scholarly journals STREPTOZOTOCIN-INDUCED OXIDATIVE STRESS IN DIABETIC RATS - A DEFENSIVE EFFECT OF PSYDRAX DICOCCOS

2018 ◽  
Vol 11 (11) ◽  
pp. 378
Author(s):  
Ravi Kumar V ◽  
Sailaja Rao P
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Enzymes ◽  
Blood Glucose ◽  
Methanolic Extract ◽  
Antioxidant Effect ◽  
Control Group ◽  
Standard Drug ◽  
Antihyperglycemic Activity ◽  
Glucose Levels ◽  
Blood Glucose Levels

Objective: The present study was aimed to evaluate the antihyperglycemic activity and in vivo antioxidant effect of methanolic extract of whole plant of Psydrax dicoccos (MEPD) belonging to the family Rubiaceae.Methods: MEPD was prepared by Soxhlet extraction. Wistar rats weighing (180–200 g) were divided into six groups (n=6), with three doses of 100 mg/kg, 200 mg/kg, and 400 mg/kg of extract. Metformin was used as a standard drug. Diabetes was induced by streptozotocin (STZ) (40–50 mg/kg, i.p) in control group. The animals were treated with different doses of extracts for 21 days, and on the 22nd day, the blood glucose levels along with antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), and lipid peroxidase (LPO) were determined.Results: The phytochemical screening of the extract showed the presence of carbohydrates, phenolics, flavonoids, glycosides, and tannins. The methanolic extract of MEPD at the dose of 200 mg/kg body weight showed a significant reduction in blood glucose levels (**p<0.001) with the value of 151.2 mg/dl on the 22nd day at 8 h. A promising antioxidant effect was also evident from the determination of antioxidant enzymes such as SOD, CAT, and LPO.Conclusion: The P. dicoccos extract revealed a potential effect of antihyperglycemic activity and combating nature on oxidative stress induced by STZ.

scholarly journals ARISTOLOCHIA BRACTEOLATA – ANTIHYPERGLYCEMIC AND ANTIHYPERLIPIDEMIC ACTIVITY IN DEXAMETHASONE-INDUCED DIABETIC RAT MODEL

2017 ◽  
Vol 10 (8) ◽  
pp. 75
Author(s):  
Ganga Rajum ◽  
Hema Sundar Reddy T ◽  
Hema Sundar Reddy T
Keyword(s):  
Blood Glucose ◽  
Methanolic Extract ◽  
Density Lipoprotein ◽  
Diabetic Rats ◽  
Diabetic Rat ◽  
Standard Drug ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Antihyperlipidemic Activity ◽  
Aristolochia Bracteolata

  Objective: The present study was aimed to evaluate antihyperglycemic and antihyperlipidemic activities of methanolic extract of Aristolochia bracteolata (MEAB) against dexamethasone-induced diabetic rat model.Methods: Methanolic extract was prepared by soxhlet extraction and was evaluated for antihyperglycemic and antihyperlipidemic activity using dexamethasone-induced model. The MEAB was administered orally at a dose of 200 and 400 mg/kg body weight glibenclamide was used as standard drug. On 0th and 11th day, blood was collected by retro-orbit plexus.Results: In this model blood glucose levels were determined on 0th and 11th days and MEAB significantly reduced the blood glucose levels in diabetic rats. The effect of MEAB on serum lipid profile such as total cholesterol (TC), triglycerides (TGs), low-density lipoprotein (LDL), very LDL (VLDL), and high-density lipoprotein (HDL) was also measured on the 11th day in the diabetic rats. Significant reduction in TC, TGs, LDL, and VLDL levels and improvement in HDL level were observed in diabetic rats.Conclusion: From the results, it was found that the MEAB possess antihyperglycemic and antihyperlipidemic activities.

scholarly journals The Effectiveness of Vitamin C Addition to Blood Glucose Levels of Hyperglycemic Mice with Glimepiride Treatment

2020 ◽  
Vol 6 (3) ◽  
pp. 149
Author(s):  
Indi Kamilia Fitri ◽  
Cholis Abrori ◽  
Dion Krismashogi Dharmawan
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Vitamin C ◽  
Pharmacological Therapy ◽  
Control Group ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Induction Group ◽  
Reduce Blood Glucose

