scholarly journals Microbiological analysis of raw milk, pasteurized milk and yogurt samples collected from different areas of Dhaka city, Bangladesh

2015 ◽  
Vol 39 (1) ◽  
pp. 31-36 ◽  
Author(s):  
Tasmina Rahman ◽  
Tanzia Akon ◽  
Iftikharun Nessa Sheuli ◽  
Naima Hoque
Keyword(s):  
Listeria Monocytogenes ◽  
Bacterial Count ◽  
Raw Milk ◽  
Total Coliform ◽  
Microbiological Analysis ◽  
Dhaka City ◽  
Salmonella Spp ◽  
Pasteurized Milk ◽  
Milk Samples ◽  
Shigella Spp

A total of 300 different milk and yogurt samples were collected from Dhaka city and analyzed for total viable bacterial count (TVBC), total coliform count (TCC) and presence of several pathogens such as Escherichia coli, Salmonella spp., Shigella spp., Vibrio spp. and Listeria monocytogenes. Raw milk samples collected from Moghbazar area showed highest TVBC (4.2 × 106 cfu/ml) and samples from Mohammadpur area showed lowest count (3.5 × 103 cfu/ml). All the pasteurized milk samples showed TVBC of 1.9 × 102 to 2.8 × 103 cfu/ml, and TCC only in one sample Yogurt samples (open) collected from vendor showed TVBC of 9.1 × 103 to 8.2 × 107 cfu/ml, and TCC only in sample collected from Dhanmondi, Dhaka. The packed yogurt samples showed TVBC of 2.2 × 102 to 6.1 × 103 cfu/ml, and TCC only in one sample. All the milk and yogurt samples were found contaminated with E. coli and Shigella-like species. Listeria monocytogenes was not detected in any of the samples studied.Journal of Bangladesh Academy of Sciences, Vol. 39, No. 1, 31-36, 2015

scholarly journals Microbiological analysis of different categories of food items in Dhaka city, Bangladesh

Food Research ◽  
2020 ◽  
Vol 4 (4) ◽  
pp. 1351-1356
Author(s):  
M.A. Uddin ◽  
T. Jabin ◽  
E.A. Siam ◽  
M.T. Afreen ◽  
S.S. Uddin ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Microbiological Quality ◽  
Bacterial Count ◽  
Food Samples ◽  
Microbiological Analysis ◽  
Dhaka City ◽  
Biochemical Tests ◽  
Salmonella Spp ◽  
Wide Range ◽  
Shigella Spp

Pathogenic microorganisms have been so far reported to contaminate a wide range of foods triggering food borne infections or intoxications including the enteric complications, abdominal pain, fever, bloodstream infection etc. The current study was attempted to observe the microbiological quality of some popular foods collected from different places of Dhaka city, Bangladesh. Conventional cultural, microscopic and biochemical tests were followed for the detection and enumeration of bacterial isolates associated with these food samples. The investigation encompassed detection of total viable bacterial count (TVBC) and presumptive identification of other pathogenic bacteria from these samples. Higher counts of TVBC, coliform, Staphylococcus spp. and fungal load were recorded as 1.46×107 CFU/g (yogurt 1), 4.5×106 CFU/g (yogurt 1), 5.6×105 CFU/g (raw meat) and 2.9×103 CFU/g (sea fish), respectively. Fecal coliform was detected only in one out of ten samples. On the contrary Salmonella spp., Vibrio spp., Shigella spp. and Pseudomonas spp. were completely absent. The antibiogram study showed that all the isolates were sensitive against Kanamycin and Azithromycin. Better sensitivity was also observed against Gentamycin and Ciprofloxacin. Varying degree of antibiotic resistance was also detected against Cefixime, Amikacin and Neomycin. Our study emphasizes the need for continuous monitoring of the various categories of food samples for the safety of public health.

scholarly journals Microbiological quality analysis of raw, pasteurized, UHT milk samples collected from different locations in Bangladesh

2015 ◽  
Vol 4 (1) ◽  
pp. 5-8 ◽  
Author(s):  
Sourav Kumar Banik ◽  
Kamal Kanta Das ◽  
Md Aftab Uddin
Keyword(s):  
Microbiological Quality ◽  
Bacterial Count ◽  
Raw Milk ◽  
Total Coliform ◽  
Point Of View ◽  
Quality Analysis ◽  
Expiry Date ◽  
Pasteurized Milk ◽  
Milk Samples

