scholarly journals Thrombolytic and Cytotoxic Activities of Terminalia bellerica Roxb.

2015 ◽  
Vol 16 (2) ◽  
pp. 131-135
Author(s):  
M Sekendar Ali ◽  
Kazi Omar Faruq ◽  
Atiqul Islam ◽  
AMM Nurullah ◽  
Kazi Ashfak Ahmed Chowdhury ◽  
...  
Keyword(s):  
Brine Shrimp ◽  
Thrombolytic Agent ◽  
Methanolic Extract ◽  
Pharmaceutical Journal ◽  
Cytotoxic Activities ◽  
Cytotoxic Potential ◽  
Thrombolytic Activity ◽  
Brine Shrimp Lethality ◽  
Terminalia Bellerica ◽  
Thrombolytic Effect

The present study was designed to investigate the thrombolytic activity and cytotoxic potential of the methanolic extract of bark of Terminalia bellerica Roxb. The cytotoxicity was assessed with the brine shrimp lethality bioassay and thrombolytic effect with human blood. The brine shrimp lethality bioassay was used to evaluate cytotoxicity (LC50 = 3.21 ?g/ml) compared to vincristine of sulphate (LC50 = 0.512 ?g /ml). It was also evaluated as thrombolytic agent as compared to streptokinase. It has significant thrombolytic activity (32.95%) compared to standard streptokinase (70%). DOI: http://dx.doi.org/10.3329/bpj.v16i2.22293 Bangladesh Pharmaceutical Journal 16(2): 131-135, 2013

scholarly journals Phytochemicals, Antimicrobial and Cytotoxic Potential Study of Tinospora cordifolia

2020 ◽  
pp. 77-84
Author(s):  
Anik Barua ◽  
Md. Rabiul Hossain ◽  
Labonno Barua ◽  
Abu Sadat Md. Sayem ◽  
Md. Zainul Abedin ◽  
...  
Keyword(s):  
Brine Shrimp ◽  
Methanolic Extract ◽  
Antimicrobial Property ◽  
Biological Properties ◽  
Tinospora Cordifolia ◽  
Diffusion Method ◽  
Cytotoxic Potential ◽  
Brine Shrimp Lethality ◽  
Brine Shrimp Lethality Assay ◽  
Stem Extract

Aims: Tinospora cordifolia is a herb used in the traditional folk medicine due to their health benefits. Studies reported the biochemical composition and some biological properties of the plant stem. The aim of the current study was to investigate the antimicrobial and cytotoxic properties from the extract of Tinospora cordifolia stem. Place and Duration of Study: The study was accomplished by eleven months in the Department of Pharmacy, University of Science and Technology Chittagong (USTC), Bangladesh.  Methodology: Presence of phytochemical constituents such as alkaloids, flavonoids and tannins, saponins were evaluated. Antimicrobial activity of the stem extract was assayed separately using an agar diffusion method against some gram negative and gram positive bacteria. The cytotoxic potential of the stem extract was evaluated by the Brine-Shrimp Lethality Assay mathod.  Results: The zone of inhibition formed by the methanolic extract in the antimicrobial assays was significant. In addition, cytotoxicity of the extract was found to be significant as compared to the standard gallic acid in Brine-Shrimp lethality assay. Conclusion: The methanolic extract of Tinospora cordifolia may possess significant antimicrobial property which requires more studies to isolate the specific bioactive compound for developing a new antimicrobial agent from this plant.

scholarly journals Leaves of Clerodendrum wallichi Merr. Exhibit Analgesic, Antioxidant and Cytotoxic Activities

2019 ◽  
Vol 22 (2) ◽  
pp. 208-213
Author(s):  
Tufael Ahmed ◽  
Farah Ashrafi Noor ◽  
Mahfuza Afroz Soma ◽  
Fahima Aktar ◽  
Md Ruhul Kuddus
Keyword(s):  
Acetic Acid ◽  
Brine Shrimp ◽  
Methanolic Extract ◽  
Radical Scavenging ◽  
Pharmaceutical Journal ◽  
Total Phenol Content ◽  
Tail Flick ◽  
Cytotoxic Activities ◽  
Traditional Use ◽  
Scavenging Capacity

