scholarly journals The study of fillet proximate composition, growth performance and survival rate of striped catfish (Pangasius hypophthalmus) fed with diets containing different amounts of alpha-tocopherol (Vitamin-E)

2014 ◽  
Vol 20 ◽  
pp. 67-74 ◽  
Author(s):  
Syeda Mushahida Al-Noor ◽  
Md Delwer Hossain ◽  
Md Amirul Islam

Context: Alpha-tocopherol has significant effect on the growth performance and fillet proximate composition of Striped Catfish (Pangasius hypophthalmus). Objectives: To study the effect of DL-alpha tocopheryl acetate on the fillet proximate composition (moisture, crude protein, crude lipid, ash and alpha-tocopherol), survival rate and growth performance (SGR, weight gain, PWG) of P. hypophthalmus. Materials and methods: Striped Catfish were fed with three trial diets (diet-1, diet-2 and diet-3) were prepared by adding different amounts of alpha-tocopheryl acetate (ATA, 0, 90 and 300 mgKg-1 diet), over a period of 122 days. In each pond, 10% of the total fish were randomly selected and individually weighed once every fortnight and growth parameters were determined after each weighing. Moisture, dry matter, crude protein, lipid, ash and alpha-tocopherol of the experimental diets and fish fillet were determined by Conventional procedure, standard test method, micro-kjeldahl method, Bligh and Dyer method, standard methods and chromatographic method, respectively. Results: Fish that were fed 90 mg kg-1 and 300 mg kg-1 ATA supplemented diets had significantly higher growth performance than fish that were fed only farm processed diet (P<0.05). The fillet protein, lipid content and alpha tocopherol level of the fish flesh were increased due to ATA supplementation in diets. The vitamin-E levels in fish fillet reflected dietary ATA levels. These results showed that fish on Diets 2 (supplemented with 90 mgKg-1 ATA) and Diet 3(supplemented with 300 mg kg-1 ATA) had significantly better production and resulted better flesh quality of fish than those on Diet 1 (without ATA supplementation). Conclusion: Scanty information is available on the fillet proximate composition and growth performance of P. hypophthalmus fed with diets containing different amounts of alpha-tocopherol. The present study provides baseline information for establishing improved and profitable cultured system of the fish in impounds water of Bangladesh. DOI: http://dx.doi.org/10.3329/jbs.v20i0.17658 J. bio-sci. 20: 67-74, 2012

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 202-203
Author(s):  
Ding Wang ◽  
Young Dal Jang ◽  
G K Rentfrow ◽  
H J Monegue ◽  
M J Azain ◽  
...  

Abstract The study objective was to assess the contribution of fat source and vitamin E (VE) supplementation on growth performance, antioxidant status, and fatty acid profile in the liver of pigs at heavy slaughter weight (148.09 ±1.64 kg). A total of 72 individually-fed pigs (36 barrows, 36 gilts; 28.55 ±1.16 kg) were randomly assigned to 12 dietary treatments in a 2 × 6 factorial arrangement. Fat treatments were tallow and corn-oil. The VE treatments included four levels of α-tocopheryl-acetate (ATA; 11, 40, 100, and 200 ppm) and two levels of mixed tocopherols (primarily γ-tocopherol; 40 and 100 ppm). Liver samples were collected at slaughter. Data analysis were performed by ANOVA using GLM in SAS. No effect of dietary fat sources, isoforms of VE, and levels of γ-tocopherol were detected on growth performance or liver antioxidant status (GSH, GSSG, SOD, and MDA). Increasing dietary ATA linearly increased overall ADG (0.98, 1.02, 1.02, and 1.08 kg/day; P = 0.02) from 28 to 150 kg; overall ADG of pigs fed γ-tocopherol-diets was 1.07 and 1.04 kg/day, respectively. An interaction between fat sources and ATA was observed on cumulative ADG during Phase1-3 (28-100 kg; P = 0.04) and Phase1-4 (28-125 kg; P = 0.03) wherein pigs fed corn-oil-diet, but not tallow-diet, had increased ADG with increased dietary ATA. Increasing dietary ATA quadratically increased SOD activity (P < 0.05; highest at 100 ppm), and quadratically decreased MDA content (P < 0.05; lowest at 40 ppm) in the liver. Liver fatty acid profile was affected by fat source (P < 0.05) but not VE treatment, pigs fed corn-oil-diets had less SFA (34.23 vs. 35.45%; P = 0.02) and MUFA (11.98 vs. 18.38%; P < 0.0001), but more PUFA (49.14 vs. 40.78%; P < 0.0001) than pigs fed tallow-diets in the liver. Beneficial effects of dietary VE supplementation were observed on growth performance and antioxidant status of pigs under conditions of this study.


