scholarly journals Microbiological analysis of different categories of food items in Dhaka city, Bangladesh

Food Research ◽  
2020 ◽  
Vol 4 (4) ◽  
pp. 1351-1356
Author(s):  
M.A. Uddin ◽  
T. Jabin ◽  
E.A. Siam ◽  
M.T. Afreen ◽  
S.S. Uddin ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Microbiological Quality ◽  
Bacterial Count ◽  
Food Samples ◽  
Microbiological Analysis ◽  
Dhaka City ◽  
Biochemical Tests ◽  
Salmonella Spp ◽  
Wide Range ◽  
Shigella Spp

Pathogenic microorganisms have been so far reported to contaminate a wide range of foods triggering food borne infections or intoxications including the enteric complications, abdominal pain, fever, bloodstream infection etc. The current study was attempted to observe the microbiological quality of some popular foods collected from different places of Dhaka city, Bangladesh. Conventional cultural, microscopic and biochemical tests were followed for the detection and enumeration of bacterial isolates associated with these food samples. The investigation encompassed detection of total viable bacterial count (TVBC) and presumptive identification of other pathogenic bacteria from these samples. Higher counts of TVBC, coliform, Staphylococcus spp. and fungal load were recorded as 1.46×107 CFU/g (yogurt 1), 4.5×106 CFU/g (yogurt 1), 5.6×105 CFU/g (raw meat) and 2.9×103 CFU/g (sea fish), respectively. Fecal coliform was detected only in one out of ten samples. On the contrary Salmonella spp., Vibrio spp., Shigella spp. and Pseudomonas spp. were completely absent. The antibiogram study showed that all the isolates were sensitive against Kanamycin and Azithromycin. Better sensitivity was also observed against Gentamycin and Ciprofloxacin. Varying degree of antibiotic resistance was also detected against Cefixime, Amikacin and Neomycin. Our study emphasizes the need for continuous monitoring of the various categories of food samples for the safety of public health.

scholarly journals Microbiological analysis of ready to eat foods collected from different places of Dhaka city, Bangladesh

2019 ◽  
Vol 8 (1) ◽  
pp. 30-33
Author(s):  
Md Aftab Uddin
Keyword(s):  
Pathogenic Bacteria ◽  
Microbiological Quality ◽  
Bacterial Count ◽  
Food Samples ◽  
French Fries ◽  
Microbiological Analysis ◽  
Dhaka City ◽  
Biochemical Tests ◽  
Fish Fillet ◽  
Bacillus Spp

The current study was attempted to observe the microbiological quality of ready to eat foods collected from different places of Dhaka city, Bangladesh. Ten food samples that include five fried items-fried chicken, fish fillet, shrimp fries, French fries, potato chop and five sweet items-yoghurt, pudding, ice cream, mango drink, lemon drink were analyzed during the period from September 2017 to November 2017. Conventional cultural, microscopic and biochemical tests were followed for the detection and enumeration of bacterial isolates associated with these food samples. The investigation encompassed detection of total viable bacterial count (TVBC) and presumptive identification of other pathogenic bacteria from these samples. The higher counts of TVBC, Escherichia coli, Staphylococcus aureus and Bacillus spp. were recorded as 1.94×106 cfu/g (potato chop), 3.1×104 cfu/g (fried shrimp), 7.5×103 cfu/g (fried chicken) and 1.0 x 103 cfu/g (French fries) respectively. Based on the specifications by International Commission for Microbiological Specification for Foods (ICMSF), the level of contaminations was within acceptable microbiological limits except for potato chop. Stamford Journal of Microbiology, Vol.8(1) 2018: 30-33

scholarly journals Microbiological analysis of raw milk, pasteurized milk and yogurt samples collected from different areas of Dhaka city, Bangladesh

2015 ◽  
Vol 39 (1) ◽  
pp. 31-36 ◽  
Author(s):  
Tasmina Rahman ◽  
Tanzia Akon ◽  
Iftikharun Nessa Sheuli ◽  
Naima Hoque
Keyword(s):  
Listeria Monocytogenes ◽  
Bacterial Count ◽  
Raw Milk ◽  
Total Coliform ◽  
Microbiological Analysis ◽  
Dhaka City ◽  
Salmonella Spp ◽  
Pasteurized Milk ◽  
Milk Samples ◽  
Shigella Spp

