scholarly journals Correlation Between Oxidative Stress Generation, Reticular Fiber Density and Testicular Histomorphometric Parameters Of Streptozotocin-Induced Diabetic Wistar Rat

2021 ◽  
Vol 29 (2) ◽  
pp. 1-8
Author(s):  
GE Anyanwu ◽  
CA Agbor
Keyword(s):  
Oxidative Stress ◽  
Blood Glucose ◽  
Wistar Rat ◽  
Stress Generation ◽  
Fiber Density ◽  
Glucose Levels ◽  
Stress Markers ◽  
Blood Glucose Levels ◽  
Reticular Fiber ◽  
Tubular Diameter

The actual underlying mechanism of alterations in testicular histomorphometric parameters resulting from diabetes-induced oxidative stress is still not well understood because of the absence of supporting evidence from scientific experiments. This study was conducted to investigate the correlation between serum oxidative stress markers with testicular histomorphometric parameters and reticular fiber density of Streptozotocin-induced diabetic Wistar rat. The experiment included twenty eight adult male rats sorted into four groups, Group A (Control), other animals were sorted into treatment groups based on their blood glucose levels after inducing diabetes with 65 mg/kg/bw of streptozotocin, Groups B (100 - 200 mmol/l), Group C (210 - 250 mmol/l), Group D (260 - 300 mmol/l). At termination, Serum superoxide dimutase (SOD), catalase and melondialdehyde where evaluated using reagent based antioxidant enzyme assay while reticulum stain kits was used to demonstrate for reticular fiber density. Histomorphometric measurements were carried out using ocular micrometer after calibration on a light microscope. Statistical analysis was done using analysis of variance with p<0.05 considered significant. Results reveal that the higher the blood glucose levels in diabetic animals, the higher the serum concentration of oxidative stress markers. Density of reticular fiber increased with increase in blood glucose levels, while tubular diameter and epithelial height decreased with increase in increase in hyperglycaemic levels. In conclusion, there was a progressive increase in reticular fiber density and decrease in tubular diameter and epithelial height as a consequence of increase oxidative stress generation in diabetic model. J. Bio-Sci. 29(2): 01-08, 2021 (December)

scholarly journals STREPTOZOTOCIN-INDUCED OXIDATIVE STRESS IN DIABETIC RATS - A DEFENSIVE EFFECT OF PSYDRAX DICOCCOS

2018 ◽  
Vol 11 (11) ◽  
pp. 378
Author(s):  
Ravi Kumar V ◽  
Sailaja Rao P
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Enzymes ◽  
Blood Glucose ◽  
Methanolic Extract ◽  
Antioxidant Effect ◽  
Control Group ◽  
Standard Drug ◽  
Antihyperglycemic Activity ◽  
Glucose Levels ◽  
Blood Glucose Levels

Objective: The present study was aimed to evaluate the antihyperglycemic activity and in vivo antioxidant effect of methanolic extract of whole plant of Psydrax dicoccos (MEPD) belonging to the family Rubiaceae.Methods: MEPD was prepared by Soxhlet extraction. Wistar rats weighing (180–200 g) were divided into six groups (n=6), with three doses of 100 mg/kg, 200 mg/kg, and 400 mg/kg of extract. Metformin was used as a standard drug. Diabetes was induced by streptozotocin (STZ) (40–50 mg/kg, i.p) in control group. The animals were treated with different doses of extracts for 21 days, and on the 22nd day, the blood glucose levels along with antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), and lipid peroxidase (LPO) were determined.Results: The phytochemical screening of the extract showed the presence of carbohydrates, phenolics, flavonoids, glycosides, and tannins. The methanolic extract of MEPD at the dose of 200 mg/kg body weight showed a significant reduction in blood glucose levels (**p<0.001) with the value of 151.2 mg/dl on the 22nd day at 8 h. A promising antioxidant effect was also evident from the determination of antioxidant enzymes such as SOD, CAT, and LPO.Conclusion: The P. dicoccos extract revealed a potential effect of antihyperglycemic activity and combating nature on oxidative stress induced by STZ.

