scholarly journals Efficacy study of bio-typhoid® vaccine against fowl typhoid in backyard layer chicken

1970 ◽  
Vol 7 (2) ◽  
pp. 295-299
Author(s):  
MZ Uddin ◽  
MSR Khan ◽  
F Begum ◽  
MA Mannan
Keyword(s):  
Antibody Titre ◽  
Virulent Strain ◽  
Primary Vaccination ◽  
Control Group ◽  
Fowl Typhoid ◽  
Layer Chicken ◽  
Unvaccinated Control ◽  
Antibody Titres ◽  
Group A ◽  
Group B

The study was performed with a view to isolate and identify a virulent strain of S. gallinarum and determine the purity, safety and efficacy of BIO-TYPHOID® vaccine. A total of 40 backyard layer chicken were used for this study where Group A was used for experimental vaccination, Group B kept as control and Group C was used for calculating virulent S. gallinarum challenge dose. Primary and secondary vaccination was carried out at 40 days and 110 days of age, respectively. Blood samples were collected to obtain the sera after vaccination from both vaccinated and unvaccinated control group and antibody titres were determined by Microplate agglutination test. The antibody titre increased in primary vaccination up to days 56 days post vaccination (DPV) and then decreased gradually. The highest antibody titre (Mean ± SE) 384.00 ± 42.67 was obtained at 91 DPV (21 days later of secondary vaccination) and maintained up to 98 DPV. Safety test was done by inoculating mice and purity test of the vaccine was done by inoculating on to Blood Agar media. The efficacy of BIO-TYPHOID® vaccine was recorded as 90% which was determined by challenge infection with 0.1 ml of 5x105 CFU virulent S. gallinarum. Results of this study revealed successful protections by BIO-TYPHOID® vaccine. Keywords: BIO-TYPHOID® vaccine; Chicken; Efficacy; Fowl Typhoid DOI: 10.3329/jbau.v7i2.4737 J. Bangladesh Agril. Univ. 7(2): 295-299, 2009

scholarly journals Effect of vitamins, minerals and probiotics on production of antibody and live weight gain following vaccination with BCRDV in broiler birds

1970 ◽  
Vol 6 (1) ◽  
pp. 31-36 ◽  
Author(s):  
P Monoura ◽  
M Rahman ◽  
MFR Khan ◽  
MB Rahman ◽  
MM Rahman
Keyword(s):  
Antibody Titre ◽  
Serum Antibody ◽  
Live Weight ◽  
Poultry Feed ◽  
Control Group ◽  
Broiler Chicks ◽  
Antibody Titres ◽  
Group A ◽  
Group B ◽  
Group D

The effect of vitamins, minerals and probiotics supplementation to the balanced ration of the broiler chicks on the immune response and growth performance after vaccination with BCRDV was evaluated. For this 50 broiler birds were divided into five equal groups such as A, B, C, D and E, and five types of separate treatments with normal poultry feed were provided to the indivisual experimental groups. All the birds were vaccinated with BCRDV primarily at 3 days of age and boostered at 17 days of age. The birds of group A, B, C and D were supplemented with only probiotics, mixture of probiotics and minerals, mixture of probiotics, minerals and vitamins, respectively. The birds of group E were kept as vaccinated control. Blood samples were collected at the age of 9, 12, 16, 23, 26 and 30 days sequencially. The serum antibody titres were measured by HI test and ELISA. The highest value of HI antibody titre was 384±242.65 (Mean ±SD) in group B at 23 days of age. The lowest value of HI at 23 days was 56±32.86 (Mean ±SD) obtained in group C. The ELISA antibody titre was also highest (4675.12±485.72) at 23 days in group B and obviously the lowest (2169.38±724.45) in control group E. In case of group C, the ELISA antibody titre was 3105.73±877.57 at 23 days but then the titre decreased gradually compared to control group. The highest body weight (1420±90.82) was recorded at 30 days of age in group D and the lowest value (1188±44.38) was in group B. Key words: Probiotics, BCRDV, broiler birds DOI = 10.3329/bjvm.v6i1.1336 Bangl. J. Vet. Med. (2008). 6 (1): 31-36

scholarly journals Comparative immunogenicity of fowl cholera vaccine in Jinding ducks

