scholarly journals Effectiveness of bioactive compounds in amending the soil infested with soil-borne Ralstonia solanacearum of potato

2022 ◽  
Vol 7 (1) ◽  
pp. 43-50
Author(s):  
Z Karim ◽  
MS Hossain
Keyword(s):  
Organic Matter ◽  
Bioactive Compounds ◽  
Ralstonia Solanacearum ◽  
Cow Dung ◽  
Infested Soil ◽  
Significant Difference ◽  
Disease Reduction ◽  
Cent Disease ◽  
Significant Disease ◽  
Amended Soil

Three natural bioactive compounds, viz. cow dung, bee propolis and turmeric powder were applied to amend the Ralstonia solanacearum infested soil of potato. Sun dried cow dung @ 30 t/ha, aqueous extraction of turmeric powder @ 30 kg/ha and propolis @ 5 lit/ha were applied to the inoculated soil by mixing with field soil in SAU (Dhaka) and BARI (Gazipur). Data on pH, organic matter, total cfu/ml and avirulent cfu/ml of the treatment applied soil and per cent disease index (PDI) of bacterial wilt of potato were recorded. Significant difference in soil pH and per cent organic matter (OM) occurred in all the amended soil as compared to control. A decreased total cfu/ml of R. solanacearum occurred in all the treated soil, but propolis (8.2 X 107) and cow dung (1.1 X 108) showed the lowest count. However, in increasing the avirulent count of R. solanacearum, turmeric powder (1.8 X 109) showed the highest count compared to other amended soil. Significant disease reduction over control was also observed in all the treatments, but propolis (45.65%) and turmeric powder (43.48%) showed the best results in wilt disease reduction. J. Biodivers. Conserv. Bioresour. Manag. 2021, 7(1): 43-50

scholarly journals Management of Ralstonia solanacearum (potato wilt disease) virulence by using bioactive compounds

2021 ◽  
Vol 6 (1) ◽  
pp. 65-76
Author(s):  
Z Karim ◽  
MSM Chowdhury ◽  
MS Hossain
Keyword(s):  
Bioactive Compounds ◽  
Ralstonia Solanacearum ◽  
Management Practices ◽  
Severity Index ◽  
Diffusion Method ◽  
Cow Dung ◽  
Tetrazolium Chloride ◽  
Inhibition Zones

Potato is an important vegetable crop of Bangladesh which is facing challenges worldwide due to a quarantine pathogen, Ralstonia solanacearum. It is a very successful bacterial pathogen against most of the traditional management practices. Suspension of ten bioactive compounds viz. propolis, honey, turmeric powder+oil, turmeric powder, magnesium chloride, boiled rice fluid, boiled rice fluid+iodine, sun dried cow dung powder, honey+iodine and sodium bicarbonate were evaluated and compared with control (sterile water), commercial bactericide (Krosin AG) and farmers practice (stable bleaching powder). In vitro assessment was done by comparing the inhibition zones produced on TZC (tetrazolium chloride) solid medium in disc diffusion method. All of those compounds produced larger inhibition zones as compared to control which indicated the effectiveness of the test compounds against the bacteria. To screen out the performances of those compounds in vivo, potato seedlings were inoculated in sterilized soil by soil soak method. Later, mature plants were inoculated in unsterilized soil to find the better resulting compound(s) in field soil condition against the disease. Finally, suspension of cow dung (@25%), propolis (@ 6mg/ml) and turmeric powder (@25%) were selected for trial as soil and seed treatment against the pathogen. It was found that, cow dung reduced 28.89% disease severity index which was followed by 26.67% in propolis and 22.22% in turmeric powder as compared to control (84.44%) in artificially inoculated potato plants against R. solanacearum. J. Biodivers. Conserv. Bioresour. Manag. 2020, 6(1): 65-76

Redox Chemistry and Nutrient Release from Organic Amended Terrace Soil under Anaerobic Incubation

