scholarly journals 180 GENETIC TRANSFORMATION OF RED RASPBERRY (RUBUS IDAEUS L.) WITH A GENE TO CONTROL ETHYLENE BIOSYNTHESIS

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 454f-454 ◽  
Author(s):  
Helena Mathews ◽  
C. Cohen ◽  
W. Wagoner ◽  
J. Kellogg ◽  
V. Dewey ◽  
...  
Keyword(s):  
Marker Gene ◽  
Ethylene Biosynthesis ◽  
Rubus Idaeus ◽  
Selection Marker ◽  
Red Raspberry ◽  
Leaf Petiole ◽  
Selection Medium ◽  
Recycling Pathway ◽  
Transformation Systems ◽  
First Time

We have developed efficient plant rageneration and transformation systems for red raspberry (Rubus idaeus L.). We have successfully introduced a gene for controlling biosynthesis of ethylene into raspberry for the first time. Leaf and petiole segments were co-cultivated with disarmed Agrobacterium strains EHA 101 or 105 containing plasmids pAG5420, pAG 1452 or pAG1552. The plasmids encoded gene sequences for S-adenosylmethionine hydrolase (SAM ase) driven by the fruit specific or wound and fruit specific tomato SE8 or E4 promoters. SAM ase catalyses the conversion of S-adenosylmethionine (SAM) to methylthioadenosine (MTA) and homoserine which can reenter the methionine recycling pathway. SAM is therefore not available for the synthesis of 1-am inocyclopropane carboxylic acid (ACC), the metabolic precursor for ethylene biosynthesis. Initial shoot regenerants were mostly chimeras containing transformed and non-transformed cells. Solid clones of pure transgenics were developed by repeated culture of leaf, petiole and nodal explants of primary regenerants on higher stringency selection medium. Transformants were screened on medium with kanamycin, geneticin or hygromycin depending on the selection marker gene NPTII or hpt. Genomic integration of transgenes were confirmed by Southern hybridization. Transgenic plants of cultivars Canby, Meeker and Chilliwack have been transplanted to the greenhouse for fruit set and further evaluation of transgenic traits.

scholarly journals Combining QTL Mapping and Gene Expression Analysis to Elucidate the Genetic Control of ‘Crumbly’ Fruit in Red Raspberry (Rubus idaeus L.)

Agronomy ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 794
Author(s):  
Luca M. Scolari ◽  
Robert D. Hancock ◽  
Pete E. Hedley ◽  
Jenny Morris ◽  
Kay Smith ◽  
...  
Keyword(s):  
Genetic Control ◽  
Developmental Disorder ◽  
Molecular Mechanisms ◽  
Developmental Stages ◽  
Microarray Experiment ◽  
Rubus Idaeus ◽  
Red Raspberry ◽  
Genotype By Sequencing ◽  
First Time ◽  
Selection Of

‘Crumbly’ fruit is a developmental disorder in raspberry that results in malformed and unsaleable fruits. For the first time, we define two distinct crumbly phenotypes as part of this work. A consistent crumbly fruit phenotype affecting the majority of fruits every season, which we refer to as crumbly fruit disorder (CFD) and a second phenotype where symptoms vary across seasons as malformed fruit disorder (MFD). Here, segregation of crumbly fruit of the MFD phenotype was examined in a full-sib family and three QTL (Quantitative Trait Loci) were identified on a high density GbS (Genotype by Sequencing) linkage map. This included a new QTL and more accurate location of two previously identified QTLs. A microarray experiment using normal and crumbly fruit at three different developmental stages identified several genes that were differentially expressed between the crumbly and non-crumbly phenotypes within the three QTL. Analysis of gene function highlighted the importance of processes that compromise ovule fertilization as triggers of crumbly fruit. These candidate genes provided insights regarding the molecular mechanisms involved in the genetic control of crumbly fruit in red raspberry. This study will contribute to new breeding strategies and diagnostics through the selection of molecular markers associated with the crumbly trait.

scholarly journals 575 PB 050 EFFICIENT PLANT REGENERATION FROM LEAF AND PETIOLE EXPLANTS IN RED RASPBERRY

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 514b-514 ◽  
Author(s):  
Cynthia Cohen ◽  
H. Mathews ◽  
V. Dewey ◽  
R. Bestwick
Keyword(s):  
Plant Regeneration ◽  
Average Rate ◽  
Leaf Explants ◽  
Rubus Idaeus ◽  
Regeneration System ◽  
Red Raspberry ◽  
Petiole Explants ◽  
Leaf Petiole ◽  
Rooting Medium ◽  
Modified Ms Medium