2014 ◽  
Vol 30 (2) ◽  
pp. 41-45 ◽  
Author(s):  
S Sultana ◽  
S Saha ◽  
MM Amin
Keyword(s):  
Pasteurella Multocida ◽  
Booster Vaccination ◽  
Primary Vaccination ◽  
Challenge Infection ◽  
Fowl Cholera ◽  
Significant Difference ◽  
Unvaccinated Control ◽  
Antibody Titres ◽  
Group A ◽  
Group B

This study compared the immunogenicity of alum-precipitated formalin-killed fowl cholera vaccines (BAU-FCV and LRI-FCV) in Jinding ducks. The ducks were divided into three groups (A = 14, B = 14, C = 12). Group A was inoculated with BAU- FCV 0.5 mL and group B with LRI- FCV 1.0 mL intramuscularly (im) at the age of six weeks and group C served as unvaccinated control. Booster vaccination was administered similarly at 11 weeks of age in groups A and B. Challenge infection was given to all birds two weeks after booster vaccination. Passive Haemagglutination Assay (PHA) antibody titres in group A were 59.4 ± 4.6 21 days after primary vaccination, 137.1 ± 21.8 15 days after booster vaccination, 100.6 ± 12.9 21 days after booster vaccination, and 256.0 ± 48.4 15 days after challenge. In group B, titres were 50.3 ± 6.5, 118.9 ± 9.1, 91.4 ± 12.9, 237.7 ± 51.7, respectively, whereas titres in group C remained at ?4.0 ± 0.0. The antibody titres were insignificant when compared between pre-vaccination and 21 days after primary vaccination in both vaccinated groups (A and B). PHA antibody titres of groups A were significantly (P < 0.0001) increased at 15 days after booster and in case of group B the antibody titres were insignificant. At 15 days after challenge the antibody titres were highly significant in both groups (A and B). There was no significant difference between the two vaccinated groups. Following challenge infection with virulent Pasteurella multocida 88.9% of birds vaccinated with BAU-FCV, and 77.8% of birds vaccinated with LRI-FCV survived, while all unvaccinated birds died. Both vaccines were safe and effective. DOI: http://dx.doi.org/10.3329/bvet.v30i2.18253 Bangl. vet. 2013. Vol. 30, No. 2, 41-45

scholarly journals COMPARATIVE IMMUNOGENICITY OF BIOTYPHOID® AND DLS PREPARED FOWL TYPHOID VACCINE IN LAYER CHICKENS

1970 ◽  
Vol 8 (1) ◽  
pp. 01-04
Author(s):  
J Ferdous ◽  
MSR Khan ◽  
F Begum ◽  
J Hassan ◽  
B Sarker
Keyword(s):  
Antibody Titre ◽  
Typhoid Vaccine ◽  
Animal Science ◽  
Post Vaccination ◽  
Fowl Typhoid ◽  
Unvaccinated Control ◽  
Antibody Titres ◽  
Group A ◽  
Layer Chickens

The experiment was conducted to investigate the immunogenicity of DLS prepared Fowl typhoid vaccine in comparison to commercially available BIO-TYPHOID® (Imported by Advance Animal Science Co. Ltd., Bangladesh) in layer chickens. To compare the immunogenicity of these two vaccines a total of 30 chickens were divided into three groups (group A, B and C) each including 10 layer chickens of Fayoumi breed. Chickens of group A and B were vaccinated with DLS prepared fowl typhoid vaccine and BIO-TYPHOID® respectively following usual schedule of vaccination (i.e. first dose at 42 days of age and second dose at 72 and 112 days for DLS prepared fowl typhoid vaccine and BIO-TYPHOID® respectively) with a dose of 0.5 ml at each occasion through SC route. Birds of group C were kept as unvaccinated control. Blood samples were collected to obtain sera from each chicken at every 7 days interval up to 105 days post vaccination for determination of antibody titre following primary and secondary vaccination using Microplate Agglutination test. Highest mean antibody titres obtained from Group A and B which were 460.80 and 409.60 respectively. Among the chickens vaccinated the highest mean antibody titre of 460.80 was obtained at 56 days post vaccination from chickens in group A vaccinated with DLS prepared Fowl typhoid vaccine. Results of this study revealed that DLS prepared fowl typhoid vaccine induced higher antibody production than BIO-TYPHOID®. DOI = 10.3329/bjvm.v8i1.7394 Bangl. J. Vet. Med. (2010). 8(1): 01-04