2020 ◽  
pp. 1-12
Author(s):  
Sumaya Sharmin ◽  
Md. Abdul Kader ◽  
Md. Rezwanul Islam ◽  
Muhiuddin Faruquee
Keyword(s):  
Organic Matter ◽  
Organic Materials ◽  
Nutrient Release ◽  
Redox Chemistry ◽  
Cow Dung ◽  
Anaerobic Incubation ◽  
Amended Soils ◽  
Ph Values ◽  
Incubation Study ◽  
Amended Soil

Aims: To examine the changes in pH and Eh values of terrace soils during anaerobic incubation when amended with different organic materials, and to study N, P and S release from different manure and bio-slurry in terrace soil under anaerobic condition. Study Design: The experiment was carried out following Complete randomized design (CRD) with two replications. Place and Duration of Study: A laboratory incubation study was conducted in Soil Science Laboratory, Bangladesh Agricultural University, Mymensingh in December 2014 for 98 days. Methodology: The surface (0-15 cm) soil sample was collected from rice growing field of a selected area of Bhaluka, Mymensingh. The incubation study was carried out using four different sources of organic manures with two replications and five treatment combinations. The changes in soil pH, Eh and release pattern of N, P, and S from some organic materials in terrace soil under anaerobic incubation were investigated during December 2014 to April 2015. The soil was amended with all the treatments at 2g 100 g-1 (air dry basis) soil and incubated for 14 weeks at 25° C. The N, P and S release were determined by the measurement of NH4-N, phosphate P and SO4-S on destructive sampling at every two weeks. Results: The pH values increased at initial stage but gradually decreased over time to neutral and the soil became reduced with the advancement of incubation, it varies (1-3 weeks) depending on the quality of organic matter used. The reduction potential showed a significant variation among the treatments. Overall, when the pH values were averaged over the weeks, the highest pH value was measured in poultry bio-slurry (PB) amended soil followed by poultry manure (PM), cow dung bio-slurry (CDB) and cow dung (CD) amended soils and the lowest was in control. In case of Eh, the most negative (-133.08) Eh value was measured in PM amended soil followed by poultry bio-slurry (PB), CD and CDB amended soils. Control soil had comparatively positive Eh value. At the end of incubation, the highest amount of NH4-N found in CDB followed by CD, PM, PB and the lowest was in control; the highest amount of phosphate P found in PB followed by PM, control, CDB and CD; the highest amount of SO4-S found in PB followed by PM, CD, CDB and the lowest was in control. Conclusion: PB is the best source of organic amendment with respect to release of P and S, whereas CDB showed the best performance in release of N. Nutrient release and availability in reduced environment in terrace soil are a function of soil redox chemistry which is influenced by the quality and quantity of organic matter.

scholarly journals Effects of Gypsum Soil Amendments on Avocado Growth, Soil Drainage, and Resistance to Phytophthora cinnamomi

Plant Disease ◽  
2000 ◽  
Vol 84 (6) ◽  
pp. 612-616 ◽  
Author(s):  
B. J. Messenger ◽  
J. A. Menge ◽  
E. Pond
Keyword(s):  
Organic Matter ◽  
Phytophthora Cinnamomi ◽  
Soil Amendments ◽  
Root Weight ◽  
Infested Soil ◽  
Soil Drainage ◽  
Fresh Weight ◽  
Unamended Soil ◽  
Fresh Organic Matter ◽  
Amended Soil

