scholarly journals Standardization of age and route for duck plague vaccine in local ducklings of vaccinated and non- vaccinated parent origin

1970 ◽  
Vol 6 (1) ◽  
pp. 27-30
Author(s):  
MEH Kayesh ◽  
MSR Khan ◽  
MA Islam ◽  
MO Gani ◽  
MR Islam ◽  
...  
Keyword(s):  
Dose Rate ◽  
Subcutaneous Route ◽  
Passive Hemagglutination ◽  
Unvaccinated Control ◽  
Group A ◽  
Group B ◽  
Protection And Control ◽  
Key Words Vaccination ◽  
And Control ◽  
Plague Vaccine

The study was carried out to determine the appropriate age and route of vaccination with duck plague vaccine (LRI, Mohakhali) in experimentally reared local ducks during the period from September 2006 to May 2007. A total of 90 local ducklings were divided into eight groups namely A, C, D and E for vaccinated parent origin and B, F, G and H for non-vaccinated parent origin. Again Group C, D, E, F, G and H were divided into subgroups C1 & C2; D­1& D­2; E1& E2; F1 & F2; G1 & G2; H1 & H2 respectively and contained 5 ducklings each. Group A and B contained 15 ducklings in each group and were used as unvaccinated control and also for studying persistence of maternally derived antibody level. Highest mean MDA titre in ducklings of Group A was found 53.33 ± 4.03 at the age of day 1 that declined to a negligible level (≤4) at the age of day 21 and highest mean MDA titre in ducklings of Group B was found 29.86 ± 1.45 at the age of day 1 that declined to a negligible level (≤4) at the age of day 16. Ducklings of different sub-groups were vaccinated with duck plague vaccine at day 14, day 21 and day 28 through intramuscular (breast) and subcutaneous route at the dose rate of 1ml per duckling. Sera were collected from vaccinated ducklings on day 7, day 14 and day 21 after vaccination and antibody titre was measured by passive hemagglutination test. Among the vaccinated subgroups, the highest mean PHA titre was found 89.60 ± 15.67 and 83.20 ± 19.20 in subgroups E1 (vaccinated at day 28) and F1 (vaccinated at day 14) respectively. At challenge, ducklings of vaccinated subgroups showed 100% protection except C1 and C2 (showed 80% protection) and control subgroups showed 0% protection. From the results of protection test it may be concluded that both intramuscular (breast) and subcutaneous routes are equally suitable for duck plague virus vaccination in ducklings and the optimum age for vaccination to ducklings originated from vaccinated parent to duck plague vaccine might be at day 21 or 28 and ducklings originated from non-vaccinated parent at day 14 or 21 instead of usual schedule of day 28. Key words: Vaccination, duck plague vaccine, age, route, ducklings DOI = 10.3329/bjvm.v6i1.1335 Bangl. J. Vet. Med. 2008. 6 (1): 27-30

scholarly journals Comparative efficacy of BAU-fowl cholera and DLS-fowl cholera vaccines in indigenous chicken

2018 ◽  
Vol 5 (2) ◽  
pp. 193-199
Author(s):  
Chamak Nahar Shampa ◽  
Suma Akter ◽  
Sukumar Saha ◽  
Md Hadiuzzaman ◽  
Azhar Ul Alam ◽  
...  
Keyword(s):  
Pasteurella Multocida ◽  
Research Work ◽  
Booster Vaccination ◽  
Indigenous Chicken ◽  
Passive Hemagglutination ◽  
Fowl Cholera ◽  
Unvaccinated Control ◽  
Group A ◽  
The Mean ◽  
Group B