A total of 300 different milk and yogurt samples were collected from Dhaka city and analyzed for total viable bacterial count (TVBC), total coliform count (TCC) and presence of several pathogens such as Escherichia coli, Salmonella spp., Shigella spp., Vibrio spp. and Listeria monocytogenes. Raw milk samples collected from Moghbazar area showed highest TVBC (4.2 × 106 cfu/ml) and samples from Mohammadpur area showed lowest count (3.5 × 103 cfu/ml). All the pasteurized milk samples showed TVBC of 1.9 × 102 to 2.8 × 103 cfu/ml, and TCC only in one sample Yogurt samples (open) collected from vendor showed TVBC of 9.1 × 103 to 8.2 × 107 cfu/ml, and TCC only in sample collected from Dhanmondi, Dhaka. The packed yogurt samples showed TVBC of 2.2 × 102 to 6.1 × 103 cfu/ml, and TCC only in one sample. All the milk and yogurt samples were found contaminated with E. coli and Shigella-like species. Listeria monocytogenes was not detected in any of the samples studied.Journal of Bangladesh Academy of Sciences, Vol. 39, No. 1, 31-36, 2015

scholarly journals Study of Microbiological Quality of Fresh Juices Vended in Dhaka City

2019 ◽  
Vol 36 (1) ◽  
pp. 49-53
Author(s):  
Kayseri Afroz ◽  
Tahmina Shammi ◽  
Md Shahidul Kabir
Keyword(s):  
Pathogenic Bacteria ◽  
Bacterial Load ◽  
Microbiological Quality ◽  
Antibiotic Sensitivity ◽  
Multi Drug Resistance ◽  
Dhaka City ◽  
Salmonella Spp ◽  
Street Vendors ◽  
Shigella Spp ◽  
Vibrio Spp

Fresh juices sold by the street vendors are rich in nutritional value and considered as popular drink in Dhaka city .They can be easily contaminated by pathogenic bacteria from unhygienic environment of food preparation area and serves as a potential source of food borne outbreaks. Freshly produced juices of papaya (n = 2), wood apple (n = 2), wood apple and papaya juice (1), lemon (n = 2), and sugarcane (n = 3) were examined for the total bacterial mload, coliforms, Staphylococcus spp., Pseudomonas spp., Vibrio spp., Salmonella spp., and Shigella spp. in this study. Total bacterial load in eight samples were within 0 to 107 cfu/ml, six samples exhibited the presence of staphylococci within the range of 0 to 105 cfu/ml. Total coliforms were detected in six samples which ranged from 104 to 105 cfu/ ml. Vibrio spp., Salmonella spp., and Shigella spp. were isolated by enrichment and selective plating method. Eight samples demonstrated the presence of Vibrio spp. and none of them were positive for Salmonella spp. and Shigella spp. Antibiotic sensitivity patterns were determined against eight isolates using Erythromycin (15μg), Amoxicillin (10 μg), Gentamicin (10 μg), Vancomycin (30 μg), Ciprofloxacin (5 μg), Rifampicin (5 μg), Azithromycin (15 μg), Neomycin (10 μg) and Cefexime (5 μg). Klebsiella sp. (n = 1), Vibrio sp. (n = 2), Enterobacter sp. (n = 1), and Proteus sp. (n = 1) showed multi drug resistance in this study. Contamination of street vended juices pose risks to consumers health and requires adequate attention of appropriate authority. Bangladesh J Microbiol, Volume 36 Number 1 June 2019, pp 49-53

scholarly journals Isolation and quantification of indicator and pathogenic microorganisms along with their drug resistance traits from bottled and jar water samples within Dhaka city, Bangladesh

2020 ◽  
Vol 9 (1) ◽  
pp. 12-14
Author(s):  
Md Aftab Uddin ◽  
Mst Aysha Siddiqua ◽  
Mst Sadia Ahmed
Keyword(s):  
Water Samples ◽  
Bacterial Count ◽  
Fecal Coliforms ◽  
Pathogenic Microorganisms ◽  
Dhaka City ◽  
Bacterial Isolates ◽  
Salmonella Spp ◽  
Potential Threat ◽  
Antibiotic Susceptibility Pattern ◽  
Shigella Spp

