scholarly journals Detection of pathogenic bacteria associated with earphones used by students of Stamford University Bangladesh

2020 ◽  
Vol 10 (1) ◽  
pp. 1-4
Author(s):  
Omor Ahmed Chowdhury ◽  
Md Raihan Ahmed ◽  
Md Raihan Dipu ◽  
Md Aftab Uddin
Keyword(s):  
Pathogenic Bacteria ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Bacterial Isolates ◽  
Biochemical Tests ◽  
Disk Diffusion Method ◽  
Antibiotic Resistant ◽  
Potential Source ◽  
Different Types ◽  
Staphylococcus Spp

The use of earphones has increased in recent times throughout the world especially among the different level of students such as school, college or university who have a higher tendency of sharing these among them. Unlike airline headsets, headphones and stethoscope ear-pieces, ear phones are often shared by multiple users and can be a potential medium for transmission of pathogens, which can give rise to various ear related infections. The objective of this study was to detect the pathogenic bacteria from the ear-phones used by the students of Stamford University Bangladesh. A total of 16 ear-phone swabs were collected by sterile cotton swabs. The swabs were inoculated onto blood agar and incubated aerobically overnight at 37oC. Microscopic observation and standard biochemical tests were performed to confirm the identification of all the bacterial isolates. Six presumptively identified Staphylococcus spp. (38%) were tested against six different types of antibiotics following Kirby-Bauer disk diffusion method. Isolates were found to be 84% resistant against Cotrimoxazole and demonstrated 100% sensitivity to Vancomycin and Ciprorofloxacin. The findings of this study suggest the users to disinfect their respective ear phones and not to exchange them as they may act as a potential source to transfer pathogenic and antibiotic resistant bacteria among the ear phone users. Stamford Journal of Microbiology, Vol.10 (1) 2020: 1-4

scholarly journals Antibiotic Resistance of Diverse Bacteria from Aquaculture in Borneo

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
M. M. Kathleen ◽  
L. Samuel ◽  
C. Felecia ◽  
E. L. Reagan ◽  
A. Kasing ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Bacterial Isolates ◽  
Disk Diffusion Method ◽  
Antibiotic Resistant ◽  
Cultured Fish ◽  
Aquaculture Industry ◽  
Mar Index ◽  
Malaysian Borneo

The administration of antimicrobials in aquaculture provides a selective pressure creating a reservoir of multiple resistant bacteria in the cultured fish and shrimps as well as the aquaculture environment. The objective of this study was to determine the extent of antibiotic resistance in aquaculture products and aquaculture’s surrounding environment in Sarawak, Malaysian Borneo. Ninety-four identified bacterial isolates constituted of 17 genera were isolated from sediment, water, and cultured organisms (fish and shrimp) in selected aquaculture farms. These isolates were tested for their antibiotic resistance against 22 antibiotics from several groups using the disk diffusion method. The results show that the highest resistance was observed towards streptomycin (85%,n=20), while the lowest resistance was towards gentamicin (1.1%,n=90). The multiple antibiotic resistant (MAR) index of the isolates tested ranged between 0 and 0.63. It was suggested that isolates with MAR index > 0.2 were recovered from sources with high risk of antibiotic resistant contamination. This study revealed low level of antibiotic resistance in the aquaculture bacterial isolates except for streptomycin and ampicillin (>50% resistance,n=94) which have been used in the aquaculture industry for several decades. Antibiotic resistant patterns should be continuously monitored to predict the emergence and widespread of MAR. Effective action is needed to keep the new resistance from further developing and spreading.

scholarly journals Otodectic and bacterial etiology of feline otitis externa in Tripoli, Libya

2021 ◽  
Vol 10 (4) ◽  
pp. 377-383
Author(s):  
Murad A. Hiblu ◽  
Omar M. Ellraiss ◽  
Elfurgani S. Karim ◽  
Rabia A. Elmishri ◽  
Enass M. Duro ◽  
...  
Keyword(s):  
Antimicrobial Susceptibility ◽  
Otitis Externa ◽  
Diffusion Method ◽  
Bacterial Isolates ◽  
Disk Diffusion Method ◽  
Parasite Detection ◽  
Common Etiology ◽  
Etiological Agents ◽  
Staphylococcus Spp ◽  
Otodectes Cynotis