The present study was conducted to determine the immune response induced in indigenous chicken produced against BAU-FC and DLS-FC vaccines with their efficacy study against Pasteurella multocida. A total of forty (40) chickens were selected and divided into Group A (15), Group B (15) and Group C (10). Group A and B were vaccinated with BAU-FCV and DLS-FCV, respectively at the dose rate of 0.5 ml through SC at six weeks of age followed by boostering at 10 weeks of age while Group C was kept as unvaccinated control. Sera samples were collected after primary and booster vaccination and antibody titre was determined by Passive hemagglutination (PHA) test. The mean PHA titres recorded at 4 weeks after primary vaccination was 51.20 ± 7.84 in birds of group A and 38.40 ± 6.40 in birds of Group B. After booster vaccination, mean PHA titer was found 140.80 ± 31.35 at 16 weeks of age in case of BAU-FC vaccinated group and 115.20 ± 12.80 in case of DLS-FC vaccinated group. The mean PHA titer was 204.80 ± 31.35 and 179.20 ± 31.35 at 19 weeks of age in birds of BAU-FC and DLS-FC vaccinated group, respectively. Birds of all groups were challenged with virulent P. multocida at 17 weeks of age. It was observed that vaccinated chickens showed maximal resistance (100%) following challenge with virulent whereas unvaccinated control birds failed to resist the challenge infection. It can be assumed from the findings of present research work that both BAU-FCV and DLS-FCV are able to protect indigenous chicken from the outbreak of avian pasteurellosis and BAU-FV vaccine showed relatively higher immuno-protective titre than that of DLS-FC vaccine.Res. Agric., Livest. Fish.5(2): 193-199, August 2018

scholarly journals Effects of triclabendazole and nitroxynil on EPG, hematological parameters and body weight against fascioliasis in goats at government goat development farm, Sylhet, Bangladesh

2016 ◽  
Vol 5 (2) ◽  
pp. 46-51 ◽  
Author(s):  
S Khanam ◽  
MS Islam ◽  
M Aktaruzzaman ◽  
MA Hossain ◽  
MM Hossain ◽  
...  
Keyword(s):  
Body Weight ◽  
Dose Rate ◽  
Hematological Parameters ◽  
Experimental Period ◽  
The Body ◽  
Control Group ◽  
Infected Control Group ◽  
Group A ◽  
Group B ◽  
And Control

The study was conducted to evaluate the effects of triclabendazole and nitroxynil against fascioliasis in goat for a period of 28 days on 20 black bengal goats. Among hundred goats, twenty were selected for this study which was suffering from liver fluke (Fasciola gigantica) infection and were marked by tagging. Twenty goats were randomly divided into four groups (A, B, C and D) where each group comprising of five goats. Group A were treated with tablet fasinex (triclabendazole, Novartis Ltd, Bangladesh) orally at the dose rate of 12 mgkg-1body weight, group B were treated with acinex (triclabendazole, ACI Ltd, Bangladesh) orally at the dose rate of 12 mgkg-1body weight and group C were treated with nitronex injection (nitroxynil, Renata Ltd, Bangladesh) subcutaneously at the dose rate of 10 mgkg-1 body weight. Goats of group D was kept as infected control group without giving any treatment. A significant (P<0.01) reduction of egg per gram (EPG) count was found on 7th, 14th, 21st and 28th days of treated goats of group A, B and C, respectively. The EPG count of control group were significantly (P<0.01) increased 7 day onwards up to experimental period. Reduction of mean EPG on 7th, 14th, 21st and 28th day after treatment in group A, B and C were 81.48%, 81.25% and 83.04%, respectively, whereas in control group the mean EPG were 22.43%. Of the hematological parameters, TEC, Hb and PCV values were lower on day 0 but turned to increase (p<0.01) on day 28 of the study. On the other hand, ESR and TLC were higher before treatment (day 0) but decreased significantly (p<0.01) on day 28.The body weight increased significantly (P<0.01) after treatments in group A, B and C respectively except untreated control. This result may indicate that three commercial anthelmintics contain triclabendazole and nitroxynil against fascioliasis in goat and nitroxynil have higher efficacies on egg per gram (EPG), hematological parameters and body weight against fascioliasis in goat. Additional detailed studies are required to prevent and control of fascioliasis in goats by using a routine prophylactic anthelmintics measurement.International Journal of Natural Sciences (2015), 5(2) 46-51

scholarly journals Comparative efficacy of imported fowl pox virus vaccine with locally produced one in backyard chicks