The present study was conducted to evaluate the analgesic, antioxidant and cytotoxic activities of leaves of Clerodendrum wallichii Merr. The analgesic activity of the crude methanolic extract of C. wallichii (MECW) was determined by tail flick- and acetic acid-induced writhing method in Swiss albino mice. In tail flick method, oral administration of MECW at 400 mg/kg body weight exhibited 362.19% elongation of pain response in experimental mice at 60 min. The same dose of plant extract significantly (p < 0.05) inhibited the acetic acid-induced abdominal constrictions in mice and this effect was dose-dependent. In addition, the methanol extract of C. wallichii (MECW) and its petroleum-ether (PESF), chloroform (CSF), dichloromethane (DCMSF) and aqueous (AQSF) soluble fraction were subjected to assay for determination of total phenol content, antioxidant and cytotoxic activities test by standard protocols. The highest amount of phenolic content was present in the MECW (75.44±0.68 mg of GAE/g of extract) while the AQSF displayed the highest free radical scavenging capacity (IC50 value of 7.23±0.05 μg/ml) as compared to 25.18±1.89 μg/ml exhibited by the standard butylated hydroxyl toluene (BHT). In brine shrimp lethality test, the number of surviving shrimp nauplii was counted and LC50 was assessed after 24 h. Both MECW and its PESF exhibited maximum toxicity towards brine shrimp with LC50 value of 10.3±0.01 μg/ml with respect to standard vincristine sulfate (having LC50 of 0.45 μg/ml). The results of the present investigations suggest that methanolic extract of C. wallichii possesses analgesic, antioxidant and cytotoxic activities, confirming the traditional use of the plant. Bangladesh Pharmaceutical Journal 22(2): 208-213, 2019

scholarly journals Evaluation of antioxidant activity and cytotoxic potential of Cryp-tocoryne ciliata

2013 ◽  
Vol 2 (2) ◽  
pp. 38-41
Author(s):  
Ummey Jannatun Nahar ◽  
Shah Marzia Mahjabin Lina
Keyword(s):  
Antioxidant Activity ◽  
Carbon Tetrachloride ◽  
Ethyl Acetate ◽  
Brine Shrimp ◽  
Methanolic Extract ◽  
Soluble Fraction ◽  
Cytotoxic Activities ◽  
Brine Shrimp Lethality ◽  
Whole Plant ◽  
Vincristine Sulphate

Cryptocoryne ciliata belonging to the Araceae family has been investigated for isolation of its secondary metabolites and evaluation of biological activities of the crude extractives with special emphasis to the antioxidant activity and brine shrimp lethality bioassay. The whole plant was extracted with methanol and concentrated extract was partitioned using petroleum ether, carbon tetrachloride and ethyl acetate. Aqueous soluble fraction of the methanolic extract showed the highest antioxidant activity. The carbon tetrachloride soluble fraction of the methanolic extract and the ethyl acetate soluble fraction of the methanolic extract showed moderate antioxidant activity as compared to free antioxidant activity of tert-butyl-1-hydroxytolunene. In the brine shrimp lethality bioassay, among all extracts of whole plant of C. ciliata, the carbon tetrachloride soluble fraction of the methanolic extract showed strong cytotoxic activity. Aqueous soluble fraction of the methanolic extract, methanolic crude and ethyl acetate soluble fraction of the methanolic extract showed mild cytotoxity as compared to that of vincristine sulphate. The study confirms the mild to moderate antioxidant and moderate potent cytotoxic activities of C. ciliata plant extract as compared to reference standards and therefore demands the isolation of active principles and thorough bioassay.DOI: http://dx.doi.org/10.3329/icpj.v2i2.13196 International Current Pharmaceutical Journal 2013, 2(2): 38-41

scholarly journals In vitro Evaluation of Cytotoxic and Thrombolytic Activities of Oroxylum indicum (Linn.)

