; characterization; antibiogram doi: 10.3329/bjvm.v2i1.1927 Bangl. J. Vet. Med. (2004). 2 (1) : 09-14

scholarly journals ISOLATION AND CHARACTERIZATION OF ESCHERICHIA COLI FROM BUFFALO CALVES IN SOME SELECTED AREAS OF BANGLADESH

1970 ◽  
Vol 8 (1) ◽  
pp. 23-26 ◽  
Author(s):  
SK Paul ◽  
MSR Khan ◽  
MA Rashid ◽  
J Hassan ◽  
SMS Mahmud
Keyword(s):  
Escherichia Coli ◽  
Nalidixic Acid ◽  
Rectal Swab ◽  
Antibiotic Sensitivity ◽  
Buffalo Calves ◽  
E Coli ◽  
Isolation And Characterization ◽  
Sensitivity Pattern ◽  
Highly Sensitive

The research works was conducted with a view to isolate and identify the Escherichia coli (E. coli) organism from diarrhoeic cases of buffalo reared in selected areas of Bangladesh as well the prevalence and antibiotic sensitivity pattern of the isolated E. coli in the Department of Microbiology and Hygiene, Bangladesh Agricultural University (BAU), Mymensingh-2202 during the period from April 2008 to May 2009. A total of 50 rectal swab samples were collected from 4 different places namely Haluaghat and Boira of Mymensingh, Madupur of Tangail and Kazipur of Sirajgonj districts. The samples were aseptically carried to the laboratory of the Department of Microbiology and Hygiene and subjected to different cultural, morphological and biochemical examinations. Upon cultural, morphological and biochemical examinations 23 (45%) samples were found to be positive for E. coli. The highest prevalence was found in Haluaghat, Mymensingh (53.33%) and the lowest (40.00%) in Boira, Mymensingh and Kazipur, Sirajganj. Antibiogram study revealed that the isolated E. coli was highly sensitive to Enrofloxacin and Ciprofloxacin, moderately sensitive to Cefalexin and Amoxicillin, and resistant to Nalidixic acid and Erythromycin. DOI = 10.3329/bjvm.v8i1.7398 Bangl. J. Vet. Med. (2010). 8(1): 23-26

Molecular detection of multidrug and colistin-resistant Escherichia coli isolated from house flies in various environmental settings

2019 ◽  
Vol 14 (10) ◽  
pp. 847-858 ◽  
Author(s):  
Abdus Sobur ◽  
Zobayda F Haque ◽  
Abdullah AM Sabuj ◽  
Samina Ievy ◽  
AMM Taufiqur Rahman ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Multidrug Resistant ◽  
Diffusion Test ◽  
Isolation And Identification ◽  
Antibiotic Resistant ◽  
Disk Diffusion Test ◽  
House Flies ◽  
E Coli ◽  
Pathogenic Escherichia Coli ◽  
Antimicrobial Resistance Genes

Aim: To detect antibiotic-resistant pathogenic Escherichia coli in house flies captured from various environmental settings. Materials & methods: Isolation and identification of E. coli was performed based on culture and PCR. Antimicrobial susceptibility testing was conducted using disk diffusion test. Detection of virulence and antimicrobial resistance genes was carried out using PCR methods. Results: The prevalence of E. coli in flies was 61% with highest in dairy farms (70.67%) followed by food centers (65.33%), dustbins (64%) and area close to hospital (44%). The prevalence rates of tetA, tetB, ereA, CITM, SHV, mcr-3 and catA1 resistance determinants were 88.75, 48.52, 41.67, 37.17, 27.77, 18.37 and 14.81%, respectively. Conclusion: Multidrug-resistant pathogenic E. coli are widely distributed in house flies, which requires a one-health approach to delineate their control.

scholarly journals Investigation of Pathogenic Escherichia Coli from Diarrheic Calves in Selective Area of Bangladesh

2015 ◽  
Vol 13 (1) ◽  
pp. 45-51 ◽  
Author(s):  
AKMA Islam ◽  
M Rahman ◽  
A Nahar ◽  
A Khair ◽  
MM Alam
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Agents ◽  
Biochemical Properties ◽  
Molecular Techniques ◽  
Diffusion Method ◽  
Molecular Technique ◽  
Isolation And Identification ◽  
Disk Diffusion Method ◽  
E Coli ◽  
Diarrheic Calves

