scholarly journals Factors influencing the pregnancy rate in indigenous ewes following AI using frozen semen

2018 ◽  
Vol 33 (2) ◽  
pp. 33-38 ◽  
Author(s):  
N Naher ◽  
NS Juyena ◽  
PK Jha ◽  
MRI Talukder ◽  
MGS Alam ◽  
...  
Keyword(s):  
New Zealand ◽  
Treatment Group ◽  
Pregnancy Rate ◽  
Penetration Depth ◽  
Artificial Insemination ◽  
Prostaglandin F2α ◽  
Factors Influencing ◽  
Medium Intensity ◽  
Heat Detector ◽  
Frozen Semen

Study was conducted to observe the influence of different factors on pregnancy rate in 24 indigenous ewes following artificial insemination (AI) with frozen semen. The ewes were synchronized by intra-muscular injection of Prostaglandin F2α (Ovuprost® Bayer, New Zealand). The onset and intensity of oestrus were determined by oestrus behaviour of ewes with vasectomized ram. VER values were measured immediately before AI using electrical heat detector (DRAMINSKI®, Owocowa 17, Poland). Twelve ewes were inseminated trans-cervically and 12 laparoscopically. Five ewes were treated with Misoprostol (Cytomis® 200 µg tablet; Incepta Pharmaceuticals Ltd. Bangladesh) to relax the cervix 12 hours before TCAI, and cervical penetration depth was measured by a steel rod. Pregnancy was confirmed by ultrasonography at 40 – 45 days after AI. Cervical penetration depth was significantly increased (P<0.05) (0.48 ± 0.04 vs. 3.52 ± 0.17 cm) in treatment group. The pregnancy rate tended to be higher in treatment group (60.0 vs. 28.6%). In treatment group cervical penetration was significantly increased (P<0.05) (0.35 ± 0.08 vs. 3.52 ± 0.17 cm) immediately before AI compared with before oestrus synchronization. The pregnancy rate was significantly higher (P<0.05) (75.0 vs. 28.6%) in LAPAI than TCAI without treatment. The pregnancy rate tended to be higher (75.0 vs. 55.6%) in ewes with high oestrus intensity than in those with medium intensity. The pregnancy rate was significantly higher (P<0.05 : 70.0 vs. 20.0%) in lower VER group (230 - 280 Ω) compared with higher VER group (331 – 380 Ω.) Although LAPAI was superior to TCAI, TCAI in ewes treated with misoprostol giving a pregnancy rate of 60% could be acceptable, until the LAPAI could be made simpler to be used in field. The oestrus intensity and low VER values could be used to select ewes to be inseminated to increase the pregnancy rate.Bangl. vet. 2016. Vol. 33, No. 2, 33-38

scholarly journals The Effect of A PRID®Delta-Based Estrus Synchronization Protocol On Pregnancy Rates In Cows With No Estrus Symptoms

2022 ◽  
Author(s):  
Murat Can Demir ◽  
Cihan Kaçar ◽  
Umut Çağın Arı ◽  
Semra Kaya ◽  
Oğuz Merhan ◽  
...  
Keyword(s):  
Pregnancy Rate ◽  
Artificial Insemination ◽  
Prostaglandin F2α ◽  
Pregnancy Rates ◽  
Dominant Follicle ◽  
Serum Progesterone ◽  
Blood Samples ◽  
Follicle Diameter ◽  
Follicle Size ◽  
Equine Chorionic Gonadotropin

Abstract The present study aimed to determine the effects of progesterone-based gonadotropin-releasing hormone (GnRH), prostaglandin F2α (PGF2α), and equine chorionic gonadotropin (eCG) injections on progesterone profiles and pregnancy rates in cows with no estrus symptoms within 60 days after parturition. A total of 80 cows were included in the study. All animals had the progesterone-releasing device PRID®Delta placed intravaginally for nine days with an injection of GnRH. On the eighth day, PGF2α was injected, and PRID®Delta was removed from the vagina on day nine. Artificial insemination was carried out 60 hours after PRID®Delta removal. In half of the animals (n = 40), 600 IU of eCG was injected when PRID®Delta was removed on the ninth day before artificial insemination 60 hours later. Blood samples were taken from the tail vein on days 0 and 8 to determine progesterone levels. The pregnancy rate in the group that received eCG was 37.5%, while it was 27.5% in those that did not (P = 0.4). While the dominant follicle diameter was 15.5 mm in cows injected with eCG during timed artificial insemination, the follicle diameter was 12.4 mm in cows with no eCG injection (P <0.001). There were no differences in serum progesterone values in blood samples taken until the time of artificial insemination. However, progesterone values in the blood taken during artificial insemination were 0.94 ng/ml in the eCG- group and 0.72 ng/ml in the eCG+ group (P <0.05). As a result, it was determined that eCG injections, in addition to progesterone-based GnRH and PGF2α applications, increased the pregnancy rates in cows without symptoms of estrus. The dominant follicle diameter was larger in cows treated with eCG during artificial insemination; however, follicle size did not increase the pregnancy rate.