2015 ◽  
Vol 17 (1) ◽  
pp. 70-74 ◽  
Author(s):  
Md Rabiul Islam ◽  
ASM Ali Reza ◽  
Md Sajjad Hossain ◽  
Mst Kaniz Farhana
Keyword(s):  
Brine Shrimp ◽  
Pharmaceutical Journal ◽  
Negative Control ◽  
Flowering Plant ◽  
Clot Lysis ◽  
Cytotoxic Activities ◽  
Oroxylum Indicum ◽  
Vincristine Sulphate ◽  
Thrombolytic Effect

Oroxylum indicum is a species of flowering plant belonging to the family Bignoniaceae and have many medicinal properties which prompted us to evaluate its possible thrombolytic and cytotoxic activities. A quick and rapid methodology was applied to find out the thrombolytic effect of the methanol extract of leaves of O. indicum. Streptokinase and water were employed as a positive and negative control, respectively. Thrombolytic effect was expressed as percentage of clot lysis. O. indicum, streptokinase and water demonstrated 23.69 ± 6.97%, 78.41 ± 0.366% and 5.19 ± 0.241% clot lysis, respectively. Cytotoxic property was evaluated by the brine shrimp lethality bioassay and the result was expressed as LC50. In this study, DMSO was used as solvent and vincristine sulphate as positive control. The extract showed cytotoxicity against brine shrimp nauplii and the calculated LC50 of O. indicum and vincristine sulphate were 251.2 ?g/ml and 5.2 ?g/ml, respectively. Due to the cytotoxic and thrombolytic activities, O. indicum may be regarded as a promising candidate for natural anticancer agent and should be subjected to purification processes for useful therapeutic drugs. DOI: http://dx.doi.org/10.3329/bpj.v17i1.22319 Bangladesh Pharmaceutical Journal 17(1): 70-74, 2014

scholarly journals Evaluation of Antidiarrheal Activity of Methanolic Extract ofMaranta arundinaceaLinn. Leaves

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Md. Khalilur Rahman ◽  
Md. Ashraf Uddin Chowdhury ◽  
Mohammed Taufiqual Islam ◽  
Md. Anisuzzaman Chowdhury ◽  
Muhammad Erfan Uddin ◽  
...  
Keyword(s):  
Brine Shrimp ◽  
Castor Oil ◽  
Methanolic Extract ◽  
Cytotoxic Activities ◽  
Pharmacological Effects ◽  
Standard Drug ◽  
Brine Shrimp Lethality ◽  
Common Causes ◽  
Diarrheal Episode ◽  
Antidiarrheal Activity

Diarrhea is one of the most common causes for thousands of deaths every year. Therefore, identification of new source of antidiarrheal drugs becomes one of the most prominent focuses in modern research. Our aim was to investigate the antidiarrheal and cytotoxic activities of methanolic extract ofMaranta arundinacealinn. (MEMA) leaves in rats and brine shrimp, respectively. Antidiarrheal effect was evaluated by using castor oil-induced diarrhea, enteropooling, and gastrointestinal motility tests at 200 mg/kg and 400 mg/kg body weight in rats where the cytotoxic activity was justified using brine shrimp lethality bioassay at different concentrations of MEMA. The extract showed considerable antidiarrheal effect by inhibiting 42.67% and 57.75% of diarrheal episode at the doses of 200 and 400 mg/kg, respectively. MEMA also significantlyp<0.01reduced the castor oil-induced intestinal volume (2.14 ± 0.16 to 1.61 ± 0.12 mL) in enteropooling test as well as intestinal transit (33.00 to 43.36%) in GI motility test, compared to their respective control. These observed effects are comparable to that of standard drug loperamide (5 mg/kg). On the other hand, in brine shrimp lethality test after 24 h, surviving brine shrimp larvae were counted and LD50was assessed. Result showed that MEMA was potent against brine shrimp with LD50value of 420 µg/mL. So the highest dose of 400 µg/mL of MEMA was not toxic to mice. So these results indicate that bioactive compounds are present in methanolic extract ofMaranta arundinacealeaves including significant antidiarrheal activity and could be accounted for pharmacological effects.

scholarly journals Phytochemical and Biological Investigations of Eurya acuminata (Theaceae)

2017 ◽  
Vol 15 (2) ◽  
pp. 151-154 ◽  
Author(s):  
Tahmid Faisal ◽  
Monira Ahsan ◽  
Jakir Ahmed Choudhury ◽  
ATM Zafrul Azam
Keyword(s):  
Brine Shrimp ◽  
High Field ◽  
Methanol Extract ◽  
Methanolic Extract ◽  
Antimicrobial Properties ◽  
Spectroscopic Studies ◽  
Vincristine Sulfate ◽  
Thrombolytic Activity ◽  
Aqueous Fraction ◽  
Lc50 Value

