scholarly journals Notes on Lasioptera rubi (Schrank) (Diptera: Cecidomyiidae) and its larval parasitoids (Hymenoptera) on raspberries in Russia

2019 ◽  
Vol 27 (1) ◽  
pp. 15-22
Author(s):  
Ekaterina Yegorenkova ◽  
Zoya Yefremova
Keyword(s):  
Rubus Idaeus ◽  
Host Larva ◽  
Larval Parasitoids ◽  
Hymenopteran Parasitoids ◽  
First Time

In this paper, larva and pupa of Lasioptera rubi (Schrank) infected by hymenopteran parasitoids are illustrated. For the first time it is found that Platygaster pelias Walker is a solitary parasite and winters at the embryonal stage inside host larva of L. rubi reared from galls on Rubus idaeus stems in Russia. Lasioptera rubi, Torymus eadyi Graham & Gij swijt and Platygaster pelias Walker are recorded from Russia for the first time.

scholarly journals Combining QTL Mapping and Gene Expression Analysis to Elucidate the Genetic Control of ‘Crumbly’ Fruit in Red Raspberry (Rubus idaeus L.)

Agronomy ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 794
Author(s):  
Luca M. Scolari ◽  
Robert D. Hancock ◽  
Pete E. Hedley ◽  
Jenny Morris ◽  
Kay Smith ◽  
...  
Keyword(s):  
Genetic Control ◽  
Developmental Disorder ◽  
Molecular Mechanisms ◽  
Developmental Stages ◽  
Microarray Experiment ◽  
Rubus Idaeus ◽  
Red Raspberry ◽  
Genotype By Sequencing ◽  
First Time ◽  
Selection Of

‘Crumbly’ fruit is a developmental disorder in raspberry that results in malformed and unsaleable fruits. For the first time, we define two distinct crumbly phenotypes as part of this work. A consistent crumbly fruit phenotype affecting the majority of fruits every season, which we refer to as crumbly fruit disorder (CFD) and a second phenotype where symptoms vary across seasons as malformed fruit disorder (MFD). Here, segregation of crumbly fruit of the MFD phenotype was examined in a full-sib family and three QTL (Quantitative Trait Loci) were identified on a high density GbS (Genotype by Sequencing) linkage map. This included a new QTL and more accurate location of two previously identified QTLs. A microarray experiment using normal and crumbly fruit at three different developmental stages identified several genes that were differentially expressed between the crumbly and non-crumbly phenotypes within the three QTL. Analysis of gene function highlighted the importance of processes that compromise ovule fertilization as triggers of crumbly fruit. These candidate genes provided insights regarding the molecular mechanisms involved in the genetic control of crumbly fruit in red raspberry. This study will contribute to new breeding strategies and diagnostics through the selection of molecular markers associated with the crumbly trait.

A new species of Glyptapanteles (Hymenoptera: Braconidae: Microgastrinae), a larval parasitoid of Elymnias hypermnestra (Linnaeus) (Lepidoptera: Nymphalidae), along with some new host records of parasitoids from Peninsular India

Zootaxa ◽  
2012 ◽  
Vol 3227 (1) ◽  
pp. 54 ◽  
Author(s):  
ANKITA GUPTA ◽  
BLAISE PEREIRA
Keyword(s):  
New Species ◽  
New Host ◽  
Peninsular India ◽  
Larval Parasitoid ◽  
Hymenopteran Parasitoids ◽  
First Time ◽  
A New Species ◽  
New Host Records

A new species, Glyptapanteles hypermnestrae Gupta and Pereira, is described from Maharashtra, India, and comparedwith closely allied species. This new species was bred from parasitized larvae of Elymnias hypermnestra (Linnaeus) (Lep-idoptera: Nymphalidae). In addition to this, two hymenopteran parasitoids, Apanteles folia Nixon (Braconidae: Microgas-trinae) and Brachymeria indica (Krausse) (Chalcididae), are for first time reported parasitizing larvae of Arhopalaamantes (Hewitson) (Lepidoptera: Lycaenidae) and pupae of Pareronia valeria (Cramer) (Lepidoptera: Pieridae) respectively.