scholarly journals Effect of green tea extract in extender of Simmental bull semen on pregnancy rate of recipients

2021 ◽  
Vol 34 (2) ◽  
pp. 198-204 ◽  
Author(s):  
Suherni Susilowati ◽  
Trilas Sardjito ◽  
Imam Mustofa ◽  
Oky Setio Widodo ◽  
Rochmah Kurnijasanti
Keyword(s):  
Pregnancy Rate ◽  
Dna Fragmentation ◽  
Green Tea ◽  
Prostaglandin F2α ◽  
Green Tea Extract ◽  
Control Group ◽  
Bull Semen ◽  
Frozen Semen ◽  
Tea Extract

Objective: The aim of this study was to ascertain the effects of adding green tea extract (GTE) to skim milk-egg yolk (SM-EY) extender on both the quality of post-thawed bull semen and the pregnancy rates of the recipient cows.Methods: Twelve ejaculates from four Simmental bulls, aged 3 to 5 years and weighing 900 to 950 kg, were diluted SM-EY extender, added with 0, 0.05, 0.1, and 0.15 mg GTE/100 mL extender and then frozen. After four weeks storage in liquid nitrogen, the sperm were thawed and evaluated for viability, motility, intact plasma membrane (IPM), and DNA fragmentation. Meanwhile, the estrus cycles of 48 recipient cows were synchronized by intramuscular administration of a single injection of 5 mg prostaglandin F2α. Estrus cows were divided into four equal groups and inseminated artificially 18 to 20 h after the onset of estrus by using semen from each extender group. Pregnancy was diagnosed by measuring serum progesterone levels at 21 days, followed by transrectal palpation 90 days after insemination.Results: The findings revealed that adding 0.1 mg of GTE/100 mL extender produced the highest percentages of sperm viability (70.67%±1.75%), motility (69.17%±1.47%), and IPM (69.23%±1.21%) and the lowest percentage of DNA fragmentation (3.00%±0.50%). The pregnancy diagnosis revealed that all cows (36/36) inseminated using frozen semen in GTE addition extender were pregnant (pregnancy rate 100%), whereas the pregnancy rate of the control group was 83.33% (10/12).Conclusion: It may be concluded that 0.1 mg GTE/100 mL extender yields the best quality of spermatozoa and that all variants doses of GTE in extender produce a higher pregnancy rate among recipient cows.

scholarly journals Estrus and pregnancy rate of Simmental-Ongole Crossbred and Ongole Grade heifer after being synchronized and inseminated

2018 ◽  
Vol 43 (4) ◽  
pp. 438
Author(s):  
S. Sutiyono ◽  
D. Samsudewa ◽  
A. Suryawijaya
Keyword(s):  
Pregnancy Rate ◽  
Artificial Insemination ◽  
Reproductive Organs ◽  
Estrus Synchronization ◽  
Purposive Sampling ◽  
Frozen Semen ◽  
Research Population ◽  
Rectal Palpation

The aim of the study was to examine the synchronization of estrus and artificial insemination in heifers of Ongole grade and Simental-Ongole grade. The research used 19 Ongole Grade (OG) and 18 Simental-Ongole Crossbred (SOC) heifers. This research applied purposive sampling to determine the research population. The criteria to select the population were heifers which have in mature body. The normality of heifer’s reproductive organs were identified by performing rectal palpation, then 50 mg of medroxy progesterone acetate on vaginal sponge was used to synchronized estrus. The estrus heifer was inseminated using frozen semen. The observed parameters were the number of heifers with normal and abnormal reproductive organs, estrus sign and pregnancy. Data were analyzed descriptively. This study showed that OG and SOC with abnormal reproductive organs was 1 heifer (5.56%) and 7 heifers (36.84%), respectively. The responses of estrus synchronization in heifers with normal and abnormal reproductive organs were 94.17% and 100% in OG, and 100% and 42.86% in SOC. Meanwhile, the pregnancy rate was 70.59 and 0% in OG, and 50.00 and 0% in SOC. In conclusion, the number of SOC heifers with abnormal reproductive organs is higher than OG. Estrus synchronization using 50 mg medroxy progesterone acetate increase the amount of estrus and pregnancy in heifers with normal reproductive organs.