Background: Feline otitis externa is a dermatological disorder with a multifactorial complex etiology.Aim: This study aimed to investigate the prevalence of different etiological agents, particularly the parasitic and bacterial, responsible for the cases of feline otitis externa in Tripoli, Libya, and to assess the antimicrobial susceptibility of the bacterial isolates from those cases.Methods: Cerumen and otic discharges of the suspected cats were collected for parasite detection and bacterial culture. Kirby–Bauer’s disk diffusion method was used for antimicrobial susceptibility testing.Results: The results showed that otodectic mites and bacterial causes were equally the most prevalent in those cases, with a prevalence of 47.1% each. Otodectes cynotis infestation was more frequently bilateral and severe. Staphylococcus spp. were the most prevalent among bacterial causes (75%), followed by Proteus spp. (16.6%) and Pseudomonas spp. (8.4%). Norfloxacin and gentamicin were the most effective antimicrobials against bacterial isolates, as they were effective against 83.3% and 70.8% of isolates, respectively.Conclusion: Otodectes cynotis infestation and staphylococcal infections constituted the most common etiology of feline otitis externa in Tripoli, Libya, and norfloxacin represented a cogent antibacterial for the treatment of otitis externa.

scholarly journals Molecular Detection and Antibiotyping of Multidrug-Resistant Salmonella Isolated from Houseflies in a Fish Market

Pathogens ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 191 ◽  
Author(s):  
Sobur ◽  
Hasan ◽  
Haque ◽  
Mridul ◽  
Noreddin ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Multidrug Resistant ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Salmonella Spp ◽  
Disk Diffusion Method ◽  
Antibiotic Resistant ◽  
Fish Market ◽  
Pcr Method ◽  
High Level

Houseflies (Musca domestica) are well-known mechanical vectors for spreading multidrug-resistant bacteria. Fish sold in open markets are exposed to houseflies. The present study investigated the prevalence and antibiotypes of multidrug-resistant (MDR) Salmonella spp. in houseflies captured from a fish market. Direct interviews with fish vendors and consumers were also performed to draw their perceptions about the role of flies in spreading antibiotic-resistant bacteria. A total of 60 houseflies were captured from a local fish market in Bangladesh. The presence of Salmonella spp. was confirmed using PCR method. Antibiogram was determined by the disk diffusion method, followed by the detection of tetA, tetB, and qnrA resistance genes by PCR. From the interview, it was found that most of the consumers and vendors were not aware of antibiotic resistance, but reported that flies can carry pathogens. Salmonella spp. were identified from the surface of 34 (56.7%) houseflies, of which 31 (91.2%) were found to be MDR. This study revealed 25 antibiotypes among the isolated Salmonella spp. All tested isolates were found to be resistant to tetracycline. tetA and tetB were detected in 100% and 47.1% of the isolates, respectively. Among the 10 isolates phenotypically found resistant to ciprofloxacin, six (60%) were found to be positive for qnrA gene. As far as we know, this is the first study from Bangladesh to report and describe the molecular detection of multidrug-resistant Salmonella spp. in houseflies in a fish market facility. The occurrence of a high level of MDR Salmonella in houseflies in the fish market is of great public health concerns.

Antibiotic Resistance of Enterobacteriaceae Isolated from Fresh Fruits and Vegetables and Characterization of their AmpC β-Lactamases

2019 ◽  
Vol 82 (11) ◽  
pp. 1857-1863 ◽  
Author(s):  
ZAHRA S. AL-KHAROUSI ◽  
NEJIB GUIZANI ◽  
ABDULLAH M. AL-SADI ◽  
ISMAIL M. AL-BULUSHI
Keyword(s):  
Antibiotic Resistance ◽  
Fruits And Vegetables ◽  
Multidrug Resistant ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Disk Diffusion Method ◽  
Antibiotic Resistant ◽  
Amoxicillin Clavulanic Acid ◽  
Clinically Significant ◽  
Enterobacter Asburiae