Ascomycete species new to Lithuania

2012 ◽  
Vol 18 (1) ◽  
pp. 35-39 ◽  
Author(s):  
Tatjana Iznova ◽  
Jonė Rukšėnienė
Keyword(s):  
Alnus Glutinosa ◽  
Corylus Avellana ◽  
Rubus Idaeus ◽  
Alluvial Forests ◽  
Dead Branch ◽  
First Time

Abstract Iznova T., Rukšėnienė J., 2012: ascomycete species new to Lithuania [Naujos aukšliagrybių rūšys Lietuvoje]. - Bot. Lith., 18(1): 35-39. In this paper three ascomycete species, Capronia nigerrima, Kalmusia clivensis and Sillia ferruginea, are reported for the first time in Lithuania. The fungi were found on old stromata of pyrenomycetes, bark of dead branch of Corylus avellana and dead stems of Rubus idaeus in the alluvial forests with Alnus glutinosa of the southern, northeastern and eastern Lithuania. Morphological descriptions, comments and illustrations of the species are presented.

scholarly journals High proportion of mixed virus infections in raspberry plantations in Serbia

2020 ◽  
Vol 35 (1) ◽  
pp. 27-37
Author(s):  
Svetlana Paunovic ◽  
Darko Jevremovic
Keyword(s):  
Rubus Idaeus ◽  
Mixed Infections ◽  
Black Raspberry ◽  
Virus Infections ◽  
Red Raspberry ◽  
Rt Pcr ◽  
Necrosis Virus ◽  
Specific Primers ◽  
Leaf Blotch ◽  
First Time

Samples of red raspberry (Rubus idaeus L.) from the most important growing areas in Serbia were analyzed for the presence of four most important raspberry viruses: raspberry leaf blotch virus (RLBV), raspberry leaf mottle virus (RLMV), black raspberry necrosis virus (BRNV) and Rubus yellow net virus (RYNV), and their incidence in individual and mixed infected samples. Seventy-four samples of eight raspberry cultivars, selected from 32 main cultivation sites were tested, of which 62 leaf samples showed symptoms that could be caused by viruses and 12 samples were asymptomatic. The presence of viruses was tested by appropriate RT-PCR and PCR methods, using virus-specific primers. All four viruses were detected with highly significant differences of incidence in a total of 61 infected symptomatic samples. Infection with one of the four tested viruses was detected in 59.0% of all infected samples. The most prevalent was RLBV, which was associated with raspberry leaf blotch disorder in coexistence with its vector raspberry leaf and bud mite P. gracillis, and it was detected in 70.5% of the infected samples. It was followed by RYNV with an incidence of 42.6%, BRNV with 36.1% and RLMV with 9.8% incidence, considering all infected samples. The obtained results for the first time in Serbia showed a high proportion (41.0%) of mixed infections involving between two and four viruses. The most common were infections with two viruses, 68.0% of all mixed-infected samples, and among them coinfections with RLBV and RYNV (44.0%). Viruses of the raspberry mosaic complex, BRNV, RYNV and RLMV, including coinfections of 2-3 listed viruses along with RLBV, were found in a high joined proportion of 14.7% of all infected samples, i.e. 36.0% of mixed infected samples.

scholarly journals Morphometrical and molecular data on plant parasitic nematodes Longidorus attenuatus Hooper, 1961 and L. danuvii Barsi et al., 2007 (Nematoda: Longidoridae) reported from Ukraine for the first time

2016 ◽  
Vol 53 (4) ◽  
pp. 396-400 ◽  
Author(s):  
S. Susulovska ◽  
A. Susulovsky ◽  
F.W. Kornobis
Keyword(s):  
Plant Root ◽  
Nematode Species ◽  
Molecular Data ◽  
Rubus Idaeus ◽  
28S Rdna ◽  
Parasitic Nematodes ◽  
Plant Parasitic Nematodes ◽  
Dry Conditions ◽  
28S Rdna Sequence ◽  
First Time

Summary Genus Longidorus Micoletzky, 1927 consists of obligatory plant root ectoparasites. Some of the genus species are of economic importance as pests of agricultural plants. This paper presents first records of Longidorus attenuatus Hooper, 1961 and L. danuvii Barsi et al., 2007 from Ukraine. Data on morphometrics and D2-D3 28S rDNA region sequence are provided. Additionally, a D2-D3 28S rDNA sequence from a population described previously from Poland was included. For the first time L. danuvii has been found with plants of agricultural importance (Malus domestica Borkh., Pyrus sp. and Rubus idaeus L.). Additionally, this nematode species has been found for the first time in localities with relatively dry conditions (on the tops of the hills), which are distant from banks of rivers – biotopes from which it has been recorded previously.

scholarly journals Clave dicotómica para especies parasitoides e hiperparasitoides (Hymenoptera) de áfidos (Hemiptera: Aphididae) de Costa Rica