The influence of purified porcine relaxin on the reproductive performance of sows following artificial insemination with fresh or frozen semen

1991 ◽  
Vol 71 (1) ◽  
pp. 237-239 ◽  
Author(s):  
P. A. Thacker ◽  
A. D. Gooneratne ◽  
R. N. Kirkwood
Keyword(s):  
Key Words ◽  
Pregnancy Rate ◽  
Litter Size ◽  
Reproductive Performance ◽  
Artificial Insemination ◽  
Pregnancy Rates ◽  
Frozen Semen

Pregnancy rates and litter sizes following insemination of sows with fresh-extended and frozen-thawed semen averaged 71 vs. 53% and 10.6 vs. 4.4, respectively, and was not affected by the addition of 12.5 μg relaxin to semen. These results indicate no relaxin mediated effect on either pregnancy rate or litter size when sows are artificially inseminated with relaxin-supplemented semen. Key words: Artificial insemination, relaxin, sows

19 FACTORS AFFECTING PREGNANCY RATE AFTER ARTIFICIAL INSEMINATION WITH EQUINE SEMEN: PRODUCTION OF THE FIRST FOAL USING FROZEN SEMEN IN KOREA

2011 ◽  
Vol 23 (1) ◽  
pp. 116
Author(s):  
Y. S. Park ◽  
Y. G. Cho ◽  
K. H. Cho ◽  
G. J. Cho
Keyword(s):  
Pregnancy Rate ◽  
Artificial Insemination ◽  
Technology Development ◽  
Skim Milk ◽  
Development Program ◽  
Field Trials ◽  
Pregnancy Rates ◽  
Chi Square ◽  
Semen Collection ◽  
Frozen Semen

Research in the area of equine artificial insemination (AI) has led to its increased application in field trials (Loomis and Graham, 2008). However, procedures for equine semen collection, cooling and freezing of semen and artificial insemination need further improvement. This study examined the effect of semen preparation and ovulation on equine AI success. Stallion semen was collected via artificial vagina, diluted with skim-milk extender, and preserved by either cooling to 4°C or cryopreservation and storage in liquid nitrogen. Mares were examined by ultrasound for follicle development with every 12 h during oestrus. During AI, semen was inserted into uterine horn adjacent to the ovulating follicle sized over 4 cm and irregular shape. In experiment 1, mares were inseminated with either cooled, cooled-transported, or frozen–thawed semen. Cooled semen was used immediately after collection and cooling. Cooled-transported semen was used 4 h after semen collection and dilution. Semen freezing procedure was followed by Moore et al. (2006). In experiment 2, we examined the effect of buffer (skim-milk extender), which was infused into the uterus at the time of AI with frozen–thawed semen. In experiment 3, we compared AI pregnancy rates for mares ovulating spontaneously v. after treatment with hCG. Pregnancy data were analysed Chi square test where P-values < 0.05 were considered significantly different. In experiment 1, the pregnancy rates after AI using cooled, cooled-transported, and frozen–thawed semen were 60 (10/6), 50 (8/4), and 37.5% (8/3), respectively, and similar among treatments. In experiment 2, the pregnancy rate of mares infused with buffer at AI was 37.5% (8/3) which was higher than that with no buffer (0%; 6/0). In experiment 3, the pregnancy rates of mares were similar between ovulated spontaneously (25%; 8/2) and ovulated with hCG (50%; 8/4). The results suggest that equine semen that has been cooled, cooled and transported or frozen can be successfully used to establish AI pregnancy. Also, the pregnancy rates after AI can be increased by infusing buffer into the uterus at AI or by inducing ovulation with hCG. These experiments resulted in the first foal produced in Korea with frozen–thawed equine semen. This research was supported by Technology Development Program for Agriculture and Forestry, Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea.