1999 ◽  
Vol 69 (2) ◽  
pp. 92-95 ◽  
Author(s):  
Chopra ◽  
Bhagavan

There are several reports in the literature on the relative bioavailabilities of RRR (natural) vs. all-rac (synthetic) forms of vitamin E in humans and animal models but none on the bioavailability of alpha-tocopherol in mixed vitamin E formulations. In the present study we examined the bioavailability of alpha-tocopherol in a typical commercially available product containing mixed tocopherols. We also tested a formulation containing all-rac-alpha-tocopherol with mixed tocopherols for purposes of comparison along with straight RRR-and all-rac-alpha-tocopheryl acetate as reference products. Normal male subjects were given one of the four formulations of vitamin E (800 IU per day in softgel capsule form for 10 days): 1. All-rac-alpha-tocopheryl acetate, 2. RRR-alpha-tocopheryl acetate, 3. RRR-alpha-tocopherol with mixed tocopherols, and 4. all-rac-alpha-tocopherol with mixed tocopherols. Both serum alpha- and gamma-tocopherols were determined by HPLC at baseline, and at days 2, 4, 7 and 10. The values for alpha- at baseline and 10 days were 0.80, 0.80, 0.80 & 0.79 mg/dl and 1.67, 1.72, 1.76 & 1.62 mg/dl. The values for gamma- were 0.28, 0.29, 0.30 & 0.29 mg/dl and 0.11, 0.08, 0.10 & 0.10 mg/dl. Thus the data show that a) the bioavailability of RRR-and all-rac-alpha-tocopherols is not affected by other tocopherols, and b) both RRR-and all-rac-alpha-tocopherol (free or esterified) significantly suppress the serum gamma tocopherol to the same extent. Furthermore, since there was no difference in the serum values of alpha-tocopherol between RRR-and all-rac-vitamin E given the same dose as IUs, the data also support the currently accepted ratio of 1.36 for the biopotency of RRR- vs. all-rac-alpha-tocopheryl acetate.


2020 ◽  
Vol 65 (No. 10) ◽  
pp. 380-388
Author(s):  
Antonella Dalle Zotte ◽  
Marco Cullere ◽  
Elizabeth Gleeson ◽  
Maria Elena Cossu

The present study tested the effect of a dietary inclusion with vitamin E and pork lard on the apparent digestibility of the nutrients, the growth performance, the carcass traits, the physical meat quality, and the α-tocopherol content. A total of 60 hybrid rabbits were reared in individual cages from weaning (35 days of age) until slaughter (78 days of age). A control diet with no supplements, one diet supplemented with 2% pork lard, and two diets that used the aforementioned diets supplemented with an additional 200 mg/kg α-tocopheryl acetate were designed. The diets were isoprotein and isoenergy. The fat inclusion increased the crude protein (P &lt; 0.05) and ether extract (P &lt; 0.001) total tract apparent digestibility, and the same was observed for the vitamin E inclusion (P &lt; 0.001 for both variables). This improved the dietary digestible protein content (P &lt; 0.05), which increased the digestible protein to digestible energy ratio (P &lt; 0.001). The fat × vitamin E interaction was observed for the total tract apparent digestibility of the ether extract (P &lt; 0.001), the neutral detergent fibre (P &lt; 0.05) and the acid detergent fibre (P &lt; 0.01). The growth traits were unaffected, with the exception of the feed conversion ratio that improved with the vitamin E addition (P &lt; 0.05). Similarly, the carcass traits remained unaffected, with the exception of the perirenal and total fat incidence that increased with the fat supplement (P &lt; 0.05), and the scapular fat that was reduced with the vitamin E inclusion (P &lt; 0.05). The meat L* (lightness), a* (redness), b* (yellowness) colour values and ultimate pH were unaffected by the experimental treatments, even though a fat × vitamin E interaction was observed for the a* and chroma values of the Longissimus thoracis et lumborum muscle (P &lt; 0.05). Both the fat (P &lt; 0.05) and vitamin E (P &lt; 0.001) dietary inclusion increased the meat α-tocopherol content. Based on the results, it was concluded that the 2% dietary inclusion of animal fat did not provide more benefits for the considered parameters than the sole α-tocopheryl acetate incorporation, but contributed to the increase in the vitamin E content in the meats.