The methanol extract of the powdered leaf of Eurya acuminata was investigated for isolation of secondary metabolites and two compounds were obtained by using VLC, column chromatography and TLC. The compounds were identified as phytol (1) and ?-sitosterol by extensive spectroscopic studies, including high field NMR analyses as well as co-TLC with authentic samples. The methanol extract of leaf of E. acuminata and its organic and aqueous soluble partitioning materials were evaluated for cytotoxic, thrombolytic and antimicrobial properties. In the cytotoxicity study the aqueous fraction of crude methanolic extract showed significant lethality towards brine shrimp having LC50 value 8.821 ?g/ml as compared to standard vincristine sulfate (0.404 ?g/ml). In the study for thrombolytic property, different extract of E. acuminata exhibited various thrombolytic activity ranging from 13.66 to 31.89 % as compared to standard streptokinase (46.51 %). No antimicrobial activity was observed from leaf extracts.Dhaka Univ. J. Pharm. Sci. 15(2): 151-154, 2016 (December)

scholarly journals Steroids from the Leave of Persicaria perfoliata (L.) H. Gross

2019 ◽  
Vol 22 (2) ◽  
pp. 224-227
Author(s):  
Md Sagar Hossain ◽  
NM Mofiz Uddin Khan ◽  
Md Saiful Quddus ◽  
AM Sarwaruddin Chowdhury
Keyword(s):  
Antimicrobial Activity ◽  
Cytotoxic Activity ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Soluble Fraction ◽  
Pharmaceutical Journal ◽  
Antimicrobial Screening ◽  
Brine Shrimp Lethality ◽  
Persicaria Perfoliata ◽  
Test Organisms

β-sitosterol (1) and β-sitosterol-D-glucoside (2) were isolated from the dichloromethane soluble fraction of a methanol extract of the leave of Persicaria perfoliata. The crude fraction was subjected to antimicrobial screening and brine shrimp lethality bioassay, where moderate antimicrobial activity was observed against most of the test organisms was seen. The hexane soluble fraction also displayed significant cytotoxic activity with LC50 0.64 μg/ml in brine shrimp lethality bioassay. This is the first report of occurrence of these compounds from this plant. Bangladesh Pharmaceutical Journal 22(2): 224-227, 2019

scholarly journals Preliminary cytotoxic activity of different extracts of Averrhoa bilimbi (fruits)

2013 ◽  
Vol 2 (3) ◽  
pp. 83-84 ◽  
Author(s):  
Md. Ramjan Ali ◽  
Marjan Hossain ◽  
Jannatul Ferdous Runa ◽  
Md. Hasanuzzaman
Keyword(s):  
Carbon Tetrachloride ◽  
Room Temperature ◽  
Methanolic Extract ◽  
Pharmaceutical Journal ◽  
Artemia Salina ◽  
Active Principle ◽  
Cytotoxic Potential ◽  
Potential Source ◽  
Soluble Fractions

The crude methanolic extract of Averrhoa bilimbi Linn. (Oxalidiaceae) fruits and its different fractions have been investigated for the evaluation of in vitro cytotoxic potential. The dried and powder fruits were extracted with methanol at room temperature and the concentrated methanolic extract was fractionated by the modified Kupchan partitioning method to provide pet-ether, carbon tetrachloride, chloroform and aqueous soluble fractions. Brine shrimp (Artemia salina) lethality bioassay was used to investigate the cytotoxic potential of A. bilimbi. Compared to vincristine sulfate (with LC50 of 0.839 µg/ml) methanolic extract, carbon tetrachloride and pet-ether soluble fractions demonstrated a significant cytotoxic potential (having LC50 of 0.005µg/ml, 1.198µg/ml and 0.781µg/ml, respectively). The LC50 values of chloroform and aqueous soluble fractions were 5.691µg/ml and 6.123µg/ml, respectively. This study reveals that A. bilimbi possesses effective cytotoxic properties and hence can be a potential source for the isolation of active principle(s) for cancer therapy.DOI: http://dx.doi.org/10.3329/icpj.v2i3.13634 International Current Pharmaceutical Journal, February 2013, 2(3): 83-84 

scholarly journals Anthelmintic and Cytotoxic Activities of Two Medicinal Plants: Polygonum viscosum and Aphanamixis polystachya Growing in Bangladesh