scholarly journals Field Investigation on the Efficacy of Salmonella Vaccine Prepared at Bangladesh Agricultural University

2015 ◽  
Vol 13 (1) ◽  
pp. 5-9
Author(s):  
MAS Bag ◽  
MM Amin ◽  
MB Rahman ◽  
YA Arafat ◽  
M Salim ◽  
...  
Keyword(s):  
Research Work ◽  
Booster Vaccination ◽  
Primary Vaccination ◽  
Field Investigation ◽  
Unvaccinated Control ◽  
Antibody Titres ◽  
Group A ◽  
The Mean ◽  
Group B ◽  
On Farm

The research work was performed to investigate the immunogenicity of Salmonella vaccine produced at LPVRPC, BAU Mymensingh. The vaccination was performed at the Phenix Hatchery Ltd. Gazipur in Hy-sex brown and HY-sex white chicken designated as group A and group B respectively. Group A and B were subdivided into A1, A2, A3, B1, B2 and B3 groups containing eight birds each. Group C was maintained as unvaccinated control. Birds were immunized following schedule of the LPVRPC. Each bird was vaccinated SC at six weeks of age followed by a subsequent booster dose after 45 days. After four weeks of primary vaccination the mean PHA antibody titres were 96.00±34.21 in group A1 and 96.00±34.21 in B1 group. Prebooster vaccination the mean PHA antibody titres were 88.00±33.12 in group A2 and 88.00±33.12 in B2 group. At four weeks of booster vaccination the mean PHA antibody titres were 104.00±33.12 in group A3 and 104.00±33.12 in B3 group. The mean ± PHA antibody titre in chickens of group C was ? 4.0±0.00. Salmonella vaccine prepared at (LPVRPC) Department of Microbiology and Hygiene, BAU induces satisfactory level of antibody in chickens determined by PHA test conducted in an on-farm study of layer chickens.DOI: http://dx.doi.org/10.3329/bjvm.v13i1.23705Bangl. J. Vet. Med. (2015). 13 (1): 5-9

scholarly journals Evaluation of Salmonella Gallinarum ghost formulated with Montanide™ ISA 70 VG adjuvant as a vaccine against fowl typhoid

2015 ◽  
Vol 63 (4) ◽  
pp. 401-412 ◽  
Author(s):  
Chetan V. Jawale ◽  
Nithiphonh Somsanith ◽  
Seong Kug Eo ◽  
Sang-Youel Park ◽  
John Hwa Lee
Keyword(s):  
Virulent Strain ◽  
Survival Rates ◽  
Control Group ◽  
Mrna Levels ◽  
Salmonella Gallinarum ◽  
B Subunit ◽  
Fowl Typhoid ◽  
Group A ◽  
Group B ◽  
Th1 Cytokines