1970 ◽  
Vol 6 (1) ◽  
pp. 23-26 ◽  
Author(s):  
MR Islam ◽  
MSR Khan ◽  
MA Islam ◽  
MEH Kayesh ◽  
MR Karim ◽  
...  
Keyword(s):  
Virus Vaccine ◽  
Control Group ◽  
Post Vaccination ◽  
History Of ◽  
Group A ◽  
Group B ◽  
Protection And Control ◽  
And Control ◽  
Group D

A total of 80 experimentally reared backyard chicks with the history of non-vaccinated parents to FPV vaccine were used for the determination of persistence of maternally derived antibody in chicks and to compare the efficacy of Poxine® and DLS-FPV vaccine in protecting the chicks. Chicks were divided into five groups namely group A, B, C, D and E where each group contained 15 chicks except group A contained 20 chicks which was also used to determine the persistence of maternally derived antibody (MDA). Birds of group B and C were vaccinated with DLS-FPV at day 22 and 18 respectively through wing web puncture (WWP), whereas group D and E were vaccinated with Poxine® at day 36 and 18 respectively. Sera were randomly colleted from 10 chicks of each group at 7, 14 and 21 days of post vaccination for the determination of antibody titre using PHA test. Ten chickens from each group were challenged three weeks post vaccination with 106 EID50/0.1ml. "Take reaction" was recorded to assess the better immune response in different groups after vaccination. Birds of group B, C, D and E showed 100, 86.67, 93 and 86.67% "take reaction", respectively. The highest Mean PHA titre was found 217.60 ± 19.55 in the chicks of group B. After challenge, the birds of group B and D showed 100% protection, whereas birds of group C and E showed 93.33% protection and control group showed no protection. From the results of PHA test it may be concluded that both Poxine® and DLS-FPV vaccines are equally suitable and the chicks of nonvaccinated origin might be vaccinated with DLS-FPV at day 18 or 22 and 36 in case of Poxine® fowl pox virus vaccine. Key words: Efficacy, fowl pox virus vaccine, backyard chicks, age DOI = 10.3329/bjvm.v6i1.1334 Bangl. J. Vet. Med. (2008). 6 (1): 23-26

Resorbable Collagen Barrier Impeding the Extrusion of Obturating Material in Primary Molars Undergoing Resorption – A Randomized Clinical Trial

2021 ◽  
Vol 45 (5) ◽  
pp. 312-316
Author(s):  
Mishra Neha Sanjeev ◽  
Harsimran Kaur ◽  
Sandeep Singh Mayall ◽  
Rishika ◽  
Ramakrishna Yeluri
Keyword(s):  
Test Group ◽  
Primary Molars ◽  
Control Group ◽  
Chi Square ◽  
Significance Level ◽  
Significant Difference ◽  
Chi Square Test ◽  
Group A ◽  
Group B ◽  
And Control

Objective: To evaluate the effectiveness of placing a resorbable collagen barrier in impeding the extrusion of obturation material in primary molars undergoing resorption. Study design: All the 94 canals in 47 mandibular molars were allocated to 2 groups- Group ‘A’- 47 canals with collagen barrier (Test group) and Group ‘B’- 47 canals without collagen barrier (Control group) based on randomization protocol. Pulpectomy was performed and obturation of both test and control canals were radiographically assessed. Pearson’s chi – square test was applied to analyze the results. The significance level was predetermined at p &lt; 0.05. Results: Among the test group, 93.6% of the canals showed no extrusion while, 6.4% showed visible extrusion of the material outside the apex. In the control group, 83% showed no extrusion whereas 17% of the canals showed visible extrusion outside the apex. But no significant difference was noted (p&gt;0.05). Conclusion: The placement of resorbable collagen barrier in the apical third of the canal prevented the extrusion of obturating material beyond the apex in resorbing primary molars.