scholarly journals Aerobic Exercise Improves Sepsis via Impairing LPS-induced Lactate and HMGB1 Release in Mice

2021 ◽  
Author(s):  
xishuai Wang ◽  
zhiqing Wang ◽  
donghui Tang
Keyword(s):  
Oxidative Stress ◽  
Blood Glucose ◽  
Aerobic Exercise ◽  
Mrna Expression ◽  
Aerobic Glycolysis ◽  
Aortic Injury ◽  
Organ Injury ◽  
Stress Injury ◽  
Glucose Levels ◽  
Blood Glucose Levels

Abstract Purpose: In the present study, we attempted to investigate whether aerobic exercise (AE) could prevent sepsis and its complications and explored the related mechanisms. Methods: Forty ICR mice were divided into four groups: Control (Con), Lipopolysaccharide (LPS), Exercise (Ex), and Exercise + LPS (Ex + LPS) groups. Ex and Ex + LPS mice were performed with low-intensity AE for 4 weeks. LPS and Ex + LPS mice received 5 mg/kg LPS intraperitoneally for induction of sepsis. Histopathological micrographs showed the organ injury. This study examined the effects of AE on LPS-induced changes in systemic inflammation, pulmonary inflammation, lung permeability, oxidative stress-related indicators in the lung, blood glucose levels, plasma lactate levels, and plasma high-mobility group box 1 (HMGB1) levels, and bronchoalveolar lavage fluid (BALF) cell count. Sixty mice were used to perform survival rate analysis. Results: AE improved survival rates, MODS, and aortic injury in mice with sepsis. AE decreased LPS-induced oxidative stress injury in lung tissue. AE reduced the infiltration of neutrophils in the lung, liver, kidney, and heart tissues. AE suppressed CXCL-1, CXCL-8, IL-6, and TNF-α mRNA expression but activated IL-1RN, IL-10, Sirt-1, and Nrf-2 mRNA expression in the lung. AE decreased the serum levels of lactate and HMGB1 but increased blood glucose levels during sepsis. Conclusions: AE improves sepsis-associated lung, liver, kidney, heart, and aortic injury and death. AE modulates the inflammatory-anti-inflammatory and oxidative-antioxidative balance in the lung. AE, which can regulate the Warburg effect and impair LPS-induced lactate and HMGB1 release, is a novel therapeutic strategy for sepsis targeting aerobic glycolysis.

scholarly journals Therapeutic Effect of Red Spinach (Amaranthus tricolor L.) Extract on Pancreatic MDA Levels Rats (Rattus norvegicus) Exposed to MLD-STZ

2021 ◽  
Vol 7 (3) ◽  
pp. 129-133
Author(s):  
Kartika Rahma ◽  
Ois Nurcahyanti
Keyword(s):  
Oxidative Stress ◽  
Blood Glucose ◽  
Blood Sugar ◽  
Rattus Norvegicus ◽  
Amaranthus Tricolor ◽  
Glucose Levels ◽  
White Rats ◽  
Blood Glucose Levels ◽  
Spinach Extract ◽  
Sugar Levels