Present study attempted to determine the microbiological quality of raw, pasteurized and UHT (Ultra High Temperature-processed) milk samples collected from different locations in Bangladesh. A total of 46 samples were studied including 22 raw milk samples from the local dairy markets and 24 different brands of pasteurized and ultrahigh temperature (UHT) treated milk manufactured in different beverage industries. The samples were examined for determining the total viable bacterial count (TVBC) and total coliform count (TCC). Results revealed that the raw milk samples were substandard in terms of TVBC and TCC. The range of TVBC and TCC in raw milk samples was 5.2×108 to 1.3×107 cfu/ml and 4.2×104 to 1.0×104 cfu/ml, respectively. On the contrary, the quality of pasteurized and UHT-treated milks was excellent. The TVBC range in pasteurized milk samples was from 1.8×103 to 1.1×102 cfu/ml, slightly lower than that recommended by the Bangladesh Standards and Testing Institution (BSTI). Interestingly sample numbers P-6, P-10 and P-12 of pasteurized milk samples had no growth at all both in terms of TVBC and TCC and none of the UHT processed milk contained any bacteria. So from the consumer point of view, both types of processed milk can be considered safe for consumption within the mentioned expiry date. DOI: http://dx.doi.org/10.3329/sjm.v4i1.22753 Stamford Journal of Microbiology, Vol.4(1) 2014: 5-8

scholarly journals Isolation and Identification of Pathogenic Escherichia coli, Klebsiella spp. and Staphylococcus spp. in Raw Milk Samples Collected from Different Areas of Dhaka City, Bangladesh

1970 ◽  
Vol 1 (1) ◽  
pp. 19-23 ◽  
Author(s):  
Md Aftab Uddin ◽  
Hasan Md Motazzim-ul-Haque ◽  
Rashed Noor
Keyword(s):  
Escherichia Coli ◽  
Nalidixic Acid ◽  
Pathogenic Bacteria ◽  
Bacterial Count ◽  
Raw Milk ◽  
Total Coliform ◽  
Resistance Pattern ◽  
Dhaka City ◽  
Isolation And Identification ◽  
Milk Samples

The present study was undertaken with the aim of investigating the bacteriological quality of locally available raw milk. A total number of 22 raw milk samples were collected from Dhaka city and its surrounding areas during the period from October 2009 to November 2009. The analysis comprised enumeration of total viable bacterial count (TVBC), total coliform count (TCC) and total staphylococcal count (TSC) for the determination of sanitary quality. The highest TVBC, TCC and TSC were 2.36 x109 cfu/ml, 2.0 x 108 cfu/ml & 4.7 x 107 cfu/ml, respectively. In order to observe the antibiotic-resistance pattern, the antibiogram assay was carried out. All Escherichia coli isolated from raw milk exhibited 100% resistance against Rifampin (R) and Tetracycline (TE) and 50% resistance against Nalidixic Acid (NA) but were 100% sensitive against Imipenem (IPM). Klebsiella spp. exhibited 100% sensitivity against Imipenem (IPM). Staphylococcus spp. isolated from raw milk exhibited 100% resistance against Rifampin (R) and Nalidixic Acid (NA), but were 100% sensitive to Gentamicin (CN) and Imipenem (IPM). This survey indicates that most of the raw milk samples were not satisfactory in course of public health standard as some pathogenic bacteria were detected from these samples. Frequent use of antibiotics should be prohibited since antibiotic resistant strains are continuously increasing.DOI: http://dx.doi.org/10.3329/sjm.v1i1.9098  Stamford Journal of Microbiology, Vol.1(1), July 2011, p.19-23

scholarly journals Assessment of listeria monocytogenes, staphylococcus aureus and salmonella SPP. Contamination in dairy samples collected in Gia Lam and Ba Vi, Hanoi early 2019

2019 ◽  
Vol 2 (4) ◽  
pp. 22-32
Author(s):  
Minh Huyen Nguyen Thi ◽  
Hoa Tran Thi ◽  
Tuyet Lan Ninh Thi ◽  
Hien Tran Thi ◽  
◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Listeria Monocytogenes ◽  
Dairy Products ◽  
Pathogenic Bacteria ◽  
Raw Milk ◽  
Accurate Method ◽  
Salmonella Spp ◽  
Pasteurized Milk ◽  
Milk Samples ◽  
Milk And Dairy Products