Raspberry has very cultivar specific requirements for proliferation. Plant regeneration rates from isolated explants are inconsistent and vary widely among cultivars. As a step towards developing a viable transgenic system in red raspberry (Rubus idaeus L.) we first developed an efficient and consistent protocol for plant regeneration from isolated explants. A modified MS medium with cytokinin BA gave vigorous shoots with an average proliferation rate of 3-5 depending on the cultivar. These vigorous shoot proliferants served as an ideal explant source for plant regeneration experiments. The average rate of shoot regeneration from leaf explants was 72, 32, 68. and 72% for cvs. Canby, Chilliwack, Meeker and Heritage respectively. In addition to leaf, petiole explants were equally good sources for inducing shoot organogenesis. In all the above-mentioned cultivars, 44-57% of the petiole explants gave rise lo healthy and vigorous shoot regenerants in culture. The regenerated shoots were induced lo root on a rooting medium and were successfully transplanted to the greenhouse. This regeneration system was successfully applied in our laboratory for developing gene transfer system in red raspberry (see abstract by Mathews, et al).

scholarly journals High proportion of mixed virus infections in raspberry plantations in Serbia

2020 ◽  
Vol 35 (1) ◽  
pp. 27-37
Author(s):  
Svetlana Paunovic ◽  
Darko Jevremovic
Keyword(s):  
Rubus Idaeus ◽  
Mixed Infections ◽  
Black Raspberry ◽  
Virus Infections ◽  
Red Raspberry ◽  
Rt Pcr ◽  
Necrosis Virus ◽  
Specific Primers ◽  
Leaf Blotch ◽  
First Time

Samples of red raspberry (Rubus idaeus L.) from the most important growing areas in Serbia were analyzed for the presence of four most important raspberry viruses: raspberry leaf blotch virus (RLBV), raspberry leaf mottle virus (RLMV), black raspberry necrosis virus (BRNV) and Rubus yellow net virus (RYNV), and their incidence in individual and mixed infected samples. Seventy-four samples of eight raspberry cultivars, selected from 32 main cultivation sites were tested, of which 62 leaf samples showed symptoms that could be caused by viruses and 12 samples were asymptomatic. The presence of viruses was tested by appropriate RT-PCR and PCR methods, using virus-specific primers. All four viruses were detected with highly significant differences of incidence in a total of 61 infected symptomatic samples. Infection with one of the four tested viruses was detected in 59.0% of all infected samples. The most prevalent was RLBV, which was associated with raspberry leaf blotch disorder in coexistence with its vector raspberry leaf and bud mite P. gracillis, and it was detected in 70.5% of the infected samples. It was followed by RYNV with an incidence of 42.6%, BRNV with 36.1% and RLMV with 9.8% incidence, considering all infected samples. The obtained results for the first time in Serbia showed a high proportion (41.0%) of mixed infections involving between two and four viruses. The most common were infections with two viruses, 68.0% of all mixed-infected samples, and among them coinfections with RLBV and RYNV (44.0%). Viruses of the raspberry mosaic complex, BRNV, RYNV and RLMV, including coinfections of 2-3 listed viruses along with RLBV, were found in a high joined proportion of 14.7% of all infected samples, i.e. 36.0% of mixed infected samples.

scholarly journals Effect of Two Biological Formulations Based onBacillus subtilisandPseudomonas fluorescenson Control ofDidymella applanata, the Causal Agent of Red Raspberry Cane Spur Blight

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Margarita Shternshis ◽  
Tatyana Shpatova ◽  
Anatoly Belyaev
Keyword(s):  
Weather Conditions ◽  
Field Trials ◽  
Antagonistic Activity ◽  
Rubus Idaeus ◽  
Causal Agent ◽  
Red Raspberry ◽  
Natural Conditions ◽  
First Time

In vitroandin vivostudies were conducted to estimate the efficacy of the two microbial formulations based onBacillus subtilisCohn. andPseudomonas fluorescensMig. on the fungusDidymella applanata(Niessl.) Sacc., the causal agent of red raspberry (Rubus idaeusL.) spur blight.In vitro, both bacteria reduced the growth ofD. applanata. In inoculation experiments with raspberry canes in two cultivars with different susceptibility toD. applanata, these antagonistic bacteria suppressed fungal development by reducing the lesions area and the number ofD. applanatafruiting bodies. Field trials of two biological formulations under natural conditions showed a significant suppression of the disease.B. subtilisandP. fluorescensincluded in the formulations revealed antagonistic activity towardsD. applanatathat depended on the red raspberry cultivar and weather conditions. In all cases,B. subtilisshowed better results thanP. fluorescensin biocontrol of the raspberry spur blight. This study demonstrated for the first time the ability of the biocontrol agentsB. subtilisandP. fluorescensto suppress red raspberry cane spur blight, a serious worldwide disease.