2014 ◽  
Vol 6 (2) ◽  
pp. 339-345 ◽  
Author(s):  
M. N. Amin ◽  
M. S. Majumder ◽  
M. M. R. Moghal ◽  
S. Banik ◽  
A. Kar ◽  
...  
Keyword(s):  
Methanolic Extract ◽  
Anthelmintic Activity ◽  
Cytotoxic Activities ◽  
Dependent Manner ◽  
Reference Standard ◽  
Cytotoxic Potential ◽  
Lc50 Value ◽  
Vincristine Sulphate ◽  
Dose Dependent

The present study was designed to investigate in vitro anthelmintic and cytotoxic activities of crude methanolic extract of two plants(Polygonum viscosum and Aphanamixis polystachya) grown in Bangladesh. Evaluation of cytotoxic activity was done using the brine shrimp lethality bioassay. The crude methanolic extract of Polygonum viscosum showed significant cytotoxic potential (LC50 value of 6.34 ?g/ml) among all the fractions comparing with that of standard vincristine sulphate (0.825 ?g/ml). Besides, the LC50 values of crude methanolic extract, pet ether and chloroform extracts of Aphanamixis polystachya showed good cytotoxic activities 11, 10.36, and 16.45 µg/ml, respectively. The other study was undertaken to evaluate anthelmintic activity (using Pheretima posthuma model) where piperazine was used as reference standard. The crude methanolic extract of Polygonum viscosum leaves produced a significant anthelmintic activity in dose dependent manner and the activity of crude extract was comparable with that of standard drugs. Besides, the Aphanamixis polystachya extract revealed moderate anthelmintic activity. Here, the anova testing was done with the P < 0.05. Further studies are suggested to determine the active compounds responsible for the anthelmintic and cytotoxic activities of these two plant extracts.   Keywords: Anthelmintic; Cytotoxic; Medicinal plant; Aphanamixis polystachya; Polygonum viscosu.  © 2014 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.   doi: http://dx.doi.org/10.3329/jsr.v6i2.17299 J. Sci. Res. 6 (2), 339-345 (2014) 

scholarly journals 6. Cytotoxic Potential of n-Hexane Extract of Calotropis gigantea L. Leaves

2016 ◽  
Vol 1 (1) ◽  
pp. 45-49 ◽  
Author(s):  
Kartini Hasballah ◽  
Murniana Murniana ◽  
Al Azhar ◽  
Nurul Rahmi
Keyword(s):  
Liquid Chromatography ◽  
Crude Extract ◽  
Brine Shrimp ◽  
Hexane Extract ◽  
Phytochemical Screening ◽  
Cytotoxic Potential ◽  
Calotropis Gigantea ◽  
Brine Shrimp Lethality ◽  
Vacuum Liquid Chromatography

The present study was done to determine the cytotoxic potential of n-hexane extract of Calotropis gigantea L. leaves and its fractions. Here, dried leaves of C. gigantea L. were macerated using n-hexane to obtain crude extract of 21.16 g (1.03%). The components of n-hexane extract of C. gigantea L. leaves were separated with vacuum-liquid chromatography resulted 4 fractions which are A (0.5 g), B (0.9g), C (5.29 g), and D (6.25 g). Phytochemical screening indicated that the extract contained various secondary metabolic compounds such as steroids, terpenoids, saponins, flavonoids, coumarins, and phenolics. Cytotoxic potenital of the crude extract of C. gigantea L. and its fractions was examined using brine shrimp lethality test (BSLT). The LC50 values of C. gigantea’s n-hexane extract and its fractions that determined by Finney method were 272.27 (n-hexane extract), 31.62 (fraction A), 43.65 (fraction B), 33.89 (fraction C) and 20.98 (fraction D) ?g/mL, respectively. The most active was D fraction contained terpenoids, flavonoids, and phenolics which were assumed contribute to its cytotoxic potential. These results suggested that n-fraction of C. gigantea might possess antitumor or pesticidal activities..

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