Molecular technique was used to investigate the prevalence of virulent diarrheic genes in pathogenic Escherichia coli and their antibiotic sensitivity patterns. A hundred samples from 100 different diarrheic calves from mid-north-western part of Bangladesh were screened for the presence of virulence factors associated with diarrhea. Following isolation and identification on the basis of cultural, morphological and biochemical properties, the presence of the virulence genes such as eaeA, bfpA, elt, est, stx1 and stx2 were examined using PCR. Antimicrobial susceptibility of 57 E. coli was determined by agar disk diffusion method for 8 antimicrobial agents. Out of 100 samples 57 (57%) were found to be positive for E. coli and their distribution rates according to their age, breed and sex were  66.7% ( 6 days old ), 85.7% (Sahiwal breed) and in  64.2 % (female calves) respectively. Among 57 E. coli isolates, only 16 isolates were analyzed for the detection of the said genes. Among them, only eaeA gene was detected in 2 E. coli isolates (12.5 %). Antibiotic resistance patterns revealed that Oxacillin, Rifampicin and Penicillin were  100% resistant followed by Erythromycin which was more than 80% resistant. In case of Amoxicillin and Tetracycline, about 59.65% and 61.40% were found to be resistant respectively whereas all 57 E. coli isolates showed moderately susceptible (30%) to Cefuroxime, a second generation Cephalosporin. Therefore, none of the eight antimicrobials studied can not be recommended as single best therapeutic agent for the treatment of neonatal calf diarrhea. In addition, this study indicated that diarrhea in calves in these locations can be ascribed to mainly Enteropathogenic E. coli (EPEC) which was atypical (only contained the eaeA genes but not bfpA). However, further studies are necessary to characterize the isolated eaeA gene positive E. coli by serotyping, tissue culture assay and other molecular techniques to find out the potentiality of those virulent genes contributing pathogenicity of E. coli causing diarrhea in calves.DOI: http://dx.doi.org/10.3329/bjvm.v13i1.23716Bangl. J. Vet. Med. (2015). 13 (1): 45-51

scholarly journals Recent Antibiotic Sensitivity Pattern of Escherichia Coli in Urinary Tract Infection

2018 ◽  
Vol 30 (1) ◽  
pp. 61-65
Author(s):  
Md Abdul Bari ◽  
Mashiur Arefin ◽  
Moriom Nessa ◽  
Golam Mostofa ◽  
Tafiqul Islam
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Urine Culture ◽  
Antibiotic Sensitivity ◽  
E Coli ◽  
Sensitivity Pattern ◽  
Marked Resistance ◽  
Culture Positive

The objective of the study is to assess the recent sensitivity pattern of Escherichia coli in Urinary tract infection (UTI). Widespread use of antibiotics has led to the emergence of resistant microorganisms. As the antibiotic sensitivity patterns of the microorganisms are frequently changing, this retrospective analysis was designed to assess the recent antibiotic sensitivity pattern of Escherichia coli (E. coli) in urinary tract infection among the human population. Details of 358 urine culture positive reports for E. coli and their antibiotic sensitivity pattern pertaining to the study period of 6 months from Jan 2017 to June 2017 were collected from Microbiology Laboratory of Diagnostic Centers at Rajshahi and the results were statistically analyzed. The antibiotics tested for sensitivity were amikacin, gentamycin, ciprofloxacin, levofloxacin, cotrimoxazole, nitrofurantoin, ceftazidime, ceftriaxone and cefixime. The sensitivity pattern of E.coli to antibiotics in UTI was amikacin (82.68 gentamycin (75.97%), nitrofurantoin (70.67), levofloxacin (44.13%), ceftazidime (42.17%), co-trimoxazole (40.78%), ceftriaxone (38.54%), ciprofloxacin (35.75%), and cefixime (24.02%). The study highlighted the marked resistance of E. coli to quinolones and third generation cephalosporins.TAJ 2017; 30(1): 61-65

scholarly journals Citric acid: A potential permeabilizer against multiple drug resistance enteropathogenic Escherichia coli

2012 ◽  
Vol 4 (1) ◽  
pp. 87-91 ◽  
Author(s):  
Preeti G. Dharmik ◽  
Ashok V. Gomashe ◽  
Bharat J. Wadher
Keyword(s):  
Escherichia Coli ◽  
Citric Acid ◽  
Antimicrobial Agents ◽  
Multiple Drug Resistance ◽  
Antibiotic Sensitivity ◽  
Multiple Drug ◽  
Enteric Infection ◽  
E Coli ◽  
Enteric Diseases ◽  
Sensitivity Pattern

Enteric diseases enter through the mouth and are usually spread by contaminated food, water or contact with contaminated vomit or feces. Enteric infection encompasses all the infections of the intestinal tract. These intestinal infections include organisms like Escherichia coli, Salmonella, Shigella, Klebsiella, Proteus etc. Out of these, E. coli are one of the common causes of enteric infection. In spite the introduction of a wide variety of antimicrobial agents against enteric diseases, life threatening infections caused by E. coli contributes to morbidity and mortality in patients. The present study was conducted to determine the antibiotic sensitivity pattern of E. coli obtained from stool samples and potentiation of antibiotic activity by citric acid against multiple drug resistant E. coli. Out of the 200 isolates of E. coli, 150 were found to be resistant to one or more antibiotics tested. 0.05% and 0.1% citric acid was found to be effective in increasing the potency of the all the antibiotics used in the study.