Electrical Stimulation of Intact or Split Steer or Cow Carcasses

1980 ◽  
Vol 43 (10) ◽  
pp. 795-798 ◽  
Author(s):  
F. K. McKEITH ◽  
C. G. SMITH ◽  
T. R. DUTSON ◽  
J. W. SAVELL ◽  
R. L. HOSTETLER ◽  
...  
Keyword(s):  
Electrical Stimulation ◽  
Structural Damage ◽  
Troponin T ◽  
Protein Subunits ◽  
Versus Group ◽  
Group A ◽  
Group B ◽  
The Right ◽  
And Control ◽  
Group D

Fifteen carcasses, 10 from steers and 5 from cows, were used for the present study. Five steer carcasses (group A) were electrically stimulated as intact, unsplit carcasses. The left sides of 5 steer carcasses (group B) and of 5 cow carcasses (group C) were electrically stimulated; the right sides of the same 5 steer carcasses (group D) and of the same 5 cow carcasses (group E) were used as controls and were not electrically stimulated. Electrically stimulated carcasses and sides (groups A and B) had brighter, more youthful colored lean, less “heat-ring” and produced more tender and more palatable rib steaks than did control sides (group D). Electrical stimulation did not (P &gt; .05) affect ultimate pH or sarcomere length in steers or cows. Light and electron micrographs revealed increased (P &lt; .05) structural damage (more severe contracture bands) in steer or cow muscles from electrically stimulated sides than in muscles from control sides; however, structural damage was not (P &gt;.05) increased when intact steer carcasses were electrically stimulated and compared to unstimulated sides. Troponin-T was reduced in SDS gels of muscle from electrically stimulated, as compared to control, sides of cow carcasses (group C versus group E); no differences in percentage of protein subunits were observed between electrically stimulated and control sides of steer carcasses (group B versus group D). Electrical stimulation can be done on intact carcasses or sides of young beef to improve USDA lean maturity and lean color scores, to reduce “heat-ring” incidence and to improve tenderness.

scholarly journals The Efficacy of Ranikhet Disease Vaccines Produced by Livestock Research Institute of Bangladesh

2013 ◽  
Vol 1 (1) ◽  
pp. 9-13 ◽  
Author(s):  
Sreebas Chandra Sarkar ◽  
Sukumar Saha ◽  
Md Mansurul Amin ◽  
Md Golzar Hossain
Keyword(s):  
Field Isolate ◽  
Challenge Infection ◽  
Control Group ◽  
Passive Protection ◽  
Field Virus ◽  
Unvaccinated Control ◽  
Antibody Titers ◽  
Group A ◽  
Group B ◽  
Research Institute

The study was conducted to investigate the efficacy of Baby chick Ranikhet Disease Vaccine (BCRDV) and Ranikhet Disease Vaccine (RDV) produced by the Livestock Research Institute (LRI), Mohakhali, Dhaka. For this experiment, 100 day-old-chick was purchased from Phinex Hatchery Ltd., Gazipur. The chicks (n=100) were divided into two groups. In group A (n=50), vaccination was performed twice with BCRDV at 2 and 21 days of age through intraocular route (i/o) followed by once with RDV at 60-day of age through intramuscular (i/m) route. Group B (n=50) was kept as unvaccinated control. The immunogenicity of the vaccine was evaluated by measuring the serum HI antibody titers at 1-, 20-, 36-, and 76-day of age, while the vaccine efficacy was examined by a challenge infection experiment with a velogenic field isolate of NDV as well as passive protection test. It was observed that the maternal antibody titers of the unvaccinated control group B gradually declined from day 1 to day 76 of age. Conversely, after primary and secondary vaccination with BCRDV, the levels of serum HI titer slightly increased in vaccinated group A compared with those in control group B. Finally administration of RDV resulted in a sharp increase in HI titer, leading to protection from challenge infection with virulent field virus as well as passive protection test. These results clearly demonstrated that a prime-booster immunization with BCRDV and RDV, both produced by LRI, is effective to protect chicken against Newcastle disease (ND).DOI: http://dx.doi.org/10.3329/mh.v1i1.13706 Microbes and Health Vol.1(1) June 2012 pp.9-13

scholarly journals P0645EDARAVONE MAY DECREASE THE INCIDENCE OF CONTRAST INDUCED ACUTE KIDNEY INJURY (CI-AKI)