In people with diabetes mellitus (DM) there is a decrease in basal vitamin C levels which is thought to be a result of oxidative stress in the condition of hyperglycemia that it needs to increase vitamin C as an antioxidant. Hyperglycemia in DM needs to be lowered by pharmacological therapy, named glimepirid so the purpose of this study is to determine the effectiveness of vitamin C addition to the reduction of KGD hyperglycemic mice with glimepirid treatment. This study is true experimental with a sample of 25 mice 20-30 grams and divided into five groups, first the control group (K0); STZ  induction group (K1); STZ induction group with glimepirid treatment (K2); STZ induction group with the treatment of vitamin C (K3); STZ induction group by treatment of a combination of glimepirid and vitamin C (K4). STZ is injected intraperitonially 150 mg / kgBB. All groups were measured for KGD 1 after induction of STZ and KGD 2 after treatment for fourteen days. The results of the STZ induction group with the treatment of a combination of glimepirid and vitamin C (K4) experienced a smaller and not significant decrease in BSL in the STZ induction group with a single glimepirid (K2) treatment. These results are thought to have an influence from interactions between drugs that cause one drug to not work optimally. The conclusion of this study is that administration of glimepirid, vitamin C, or both can reduce blood glucose levels in hyperglycemic mice with the greatest decrease occurring in the glimepirid group.

scholarly journals EFFECT OF TELMISARTAN ON BLOOD GLUCOSE LEVELS AND BLOOD LIPID LEVELS IN STREPTOZOTOCIN INDUCED DIABETIC RATS

2019 ◽  
Vol 12 (3) ◽  
pp. 1303-1308
Author(s):  
Siddamma Amoghimath ◽  
R. N. Suresha
Keyword(s):  
Blood Glucose ◽  
Lipid Profile ◽  
Blood Lipid ◽  
Diabetic Rats ◽  
Control Group ◽  
Albino Rats ◽  
Standard Drug ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Blood Lipid Levels

To evaluate the effect of telmisartan on blood glucose levels and blood lipid levels in streptozotocin induced diabetic rats. Eighteen Wistar albino rats weighing 150-200gms of either sex were randomly selected from the central animal facility, and divided into 3 groups. Diabetes was induced by injecting Streptozotocin intraperitonelly. The control group received 1% Gum acacia (oral), standard group received 0.5 mg/kg Glibenclamide (oral) and the test group received Telmisartan 7.2mg/kg body weight (oral) from 0-28 days respectively. Body weight of the individual rats were measured on the respective days before blood glucose estimation on 0, 1, 3, 7, 14, 21 & 28th day and fasting blood glucose was estimated by (ACCUCHECK) glucometer. Estimation of fasting lipid profile by lipid screening strips on 1st and 28th day. When compared to control the capillary blood glucose (CBG) levels in the Telmisartan group was less at all the intervals but comparable with that of standard drug Glibenclamide in Streptozotocin induced diabetic rats. Improved lipid profile was seen with the Telmisartan group when compared to control group in Streptozotocin induced diabetic rats. Hypoglycemic activity and improved lipid profile action was seen with Telmisartan group which is comparable to standard drug glibenclamide in streptozotocin induced diabetic albino rats.

scholarly journals Phytochemical and Hypoglycemic Evaluation of Alangium Salviifolium Root Extract

2011 ◽  
Vol 3 (2) ◽  
pp. 393-402 ◽  
Author(s):  
Ahad H. A. ◽  
Padmaja B. S ◽  
Yesupadam P ◽  
Guruprakash P ◽  
Sravanthi M ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Ethanolic Extract ◽  
Diabetic Rats ◽  
Control Group ◽  
Root Extract ◽  
Alcoholic Extract ◽  
Dependent Manner ◽  
Standard Drug ◽  
Glucose Levels ◽  
Blood Glucose Levels

.The main purpose of present study was to perform phytochemical screening and explore the anti-hyperglycaemic properties of Alangium salvifolium root extract in normal and alloxan induced diabetic rats. A. salvifolium root gave maximum extractive values of 6.4 % w/w with Ethanol and other parameters were within limits. The extract gave positive tests for phytosterols, triterpenes, flavonoids, carbohydrates and alkaloids. The ethanolic extract of A. salvifolium was found to be nearly as potent as tolbutamide in decreasing the blood glucose levels in normal fasting rats. In normal control group the percent reduction in blood glucose indicated that the extract is fast acting. The extract showed significant decrease in blood glucose, when compared with tolbutamide. The evaluated blood glucose levels in alloxan induced diabetic rats were significantly decreased up to 24th h compared to tolbutamide. A single oral administration of alcoholic extract at doses 100, 250 and 500 mg per kg produced a significant blood glucose reduction in a dose dependent manner in normal and diabetic rats. These data confirm the hypoglycaemic and anti-hyperglycaemic effect of alcoholic extract of A. salvifolium root in normal and diabetic rats respectively when compared with standard drug tolbutamide.Keywords: Alangium salvifolium; Ethanolic extract; Alloxan induced; Hypoglycaemic action.© 2011 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.doi:10.3329/jsr.v3i2.7047                J. Sci. Res. 3 (2), 393-402 (2011)