Escherichia coli heat-labile enterotoxin B subunit (LTB) protein is a potent adjuvant. Salmonella Gallinarum ghosts carrying LTB (S. Gallinarum-LTB ghosts) were genetically constructed using a plasmid, pJHL187-LTB, designed for the co-expression of the LTB and E lysis proteins. This study evaluates the immunopotentiating effects of Montanide™ ISA 70 VG on S. Gallinarum-LTB ghost vaccination against fowl typhoid. Five-week-old layer chickens were injected intramuscularly with sterile PBS (non-immunised control, Group A), S. Gallinarum-LTB ghost (Group B) or S. Gallinarum-LTB ghost emulsified with Montanide™ ISA 70 VG adjuvant (Group C). Chickens from both Groups B and C showed significant induction of antigen-specific systemic IgG response compared to controls; in addition, Group C showed enhanced induction of systemic IgG response compared to Group B. We observed significant induction of antigen-specific lymphocyte proliferative response and increased mRNA levels of Th1 cytokines (IFN-γ and IL2) in both Groups B and C. Furthermore, in the challenge experiment with a virulent strain of S. Gallinarum, Group C showed higher survival rates compared with other groups. These results indicate that vaccination with the S. Gallinarum-LTB ghost in combination with Montanide™ ISA 70 VG may enhance the protective immunity against fowl typhoid.

scholarly journals Antibody responses in buffalos immunized with Clostridium perfringens beta and epsilon toxoids

2001 ◽  
Vol 46 (No. 9–10) ◽  
pp. 241-243 ◽  
Author(s):  
S. Rahman M ◽  
K. Baek B ◽  
T. Hong S ◽  
H. Lee J
Keyword(s):  
Antibody Titre ◽  
Clostridium Perfringens ◽  
Neutralizing Antibody ◽  
Antibody Responses ◽  
Control Group ◽  
Humoral Immune ◽  
Humoral Immune Responses ◽  
Antibody Titres ◽  
Group A ◽  
And Control

The antibody responses to toxoids were measured to investigate whether&nbsp;Clostridium perfringens&nbsp;beta and epsilon toxoids induced protective humoral immune responses in buffalos. Total of 24 buffalos were divided into 4 groups (n&nbsp;= 6), beta toxoid, epsilon toxoid, combination and control groups. These buffalo groups were administered each of the designated toxoids. Immunizations in the beta and epsilon toxoid groups induced strong antibody responses. The neutralizing antibody titres from the beta and epsilon toxoid groups were equally log101.2 on day 21 after inoculation whereas there was no antibody titre detected from the control group. A statistically significant (P&nbsp;&lt; 0.01) increase in antibody titre was observed from day 0 to day 14 and 21 after inoculation. The antibody production did not vary significantly due to day of inoculation and toxoid interactions.

scholarly journals The Efficacy of Ranikhet Disease Vaccines Produced by Livestock Research Institute of Bangladesh

2013 ◽  
Vol 1 (1) ◽  
pp. 9-13 ◽  
Author(s):  
Sreebas Chandra Sarkar ◽  
Sukumar Saha ◽  
Md Mansurul Amin ◽  
Md Golzar Hossain
Keyword(s):  
Field Isolate ◽  
Challenge Infection ◽  
Control Group ◽  
Passive Protection ◽  
Field Virus ◽  
Unvaccinated Control ◽  
Antibody Titers ◽  
Group A ◽  
Group B ◽  
Research Institute

The study was conducted to investigate the efficacy of Baby chick Ranikhet Disease Vaccine (BCRDV) and Ranikhet Disease Vaccine (RDV) produced by the Livestock Research Institute (LRI), Mohakhali, Dhaka. For this experiment, 100 day-old-chick was purchased from Phinex Hatchery Ltd., Gazipur. The chicks (n=100) were divided into two groups. In group A (n=50), vaccination was performed twice with BCRDV at 2 and 21 days of age through intraocular route (i/o) followed by once with RDV at 60-day of age through intramuscular (i/m) route. Group B (n=50) was kept as unvaccinated control. The immunogenicity of the vaccine was evaluated by measuring the serum HI antibody titers at 1-, 20-, 36-, and 76-day of age, while the vaccine efficacy was examined by a challenge infection experiment with a velogenic field isolate of NDV as well as passive protection test. It was observed that the maternal antibody titers of the unvaccinated control group B gradually declined from day 1 to day 76 of age. Conversely, after primary and secondary vaccination with BCRDV, the levels of serum HI titer slightly increased in vaccinated group A compared with those in control group B. Finally administration of RDV resulted in a sharp increase in HI titer, leading to protection from challenge infection with virulent field virus as well as passive protection test. These results clearly demonstrated that a prime-booster immunization with BCRDV and RDV, both produced by LRI, is effective to protect chicken against Newcastle disease (ND).DOI: http://dx.doi.org/10.3329/mh.v1i1.13706 Microbes and Health Vol.1(1) June 2012 pp.9-13