Infection of avocado seedlings by Phytophthora cinnamomi in infested soil was decreased by 71% by the addition of gypsum soil amendments in replicated greenhouse experiments. Root weights and total seedling weights were not significantly increased by gypsum amendments compared with unamended soil; however, the significant reduction in total seedling weight and root weight caused by P. cinnamomi was largely eliminated by the addition of gypsum. Fresh organic matter amendments alone did not significantly affect the total fresh weight or root weight of avocado seedlings. Root fresh weight was decreased in uninfested soil amended with 5% gypsum and organic matter, but in infested soils, the same treatment increased root fresh weight. Root fresh weight of mature avocado trees in an uninfested grove was not significantly affected by gypsum soil amendments. Avocado seedlings grown in gypsum-amended soil and the roots inoculated with suspensions of Phytophthora cinnamomi zoospores were no more resistant than seedlings grown in unamended soil. Permeability of avocado root membranes, as determined by the amount of 86Rb exuded from root segments over time, was unaffected by growth in gypsum-amended soil. Infiltration of water into soil amended with fine-grade gypsum was impeded initially; whereas soil amended with coarse drywall gypsum drained faster than unamended soil. Drainage was not correlated with root infection of avocados grown in soil infested with P. cinnamomi and amended with gypsum. It appears that large reductions in infection of avocado seedlings by P. cinnamomi in gypsum-amended soil are not caused by an avocado growth response, increased root resistance, or reduced root membrane permeability. Infection is not markedly affected by poor drainage when the soil is amended with high levels of gypsum.

scholarly journals Management of bacterial wilt (Ralstonia solanacearum) of potato: focus on natural bioactive compounds

2018 ◽  
Vol 4 (1) ◽  
pp. 73-92 ◽  
Author(s):  
Z Karim ◽  
MS Hossain
Keyword(s):  
Bioactive Compounds ◽  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Bacterial Pathogen ◽  
Cow Dung ◽  
Broad Host Range ◽  
Cropping Pattern ◽  
Weather Factors ◽  
Contaminated Irrigation Water ◽  
Protective Methods

The bacterial wilt disease caused by Ralstonia solanacearum is an extremely destructive soil borne bacterial pathogen to potato. It appeared as rapid and fatal wilting symptoms in the host. The pathogen entered through different wounds and easily disseminated via infected biological material, soil, contaminated irrigation water, surface water, farm equipment etc. and could survive for many years in association with alternate hosts. It is a widely distributed and very much diversified soil borne pathogen having an unusually broad host range with long-term survivable ability. Direct yield losses caused by the pathogen varied from 30 to 90% depending on different factors such as cultivar, weather factors, soil type, cropping pattern and strain etc. Bacterial wilt continued to be an economically serious problem for field-grown potatoes in many tropical, subtropical and warmer areas of the world including Bangladesh. But the effectiveness of conventional management is limited because of some special biological features of the bacteria. Mostly protective methods and chemical control remain ineffective, antibiotics show hardly any effect, and efficacious biocontrol method has yet to be developed against the organism. However, during the recent decades, some natural bioactive compounds, viz. propolis, honey, turmeric, magnesium chloride, cow dung, aromatic rice extract, iodine, sodium bicarbonate etc. have got attention for their effectiveness in inhibiting a range of serious bacterial pathogens from both Gram positive and Gram negative types. As no conventional method has been found effective alone, such compounds could be tested for their effectiveness against the very successful soil borne bacteria to overcome the traditional management limitations.J. Biodivers. Conserv. Bioresour. Manag. 2018, 4(1): 73-92

scholarly journals EVALUATION OF THREE SURFACE FRICTION TECHNIQUES FOR THE REMOVAL OF ORGANIC MATTER

2015 ◽  
Vol 24 (4) ◽  
pp. 1061-1070 ◽  
Author(s):  
Marcelo Alessandro Rigotti ◽  
Adriano Menis Ferreira ◽  
Mara Corrêa Lelles Nogueira ◽  
Margarete Teresa Gottardo de Almeida ◽  
Odanir Garcia Guerra ◽  
...  
Keyword(s):  
Organic Matter ◽  
Adenosine Triphosphate ◽  
Organic Material ◽  
Surface Friction ◽  
Disinfection Process ◽  
Significant Difference ◽  
Before And After ◽  
Quantitative Descriptive ◽  
The One ◽  
Cleaning And Disinfection