Background: Several studies have reported that an increase in blood glucose in patients with diabetes mellitus (DM) can cause complications to death that occur due to oxidative stress conditions due to excessive ROS production. Red spinach extract was found to be rich in antioxidant compounds. However, there has been no further research on the ability of red spinach to lower blood glucose levels and prevent oxidative stress, which can be seen from the levels of malondialdehyde (MDA; a marker of oxidative stress) in the DM body.Objective: This study aims to determine whether there is a change in blood glucose levels and MDA levels in DM animal models between the groups that were given red spinach extract and not.Methods: This study used white rats (Rattus norvegicus) which were divided into 5 groups: K(-) group in which the rats were not induced by diabetogenic agent and were not treated; K(+) group, the rats were made DM induced by multiple low dose streptozotocin (MLD-STZ); and T1, T2, and T3 groups were exposed to MLD-STZ and treated with red spinach extract (Amaranthus tricolor L.) at a dose of 200 mg/kgBW, 300 mg/kgBW and 400 mg/kgBW, respectively. Blood sugar levels were checked before and after treatment with extract using glucometer digital. Meanwhile, MDA levels were measured by TBA test using protein isolates from the pancreas of each rat.Results: The results showed that the average MDA levels in the K-, K+, T1, T2, and T3 groups were 1.759±0.08, 2.280±0.15, 2.303±0.11, 1.927±0.06, and 1.801±0.04. While the average blood sugar levels in the K-, K+, T1, T2, and T3 groups were 114.4±8.82, 464±72.78, 421.2±37.60, 140.6±20.19, and 176±13.06.Conclusion: It can be concluded that the administration of red spinach extract therapy in DM model rats was able to reduce both glucose levels and MDA levels. By lowering the levels of MDA compounds, it is also believed to be able to prevent oxidative stress in cells which causes tissue damage.

Comment on: acute and chronic fluctuations in blood glucose levels can increase oxidative stress in type 2 diabetes mellitus

2012 ◽  
Vol 50 (5) ◽  
pp. 825-826
Author(s):  
Turgay Ulas ◽  
Mehmet Sinan Dal ◽  
Irfan Tursun ◽  
Mehmet Emin Demir ◽  
Hakan Buyukhatipoglu
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Blood Glucose ◽  
Glucose Levels ◽  
Blood Glucose Levels

Treatment with N-acetylcysteine does not alter blood glucose levels and the oxidative stress status in diabetic rats

2013 ◽  
Vol 4 (1) ◽  
pp. 5 ◽  
Author(s):  
AndréValle de Bairros ◽  
Fernando de Freitas ◽  
Mirna Leal ◽  
Cinthia Mazzanti ◽  
AnaPaula Moreira ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Blood Glucose ◽  
Diabetic Rats ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Oxidative Stress Status

P1829Accumulation of advanced glycoxidation end products (AGEs) is associated with the severity of aortic stenosis in patients with concomitant type 2 diabetes

2019 ◽  
Vol 40 (Supplement_1) ◽  
Author(s):  
M Kopytek ◽  
M Zabczyk ◽  
A Undas ◽  
J Natorska
Keyword(s):  
Oxidative Stress ◽  
Blood Glucose ◽  
Aortic Valve ◽  
Aortic Stenosis ◽  
Aortic Valve Area ◽  
Science Center ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
End Products