Commercial drinking water may serve as potential threat to public health if these items are contaminated with a number of pathogenic microorganisms due to faulty manufacturing process. Present study attempted to isolate and quantify the microorganisms from various jar and bottle water samples collected from various areas of Dhaka city. Antibiotic susceptibility pattern of suspected bacterial isolates were also determined in this study. Out of the eighteen samples studied, ten were jar water samples and eight were bottled water samples. The range of total viable bacterial count (TVBC) in these samples ranged from 102 to 105 cfu/ml. Specific pathogens such as, Salmonella spp., Shigella spp., Vibrio spp. and fecal coliforms could not be found in these samples. However coliforms could be detected in 10 samples. The antibiogram study showed that all Escherichia coli and Klebsiella spp. isolates found from these samples were sensitive against gentamicin (10 μg) and azithromycin (30 μg). Variable antibiotic resistance among these bacterial isolates was detected against cefotaxime (30 μg), streptomycin (10 μg) and erythromycin (15 μg). Stamford Journal of Microbiology, Vol.9(1) 2019: 12-14

scholarly journals Microbiological quality and somatic cells of in natura milk produced in Alagoas State, Brazil

2020 ◽  
Vol 9 (9) ◽  
pp. e412997379
Author(s):  
Bruno Fernando Oliveira Araújo ◽  
Sybelle Geórgia Mesquita da Silva ◽  
João Manoel da Silva ◽  
Cícero Cerqueira Cavalcanti Neto ◽  
Paula Cibelly Vilela da Silva ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Economic Value ◽  
Somatic Cells ◽  
Microbiological Quality ◽  
Raw Milk ◽  
Microbiological Analysis ◽  
Salmonella Spp ◽  
Psychrotrophic Bacteria ◽  
Physical Chemical ◽  
Significant Difference

Raw milk is a food with great consumption and economic value in Brazil. However, is susceptible of contamination by pathogenic bacteria. The aimed of this study was to evaluate the quality of in natura milk based on microbiological in three dairy farms, somatic cells counting (SCC), bacterial counting and his physical-chemical composition. Were made the following microbiological analysis: counting of mesophilic and psychrotrophic bacteria, coliforms at 30 ºC, coliforms at 45 ºC, Staphylococcus spp., Listeria spp., and SCC. The physical-chemical analysis was fat, protein, lactose, total solids, urea, and casein. There was no evidence of Salmonella spp. and Escherichia coli were identified in any samples.  In accordance to the microbiological standards established by Normative Instruction 76 only coliforms 30 ºC and 45 ºC counts were above the standards. There was a significant difference (p≤0.05) between the three farms studied regarding most microbiological aspects. Also, was observed difference (p≤0.05) for most of physical-chemical aspects. Overall, the milk produced in the regions of Alagoas State fails to meet just a constant criterion in the current legislation.

scholarly journals Microbiological quality assessment for drug resistant pathogenic microorganisms from the fresh vended fruit juices

2017 ◽  
Vol 6 (1) ◽  
pp. 7-10 ◽  
Author(s):  
Nurunnahar Akter Lucky ◽  
Ifra Tun Nur ◽  
Tasnia Ahmed
Keyword(s):  
Pathogenic Bacteria ◽  
Microbiological Quality ◽  
Fruit Juices ◽  
Drug Resistant ◽  
Pathogenic Microorganisms ◽  
Salmonella Spp ◽  
Juice Processing ◽  
Nutritive Values ◽  
Shigella Spp ◽  
Vibrio Spp

Fruit juices are very popular due to their freshness and taste. These juices contain all the nutritive values those were present in whole fruits. Fresh juices are healthful drink for people of all ages. But the condition is quite opposite if they become contaminated with pathogenic bacteria during the juice processing. Because of the nutritive properties juice can give enough opportunities to the microorganisms to survive and multiply. In our current study we included eight types of fresh juices to study the microbial load. All the samples were heavily contaminated. Total bacterial and fungal count was found to be up to 3.0×107 cfu/ml and 2.6×106 cfu/ml, respectively. Salmonella spp. and Shigella spp. was present only in one sample. Staphylococcus aureus and Pseudomonas spp. were the most prominent in all of the eight samples. Vibrio spp. was found in six samples ranging from 2.0×102 cfu/ml to 2.9×104 cfu/ml. All the pathogenic isolates showed drug resistance towards the most commonly prescribed antibiotics indicating the risk of difficulty in eradicating diseases.Stamford Journal of Microbiology, Vol.6(1) 2016: 7-10

scholarly journals Microbiological Quality Assessment of Foods collected from Different Hospitals within Dhaka City