ABSTRACT The objective of this study was to assess the effectiveness of three surface friction techniques for the removal of organic material. A quantitative, descriptive and exploratory study was developed to evaluate the presence or not of organic material before and after the cleaning and disinfection process of surfaces of bedside tables of patients hospitalized at an Intensive Care Unit. Three friction techniques were executed in the one-way, two-way and centrifugal sense, individually, three times on each table, during alternate weeks. For each patient unit and friction technique, a single table and three sides of cloth were used, moistened with 70% (w/v) alcohol. The organic matter was detected through the presence of adenosine triphosphate by bioluminescence, using 3M(tm) Clean-Trace(tm) ATP Systems. For each technique, 13 samples were collected before and 13 after the cleaning/disinfection process, totaling 78 samples of adenosine triphosphate by bioluminescence. No statistically significant difference was found among the removal techniques of organic matter. This study demonstrated that none of the three surface friction methods was better than the other to remove organic matter. Nevertheless, further research is needed in which other cleaning/disinfection indicators and surfaces are considered.

scholarly journals Pengaruh Sumber dan Dosis Bahan Organik terhadap Perubahan sifat Kimia Tanah, Pertumbuhan dan Hasil Tanaman Sorgum (Sorghum bicolor L.Moench) var. KD4 di Lahan Kering Jatikerto, Malang

2019 ◽  
Vol 7 (2) ◽  
Author(s):  
Nur Edy Suminarti ◽  
A.Y. Edy Guntoro ◽  
A. N. Fajrin
Keyword(s):  
Organic Matter ◽  
Sorghum Bicolor ◽  
Leaf Area ◽  
Chemical Properties ◽  
Dry Weight ◽  
Soil Chemical Properties ◽  
Growth And Yield ◽  
Cow Dung ◽  
Dry Land ◽  
Total Dry Weight

Suminarti et al, 2018. Effect of Source and Dosage of Organic Materials on Changes in Soil Chemical Properties, Growth and Yield of Sorghum Plants (Sorghum bicolor L.Moench) var. KD4 in Dry Land Jatikerto, Malang. JLSO 7(2): Agricultural extensification is the right step to anticipate conditions of food insecurity. This refers to two reasons, namely (1) proliferation of land conversion activities, and (2) sorghum is a carbohydrate-producing plant that is quite tolerant when planted on dry land. The objective of this study was to obtain information about the sources and doses of organic matter that are appropriate to changes in soil chemical properties, growth and yield of sorghum plants, and has been carried out in the dry land of Jatikerto, Malang. A split plot designs with three replications were used in this study, sources of organic material (blothong, UB compost and cow dung) as the main plot, and doses of organic matter (125%, 100% and 75%) as a subplot. Soil analysis was carried out 3 times, i.e. before planting, after application of organic matter and at harvest. The agronomic observations were carried out destructively at 80 days after planting (DAP) including the components of growth (root dry weight, leaf area, and total dry weight of the plant) and harvest at the age of 90 DAP.F test at 5% level was used to test the effect of treatment, while the difference between treatments was based on LSD level of 5%.The results showed that there was a significant interaction between the source and dosage of organic matter on the leaf area and total dry weight, the highest yield was obtained in blothong at various doses. Higher yields of seeds per hectare were also found in blotong: 1.76 tons ha-1, and 1.73 tons ha-1 on 125% doses of organic matter. Blotong application is able to provide elements of N, P and K soil respectively 18.3%, 85.68% and 8.42% for plant.

scholarly journals Accumulation of total nutrients, dry matter and phytochrome content in Boesenbergia stenophylla R.M Smith as affected by different light conditions

2021 ◽  
pp. 10-16
Author(s):  
Tracy Saptu ◽  
Nur Ashikin Psyquay Abdullah ◽  
Franklin Ragai Kundat ◽  
Aicher Joseph Toyat ◽  
Calson Gimang Endawie ◽  
...  
Keyword(s):  
Organic Matter ◽  
Nutrient Uptake ◽  
Chlorophyll Content ◽  
Dry Matter ◽  
Application Rate ◽  
Dry Matter Yield ◽  
Forest Canopies ◽  
Soil Mixture ◽  
Significant Difference ◽  
Phytochrome Content