Impact of an Open Trellis on Canopy Growth, Light Interception, Yield, and Leaf Physiology of Red Raspberry (Rubus idaeus)

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 468b-468
Author(s):  
Stephen F. Klauer ◽  
J. Scott Cameron ◽  
Chuhe Chen
Keyword(s):  
Leaf Area ◽  
Total Nitrogen ◽  
Dry Weight ◽  
Light Interception ◽  
Rubus Idaeus ◽  
Above Ground Biomass ◽  
Red Raspberry ◽  
Leaf Physiology ◽  
Total Dry Weight ◽  
Upper Third

After promising results were obtained with an open-style split trellis (two top wires) in its initial year, two new trials were established in 1997 in northwest (Lynden) and southwest (Woodland) Washington. For the split trellis, actual yields were 33% (machine-picked 1/2 season) and 17% (hand-picked) greater, respectively, for the two locations compared to the conventional trellis (one top wire). In Woodland, canes from the split trellis had 33% more berries, 55% more laterals, 69% more leaves, and 25% greater leaf area compared with the conventional trellis. Greatest enhancement of these components was in the upper third of the canopy. Laterals were also shorter in this area of the split canopy, but there was no difference in average total length of lateral/cane between trellis types. Total dry weight/cane was 22% greater in the split trellis, but component partitioning/cane was consistent between the two systems with fruit + laterals (43%) having the greatest above-ground biomass, followed by the stem (30% to 33%) and the leaves (21% to 22%). Measurement of canopy width, circumference, and light interception showed that the split-trellis canopy filled in more quickly, and was larger from preanthesis through postharvest. Light interception near the top of the split canopy was 30% greater 1 month before harvest with 98% interception near the top and middle of that canopy. There was no difference between the trellis types in leaf CO2 assimilation, spectra, or fluorescence through the fruiting season, or in total nitrogen of postharvest primocane leaves.

scholarly journals Genome-Wide Association Study (GWAS) for Examining the Genomics Controlling Prickle Production in Red Raspberry (Rubus idaeus L.)

Agronomy ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 27
Author(s):  
Archana Khadgi ◽  
Courtney A. Weber
Keyword(s):  
Association Study ◽  
Genome Wide Association Study ◽  
Genomic Region ◽  
Rubus Idaeus ◽  
Genome Wide Association ◽  
Nucleotide Polymorphisms ◽  
Red Raspberry ◽  
Genome Wide ◽  
A Genome ◽  
Genotype By Sequencing

Red raspberry (Rubus idaeus L.) is an expanding high-value berry crop worldwide. The presence of prickles, outgrowths of epidermal tissues lacking vasculature, on the canes, petioles, and undersides of leaves complicates both field management and harvest. The utilization of cultivars with fewer prickles or prickle-free canes simplifies production. A previously generated population segregating for prickles utilizing the s locus between the prickle-free cultivar Joan J (ss) and the prickled cultivar Caroline (Ss) was analyzed to identify the genomic region associated with prickle development in red raspberry. Genotype by sequencing (GBS) was combined with a genome-wide association study (GWAS) using fixed and random model circulating probability unification (FarmCPU) to analyze 8474 single nucleotide polymorphisms (SNPs) and identify significant markers associated with the prickle-free trait. A total of four SNPs were identified on chromosome 4 that were associated with the phenotype and were located near or in annotated genes. This study demonstrates how association genetics can be used to decipher the genetic control of important horticultural traits in Rubus, and provides valuable information about the genomic region and potential genes underlying the prickle-free trait.

scholarly journals Identification of highly transformable wheat genotypes for mass production of fertile transgenic plants