7 COMPARISON OF TIMED ARTIFICIAL INSEMINATION PROTOCOLS USING PROSTAGLANDIN F2α+ESTRADIOL OR PROSTAGLANDIN F2α+ESTRADIOL+GONADOTROPIN-RELEASING HORMONE IN DAIRY COWS

2012 ◽  
Vol 24 (1) ◽  
pp. 115
Author(s):  
J. K. Jeong ◽  
H. G. Kang ◽  
I. H. Kim
Keyword(s):  
Dairy Cows ◽  
Pregnancy Rate ◽  
Artificial Insemination ◽  
Prostaglandin F2α ◽  
Pregnancy Rates ◽  
Gnrh Analogue ◽  
Chi Square ◽  
Array Transducer ◽  
Chi Square Test ◽  
Timed Artificial Insemination

This study compared pregnancy rates following 2 timed artificial insemination (TAI) protocols using PGF2α + oestradiol or PGF2α + oestradiol + GnRH in dairy cows. Four hundred fifty-five Holstein dairy cows, 2.2 ± 0.1 in parity, 148.3 ± 3.6 days in milk, with corpus luteum of greater than 20 mm diameter confirmed by ultrasonography (Tringa Linear with 5.0 MHz array transducer; Esaote Pie Medical, Maastricht, the Netherlands) were randomly divided into two treatments: an injection of 500 μg of cloprostenol, PGF2α analogue (Iliren cycle BP®, Intervet International GmbH, Unterschleissheim, Germany) and an injection of 2 mg of oestradiol benzoate (EB, SY Esrone, Samyang, Seoul, Korea) 36 h later (PGF2α + EB group, n = 257), or an injection of 500 μg of cloprostenol, an injection of 2 mg of EB 36 h later and an additional injection of 100 μg of gonadorelin, GnRH analogue (Godorel, Uni-Biotech Co., Ltd., Korea) 24 h later (PGF2α + EB + GnRH group, n = 198). All cows in the two groups received TAI 24 h after the EB injection. Pregnancy was determined at 40 to 50 days after TAI by using both ultrasonography and rectal palpation. Pregnancy rates between the PGF2α + EB and PGF2α + EB + GnRH groups were compared by the chi-square test using the SAS program (version 9.1: SAS Institute Inc., Cary, NC, USA). Pregnancy rate following TAI did not differ between the PGF2α + EB (32.7%) and PGF2α + EB + GnRH groups (34.9%; P > 0.05). In conclusion, addition of GnRH for TAI protocol using PGF2α + oestradiol did not improve pregnancy rate in dairy cows.

scholarly journals Recombinant bovine somatotropin in the synchronization of ovulation in crossbred dairy cows (Bos taurus indicus × Bos taurus taurus)

2020 ◽  
Vol 13 (4) ◽  
pp. 746-750
Author(s):  
Fabrício Albani Oliveira ◽  
Ítalo Câmara de Almeida ◽  
Larissa Marchiori Sena ◽  
Jurandy Mauro Penitente-Filho ◽  
Ciro Alexandre Alves Torres
Keyword(s):  
Pregnancy Rate ◽  
Artificial Insemination ◽  
Bos Taurus ◽  
Prostaglandin F2α ◽  
Estradiol Benzoate ◽  
Bovine Somatotropin ◽  
Recombinant Bovine Somatotropin ◽  
Equine Chorionic Gonadotropin ◽  
The Moment ◽  
Follicular Dynamics

Aim: The aim of this study was to evaluate the effects of the administration of recombinant bovine somatotropin (rbST) at the moment of implementation of the timed artificial insemination protocol, on follicular dynamics and pregnancy rate in crossbred cows. Materials and Methods: A total of 346 cows were used in two experiments with a factorial 2×2 design. The cycling cows (Tcycling) and the anestrous cows (Tanestrous) were considered as factor 1 and the administration of rbST (TrbST) or not (Tcontrol) as factor 2. The experimental protocol: (1) Tcontrol – day 0 (D0), insertion of a progesterone-release intravaginal device (PRID) plus 2 mg of estradiol benzoate (EB); D8, PRID removal, plus 0.150 mg of prostaglandin F2α, and 400 IU of equine chorionic gonadotropin; D9, 1 mg of EB; and with artificial insemination at day 10; (2) TrbST – similar to Tcontrol plus 500 mg of rbST on D0. In experiment I, ultrasound examinations were performed in all treatments. In experiment II, the cows' pregnancy rate was evaluated. Data were analyzed with 5% probability. Results: There was no effect of the protocols on cows cyclicity or follicular growth rate (p>0.05). There was no interaction of the effects, administration of rbST, and the cyclicity of cows on the pregnancy rate. The total pregnancy rate observed was 49.0%. The pregnancy rate in cows receiving rbST was lower for anestrous compared with cycling cows (p<0.05). Conclusion: The administration of rbST did not alter the patterns of follicular dynamics nor the ovulation rate. However, cows in anestrous that received rbST had lower pregnancy rates than cycling cows.