2020 ◽  
Vol 35 (Supplement_3) ◽  
Author(s):  
Dmytro Ivanov ◽  
Mariia Ivanova ◽  
Illia Burlachenko
Keyword(s):  
Contrast Media ◽  
Kidney Injury ◽  
Peroxyl Radicals ◽  
Controlled Study ◽  
Control Group ◽  
Ckd Stage ◽  
Intravenous Hydration ◽  
Group A ◽  
Group B ◽  
And Control

Abstract Background and Aims Edaravone is a low-molecular-weight antioxidant drug targeting peroxyl radicals of reactive oxygen species and has shown clinical efficacy in patients with acute ischemic stroke. We have conducted a multicenter open prospective randomized controlled study to evaluate the efficacy of edaravone in preventing AKI in patients with CKD 3b-4 stages. Method The study included 2 groups of patients aged 46 to 68 (55 ± 3): group A (n=16) with CKD stage 3b or 4 (eGFR EPI 32 ± 4 ml/min) that received intravenous edaravone 30 mg bid on 0,1,2 day of contrast media infusion and control group B (n=20) with CKD stage 3b or 4 (eGFR EPI 33 ± 3 ml/min) with no edaravone intervention during CT coronarography. Patients of both groups received intravenous hydration with 0.9% sodium before CT. Primary endpoint: CIN onset in 48 hours after contrast media infusion and need for RRT. Secondary endpoint: serum potassium level above 5.5 mmol/l. Results CIN onset was obtained in 4 patients of group A and 12 patients of group B (p&gt;0,05, RR 0.417, RRR 0.583, RD 0.350, NNT 2.857). Other results are presented in table

scholarly journals A Novel Method to Enhance Dynamic Rhinoplasty Outcomes: Double “V” Carving for Alloplastic Grafts

2019 ◽  
Vol 99 (4) ◽  
pp. 262-267 ◽  
Author(s):  
Shan-shan Bai ◽  
Dong Li ◽  
Liang Xu ◽  
Hui-chuan Duan ◽  
Jie Yuan ◽  
...  
Keyword(s):  
Practical Method ◽  
Control Group ◽  
Augmentation Rhinoplasty ◽  
3 Dimensional ◽  
Asian Patients ◽  
Subjective Evaluations ◽  
Group A ◽  
Novel Method ◽  
Group B ◽  
And Control

Augmentation rhinoplasty is one of the most common plastic surgery procedures performed in Asia. Most Asian patients desire not only a natural-looking nose but also a nose with natural feel. Achieving such rhinoplasty outcomes with grafts has been a challenge for surgeons due to rigidity of grafting material. We propose a novel technique to address this limitation. A total of 200 healthy adult patients aged from 18 to 25 years were randomly chosen and classified into 5 groups: A, B, C, D, and control. Each group included 40 patients. The patients assigned to conventional grafting underwent rhinoplasty with L-shaped silicone prosthesis (group A) or expanded polytetrafluoroethylene (e-PTFE; group B), using traditional carving methods. The patients assigned to dynamic rhinoplasty underwent silicone (group C) or e-PTFE grafts (group D) using the modified double “V” method, which involves removing bilateral wedges from the graft to decrease rigidity. Patients in control group do not undergo the surgery. A 3-dimensional raster surface scanner was used to capture the images of the patients accurately and nasal mobility was measured. Subjective evaluations were carried out by a series of questionnaires asked to the patients. The angle α of nasal mobility was significantly lower in conventional grafting (23.09 [5.34] mm for silicone and 17.88 [4.96] mm for e-PTFE) versus the “V” carving (30.53 [3.76] mm for silicone and 23.77 [4.53] mm for e-PTFE; P < .05). The double “V” carving method is a simple, effective, and practical method for improving dynamic nasal outcomes in patient undergoing augmentation rhinoplasty.