ABSTRACT Enterobacteria may gain antibiotic resistance and be potent pathogens wherever they are present, including in fresh fruits and vegetables. This study tested the antibiotic resistance of enterobacteria isolated from 13 types of local and imported fresh fruits and vegetables (n = 105), using the standard Kirby-Bauer disk diffusion method. Phenotypic and genotypic characterizations of AmpC β-lactamases were determined in cefoxitin-resistant isolates. Ten percent of the enterobacteria tested (n = 88) were pansusceptible, 74% were resistant to at least one antibiotic, and 16% were multidrug resistant. Enterobacteria isolates showed the highest antibiotic resistance against ampicillin (66%), cephalothin (57%), amoxicillin–clavulanic acid (33%), cefoxitin (31%), tetracycline (9%), nalidixic acid (7%), trimethoprim (6%), and kanamycin (5%). Three isolates showed intermediate resistance to the clinically important antibiotic imipenem. Escherichia coli isolated from lettuce exhibited multidrug resistance against five antibiotics. Fifteen isolates were confirmed to have AmpC β-lactamase, using the inhibitor-based test and the antagonism test; the latter test confirmed that the enzyme was an inducible type. Four types of ampC β-lactamase genes (CIT, EBC, FOX, and MOX) were detected in eight isolates: four Enterobacter cloacae isolates and one isolate each of Citrobacter freundii, Enterobacter asburiae, Enterobacter hormaechei, and Enterobacter ludwigii. It was concluded that fresh fruits and vegetables might play a role as a source or vehicle for transferring antibiotic-resistant bacteria that might spread to other countries through exportation. The clinically significant AmpC β-lactamase was rarely documented in the literature on bacteria isolated from fruits and vegetables, and to our knowledge, this is the first report on the detection of an inducible type in such commodities.

scholarly journals Plasmid-Mediated Antibiotic-Resistant Pattern of Lactobacillus spp. Isolated From Dairy Products

2021 ◽  
Vol 8 (1) ◽  
pp. 1-4
Author(s):  
Fatemeh Shafiei Seifabadi ◽  
Majid Baserisalehi
Keyword(s):  
Antibiotic Resistance ◽  
Dairy Products ◽  
Pathogenic Bacteria ◽  
Dairy Product ◽  
Antibiotic Resistance Genes ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Antibiotic Resistant ◽  
Resistant Genes ◽  
Susceptibility Assay

Background: Microorganisms have potent activity for transferring antibiotic-resistant genes with either chromosomally- or plasmid-mediated characteristics. The purpose of this study was to isolate Lactobacillus from different commercial products and evaluate their potential in antibiotic-resistant development. Chromosomally-or plasmid-mediated resistant genes were investigated as well. Methods: In total, Lactobacillus strains were isolated from 20 commercial dairy product samples such as cheese and yoghurt. The isolates were phenotypic and molecularly identified and their antibiotic-resistant properties were assessed by the disk-diffusion method. Finally, the plasmid-mediated antibiotic resistant characters of the isolates were evaluated by plasmid curing via evaluated temperatures and acridine orange methods. Results: Five strains Lactobacillus paracasei, L. rhamnosus, L. casei, L. plantarum, and L. fermentum were isolated different products. The results of the antibiotic susceptibility assay indicated that all strains were susceptible to amoxicillin and imipenem and resistant to ciprofloxacin and vancomycin. Furthermore, different responses were observed among the isolates against streptomycin and gentamicin. The evaluation of plasmid-mediated antibiotic resistance in the isolates revealed that streptomycin and gentamicin-resistant characters were of plasmid-mediated type in L. rhamnosus and L. plantarum strains. Conclusions: In general, our finding demonstrated that some commercial Lactobacillus strains harboured antibiotic-resistant genes. These genes can be located either in chromosome or plasmid group. Hence, the frequency of antibiotic-resistant pathogenic bacteria might be increased after consuming some dairy products because of the horizontal transfer of antibiotic-resistance genes among the bacteria.