Abstract Background Accumulation of advanced glycoxidation end products (AGEs) leads to chronic inflammation, glycation of proteins and tissue damage, particularly in patients with diabetes mellitus (DM). Purpose To evaluate whether increased valvular accumulation of AGEs in patients with aortic stenosis (AS) and concomitant diabetes (AS-DM) is associated with enhanced valvular inflammation, increased oxidative stress and AS progression. Methods We enrolled 50 patients (28 women), aged 67.2±7.9 years with severe AS (transvalvular gradients: PGmean=52.9±13.4 mmHg, PGmax=84.9±18.7 mmHg), including 30 subjects with type 2 DM (glycaemia 6.3±1.9 mM/l, glycated haemoglobin [HbA1c] 6.9±1.4%), who underwent aortic valve replacement. Valvular expression of AGEs, AGE receptors (RAGE), interleukin-6 (IL-6), prothrombin (FII), C-reactive protein (CRP), and reactive oxygen species (ROS) were evaluated ex vivo by single/double-immunostaining. The percentage of immuno-positive areas was analyzed for each valve. Results There were no differences with regard to age and AS severity between AS and AS-DM patients. Subjects with AS-DM had elevated valvular expression of both AGE and RAGE (41.3±15.2% and 17.6±7.1%, respectively) as compared to non-DM (9.4±3.9% and 3.4±2.4%, respectively; all p<0.001). In AS-DM patients AGE/RAGE showed co-expression in 51.5% of areas. The percentage of ROS-, CRP-, and IL-6-positive areas was elevated within AS-DM compared to AS valves (10.9±2.4%, 6±0.3%, and 5.7±1.4% vs 4.8±1.7%, 2.5±0.1%, and 1.6±0.9%, respectively; all p<0.05). Valvular expression of FII was elevated in AS-DM patients compared to those with AS (17.3±2.5% vs. 11±2%, p<0.05). In AS-DM patients the percentage of valvular AGE-positive areas correlated with HbA1c (r=0.44, p<0.05) and blood glucose levels (r=0.57, p<0,05). The amount of valvular RAGE correlated with HbA1c (r=0.63, p=0.009) but not with blood glucose levels. In AS-DM patients we found positive associations between the amount of valvular AGE and IL-6 with disease severity measured as aortic valve area (AVA; r=−0.83, p<0.0001; r=−0.64, p<0.001, respectively) and PGmax (r=0.54, p<0.05; r=0.52, p<0.05, respectively) but not with PGmean. There were no associations between PGmax/PGmean and HbA1c levels in the whole AS-DM group. However, patients with HbA1c >6.5% (n=19) or ≤6.5% (n=11) were characterized by higher PGmax and PGmean compared to those with HbA1c ≤6.5% (93.3±8.9 mmHg vs 67.5±7.2 mmHg and 63.6±6.5 mmHg vs 52±77 mmHg, respectively, all p<0.05). Conclusions Patients with AS-DM compared to those with AS demonstrate enhanced valvular AGEs accumulation and increased RAGE expression along with accelerated inflammation and oxidative stress. The accumulation of valvular AGE correlated with both hyperglycaemia and AS severity. Acknowledgement/Funding This work was supported by the grant from the Polish National Science Center (UMO-2015/19/B/NZ5/00647 to J.N).

Effects of Low Intensity Exercise Against Apoptosis and Oxidative Stress in Streptozotocin-induced Diabetic Rat Heart

2016 ◽  
Vol 125 (09) ◽  
pp. 583-591 ◽  
Author(s):  
M. Kanter ◽  
F. Aksu ◽  
M. Takir ◽  
O. Kostek ◽  
B. Kanter ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Blood Glucose ◽  
Cardiac Tissue ◽  
Diabetic Rat ◽  
Enzymatic Antioxidants ◽  
Citrate Buffer ◽  
Glucose Levels ◽  
Low Intensity ◽  
Blood Glucose Levels ◽  
Low Intensity Exercise

Abstract Background The aim of this study was to investigate the effects of low intensity exercise on heart of streptozotocin (STZ)-induced diabetic rats. Materials and Methods The rats were randomly divided into 3 experimental groups: A (control), B (diabetic untreated), and C (diabetic treated with low intensity exercise); each group contains 8 animals. B and C groups received STZ. Diabetes was induced in 2 groups by a single intraperitoneal (i.p) injection of STZ (40 mg/kg, freshly dissolved in 0,1 M citrate buffer, pH 4.2). 2 days after STZ treatment, diabetes in 2 experimental groups was confirmed by measuring blood glucose levels. Rats with blood glucose levels of 250 mg/dl or higher were considered to be diabetic. Animals in the exercise group were made to run the treadmill once a day for 4 consecutive weeks. Exercise started 3 days prior to STZ administration. Results After induction of diabetes, histological abnormalities were observed, including myofibrillar loss, vacuolization of cytoplasm and irregularity of myofibrils. These alterations were attenuated by low intensity exercise. Our data indicates a significant reduction of oxidative stress and apoptosis in cardiomyocytes after exercise. Treatment of diabetic animals with low intensity exercise, decreased the elevated tissue malondialdehyde (MDA) levels and increased the reduced activities of the enzymatic antioxidants superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) in cardiac tissue. Conclusion These findings suggest that low intensity exercise has a therapeutic protective effect in diabetes by decreasing oxidative stress and apoptosis, and by preservation of myocardial integrity.

scholarly journals The Effectiveness of Vitamin C Addition to Blood Glucose Levels of Hyperglycemic Mice with Glimepiride Treatment