scholarly journals HUMAN IMMUNITY TO THE MENINGOCOCCUS

1969 ◽  
Vol 129 (6) ◽  
pp. 1349-1365 ◽  
Author(s):  
Emil C. Gotschlich ◽  
Teh Yung Liu ◽  
Malcolm S. Artenstein
Keyword(s):  
Molecular Weight ◽  
Hemagglutination Inhibition ◽  
Neuraminic Acid ◽  
Purification Procedure ◽  
Main Constituent ◽  
Passive Hemagglutination ◽  
Hemagglutination Inhibition Test ◽  
Group A ◽  
Human Immunity ◽  
Group B

The group-specific polysaccharides of group A and group C meningococci have been isolated by a new procedure which employs the cationic detergent Cetavlon to precipitate these polysaccharides from the whole culture. The A and C polysaccharide prepared by this method are noteworthy because they are of high molecular weight. The main constituent of the A polysaccharide is N-acetyl, O-acetyl mannosamine phosphate; of the C polysaccharide N-acetyl, O-acetyl neuraminic acid. This purification procedure, when applied to cultures of group B organisms, yields a polysaccharide consisting primarily of N-acetyl neuraminic acid. A passive hemagglutination test developed to measure antibodies to the polysaccharides demonstrated the specificity of these antigens. Using a hemagglutination inhibition test, these antigens were again found to be group-specific, and this test could be used for serogrouping meningococcal isolates.

Effects of exogenous growth hormone pretreatment on the pituitary growth hormone response to growth hormone-releasing hormone alone or in combination with pyridostigmine in Type I diabetic patients

1991 ◽  
Vol 125 (5) ◽  
pp. 510-517 ◽  
Author(s):  
Andrea Giustina ◽  
Simonetta Bossoni ◽  
Corrado Bodini ◽  
Antonino Cimino ◽  
Giuseppe Pizzocolo ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Blood Glucose ◽  
Normal Subjects ◽  
Diabetic Patients ◽  
Type I ◽  
Gh Secretion ◽  
Glucose Levels ◽  
Group A ◽  
Group B ◽  
And Control

Abstract. We evaluated the effects of iv pretreatment with exogenous GH on the GH response to GHRH either alone or in combination with pyridostigmine in 14 Type I diabetic patients and 6 normal subjects. All the subjects received an iv bolus injection of biosynthetic human GH, 2 IU; 2 h later they received either a. pyridostigmine, 120 mg orally, or b. placebo, 2 tablets orally, followed 1 h later by iv injection of GHRH(1-29) NH2, 100 μg. In normal subjects the median GH peak after GH+GHRH was 1.8, range 1.2-6.9 μg/l. Pyridostigmine enhanced the GH response to GHRH in all subjects. The median GH peak after pyridostigmine+ GH+GHRH was 32.7, range 19.8-42.1 μg/l (p<0.001 vs GHRH alone). Seven diabetic subjects had median GH peaks after GH+GHRH >6.9 μg/l (the maximum GH peak after GH+GHRH in normal subjects) (group A: median GH peak 35.7, range 21.7-55 μg/l). The other diabetic subjects had GH peak lower than 6.9 μg/l (group B: median GH peak 4.4, range 2.1-6.5 μg/l). Pyridostigmine significantly increased the GH response to GHRH in group B patients (median GH peak 29.3, range 15.7-93.4 μg/l, p<0.001 vs GH+GHRH alone), but not in group A patients (median GH peak 39.9, range 21.9-64.9 μg/l). Group A diabetic patients were younger and had higher HbA1c and blood glucose levels than group B patients. In those diabetic patients with an exaggerated GH response to GH+GHRH, pyridostigmine failed to cause the increase in GH secretion observed in diabetic and control subjects with no responses to GH+GHRH. It can be suggested that elevated 24-h GH levels in some Type I diabetic patients may be due to decreased somatostatinergic tone which in turn causes altered autoregulation of GH secretion. We hypothesize that this finding is a consequence of a reset of the hypothalamic control of GH secretion caused by a chronically elevated blood glucose level in this subpopulation.

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