160 PREGNANCY RATES AFTER USING THE CIDR-G DEVICE AND FIXED-TIMED ARTIFICIAL INSEMINATION IN WHITE-TAIL DEER

2008 ◽  
Vol 20 (1) ◽  
pp. 160
Author(s):  
G. T. Gentry Jr ◽  
J. Saenz ◽  
W. Forbes ◽  
K. Whitehead ◽  
D. Sanders ◽  
...  
Keyword(s):  
Body Weight ◽  
Treatment Group ◽  
Artificial Insemination ◽  
Prostaglandin F2α ◽  
Estradiol Benzoate ◽  
Pregnancy Rates ◽  
Gestation Length ◽  
Treatment Groups ◽  
Timed Artificial Insemination ◽  
Synchronization Protocol

Two synchronization protocols for fixed-timed AI (FTAI) in White-tail deer were evaluated over a 2-year period. InYear 1, White-tail does (n = 38), with a mean body weight of 55 kg and mean age of 2.4 years, were stratified by weight, age, and last fawning date randomly across 2 estrous cycle synchronization treatment groups. Does received either a CIDR-G device for 14 days (CIDR 14) and underwent AI 60 h post-CIDR removal, or received a CIDR-G device for 7 days (CIDR 7) with 1 mg of estradiol benzoate (i.m.) at CIDR insertion (Day 0), 25 mg of prostaglandin F2α (PGF2α; Dinoprost) on Day 6 and 1 mg of estradiol benzoate (i.m.) on Day 7 with AI occurring 52 h post-CIDR removal. In Year 2, White-tail does (n = 36), with a mean body weight of 60 kg and mean age of 3.9 years, were stratified as described for Year 1 and allotted to 2 treatments. Does received a CIDR-G device for 14 days and were inseminated 60 h post-CIDR removal (Treatment A) or were synchronized in the same way as does inTreatment A but also received 200 IU (i.m.) of eCG (Sigma, St. Louis, MO) at CIDR removal (Treatment B). All does received 1 mg of Domosedan (i.v.) before initiating the AI procedure. In both years, electroejaculated semen was collected from a single buck and frozen for AI. In both years, clean-up bucks were introduced into the pen with does for natural cover at no less than 14 days following AI. A gestation length of 195 � 7 days was used to determine whether the fawn(s) resulted from AI or from natural mating. In Year 1, FTAI pregnancy rates were not different between the CIDR 14 (56%) and CIDR 7 (24%) treatment groups. Likewise, pregnancy rates were not different between CIDR 14 FTAI (56%) and clean-up bucks (75%). However, the FTAI pregnancy rate was lower (P < 0.001) for the CIDR 7 treatment group (24%) compared with the clean-up bucks (100%). Treatments did not affect fecundity rates; however, those females pregnant from FTAI had lower (P < 0.001) fecundity rates compared with those females pregnant from natural cover (1.2 v. 1.9 fawns/doe). In Year 2, treatment did not affect FTAI pregnancy rates across treatments (33% for Treatment A and 55% Treatment B); however, fewer (P = 0.024) does in Treatment A were pregnant following FTAI (33%) compared with those pregnant from clean-up bucks (75%). There was no difference in FTAI fecundity rates across treatment groups (1.3 v. 1.7 for Treatments A and B, respectively) or between FTAI treatments and natural cover (1.3, 1.7, and 1.5 for Treatments A, B, and clean-up bucks, respectively). These results indicate that the use of a 14-day CIDR synchronization protocol with FTAI occurring 60 h after CIDR removal yields acceptable pregnancy rates of White-tail does following transcervical AI.

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