2017 ◽  
Vol 28 (3) ◽  
pp. 565 ◽  
Author(s):  
Daniel Zamora Mejías ◽  
Paul E. Hanson
Keyword(s):  
Biological Control ◽  
Costa Rica ◽  
Plant Species ◽  
Biological Control Agents ◽  
Taxonomic Key ◽  
Hymenopteran Parasitoids ◽  
First Time

Aphids represent a major problem for many plant species, due to their ability to serve as pathogen vectors; the use of parasitoids as biological control agents might be a suitable option for long term control of their populations. The objective of this study was to prepare a taxonomic key in order to identify parasitoids and hyperparasitoids of aphids obtained in Costa Rica. In total, during 2008 to 2015, 3009 hymenopteran parasitoids were reared from 25 species of aphids, 2832 (94%) were primary parasitoids and 175 (6%) were hyperparasitoids. A taxonomic key was made for the identification of nine species of primary parasitoids, Braconidae-Aphidiinae, Aphelinidae, Eulophidae, and six species of hyperparasitoids in five families. In addition, the genus Quadrictonus (Aphidiinae), was included in the key and was reported for the first time in this study in Costa Rica.

scholarly journals 180 GENETIC TRANSFORMATION OF RED RASPBERRY (RUBUS IDAEUS L.) WITH A GENE TO CONTROL ETHYLENE BIOSYNTHESIS

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 454f-454 ◽  
Author(s):  
Helena Mathews ◽  
C. Cohen ◽  
W. Wagoner ◽  
J. Kellogg ◽  
V. Dewey ◽  
...  
Keyword(s):  
Marker Gene ◽  
Ethylene Biosynthesis ◽  
Rubus Idaeus ◽  
Selection Marker ◽  
Red Raspberry ◽  
Leaf Petiole ◽  
Selection Medium ◽  
Recycling Pathway ◽  
Transformation Systems ◽  
First Time

We have developed efficient plant rageneration and transformation systems for red raspberry (Rubus idaeus L.). We have successfully introduced a gene for controlling biosynthesis of ethylene into raspberry for the first time. Leaf and petiole segments were co-cultivated with disarmed Agrobacterium strains EHA 101 or 105 containing plasmids pAG5420, pAG 1452 or pAG1552. The plasmids encoded gene sequences for S-adenosylmethionine hydrolase (SAM ase) driven by the fruit specific or wound and fruit specific tomato SE8 or E4 promoters. SAM ase catalyses the conversion of S-adenosylmethionine (SAM) to methylthioadenosine (MTA) and homoserine which can reenter the methionine recycling pathway. SAM is therefore not available for the synthesis of 1-am inocyclopropane carboxylic acid (ACC), the metabolic precursor for ethylene biosynthesis. Initial shoot regenerants were mostly chimeras containing transformed and non-transformed cells. Solid clones of pure transgenics were developed by repeated culture of leaf, petiole and nodal explants of primary regenerants on higher stringency selection medium. Transformants were screened on medium with kanamycin, geneticin or hygromycin depending on the selection marker gene NPTII or hpt. Genomic integration of transgenes were confirmed by Southern hybridization. Transgenic plants of cultivars Canby, Meeker and Chilliwack have been transplanted to the greenhouse for fruit set and further evaluation of transgenic traits.

scholarly journals Effect of Prohexadione-Ca and young canes removal on physiological characteristics of leaf and fruit in raspberry cultivar ‘Willamette’

2013 ◽  
Vol 58 (2) ◽  
pp. 127-138
Author(s):  
Milena Poledica ◽  
Jasminka Milivojevic ◽  
Dragan Radivojevic ◽  
Jelena Dragisic-Maksimovic
Keyword(s):  
Antioxidant Enzyme ◽  
Growth Regulator ◽  
Foliar Application ◽  
Photosynthetic Pigment ◽  
Rubus Idaeus ◽  
Chlorophyll B ◽  
Defense System ◽  
Mean Values ◽  
Photosynthetic Pigment Content ◽  
First Time