1970 ◽  
Vol 1 (1) ◽  
pp. 31-36 ◽  
Author(s):  
Fazle Alam Rabbi ◽  
Fazle Rabbi ◽  
TA Runun ◽  
Khadiza Zaman ◽  
M Majibur Rahman ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Microbiological Quality ◽  
Multidrug Resistant ◽  
Total Coliform ◽  
Food Samples ◽  
Biochemical Tests ◽  
Salmonella Spp ◽  
Coliform Count ◽  
Antibiotic Susceptibility Test ◽  
Microbiological Criteria

Various types of cooked food samples were collected from six different hospitals within the city of Dhaka and they were analyzed for the presence (if any) of total aerobic count (TAC), total coliform count (TCC) and total staphylococcal count (TSA) in order to determine the levels of contamination and to relate these findings to the hygiene practice of the food handlers. According to Gulf standard for microbiological criteria for foodstuff, all of the food samples exceeded the acceptable total aerobic count limit of 5 x 105 cfu/g while 4 out of 6 samples exceeded coliform count limit of 1x102 cfu/g. The total coliform counts were found to be the highest in the fish (1.6 x 107 cfu/g) and egg (2.2 x 106 cfu/g) curry samples of hospital 1 and hospital 4, respectively and were the lowest in the fish curry (2 x 103 cfu/g) of hospital 4. Staphylococcus aureus was found in all of the food samples with the highest occurrence (too numerous to count) in Dal and Rice samples from hospitals 3 and 6, respectively. On Xylose Lysine Deoxycholate (XLD) agar, no black centered colony but many colorless colonies were found which primarily indicated the absence of Salmonella spp. in those samples. In order to identify pathogenic microorganisms from food samples, a series of conventional biochemical tests were performed with 23 randomly selected isolates from MacConkey, XLD, MSA agar plates. The isolates were presumptively identified as Escherichia coli, Staphylococcus aureus, Shigella spp. and Pseudomonas spp. etc. The antibiotic susceptibility test was performed with eleven selected isolates using six commonly prescribed antibiotics (ampicillin, tetracyclines, ciprofloxacin, vancomycin, gentamicin and azithromycin). The results showed that six isolates were resistant to vancomycin, two isolates were multidrug resistant and one isolate was intermediately resistant to azithromycin. All the isolates were found to be sensitive to ciprofloxacin, and gentamicin. Based on the data, it can be suggested that adequate hygiene practices are required after cooking the foods and before serving them as they reconsider.DOI: http://dx.doi.org/10.3329/sjm.v1i1.9100  Stamford Journal of Microbiology, Vol.1(1), July 2011, p.31-36  

scholarly journals Microbiological Study on Supply Water and Treated Water in Dhaka City

1970 ◽  
Vol 1 (1) ◽  
pp. 42-45 ◽  
Author(s):  
Mrityunjoy Acharjee ◽  
Farjana Rahman ◽  
Sadia Afrin Beauty ◽  
Farahnaaz Feroz ◽  
M Majibur Rahman ◽  
...  
Keyword(s):  
Water Samples ◽  
Total Coliform ◽  
Faecal Coliform ◽  
Microbiological Analysis ◽  
Dhaka City ◽  
Sample Collection ◽  
Biochemical Tests ◽  
Salmonella Spp ◽  
Treated Water ◽  
Two Samples

Water borne diseases are the major cause of morbidity and mortality in developing countries like Bangladesh, where disease outbreaks occur due to unsafe drinking water, inadequate sanitation and poor hygienic practices. The objective of this study was to evaluate the quality of water used for drinking as well as household purposes in Dhaka city, Bangladesh. Eighteen water samples were collected from different rivers and canals. Among those, two samples were treated with chlorine. Physico-chemical parameters (pH, salinity, turbidity, conductivity and dissolved oxygen) were monitored during the time of sample collection using portable meters. For microbiological analysis, water samples were collected in sterile screw capped bottle. All Experiments were carried out within 24 hours after collecting the samples. All the water sources were found to be contaminated with total coliform, faecal coliform, Escherichia coli, Klebsiella spp., Salmonella spp., Shigella spp., Vibrio cholerae, Aeromonas spp. and Fungi except treated water samples. Several biochemical tests were performed for confirmation of suspected organisms. According to these results, huge amount of total coliform and faecal coliform were observed in every sample and these amount were very much similar for other organisms. Treated water samples were found to be microbiologically accepted than untreated water samples. DOI: http://dx.doi.org/10.3329/sjm.v1i1.9132    Stamford Journal of Microbiology, Vol.1(1), July 2011, p.42-45

scholarly journals Microbiological quality assessment and acceptance of dairy products in Dhaka city