scholarly journals THE POTENTIAL OF ADJUVANT AGAINST PRODUCTION OF ANTISTREPTOCOCCAL IMMUNOGLOBULIN Y (IGY) IN AQUACULTURE

2020 ◽  
Vol 14 (3) ◽  
Author(s):  
Rifky Rizkiantino ◽  
I Wayan Teguh Wibawan ◽  
Fachriyan Hasmi Pasaribu ◽  
Retno Damajanti Soejoedono ◽  
Okti Nadia Poetri ◽  
...  
Keyword(s):  
Treatment Group ◽  
Enterococcus Faecalis ◽  
Egg Yolk ◽  
Control Group ◽  
Fast Time ◽  
Immunoglobulin Y ◽  
Layer Chicken ◽  
Group A ◽  
Group B ◽  
And Control

This study was conducted to explore the potential of adjuvant for the production of immunoglobulin Y (IgY) as antistreptococcosis in layer chicken with mass production orientation. Enterococcus faecalis which causes streptococcosis in the red tilapia was selected as a candidateantigen. The production of immunoglobulin Y (IgY) was carried out on Isa Brown layer chickens and aged around 20 weeks. Furthermore, thechickens were grouped into four groups (A, B, C, and D groups), each consisting of three chickens based on the type of adjuvant, while twochickens were used as a control group. Each group was treated by giving MONTANIDE™ ISA 71R VG adjuvant (A), Freund's adjuvant (B), aluminum potassium sulphate adjuvant (KAl(SO4)2∙12H2O) concentration of 50 ppm in pH 7 (C), and only antigens without adjuvant (D). Chickens were kept for 35 days and each week was checked for presence the IgY antigen in the serum and egg yolk. Booster was conducted on 14th and 28th days of maintenance. The results showed that IgY in treatment group A was detected on day 28 in the serum and day 35 in the yolk. Whereas the treatment group B could be detected on day 35 in the serum. However, the IgY was not detected in the serum and yolk in C, D, and control groups until the end of the maintenance. Based on the results, it can be concluded that the appearance of IgY in serum and yolk in a relatively fast time is obtained in the combination of Enterococcus faecalis antigen with the emulsion of water-in-oil adjuvant (SEPPICMONTANIDE™ ISA 71R VG) compared to the other types of adjuvant that use in this study.

scholarly journals Immunogenicity of fowl typhoid vaccine in layer chickens

1970 ◽  
Vol 7 (1) ◽  
pp. 99-102 ◽  
Author(s):  
J Ferdous ◽  
MSR Khan ◽  
F Begum ◽  
J Hassan
Keyword(s):  
Antibody Titre ◽  
Agglutination Test ◽  
Typhoid Vaccine ◽  
Post Vaccination ◽  
Blood Samples ◽  
Fowl Typhoid ◽  
Layer Chicken ◽  
Group A ◽  
Layer Chickens