The research was designed to evaluate the effect of growth regulator Prohexadione-Ca (ProCa) and young canes removal on the changes of photosynthetic pigment content and modification activities of antioxidant enzyme defense system in leaves and fruits of raspberry cultivar ?Willamette? (Rubus idaeus L.). The experiment was performed in the period of 2011-2012. It consisted of five treatments: control - no treatment; 2ProCa - with 2 ProCa applications; Z - one removal of young canes; Z+2ProCa - young canes removal with 2 ProCa applications; 2Z - two removals of young canes. The first series of young canes were removed for the first time in mid-April and for the second time at the begining of May. In 2ProCa and Z+2ProCa treatments foliar application of ProCa was carried out twice during the period of April-May in interval of three weeks, i.e. when the primocane growth reached 30 cm in height. The following concentrations of ProCa: 125 ppm (first application) and 200 ppm (second application) were applied. Significantly higher mean values of chlorophyll b content were registered in the 2ProCa (42.84 ?g?g-1 FW) and Z+2ProCa (46.12 ?g?g-1 FW) treatment. The highest SOD and POD activities were found in leaves of new young canes which developed after the young canes removal was applied. Z+2ProCa treatment significantly increased CAT activity in raspberry leaves (63.51 U mg-1 prot.). Both of implemented measures, especially applied in combination, resulted in higher activity of CAT, POD and SOD enzymes in the fruits of the examined cultivar.

scholarly journals Effect of Two Biological Formulations Based onBacillus subtilisandPseudomonas fluorescenson Control ofDidymella applanata, the Causal Agent of Red Raspberry Cane Spur Blight

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Margarita Shternshis ◽  
Tatyana Shpatova ◽  
Anatoly Belyaev
Keyword(s):  
Weather Conditions ◽  
Field Trials ◽  
Antagonistic Activity ◽  
Rubus Idaeus ◽  
Causal Agent ◽  
Red Raspberry ◽  
Natural Conditions ◽  
First Time

In vitroandin vivostudies were conducted to estimate the efficacy of the two microbial formulations based onBacillus subtilisCohn. andPseudomonas fluorescensMig. on the fungusDidymella applanata(Niessl.) Sacc., the causal agent of red raspberry (Rubus idaeusL.) spur blight.In vitro, both bacteria reduced the growth ofD. applanata. In inoculation experiments with raspberry canes in two cultivars with different susceptibility toD. applanata, these antagonistic bacteria suppressed fungal development by reducing the lesions area and the number ofD. applanatafruiting bodies. Field trials of two biological formulations under natural conditions showed a significant suppression of the disease.B. subtilisandP. fluorescensincluded in the formulations revealed antagonistic activity towardsD. applanatathat depended on the red raspberry cultivar and weather conditions. In all cases,B. subtilisshowed better results thanP. fluorescensin biocontrol of the raspberry spur blight. This study demonstrated for the first time the ability of the biocontrol agentsB. subtilisandP. fluorescensto suppress red raspberry cane spur blight, a serious worldwide disease.

scholarly journals Mapping of the Waxy Bloom Gene in ‘Black Jewel’ in a Parental Linkage Map of ‘Black Jewel’ × ‘Glen Ample’ (Rubus) Interspecific Population

Agronomy ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1579
Author(s):  
Dora Pinczinger ◽  
Marcel von Reth ◽  
Jens Keilwagen ◽  
Thomas Berner ◽  
Andreas Peil ◽  
...  
Keyword(s):  
High Throughput Sequencing ◽  
De Novo ◽  
Genotyping By Sequencing ◽  
Rubus Idaeus ◽  
Chromosome 2 ◽  
Sequencing Technology ◽  
Breeding Programs ◽  
Rosaceae Family ◽  
Low Costs ◽  
First Time

Black and red raspberries (Rubus occidentalis L. and Rubus idaeus L.) are the prominent members of the genus Rubus (Rosaceae family). Breeding programs coupled with the low costs of high-throughput sequencing have led to a reservoir of data that have improved our understanding of various characteristics of Rubus and facilitated the mapping of different traits. Gene B controls the waxy bloom, a clearly visible epicuticular wax on canes. The potential effects of this trait on resistance/susceptibility to cane diseases in conjunction with other morphological factors are not fully studied. Previous studies suggested that gene H, which controls cane pubescence, is closely associated with gene B. Here, we used tunable genotyping-by-sequencing technology to identify the de novo SNPs of R. occidentalis and R. idaeus using an interspecific population that segregates for the waxy bloom phenotype. We created linkage maps of both species and mapped the identified SNPs to the seven chromosomes (Ro01–Ro07) of Rubus. Importantly, we report, for the first time, the mapping of gene B to chromosome 2 of R. occidentalis using a genetic map consisting of 443 markers spanning 479.76 cM. We observed the poor transferability of R. idaeus SSRs to R. occidentalis and discrepancies in their previously reported chromosome locations.

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