A study was conducted to determine the best agronomic practices for the cultivation of jerangau merah as a new medicinal crop. Jerangau merah is scientifically known as Boesenbergia stenophylla is a ginger plants that is highly endemic to the Borneo Highland. This understorey herb grows in cluster and under the heavy shades of forest canopies and perceived only the sunflecks that pass through the forest canopies. Jerangau Merah has been consumed by the locals for its medicinal values since decades ago. Generally, B. stenophylla is well known for its function as antidote for alcohol. Moreover, the B. stenophylla is also beneficial for rheumatic pains, remedies for stomach ache and toothache and as antiseptic wash as well. This herb is not propagate by the locals due to its sensitivity to sunlight and lack of agronomic information for B. stenophylla. Harvesting it from their natural habitat, however, seen the decline it its population size hence cultivation is essential. This paper aimed to determine the total nutrients, dry matter yield and phytochrome content of Bosenbergia stenophylla R.M. Smith under different light regimes. A study for determining suitable planting media for B. stenophylla was conducted at Universiti Putra Malaysia (UPM). The treatments are: i) (M1) with the ratio of 3:2:1 top soil: sand: organic matter, media ii) (M2) consists of soil mixture 3:2:1 placed in water-logged polyethylene bags and media iii) (M3) contained only leaf litters. The results showed that the herbs showed significantly higher number of shoots and leaves in the common soil mixture. But, better root development in media containing leaf litters. The study proceeded with field experiment at Ba’Kelalan to determine the effect of different shade levels and fertilizing regimes on growth. B. stenophylla was cultivated under two different levels of shade cloths: 70% and 90% level of shade and different fertilizing regimes (T1 as control, no fertilizer applied to the plants; T2, chicken dung; T3, NPKMg and T4, mixed of chicken dung and NPKMg). The study for determining the effects of different shade level and fertilizing regimes on seedling’s growth which conducted at Ba’Kelalan in factorial randomized completely blocked design (RCBD). The data collected for 9 weeks of planting which include nutrient content in soil, leaf, PAR and phytochrome content and growth parameters. Regarding the nutrient uptake, the results showed no interactions between fertilizing regimes and shade levels. There were no significant different in nutrient elements except for magnesium and potassium. Magnesium is essential for the formation of chlorophyll which ensure efficiency of photosynthesis when under higher light intensity. Among all the fertilizer treatment, it was showed that chicken dung amendment has higher nutrient uptake. Thus, it is recommended chicken dung should be added into the NPKMg for better nutrient uptake. Further study on suitable fertilizer rates apply to B. stenophylla should be taken into consideration. Moreover, plants cultivated under 70% have been higher and have higher dry matter yield than those plants cultivated under 90%. The result also revealed that there was significant different in chlorophyll content of B. stenophylla cultivated under 70% which treated either with chicken dung and NPKMG respectively. However, plants under 90% have higher chlorophyll content than those under 70%. Based on the results, plants under 70% shade was taller than those under 90% and there were significant difference in height among treatments under 70%.  It was observed plant treated with NPKMg was taller. This study showed that shade and fertilizers significantly affected the dry matter yield of B. stenophylla. Moreover, adding NPKMg to the treatments yields more dry matter content of jerangau merah. As for phytochrome content, there was no significant effect of fertilizer on phytochrome content. However, there was significant difference among the shade levels. 90% shade showed higher phytochrome content than those under 70%. In overall, both shade and fertilizer is important in cultivation of Jerangau Merah. 70% shade was observed to have significant effects on growth of jerangau merah and also more economical compared to 90%. Moreover, combination of organic matter and NPKMg also promote the growth of jerangau merah enhance the nutrient uptake efficiency of jerangau merah. However, further investigation of suitable fertilizer and application rate are required to determine suitable fertilizer for jerangau merah and application rate for optimum growth of jerangau merah.

scholarly journals Protura (Arthropoda: Hexapoda) in Slovenian caves

2021 ◽  
Vol 50 (1) ◽  
pp. 65-74
Author(s):  
Loris Galli ◽  
Franc Janžekovič ◽  
Peter Kozel ◽  
Tone Novak
Keyword(s):  
Organic Matter ◽  
Environmental Conditions ◽  
New Records ◽  
Species Level ◽  
Statistical Analyses ◽  
Small Class ◽  
Surface Soils ◽  
Significant Difference ◽  
Unidentified Species ◽  
Scarce Data