2019 ◽  
Vol 11 (1) ◽  
pp. 89-96
Author(s):  
RUDY AGUNG NUGROHO ◽  
ESTI HANDAYANI HARDI ◽  
YANTI PUSPITA SARI ◽  
RETNO ARYANI ◽  
RUDIANTO RUDIANTO

Abstract. Nugroho RA, Hardi EH, Sari YP, Aryani R, Rudianto. 2019. Growth performance and blood profiles of striped catfish (Pangasianodon hypophthalmus) fed leaves extract of Myrmecodia tuberosa. Nusantara Bioscience 11: 89-96. The application of plant extract in the aquaculture field is gaining momentum. This study aimed to determine the effects of M. tuberosa Jack leaves extract on the growth performance and blood profiles of striped catfish (Pangasianodon hypophthalmus). 400 fish with an initial average weight of 1.54 ± 0.02g were randomly divided into five groups and fed with different concentration of M. tuberosa Jack leaves extract (0.25, 0.5, 1, 2%) and control diet for 80 days. Growth performance, feed conversion rate, feed efficiency, survival rate, morphometric, and hematological profile of the fish were evaluated at the end of the trial. The results found that fish fed 0.5-1% concentration had significantly higher (P<0.05) growth, feed conversion rate, feed efficiency, morphometric value, survival, white blood cell, hemoglobin, neutrophil, lymphocyte, and monocyte than control. Meanwhile, supplementation of 2% MTE in the diet of fish reduced growth, feed utilization, viscerosomatic index, and morphometric values but increased red blood cell. All the supplemented diets decreased platelet of fish. The findings indicated that supplementation 0.5-1% of MTE is beneficial to the growth and blood profile of fish through the increment of growth indices, feed utilization, white blood cell, hemoglobin, neutrophil, lymphocyte, and survival rate of fish.


F1000Research ◽  
2017 ◽  
Vol 6 ◽  
pp. 1545 ◽  
Author(s):  
Iskandar Putra ◽  
Rusliadi Rusliadi ◽  
Muhammad Fauzi ◽  
Usman M. Tang ◽  
Zainal A. Muchlisin

Background The objective of the present study was to evaluate the growth performance and feed utilization of African catfish Clarias gariepinus fed a commercial diet and reared in the biofloc system enhanced with probiotic. Methods The treatment was the frequency of probiotic application into the cultured system, namely, 5-day interval, 10-day interval, and 15-day interval for 60 days of experiment. Biofloc culture was grown in an experiment tank (vol. 2000 L) by mixing the probiotic (Bacillus sp.) 10 mL and molasses 200 mL per liter of water.  The fish was stocked into the biofloc system 7 days after cultured at stocking density of 1000 fish tank-1.  The fish was fed a commercial diet that contains 38% crude protein, twice a day at satiation. The application of probiotic was reperformed after 5 days, 10 days, and 15 days after stocking. Results The study showed that the growth performance, survival, and feed utilization of African catfish were higher in the treatment at 5-day intervals over 60 days. The ANOVA test showed that the application frequency of probiotic into biofloc system of cultured media had the significant effect on the growth performance, survival rate, and feed utilization of African catfish. Conclusion The best growth performance and feed utilization were  found at the application of probiotic into biofloc system at 5-day intervals over 60 days.


2013 ◽  
Vol 20 (1-2) ◽  
pp. 107-116
Author(s):  
MFA Mollah ◽  
A Roy ◽  
MSA Mamun

An experiment to observe the growth performance of the larvae produced from female Clarias batrachus treated with 4 dietary levels of vitamin E viz. 0 mg, 50 mg, 100 mg, and 200 mg /kg feed was conducted for 28 days. After rearing for 3 months females were used for induction of breeding to produce larvae. Same dose of pituitary gland (100 mg PG/kg body weight) was used for all treatments. From the 7th day of hatching, the larvae were reared for another period of 28 days to see growth and survival rate of the larvae produced by the broods maintained under different dietary levels of vitamin E. In case of growth performance (percent length gain, percent weight gain, specific growth rate) and health condition larvae of T2 (50 mg vitamin E/kg feed) showed significantly better result when compared with those of T1 and T3. There was no significant difference in the growth performance between the larvae of T2 and T4. Larvae of T2 although showed higher survival rate but the difference between different treatments was insignificant. The results concluded that 50 mg vitamin E/kg feed is more suitable to increase growth and survival rate of the larvae.DOI: http://dx.doi.org/10.3329/pa.v20i1-2.16862 Progress. Agric. 20(1 & 2): 107 – 116, 2009