Milk and dairy products from dairy farms around Hanoi greatly contribute to the consumed milk quantity in Hanoi. The use of fresh milk or pasteurized milk becomes more and more popular in the daily life of local people. Milk and dairy products were widely sold in numerous stores, particularly in Xuan Mai, Ba Vi, Phu Dong and Gia Lam. However, there have not yet been any studies to assess the pathogenic bacterial contamination of these products. In our study, 49 samples including 23 raw milk samples, 12 pasteurized milk samples, and 14 yogurt samples were collected in order to examine the presence of food­born pathogenic bacteria such as Listeria monocytogenes, Staphylococcus aureus, Salmonella spp. using PCR method. This fast and accurate method works based on the specific amplification of tested bacterial DNA. The results showed that one of the samples may contain Staphylococcus aureus while another may be contaminated with Listeria monocytogenes. None of the samples was contaminated with Salmonella spp. The results were confirmed by gene sequencing.

scholarly journals Isolation and quantification of indicator and pathogenic microorganisms along with their drug resistance traits from bottled and jar water samples within Dhaka city, Bangladesh

2020 ◽  
Vol 9 (1) ◽  
pp. 12-14
Author(s):  
Md Aftab Uddin ◽  
Mst Aysha Siddiqua ◽  
Mst Sadia Ahmed
Keyword(s):  
Water Samples ◽  
Bacterial Count ◽  
Fecal Coliforms ◽  
Pathogenic Microorganisms ◽  
Dhaka City ◽  
Bacterial Isolates ◽  
Salmonella Spp ◽  
Potential Threat ◽  
Antibiotic Susceptibility Pattern ◽  
Shigella Spp

Commercial drinking water may serve as potential threat to public health if these items are contaminated with a number of pathogenic microorganisms due to faulty manufacturing process. Present study attempted to isolate and quantify the microorganisms from various jar and bottle water samples collected from various areas of Dhaka city. Antibiotic susceptibility pattern of suspected bacterial isolates were also determined in this study. Out of the eighteen samples studied, ten were jar water samples and eight were bottled water samples. The range of total viable bacterial count (TVBC) in these samples ranged from 102 to 105 cfu/ml. Specific pathogens such as, Salmonella spp., Shigella spp., Vibrio spp. and fecal coliforms could not be found in these samples. However coliforms could be detected in 10 samples. The antibiogram study showed that all Escherichia coli and Klebsiella spp. isolates found from these samples were sensitive against gentamicin (10 μg) and azithromycin (30 μg). Variable antibiotic resistance among these bacterial isolates was detected against cefotaxime (30 μg), streptomycin (10 μg) and erythromycin (15 μg). Stamford Journal of Microbiology, Vol.9(1) 2019: 12-14

scholarly journals Microbiological Profile of the Traditionally Collected Industrial Raw Milk from the Milk Pocket Zones of Bangladesh

1970 ◽  
Vol 25 (1) ◽  
pp. 17-20 ◽  
Author(s):  
Gouranga C Chanda ◽  
Gazi M Noor Uddin ◽  
Aparna Deb ◽  
Tahmina Bilkis ◽  
Sharmin Chowdhury ◽  
...  
Keyword(s):  
Bacterial Count ◽  
Raw Milk ◽  
Total Coliform ◽  
Plate Count ◽  
Coliform Count ◽  
Standard Plate Count ◽  
Milk Samples ◽  
Bacteriological Profile ◽  
Standard Plate ◽  
Viable Bacteria

The study was aimed to evaluate the bacteriological profile of the traditionally collected industrial raw milk from the milk pocket zones of Bangladesh. About 365 raw milk samples were collected from the milk tanker, who brought raw milk from the mother chilling centre where raw milk was chilled at 4°C following traditional method. All milk samples were subjected to perform standard plate count and total coliform count. The average standard plate count was found to be 4.37 x 106 cfu/ml and the highest occurrence of standard plate count was found to be 6.70 x 106 cfu/ml in October and the lowest (3.28 x 106 cfu/ml) in March. The highest occurrence of total viable bacteria was found to be 5.64 x 106 cfu/ml in autumn and the lowest was found to be 3.78 x 106 cfu/ml in summer. On the other hand, the average of the coliform bacterial count was found to be 3.88 x 105 cfu/ml with the highest (5.70 x 105 cfu/ml) occurrence in May and the lowest (1.90 x 105 cfu/ml) in January. Moreover, the highest occurrence of coliform count was found to be 4.84 x 105 cfu/ml in rainy season and the lowest was 2.75 x 105 cfu/ml found in winter.DOI: http://dx.doi.org/10.3329/bjm.v25i1.4849 Bangladesh J Microbiol, Volume 25, Number 1, June 2008, pp 17-20