scholarly journals Identification and Antibiotic Resistance Profile of Salmonella spp. and Citrobacter spp. Isolated from Street-Vended Beverages

2021 ◽  
Vol 29 (1) ◽  
Author(s):  
Siti Shahara Zulfakar ◽  
Noraziah Mohamad Zin ◽  
Siti Nur Shafika Mat Zalami ◽  
Nur Syakirah Mohd Nawawee
Keyword(s):  
Antibiotic Resistance ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Antibiotic Resistant Bacteria ◽  
Salmonella Spp ◽  
Disk Diffusion Method ◽  
Antibiotic Resistant ◽  
Resistance Profile ◽  
Antibiotic Resistance Profile ◽  
Mar Index

The risk of foodborne diseases as well as the dissemination of antibiotic resistant bacteria increases with the consumption of street-vended food and beverages. This study investigated the prevalence of Salmonella spp. and Citrobacter spp. in street-vended beverages sold in Chow Kit, Kuala Lumpur, Malaysia. The Kirby-Bauer disk diffusion method was used to identify the antibiotic resistance profile of Salmonella spp. and Citrobacter spp. isolates towards 11 selected antibiotics. Six beverage samples were found positive for presumptive Salmonella spp. and Citrobacter spp. Upon confirmation via Microgen kit and PCR biochemical testing methods, only one isolate was confirmed to be Salmonella enterica serovar Derby while the other isolates were identified as Citrobacter spp. (n= 12; 2 isolates from each positive beverage sample). The antibiogram test showed that 58.3%, 16.7%, and 8.3% of the strains tested were resistance to tetracycline, cephalexin, and ampicillin respectively, while all isolates were fully resistant toward penicillin and erythromycin. The isolate with the highest MAR index (0.45) was S231, with resistance to five of the tested antibiotics (penicillin, erythromycin, tetracycline, cephalexin, and ampicillin). Seven isolates had a MAR index of 0.27 and were resistant to three antibiotics, while the remaining four isolates had the lowest MAR index (0.18) and were resistant to only two antibiotics. This study shows that street-vended beverages have a high risk of spreading antibiotic-resistant bacteria to the public and that Citrobacter spp. should be considered as emerging multidrug-resistant bacteria in the food production system.

scholarly journals Prevalence and Antibiotic Susceptibility of Salmonella from Chicken Eggs in Naogaon District of Bangladesh

2019 ◽  
pp. 1-6 ◽  
Author(s):  
Md. Saroat Hossain ◽  
K. M. Mozaffor Hossain ◽  
Md. Mahbubul Alam Sarker ◽  
Sm. Ahasanul Hamid
Keyword(s):  
Antibiotic Susceptibility ◽  
Multidrug Resistant ◽  
Diffusion Method ◽  
Biochemical Tests ◽  
Disk Diffusion Method ◽  
Antibiotic Resistant ◽  
Potential Reservoir ◽  
Antibiotic Susceptibility Patterns ◽  
Zoonotic Bacteria ◽  
Chicken Eggs

Salmonella is one of the most common zoonotic bacteria that cause foodborne illness in humans. An investigation was conducted to determine the prevalence and antibiotic susceptibility patterns of Salmonella isolates from chicken eggs in the Naogaon district, Bangladesh. Salmonella was isolated from cultures on different selective-differential media and further identified by biochemical tests. Antibiogram study was done by the disk diffusion method. The overall prevalence of Salmonella was recorded as 7.78%, whereas 5.56% was on eggshell surfaces and 2.22% was in egg contents. The Salmonella prevalence was 8.33%, 13.33% and 1.67% in chicken eggs from layer farms, whole sellers and retailers, respectively. Salmonella isolates were found 50.0% to 85.71% sensitive to chloramphenicol, gentamycin, ciprofloxacin, and ceftriaxone. Resistance against gentamycin, chloramphenicol, and ampicillin was found significant ranging from 21.43% to 71.42%. The highest resistance was found in amoxicillin (92.86%). The present study proposes that chicken eggs are a potential reservoir of multidrug-resistant Salmonella. Antibiotic-resistant Salmonella will pose a problem to treat Salmonella infection in humans. Thus, the aim of this study is to assess the risk of Salmonella resistance in chicken eggs.