scholarly journals Changes in Oxidative Stress and Antioxidant Enzyme Activities in Streptozotocin-Induced Diabetes Mellitus in Rats: Role ofAlhagi maurorumExtracts

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
S. A. Sheweita ◽  
S. Mashaly ◽  
A. A. Newairy ◽  
H. M. Abdou ◽  
S. M. Eweda
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Ethanolic Extract ◽  
Insulin Level ◽  
Diabetic Rats ◽  
Control Group ◽  
Antioxidant Enzyme Activities ◽  
Glucose Levels ◽  
Blood Glucose Levels

Alhagi maurorum(camel thorn plant) is a promising medicinal plant due to the presence of flavonoids and phenolic compounds as major contents of its constituents. No previous study has been conducted before onA. maurorum extractsas an antioxidative stress and/or antidiabetic herb in STZ-induced DM in rats. Therefore, four groups of rats were allocated as control (C), STZ-induced DM (D), and STZ-induced DM supplemented with 300 mg/kg BW of either aqueous extract (WE) or ethanolic extract (EE) ofA. maurorum. The plasma levels of glucose, TG, TC, LDL-C and VLDL-C, MDA, and bilirubin and the activities of transaminases and GR were significantly increased in the diabetic group. Also, diabetic rats showed severe glucose intolerance and histopathological changes in their livers. In addition, levels of insulin, total proteins, GSH, and HDL-C and the activities of SOD, GPx, and GST were significantly decreased in the diabetic rats compared to those of the control group. The ingestion ofA. maurorumextracts lowered the blood glucose levels during the OGTT compared to the diabetic rats and restored all tested parameters to their normal levels with the exception of insulin level that could not be restored. It is concluded thatA. maurorumextracts decreased elevated blood glucose levels and hyperlipidemia and suppressed oxidative stress caused by diabetes mellitus in rats.

scholarly journals ANTI-HYPER LIPIDEMIC ACTIVITY OF METHANOLIC EXTRACT OF SMILAX WIGHTII A.DC. IN STREPTOZOTOCIN INDUCED MALE WISTAR ALBINO RATS

2014 ◽  
Vol 1 (2) ◽  
pp. 126-130
Author(s):  
Uma Maheswari P ◽  
Arumugasamy K ◽  
Shalimol A ◽  
Asha devi V ◽  
Nantha kumar R
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Plant Extract ◽  
Methanolic Extract ◽  
Fasting Blood Glucose ◽  
The Body ◽  
Lipid Lowering ◽  
Control Group ◽  
Glucose Levels ◽  
Blood Glucose Levels

Smilax wightii, an endemic medicinal plant is found in the shola forests at high altitudes in Nilgiri Biosphere Reserve, the Western Ghats, Southern India . The present study, was undertaken to find out the effect of methanolic extract of this plant on the body weight, fasting blood glucose levels and lipid profiles inall the streptozotocin (STZ) induced rats. The extract exerted a significant (P<0.05) effect in the body weight of the experimental animals when compared to the control group. Treatment with the extract and glibenclamide resulted in a significant (P<0.01) reduction in the fasting blood glucose levels in diabetic ratswhen compared to the normal. The lipid profile such as TC, TG, LDL, and VLDL contents in the serum registered a significant (P<0.01) hike and a decline in the HDL contents in diabetic control group, which were retrieved to near normalcy in the plant extract treated groups. The effect produced by this plant extract wascomparable with that of glibenclamide. The decreased fasting blood glucose levels and lipid lowering properties clearly showed the anti-hyperlipidemic effect of S.wightii.

scholarly journals A study of hypoglycemic effect of Caesalpinia bonduc extract on alloxan induced diabetic albino rats