scholarly journals Microbiological analysis and antibacterial activity of pear samples

2016 ◽  
Vol 5 (1) ◽  
pp. 1-4 ◽  
Author(s):  
Habiba Afroz ◽  
Tasnia Ahmed ◽  
Md Aftab Uddin
Keyword(s):  
Fungal Growth ◽  
Bacterial Count ◽  
Total Coliform ◽  
Microbiological Analysis ◽  
Salmonella Spp ◽  
Pseudomonas Spp ◽  
Two Samples ◽  
And Staphylococcus Aureus ◽  
Klebsiella Spp ◽  
Vibrio Spp

Present study attempted to isolate and quantify the microorganisms from the pear samples collected within Dhaka metropolis. Out of the five samples studied, total viable bacterial count and the total coliform count were near about 105 cfu/g and 104 cfu/g, respectively. Among the specific pathogens, prevalence of Salmonella spp. and Vibrio spp. were found only in two samples. Whereas Klebsiella spp, Pseudomonas spp. and Staphylococcus aureus were detected in all the samples studied. Fungal growth was also observed in each sample within the range of 8.8×104 – 9.5×104 cfu/g. The study of antibiogram revealed that, most of the pathogens were resistant to more than one antibiotic. Interestingly the pear samples showed no anti-bacterial activity against any of the laboratory isolates such as, Escherichia coli, Pseudomonas spp., Salmonella spp. and Bacillus spp.Stamford Journal of Microbiology, Vol.5(1) 2015: 1-4

scholarly journals Microbial analysis of raw and pasteurized milk from selected areas of Dinajpur, Bangladesh

2015 ◽  
Vol 1 (2) ◽  
pp. 292-296 ◽  
Author(s):  
Md Amirul Hasan ◽  
Md Aminul Islam ◽  
Mohammad Showkat Mahmud ◽  
ASM Ashab Uddin ◽  
Shamim Ahmed
Keyword(s):  
Microbial Contamination ◽  
Raw Milk ◽  
Total Coliform ◽  
Viable Count ◽  
Hygienic Condition ◽  
Pasteurized Milk ◽  
Legal Standards ◽  
Milk Samples ◽  
Microbial Analysis ◽  
The Government

Milk is highly vulnerable to microbial contamination and consequently is easily perishable. The aim of the present study was microbial analysis of raw and pasteurized milk. For this, a total of 32 milk samples (12 from raw milk and 20 from pasteurized milk) were collected from 8 sources. Among them three were raw (R1 to R3) sources and rest five were pasteurized milk (P1 to P5) sources. Each of the collected samples was investigated during the period from May to December, 2012. All milk samples were subjected to total viable count (TVC), total coliform count (TCC), Total Staphylococcus count (TSC) and Gram’s staining to determine the loads of microbes in raw and pasteurized milk. Total viable counts (TVC) range of 12 raw milk samples (R, R2 and R3) were 1.3 x106 to 7.4 x105 cfu/ml. The presence of Escherichia coli in the raw milk samples were from 2.3 x102 to 9.4 x102 cfu/ml, but the presence of Staphylococcus were from 5.9 x102 to 7.9 x102 cfu/ml. Whereas, the range of TVC for five brands of pasteurized milk (P1, P2, P3, P4, and P5) were from 1.8 x104 to 9.8 x104 cfu/ml, TSC were from 2.8 x10 to 8.6 x102 cfu/ml and TCC from 1.01 x102 to 9.1 x10 cfu/ml. Therefore, it can be concluded that high counts of bacteria were found in raw milk and pasteurized milk. The government therefore should conduct frequent inspection of the marketed milks to check whether they meet the minimum legal standards and should monitor the overall hygienic condition surrounding the production and handling of milk. Realistic standards for the raw milks need to be devised and appropriate training should be given to the raw milk producers in hygienic handling of milk.Asian J. Med. Biol. Res. June 2015, 1(2): 292-296

scholarly journals Microbiological Study on Supply Water and Treated Water in Dhaka City