The immunogenicity of available fowl typhoid vaccine was studied in layer chickens following usual and suggested schedule of vaccination. A total of 30 chickens were divided into three groups; group A (vaccinated following usual schedule of vaccination), B (vaccinated following suggested schedule of vaccination) and C (as control), each containing 10 layer chickens of Fayoumi breed. Chickens of group A and B were vaccinated with available fowl typhoid vaccine produced by the Governments laboratory at a dose rate of 0.5ml at each occasion through SC route, where 1st dose was administered at 42 days and 35 days of age, respectively, and 2nd dose at 72 days and 65 days of age, respectively. Blood samples were collected to obtain sera from each chicken at every 7 days interval up to 105 days post vaccination for determination of antibody titre following primary and secondary vaccination using microplate agglutination test. The highest mean antibody titre obtained from Group A was 460.8 than that of B was 435.2, respectively. Among the two groups, the highest mean antibody titre of 460.8 was obtained at 56 days post vaccination in group A following usual schedule of vaccination. It revealed that government supplied fowl typhoid vaccine induced higher level of antibody production in both usual and suggested schedule of vaccination. Keywords: Immunogenicity; Fowl Typhoid; Vaccine; Layer chicken; Suggested; Usual DOI: 10.3329/jbau.v7i1.4970 J. Bangladesh Agril. Univ. 7(1): 99-102, 2009

Comparative Study on Production of Antibody in Broilers Vaccinated with Four Lyophilized Newcastle Disease Vaccine

1970 ◽  
Vol 27 (1) ◽  
pp. 1-5
Author(s):  
S Kamrunnahar ◽  
MM Alim ◽  
AHM Taslima ◽  
MA Islam ◽  
NC Paul
Keyword(s):  
Newcastle Disease ◽  
Control Group ◽  
Comparative Efficacy ◽  
Unvaccinated Control ◽  
Antibody Titers ◽  
Group A ◽  
The Mean ◽  
Group B ◽  
Group D ◽  
Newcastle Disease Vaccine

A comparative efficacy of four lyophilized Newcastle disease vaccine, namely, Medivac ND-LaSota®, BCRDV®, Izovac B1 Hitchner® and Cevac Vitapest-L® in regard to the production of Himagglutination Inhibition (HI)- antibody was accomplished. For this, a total of 75 chicks were equally divided into five experimental groups such as, A, B, C, D and E. The HI antibody titers of group A vaccinated on 5 and 21 days of age with Medivac NDLaSota ® fluctuated among the Mean ± SD of 89.60 ± 33.05, 102.40 ± 33.05 and 192 ± 67.46 at 15 (10 DPV), 19 (14 DPV) and 31 (26 DPV) days of age respectively. As regards, group B administered with BCRDV® on the same days, the HI titers varied with the Mean ± SD of 83.20 ± 30.91, 102.40 ± 33.04 and 204.80 ± 66.09 at 15 (10 DPV), 19 (14 DPV) and 31 (26 DPV) days of age respectively. When considered the HI antibody titers of group C inoculated with Izovac B1 Hitchner® on days 5 and 21 of age, HI titer exhibited the Mean ± SD of 80.00 ± 43.33, 96.00 ± 33.73 and 192.00 ± 67.46 at 15 (10 DPV), 19 (14 DPV) and 31 (26 DPV) days of age respectively. Elucidation of HI antibody titers of group D birds receiving Cevac Vitapest-L® on same days were recorded to be of Mean ± SD of 96.00 ± 33.73, 115.20 ± 26.98 and 320.00 ± 173.31 at 15 (10 DPV), 19 (14 DPV) and 31 (26 DPV) days of age respectively. In birds of unvaccinated control group E, HI titers were found to be ranged from 32- 64, 16-32, 8-16, 4-8 and 2-4 with Mean ± SD of 48.00 ± 16.87, 24.00 ± 8.43, 11.20 ± 4.13, 5.20 ± 1.93 and 3.40 ± 0.97 on 3, 15, 17, 19 and 31 days of age respectively. Thus, it was found that birds of group A, B, C and D vaccinated with Medivac ND-LaSota®, BCRDV®, Izovac B1 Hitchner® and Cevac Vitapest-L® induced slightly higher level of HI antibody titers than that of BCRDV. Maternally derived antibody (MDA) persisted to a minimal level until the age of chicks of day 17 and later on MDA declined. Keywords: Vaccine; Antibody Titer; Newcastle Disease VirusDOI: http://dx.doi.org/10.3329/bjm.v27i1.9159 BJM 2010; 27(1): 1-5

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