2017 ◽  
Vol 1 (1) ◽  
pp. 58-64
Author(s):  
Md Nur Hossain ◽  
Sanjida Humayun ◽  
Md Zahurul Haque ◽  
Monzur Morshed Ahmed
Keyword(s):  
Public Health ◽  
Dairy Products ◽  
Health Hazard ◽  
Microbiological Quality ◽  
Dhaka City ◽  
Salmonella Spp ◽  
Street Vendor ◽  
Shigella Spp ◽  
A Minor

This investigation was carried out to evaluate the microbiological quality of the processed dairy products including borhani, matha/labang, sweet & sour yoghurt. Dairy products are consumed as desert and popular enough among the people. 50 of the different dairy samples were collected from street vendor and also from some branded shop in Dhaka city. The microbiological quality of the samples were analyzed and monitored according to criteria in European Commission Recommendations 2004/24/EC and 2005/175/EC, BSTI and USPHS. All the dairy products had high microbial load ranged 5.90×105cfu/ml to 8.97×109 cfu/ml. Coliforms were found up to 109 dilutions in milk based drink products Borhani and Labang considered a serious threat to the public health. The mold contamination was much lower in Borhani and Labang compared to yoghurt 7.86×108cfu/ml. Approximately 17% dairy products were contaminated by Salmonella spp. Majority of the dairy products (25.537%,) such as Yogurt, Borhani and Labang were contaminated by Staphylococcus spp. The presence of Listeria monocytogenes found in Yogurt, 7 out of 21 (21.515%) samples were contaminated. A minor number of milk based products were corrupted by Shigella spp. These results emphasize applying and maintaining good hygiene practices throughout the processing chain to prevent contamination and bacterial growth. It was concluded that the presence of some pathogens in milk based products with toxigenicity of some strains pose a health hazard to consumers. Thus, good hygienic practices, good manufacturing practices, HACCP with all other hygienic practices should be applied during processing and distribution for public health safety. Asian Australas. J. Food Saf. Secur. 2017, 1 (1), 58-64

Microbiological quality of a waste stabilization pond effluent used for restricted irrigation in Valle Del Cauca, Colombia

2002 ◽  
Vol 45 (1) ◽  
pp. 139-143 ◽  
Author(s):  
C.A. Madera ◽  
M.R. Peña ◽  
D.D. Mara
Keyword(s):  
Pathogenic Bacteria ◽  
Microbiological Quality ◽  
Salmonella Spp ◽  
Helminth Eggs ◽  
Waste Stabilization ◽  
Shigella Spp ◽  
Field Workers ◽  
Valle Del Cauca ◽  
Final Effluent

This paper discusses the applicability of effluent reuse in agriculture after treatment in a series of anaerobic, facultative and maturation ponds. The WSP system is located in Ginebra municipality, a small town in southwest Colombia. The total HRT is 12 days. Several samples of the final effluent were taken over a 55 day period and were analysed for E.coli, Streptococcus spp. and helminth eggs. Some additional grab samples were taken to determine the presence of pathogenic bacteria such as Salmonella spp. and Shigella spp. The results showed that the system was able to remove 4 log units of E.coli, 1 log unit of Streptococcus spp. and 100% of helminth eggs. Meanwhile, Salmonella spp. were detected in the effluent of the facultative pond whilst Shigella spp. were not detected in any sample. The main species of helminth eggs encountered were Taenia spp., Ascaris spp., Trichuris spp., Hymenolepis nana, H. diminuta and Enterobius vermicularis. Removal efficiencies were satisfactory despite the relatively short HRT. Nevertheless, WHO guidelines were slightly surpassed in the case of E.coli for unrestricted irrigation. The helminth egg value was always below the maximum WHO limit. Hence, this effluent can be safely used for restricted irrigation provided that field workers are protected from direct contact with wastewater given the presence of Salmonella spp. in the facultative pond effluent.

Sign in / Sign up

Export Citation Format

Share Document