1970 ◽  
Vol 1 (1) ◽  
pp. 42-45 ◽  
Author(s):  
Mrityunjoy Acharjee ◽  
Farjana Rahman ◽  
Sadia Afrin Beauty ◽  
Farahnaaz Feroz ◽  
M Majibur Rahman ◽  
...  
Keyword(s):  
Water Samples ◽  
Total Coliform ◽  
Faecal Coliform ◽  
Microbiological Analysis ◽  
Dhaka City ◽  
Sample Collection ◽  
Biochemical Tests ◽  
Salmonella Spp ◽  
Treated Water ◽  
Two Samples

Water borne diseases are the major cause of morbidity and mortality in developing countries like Bangladesh, where disease outbreaks occur due to unsafe drinking water, inadequate sanitation and poor hygienic practices. The objective of this study was to evaluate the quality of water used for drinking as well as household purposes in Dhaka city, Bangladesh. Eighteen water samples were collected from different rivers and canals. Among those, two samples were treated with chlorine. Physico-chemical parameters (pH, salinity, turbidity, conductivity and dissolved oxygen) were monitored during the time of sample collection using portable meters. For microbiological analysis, water samples were collected in sterile screw capped bottle. All Experiments were carried out within 24 hours after collecting the samples. All the water sources were found to be contaminated with total coliform, faecal coliform, Escherichia coli, Klebsiella spp., Salmonella spp., Shigella spp., Vibrio cholerae, Aeromonas spp. and Fungi except treated water samples. Several biochemical tests were performed for confirmation of suspected organisms. According to these results, huge amount of total coliform and faecal coliform were observed in every sample and these amount were very much similar for other organisms. Treated water samples were found to be microbiologically accepted than untreated water samples. DOI: http://dx.doi.org/10.3329/sjm.v1i1.9132    Stamford Journal of Microbiology, Vol.1(1), July 2011, p.42-45

scholarly journals Comparative study of some indicator bacteria in cows and buffaloes raw milk in Al-Diwaniyah Province

2012 ◽  
Vol 11 (3) ◽  
pp. 46
Author(s):  
K. H. Abass
Keyword(s):  
Winter Season ◽  
Bacterial Count ◽  
Raw Milk ◽  
Total Coliform ◽  
Coliform Bacteria ◽  
Winter Period ◽  
City Center ◽  
Summer Period ◽  
Milk Samples ◽  
Significant Difference

The present study was carried out in AL-Diwaniyah city and it's different districts whichincludes (city center, Afak district and their regions, AL-hamza district and their regions, ALShamiadistrict and their regions and AL-Daghara regions and its villages) during a periodNovember 2010 to July 2010. A total of samples used in this study were 120 raw milksamples collected includes 60 samples for each species for (cows and buffaloes) for twodifferent season of the year (Winter and Summer), first start from (beginning November 2009 toend of the January 2010) ,and the second start from (beginning May to July 2010) The resultsof this study revealed the rising the averages of microbes contaminated raw milk samples ineach species of animals included in this study,where raw milk samples for cows recorded210 4.2х CFU/ml , buffaloes 214 12х CFU/ml,The highest averages of total Coliform bacterialcount were found in raw milk samples of cows 214 4.3х CFU/ml and less averages in rawsamples of buffaloes 214 6.1х CFU/ml . While averages of Staphylococcus aureas were highin raw milk samples of buffaloes 0.49 х216CFU/ml and less in raw milk samples of cows0. 214 61х CFU/ ml.While the averages of Streptococcus spp. were 0.37х 216CFU/ml, 0.38 21х6 CFU/ml, for cows and buffaloes respectively. Seasons of the year had an effect on average oftotal bacterial count studied for each sample of raw milk for all species of animals includedin this study.Where shown rising averages of microbes numbers during the summer period(May, Jun, and July), while there are decreasing in an average of microbes during the winter period(November, December, and January).Summer season recorded significant difference ascompared with winter season but averages of total coliform bacteria had no significantdifference between the two seasons.

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