1996 ◽  
Vol 76 (1) ◽  
pp. 63-72 ◽  
Author(s):  
M. J. Garber ◽  
R. A. Roeder ◽  
W. M. Pumfrey ◽  
G. T. Schelling ◽  
P. M. Davidson

This study examined dose-response effects of supplemental dietary dl-α-tocopheryl acetate (VE) on growth performance, immune response, and carcass and meat sensory characteristics in two breeds of cattle. Seventy-five crossbred beef steers (361 ± 22 kg) were blocked by weight, randomly assigned to one of five treatment groups, and fed a ration supplemented with 0, 250, 500, 1000 or 2000 IU VE steer−1 d−1. Sixty Holstein steers (366 ± 22 kg were similarly blocked and allocated (no 250 IU treatment). Four days post-slaughter the left sirloin subprimal of each steer was removed and vacuum packaged. On day 13, small cores were cut from the gluteus medius (GM) and packaged with O2-permeable wrap; these were then displayed under 45-W natural white light for up to 10 d at 4 °C. Serum α-tocopherol increased (P < 0.0001) with increasing VE in both breeds, although more so (P < 0.0001) in beef steers. The GM α-tocopherol levels increased linearly (P < 0.0001) with increasing VE, but more so (P < 0.0002) in beef steers. The levels of α-tocopherol in semimembranosus muscle, perirenal and subcutaneous fat, and liver also increased linearly (P < 0.0001) in both breeds. Growth performance of all steers and carcass characteristics of beef steers were not affected (P > 0.10) by VE. Dairy steers receiving 1000 IU d−1 had darker (P < 0.03) ribeyes. There were no effects (P > 0.10) of VE on sensory attributes of frozen steaks. Surface metmyoglobin formation was deterred by VE supplementation in meat from beef (linear, P < 0.016) but not dairy (P > 0.10) steers. Lipid oxidation was suppressed (linear, P < 0.0002) by VE but more so (P < 0.0001) in beef than in dairy meat (72.9% vs. 40.0% suppression, respectively, at 2000 IU d−1). Immune response increased (P < 0.074) at higher levels of VE. In summary, VE supplementation improved immune response and increased serum and muscle α-tocopherol levels, resulting in delayed metmyoglobin formation (beef) and lipid oxidation (beef and dairy) in a dose-dependent manner. Supplemental VE was more effective in meat from beef steers than in that from dairy steers. Key words: Vitamin E, immunity, growth, carcass


2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Alison M. Mondul ◽  
Steven C. Moore ◽  
Stephanie J. Weinstein ◽  
Anne M. Evans ◽  
Edward D. Karoly ◽  
...  

Background. The Alpha-Tocopherol, Beta-Carotene Cancer Prevention (ATBC) Study, a randomized controlled cancer prevention trial, showed a 32% reduction in prostate cancer incidence in response to vitamin E supplementation. Two other trials were not confirmatory, however.Objective. We compared the change in serum metabolome of the ATBC Study participants randomized to receive vitamin E to those who were not by randomly selecting 50 men from each of the intervention groups (50 mg/day all-rac-α-tocopheryl acetate (ATA), 20 mg/dayβ-carotene, both, placebo).Methods. Metabolomic profiling was conducted on baseline and follow-up fasting serum (Metabolon, Inc.).Results. After correction for multiple comparisons, five metabolites were statistically significantly altered (βis the change in metabolite level expressed as number of standard deviations on the log scale):α-CEHC sulfate (β=1.51,p=1.45×10-38),α-CEHC glucuronide (β=1.41,p=1.02×10-31),α-tocopherol (β=0.97,p=2.22×10-13),γ-tocopherol (β=-0.90,p=1.76×10-11), andβ-tocopherol (β=-0.73,p=9.40×10-8). Glutarylcarnitine, beta-alanine, ornithine, and N6-acetyllysine were also decreased by ATA supplementation (βrange 0.40 to −0.36), but not statistically significantly.Conclusions. Comparison of the observed metabolite alterations resulting from ATA supplementation to those in other vitamin E trials of different populations, dosages, or formulations may shed light on the apparently discordant vitamin E-prostate cancer risk findings.


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