2017 ◽  
Vol 6 (9) ◽  
pp. 2153
Author(s):  
Reshma Nadaf
Keyword(s):  
Blood Glucose ◽  
Diabetic Control ◽  
Hypoglycemic Effect ◽  
Control Group ◽  
Albino Rats ◽  
Alcoholic Extract ◽  
Test Drug ◽  
Standard Drug ◽  
Glucose Levels ◽  
Blood Glucose Levels

Background: The objective of the study was to scientifically investigate the oral hypoglycemic activity of Caesalpinia bonduc on Alloxan induced diabetic albino rats. To compare the hypoglycemic effect of Caesalpinia bonduc with that of the standard drug Glibenclamide used in the treatment of diabetes mellitus.Methods: Adult healthy albino rats of wister strain of either sex weighing 150-200gms were included in the study. The animals were divided into 4 groups namely control, diabetic control, standard and test groups with 6 animals in each group. Diabetes was chemically induced using alloxan to produce hyperglycemia in rats. Standard drug Glibenclamide suspended in gum acacia was administered for standard group. Test drug Caesalpinia bonduc was administered for test group. Morning around 9 a.m. blood glucose levels were recorded on 1st, 3rd, 7th, 14th, 21st, and 28th days.Results: The control group of rats showed no variation. The diabetic control rats showed consistent hyperglycemia. Comparing the test drug Caesalpinia bonduc to the standard drug Glibenclamide, the test drug was 1.38 times more efficacious than the standard.Conclusions: The alcoholic extract of Caesalpinia bonduc (seeds) has shown more anti diabetic activity by lowering the blood glucose levels in diabetic rats significantly. These findings suggest that hypoglycemic potential of the test compound Caesalpinia bonduc is promising and found to be more significant than the standard compound.

PENGARUH PEMBERIAN FRAKSI ETILASETAT KULIT UBI JALAR UNGU TERHADAP KADAR MALONDIALDEHID (MDA) SERUM MENCIT PUTIH JANTAN HIPERGLIKEMIA

2018 ◽  
Vol 8 (2) ◽  
pp. 144
Author(s):  
Ria Afrianti
Keyword(s):  
Blood Glucose ◽  
Statistical Analysis ◽  
Sweet Potato ◽  
Ethyl Acetate Fraction ◽  
Serum Levels ◽  
Negative Control ◽  
Control Group ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Purple Sweet Potato

This study aims to determine the effect giving of ethylacetate fraction of leather  purple sweet potato (Ipomoea batatas (L.) Lam, on levels of malondialdehyde (MDA) serum in mice hyperglicemia were induced with streptozocin dose of 50 mg/kgBW. Mice were divided into 5 groups, each group consisting of 3 tails, group I is a negative control, group II is a positive control, group III,IV and V is given ethylacetate fraction a dose of 100 mg/kgBW, 300 mg/kgBW, and 600 mg/kgBW. Ethyl Acetate Fraction leather purple sweet potato given orally for 15 days after the animal is declared hyperglicemia and measurement of blood glucose levels on 5, 10, and 15 day after giving test preparation in animal experiments. On the 16 day throughout the mice were taken serum levels measured malondialdehid. The statistical analysis results showed that giving of ethyl acetate fraction of leather purple sweet potato at a dose of 100 mg/kgBW, 300 mg/kgBW, and 600 mg/kgBW can lower blood glucose levels in mice hyperglycemia significantly (p<0.05). Malondialdehid levels on average in each group is 1.35 nmol/ml, 3.00 nmol/ml, 2.72 nmol/ml, 2.20 nmol/ml and 2.61 nmol/ml, the results of statistical analysis showed a decrease in melondialdehid serum levels were significantly (p<0.05), where a dose of 300 mg/kgBW is an effective dose for lowering blood glucose levels followed by decreased levels of malondialdehid which give effect approaching negative control.