Protura is a small class of Hexapoda, generally poorly known, and rather scarce data on its occurrence in caves are scattered throughout literature on fauna in caves and in some papers on Protura. Although the cave-dwelling fauna is relatively well studied in Slovenia, published records on Protura are rare. In this paper, data on the occurrence and abundance of Protura in Slovenian caves are considered. Various statistical analyses were performed to compare caves with Protura to those without in 60 intensively monitored cavities to detect any differences in the selected environmental conditions. No significant difference was obtained. Samples collected from 15 caves yielded 286 specimens identified to genus or species level. Ten species were identified: Acerentulus confinis (Berlese, 1908), A. rafalskii Szeptycki, 1979*, Acerentomon affine Bagnall, 1912*, A. balcanicum Ionesco, 1933*, A. italicum Nosek, 1969, A. maius Berlese, 1908*, A. meridionale Nosek, 1960, Acerella muscorum (Ionesco, 1930)*, Eosentomon armatum Stach, 1926*, and E. transitorium Berlese, 1908*. The seven species marked with an asterisk are new records for Slovenia. Two specimens belonging to an unidentified species of the genus Ionescuellum Tuxen, 1960 were also found. As expected, Protura were most abundant at the cave entrance close to the surface, and none of the species were found exclusively in cavities. This suggests that proturans were introduced passively into the cavities via organic matter and surface soils.

scholarly journals A study of Soil Invertebrates community in a Date - Palm plantation in Baghdad, Iraq

2016 ◽  
Vol 13 (1) ◽  
pp. 1-6
Author(s):  
Baghdad Science Journal
Keyword(s):  
Organic Matter ◽  
Direct Method ◽  
Chemical Properties ◽  
Essential Elements ◽  
Soil Samples ◽  
Land Snails ◽  
Soil Invertebrates ◽  
Individual Number ◽  
Significant Difference ◽  
Physical And Chemical

Soil invertebrates community an important role as part of essential food chain and responsible for the decomposition in the soil, helps soil aeration , nutrients recycling and increase agricultural production by providing the essential elements necessary for photosynthesis and energy flow in ecosystems.The aim of the present study was to investigate the soil invertebrates community in one of the date palms plantation in Aljaderia district South of Baghdad, , and their relationships with some physical and chemical properties of the soil , as Five randomly distributed replicates of soil samples were collected monthly. Invertebrates samples were sorted from the soil with two methods, direct method to isolate large invertebrates and indirectly to isolate small invertebrates using wet funnel method. The study also included the determination of physical and chemical factors of the soil (Temperature, Salinity, pH, Organic matter, Humidity, In addition to the soil texture).Monthly fluctuations in physical and chemical characteristics of the soil and the total invertebrates community study site were determined. Significant correlations the of the invertebrates community and each of temperature, organic matter, and humidity were observed. The study revealed that the temperature of the soil ranged between 5 to 25 C0 , The salinity concentration ranged between 1.1-1.9 ‰, The pH values ranged between 7.3 to 7.8 and the percentage of soil moisture ranged between 15 - 25% , Soil samples were composed of 44.6 % Clay, 19.7% Silt and 35.5% Sand.A total of 4625 individuals of soil invertebrates belonging to 16 taxa were sorted , within which the adult and larval insects were the most abundant, and from them 1283 individuals were sorted , represented 28% of the total numbers, followed by Isopoda , which 1030 individuals of them were sorted, In addition to Nematode, Oligochaetes Annelids family Enchytraeidae, and Earthworms family Lumbricida, Species of Chilopoda, Diplopoda, mites, land snails and slugs. The highest total individual number were recorded recorded durim moderate temperature months, February, March and April amounted to 838, 801 and 813 individuals, respectively.A significant correlation was mated between total number of soil invertebrates and each of temperature, organic matter and humidity. The significant difference in means was calculated according to LSD test.

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