Genome ◽  
2002 ◽  
Vol 45 (2) ◽  
pp. 421-430 ◽  
Author(s):  
A Pellegrineschi ◽  
L M Noguera ◽  
B Skovmand ◽  
R M Brito ◽  
L Velazquez ◽  
...  
Keyword(s):  
Transformation Efficiency ◽  
Transformation Process ◽  
Plant Genotype ◽  
Donor Plant ◽  
Genotype Effect ◽  
Factors Influencing ◽  
Number Of Factors ◽  
Selection Medium ◽  
Callus Regeneration ◽  
Transformation Systems

The efficiency of wheat biolistic transformation systems strongly depends on the bombardment parameters, the condition of the donor plant, and the plant genotype chosen for the transformation process. This paper analyzes the transformation efficiency of the 129 wheat sister lines generically called 'Bobwhite', originally obtained from the cross 'Aurora'//'Kalyan'/'Bluebird 3'/'Woodpecker'. A number of factors influencing the transformation were examined, such as the ability to produce embryogenic callus, regeneration in selection medium, and overall transformation performance. Of the 129 genotypes evaluated, eight demonstrated transformation efficiencies above 60% (60 independent transgenic events per 100 immature embryos bombarded). Among the eight genotypes identified, we studied agronomic characteristics such as earliness to identify the most adaptable line(s) for different lab conditions. 'Bobwhite' SH 98 26 was identified as a super-transformable wheat line.Key words: wheat transformation, 'Bobwhite', genotype effect.

scholarly journals Performance of Three Primocane-fruiting Red Raspberry Cultivars in Ontario and Québec

1993 ◽  
Vol 118 (3) ◽  
pp. 388-392 ◽  
Author(s):  
Jean-Pierre Privé ◽  
J.A. Sullivan ◽  
J.T.A. Proctor ◽  
O.B. Allen
Keyword(s):  
Maximum Yield ◽  
Rubus Idaeus ◽  
Yield Potential ◽  
Red Raspberry ◽  
Genotype X Environment ◽  
Genotype X Environment Interactions ◽  
Consistent Performance

The influence of genotype x environment interactions on the performance of `Autumn Bliss' `Heritage' and `Redwing' primocane-fruiting (PF) red raspberry (Rubus idaeus L.) cultivars was studied at six sites across Ontario and Quebec during 1989 and 1990. Cultivar × location × year interactions were found for most vegetative and reproductive components analyzed. `Autumn Bliss' had the most consistent performance of the three cultivars in all location/year combinations, while `Redwing' varied greatly between environments. `Heritage' was always the latest-bearing of the three cultivars and failed to achieve its maximum yield potential in many of the northern locations.

scholarly journals Breaking Dormancy in Red Raspberry with Hot Water Treatment and its Effects on Cold Hardiness

2006 ◽  
Vol 131 (2) ◽  
pp. 209-213 ◽  
Author(s):  
Pauliina Palonen ◽  
Leena Lindén
Keyword(s):  
Water Treatment ◽  
Cold Hardiness ◽  
Treatment Temperature ◽  
Rubus Idaeus ◽  
Hot Water ◽  
Bud Dormancy ◽  
Hot Water Treatment ◽  
Red Raspberry ◽  
Breaking Dormancy ◽  
Water Treatments

`Maurin Makea', `Muskoka', ` Ottawa', and `Preussen' red raspberry (Rubus idaeus L.) canes were collected from the field and subjected to different hot water treatments (20, 35, 40, 45, and 50 °C) to determine if endodormancy could be removed by a near lethal stress. Estimation of days for 50% budbreak (DD50) was found useful for describing the state of bud dormancy in the samples. Bud dormancy was broken in `Ottawa' by immersing the canes in 45 °C water for 2 hours, in `Maurin Makea' by treating the canes in 40 °C water, and in `Preussen' by both 40 and 45 °C treatments. The influence of this treatment on dormancy and cold hardiness at different times of the winter was further examined using `Ottawa' raspberry. The treatment removed bud dormancy most effectively in October, when the samples were in deepest dormancy. A slight effect was observed in November, but no effect in January. During ecodormancy in February the treatment delayed budbreak. Hot water treatment reduced cold hardiness of `Ottawa' canes by 8 to 15 °C, and that of buds by 9 to 13 °C during both endo- and ecodormancy. Based on the capacity of buds and canes to reacclimate, recovery from the stress treatment was possible at temperatures ≥4 °C. Loss of cold hardiness was caused by high treatment temperature itself and was not related to breaking of dormancy in samples. This finding suggests that dormancy and cold hardiness are physiologically unconnected in raspberry.

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