scholarly journals The occurrence of disinfectant and antibiotic-resistant genes in Escherichia coli isolated from chickens in Egypt

2019 ◽  
Vol 12 (1) ◽  
pp. 141-145 ◽  
Author(s):  
Waleed A. Ibrahim ◽  
Sherif A. Marouf ◽  
Ahmed M. Erfan ◽  
Soad A. Nasef ◽  
Jakeen K. El Jakee
Keyword(s):  
Escherichia Coli ◽  
Resistance Genes ◽  
Diffusion Method ◽  
Reference Laboratory ◽  
Poultry Production ◽  
Isolation And Identification ◽  
Antibiotic Resistant ◽  
E Coli ◽  
Resistant Genes ◽  
Antibiotic Susceptibility Test

Aim: This work aimed to determine the occurrence of antibiotic and disinfectant resistance genes in Escherichia coli isolated from chickens in Egypt. Materials and Methods: Organs (liver, lung, heart, yolk sac, and bone marrow) of 1500 chicken samples were collected from diseased chickens suffered from colibacillosis with PM findings as CRD, diarrhea and omphalitis from different governorates of Egypt as: Giza, EL-Bahira, Fayoum, El-Dakahlia, El-Ismalia, and El-Sharkia during 2015-2016. These samples were labeled and transported immediately on ice to the Reference laboratory for quality control on poultry production (RLQP). The samples were cultured onto MacConkey agar and Eosin Methylene Blue Agar. Isolation and identification of the E. coli were performed based on morphology, cultural, staining, and biochemical properties. Antimicrobial resistance test was carried out using disk diffusion method. The PCR employing tetA, qacED1 and qacA/B were carried out for detection of these genes in isolated E.coli. Results: The prevalence of E. coli in chicken was 34%. Predominant serotypes of E. coli which serologically identified were O128, O111, O44, O158, and O2. Antibiotic susceptibility test of E. coli revealed that 100% of isolates were resistant to ampicillin, erythromycin, and sulfamethoxazole-trimethoprim, while 73.53% and 38.23% of them were sensitive for colistin sulfate and levofloxacin, respectively. Antibiotic resistance genes as tetA gene were tested for isolated E. coli and detected by incidence rate of 91.18%. qac resistance genes resembling as qacED1 and qacA/B genes were detected in isolated E. coli 70.6% and 14.7%, respectively. Conclusion: E. coli isolated from chickens in Egypt was carried qac and antibiotic-resistant genes that affect the poultry industry.

scholarly journals Screening for microbial load and antibiotic resistance pattern in Escherichia coli isolated from paper currency circulating in Kushtia, Bangladesh

2019 ◽  
Vol 7 (4) ◽  
pp. 1161 ◽  
Author(s):  
S. M. Raihan Rahman ◽  
Md. Nazim Uddin ◽  
Zulkar Nain ◽  
Mohammad Minnatul Karim
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Pathogenic Bacteria ◽  
Grocery Store ◽  
Human Beings ◽  
Isolation And Identification ◽  
Antibiotic Resistant ◽  
E Coli ◽  
Pseudomonas Spp ◽  
Paper Currency

Background: Paper currency is used for every type of commerce and plays an important role in the life of human beings. They are exchanged and come into contact with different environments and many different individuals during their circulation. Therefore, they can become contaminated with microorganisms and transfer bacteria across environments. The present study was aimed for quantitative assessment of microorganisms in circulated paper currency from Kushtia, Bangladesh and antibiotic resistant profiles of isolated Escherichia coli.Methods: A total of 10 paper currency samples currently in circulation involving three denominations (5, 10 and 500) were randomly collected from individuals involved in various occupations including street beggar, local hotel, bus conductor, poultry seller, vegetable seller, fish seller, commercial bank, ATM booth, tea seller, grocery store in Kushtia city, Bangladesh. Selective culture and biochemical tests were performed for the isolation and identification of microbial pathogens. Antibiotic resistance profiles were evaluated for isolated Escherichia coli using Kirby-Bauer method according to CLSI guidelines.Results: Aerobic mesophilic bacteria, Enterobacteriaceae and Pseudomonas spp. were the highest in paper currency from local hotel and ATM booth. Enterobacteriaceae (including coliforms) were predominantly present in paper currencies collected from local hotel, grocery, fish seller and beggar while Pseudomonas spp. were found in currency notes obtained from ATM booth, poultry farm, vegetable seller and local hotel. Antibiotic resistant profiles of E. coli isolated from local hotel currency showed that 50% of E. coli isolates were multidrug resistant. The highest resistant profile was observed against penicillin (95%) followed by polypeptide (75%), cephalosporin (50%), quinolone (30%) and sulfonamide (5%) groups of antibiotics.Conclusions: Multiple antibiotic resistant pathogenic bacteria are prevalent in paper currency regardless of their sources. Paper currency could contribute in transmission of infectious disease as well as in antibiotic resistance, therefore, should be handled carefully.

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