Efek Ekstrak Etanol Kulit Petai (Parkia speciosa Hassk) Terhadap Penurunan Kadar Glukosa Darah Mencit Jantan

2018 ◽  
Vol 3 (1) ◽  
pp. 1
Author(s):  
Verawaty Verawaty ◽  
Dhea Claudia Novel
Keyword(s):  
Blood Glucose ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Male Mice ◽  
Glucose Levels ◽  
The Third ◽  
Blood Glucose Levels

<p>Penelitian ini bertujuan untuk melihat pengaruh pemberian ekstrak etanol kulit petai (Parkia speciosa Hassk) terhadap penurunan kadar glukosa darah mencit jantan yang diinduksi aloksan. Hewan percobaan dibagi atas 5 kelompok diantaranya kelompok kontrol negatif, kelompok kontrol positif,dosis I (280 mg/kgBB mencit), dosis II (560 mg/kg BB mencit), dosis III (840 mg/kg BB mencit). Penelitian dilakukan selama 21 hari. Persentase penurunan kadar glukosa darah mencit jantan setelah diberikan ekstrak etanol kulit petai pada hari ke-21 adalah dosis I (77,52 %) lebih besar dibandingkan dengan dosis II (69,5 %) dan dosis III (73,37 %). Data yang diperoleh dianalisis dengan uji Two Way Anova dengan program SPSS 17. Hasil penelitian ini menunjukkan bahwa pemberian ekstrak etanol kulit petai untuk tiga variasi dosis menyatakan perbedaan yang bermakna secara statistik terhadap penurunan kadar glukosa darah mencit jantan.</p><p><em>Petai (Parkia speciosa Hassk) has a compound β-sitosterol and stigmasterol that have efficacy to decreased blood glucose levels. This study aimed to determine the effect of ethanol extract of petai peel for decrease blood glucose levels of male mice induced by alloxan. Experimental animals were divided into 5 groups including negative control group, positive control group, the first dose (280 mg/kg in mice), the second dose (560 mg/kg in mice), the third dose (840 mg/kg in mice). The study was conducted for 21 days. After 21 days, the result found that the percentage of blood glucose levels after the male mice given the ethanol extract of petai peel was, the first dose (77.52%) biger than the second dose (69.5%) and the third dose (73.37%). The data obtained were analyzed by Two Way ANOVA using SPSS 17. The results showed that have signicantly difference between three dose variation of ethanol extract of petai peel in blood glucose levels.</em></p>

Influence of Lactobacillus brevis 15 and Lactobacillus plantarum 13 on blood glucose and body weight in rats after high-fructose diet

2015 ◽  
Vol 6 (4) ◽  
pp. 505-512 ◽  
Author(s):  
M. Yakovlieva ◽  
T. Tacheva ◽  
S. Mihaylova ◽  
R. Tropcheva ◽  
K. Trifonova ◽  
...  
Keyword(s):  
Body Weight ◽  
Lactic Acid ◽  
Blood Glucose ◽  
Lactic Acid Bacteria ◽  
Lactobacillus Plantarum ◽  
Lactobacillus Brevis ◽  
Control Group ◽  
Standard Diet ◽  
Glucose Levels ◽  
Blood Glucose Levels

In recent years, many authors have investigated the possible antidiabetic effect of lactic acid bacteria. Lactobacillus species constitute a major part of the lactic acid bacteria group and have been found to exhibit beneficial effects on the development of diabetes and its complications. In the current study, we investigated the effects of newly characterised Bulgarian Lactobacillus strains, Lactobacillus brevis 15 and Lactobacillus plantarum 13, on blood glucose levels and body weight of rats fed a fructose-enriched diet. An experiment was conducted over a period of 8 weeks with 24 2-month-old Wistar rats randomly assigned to receive a standard diet (Con, control group), fructose-enriched diet (Fr group), standard diet with probiotics given twice a week (Pro group), and fructose-enriched diet with probiotics given twice a week (Pro+Fr group). At the end of the experimental period, a statistically significant increase in body weight was observed in all experimental groups (P<0.0001). The highest rise was seen in the fructose group (Fr, 169±19 g), followed by the Pro+Fr group (153±15 g), Pro group (149±13 g), and Con group (141±5 g). Moreover, the final blood glucose levels had risen significantly in the groups receiving fructose either without (Fr; P<0.0001) or with lactobacilli (Pro+Fr; P=0.002), while the rise was insignificant in the group of rats given probiotic supplementation only (Pro, P=0.071) and inexistent in the Con group (P=0.999). The highest elevation of blood glucose levels was observed in the Fr group (3.18 mmol/l), followed by the Pro+Fr group (2.00 mmol/l) whereas the Pro group showed the lowest levels (0.60 mmol/l). The results of our study suggest that the newly characterised Bulgarian Lactobacillus strains, L. brevis 15 and L. plantarum 13, could be considered as possible probiotics and might be able to prevent some metabolic disturbances.

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