2020 ◽  
Vol 6 (3) ◽  
pp. 149
Author(s):  
Indi Kamilia Fitri ◽  
Cholis Abrori ◽  
Dion Krismashogi Dharmawan
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Vitamin C ◽  
Pharmacological Therapy ◽  
Control Group ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Induction Group ◽  
Reduce Blood Glucose

In people with diabetes mellitus (DM) there is a decrease in basal vitamin C levels which is thought to be a result of oxidative stress in the condition of hyperglycemia that it needs to increase vitamin C as an antioxidant. Hyperglycemia in DM needs to be lowered by pharmacological therapy, named glimepirid so the purpose of this study is to determine the effectiveness of vitamin C addition to the reduction of KGD hyperglycemic mice with glimepirid treatment. This study is true experimental with a sample of 25 mice 20-30 grams and divided into five groups, first the control group (K0); STZ  induction group (K1); STZ induction group with glimepirid treatment (K2); STZ induction group with the treatment of vitamin C (K3); STZ induction group by treatment of a combination of glimepirid and vitamin C (K4). STZ is injected intraperitonially 150 mg / kgBB. All groups were measured for KGD 1 after induction of STZ and KGD 2 after treatment for fourteen days. The results of the STZ induction group with the treatment of a combination of glimepirid and vitamin C (K4) experienced a smaller and not significant decrease in BSL in the STZ induction group with a single glimepirid (K2) treatment. These results are thought to have an influence from interactions between drugs that cause one drug to not work optimally. The conclusion of this study is that administration of glimepirid, vitamin C, or both can reduce blood glucose levels in hyperglycemic mice with the greatest decrease occurring in the glimepirid group.

scholarly journals Ameolirative effects of garlic (Allium sativum L.,) in streptozotocin induced oxidative stress in diabetic brain of rat

2020 ◽  
Vol 11 (4) ◽  
pp. 7950-7957
Author(s):  
Pratap Reddy K ◽  
Bhaskar Nagilla ◽  
Varija K
Keyword(s):  
Oxidative Stress ◽  
Blood Glucose ◽  
Polyol Pathway ◽  
Diabetic Rats ◽  
Garlic Extract ◽  
Advanced Glycation ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Glycation End Products ◽  
End Products

The aim of the present study was to evaluate the protective effect of methanol garlic extract on the enzymes related with polyol pathway, advanced glycation end products, markers of oxidative stress and antioxidant status in brain of streptozotocin induced diabetic rats. Antioxidant capability of methanol extract of garlic was evaluated by 2,2-diphenyl-2-picrylhydrazyl hydrate radical and FOX (ferrous ion oxidation-xylenol orange) H2O2 scavenging test. Diabetes was induced by single i.p injection of STZ (32mg/kg per body Wt.,). Blood glucose levels and body wt, were measured on every 7th day over a period of 30 days. The diabetic rats treated with garlic extract at two doses 250mg/kg and 500mg/kg body wt., by oral administration. Diabetic rats showed significant increase in food and water intake, decrease in blood glucose levels, body weights, but could not show any recovery by garlic treatment. Garlic treatment significantly decreased aldose reductase (AR); sorbitol dehydrogenase (SD) and glutathione S-transferase (GST) enzyme activities. A decrease of malndialdehyde (MDA), Protein carbonyls (PC), Pentosidine advance oxidation protein products (AOPP), Advanced glycation end products (AGEs) was also observed. Additionally garlic administration produced a restoration of brain superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and reduced glutathione (GSH) in diabetic rats. Garlic treatment also reduced the free radical formation and progression of hyperglycemia induced diabetic complications by decreasing influx of glucose into the polyol pathway and increased activity of antioxidant enzymes. The current study reveals exerts, efficiently, an attenuating effect of methanol garlic extract exterted antihyperlgycemic, antioxidant and anti-glycating effects in a dose dependent manner in diabetic rats.

Sign in / Sign up

Export Citation Format

Share Document