scholarly journals Isolation of Escherichia coli, Klebsiella spp. and Staphylococcus spp. from Bovine Mastitic Milk in Nuwera Eliya District of Sri Lanka and their Sensitivity to Commonly Used Antibiotics

2018 ◽  
pp. 1-13
Author(s):  
T. S. P. J. Jayaweera ◽  
J. L. P. C. Randika ◽  
H. G. C. L. Gamage ◽  
N. N. Udawatta ◽  
W. U. N. T. S. Ellepola ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Sri Lanka ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Disk Diffusion Method ◽  
E Coli ◽  
Milk Samples ◽  
Staphylococcus Spp ◽  
Klebsiella Spp ◽  
Clinical Cases

Aims: Mastitis is one of the very important and most common diseases among dairy cattle globally which leads to severe economical losses in the dairy industry. For the sustainability of the dairy sector it is critical that efficient, economically feasible treatment regime is available for clinical cases of mastitis as a part of the control program with minimum risk for residues in milk. Antimicrobials are the most common drugs of choice for controlling and preventing this devastating condition. But the frequent use of antibiotics leads to the development of resistant bacteria which could have an adverse effect on human health as well. To mitigate this destructive constraint in the industry, identifying the etiology and their susceptibilities to remedial measures are of paramount importance. Hence this study was aimed at isolating and identifying the common bacterial etiology Escherichia coli, Klebsiella spp. and Staphylococcus spp. of mastitis and evaluating the antimicrobial susceptibility of the isolates in order to develop mastitis control strategies in the area. Study Design: Milk samples were collected from mastitic cows in different stages including subclinical and clinical cases based on the results of California Mastitis Test Place and Duration of Study: Samples were collected from dairy farms in Nuwera Eliya District, Sri Lanka and Laboratory investigations were carried out in the Laboratory of Livestock Production, Faculty of Agricultural Sciences, Sabaragamuwa University of Sri Lanka, Between Aug. 2017 and Nov. 2017. Methodology: E.coli, Klebsiella spp. and Staphylococcus spp. were isolated from 31 milk samples and susceptibility to commonly used antibiotics (Trimethoprim, Oxytetracycline, Chloramphenicol, Cephalexin, Enrofloxacin and Ciprofloxacin) was determined by Kirby Bauer disk diffusion method. Results: The study revealed that the most common isolate was the Klebsiella spp. and it is 54.8% and other two organisms Staphylococcus spp. had 51.6% and Escherichia coli 41.9%. Of all isolated pathogen, 97.1% exhibited resistant to Cephalexin and it was the highest while lowest resistance was to Chloramphenicol (31.4%). Among the other antibiotics, 54.3% of total isolates showed resistance to Trimethoprim followed by 42.9% to Oxytetracycline and Enrofloxacin, 34.3% was resistant to Ciprofloxacin. Resistance to at least one antibiotic was observed for the isolated microorganisms. All the three isolated pathogens are more resistant to Cephalexin. Both E. coli and Klebsiella spp. show 100% resistance to Cephalexin while Staphylococci had 92.9% resistance. This further revealed that E. coli (10%) and Klebsiella spp. (27.3%) are showing the least resistance to Chloramphenicol, None of Staphylococcus spp. (0%) isolated show resistance to Enrofloxacin. Conclusion: Most common organisms isolated were Klebsiella spp. followed by Staphylococcus spp., E. coli and there is a resistance of isolated organisms to some commonly used antibiotics.

scholarly journals Potential of Sambiloto (Andrographis paniculata Nees) Extract against Salmonella Pullorum

2022 ◽  
Author(s):  
Sri Hidanah ◽  
Emy Koestanti Sabdoningrum ◽  
Soeharsono . ◽  
Ayu Andira ◽  
Noor Amina Varhana
Keyword(s):  
Pathogenic Bacteria ◽  
Bacterial Resistance ◽  
Chemical Constituents ◽  
Disk Diffusion ◽  
Diffusion Method ◽  
Economic Losses ◽  
Biochemical Tests ◽  
Disk Diffusion Method ◽  
Salmonella Pullorum ◽  
Major Chemical

Background: Salmonella Pullorum are pathogenic bacteria that causes salmonellosis and causes heavy economic losses in the poultry industry and are zoonotic. Treatment of diseases caused by bacteria generally use antibiotics, but excessive administration of antibiotics causes bacterial resistance and residues in livestock. Major chemical constituents of Sambiloto are andrographolide and flavonoids. Andrographolide has antibacterial effect in addition to being antitoxic, anticancer, anti-inflammatory and antiallergic. Methods: The research was conducted by isolating and identifying Salmonella Pullorum on SSA media and a series of biochemical tests (TSIA, SIM, SCA, urea media and sugar test), manufacturing sambiloto extract, testing the sensitivity of several antibiotics using the disk diffusion method and testing the activation of sambiloto extract against Salmonella Pullorum using the disk diffusion and dilution methods. Result: The result show that sambiloto had antibacterial activity because it contained andrographolide, flavonoids, saponins, alkaloids and tannins and the lowest extract dose that effectively killed Salmonella Pullorum is concentrations of 20%.

scholarly journals Bacterial profiles and their antimicrobial susceptibility pattern of Isolates from inanimate hospital environments at Tikur Anbessa Specialized Teaching Hospital, Addis Ababa, Ethiopia

2020 ◽  
Author(s):  
Shemse Sebre ◽  
Woldaregay Erku ◽  
Aminu Seman ◽  
Tewachw Awoke ◽  
Zelalem Desalegn ◽  
...  
Keyword(s):  
Antimicrobial Susceptibility ◽  
Medical Equipment ◽  
Pathogenic Bacteria ◽  
Addis Ababa ◽  
Hospital Environment ◽  
Diffusion Method ◽  
Susceptibility Pattern ◽  
Biochemical Tests ◽  
Disk Diffusion Method ◽  
Antimicrobial Susceptibility Pattern

AbstractMicrobial contamination of hospital environment plays an important role in the spread of health care-associated infections (HCAIs). This study was conducted to determine bacterial contamination, bacterial profiles and antimicrobial susceptibility pattern of bacterial isolates from environmental surfaces and medical equipment. A cross-sectional study was conducted at Tikur Anbessa Specialized Hospital (TASH) from June to September, 2018. A total of 164 inanimate surfaces located at intensive care units (ICUs) and operation theaters (OTs) were swabbed. All isolates were identified by using routine bacterial culture, Gram staining and a panel of biochemical tests. For each identified bacteria, antibiogram profiles were determined by the Kirby Bauer disk diffusion method according to the guidelines of the Clinical and Laboratory Standards Institute (CLSI). Out of the 164 swabbed samples, 141 (86%) were positive for bacterial growth. The predominant bacteria identified from OTs and ICUs were S. aureus (23% vs 11.5%), Acinetobacter spp (3.8% vs 17.5%) and Coagulase negative Staphylococcus (CONS) (12.6% vs 2.7%) respectively. Linens were the most contaminated materials among items studied at the hospital (14.8%). The proportions of resistance among Gram-positive bacteria (GPB) were high for penicillin (92.8%), cefoxitin (83.5%) and erythromycin (54.1%). However, the most effective antibiotics were clindamycin with only 10.4% and 16.5% resistance rates, respectively. The antimicrobial susceptibility profiles of Gram-negative bacteria (GNB) revealed that the most effective antibiotics were amikacin, ciprofloxacin, and gentamicin with resistance rate of 25%, 37.5%, and 46.3%, respectively. However, the highest resistance was recorded against ampicillin (97.5%), ceftazidime (91.3%), ceftriaxone (91.3%) and aztreonam (90%). The inanimate surfaces near immediate patient environment and commonly touched medical equipment within OTs and ICUs are reservoirs of potential pathogenic bacteria that could predispose critically ill patients to acquire HCAIs. The proportions of antimicrobial resistance profile of the isolates are much higher from studied clean inanimate environments.

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